dock3 gene cDNA ORF clone, Fundulus heteroclitus(mummichog)

mRNA	Protein	Name
XM_012856652.1	XP_012712106.1	dedicator of cytokinesis protein 3 isoform X5
XM_021312993.1	XP_021168668.1	dedicator of cytokinesis protein 3 isoform X5
XM_012856649.1	XP_012712103.1	dedicator of cytokinesis protein 3 isoform X2
XM_012856654.1	XP_012712108.1	dedicator of cytokinesis protein 3 isoform X7
XM_021312995.1	XP_021168670.1	dedicator of cytokinesis protein 3 isoform X6
XM_021312996.1	XP_021168671.1	dedicator of cytokinesis protein 3 isoform X7
XM_012856651.1	XP_012712105.1	dedicator of cytokinesis protein 3 isoform X4
XM_012856653.1	XP_012712107.1	dedicator of cytokinesis protein 3 isoform X6
XM_012856655.1	XP_012712109.1	dedicator of cytokinesis protein 3 isoform X8
XM_012856656.1	XP_012712110.1	dedicator of cytokinesis protein 3 isoform X9
XM_021312991.1	XP_021168666.1	dedicator of cytokinesis protein 3 isoform X3
XM_021312997.1	XP_021168672.1	dedicator of cytokinesis protein 3 isoform X8
XM_012856648.1	XP_012712102.1	dedicator of cytokinesis protein 3 isoform X1
XM_012856650.1	XP_012712104.1	dedicator of cytokinesis protein 3 isoform X3
XM_012856652.2	XP_012712106.2	dedicator of cytokinesis protein 3 isoform X1
XM_021312990.1	XP_021168665.1	dedicator of cytokinesis protein 3 isoform X2
XM_021312992.1	XP_021168667.1	dedicator of cytokinesis protein 3 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu105384	XM_012856653.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFu105385	XM_012856654.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFu105386	XM_012856655.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFu105387	XM_012856656.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFu130686	XM_021312997.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFu105379	XM_012856648.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu105380	XM_012856649.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu105381	XM_012856650.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu105382	XM_012856651.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu105383	XM_012856652.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130679	XM_012856652.2 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130680	XM_021312990.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130681	XM_021312991.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130682	XM_021312992.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130683	XM_021312993.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130684	XM_021312995.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OFu130685	XM_021312996.1 Latest version!	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

dock3 ( XM_012856653.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712107.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OFu105384
Clone ID Related Accession (Same CDS sequence)	XM_012856653.1
Accession Version	XM_012856653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGCTTCTATG TTGCTTTTCA GGGTCCACCT 
GGTTTTGATA AGTCTGGCTC AGGAATCAAC AGAGGGATCC TGGCCTCTCA CAACCCGATG 
AGTCCTGAGG GCTTCAAGCT CATGCACCGA CACAGTCCCA TTGCTGTTTT GACTCCAGTG 
CGCATTTCCT CCCCTTCTCT TTCCTCTAAC TCCAGTGAAG CAGGAAATCT GCTGATCCTG 
GCGGATGGCA TGGTGGTGGA GCACCCAGAG GACTTCTATC GTATGCAGGC GAGCCCCTCC 
TCTTCAAGTC TTAGCTCCAC CCACTCTGCA CCTCCTCAGA TGATAAACTC CACCCCTCCC 
ACATTAAGAG TGGGCTCCCC TTCTCTGCCA GACAAGTACA GACACAATAG AGAAATGCTG 
ATGCTGCTGC CGCCCCACAG GGAGAGGCCG AGCAGTGCAA TGTACTCCAA CATCACAGAG 
AATGGACAGC CACCAAACTT TCAGAGAGCC TTGTTTCATC AGGTGATCGG TCCCTGTAAG 
CCCTGCAGTG ACCCCAACCT CTCTGTGGCT GAGAAAGTCC TCACCACTCC CAGCAGCTGG 
AGTTTGGACA GCGGCACCAG GGAGGCTCTG CCTTTCCTTT CTTCTCATGT TGGCAGCGTT 
ATGGCTCCAC CGGTTCCCCC GCGGAACGTG CCTCATGGGC AGCACCTGTC GATGCACTTT 
GACGCTTTCC ACCACCAGGT CAGCGACCTC CCTCCAGCTC TCCCTGCGCG CTCCTTAAGA 
AAGTCTCCCC TGCACCCTAT ACCTGCCTCG CCCACCAGTC CGCAGTCCGT CCTGGATGGC 
AGTAACTCCA CACTCTCAGG CAGTGCCAGC TCTGGGGTTT CATCCCTCAG TGAGAGCAAC 
TTTGGCGGCG CGACCTCCTC CTCAGACCCC GCGGCCAGCC GCACAGACAC CCTGGAGTCC 
GTCCCGAGCA GCCAGGCCTG GACCACCGAC CAGGAAGACT TGGACTCTCC TTATCAGCCC 
GTCAGGTACA GCATGTCTGA GCCAGACGTC CTGGAGGGAG TTAAATCCCC GCCTTGCCGC 
AGCAACTCTG CCCCAGGAGG AGTGAACCCG CCCCAGGTCA TCTATCAGAT CCAGTCTCAG 
GCTCCAGGTT TGGGCTTCAC CGGACCTCAG TCCCAACAGC CGGATGGACT GCAACAGCAA 
CAACACCCGT TTTACCACCA CCACTTCCAC CCACACTACA TTCCCCACAA TCCACCTCTC 
TATCATCTCC ATGAGCCCCC TCCTGCCGTA CCTCCTAAAC CCTACCTCAG AGAGGGCTGC 
ATCCCTGAGG AGGACCACCT GATCACCCAG TCTGGTCCAT CGCGCCCTCC CGTACCTCGA 
CCAATGCCGC GTAAGATCTC CCAGCCCTTA ATCGCAGCAA CCAAGGACGA ACAAGCTAAG 
GTGGCGTGGG AGCACGGCAT CAGTGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712107.1
CDS	35..6007
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856653.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856653.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGCTTCTATG TTGCTTTTCA GGGTCCACCT 
GGTTTTGATA AGTCTGGCTC AGGAATCAAC AGAGGGATCC TGGCCTCTCA CAACCCGATG 
AGTCCTGAGG GCTTCAAGCT CATGCACCGA CACAGTCCCA TTGCTGTTTT GACTCCAGTG 
CGCATTTCCT CCCCTTCTCT TTCCTCTAAC TCCAGTGAAG CAGGAAATCT GCTGATCCTG 
GCGGATGGCA TGGTGGTGGA GCACCCAGAG GACTTCTATC GTATGCAGGC GAGCCCCTCC 
TCTTCAAGTC TTAGCTCCAC CCACTCTGCA CCTCCTCAGA TGATAAACTC CACCCCTCCC 
ACATTAAGAG TGGGCTCCCC TTCTCTGCCA GACAAGTACA GACACAATAG AGAAATGCTG 
ATGCTGCTGC CGCCCCACAG GGAGAGGCCG AGCAGTGCAA TGTACTCCAA CATCACAGAG 
AATGGACAGC CACCAAACTT TCAGAGAGCC TTGTTTCATC AGGTGATCGG TCCCTGTAAG 
CCCTGCAGTG ACCCCAACCT CTCTGTGGCT GAGAAAGTCC TCACCACTCC CAGCAGCTGG 
AGTTTGGACA GCGGCACCAG GGAGGCTCTG CCTTTCCTTT CTTCTCATGT TGGCAGCGTT 
ATGGCTCCAC CGGTTCCCCC GCGGAACGTG CCTCATGGGC AGCACCTGTC GATGCACTTT 
GACGCTTTCC ACCACCAGGT CAGCGACCTC CCTCCAGCTC TCCCTGCGCG CTCCTTAAGA 
AAGTCTCCCC TGCACCCTAT ACCTGCCTCG CCCACCAGTC CGCAGTCCGT CCTGGATGGC 
AGTAACTCCA CACTCTCAGG CAGTGCCAGC TCTGGGGTTT CATCCCTCAG TGAGAGCAAC 
TTTGGCGGCG CGACCTCCTC CTCAGACCCC GCGGCCAGCC GCACAGACAC CCTGGAGTCC 
GTCCCGAGCA GCCAGGCCTG GACCACCGAC CAGGAAGACT TGGACTCTCC TTATCAGCCC 
GTCAGGTACA GCATGTCTGA GCCAGACGTC CTGGAGGGAG TTAAATCCCC GCCTTGCCGC 
AGCAACTCTG CCCCAGGAGG AGTGAACCCG CCCCAGGTCA TCTATCAGAT CCAGTCTCAG 
GCTCCAGGTT TGGGCTTCAC CGGACCTCAG TCCCAACAGC CGGATGGACT GCAACAGCAA 
CAACACCCGT TTTACCACCA CCACTTCCAC CCACACTACA TTCCCCACAA TCCACCTCTC 
TATCATCTCC ATGAGCCCCC TCCTGCCGTA CCTCCTAAAC CCTACCTCAG AGAGGGCTGC 
ATCCCTGAGG AGGACCACCT GATCACCCAG TCTGGTCCAT CGCGCCCTCC CGTACCTCGA 
CCAATGCCGC GTAAGATCTC CCAGCCCTTA ATCGCAGCAA CCAAGGACGA ACAAGCTAAG 
GTGGCGTGGG AGCACGGCAT CAGTGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856654.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712108.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X7, mRNA.

