sema6a cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema6a
Entrez Gene ID	111564684
Full Name	semaphorin 6A
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023264406.1	XP_023120174.1	semaphorin-6A isoform X1
XM_023264407.1	XP_023120175.1	semaphorin-6A isoform X2

Pathways from BioSystems
Gene Ontology

The following sema6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm103658	XM_023264407.1 Latest version!	Amphiprion ocellaris semaphorin 6A (sema6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm103657	XM_023264406.1 Latest version!	Amphiprion ocellaris semaphorin 6A (sema6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema6a ( XM_023264407.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023120175.1 Amphiprion ocellaris semaphorin 6A (sema6a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm103658
Clone ID Related Accession (Same CDS sequence)	XM_023264407.1
Accession Version	XM_023264407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	semaphorin-6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCAG AGGCCTTGTT ACTCTACTTC ACGCTACTGC AAATAGCCGG GGCGGGCTTC 
CCCGAAGACA GCGAACCTAT TAGCATCTCA CACGGCAACT ACACAAAGCA GTATCCAGCA 
TTTGTAGGCC ACAAACCTGG AAGGAACAAC ACGCAGCGGC ACAAACTGGA CATCCAGCTC 
ATCATGATCA TGAATAGGAC ATTATACATC GCTGCCAGGG ATCACATATA CACGGTGGAC 
ACGGACACTG CCAACAGTGA CGAGATCTTT TTCAGTAAGA AAATGACATG GAAGTCCAGA 
CAGGCAGATG TGGACACCTG CAGAATGAAA GGGAAGCATA AGGATGAGTG TCACAATTTC 
ATCAAAGTCC TCCTGCAGCA AAATGACGAC ACCTTGTTTG TTTGCGGAAC GAATGCTTTC 
AACCCTTCCT GTCGAACCTA CAAGATGGAC ACCCTGGAGG CTCTGGGAGA GGAGATCAGT 
GGGATGGCAC GCTGTCCGTA TGATGCCAAG CACGCCAACG TCGCCCTCTT TGCTGATGGC 
AAGCTGTACT CGGCCACCGT AACAGACTTT CTCGCGATCG ATGCGGTCAT CTATCGTAGC 
CTTGGAGACA GCCCGACTCT GCGCACTGTC AAACACGACT CCAAATGGCT GAAAGAGCCC 
TATTTCGTCC AGGCTGTCGA TTATGGGGAC TTCATCTACT TCTTCTTCCG CGAAATTGCT 
ATGGAGTACA ACACGATGGG AAAGGTGGTG TTTCCTCGGG TGGCAAGGGT TTGTAAGAAC 
GACCGAGGAG GCTCGCCTCG TGTCCTGGAG AAGCAGTGGA CCTCGTTTCT CAAGTCTCGG 
ATGAACTGCT CCATCCCCGG CGACTCCCAT TTCTACTTCA ACATCCTGCA GGCAGTAACG 
GACGTCATTC ACATCAATGG GAGAGATGTC GTCATGGCAA CTTTCTCCAC TCCTTACAAC 
AGTATTCCAG GTTCGGCTGT GTGTGCCTAT GACATGGCGG AGGTGGCCAA CACGTTCACG 
