eif2d cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2d
Entrez Gene ID	111576839
Full Name	eukaryotic translation initiation factor 2D
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023282777.1	XP_023138545.1	eukaryotic translation initiation factor 2D

Pathways from BioSystems
Gene Ontology

The following eif2d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm87736	XM_023282777.1 Latest version!	Amphiprion ocellaris eukaryotic translation initiation factor 2D (eif2d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2d ( XM_023282777.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023138545.1 Amphiprion ocellaris eukaryotic translation initiation factor 2D (eif2d), mRNA.

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CloneID	OAm87736
Clone ID Related Accession (Same CDS sequence)	XM_023282777.1
Accession Version	XM_023282777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	eukaryotic translation initiation factor 2D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373010.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCA AAGCGTTTCG TGTCAAATCA AACACTGTAA TCAAAGGATC AGACAGGAGA 
AAGCTCAAAG CGGACGTATC TGCAGCCTTT CCTTCACTGT CTGCTGATGA ACTGTCTGAG 
CTGATCCCCA ACAAAGAGGA ATTAAATGTT GTGAAGATTT ATGCACACAA GGGAGAAGCT 
GTGACAGTTT ATGTTCTTCA CAAAAACCCA CTGTTCTTTG AACTGGAGAA ACGACTTTAT 
CCCACAGTGT ATGTGCTTTG GCGTTATCCT AATCTCCTAC CAACATTCAG GACATGGCCA 
CCTGTGCTTG AAAAGTTGAT TGGAGGGGCA GATCTCATGC TGCCAGGTGT GGTGGTGCCG 
TCATCTGGTC TCCCAGATGT GAAACAGGGA GACTGCTGTG CCGTTACAGT TGCAAGCAAT 
GGAGCTCCTG TTGCACTTGG CACAGCTGCA GTTTCCAGTG CAGAGATGTA CAGTTTGGGT 
ATGAAAGGCA GAGGATTGTG TGTTCTCCAC ACATACATGG ACTATCTTTG GGCATTTGGA 
GATAAGTCGG GTCCTCCTTC TTTACCGGAT GCCGAAAGTG AAGGACAAGA AGTAGATGAA 
GTGGAAGATG ACATCAATGA GAAAGAGGAG GACGATGTGG TTGAAAAGTG TGTAGAGGAG 
CAAGAGAGCT CTGGTGAATC TGCCATGAAT CGAACCTGTT CTGGTGTTGA GGAGCTGAGA 
TTAGCTGAGA AAGAGGAAGA GAAGGATGAA AAGGGCAGTG AGAAGGAGGA GGAGAACCAG 
GATGACCAGA AAACCCCACA AGAGATAATG GACGCCCTGT TGTTGCGGTG CTTTCTGCAT 
GCTCTCAAGA GCAAGGTGAA GAAGTCAGAG CTCCCTCTGC TGACCAGTAC ATTTCTCCGC 
ATCCACATGT TCTCCTGCTG CCCAAGTGGA AAACAGCTTG ATATTAAGAA ATCCAGCTAT 
AAAAAGCTTT CCAAGTTTCT CCAGGTTATG CAGCAGCAGC ATCATCTTGT GCGAGTTAAA 
GAACTCACCA AGGGTGTGGA GAGCATTGTG GAGGTAGACT GGAAAAATCC AGAATTGCGC 
TCCTTTAGCA TTCCAGAAGA GACAGATGTT GAAGCAGCTG TGGTGCAGGA TGGAGGGGAA 
GGAGAAATAA CTTACCGTCC TCCAGAGATC ACAACTCTTT ACTCTGTGCC TGCACGGCTG 
GAGCCTCTCT TTTTGGATGC GAACAAGAGG AAAGGAACAA CACTGCATGC TGCTGAAGTG 
AGAAGCATCA TCACAGAATA TGTGAAGAAA AATGAACTGG TGGATGAAAA TAATAAGAAT 
TATGTGACCA TCAATCCTAC TCTATGTGAT TGCTTGCTGG AAAAATCTGA ATACCAGGAG 
GTAGAGTCTC TCAAGTGGGA TGACCTGTTC AGCAGGACAA TGGGGCGGAT GCAGGAGTGT 
TATCAAGTTG TATTCCCTGG ACAAGCATCT ATAATAAAGA AGGGCCATAT TGAGCCTATA 
GACATCTCTG TGGCTTCTCG AGGCTCCAAT AAGAAGGTTA CTCTGATAAA GAATCTGGAA 
GTGTATGGTT TGGATCCTGC AGTTGTGGCC ACTGCTTTGC AGCACAGAGT TCAGGCCAGC 
TCTGTCTTAC ATCAAATCCC GGGAGCCAAG GACAAAGTCC TGGTCCAGAT CCAAGGCAAC 
CAAATTCATC AAGTCGGCAA TCTGCTTCTT GATCATTATC AAATCCCACG CAAGTACATC 
CAGGGACTAG ACAAAGCTCC AAAAAGCGGC AAGAAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023138545.1
CDS	185..1966
Translation	MFAKAFRVKSNTVIKGSDRRKLKADVSAAFPSLSADELSELIPNKEELNVVKIYAHKGEAVTVYVLHKNPLFFELEKRLYPTVYVLWRYPNLLPTFRTWPPVLEKLIGGADLMLPGVVVPSSGLPDVKQGDCCAVTVASNGAPVALGTAAVSSAEMYSLGMKGRGLCVLHTYMDYLWAFGDKSGPPSLPDAESEGQEVDEVEDDINEKEEDDVVEKCVEEQESSGESAMNRTCSGVEELRLAEKEEEKDEKGSEKEEENQDDQKTPQEIMDALLLRCFLHALKSKVKKSELPLLTSTFLRIHMFSCCPSGKQLDIKKSSYKKLSKFLQVMQQQHHLVRVKELTKGVESIVEVDWKNPELRSFSIPEETDVEAAVVQDGGEGEITYRPPEITTLYSVPARLEPLFLDANKRKGTTLHAAEVRSIITEYVKKNELVDENNKNYVTINPTLCDCLLEKSEYQEVESLKWDDLFSRTMGRMQECYQVVFPGQASIIKKGHIEPIDISVASRGSNKKVTLIKNLEVYGLDPAVVATALQHRVQASSVLHQIPGAKDKVLVQIQGNQIHQVGNLLLDHYQIPRKYIQGLDKAPKSGKKK