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CloneID	OFu105385
Clone ID Related Accession (Same CDS sequence)	XM_012856654.1
Accession Version	XM_012856654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGTCCACCTG GTTTTGATAA GTCTGGCTCA 
GGAATCAACA GAGGGATCCT GGCCTCTCAC AACCCGATGA GTCCTGAGGG CTTCAAGCTC 
ATGCACCGAC ACAGTCCCAT TGCTGTTTTG ACTCCAGTGC GCATTTCCTC CCCTTCTCTT 
TCCTCTAACT CCAGTGAAGC AGGAAATCTG CTGATCCTGG CGGATGGCAT GGTGGTGGAG 
CACCCAGAGG ACTTCTATCG TATGCAGGCG AGCCCCTCCT CTTCAAGTCT TAGCTCCACC 
CACTCTGCAC CTCCTCAGAT GATAAACTCC ACCCCTCCCA CATTAAGAGT GGGCTCCCCT 
TCTCTGCCAG ACAAGTACAG ACACAATAGA GAAATGCTGA TGCTGCTGCC GCCCCACAGG 
GAGAGGCCGA GCAGTGCAAT GTACTCCAAC ATCACAGAGA ATGGACAGCC ACCAAACTTT 
CAGAGAGCCT TGTTTCATCA GGTGATCGGT CCCTGTAAGC CCTGCAGTGA CCCCAACCTC 
TCTGTGGCTG AGAAAGTCCT CACCACTCCC AGCAGCTGGA GTTTGGACAG CGGCACCAGG 
GAGGCTCTGC CTTTCCTTTC TTCTCATGTT GGCAGCGTTA TGGCTCCACC GGTTCCCCCG 
CGGAACGTGC CTCATGGGCA GCACCTGTCG ATGCACTTTG ACGCTTTCCA CCACCAGGTC 
AGCGACCTCC CTCCAGCTCT CCCTGCGCGC TCCTTAAGAA AGTCTCCCCT GCACCCTATA 
CCTGCCTCGC CCACCAGTCC GCAGTCCGTC CTGGATGGCA GTAACTCCAC ACTCTCAGGC 
AGTGCCAGCT CTGGGGTTTC ATCCCTCAGT GAGAGCAACT TTGGCGGCGC GACCTCCTCC 
TCAGACCCCG CGGCCAGCCG CACAGACACC CTGGAGTCCG TCCCGAGCAG CCAGGCCTGG 
ACCACCGACC AGGAAGACTT GGACTCTCCT TATCAGCCCG TCAGGTACAG CATGTCTGAG 
CCAGACGTCC TGGAGGGAGT TAAATCCCCG CCTTGCCGCA GCAACTCTGC CCCAGGAGGA 
GTGAACCCGC CCCAGGTCAT CTATCAGATC CAGTCTCAGG CTCCAGGTTT GGGCTTCACC 
GGACCTCAGT CCCAACAGCC GGATGGACTG CAACAGCAAC AACACCCGTT TTACCACCAC 
CACTTCCACC CACACTACAT TCCCCACAAT CCACCTCTCT ATCATCTCCA TGAGCCCCCT 
CCTGCCGTAC CTCCTAAACC CTACCTCAGA GAGGGCTGCA TCCCTGAGGA GGACCACCTG 
ATCACCCAGT CTGGTCCATC GCGCCCTCCC GTACCTCGAC CAATGCCGCG TAAGATCTCC 
CAGCCCTTAA TCGCAGCAAC CAAGGACGAA CAAGCTAAGG TGGCGTGGGA GCACGGCATC 
AGTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712108.1
CDS	35..5986
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856654.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856654.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGTCCACCTG GTTTTGATAA GTCTGGCTCA 
GGAATCAACA GAGGGATCCT GGCCTCTCAC AACCCGATGA GTCCTGAGGG CTTCAAGCTC 
ATGCACCGAC ACAGTCCCAT TGCTGTTTTG ACTCCAGTGC GCATTTCCTC CCCTTCTCTT 
TCCTCTAACT CCAGTGAAGC AGGAAATCTG CTGATCCTGG CGGATGGCAT GGTGGTGGAG 
CACCCAGAGG ACTTCTATCG TATGCAGGCG AGCCCCTCCT CTTCAAGTCT TAGCTCCACC 
CACTCTGCAC CTCCTCAGAT GATAAACTCC ACCCCTCCCA CATTAAGAGT GGGCTCCCCT 
TCTCTGCCAG ACAAGTACAG ACACAATAGA GAAATGCTGA TGCTGCTGCC GCCCCACAGG 
GAGAGGCCGA GCAGTGCAAT GTACTCCAAC ATCACAGAGA ATGGACAGCC ACCAAACTTT 
CAGAGAGCCT TGTTTCATCA GGTGATCGGT CCCTGTAAGC CCTGCAGTGA CCCCAACCTC 
TCTGTGGCTG AGAAAGTCCT CACCACTCCC AGCAGCTGGA GTTTGGACAG CGGCACCAGG 
GAGGCTCTGC CTTTCCTTTC TTCTCATGTT GGCAGCGTTA TGGCTCCACC GGTTCCCCCG 
CGGAACGTGC CTCATGGGCA GCACCTGTCG ATGCACTTTG ACGCTTTCCA CCACCAGGTC 
AGCGACCTCC CTCCAGCTCT CCCTGCGCGC TCCTTAAGAA AGTCTCCCCT GCACCCTATA 
CCTGCCTCGC CCACCAGTCC GCAGTCCGTC CTGGATGGCA GTAACTCCAC ACTCTCAGGC 
AGTGCCAGCT CTGGGGTTTC ATCCCTCAGT GAGAGCAACT TTGGCGGCGC GACCTCCTCC 
TCAGACCCCG CGGCCAGCCG CACAGACACC CTGGAGTCCG TCCCGAGCAG CCAGGCCTGG 
ACCACCGACC AGGAAGACTT GGACTCTCCT TATCAGCCCG TCAGGTACAG CATGTCTGAG 
CCAGACGTCC TGGAGGGAGT TAAATCCCCG CCTTGCCGCA GCAACTCTGC CCCAGGAGGA 
GTGAACCCGC CCCAGGTCAT CTATCAGATC CAGTCTCAGG CTCCAGGTTT GGGCTTCACC 
GGACCTCAGT CCCAACAGCC GGATGGACTG CAACAGCAAC AACACCCGTT TTACCACCAC 
CACTTCCACC CACACTACAT TCCCCACAAT CCACCTCTCT ATCATCTCCA TGAGCCCCCT 
CCTGCCGTAC CTCCTAAACC CTACCTCAGA GAGGGCTGCA TCCCTGAGGA GGACCACCTG 
ATCACCCAGT CTGGTCCATC GCGCCCTCCC GTACCTCGAC CAATGCCGCG TAAGATCTCC 
CAGCCCTTAA TCGCAGCAAC CAAGGACGAA CAAGCTAAGG TGGCGTGGGA GCACGGCATC 
AGTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856655.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712109.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X8, mRNA.

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CloneID	OFu105386
Clone ID Related Accession (Same CDS sequence)	XM_012856655.1
Accession Version	XM_012856655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTTCTGTT GGAGAAGATT GAGCAGGAGA CGTGGAAAGA AACAGGAATC 
TCGTTTGTTA CATCGGTTAC ACGGTTGATG GAGCGACTGC TGGACTACAG GGACTGTATG 
AAGGGAGATG AGACAGAAAA CAAGAAGATT GGCTGCACAG TGAACCTTCT TAATTTCTAC 
AAATCGGAAA TCAACAAAGA GGAGATGTAC ATCCGTTACA TCCACAAATT GTGTGACATG 
CATCTGCAGG CTGAGAACTA CACTGAGGCT GCATTCACGC TGCTGTTGTA CTGGGAGCTG 
CTGCACTGGG ACGAGCGGCC AGTTAAAGAG TTCCTGCATT ATCCTGCTCA GACTGAGTGG 
CACCGGAAGG AGGGCCTCTG CCGCAAAGTC ATCCACTATT TCAACAAGGG AAAGTGTTGG 
GAATTTGGCA TTCCTCTGTG CAGAGAACTG GCCTTCCAGT ATGAATCTCT CTACGACTAC 
CAGAGTCTCA GCTGGATACG GAAAATGGAG GCAGCATATT ATGACAATAT CATGGAGCAG 
CAGCGTCTGG AGCCGGAGTT CTTCAGGGTG GGATTCTACG GCAGGAAGTT TCCTTTCTTC 
CTCAGAAACA AAGAGTTTGT CTGCCGGGGC CATGACTATG AAAGGTTAGA AGCCTTCCAA 
CAAAGGATGC TGGGAGAATT CCCACAAGCC ATTGCAATGC AGCACCCGAA TCAGCCAGAT 
GAGGCCATTC TGCAGTGTGA TGCACAGTAC CTCCAGATTT ACGCTGTGAC ACCGTTTCCA 
GAAAGCGTCA CCGTCCTTCA GATGGACAGA GTTCCAGACC GCATCAAGAG CTTCTATCGA 
GTGAACAACG TTCGGCGATT TCGTTACGAC CGGCCGTTCC ACAAAGGACC CAAAGACAGA 
GAGAATGAGT TTAAGAGTCT CTGGATAGAA AGGACGACTC TGATCCTCAC CCATCCTCTG 
CCTGGAATTT CATGCTGGTT TGAGGTAGAA AAGAGGGAAC TGGTGGAGGT GAGTCCATTG 
GAAAACGCCA TCTCTGTGGT GGAAAATAAG AACCAGGAGC TGCGGACTCT GATCAGTCAG 
TACCAACACA AGCAGCTGCA CGGGAACATC AACCTGCTCA GCATGACGCT GAATGGAGTC 
ATCGACGCCG CCGTGAACGG GGGCATCGCC AGATACCAGG AGGCTTTCTT TGATAAGGAA 
TACATCACTA GTCACCCTGA AGACACAGAG AAGATCACGC AGCTCAAAGA TCTCATGCAG 
GAGCAGGTTC ATATCTTAGG GGTGGGCTTG GCTGTGCACG AGAAGTTGGT GCACCCAGAA 
ATGCGTCCCC TGCACAAGAA GCTGATCGAT CAGTTCCAGA TGATGAGGAG CAGCCTCTGC 
CATGGCTTCT ATGTTGCTTT TCAGGGTCCA CCTGGTTTTG ATAAGTCTGG CTCAGGAATC 
AACAGAGGGA TCCTGGCCTC TCACAACCCG ATGAGTCCTG AGGGCTTCAA GCTCATGCAC 
CGACACAGTC CCATTGCTGT TTTGACTCCA GTGCGCATTT CCTCCCCTTC TCTTTCCTCT 
AACTCCAGTG AAGCAGGAAA TCTGCTGATC CTGGCGGATG GCATGGTGGT GGAGCACCCA 
GAGGACTTCT ATCGTATGCA GGCGAGCCCC TCCTCTTCAA GTCTTAGCTC CACCCACTCT 
GCACCTCCTC AGATGATAAA CTCCACCCCT CCCACATTAA GAGTGGGCTC CCCTTCTCTG 
CCAGACAAGT ACAGACACAA TAGAGAAATG CTGATGCTGC TGCCGCCCCA CAGGGAGAGG 
CCGAGCAGTG CAATGTACTC CAACATCACA GAGAATGGAC AGCCACCAAA CTTTCAGAGA 
GCCTTGTTTC ATCAGGTGAT CGGTCCCTGT AAGCCCTGCA GTGACCCCAA CCTCTCTGTG 
GCTGAGAAAG TCCTCACCAC TCCCAGCAGC TGGAGTTTGG ACAGCGGCAC CAGGGAGGCT 
CTGCCTTTCC TTTCTTCTCA TGTTGGCAGC GTTATGGCTC CACCGGTTCC CCCGCGGAAC 
GTGCCTCATG GGCAGCACCT GTCGATGCAC TTTGACGCTT TCCACCACCA GGTCAGCGAC 
CTCCCTCCAG CTCTCCCTGC GCGCTCCTTA AGAAAGTCTC CCCTGCACCC TATACCTGCC 
TCGCCCACCA GTCCGCAGTC CGTCCTGGAT GGCAGTAACT CCACACTCTC AGGCAGTGCC 
AGCTCTGGGG TTTCATCCCT CAGTGAGAGC AACTTTGGCG GCGCGACCTC CTCCTCAGAC 
CCCGCGGCCA GCCGCACAGA CACCCTGGAG TCCGTCCCGA GCAGCCAGGC CTGGACCACC 
GACCAGGAAG ACTTGGACTC TCCTTATCAG CCCGTCAGGT ACAGCATGTC TGAGCCAGAC 
GTCCTGGAGG GAGTTAAATC CCCGCCTTGC CGCAGCAACT CTGCCCCAGG AGGAGTGAAC 
CCGCCCCAGG TCATCTATCA GATCCAGTCT CAGGCTCCAG GTTTGGGCTT CACCGGACCT 
CAGTCCCAAC AGCCGGATGG ACTGCAACAG CAACAACACC CGTTTTACCA CCACCACTTC 
CACCCACACT ACATTCCCCA CAATCCACCT CTCTATCATC TCCATGAGCC CCCTCCTGCC 
GTACCTCCTA AACCCTACCT CAGAGAGGGC TGCATCCCTG AGGAGGACCA CCTGATCACC 
CAGTCTGGTC CATCGCGCCC TCCCGTACCT CGACCAATGC CGCGTAAGAT CTCCCAGCCC 
TTAATCGCAG CAACCAAGGA CGAACAAGCT AAGGTGGCGT GGGAGCACGG CATCAGTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712109.1
CDS	35..5980
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856655.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856655.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTTCTGTT GGAGAAGATT GAGCAGGAGA CGTGGAAAGA AACAGGAATC 
TCGTTTGTTA CATCGGTTAC ACGGTTGATG GAGCGACTGC TGGACTACAG GGACTGTATG 
AAGGGAGATG AGACAGAAAA CAAGAAGATT GGCTGCACAG TGAACCTTCT TAATTTCTAC 
AAATCGGAAA TCAACAAAGA GGAGATGTAC ATCCGTTACA TCCACAAATT GTGTGACATG 
CATCTGCAGG CTGAGAACTA CACTGAGGCT GCATTCACGC TGCTGTTGTA CTGGGAGCTG 
CTGCACTGGG ACGAGCGGCC AGTTAAAGAG TTCCTGCATT ATCCTGCTCA GACTGAGTGG 
CACCGGAAGG AGGGCCTCTG CCGCAAAGTC ATCCACTATT TCAACAAGGG AAAGTGTTGG 
GAATTTGGCA TTCCTCTGTG CAGAGAACTG GCCTTCCAGT ATGAATCTCT CTACGACTAC 
CAGAGTCTCA GCTGGATACG GAAAATGGAG GCAGCATATT ATGACAATAT CATGGAGCAG 
CAGCGTCTGG AGCCGGAGTT CTTCAGGGTG GGATTCTACG GCAGGAAGTT TCCTTTCTTC 
CTCAGAAACA AAGAGTTTGT CTGCCGGGGC CATGACTATG AAAGGTTAGA AGCCTTCCAA 
CAAAGGATGC TGGGAGAATT CCCACAAGCC ATTGCAATGC AGCACCCGAA TCAGCCAGAT 
GAGGCCATTC TGCAGTGTGA TGCACAGTAC CTCCAGATTT ACGCTGTGAC ACCGTTTCCA 
GAAAGCGTCA CCGTCCTTCA GATGGACAGA GTTCCAGACC GCATCAAGAG CTTCTATCGA 
GTGAACAACG TTCGGCGATT TCGTTACGAC CGGCCGTTCC ACAAAGGACC CAAAGACAGA 
GAGAATGAGT TTAAGAGTCT CTGGATAGAA AGGACGACTC TGATCCTCAC CCATCCTCTG 
CCTGGAATTT CATGCTGGTT TGAGGTAGAA AAGAGGGAAC TGGTGGAGGT GAGTCCATTG 
GAAAACGCCA TCTCTGTGGT GGAAAATAAG AACCAGGAGC TGCGGACTCT GATCAGTCAG 
TACCAACACA AGCAGCTGCA CGGGAACATC AACCTGCTCA GCATGACGCT GAATGGAGTC 
ATCGACGCCG CCGTGAACGG GGGCATCGCC AGATACCAGG AGGCTTTCTT TGATAAGGAA 
TACATCACTA GTCACCCTGA AGACACAGAG AAGATCACGC AGCTCAAAGA TCTCATGCAG 
GAGCAGGTTC ATATCTTAGG GGTGGGCTTG GCTGTGCACG AGAAGTTGGT GCACCCAGAA 
ATGCGTCCCC TGCACAAGAA GCTGATCGAT CAGTTCCAGA TGATGAGGAG CAGCCTCTGC 
CATGGCTTCT ATGTTGCTTT TCAGGGTCCA CCTGGTTTTG ATAAGTCTGG CTCAGGAATC 
AACAGAGGGA TCCTGGCCTC TCACAACCCG ATGAGTCCTG AGGGCTTCAA GCTCATGCAC 
CGACACAGTC CCATTGCTGT TTTGACTCCA GTGCGCATTT CCTCCCCTTC TCTTTCCTCT 
AACTCCAGTG AAGCAGGAAA TCTGCTGATC CTGGCGGATG GCATGGTGGT GGAGCACCCA 
GAGGACTTCT ATCGTATGCA GGCGAGCCCC TCCTCTTCAA GTCTTAGCTC CACCCACTCT 
GCACCTCCTC AGATGATAAA CTCCACCCCT CCCACATTAA GAGTGGGCTC CCCTTCTCTG 
CCAGACAAGT ACAGACACAA TAGAGAAATG CTGATGCTGC TGCCGCCCCA CAGGGAGAGG 
CCGAGCAGTG CAATGTACTC CAACATCACA GAGAATGGAC AGCCACCAAA CTTTCAGAGA 
GCCTTGTTTC ATCAGGTGAT CGGTCCCTGT AAGCCCTGCA GTGACCCCAA CCTCTCTGTG 
GCTGAGAAAG TCCTCACCAC TCCCAGCAGC TGGAGTTTGG ACAGCGGCAC CAGGGAGGCT 
CTGCCTTTCC TTTCTTCTCA TGTTGGCAGC GTTATGGCTC CACCGGTTCC CCCGCGGAAC 
GTGCCTCATG GGCAGCACCT GTCGATGCAC TTTGACGCTT TCCACCACCA GGTCAGCGAC 
CTCCCTCCAG CTCTCCCTGC GCGCTCCTTA AGAAAGTCTC CCCTGCACCC TATACCTGCC 
TCGCCCACCA GTCCGCAGTC CGTCCTGGAT GGCAGTAACT CCACACTCTC AGGCAGTGCC 
AGCTCTGGGG TTTCATCCCT CAGTGAGAGC AACTTTGGCG GCGCGACCTC CTCCTCAGAC 
CCCGCGGCCA GCCGCACAGA CACCCTGGAG TCCGTCCCGA GCAGCCAGGC CTGGACCACC 
GACCAGGAAG ACTTGGACTC TCCTTATCAG CCCGTCAGGT ACAGCATGTC TGAGCCAGAC 
GTCCTGGAGG GAGTTAAATC CCCGCCTTGC CGCAGCAACT CTGCCCCAGG AGGAGTGAAC 
CCGCCCCAGG TCATCTATCA GATCCAGTCT CAGGCTCCAG GTTTGGGCTT CACCGGACCT 
CAGTCCCAAC AGCCGGATGG ACTGCAACAG CAACAACACC CGTTTTACCA CCACCACTTC 
CACCCACACT ACATTCCCCA CAATCCACCT CTCTATCATC TCCATGAGCC CCCTCCTGCC 
GTACCTCCTA AACCCTACCT CAGAGAGGGC TGCATCCCTG AGGAGGACCA CCTGATCACC 
CAGTCTGGTC CATCGCGCCC TCCCGTACCT CGACCAATGC CGCGTAAGAT CTCCCAGCCC 
TTAATCGCAG CAACCAAGGA CGAACAAGCT AAGGTGGCGT GGGAGCACGG CATCAGTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856656.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712110.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X9, mRNA.