GGGCGTTTCA AAGAGCAGAA ATCCCCAGAC TCCACCTGGA CGCCTTTTCC CGAAGAGAAG 
GTTCCAAAGC CGAGGCCTGG GAACTGTGCC GGCACTCCAT CCATGGAGAG GTACAAGGTA 
TCCAATGAGT TCCCCGACGA CACACTCAAC TTCATCAAGA TGCATCCTCT GATGGATGAG 
GCCGTGCCCT CCATCGCTAA CAGGCCCTGG TTTCTCAAGA CCATGGTTCG GTATCGGCTG 
ACGCGCATCG TGGTGGACAA CGAGGCCGGT CCCAACAAGA ACCACACAGT GGTGTTCCTG 
GGATCTGAGA AAGGCATCAT CCTCAAGTTC TTGGCCAAAA TGAATAATGG TTTCCTCAAC 
GACAGCCTGT TTTTAGAGGA GCTCAACGTC TACAACCCTG ACAAGTGCAG TATAGATGGT 
GTGGAAGACA AGCGTATCGT CGGTATGCAG ATAGACAGCA AGAGCCATGC TCTGTGGGTC 
GCCTTCACTT CCTGTGTGGT CAAAGTGCCA CTGTCACGCT GTGAAAGGCA CGGACGATGC 
AAGAAGTCCT GTATAGCCTC CAGAGACCCG TACTGTGGCT GGGTATCAGA AGGGGCCTGC 
AGGCAGGTGG TTGGCAACAC CAAATCAGCC TTTGAGCAGG ACGTGGAGCA TGGTAACACA 
GATGGCCTGG GAGACTGCCA AAATACCTTT GTGGCTCTGA ACGGTGTGAT CCGTGAGAGC 
TACCAGAAGG GTCGAGACCA GCTCGTTCCT GTGACCCTGT TGGCCATCGC CGTTATCCTA 
GCGTTTGCGA TGGGTGCCAT CTTCTCTGGC ATCATCGTCT ACTGTGTCTG TGACCACCGG 
CGGCGAGACA TCGACCTGAC AGGCCGCAAG GAAAAGGACA GCCACCACCT ACACTCCCGC 
CGGGGATCCA TGAACAGTGT GACCAAGCTC ACCGGCCTGT TTGAGACCCA GGCCAAAGAC 
GGTCGCCCCG AGGCCATCCT CACCCCTTTG ATGCACAACG GGAGACTGAC ACCAAATGGG 
AAGATGCTGA TAAAAGCAGA CCAGCATCAC CTGGACCTTG CTGCTCTGCC AACGCCTGAA 
TCCACTCCCA TGCAGCCTCG TCGTAAGCCC AGCCGGGGCA GCCGTGAGTG GGAGAGAAAC 
CAGAACCTCA TCAACGCTTG CACCAAGGAC ATGCCACCTA TGGGCTCCCC TGTCATCTCT 
ACAGACCTGC CCCTGAGGGC CTCACCAGGA CACATCCCAA GCGTGGTAGT CCTACCTCTG 
CCGCAGCATC AGCACCAGCT CCAGCCTCAT CTGCAGCAGC ACCAGCAGTC CTACCAGCAT 
GAGTACGTGG AGCAGCCACA TCACAGCGAC CTGAGCATGG GCCACGGTGT CATGGATGAC 
CAGGGAGCCA CCCTGGAGTA CAAGACAGTG AAGAGCCCAT GTCACAACCT CAACATGGTG 
GATCCAGATG GGGTGCTGCC ACCTCGCGTG CCCCAACGAG AGGCCTCCCT TACAATCCCT 
CCTGCTGTCC CACAGATGGG CAAACGCCTG GAGGTCCACT CGTCGGTCTA CGGGCTCCGG 
AAAGGATCAG CGTCTGCTGC TTCTGGATCA TCCGCCCTAA AGAAGCACAA CACCAACTCA 
TCTAACTCCT CCCATTTGGC GAGACATCAC AGCTTCAACC GTGTGGAGAC TCCACCGCCT 
GCACCTCAGC GGGTGGACTC CATACAAATG ACAGCACAGG GCATATCTCT GTCCCGGCAG 
CCTGGAATGA GTTCCTACGG TTCACTGCCT CGAACAGGCA TCAAGCATCT CAAGCCTGAT 
GTCCCACCTA AACCCTCCGT AGTCTCACTC TCGACAAAAG TCAAGTCCAG TGACTCTGGC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023120175.1
CDS	409..3294
