pgap1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pgap1
Entrez Gene ID	111580614
Full Name	post-GPI attachment to proteins 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023288436.1	XP_023144204.1	GPI inositol-deacylase

Pathways from BioSystems
Gene Ontology

The following pgap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pgap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm92194	XM_023288436.1 Latest version!	Amphiprion ocellaris post-GPI attachment to proteins 1 (pgap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pgap1 ( XM_023288436.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023144204.1 Amphiprion ocellaris post-GPI attachment to proteins 1 (pgap1), mRNA.

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CloneID	OAm92194
Clone ID Related Accession (Same CDS sequence)	XM_023288436.1
Accession Version	XM_023288436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	GPI inositol-deacylase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373433.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTCG CCACTGTGGC CTTTTACGGT GTCGCTCTGG GACTGCTGGC GGTTGGCCTG 
CGAGAGTTGC TAACTGGCTT CGAGGAGAAC AGATGCAGTA TGACCTATAT GTTTGAATAC 
CCGGAGTATC GGCGTATTGC TCTGCCTCGT CGTGTGGCCA GACTGTACCC AGCATACGGC 
CTCTACCTGT ACGGAGAGGG TCTGTACGCC CAGGAGACCC GGACTCTTAA ACTCACCGGT 
GCCCCTGTGC TTTTCCTGCC TGGAAATGCA GGAAGTTATA AACAAGCTCG CTCTCTGGGC 
TCGGTCGCCT TGAGGAAGGC TGAAAACATG GAAGGAGGTC TCCACTTCAA CGTGTTCACC 
GTGGACTTCA ACGAGGAGCT GGTGGCCCTT TACGGAGGCA GTTTGCTCAG GCAGACACAC 
TTCCTGCATG AGAGCATTAA AGCCATCCTG AGGCTCTACA AGCACCTGAA GAGCCCTCCT 
CAGAGCGTAG TGCTCGTTGG TCACTCCATG GGAGGAGTGG TGGCCCGAGC GCTGTTCACT 
TTGCCTCGCT TCAACACTAA TCTGGTCAGC CTCATCATCA CCCAGGCCTC CCCTCACCAG 
GCTCCAGTGC TGGCCCTCGA CCCATACCTG CTGGATTTCT ACTCTGCTGT TAGACAGAAG 
TGGGTGAACC AGGCGAACAA GCTCAGGAAT GTCACGGTTT TATCAGTCGG AGGTGGTTAC 
CGTGACTACC AGGTCCGTTC TGGCCTGACT TCCCTTCCTT GTCCCCGAGG AGACATTAAT 
AAGCTGTCAC TGGTGGTAAC CACAGTTCCC AGGACATGGG TGTCCACCGA TCATCTATCC 
ATTGTTTGGT GCAAAGAGCT GGTTCTTGCT ACTGTCAGGG CGTTTTTTGA CCTCATCGAT 
CCCGAAACCA GACAGTTCAC AGAAAACCTC GAGAAGAAAA TGACTGTGCT CAACCATCAT 
TTCATCAGAC ACCCTGTCAG GATGCTGGGA GAAACCCAAG AAACCTCCAT CTCAATCCTA 
GATTTTCCTG AGGCATGGAG TGAAGTTAAC ACACTACGTC TGGCGTACAG CACTCCAAAG 
GAAGGACAAG TCAAATACTT TATGTTTGCT CTGTCGAGTC GCAGGAAAGC TTACAGTCAT 
TTCTACTGCC GGAGCAACAA CCTGGAGATG ACTAGCTGGG TGTATGGCTG TGTGCACAAG 
AACGGTTCTT CATGTGTGCA TGCAGTGGAT CTGTCAATGG GAACTGAGCT TCTGCCTCCA 
TACAAGGTCC TGATTCTGAG TCTCAGTGAC TTGTCTTCAG TTACTCATCT GGTTGTTTCG 
GCCTCCAACC TCAATGGCAG ACAGTTCACA GTGGAGTGTG AGTGGCAGAG ACAAGAATCT 