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CloneID	OFu105387
Clone ID Related Accession (Same CDS sequence)	XM_012856656.1
Accession Version	XM_012856656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGCT TCTATGTTGC TTTTCAGGGT 
CCACCTGGTT TTGATAAGTC TGGCTCAGGA ATCAACAGAG GGATCCTGGC CTCTCACAAC 
CCGATGAGTC CTGAGGGCTT CAAGCTCATG CACCGACACA GTCCCATTGC TGTTTTGACT 
CCAGTGCGCA TTTCCTCCCC TTCTCTTTCC TCTAACTCCA GTGAAGCAGG AAATCTGCTG 
ATCCTGGCGG ATGGCATGGT GGTGGAGCAC CCAGAGGACT TCTATCGTAT GCAGGCGAGC 
CCCTCCTCTT CAAGTCTTAG CTCCACCCAC TCTGCACCTC CTCAGATGAT AAACTCCACC 
CCTCCCACAT TAAGAGTGGG CTCCCCTTCT CTGCCAGACA AGTACAGACA CAATAGAGAA 
ATGCTGATGC TGCTGCCGCC CCACAGGGAG AGGCCGAGCA GTGCAATGTA CTCCAACATC 
ACAGAGAATG GACAGCCACC AAACTTTCAG AGAGCCTTGT TTCATCAGGT GATCGGTCCC 
TGTAAGCCCT GCAGTGACCC CAACCTCTCT GTGGCTGAGA AAGTCCTCAC CACTCCCAGC 
AGCTGGAGTT TGGACAGCGG CACCAGGGAG GCTCTGCCTT TCCTTTCTTC TCATGTTGGC 
AGCGTTATGG CTCCACCGGT TCCCCCGCGG AACGTGCCTC ATGTCTCCCC TGCACCCTAT 
ACCTGCCTCG CCCACCAGTC CGCAGTCCGT CCTGGATGGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712110.1
CDS	35..5479
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHVSPAPYTCLAHQSAVRPGWQ

Target ORF information:

RefSeq Version	XM_012856656.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856656.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGCT TCTATGTTGC TTTTCAGGGT 
CCACCTGGTT TTGATAAGTC TGGCTCAGGA ATCAACAGAG GGATCCTGGC CTCTCACAAC 
CCGATGAGTC CTGAGGGCTT CAAGCTCATG CACCGACACA GTCCCATTGC TGTTTTGACT 
CCAGTGCGCA TTTCCTCCCC TTCTCTTTCC TCTAACTCCA GTGAAGCAGG AAATCTGCTG 
ATCCTGGCGG ATGGCATGGT GGTGGAGCAC CCAGAGGACT TCTATCGTAT GCAGGCGAGC 
CCCTCCTCTT CAAGTCTTAG CTCCACCCAC TCTGCACCTC CTCAGATGAT AAACTCCACC 
CCTCCCACAT TAAGAGTGGG CTCCCCTTCT CTGCCAGACA AGTACAGACA CAATAGAGAA 
ATGCTGATGC TGCTGCCGCC CCACAGGGAG AGGCCGAGCA GTGCAATGTA CTCCAACATC 
ACAGAGAATG GACAGCCACC AAACTTTCAG AGAGCCTTGT TTCATCAGGT GATCGGTCCC 
TGTAAGCCCT GCAGTGACCC CAACCTCTCT GTGGCTGAGA AAGTCCTCAC CACTCCCAGC 
AGCTGGAGTT TGGACAGCGG CACCAGGGAG GCTCTGCCTT TCCTTTCTTC TCATGTTGGC 
AGCGTTATGG CTCCACCGGT TCCCCCGCGG AACGTGCCTC ATGTCTCCCC TGCACCCTAT 
ACCTGCCTCG CCCACCAGTC CGCAGTCCGT CCTGGATGGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312997.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168672.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X8, mRNA.

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CloneID	OFu130686
Clone ID Related Accession (Same CDS sequence)	XM_021312997.1
Accession Version	XM_021312997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGCTTCTATG TTGCTTTTCA GGGTCCACCT 
GGTTTTGATA AGTCTGGCTC AGGAATCAAC AGAGGGATCC TGGCCTCTCA CAACCCGATG 
AGTCCTGAGG GCTTCAAGCT CATGCACCGA CACAGTGAAG CAGGAAATCT GCTGATCCTG 
GCGGATGGCA TGGTGGTGGA GCACCCAGAG GACTTCTATC GTATGCAGGC GAGCCCCTCC 
TCTTCAAGTC TTAGCTCCAC CCACTCTGCA CCTCCTCAGA TGATAAACTC CACCCCTCCC 
ACATTAAGAG TGGGCTCCCC TTCTCTGCCA GACAAGTACA GACACAATAG AGAAATGCTG 
ATGCTGCTGC CGCCCCACAG GGAGAGGCCG AGCAGTGCAA TGTACTCCAA CATCACAGAG 
AATGGACAGC CACCAAACTT TCAGAGAGCC TTGTTTCATC AGGTGATCGG TCCCTGTAAG 
CCCTGCAGTG ACCCCAACCT CTCTGTGGCT GAGAAAGTCC TCACCACTCC CAGCAGCTGG 
AGTTTGGACA GCGGCACCAG GGAGGCTCTG CCTTTCCTTT CTTCTCATGT TGGCAGCGTT 
ATGGCTCCAC CGGTTCCCCC GCGGAACGTG CCTCATGTCT CCCCTGCACC CTATACCTGC 
CTCGCCCACC AGTCCGCAGT CCGTCCTGGA TGGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168672.1
CDS	185..5923
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHVSPAPYTCLAHQSAVRPGWQ

Target ORF information:

RefSeq Version	XM_021312997.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312997.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGCTTCTATG TTGCTTTTCA GGGTCCACCT 
GGTTTTGATA AGTCTGGCTC AGGAATCAAC AGAGGGATCC TGGCCTCTCA CAACCCGATG 
AGTCCTGAGG GCTTCAAGCT CATGCACCGA CACAGTGAAG CAGGAAATCT GCTGATCCTG 
GCGGATGGCA TGGTGGTGGA GCACCCAGAG GACTTCTATC GTATGCAGGC GAGCCCCTCC 
TCTTCAAGTC TTAGCTCCAC CCACTCTGCA CCTCCTCAGA TGATAAACTC CACCCCTCCC 
ACATTAAGAG TGGGCTCCCC TTCTCTGCCA GACAAGTACA GACACAATAG AGAAATGCTG 
ATGCTGCTGC CGCCCCACAG GGAGAGGCCG AGCAGTGCAA TGTACTCCAA CATCACAGAG 
AATGGACAGC CACCAAACTT TCAGAGAGCC TTGTTTCATC AGGTGATCGG TCCCTGTAAG 
CCCTGCAGTG ACCCCAACCT CTCTGTGGCT GAGAAAGTCC TCACCACTCC CAGCAGCTGG 
AGTTTGGACA GCGGCACCAG GGAGGCTCTG CCTTTCCTTT CTTCTCATGT TGGCAGCGTT 
ATGGCTCCAC CGGTTCCCCC GCGGAACGTG CCTCATGTCT CCCCTGCACC CTATACCTGC 
CTCGCCCACC AGTCCGCAGT CCGTCCTGGA TGGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856648.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712102.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X1, mRNA.