Translation	MRSEALLLYFTLLQIAGAGFPEDSEPISISHGNYTKQYPAFVGHKPGRNNTQRHKLDIQLIMIMNRTLYIAARDHIYTVDTDTANSDEIFFSKKMTWKSRQADVDTCRMKGKHKDECHNFIKVLLQQNDDTLFVCGTNAFNPSCRTYKMDTLEALGEEISGMARCPYDAKHANVALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDFIYFFFREIAMEYNTMGKVVFPRVARVCKNDRGGSPRVLEKQWTSFLKSRMNCSIPGDSHFYFNILQAVTDVIHINGRDVVMATFSTPYNSIPGSAVCAYDMAEVANTFTGRFKEQKSPDSTWTPFPEEKVPKPRPGNCAGTPSMERYKVSNEFPDDTLNFIKMHPLMDEAVPSIANRPWFLKTMVRYRLTRIVVDNEAGPNKNHTVVFLGSEKGIILKFLAKMNNGFLNDSLFLEELNVYNPDKCSIDGVEDKRIVGMQIDSKSHALWVAFTSCVVKVPLSRCERHGRCKKSCIASRDPYCGWVSEGACRQVVGNTKSAFEQDVEHGNTDGLGDCQNTFVALNGVIRESYQKGRDQLVPVTLLAIAVILAFAMGAIFSGIIVYCVCDHRRRDIDLTGRKEKDSHHLHSRRGSMNSVTKLTGLFETQAKDGRPEAILTPLMHNGRLTPNGKMLIKADQHHLDLAALPTPESTPMQPRRKPSRGSREWERNQNLINACTKDMPPMGSPVISTDLPLRASPGHIPSVVVLPLPQHQHQLQPHLQQHQQSYQHEYVEQPHHSDLSMGHGVMDDQGATLEYKTVKSPCHNLNMVDPDGVLPPRVPQREASLTIPPAVPQMGKRLEVHSSVYGLRKGSASAASGSSALKKHNTNSSNSSHLARHHSFNRVETPPPAPQRVDSIQMTAQGISLSRQPGMSSYGSLPRTGIKHLKPDVPPKPSVVSLSTKVKSSDSGT

Target ORF information:

RefSeq Version	XM_023264407.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris semaphorin 6A (sema6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023264407.1

ORF Insert Sequence:

ATGAGGTCAG AGGCCTTGTT ACTCTACTTC ACGCTACTGC AAATAGCCGG GGCGGGCTTC 
CCCGAAGACA GCGAACCTAT TAGCATCTCA CACGGCAACT ACACAAAGCA GTATCCAGCA 
TTTGTAGGCC ACAAACCTGG AAGGAACAAC ACGCAGCGGC ACAAACTGGA CATCCAGCTC 
ATCATGATCA TGAATAGGAC ATTATACATC GCTGCCAGGG ATCACATATA CACGGTGGAC 
ACGGACACTG CCAACAGTGA CGAGATCTTT TTCAGTAAGA AAATGACATG GAAGTCCAGA 
CAGGCAGATG TGGACACCTG CAGAATGAAA GGGAAGCATA AGGATGAGTG TCACAATTTC 
ATCAAAGTCC TCCTGCAGCA AAATGACGAC ACCTTGTTTG TTTGCGGAAC GAATGCTTTC 
AACCCTTCCT GTCGAACCTA CAAGATGGAC ACCCTGGAGG CTCTGGGAGA GGAGATCAGT 
GGGATGGCAC GCTGTCCGTA TGATGCCAAG CACGCCAACG TCGCCCTCTT TGCTGATGGC 
AAGCTGTACT CGGCCACCGT AACAGACTTT CTCGCGATCG ATGCGGTCAT CTATCGTAGC 
CTTGGAGACA GCCCGACTCT GCGCACTGTC AAACACGACT CCAAATGGCT GAAAGAGCCC 
TATTTCGTCC AGGCTGTCGA TTATGGGGAC TTCATCTACT TCTTCTTCCG CGAAATTGCT 
ATGGAGTACA ACACGATGGG AAAGGTGGTG TTTCCTCGGG TGGCAAGGGT TTGTAAGAAC 
GACCGAGGAG GCTCGCCTCG TGTCCTGGAG AAGCAGTGGA CCTCGTTTCT CAAGTCTCGG 
ATGAACTGCT CCATCCCCGG CGACTCCCAT TTCTACTTCA ACATCCTGCA GGCAGTAACG 