Target ORF information:

RefSeq Version	XM_023282777.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris eukaryotic translation initiation factor 2D (eif2d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023282777.1

ORF Insert Sequence:

ATGTTTGCCA AAGCGTTTCG TGTCAAATCA AACACTGTAA TCAAAGGATC AGACAGGAGA 
AAGCTCAAAG CGGACGTATC TGCAGCCTTT CCTTCACTGT CTGCTGATGA ACTGTCTGAG 
CTGATCCCCA ACAAAGAGGA ATTAAATGTT GTGAAGATTT ATGCACACAA GGGAGAAGCT 
GTGACAGTTT ATGTTCTTCA CAAAAACCCA CTGTTCTTTG AACTGGAGAA ACGACTTTAT 
CCCACAGTGT ATGTGCTTTG GCGTTATCCT AATCTCCTAC CAACATTCAG GACATGGCCA 
CCTGTGCTTG AAAAGTTGAT TGGAGGGGCA GATCTCATGC TGCCAGGTGT GGTGGTGCCG 
TCATCTGGTC TCCCAGATGT GAAACAGGGA GACTGCTGTG CCGTTACAGT TGCAAGCAAT 
GGAGCTCCTG TTGCACTTGG CACAGCTGCA GTTTCCAGTG CAGAGATGTA CAGTTTGGGT 
ATGAAAGGCA GAGGATTGTG TGTTCTCCAC ACATACATGG ACTATCTTTG GGCATTTGGA 
GATAAGTCGG GTCCTCCTTC TTTACCGGAT GCCGAAAGTG AAGGACAAGA AGTAGATGAA 
GTGGAAGATG ACATCAATGA GAAAGAGGAG GACGATGTGG TTGAAAAGTG TGTAGAGGAG 
CAAGAGAGCT CTGGTGAATC TGCCATGAAT CGAACCTGTT CTGGTGTTGA GGAGCTGAGA 
TTAGCTGAGA AAGAGGAAGA GAAGGATGAA AAGGGCAGTG AGAAGGAGGA GGAGAACCAG 
GATGACCAGA AAACCCCACA AGAGATAATG GACGCCCTGT TGTTGCGGTG CTTTCTGCAT 
GCTCTCAAGA GCAAGGTGAA GAAGTCAGAG CTCCCTCTGC TGACCAGTAC ATTTCTCCGC 
ATCCACATGT TCTCCTGCTG CCCAAGTGGA AAACAGCTTG ATATTAAGAA ATCCAGCTAT 
AAAAAGCTTT CCAAGTTTCT CCAGGTTATG CAGCAGCAGC ATCATCTTGT GCGAGTTAAA 
GAACTCACCA AGGGTGTGGA GAGCATTGTG GAGGTAGACT GGAAAAATCC AGAATTGCGC 
TCCTTTAGCA TTCCAGAAGA GACAGATGTT GAAGCAGCTG TGGTGCAGGA TGGAGGGGAA 
GGAGAAATAA CTTACCGTCC TCCAGAGATC ACAACTCTTT ACTCTGTGCC TGCACGGCTG 
GAGCCTCTCT TTTTGGATGC GAACAAGAGG AAAGGAACAA CACTGCATGC TGCTGAAGTG 
AGAAGCATCA TCACAGAATA TGTGAAGAAA AATGAACTGG TGGATGAAAA TAATAAGAAT 
TATGTGACCA TCAATCCTAC TCTATGTGAT TGCTTGCTGG AAAAATCTGA ATACCAGGAG 
GTAGAGTCTC TCAAGTGGGA TGACCTGTTC AGCAGGACAA TGGGGCGGAT GCAGGAGTGT 
TATCAAGTTG TATTCCCTGG ACAAGCATCT ATAATAAAGA AGGGCCATAT TGAGCCTATA 
GACATCTCTG TGGCTTCTCG AGGCTCCAAT AAGAAGGTTA CTCTGATAAA GAATCTGGAA 
GTGTATGGTT TGGATCCTGC AGTTGTGGCC ACTGCTTTGC AGCACAGAGT TCAGGCCAGC 
TCTGTCTTAC ATCAAATCCC GGGAGCCAAG GACAAAGTCC TGGTCCAGAT CCAAGGCAAC 
CAAATTCATC AAGTCGGCAA TCTGCTTCTT GATCATTATC AAATCCCACG CAAGTACATC 
CAGGGACTAG ACAAAGCTCC AAAAAGCGGC AAGAAGAAGT AA