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CloneID	OFu105379
Clone ID Related Accession (Same CDS sequence)	XM_012856648.1
Accession Version	XM_012856648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGCT TCTATGTTGC TTTTCAGGGT 
CCACCTGGTT TTGATAAGTC TGGCTCAGGA ATCAACAGAG GGATCCTGGC CTCTCACAAC 
CCGATGAGTC CTGAGGGCTT CAAGCTCATG CACCGACACA GTCCCATTGC TGTTTTGACT 
CCAGTGCGCA TTTCCTCCCC TTCTCTTTCC TCTAACTCCA GTGAAGCAGG AAATCTGCTG 
ATCCTGGCGG ATGGCATGGT GGTGGAGCAC CCAGAGGACT TCTATCGTAT GCAGGCGAGC 
CCCTCCTCTT CAAGTCTTAG CTCCACCCAC TCTGCACCTC CTCAGATGAT AAACTCCACC 
CCTCCCACAT TAAGAGTGGG CTCCCCTTCT CTGCCAGACA AGTACAGACA CAATAGAGAA 
ATGCTGATGC TGCTGCCGCC CCACAGGGAG AGGCCGAGCA GTGCAATGTA CTCCAACATC 
ACAGAGAATG GACAGCCACC AAACTTTCAG AGAGCCTTGT TTCATCAGGT GATCGGTCCC 
TGTAAGCCCT GCAGTGACCC CAACCTCTCT GTGGCTGAGA AAGTCCTCAC CACTCCCAGC 
AGCTGGAGTT TGGACAGCGG CACCAGGGAG GCTCTGCCTT TCCTTTCTTC TCATGTTGGC 
AGCGTTATGG CTCCACCGGT TCCCCCGCGG AACGTGCCTC ATGGGCAGCA CCTGTCGATG 
CACTTTGACG CTTTCCACCA CCAGGTCAGC GACCTCCCTC CAGCTCTCCC TGCGCGCTCC 
TTAAGAAAGT CTCCCCTGCA CCCTATACCT GCCTCGCCCA CCAGTCCGCA GTCCGTCCTG 
GATGGCAGTA ACTCCACACT CTCAGGCAGT GCCAGCTCTG GGGTTTCATC CCTCAGTGAG 
AGCAACTTTG GCGGCGCGAC CTCCTCCTCA GACCCCGCGG CCAGCCGCAC AGACACCCTG 
GAGTCCGTCC CGAGCAGCCA GGCCTGGACC ACCGACCAGG AAGACTTGGA CTCTCCTTAT 
CAGCCCGTCA GGTACAGCAT GTCTGAGCCA GACGTCCTGG AGGGAGTTAA ATCCCCGCCT 
TGCCGCAGCA ACTCTGCCCC AGGAGGAGTG AACCCGCCCC AGGTCATCTA TCAGATCCAG 
TCTCAGGCTC CAGGTTTGGG CTTCACCGGA CCTCAGTCCC AACAGCCGGA TGGACTGCAA 
CAGCAACAAC ACCCGTTTTA CCACCACCAC TTCCACCCAC ACTACATTCC CCACAATCCA 
CCTCTCTATC ATCTCCATGA GCCCCCTCCT GCCGTACCTC CTAAACCCTA CCTCAGAGAG 
GGCTGCATCC CTGAGGAGGA CCACCTGATC ACCCAGTCTG GTCCATCGCG CCCTCCCGTA 
CCTCGACCAA TGCCGCGTAA GATCTCCCAG CCCTTAATCG CAGCAACCAA GGACGAACAA 
GCTAAGGTGG CGTGGGAGCA CGGCATCAGT GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712102.1
CDS	35..6193
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856648.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856648.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGCT TCTATGTTGC TTTTCAGGGT 
CCACCTGGTT TTGATAAGTC TGGCTCAGGA ATCAACAGAG GGATCCTGGC CTCTCACAAC 
CCGATGAGTC CTGAGGGCTT CAAGCTCATG CACCGACACA GTCCCATTGC TGTTTTGACT 
CCAGTGCGCA TTTCCTCCCC TTCTCTTTCC TCTAACTCCA GTGAAGCAGG AAATCTGCTG 
ATCCTGGCGG ATGGCATGGT GGTGGAGCAC CCAGAGGACT TCTATCGTAT GCAGGCGAGC 
CCCTCCTCTT CAAGTCTTAG CTCCACCCAC TCTGCACCTC CTCAGATGAT AAACTCCACC 
CCTCCCACAT TAAGAGTGGG CTCCCCTTCT CTGCCAGACA AGTACAGACA CAATAGAGAA 
ATGCTGATGC TGCTGCCGCC CCACAGGGAG AGGCCGAGCA GTGCAATGTA CTCCAACATC 
ACAGAGAATG GACAGCCACC AAACTTTCAG AGAGCCTTGT TTCATCAGGT GATCGGTCCC 
TGTAAGCCCT GCAGTGACCC CAACCTCTCT GTGGCTGAGA AAGTCCTCAC CACTCCCAGC 
AGCTGGAGTT TGGACAGCGG CACCAGGGAG GCTCTGCCTT TCCTTTCTTC TCATGTTGGC 
AGCGTTATGG CTCCACCGGT TCCCCCGCGG AACGTGCCTC ATGGGCAGCA CCTGTCGATG 
CACTTTGACG CTTTCCACCA CCAGGTCAGC GACCTCCCTC CAGCTCTCCC TGCGCGCTCC 
TTAAGAAAGT CTCCCCTGCA CCCTATACCT GCCTCGCCCA CCAGTCCGCA GTCCGTCCTG 
GATGGCAGTA ACTCCACACT CTCAGGCAGT GCCAGCTCTG GGGTTTCATC CCTCAGTGAG 
AGCAACTTTG GCGGCGCGAC CTCCTCCTCA GACCCCGCGG CCAGCCGCAC AGACACCCTG 
GAGTCCGTCC CGAGCAGCCA GGCCTGGACC ACCGACCAGG AAGACTTGGA CTCTCCTTAT 
CAGCCCGTCA GGTACAGCAT GTCTGAGCCA GACGTCCTGG AGGGAGTTAA ATCCCCGCCT 
TGCCGCAGCA ACTCTGCCCC AGGAGGAGTG AACCCGCCCC AGGTCATCTA TCAGATCCAG 
TCTCAGGCTC CAGGTTTGGG CTTCACCGGA CCTCAGTCCC AACAGCCGGA TGGACTGCAA 
CAGCAACAAC ACCCGTTTTA CCACCACCAC TTCCACCCAC ACTACATTCC CCACAATCCA 
CCTCTCTATC ATCTCCATGA GCCCCCTCCT GCCGTACCTC CTAAACCCTA CCTCAGAGAG 
GGCTGCATCC CTGAGGAGGA CCACCTGATC ACCCAGTCTG GTCCATCGCG CCCTCCCGTA 
CCTCGACCAA TGCCGCGTAA GATCTCCCAG CCCTTAATCG CAGCAACCAA GGACGAACAA 
GCTAAGGTGG CGTGGGAGCA CGGCATCAGT GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856649.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712103.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X2, mRNA.

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CloneID	OFu105380
Clone ID Related Accession (Same CDS sequence)	XM_012856649.1
Accession Version	XM_012856649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGTC CACCTGGTTT TGATAAGTCT 
GGCTCAGGAA TCAACAGAGG GATCCTGGCC TCTCACAACC CGATGAGTCC TGAGGGCTTC 
AAGCTCATGC ACCGACACAG TCCCATTGCT GTTTTGACTC CAGTGCGCAT TTCCTCCCCT 
TCTCTTTCCT CTAACTCCAG TGAAGCAGGA AATCTGCTGA TCCTGGCGGA TGGCATGGTG 
GTGGAGCACC CAGAGGACTT CTATCGTATG CAGGCGAGCC CCTCCTCTTC AAGTCTTAGC 
TCCACCCACT CTGCACCTCC TCAGATGATA AACTCCACCC CTCCCACATT AAGAGTGGGC 
TCCCCTTCTC TGCCAGACAA GTACAGACAC AATAGAGAAA TGCTGATGCT GCTGCCGCCC 
CACAGGGAGA GGCCGAGCAG TGCAATGTAC TCCAACATCA CAGAGAATGG ACAGCCACCA 
AACTTTCAGA GAGCCTTGTT TCATCAGGTG ATCGGTCCCT GTAAGCCCTG CAGTGACCCC 
AACCTCTCTG TGGCTGAGAA AGTCCTCACC ACTCCCAGCA GCTGGAGTTT GGACAGCGGC 
ACCAGGGAGG CTCTGCCTTT CCTTTCTTCT CATGTTGGCA GCGTTATGGC TCCACCGGTT 
CCCCCGCGGA ACGTGCCTCA TGGGCAGCAC CTGTCGATGC ACTTTGACGC TTTCCACCAC 
CAGGTCAGCG ACCTCCCTCC AGCTCTCCCT GCGCGCTCCT TAAGAAAGTC TCCCCTGCAC 
CCTATACCTG CCTCGCCCAC CAGTCCGCAG TCCGTCCTGG ATGGCAGTAA CTCCACACTC 
TCAGGCAGTG CCAGCTCTGG GGTTTCATCC CTCAGTGAGA GCAACTTTGG CGGCGCGACC 
TCCTCCTCAG ACCCCGCGGC CAGCCGCACA GACACCCTGG AGTCCGTCCC GAGCAGCCAG 
GCCTGGACCA CCGACCAGGA AGACTTGGAC TCTCCTTATC AGCCCGTCAG GTACAGCATG 
TCTGAGCCAG ACGTCCTGGA GGGAGTTAAA TCCCCGCCTT GCCGCAGCAA CTCTGCCCCA 
GGAGGAGTGA ACCCGCCCCA GGTCATCTAT CAGATCCAGT CTCAGGCTCC AGGTTTGGGC 
TTCACCGGAC CTCAGTCCCA ACAGCCGGAT GGACTGCAAC AGCAACAACA CCCGTTTTAC 
CACCACCACT TCCACCCACA CTACATTCCC CACAATCCAC CTCTCTATCA TCTCCATGAG 
CCCCCTCCTG CCGTACCTCC TAAACCCTAC CTCAGAGAGG GCTGCATCCC TGAGGAGGAC 
CACCTGATCA CCCAGTCTGG TCCATCGCGC CCTCCCGTAC CTCGACCAAT GCCGCGTAAG 
ATCTCCCAGC CCTTAATCGC AGCAACCAAG GACGAACAAG CTAAGGTGGC GTGGGAGCAC 
GGCATCAGTG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712103.1
CDS	35..6172
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856649.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856649.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGTC CACCTGGTTT TGATAAGTCT 
GGCTCAGGAA TCAACAGAGG GATCCTGGCC TCTCACAACC CGATGAGTCC TGAGGGCTTC 
AAGCTCATGC ACCGACACAG TCCCATTGCT GTTTTGACTC CAGTGCGCAT TTCCTCCCCT 
TCTCTTTCCT CTAACTCCAG TGAAGCAGGA AATCTGCTGA TCCTGGCGGA TGGCATGGTG 
GTGGAGCACC CAGAGGACTT CTATCGTATG CAGGCGAGCC CCTCCTCTTC AAGTCTTAGC 
TCCACCCACT CTGCACCTCC TCAGATGATA AACTCCACCC CTCCCACATT AAGAGTGGGC 
TCCCCTTCTC TGCCAGACAA GTACAGACAC AATAGAGAAA TGCTGATGCT GCTGCCGCCC 
CACAGGGAGA GGCCGAGCAG TGCAATGTAC TCCAACATCA CAGAGAATGG ACAGCCACCA 
AACTTTCAGA GAGCCTTGTT TCATCAGGTG ATCGGTCCCT GTAAGCCCTG CAGTGACCCC 
AACCTCTCTG TGGCTGAGAA AGTCCTCACC ACTCCCAGCA GCTGGAGTTT GGACAGCGGC 
ACCAGGGAGG CTCTGCCTTT CCTTTCTTCT CATGTTGGCA GCGTTATGGC TCCACCGGTT 
CCCCCGCGGA ACGTGCCTCA TGGGCAGCAC CTGTCGATGC ACTTTGACGC TTTCCACCAC 
CAGGTCAGCG ACCTCCCTCC AGCTCTCCCT GCGCGCTCCT TAAGAAAGTC TCCCCTGCAC 
CCTATACCTG CCTCGCCCAC CAGTCCGCAG TCCGTCCTGG ATGGCAGTAA CTCCACACTC 
TCAGGCAGTG CCAGCTCTGG GGTTTCATCC CTCAGTGAGA GCAACTTTGG CGGCGCGACC 
TCCTCCTCAG ACCCCGCGGC CAGCCGCACA GACACCCTGG AGTCCGTCCC GAGCAGCCAG 
GCCTGGACCA CCGACCAGGA AGACTTGGAC TCTCCTTATC AGCCCGTCAG GTACAGCATG 
TCTGAGCCAG ACGTCCTGGA GGGAGTTAAA TCCCCGCCTT GCCGCAGCAA CTCTGCCCCA 
GGAGGAGTGA ACCCGCCCCA GGTCATCTAT CAGATCCAGT CTCAGGCTCC AGGTTTGGGC 
TTCACCGGAC CTCAGTCCCA ACAGCCGGAT GGACTGCAAC AGCAACAACA CCCGTTTTAC 
CACCACCACT TCCACCCACA CTACATTCCC CACAATCCAC CTCTCTATCA TCTCCATGAG 
CCCCCTCCTG CCGTACCTCC TAAACCCTAC CTCAGAGAGG GCTGCATCCC TGAGGAGGAC 
CACCTGATCA CCCAGTCTGG TCCATCGCGC CCTCCCGTAC CTCGACCAAT GCCGCGTAAG 
ATCTCCCAGC CCTTAATCGC AGCAACCAAG GACGAACAAG CTAAGGTGGC GTGGGAGCAC 
GGCATCAGTG AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856650.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712104.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X3, mRNA.