GACGTCATTC ACATCAATGG GAGAGATGTC GTCATGGCAA CTTTCTCCAC TCCTTACAAC 
AGTATTCCAG GTTCGGCTGT GTGTGCCTAT GACATGGCGG AGGTGGCCAA CACGTTCACG 
GGGCGTTTCA AAGAGCAGAA ATCCCCAGAC TCCACCTGGA CGCCTTTTCC CGAAGAGAAG 
GTTCCAAAGC CGAGGCCTGG GAACTGTGCC GGCACTCCAT CCATGGAGAG GTACAAGGTA 
TCCAATGAGT TCCCCGACGA CACACTCAAC TTCATCAAGA TGCATCCTCT GATGGATGAG 
GCCGTGCCCT CCATCGCTAA CAGGCCCTGG TTTCTCAAGA CCATGGTTCG GTATCGGCTG 
ACGCGCATCG TGGTGGACAA CGAGGCCGGT CCCAACAAGA ACCACACAGT GGTGTTCCTG 
GGATCTGAGA AAGGCATCAT CCTCAAGTTC TTGGCCAAAA TGAATAATGG TTTCCTCAAC 
GACAGCCTGT TTTTAGAGGA GCTCAACGTC TACAACCCTG ACAAGTGCAG TATAGATGGT 
GTGGAAGACA AGCGTATCGT CGGTATGCAG ATAGACAGCA AGAGCCATGC TCTGTGGGTC 
GCCTTCACTT CCTGTGTGGT CAAAGTGCCA CTGTCACGCT GTGAAAGGCA CGGACGATGC 
AAGAAGTCCT GTATAGCCTC CAGAGACCCG TACTGTGGCT GGGTATCAGA AGGGGCCTGC 
AGGCAGGTGG TTGGCAACAC CAAATCAGCC TTTGAGCAGG ACGTGGAGCA TGGTAACACA 
GATGGCCTGG GAGACTGCCA AAATACCTTT GTGGCTCTGA ACGGTGTGAT CCGTGAGAGC 
TACCAGAAGG GTCGAGACCA GCTCGTTCCT GTGACCCTGT TGGCCATCGC CGTTATCCTA 
GCGTTTGCGA TGGGTGCCAT CTTCTCTGGC ATCATCGTCT ACTGTGTCTG TGACCACCGG 
CGGCGAGACA TCGACCTGAC AGGCCGCAAG GAAAAGGACA GCCACCACCT ACACTCCCGC 
CGGGGATCCA TGAACAGTGT GACCAAGCTC ACCGGCCTGT TTGAGACCCA GGCCAAAGAC 
GGTCGCCCCG AGGCCATCCT CACCCCTTTG ATGCACAACG GGAGACTGAC ACCAAATGGG 
AAGATGCTGA TAAAAGCAGA CCAGCATCAC CTGGACCTTG CTGCTCTGCC AACGCCTGAA 
TCCACTCCCA TGCAGCCTCG TCGTAAGCCC AGCCGGGGCA GCCGTGAGTG GGAGAGAAAC 
CAGAACCTCA TCAACGCTTG CACCAAGGAC ATGCCACCTA TGGGCTCCCC TGTCATCTCT 
ACAGACCTGC CCCTGAGGGC CTCACCAGGA CACATCCCAA GCGTGGTAGT CCTACCTCTG 
CCGCAGCATC AGCACCAGCT CCAGCCTCAT CTGCAGCAGC ACCAGCAGTC CTACCAGCAT 
GAGTACGTGG AGCAGCCACA TCACAGCGAC CTGAGCATGG GCCACGGTGT CATGGATGAC 
CAGGGAGCCA CCCTGGAGTA CAAGACAGTG AAGAGCCCAT GTCACAACCT CAACATGGTG 
GATCCAGATG GGGTGCTGCC ACCTCGCGTG CCCCAACGAG AGGCCTCCCT TACAATCCCT 
CCTGCTGTCC CACAGATGGG CAAACGCCTG GAGGTCCACT CGTCGGTCTA CGGGCTCCGG 
AAAGGATCAG CGTCTGCTGC TTCTGGATCA TCCGCCCTAA AGAAGCACAA CACCAACTCA 
TCTAACTCCT CCCATTTGGC GAGACATCAC AGCTTCAACC GTGTGGAGAC TCCACCGCCT 
GCACCTCAGC GGGTGGACTC CATACAAATG ACAGCACAGG GCATATCTCT GTCCCGGCAG 
CCTGGAATGA GTTCCTACGG TTCACTGCCT CGAACAGGCA TCAAGCATCT CAAGCCTGAT 