CAGACTTTAT CTGTCCCAGT ACCACATGTC CTTTCCTTAG GTGTGACTGC CAGCGAGATT 
ACAGTCAACT CCTCTGGCCT TCTTCACTCT ATTGAGCTGC AACACTTTCA CCAAGTCTAC 
CAGGCTTTCA GAATTAATGT TGCAAGCCAG TGCAAAGTGA ATAAAGATAG ATTGCCAAAT 
GTGTACAGGA TAAAAGTAGC GTGGTTTCGA GAGGACTCTT TAACTACAGT TAGTGTACCA 
TCAGTGACAG AAATCTCAGG GATGCTACAT ACAAGTCGTC CAGACAATAC CTCAGGTGTC 
CTCCTGCAGC TCCACACTGC TCCCAACTGC CAGTATAAGG TGTCAATAAG AACTTCATTA 
CCGAAGGTTC TGGGACAGGT ACTGAGGTTC TGTGGTCCCA TGGTGCCGGT CTACACCGCT 
GTAACCCTCC TCCTGGCCTG TGTGGGGCAG CTGTCCTCAA TCAGGAAATC GAAGAGAGCT 
GCAGCTATGA GCCTGGTCGT GGCCAAAGGC TTGCAGCCTC ACAAAGTCAA CGTATCTGTT 
TACATTCTGC ACATCTTACT GAGCTGTAGC TGTTTTCTTG AGGTACGGTC CTTTCTTTTC 
CTCCCACCTA TGGATACACT CCCCCCAACA TTCCCTGACG GGACATTTCA TGAAGGAGTC 
CCACCGGTTG AAGAATGGCC CCACCTTCTT TCCCCTCTGC TCTACATCTT GGGGGCAGCT 
GTGGCATACT GGGGCAGCAC ACTACTCCGT CTCATTATAC GACTGATCTC ATTAATATTA 
GCTCCACTGC ACAGACCGTC TGTGTCTAGA GACTGTGGCA CCTTGCGACT GCGAACCCAG 
ATCCTCATCA CTGTGGGTCT GGCGGTTACG GGTGGGACGT TCTGTGGGGC GCTCTCAATC 
TTTGTCTCCT TTCTGTTCCA CCTTTATAGG GTACTAAGGC TGCAGATGAC AGAAAGGTCT 
TTGAGTCACA TGCTGAATCT GGCACCCCGG AAGCACAAAG AAGCTGAAAA CGGCACACTT 
GATGCTGAAC CCTTGAGAAA GACTAAAGAA TGTAGCAGCG CCCCCCTGCT GTCTGAGTGC 
GCTCTGCAGG AGGTGAGGGA TGACCTGCAG ATCCACCTCT GCCTGTCAGC GCTCTTCACG 
CTACCTGTCA TGCTCAGCGC ACCCTCCTTT ATCCACTGGA TCCGCAATCT GAGGTACTCC 
ACCCAGTTGG ATCCTGATCC TTGTTGGCCA CACATTGTGC CTCTAATTAT TGTCTACATG 
TTGCTCATTA ACTGCAACAC TTTAAAACTC AGCAACAGTA AACTCCTGCC TCTGGCCTCC 
AGCCTCCCTC TCCCTTTGGC CATCACCATG GTGACCTTCT CTCCACTTCA CCTCTACAGA 
GTCGTCTACT TCTTGTTGGC AGCACTCGTC CCTATGGCCT TGTGTTGTCT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023144204.1
CDS	95..2911
Translation	MKLATVAFYGVALGLLAVGLRELLTGFEENRCSMTYMFEYPEYRRIALPRRVARLYPAYGLYLYGEGLYAQETRTLKLTGAPVLFLPGNAGSYKQARSLGSVALRKAENMEGGLHFNVFTVDFNEELVALYGGSLLRQTHFLHESIKAILRLYKHLKSPPQSVVLVGHSMGGVVARALFTLPRFNTNLVSLIITQASPHQAPVLALDPYLLDFYSAVRQKWVNQANKLRNVTVLSVGGGYRDYQVRSGLTSLPCPRGDINKLSLVVTTVPRTWVSTDHLSIVWCKELVLATVRAFFDLIDPETRQFTENLEKKMTVLNHHFIRHPVRMLGETQETSISILDFPEAWSEVNTLRLAYSTPKEGQVKYFMFALSSRRKAYSHFYCRSNNLEMTSWVYGCVHKNGSSCVHAVDLSMGTELLPPYKVLILSLSDLSSVTHLVVSASNLNGRQFTVECEWQRQESQTLSVPVPHVLSLGVTASEITVNSSGLLHSIELQHFHQVYQAFRINVASQCKVNKDRLPNVYRIKVAWFREDSLTTVSVPSVTEISGMLHTSRPDNTSGVLLQLHTAPNCQYKVSIRTSLPKVLGQVLRFCGPMVPVYTAVTLLLACVGQLSSIRKSKRAAAMSLVVAKGLQPHKVNVSVYILHILLSCSCFLEVRSFLFLPPMDTLPPTFPDGTFHEGVPPVEEWPHLLSPLLYILGAAVAYWGSTLLRLIIRLISLILAPLHRPSVSRDCGTLRLRTQILITVGLAVTGGTFCGALSIFVSFLFHLYRVLRLQMTERSLSHMLNLAPRKHKEAENGTLDAEPLRKTKECSSAPLLSECALQEVRDDLQIHLCLSALFTLPVMLSAPSFIHWIRNLRYSTQLDPDPCWPHIVPLIIVYMLLINCNTLKLSNSKLLPLASSLPLPLAITMVTFSPLHLYRVVYFLLAALVPMALCCLL