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CloneID	OFu105381
Clone ID Related Accession (Same CDS sequence)	XM_012856650.1
Accession Version	XM_012856650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT TCTGTTGGAG AAGATTGAGC AGGAGACGTG GAAAGAAACA 
GGAATCTCGT TTGTTACATC GGTTACACGG TTGATGGAGC GACTGCTGGA CTACAGGGAC 
TGTATGAAGG GAGATGAGAC AGAAAACAAG AAGATTGGCT GCACAGTGAA CCTTCTTAAT 
TTCTACAAAT CGGAAATCAA CAAAGAGGAG ATGTACATCC GTTACATCCA CAAATTGTGT 
GACATGCATC TGCAGGCTGA GAACTACACT GAGGCTGCAT TCACGCTGCT GTTGTACTGG 
GAGCTGCTGC ACTGGGACGA GCGGCCAGTT AAAGAGTTCC TGCATTATCC TGCTCAGACT 
GAGTGGCACC GGAAGGAGGG CCTCTGCCGC AAAGTCATCC ACTATTTCAA CAAGGGAAAG 
TGTTGGGAAT TTGGCATTCC TCTGTGCAGA GAACTGGCCT TCCAGTATGA ATCTCTCTAC 
GACTACCAGA GTCTCAGCTG GATACGGAAA ATGGAGGCAG CATATTATGA CAATATCATG 
GAGCAGCAGC GTCTGGAGCC GGAGTTCTTC AGGGTGGGAT TCTACGGCAG GAAGTTTCCT 
TTCTTCCTCA GAAACAAAGA GTTTGTCTGC CGGGGCCATG ACTATGAAAG GTTAGAAGCC 
TTCCAACAAA GGATGCTGGG AGAATTCCCA CAAGCCATTG CAATGCAGCA CCCGAATCAG 
CCAGATGAGG CCATTCTGCA GTGTGATGCA CAGTACCTCC AGATTTACGC TGTGACACCG 
TTTCCAGAAA GCGTCACCGT CCTTCAGATG GACAGAGTTC CAGACCGCAT CAAGAGCTTC 
TATCGAGTGA ACAACGTTCG GCGATTTCGT TACGACCGGC CGTTCCACAA AGGACCCAAA 
GACAGAGAGA ATGAGTTTAA GAGTCTCTGG ATAGAAAGGA CGACTCTGAT CCTCACCCAT 
CCTCTGCCTG GAATTTCATG CTGGTTTGAG GTAGAAAAGA GGGAACTGGT GGAGGTGAGT 
CCATTGGAAA ACGCCATCTC TGTGGTGGAA AATAAGAACC AGGAGCTGCG GACTCTGATC 
AGTCAGTACC AACACAAGCA GCTGCACGGG AACATCAACC TGCTCAGCAT GACGCTGAAT 
GGAGTCATCG ACGCCGCCGT GAACGGGGGC ATCGCCAGAT ACCAGGAGGC TTTCTTTGAT 
AAGGAATACA TCACTAGTCA CCCTGAAGAC ACAGAGAAGA TCACGCAGCT CAAAGATCTC 
ATGCAGGAGC AGGTTCATAT CTTAGGGGTG GGCTTGGCTG TGCACGAGAA GTTGGTGCAC 
CCAGAAATGC GTCCCCTGCA CAAGAAGCTG ATCGATCAGT TCCAGATGAT GAGGAGCAGC 
CTCTGCCATG GCTTCTATGT TGCTTTTCAG GGTCCACCTG GTTTTGATAA GTCTGGCTCA 
GGAATCAACA GAGGGATCCT GGCCTCTCAC AACCCGATGA GTCCTGAGGG CTTCAAGCTC 
ATGCACCGAC ACAGTCCCAT TGCTGTTTTG ACTCCAGTGC GCATTTCCTC CCCTTCTCTT 
TCCTCTAACT CCAGTGAAGC AGGAAATCTG CTGATCCTGG CGGATGGCAT GGTGGTGGAG 
CACCCAGAGG ACTTCTATCG TATGCAGGCG AGCCCCTCCT CTTCAAGTCT TAGCTCCACC 
CACTCTGCAC CTCCTCAGAT GATAAACTCC ACCCCTCCCA CATTAAGAGT GGGCTCCCCT 
TCTCTGCCAG ACAAGTACAG ACACAATAGA GAAATGCTGA TGCTGCTGCC GCCCCACAGG 
GAGAGGCCGA GCAGTGCAAT GTACTCCAAC ATCACAGAGA ATGGACAGCC ACCAAACTTT 
CAGAGAGCCT TGTTTCATCA GGTGATCGGT CCCTGTAAGC CCTGCAGTGA CCCCAACCTC 
TCTGTGGCTG AGAAAGTCCT CACCACTCCC AGCAGCTGGA GTTTGGACAG CGGCACCAGG 
GAGGCTCTGC CTTTCCTTTC TTCTCATGTT GGCAGCGTTA TGGCTCCACC GGTTCCCCCG 
CGGAACGTGC CTCATGGGCA GCACCTGTCG ATGCACTTTG ACGCTTTCCA CCACCAGGTC 
AGCGACCTCC CTCCAGCTCT CCCTGCGCGC TCCTTAAGAA AGTCTCCCCT GCACCCTATA 
CCTGCCTCGC CCACCAGTCC GCAGTCCGTC CTGGATGGCA GTAACTCCAC ACTCTCAGGC 
AGTGCCAGCT CTGGGGTTTC ATCCCTCAGT GAGAGCAACT TTGGCGGCGC GACCTCCTCC 
TCAGACCCCG CGGCCAGCCG CACAGACACC CTGGAGTCCG TCCCGAGCAG CCAGGCCTGG 
ACCACCGACC AGGAAGACTT GGACTCTCCT TATCAGCCCG TCAGGTACAG CATGTCTGAG 
CCAGACGTCC TGGAGGGAGT TAAATCCCCG CCTTGCCGCA GCAACTCTGC CCCAGGAGGA 
GTGAACCCGC CCCAGGTCAT CTATCAGATC CAGTCTCAGG CTCCAGGTTT GGGCTTCACC 
GGACCTCAGT CCCAACAGCC GGATGGACTG CAACAGCAAC AACACCCGTT TTACCACCAC 
CACTTCCACC CACACTACAT TCCCCACAAT CCACCTCTCT ATCATCTCCA TGAGCCCCCT 
CCTGCCGTAC CTCCTAAACC CTACCTCAGA GAGGGCTGCA TCCCTGAGGA GGACCACCTG 
ATCACCCAGT CTGGTCCATC GCGCCCTCCC GTACCTCGAC CAATGCCGCG TAAGATCTCC 
CAGCCCTTAA TCGCAGCAAC CAAGGACGAA CAAGCTAAGG TGGCGTGGGA GCACGGCATC 
AGTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712104.1
CDS	35..6166
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856650.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856650.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT TCTGTTGGAG AAGATTGAGC AGGAGACGTG GAAAGAAACA 
GGAATCTCGT TTGTTACATC GGTTACACGG TTGATGGAGC GACTGCTGGA CTACAGGGAC 
TGTATGAAGG GAGATGAGAC AGAAAACAAG AAGATTGGCT GCACAGTGAA CCTTCTTAAT 
TTCTACAAAT CGGAAATCAA CAAAGAGGAG ATGTACATCC GTTACATCCA CAAATTGTGT 
GACATGCATC TGCAGGCTGA GAACTACACT GAGGCTGCAT TCACGCTGCT GTTGTACTGG 
GAGCTGCTGC ACTGGGACGA GCGGCCAGTT AAAGAGTTCC TGCATTATCC TGCTCAGACT 
GAGTGGCACC GGAAGGAGGG CCTCTGCCGC AAAGTCATCC ACTATTTCAA CAAGGGAAAG 
TGTTGGGAAT TTGGCATTCC TCTGTGCAGA GAACTGGCCT TCCAGTATGA ATCTCTCTAC 
GACTACCAGA GTCTCAGCTG GATACGGAAA ATGGAGGCAG CATATTATGA CAATATCATG 
GAGCAGCAGC GTCTGGAGCC GGAGTTCTTC AGGGTGGGAT TCTACGGCAG GAAGTTTCCT 
TTCTTCCTCA GAAACAAAGA GTTTGTCTGC CGGGGCCATG ACTATGAAAG GTTAGAAGCC 
TTCCAACAAA GGATGCTGGG AGAATTCCCA CAAGCCATTG CAATGCAGCA CCCGAATCAG 
CCAGATGAGG CCATTCTGCA GTGTGATGCA CAGTACCTCC AGATTTACGC TGTGACACCG 
TTTCCAGAAA GCGTCACCGT CCTTCAGATG GACAGAGTTC CAGACCGCAT CAAGAGCTTC 
TATCGAGTGA ACAACGTTCG GCGATTTCGT TACGACCGGC CGTTCCACAA AGGACCCAAA 
GACAGAGAGA ATGAGTTTAA GAGTCTCTGG ATAGAAAGGA CGACTCTGAT CCTCACCCAT 
CCTCTGCCTG GAATTTCATG CTGGTTTGAG GTAGAAAAGA GGGAACTGGT GGAGGTGAGT 
CCATTGGAAA ACGCCATCTC TGTGGTGGAA AATAAGAACC AGGAGCTGCG GACTCTGATC 
AGTCAGTACC AACACAAGCA GCTGCACGGG AACATCAACC TGCTCAGCAT GACGCTGAAT 
GGAGTCATCG ACGCCGCCGT GAACGGGGGC ATCGCCAGAT ACCAGGAGGC TTTCTTTGAT 
AAGGAATACA TCACTAGTCA CCCTGAAGAC ACAGAGAAGA TCACGCAGCT CAAAGATCTC 
ATGCAGGAGC AGGTTCATAT CTTAGGGGTG GGCTTGGCTG TGCACGAGAA GTTGGTGCAC 
CCAGAAATGC GTCCCCTGCA CAAGAAGCTG ATCGATCAGT TCCAGATGAT GAGGAGCAGC 
CTCTGCCATG GCTTCTATGT TGCTTTTCAG GGTCCACCTG GTTTTGATAA GTCTGGCTCA 
GGAATCAACA GAGGGATCCT GGCCTCTCAC AACCCGATGA GTCCTGAGGG CTTCAAGCTC 
ATGCACCGAC ACAGTCCCAT TGCTGTTTTG ACTCCAGTGC GCATTTCCTC CCCTTCTCTT 
TCCTCTAACT CCAGTGAAGC AGGAAATCTG CTGATCCTGG CGGATGGCAT GGTGGTGGAG 
CACCCAGAGG ACTTCTATCG TATGCAGGCG AGCCCCTCCT CTTCAAGTCT TAGCTCCACC 
CACTCTGCAC CTCCTCAGAT GATAAACTCC ACCCCTCCCA CATTAAGAGT GGGCTCCCCT 
TCTCTGCCAG ACAAGTACAG ACACAATAGA GAAATGCTGA TGCTGCTGCC GCCCCACAGG 
GAGAGGCCGA GCAGTGCAAT GTACTCCAAC ATCACAGAGA ATGGACAGCC ACCAAACTTT 
CAGAGAGCCT TGTTTCATCA GGTGATCGGT CCCTGTAAGC CCTGCAGTGA CCCCAACCTC 
TCTGTGGCTG AGAAAGTCCT CACCACTCCC AGCAGCTGGA GTTTGGACAG CGGCACCAGG 
GAGGCTCTGC CTTTCCTTTC TTCTCATGTT GGCAGCGTTA TGGCTCCACC GGTTCCCCCG 
CGGAACGTGC CTCATGGGCA GCACCTGTCG ATGCACTTTG ACGCTTTCCA CCACCAGGTC 
AGCGACCTCC CTCCAGCTCT CCCTGCGCGC TCCTTAAGAA AGTCTCCCCT GCACCCTATA 
CCTGCCTCGC CCACCAGTCC GCAGTCCGTC CTGGATGGCA GTAACTCCAC ACTCTCAGGC 
AGTGCCAGCT CTGGGGTTTC ATCCCTCAGT GAGAGCAACT TTGGCGGCGC GACCTCCTCC 
TCAGACCCCG CGGCCAGCCG CACAGACACC CTGGAGTCCG TCCCGAGCAG CCAGGCCTGG 
ACCACCGACC AGGAAGACTT GGACTCTCCT TATCAGCCCG TCAGGTACAG CATGTCTGAG 
CCAGACGTCC TGGAGGGAGT TAAATCCCCG CCTTGCCGCA GCAACTCTGC CCCAGGAGGA 
GTGAACCCGC CCCAGGTCAT CTATCAGATC CAGTCTCAGG CTCCAGGTTT GGGCTTCACC 
GGACCTCAGT CCCAACAGCC GGATGGACTG CAACAGCAAC AACACCCGTT TTACCACCAC 
CACTTCCACC CACACTACAT TCCCCACAAT CCACCTCTCT ATCATCTCCA TGAGCCCCCT 
CCTGCCGTAC CTCCTAAACC CTACCTCAGA GAGGGCTGCA TCCCTGAGGA GGACCACCTG 
ATCACCCAGT CTGGTCCATC GCGCCCTCCC GTACCTCGAC CAATGCCGCG TAAGATCTCC 
CAGCCCTTAA TCGCAGCAAC CAAGGACGAA CAAGCTAAGG TGGCGTGGGA GCACGGCATC 
AGTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856651.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712105.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X4, mRNA.