GTCCCACCTA AACCCTCCGT AGTCTCACTC TCGACAAAAG TCAAGTCCAG TGACTCTGGC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema6a ( XM_023264406.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023120174.1 Amphiprion ocellaris semaphorin 6A (sema6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm103657
Clone ID Related Accession (Same CDS sequence)	XM_023264406.1
Accession Version	XM_023264406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	semaphorin-6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376180.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCAG AGGCCTTGTT ACTCTACTTC ACGCTACTGC AAATAGCCGG GGCGGGCTTC 
CCCGAAGACA GCGAACCTAT TAGCATCTCA CACGGCAACT ACACAAAGCA GTATCCAGCA 
TTTGTAGGCC ACAAACCTGG AAGGAACAAC ACGCAGCGGC ACAAACTGGA CATCCAGCTC 
ATCATGATCA TGAATAGGAC ATTATACATC GCTGCCAGGG ATCACATATA CACGGTGGAC 
ACGGACACTG CCAACAGTGA CGAGATCTTT TTCAGTAAGA AAATGACATG GAAGTCCAGA 
CAGGCAGATG TGGACACCTG CAGAATGAAA GGGAAGCATA AGGATGAGTG TCACAATTTC 
ATCAAAGTCC TCCTGCAGCA AAATGACGAC ACCTTGTTTG TTTGCGGAAC GAATGCTTTC 
AACCCTTCCT GTCGAACCTA CAAGATGGAC ACCCTGGAGG CTCTGGGAGA GGAGATCAGT 
GGGATGGCAC GCTGTCCGTA TGATGCCAAG CACGCCAACG TCGCCCTCTT TGCTGATGGC 
AAGCTGTACT CGGCCACCGT AACAGACTTT CTCGCGATCG ATGCGGTCAT CTATCGTAGC 
CTTGGAGACA GCCCGACTCT GCGCACTGTC AAACACGACT CCAAATGGCT GAAAGAGCCC 
TATTTCGTCC AGGCTGTCGA TTATGGGGAC TTCATCTACT TCTTCTTCCG CGAAATTGCT 
ATGGAGTACA ACACGATGGG AAAGGTGGTG TTTCCTCGGG TGGCAAGGGT TTGTAAGAAC 
GACCGAGGAG GCTCGCCTCG TGTCCTGGAG AAGCAGTGGA CCTCGTTTCT CAAGTCTCGG 
ATGAACTGCT CCATCCCCGG CGACTCCCAT TTCTACTTCA ACATCCTGCA GGCAGTAACG 
GACGTCATTC ACATCAATGG GAGAGATGTC GTCATGGCAA CTTTCTCCAC TCCTTACAAC 
AGTATTCCAG GTTCGGCTGT GTGTGCCTAT GACATGGCGG AGGTGGCCAA CACGTTCACG 
GGGCGTTTCA AAGAGCAGAA ATCCCCAGAC TCCACCTGGA CGCCTTTTCC CGAAGAGAAG 
GTTCCAAAGC CGAGGCCTGG GAACTGTGCC GGCACTCCAT CCATGGAGAG GTACAAGGTA 
TCCAATGAGT TCCCCGACGA CACACTCAAC TTCATCAAGA TGCATCCTCT GATGGATGAG 
GCCGTGCCCT CCATCGCTAA CAGGCCCTGG TTTCTCAAGA CCATGGTTCG GTATCGGCTG 
ACGCGCATCG TGGTGGACAA CGAGGCCGGT CCCAACAAGA ACCACACAGT GGTGTTCCTG 
GGATCTGAGA AAGGCATCAT CCTCAAGTTC TTGGCCAAAA TGAATAATGG TTTCCTCAAC 
GACAGCCTGT TTTTAGAGGA GCTCAACGTC TACAACCCTG ACAAGTGCAG TATAGATGGT 