Target ORF information:

RefSeq Version	XM_023288436.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris post-GPI attachment to proteins 1 (pgap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023288436.1

ORF Insert Sequence:

ATGAAACTCG CCACTGTGGC CTTTTACGGT GTCGCTCTGG GACTGCTGGC GGTTGGCCTG 
CGAGAGTTGC TAACTGGCTT CGAGGAGAAC AGATGCAGTA TGACCTATAT GTTTGAATAC 
CCGGAGTATC GGCGTATTGC TCTGCCTCGT CGTGTGGCCA GACTGTACCC AGCATACGGC 
CTCTACCTGT ACGGAGAGGG TCTGTACGCC CAGGAGACCC GGACTCTTAA ACTCACCGGT 
GCCCCTGTGC TTTTCCTGCC TGGAAATGCA GGAAGTTATA AACAAGCTCG CTCTCTGGGC 
TCGGTCGCCT TGAGGAAGGC TGAAAACATG GAAGGAGGTC TCCACTTCAA CGTGTTCACC 
GTGGACTTCA ACGAGGAGCT GGTGGCCCTT TACGGAGGCA GTTTGCTCAG GCAGACACAC 
TTCCTGCATG AGAGCATTAA AGCCATCCTG AGGCTCTACA AGCACCTGAA GAGCCCTCCT 
CAGAGCGTAG TGCTCGTTGG TCACTCCATG GGAGGAGTGG TGGCCCGAGC GCTGTTCACT 
TTGCCTCGCT TCAACACTAA TCTGGTCAGC CTCATCATCA CCCAGGCCTC CCCTCACCAG 
GCTCCAGTGC TGGCCCTCGA CCCATACCTG CTGGATTTCT ACTCTGCTGT TAGACAGAAG 
TGGGTGAACC AGGCGAACAA GCTCAGGAAT GTCACGGTTT TATCAGTCGG AGGTGGTTAC 
CGTGACTACC AGGTCCGTTC TGGCCTGACT TCCCTTCCTT GTCCCCGAGG AGACATTAAT 
AAGCTGTCAC TGGTGGTAAC CACAGTTCCC AGGACATGGG TGTCCACCGA TCATCTATCC 
ATTGTTTGGT GCAAAGAGCT GGTTCTTGCT ACTGTCAGGG CGTTTTTTGA CCTCATCGAT 
CCCGAAACCA GACAGTTCAC AGAAAACCTC GAGAAGAAAA TGACTGTGCT CAACCATCAT 
TTCATCAGAC ACCCTGTCAG GATGCTGGGA GAAACCCAAG AAACCTCCAT CTCAATCCTA 
GATTTTCCTG AGGCATGGAG TGAAGTTAAC ACACTACGTC TGGCGTACAG CACTCCAAAG 
GAAGGACAAG TCAAATACTT TATGTTTGCT CTGTCGAGTC GCAGGAAAGC TTACAGTCAT 
TTCTACTGCC GGAGCAACAA CCTGGAGATG ACTAGCTGGG TGTATGGCTG TGTGCACAAG 
AACGGTTCTT CATGTGTGCA TGCAGTGGAT CTGTCAATGG GAACTGAGCT TCTGCCTCCA 
TACAAGGTCC TGATTCTGAG TCTCAGTGAC TTGTCTTCAG TTACTCATCT GGTTGTTTCG 