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CloneID	OFu105382
Clone ID Related Accession (Same CDS sequence)	XM_012856651.1
Accession Version	XM_012856651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGGAGAGT ACGTCTCGTG TCTCCTCTCG 
CTTCTTCGCC AGATGACTGA CATCCACTTT CAACACTTGA TGGAAAATTT TCAAAGCAAG 
GACGAGCTCA AGGAGTTCTT GTTGAAGATT TTGTGCGTGT TTAGAAATCT GATGAAGCTC 
AGTATTTTTC CCCGCGACTG GAACATCATG AGGCTGCTCA CCAGCAACAT CATCCTCACC 
ACAGCTCAGC AACTTGCTCC TGCGCTGCAC AAGAACTTTG CTGAAACAGA CTTTGACTTC 
AAGGTGTGGA ACTCCTACTT CAGTCTGGCC GTTCTCTACA TCAACCAGCC GAGTTTACAG 
CTGGAGAACC TGAGTCCTGT GAAGAGGAAG AAGATCTTAG ACAAGTATGG TGACATGAGA 
GTAATGATGA CTTATGAACT TTTCAACATG TGGCAGAACT TGGGAGAGAA TAAAATCCAT 
TTCATTCCTG GGATGATCGG ACCGTTCCTC GGAGTGACAC TGGTCCCTCA GGTGGAGGTG 
AGAAACATCA TGATTCCGAT CTTCCACGAC ATGATGGACT GGGAGCAGCG CAAAAATGGA 
AACTTCAAAC AGGTGGAGGC TGAACTAATT GACAAGTTGG ACAGTTTGGT GTCGGAGGGG 
AAGGGTGATG AGAACTACAG AGAGCTGTTT GGGCTGCTAA CCCAGCTGTT TGGGCCCTAT 
CCCAGTCTGT TGGAGAAGAT TGAGCAGGAG ACGTGGAAAG AAACAGGAAT CTCGTTTGTT 
ACATCGGTTA CACGGTTGAT GGAGCGACTG CTGGACTACA GGGACTGTAT GAAGGGAGAT 
GAGACAGAAA ACAAGAAGAT TGGCTGCACA GTGAACCTTC TTAATTTCTA CAAATCGGAA 
ATCAACAAAG AGGAGATGTA CATCCGTTAC ATCCACAAAT TGTGTGACAT GCATCTGCAG 
GCTGAGAACT ACACTGAGGC TGCATTCACG CTGCTGTTGT ACTGGGAGCT GCTGCACTGG 
GACGAGCGGC CAGTTAAAGA GTTCCTGCAT TATCCTGCTC AGACTGAGTG GCACCGGAAG 
GAGGGCCTCT GCCGCAAAGT CATCCACTAT TTCAACAAGG GAAAGTGTTG GGAATTTGGC 
ATTCCTCTGT GCAGAGAACT GGCCTTCCAG TATGAATCTC TCTACGACTA CCAGAGTCTC 
AGCTGGATAC GGAAAATGGA GGCAGCATAT TATGACAATA TCATGGAGCA GCAGCGTCTG 
GAGCCGGAGT TCTTCAGGGT GGGATTCTAC GGCAGGAAGT TTCCTTTCTT CCTCAGAAAC 
AAAGAGTTTG TCTGCCGGGG CCATGACTAT GAAAGGTTAG AAGCCTTCCA ACAAAGGATG 
CTGGGAGAAT TCCCACAAGC CATTGCAATG CAGCACCCGA ATCAGCCAGA TGAGGCCATT 
CTGCAGTGTG ATGCACAGTA CCTCCAGATT TACGCTGTGA CACCGTTTCC AGAAAGCGTC 
ACCGTCCTTC AGATGGACAG AGTTCCAGAC CGCATCAAGA GCTTCTATCG AGTGAACAAC 
GTTCGGCGAT TTCGTTACGA CCGGCCGTTC CACAAAGGAC CCAAAGACAG AGAGAATGAG 
TTTAAGAGTC TCTGGATAGA AAGGACGACT CTGATCCTCA CCCATCCTCT GCCTGGAATT 
TCATGCTGGT TTGAGGTAGA AAAGAGGGAA CTGGTGGAGG TGAGTCCATT GGAAAACGCC 
ATCTCTGTGG TGGAAAATAA GAACCAGGAG CTGCGGACTC TGATCAGTCA GTACCAACAC 
AAGCAGCTGC ACGGGAACAT CAACCTGCTC AGCATGACGC TGAATGGAGT CATCGACGCC 
GCCGTGAACG GGGGCATCGC CAGATACCAG GAGGCTTTCT TTGATAAGGA ATACATCACT 
AGTCACCCTG AAGACACAGA GAAGATCACG CAGCTCAAAG ATCTCATGCA GGAGCAGGTT 
CATATCTTAG GGGTGGGCTT GGCTGTGCAC GAGAAGTTGG TGCACCCAGA AATGCGTCCC 
CTGCACAAGA AGCTGATCGA TCAGTTCCAG ATGATGAGGA GCAGCCTCTG CCATGGCTTC 
TATGTTGCTT TTCAGGGTCC ACCTGGTTTT GATAAGTCTG GCTCAGGAAT CAACAGAGGG 
ATCCTGGCCT CTCACAACCC GATGAGTCCT GAGGGCTTCA AGCTCATGCA CCGACACAGT 
CCCATTGCTG TTTTGACTCC AGTGCGCATT TCCTCCCCTT CTCTTTCCTC TAACTCCAGT 
GAAGCAGGAA ATCTGCTGAT CCTGGCGGAT GGCATGGTGG TGGAGCACCC AGAGGACTTC 
TATCGTATGC AGGCGAGCCC CTCCTCTTCA AGTCTTAGCT CCACCCACTC TGCACCTCCT 
CAGATGATAA ACTCCACCCC TCCCACATTA AGAGTGGGCT CCCCTTCTCT GCCAGACAAG 
TACAGACACA ATAGAGAAAT GCTGATGCTG CTGCCGCCCC ACAGGGAGAG GCCGAGCAGT 
GCAATGTACT CCAACATCAC AGAGAATGGA CAGCCACCAA ACTTTCAGAG AGCCTTGTTT 
CATCAGGTGA TCGGTCCCTG TAAGCCCTGC AGTGACCCCA ACCTCTCTGT GGCTGAGAAA 
GTCCTCACCA CTCCCAGCAG CTGGAGTTTG GACAGCGGCA CCAGGGAGGC TCTGCCTTTC 
CTTTCTTCTC ATGTTGGCAG CGTTATGGCT CCACCGGTTC CCCCGCGGAA CGTGCCTCAT 
GGGCAGCACC TGTCGATGCA CTTTGACGCT TTCCACCACC AGGTCAGCGA CCTCCCTCCA 
GCTCTCCCTG CGCGCTCCTT AAGAAAGTCT CCCCTGCACC CTATACCTGC CTCGCCCACC 
AGTCCGCAGT CCGTCCTGGA TGGCAGTAAC TCCACACTCT CAGGCAGTGC CAGCTCTGGG 
GTTTCATCCC TCAGTGAGAG CAACTTTGGC GGCGCGACCT CCTCCTCAGA CCCCGCGGCC 
AGCCGCACAG ACACCCTGGA GTCCGTCCCG AGCAGCCAGG CCTGGACCAC CGACCAGGAA 
GACTTGGACT CTCCTTATCA GCCCGTCAGG TACAGCATGT CTGAGCCAGA CGTCCTGGAG 
GGAGTTAAAT CCCCGCCTTG CCGCAGCAAC TCTGCCCCAG GAGGAGTGAA CCCGCCCCAG 
GTCATCTATC AGATCCAGTC TCAGGCTCCA GGTTTGGGCT TCACCGGACC TCAGTCCCAA 
CAGCCGGATG GACTGCAACA GCAACAACAC CCGTTTTACC ACCACCACTT CCACCCACAC 
TACATTCCCC ACAATCCACC TCTCTATCAT CTCCATGAGC CCCCTCCTGC CGTACCTCCT 
AAACCCTACC TCAGAGAGGG CTGCATCCCT GAGGAGGACC ACCTGATCAC CCAGTCTGGT 
CCATCGCGCC CTCCCGTACC TCGACCAATG CCGCGTAAGA TCTCCCAGCC CTTAATCGCA 
GCAACCAAGG ACGAACAAGC TAAGGTGGCG TGGGAGCACG GCATCAGTGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712105.1
CDS	35..6151
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSPIAVLTPVRISSPSLSSNSSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856651.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856651.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGGAGAGT ACGTCTCGTG TCTCCTCTCG 
CTTCTTCGCC AGATGACTGA CATCCACTTT CAACACTTGA TGGAAAATTT TCAAAGCAAG 
GACGAGCTCA AGGAGTTCTT GTTGAAGATT TTGTGCGTGT TTAGAAATCT GATGAAGCTC 
AGTATTTTTC CCCGCGACTG GAACATCATG AGGCTGCTCA CCAGCAACAT CATCCTCACC 
ACAGCTCAGC AACTTGCTCC TGCGCTGCAC AAGAACTTTG CTGAAACAGA CTTTGACTTC 
AAGGTGTGGA ACTCCTACTT CAGTCTGGCC GTTCTCTACA TCAACCAGCC GAGTTTACAG 
CTGGAGAACC TGAGTCCTGT GAAGAGGAAG AAGATCTTAG ACAAGTATGG TGACATGAGA 
GTAATGATGA CTTATGAACT TTTCAACATG TGGCAGAACT TGGGAGAGAA TAAAATCCAT 
TTCATTCCTG GGATGATCGG ACCGTTCCTC GGAGTGACAC TGGTCCCTCA GGTGGAGGTG 
AGAAACATCA TGATTCCGAT CTTCCACGAC ATGATGGACT GGGAGCAGCG CAAAAATGGA 
AACTTCAAAC AGGTGGAGGC TGAACTAATT GACAAGTTGG ACAGTTTGGT GTCGGAGGGG 
AAGGGTGATG AGAACTACAG AGAGCTGTTT GGGCTGCTAA CCCAGCTGTT TGGGCCCTAT 
CCCAGTCTGT TGGAGAAGAT TGAGCAGGAG ACGTGGAAAG AAACAGGAAT CTCGTTTGTT 
ACATCGGTTA CACGGTTGAT GGAGCGACTG CTGGACTACA GGGACTGTAT GAAGGGAGAT 
GAGACAGAAA ACAAGAAGAT TGGCTGCACA GTGAACCTTC TTAATTTCTA CAAATCGGAA 
ATCAACAAAG AGGAGATGTA CATCCGTTAC ATCCACAAAT TGTGTGACAT GCATCTGCAG 
GCTGAGAACT ACACTGAGGC TGCATTCACG CTGCTGTTGT ACTGGGAGCT GCTGCACTGG 
GACGAGCGGC CAGTTAAAGA GTTCCTGCAT TATCCTGCTC AGACTGAGTG GCACCGGAAG 
GAGGGCCTCT GCCGCAAAGT CATCCACTAT TTCAACAAGG GAAAGTGTTG GGAATTTGGC 
ATTCCTCTGT GCAGAGAACT GGCCTTCCAG TATGAATCTC TCTACGACTA CCAGAGTCTC 
AGCTGGATAC GGAAAATGGA GGCAGCATAT TATGACAATA TCATGGAGCA GCAGCGTCTG 
GAGCCGGAGT TCTTCAGGGT GGGATTCTAC GGCAGGAAGT TTCCTTTCTT CCTCAGAAAC 
AAAGAGTTTG TCTGCCGGGG CCATGACTAT GAAAGGTTAG AAGCCTTCCA ACAAAGGATG 
CTGGGAGAAT TCCCACAAGC CATTGCAATG CAGCACCCGA ATCAGCCAGA TGAGGCCATT 
CTGCAGTGTG ATGCACAGTA CCTCCAGATT TACGCTGTGA CACCGTTTCC AGAAAGCGTC 
ACCGTCCTTC AGATGGACAG AGTTCCAGAC CGCATCAAGA GCTTCTATCG AGTGAACAAC 
GTTCGGCGAT TTCGTTACGA CCGGCCGTTC CACAAAGGAC CCAAAGACAG AGAGAATGAG 
TTTAAGAGTC TCTGGATAGA AAGGACGACT CTGATCCTCA CCCATCCTCT GCCTGGAATT 
TCATGCTGGT TTGAGGTAGA AAAGAGGGAA CTGGTGGAGG TGAGTCCATT GGAAAACGCC 
ATCTCTGTGG TGGAAAATAA GAACCAGGAG CTGCGGACTC TGATCAGTCA GTACCAACAC 
AAGCAGCTGC ACGGGAACAT CAACCTGCTC AGCATGACGC TGAATGGAGT CATCGACGCC 
GCCGTGAACG GGGGCATCGC CAGATACCAG GAGGCTTTCT TTGATAAGGA ATACATCACT 
AGTCACCCTG AAGACACAGA GAAGATCACG CAGCTCAAAG ATCTCATGCA GGAGCAGGTT 
CATATCTTAG GGGTGGGCTT GGCTGTGCAC GAGAAGTTGG TGCACCCAGA AATGCGTCCC 
CTGCACAAGA AGCTGATCGA TCAGTTCCAG ATGATGAGGA GCAGCCTCTG CCATGGCTTC 
TATGTTGCTT TTCAGGGTCC ACCTGGTTTT GATAAGTCTG GCTCAGGAAT CAACAGAGGG 
ATCCTGGCCT CTCACAACCC GATGAGTCCT GAGGGCTTCA AGCTCATGCA CCGACACAGT 
CCCATTGCTG TTTTGACTCC AGTGCGCATT TCCTCCCCTT CTCTTTCCTC TAACTCCAGT 
GAAGCAGGAA ATCTGCTGAT CCTGGCGGAT GGCATGGTGG TGGAGCACCC AGAGGACTTC 
TATCGTATGC AGGCGAGCCC CTCCTCTTCA AGTCTTAGCT CCACCCACTC TGCACCTCCT 
CAGATGATAA ACTCCACCCC TCCCACATTA AGAGTGGGCT CCCCTTCTCT GCCAGACAAG 
TACAGACACA ATAGAGAAAT GCTGATGCTG CTGCCGCCCC ACAGGGAGAG GCCGAGCAGT 
GCAATGTACT CCAACATCAC AGAGAATGGA CAGCCACCAA ACTTTCAGAG AGCCTTGTTT 
CATCAGGTGA TCGGTCCCTG TAAGCCCTGC AGTGACCCCA ACCTCTCTGT GGCTGAGAAA 
GTCCTCACCA CTCCCAGCAG CTGGAGTTTG GACAGCGGCA CCAGGGAGGC TCTGCCTTTC 
CTTTCTTCTC ATGTTGGCAG CGTTATGGCT CCACCGGTTC CCCCGCGGAA CGTGCCTCAT 
GGGCAGCACC TGTCGATGCA CTTTGACGCT TTCCACCACC AGGTCAGCGA CCTCCCTCCA 
GCTCTCCCTG CGCGCTCCTT AAGAAAGTCT CCCCTGCACC CTATACCTGC CTCGCCCACC 
AGTCCGCAGT CCGTCCTGGA TGGCAGTAAC TCCACACTCT CAGGCAGTGC CAGCTCTGGG 
GTTTCATCCC TCAGTGAGAG CAACTTTGGC GGCGCGACCT CCTCCTCAGA CCCCGCGGCC 
AGCCGCACAG ACACCCTGGA GTCCGTCCCG AGCAGCCAGG CCTGGACCAC CGACCAGGAA 
GACTTGGACT CTCCTTATCA GCCCGTCAGG TACAGCATGT CTGAGCCAGA CGTCCTGGAG 
GGAGTTAAAT CCCCGCCTTG CCGCAGCAAC TCTGCCCCAG GAGGAGTGAA CCCGCCCCAG 
GTCATCTATC AGATCCAGTC TCAGGCTCCA GGTTTGGGCT TCACCGGACC TCAGTCCCAA 
CAGCCGGATG GACTGCAACA GCAACAACAC CCGTTTTACC ACCACCACTT CCACCCACAC 
TACATTCCCC ACAATCCACC TCTCTATCAT CTCCATGAGC CCCCTCCTGC CGTACCTCCT 
AAACCCTACC TCAGAGAGGG CTGCATCCCT GAGGAGGACC ACCTGATCAC CCAGTCTGGT 
CCATCGCGCC CTCCCGTACC TCGACCAATG CCGCGTAAGA TCTCCCAGCC CTTAATCGCA 
GCAACCAAGG ACGAACAAGC TAAGGTGGCG TGGGAGCACG GCATCAGTGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856652.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712106.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X5, mRNA.