GTGGAAGACA AGCGTATCGT CGGTATGCAG ATAGACAGCA AGAGCCATGC TCTGTGGGTC 
GCCTTCACTT CCTGTGTGGT CAAAGTGCCA CTGTCACGCT GTGAAAGGCA CGGACGATGC 
AAGAAGTCCT GTATAGCCTC CAGAGACCCG TACTGTGGCT GGGTATCAGA AGGGGCCTGC 
AGGCAGGTGG TTGGCAACAC CAAATCAGCC TTTGAGCAGG ACGTGGAGCA TGGTAACACA 
GATGGCCTGG GAGACTGCCA AAATACCTTT GTGGCTCTGA ACGGCCACCA TCACCACCAC 
CATCACCACA CCCTCTCACT TCCCACCTCG GCCCTCCCCG AGGCCGCGGA GGGGCCCCTA 
GGGCGGAAGG ACCCCCCCAT GAGTTCAGAC AAAGGGGAGG ACCCCTACGT GGCCGTGCCC 
TCTCAGACTC AGCCCGAAGC CAATGGTGTG ATCCGTGAGA GCTACCAGAA GGGTCGAGAC 
CAGCTCGTTC CTGTGACCCT GTTGGCCATC GCCGTTATCC TAGCGTTTGC GATGGGTGCC 
ATCTTCTCTG GCATCATCGT CTACTGTGTC TGTGACCACC GGCGGCGAGA CATCGACCTG 
ACAGGCCGCA AGGAAAAGGA CAGCCACCAC CTACACTCCC GCCGGGGATC CATGAACAGT 
GTGACCAAGC TCACCGGCCT GTTTGAGACC CAGGCCAAAG ACGGTCGCCC CGAGGCCATC 
CTCACCCCTT TGATGCACAA CGGGAGACTG ACACCAAATG GGAAGATGCT GATAAAAGCA 
GACCAGCATC ACCTGGACCT TGCTGCTCTG CCAACGCCTG AATCCACTCC CATGCAGCCT 
CGTCGTAAGC CCAGCCGGGG CAGCCGTGAG TGGGAGAGAA ACCAGAACCT CATCAACGCT 
TGCACCAAGG ACATGCCACC TATGGGCTCC CCTGTCATCT CTACAGACCT GCCCCTGAGG 
GCCTCACCAG GACACATCCC AAGCGTGGTA GTCCTACCTC TGCCGCAGCA TCAGCACCAG 
CTCCAGCCTC ATCTGCAGCA GCACCAGCAG TCCTACCAGC ATGAGTACGT GGAGCAGCCA 
CATCACAGCG ACCTGAGCAT GGGCCACGGT GTCATGGATG ACCAGGGAGC CACCCTGGAG 
TACAAGACAG TGAAGAGCCC ATGTCACAAC CTCAACATGG TGGATCCAGA TGGGGTGCTG 
CCACCTCGCG TGCCCCAACG AGAGGCCTCC CTTACAATCC CTCCTGCTGT CCCACAGATG 
GGCAAACGCC TGGAGGTCCA CTCGTCGGTC TACGGGCTCC GGAAAGGATC AGCGTCTGCT 
GCTTCTGGAT CATCCGCCCT AAAGAAGCAC AACACCAACT CATCTAACTC CTCCCATTTG 
GCGAGACATC ACAGCTTCAA CCGTGTGGAG ACTCCACCGC CTGCACCTCA GCGGGTGGAC 
TCCATACAAA TGACAGCACA GGGCATATCT CTGTCCCGGC AGCCTGGAAT GAGTTCCTAC 
GGTTCACTGC CTCGAACAGG CATCAAGCAT CTCAAGCCTG ATGTCCCACC TAAACCCTCC 
GTAGTCTCAC TCTCGACAAA AGTCAAGTCC AGTGACTCTG GCACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023120174.1
CDS	409..3456