GCCTCCAACC TCAATGGCAG ACAGTTCACA GTGGAGTGTG AGTGGCAGAG ACAAGAATCT 
CAGACTTTAT CTGTCCCAGT ACCACATGTC CTTTCCTTAG GTGTGACTGC CAGCGAGATT 
ACAGTCAACT CCTCTGGCCT TCTTCACTCT ATTGAGCTGC AACACTTTCA CCAAGTCTAC 
CAGGCTTTCA GAATTAATGT TGCAAGCCAG TGCAAAGTGA ATAAAGATAG ATTGCCAAAT 
GTGTACAGGA TAAAAGTAGC GTGGTTTCGA GAGGACTCTT TAACTACAGT TAGTGTACCA 
TCAGTGACAG AAATCTCAGG GATGCTACAT ACAAGTCGTC CAGACAATAC CTCAGGTGTC 
CTCCTGCAGC TCCACACTGC TCCCAACTGC CAGTATAAGG TGTCAATAAG AACTTCATTA 
CCGAAGGTTC TGGGACAGGT ACTGAGGTTC TGTGGTCCCA TGGTGCCGGT CTACACCGCT 
GTAACCCTCC TCCTGGCCTG TGTGGGGCAG CTGTCCTCAA TCAGGAAATC GAAGAGAGCT 
GCAGCTATGA GCCTGGTCGT GGCCAAAGGC TTGCAGCCTC ACAAAGTCAA CGTATCTGTT 
TACATTCTGC ACATCTTACT GAGCTGTAGC TGTTTTCTTG AGGTACGGTC CTTTCTTTTC 
CTCCCACCTA TGGATACACT CCCCCCAACA TTCCCTGACG GGACATTTCA TGAAGGAGTC 
CCACCGGTTG AAGAATGGCC CCACCTTCTT TCCCCTCTGC TCTACATCTT GGGGGCAGCT 
GTGGCATACT GGGGCAGCAC ACTACTCCGT CTCATTATAC GACTGATCTC ATTAATATTA 
GCTCCACTGC ACAGACCGTC TGTGTCTAGA GACTGTGGCA CCTTGCGACT GCGAACCCAG 
ATCCTCATCA CTGTGGGTCT GGCGGTTACG GGTGGGACGT TCTGTGGGGC GCTCTCAATC 
TTTGTCTCCT TTCTGTTCCA CCTTTATAGG GTACTAAGGC TGCAGATGAC AGAAAGGTCT 
TTGAGTCACA TGCTGAATCT GGCACCCCGG AAGCACAAAG AAGCTGAAAA CGGCACACTT 
GATGCTGAAC CCTTGAGAAA GACTAAAGAA TGTAGCAGCG CCCCCCTGCT GTCTGAGTGC 
GCTCTGCAGG AGGTGAGGGA TGACCTGCAG ATCCACCTCT GCCTGTCAGC GCTCTTCACG 
CTACCTGTCA TGCTCAGCGC ACCCTCCTTT ATCCACTGGA TCCGCAATCT GAGGTACTCC 
ACCCAGTTGG ATCCTGATCC TTGTTGGCCA CACATTGTGC CTCTAATTAT TGTCTACATG 
TTGCTCATTA ACTGCAACAC TTTAAAACTC AGCAACAGTA AACTCCTGCC TCTGGCCTCC 
AGCCTCCCTC TCCCTTTGGC CATCACCATG GTGACCTTCT CTCCACTTCA CCTCTACAGA 
GTCGTCTACT TCTTGTTGGC AGCACTCGTC CCTATGGCCT TGTGTTGTCT CCTCTGA