Translation	MRSEALLLYFTLLQIAGAGFPEDSEPISISHGNYTKQYPAFVGHKPGRNNTQRHKLDIQLIMIMNRTLYIAARDHIYTVDTDTANSDEIFFSKKMTWKSRQADVDTCRMKGKHKDECHNFIKVLLQQNDDTLFVCGTNAFNPSCRTYKMDTLEALGEEISGMARCPYDAKHANVALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVDYGDFIYFFFREIAMEYNTMGKVVFPRVARVCKNDRGGSPRVLEKQWTSFLKSRMNCSIPGDSHFYFNILQAVTDVIHINGRDVVMATFSTPYNSIPGSAVCAYDMAEVANTFTGRFKEQKSPDSTWTPFPEEKVPKPRPGNCAGTPSMERYKVSNEFPDDTLNFIKMHPLMDEAVPSIANRPWFLKTMVRYRLTRIVVDNEAGPNKNHTVVFLGSEKGIILKFLAKMNNGFLNDSLFLEELNVYNPDKCSIDGVEDKRIVGMQIDSKSHALWVAFTSCVVKVPLSRCERHGRCKKSCIASRDPYCGWVSEGACRQVVGNTKSAFEQDVEHGNTDGLGDCQNTFVALNGHHHHHHHHTLSLPTSALPEAAEGPLGRKDPPMSSDKGEDPYVAVPSQTQPEANGVIRESYQKGRDQLVPVTLLAIAVILAFAMGAIFSGIIVYCVCDHRRRDIDLTGRKEKDSHHLHSRRGSMNSVTKLTGLFETQAKDGRPEAILTPLMHNGRLTPNGKMLIKADQHHLDLAALPTPESTPMQPRRKPSRGSREWERNQNLINACTKDMPPMGSPVISTDLPLRASPGHIPSVVVLPLPQHQHQLQPHLQQHQQSYQHEYVEQPHHSDLSMGHGVMDDQGATLEYKTVKSPCHNLNMVDPDGVLPPRVPQREASLTIPPAVPQMGKRLEVHSSVYGLRKGSASAASGSSALKKHNTNSSNSSHLARHHSFNRVETPPPAPQRVDSIQMTAQGISLSRQPGMSSYGSLPRTGIKHLKPDVPPKPSVVSLSTKVKSSDSGT

Target ORF information:

RefSeq Version	XM_023264406.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris semaphorin 6A (sema6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023264406.1

ORF Insert Sequence:

ATGAGGTCAG AGGCCTTGTT ACTCTACTTC ACGCTACTGC AAATAGCCGG GGCGGGCTTC 
CCCGAAGACA GCGAACCTAT TAGCATCTCA CACGGCAACT ACACAAAGCA GTATCCAGCA 
TTTGTAGGCC ACAAACCTGG AAGGAACAAC ACGCAGCGGC ACAAACTGGA CATCCAGCTC 
ATCATGATCA TGAATAGGAC ATTATACATC GCTGCCAGGG ATCACATATA CACGGTGGAC 
ACGGACACTG CCAACAGTGA CGAGATCTTT TTCAGTAAGA AAATGACATG GAAGTCCAGA 
CAGGCAGATG TGGACACCTG CAGAATGAAA GGGAAGCATA AGGATGAGTG TCACAATTTC 
ATCAAAGTCC TCCTGCAGCA AAATGACGAC ACCTTGTTTG TTTGCGGAAC GAATGCTTTC 
AACCCTTCCT GTCGAACCTA CAAGATGGAC ACCCTGGAGG CTCTGGGAGA GGAGATCAGT 
GGGATGGCAC GCTGTCCGTA TGATGCCAAG CACGCCAACG TCGCCCTCTT TGCTGATGGC 
AAGCTGTACT CGGCCACCGT AACAGACTTT CTCGCGATCG ATGCGGTCAT CTATCGTAGC 
CTTGGAGACA GCCCGACTCT GCGCACTGTC AAACACGACT CCAAATGGCT GAAAGAGCCC 
TATTTCGTCC AGGCTGTCGA TTATGGGGAC TTCATCTACT TCTTCTTCCG CGAAATTGCT 
ATGGAGTACA ACACGATGGG AAAGGTGGTG TTTCCTCGGG TGGCAAGGGT TTGTAAGAAC 
GACCGAGGAG GCTCGCCTCG TGTCCTGGAG AAGCAGTGGA CCTCGTTTCT CAAGTCTCGG 
ATGAACTGCT CCATCCCCGG CGACTCCCAT TTCTACTTCA ACATCCTGCA GGCAGTAACG 
GACGTCATTC ACATCAATGG GAGAGATGTC GTCATGGCAA CTTTCTCCAC TCCTTACAAC 
AGTATTCCAG GTTCGGCTGT GTGTGCCTAT GACATGGCGG AGGTGGCCAA CACGTTCACG 
GGGCGTTTCA AAGAGCAGAA ATCCCCAGAC TCCACCTGGA CGCCTTTTCC CGAAGAGAAG 
GTTCCAAAGC CGAGGCCTGG GAACTGTGCC GGCACTCCAT CCATGGAGAG GTACAAGGTA 
TCCAATGAGT TCCCCGACGA CACACTCAAC TTCATCAAGA TGCATCCTCT GATGGATGAG 
GCCGTGCCCT CCATCGCTAA CAGGCCCTGG TTTCTCAAGA CCATGGTTCG GTATCGGCTG 
ACGCGCATCG TGGTGGACAA CGAGGCCGGT CCCAACAAGA ACCACACAGT GGTGTTCCTG 
GGATCTGAGA AAGGCATCAT CCTCAAGTTC TTGGCCAAAA TGAATAATGG TTTCCTCAAC 
GACAGCCTGT TTTTAGAGGA GCTCAACGTC TACAACCCTG ACAAGTGCAG TATAGATGGT 
GTGGAAGACA AGCGTATCGT CGGTATGCAG ATAGACAGCA AGAGCCATGC TCTGTGGGTC 
GCCTTCACTT CCTGTGTGGT CAAAGTGCCA CTGTCACGCT GTGAAAGGCA CGGACGATGC 
AAGAAGTCCT GTATAGCCTC CAGAGACCCG TACTGTGGCT GGGTATCAGA AGGGGCCTGC 
AGGCAGGTGG TTGGCAACAC CAAATCAGCC TTTGAGCAGG ACGTGGAGCA TGGTAACACA 
GATGGCCTGG GAGACTGCCA AAATACCTTT GTGGCTCTGA ACGGCCACCA TCACCACCAC 
CATCACCACA CCCTCTCACT TCCCACCTCG GCCCTCCCCG AGGCCGCGGA GGGGCCCCTA 
GGGCGGAAGG ACCCCCCCAT GAGTTCAGAC AAAGGGGAGG ACCCCTACGT GGCCGTGCCC 
TCTCAGACTC AGCCCGAAGC CAATGGTGTG ATCCGTGAGA GCTACCAGAA GGGTCGAGAC 
CAGCTCGTTC CTGTGACCCT GTTGGCCATC GCCGTTATCC TAGCGTTTGC GATGGGTGCC 
ATCTTCTCTG GCATCATCGT CTACTGTGTC TGTGACCACC GGCGGCGAGA CATCGACCTG 
ACAGGCCGCA AGGAAAAGGA CAGCCACCAC CTACACTCCC GCCGGGGATC CATGAACAGT 
GTGACCAAGC TCACCGGCCT GTTTGAGACC CAGGCCAAAG ACGGTCGCCC CGAGGCCATC 
CTCACCCCTT TGATGCACAA CGGGAGACTG ACACCAAATG GGAAGATGCT GATAAAAGCA 
GACCAGCATC ACCTGGACCT TGCTGCTCTG CCAACGCCTG AATCCACTCC CATGCAGCCT 
CGTCGTAAGC CCAGCCGGGG CAGCCGTGAG TGGGAGAGAA ACCAGAACCT CATCAACGCT 
TGCACCAAGG ACATGCCACC TATGGGCTCC CCTGTCATCT CTACAGACCT GCCCCTGAGG 
GCCTCACCAG GACACATCCC AAGCGTGGTA GTCCTACCTC TGCCGCAGCA TCAGCACCAG 
CTCCAGCCTC ATCTGCAGCA GCACCAGCAG TCCTACCAGC ATGAGTACGT GGAGCAGCCA 
CATCACAGCG ACCTGAGCAT GGGCCACGGT GTCATGGATG ACCAGGGAGC CACCCTGGAG 
TACAAGACAG TGAAGAGCCC ATGTCACAAC CTCAACATGG TGGATCCAGA TGGGGTGCTG 
CCACCTCGCG TGCCCCAACG AGAGGCCTCC CTTACAATCC CTCCTGCTGT CCCACAGATG 
GGCAAACGCC TGGAGGTCCA CTCGTCGGTC TACGGGCTCC GGAAAGGATC AGCGTCTGCT 
GCTTCTGGAT CATCCGCCCT AAAGAAGCAC AACACCAACT CATCTAACTC CTCCCATTTG 
GCGAGACATC ACAGCTTCAA CCGTGTGGAG ACTCCACCGC CTGCACCTCA GCGGGTGGAC 
TCCATACAAA TGACAGCACA GGGCATATCT CTGTCCCGGC AGCCTGGAAT GAGTTCCTAC 
GGTTCACTGC CTCGAACAGG CATCAAGCAT CTCAAGCCTG ATGTCCCACC TAAACCCTCC 
GTAGTCTCAC TCTCGACAAA AGTCAAGTCC AGTGACTCTG GCACATAG