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CloneID	OFu105383
Clone ID Related Accession (Same CDS sequence)	XM_012856652.1
Accession Version	XM_012856652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	dedicator of cytokinesis protein 3 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGCT TCTATGTTGC TTTTCAGGGT 
CCACCTGGTT TTGATAAGTC TGGCTCAGGA ATCAACAGAG GGATCCTGGC CTCTCACAAC 
CCGATGAGTC CTGAGGGCTT CAAGCTCATG CACCGACACA GTGAAGCAGG AAATCTGCTG 
ATCCTGGCGG ATGGCATGGT GGTGGAGCAC CCAGAGGACT TCTATCGTAT GCAGGCGAGC 
CCCTCCTCTT CAAGTCTTAG CTCCACCCAC TCTGCACCTC CTCAGATGAT AAACTCCACC 
CCTCCCACAT TAAGAGTGGG CTCCCCTTCT CTGCCAGACA AGTACAGACA CAATAGAGAA 
ATGCTGATGC TGCTGCCGCC CCACAGGGAG AGGCCGAGCA GTGCAATGTA CTCCAACATC 
ACAGAGAATG GACAGCCACC AAACTTTCAG AGAGCCTTGT TTCATCAGGT GATCGGTCCC 
TGTAAGCCCT GCAGTGACCC CAACCTCTCT GTGGCTGAGA AAGTCCTCAC CACTCCCAGC 
AGCTGGAGTT TGGACAGCGG CACCAGGGAG GCTCTGCCTT TCCTTTCTTC TCATGTTGGC 
AGCGTTATGG CTCCACCGGT TCCCCCGCGG AACGTGCCTC ATGGGCAGCA CCTGTCGATG 
CACTTTGACG CTTTCCACCA CCAGGTCAGC GACCTCCCTC CAGCTCTCCC TGCGCGCTCC 
TTAAGAAAGT CTCCCCTGCA CCCTATACCT GCCTCGCCCA CCAGTCCGCA GTCCGTCCTG 
GATGGCAGTA ACTCCACACT CTCAGGCAGT GCCAGCTCTG GGGTTTCATC CCTCAGTGAG 
AGCAACTTTG GCGGCGCGAC CTCCTCCTCA GACCCCGCGG CCAGCCGCAC AGACACCCTG 
GAGTCCGTCC CGAGCAGCCA GGCCTGGACC ACCGACCAGG AAGACTTGGA CTCTCCTTAT 
CAGCCCGTCA GGTACAGCAT GTCTGAGCCA GACGTCCTGG AGGGAGTTAA ATCCCCGCCT 
TGCCGCAGCA ACTCTGCCCC AGGAGGAGTG AACCCGCCCC AGGTCATCTA TCAGATCCAG 
TCTCAGGCTC CAGGTTTGGG CTTCACCGGA CCTCAGTCCC AACAGCCGGA TGGACTGCAA 
CAGCAACAAC ACCCGTTTTA CCACCACCAC TTCCACCCAC ACTACATTCC CCACAATCCA 
CCTCTCTATC ATCTCCATGA GCCCCCTCCT GCCGTACCTC CTAAACCCTA CCTCAGAGAG 
GGCTGCATCC CTGAGGAGGA CCACCTGATC ACCCAGTCTG GTCCATCGCG CCCTCCCGTA 
CCTCGACCAA TGCCGCGTAA GATCTCCCAG CCCTTAATCG CAGCAACCAA GGACGAACAA 
GCTAAGGTGG CGTGGGAGCA CGGCATCAGT GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712106.1
CDS	35..6133
Translation	MMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856652.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856652.1

ORF Insert Sequence:

ATGATGGAGC TGATCGACCT GAGGAGGCAG CTGCTGTCTG GTCACCTCAC CCAGGACCAG 
AGCAGAGACG TCAAGAGACA CATCACTGTC AGACTCGACT GGGGCAATGA ACACCTGGGT 
CTGGACCTGG TGCCAAGAAA GGAGTTTGAG ATGGTTGATG AAGACCAGAT AAGTGTGTCA 
GACTTGTATA AAATGCATTT GTCCAGCAGA CACAGCGTCC AGCAGAGCAG CACACAGGGT 
GAGAATAGTC GGCAGAGACA TGGAGAACCT TGCAGGGTTC CTGTACCCCA CCACCTACTG 
GTCAACCTGA AGAGCTTCAC CTACAACAGC ATAGGCGAGG ACACAGACAT ATTTTTCTCT 
CTGTTTGATC TGAGGGAGGG AAAGACCATC AGTGAGAAAT TAATGGTGAG ACTAAACAAA 
AACGGAGGAC CAAAGAATCC GGAGAAGGTG GATCGGCTGT GTGCTCTGTT CACGGACCTG 
AGCAACAAAG ATATGAAGAG AGATCTCTAC ATCGTATCAC AAGTTATTCG AACAGGTCGC 
ATGCTGCTGA ATGACAGTAA GAAAGGTCCG GCACATGTTC AGTACCGCAG ACCATACGGC 
TGCGCTGTTC TGGCCATGAG TGACGTGCTG CAGATCATCT CAGAGCTCAA AGAGGAGAAA 
GACTTTGTTC TTAAAGTCTA CACGTGTAAC AATGAGAATG AATGGTACCA GATTCATGAA 
AACATCATCC GCAAGTCGAG CACCAAATAC ACAGCTCCCA GCACCAACTA CGGACTGATC 
ATCTCCCTGC AGCTGCTCAG AGGCGACATG GAGCAGGTCC GCAGAGAGAA CCCTCTCATT 
TTCAGCAGGG GGGTGGCCAT CACTCGGAAA CTGGGATTCC CAGATGTCAT CATGCCAGGC 
GACATCCGTA ATGACCTCTA CCTTACTCTG GAGCGAGGAG ACTTTGAGAG AGGAGGGAAG 
AGTGTTCAGA AAAACATCGA AGTGACTATG TATGTCATCT ACGCAGATGG TGAAATACTG 
AAGGACTGCA TCAGTCTGGG TTCAGGCGAG CCCAACATCC CTGAGTATCG CTCCTTCGTC 
CTCTACCACA ACAACAGCCC CCGCTGGAGT GAAGTGATTA AGCTGCCGAT TCCCATCGAT 
CGTTTCAGAG GCTCCCACCT CCGCTTTGAG TTCAGACATT GCTCCACAAA GGATAAAGGA 
GAAAAAAAAC TTTATGGCTT TGCCTTCACT CCTTTAATGA GGGAGGATGG AACAACTTTA 
TCTGACGAGA GTCATGAGCT CTATGTCTAC AAGTGCGATG AGAACACAAC CTTCAGTAAC 
CACGCCCTCT ACCTGGGCCT TCCCTGCTGT AAGGACGACT TCAACAGCTG TCCCAACATC 
CCCTCCAGCC TCATCTTCCA GCGAAGCACC AAGGAGACGT TCTGGATCTC CACGCAGCTC 
TCCTCCACCA AGCTCACTCA GAACGTGGAC CTCCTGGCCC TGCTGAAGTG GAAGGCCCAT 
CCTGAACGGG TCATGGACAT CCTGGGGCGT CTGCGACACG TCAGTGGTGA AGAGATAGTC 
AAGTTTCTCC AGGATATTCT GGACACATTA TTCTCCATCT TGGATGACAA CACAGACAAA 
TATGGACCCC TTGTGTTTCA GTCATTGGTT TTTATCATAA ACTTGCTCAG GGACAGTAAA 
TACTACCACT TCAGGCCGGT GATGGACACA TATATCCAGA AACACTTTGC CGGAGCTCTG 
GCCTACAAAG AGCTGATTCG CTGTCTGAAG TGGTACATGG ATCGTTCTGC CGAGGTGGTC 
AGACAGGACC ACATTCAGGA AGCAATGAGG GCTCTGGAGT ATCTGTTCAA GTTTATCATC 
CAGTCTCGGA TCCTCTACTC CCGGGCCACG TGCGGGATGG AGGAGGAGCA GTTTCGGACC 
AGCATCCAGG AACTCTTTCA GTCCATTCGC TTCGTCCTCA GCCTGGACAG CCGCAACTCG 
GAGACCCTCA TATTCACTCA GGGCTCCCAG CCTTGTCCCG GCCCCAATCA GGCTCCAGGC 
CCCTGCATAG GGCCTGCAGG CCCGTTAGCA CTAGTCTCTG CCCTGGGGCA AATCCAGACC 
CCTGTCCCAG GACAGGTTCC TGGGAGCGGT CCATCTAACT GCTCTCAGGC CTGGAGCTCC 
AACGCTCTGC GGCCGGCTTC GCTGTCAGCT GCACTGTTAA ACTCATTCCC TGCCATCTTC 
GACGAGTTGC TGCAGATGTT CACGGTGCAG GAGGTGGCAG AGTTTGTGCG GGGCACTTTG 
GGCAGCATGC CGTCCACGGT GCACATAGGA CAATCGATGG ACGTGGTGAA GCTGCAGTCC 
ATAGCCCGAA CCGTGGACAG TAGGCTCTTC TCTTTCCCTG AGTCTCGAAG GATCCTGCTT 
CCCGTGGTCC TGCATCACAT CCATCTTCAT CTGAGACAAC AGAAAGAGCT GCTCATCTGC 
TCAGGGATAC TCAGCTCCAT TTTCTCCATT ATCAAGAGCA GCTCACTGGA CACGTCGGTG 
CAGGAAGAGG TGGAGATGAT GGTTGAGTCT CTGCTGGACG TCCTCCTGCA GACGCTTCTG 
TCGATCATGA GCAAGAGCCA ATCGCAGGAG GCGGTAAGGG GTCAACGCTG TCCGCAGTGC 
ACCGCGGAGA TCACTGGAGA GTACGTCTCG TGTCTCCTCT CGCTTCTTCG CCAGATGACT 
GACATCCACT TTCAACACTT GATGGAAAAT TTTCAAAGCA AGGACGAGCT CAAGGAGTTC 
TTGTTGAAGA TTTTGTGCGT GTTTAGAAAT CTGATGAAGC TCAGTATTTT TCCCCGCGAC 
TGGAACATCA TGAGGCTGCT CACCAGCAAC ATCATCCTCA CCACAGCTCA GCAACTTGCT 
CCTGCGCTGC ACAAGAACTT TGCTGAAACA GACTTTGACT TCAAGGTGTG GAACTCCTAC 
TTCAGTCTGG CCGTTCTCTA CATCAACCAG CCGAGTTTAC AGCTGGAGAA CCTGAGTCCT 
GTGAAGAGGA AGAAGATCTT AGACAAGTAT GGTGACATGA GAGTAATGAT GACTTATGAA 
CTTTTCAACA TGTGGCAGAA CTTGGGAGAG AATAAAATCC ATTTCATTCC TGGGATGATC 
GGACCGTTCC TCGGAGTGAC ACTGGTCCCT CAGGTGGAGG TGAGAAACAT CATGATTCCG 
ATCTTCCACG ACATGATGGA CTGGGAGCAG CGCAAAAATG GAAACTTCAA ACAGGTGGAG 
GCTGAACTAA TTGACAAGTT GGACAGTTTG GTGTCGGAGG GGAAGGGTGA TGAGAACTAC 
AGAGAGCTGT TTGGGCTGCT AACCCAGCTG TTTGGGCCCT ATCCCAGTCT GTTGGAGAAG 
ATTGAGCAGG AGACGTGGAA AGAAACAGGA ATCTCGTTTG TTACATCGGT TACACGGTTG 
ATGGAGCGAC TGCTGGACTA CAGGGACTGT ATGAAGGGAG ATGAGACAGA AAACAAGAAG 
ATTGGCTGCA CAGTGAACCT TCTTAATTTC TACAAATCGG AAATCAACAA AGAGGAGATG 
TACATCCGTT ACATCCACAA ATTGTGTGAC ATGCATCTGC AGGCTGAGAA CTACACTGAG 
GCTGCATTCA CGCTGCTGTT GTACTGGGAG CTGCTGCACT GGGACGAGCG GCCAGTTAAA 
GAGTTCCTGC ATTATCCTGC TCAGACTGAG TGGCACCGGA AGGAGGGCCT CTGCCGCAAA 
GTCATCCACT ATTTCAACAA GGGAAAGTGT TGGGAATTTG GCATTCCTCT GTGCAGAGAA 
CTGGCCTTCC AGTATGAATC TCTCTACGAC TACCAGAGTC TCAGCTGGAT ACGGAAAATG 
GAGGCAGCAT ATTATGACAA TATCATGGAG CAGCAGCGTC TGGAGCCGGA GTTCTTCAGG 
GTGGGATTCT ACGGCAGGAA GTTTCCTTTC TTCCTCAGAA ACAAAGAGTT TGTCTGCCGG 
GGCCATGACT ATGAAAGGTT AGAAGCCTTC CAACAAAGGA TGCTGGGAGA ATTCCCACAA 
GCCATTGCAA TGCAGCACCC GAATCAGCCA GATGAGGCCA TTCTGCAGTG TGATGCACAG 
TACCTCCAGA TTTACGCTGT GACACCGTTT CCAGAAAGCG TCACCGTCCT TCAGATGGAC 
AGAGTTCCAG ACCGCATCAA GAGCTTCTAT CGAGTGAACA ACGTTCGGCG ATTTCGTTAC 
GACCGGCCGT TCCACAAAGG ACCCAAAGAC AGAGAGAATG AGTTTAAGAG TCTCTGGATA 
GAAAGGACGA CTCTGATCCT CACCCATCCT CTGCCTGGAA TTTCATGCTG GTTTGAGGTA 
GAAAAGAGGG AACTGGTGGA GGTGAGTCCA TTGGAAAACG CCATCTCTGT GGTGGAAAAT 
AAGAACCAGG AGCTGCGGAC TCTGATCAGT CAGTACCAAC ACAAGCAGCT GCACGGGAAC 
ATCAACCTGC TCAGCATGAC GCTGAATGGA GTCATCGACG CCGCCGTGAA CGGGGGCATC 
GCCAGATACC AGGAGGCTTT CTTTGATAAG GAATACATCA CTAGTCACCC TGAAGACACA 
GAGAAGATCA CGCAGCTCAA AGATCTCATG CAGGAGCAGG TTCATATCTT AGGGGTGGGC 
TTGGCTGTGC ACGAGAAGTT GGTGCACCCA GAAATGCGTC CCCTGCACAA GAAGCTGATC 
GATCAGTTCC AGATGATGAG GAGCAGCCTC TGCCATGGCT TCTATGTTGC TTTTCAGGGT 
CCACCTGGTT TTGATAAGTC TGGCTCAGGA ATCAACAGAG GGATCCTGGC CTCTCACAAC 
CCGATGAGTC CTGAGGGCTT CAAGCTCATG CACCGACACA GTGAAGCAGG AAATCTGCTG 
ATCCTGGCGG ATGGCATGGT GGTGGAGCAC CCAGAGGACT TCTATCGTAT GCAGGCGAGC 
CCCTCCTCTT CAAGTCTTAG CTCCACCCAC TCTGCACCTC CTCAGATGAT AAACTCCACC 
CCTCCCACAT TAAGAGTGGG CTCCCCTTCT CTGCCAGACA AGTACAGACA CAATAGAGAA 
ATGCTGATGC TGCTGCCGCC CCACAGGGAG AGGCCGAGCA GTGCAATGTA CTCCAACATC 
ACAGAGAATG GACAGCCACC AAACTTTCAG AGAGCCTTGT TTCATCAGGT GATCGGTCCC 
TGTAAGCCCT GCAGTGACCC CAACCTCTCT GTGGCTGAGA AAGTCCTCAC CACTCCCAGC 
AGCTGGAGTT TGGACAGCGG CACCAGGGAG GCTCTGCCTT TCCTTTCTTC TCATGTTGGC 
AGCGTTATGG CTCCACCGGT TCCCCCGCGG AACGTGCCTC ATGGGCAGCA CCTGTCGATG 
CACTTTGACG CTTTCCACCA CCAGGTCAGC GACCTCCCTC CAGCTCTCCC TGCGCGCTCC 
TTAAGAAAGT CTCCCCTGCA CCCTATACCT GCCTCGCCCA CCAGTCCGCA GTCCGTCCTG 
GATGGCAGTA ACTCCACACT CTCAGGCAGT GCCAGCTCTG GGGTTTCATC CCTCAGTGAG 
AGCAACTTTG GCGGCGCGAC CTCCTCCTCA GACCCCGCGG CCAGCCGCAC AGACACCCTG 
GAGTCCGTCC CGAGCAGCCA GGCCTGGACC ACCGACCAGG AAGACTTGGA CTCTCCTTAT 
CAGCCCGTCA GGTACAGCAT GTCTGAGCCA GACGTCCTGG AGGGAGTTAA ATCCCCGCCT 
TGCCGCAGCA ACTCTGCCCC AGGAGGAGTG AACCCGCCCC AGGTCATCTA TCAGATCCAG 
TCTCAGGCTC CAGGTTTGGG CTTCACCGGA CCTCAGTCCC AACAGCCGGA TGGACTGCAA 
CAGCAACAAC ACCCGTTTTA CCACCACCAC TTCCACCCAC ACTACATTCC CCACAATCCA 
CCTCTCTATC ATCTCCATGA GCCCCCTCCT GCCGTACCTC CTAAACCCTA CCTCAGAGAG 
GGCTGCATCC CTGAGGAGGA CCACCTGATC ACCCAGTCTG GTCCATCGCG CCCTCCCGTA 
CCTCGACCAA TGCCGCGTAA GATCTCCCAG CCCTTAATCG CAGCAACCAA GGACGAACAA 
GCTAAGGTGG CGTGGGAGCA CGGCATCAGT GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_012856652.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712106.2 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X1, mRNA.

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CloneID	OFu130679
Clone ID Related Accession (Same CDS sequence)	XM_012856652.2
Accession Version	XM_012856652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012856652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGCTTCTATG TTGCTTTTCA GGGTCCACCT 
GGTTTTGATA AGTCTGGCTC AGGAATCAAC AGAGGGATCC TGGCCTCTCA CAACCCGATG 
AGTCCTGAGG GCTTCAAGCT CATGCACCGA CACAGTGAAG CAGGAAATCT GCTGATCCTG 
GCGGATGGCA TGGTGGTGGA GCACCCAGAG GACTTCTATC GTATGCAGGC GAGCCCCTCC 
TCTTCAAGTC TTAGCTCCAC CCACTCTGCA CCTCCTCAGA TGATAAACTC CACCCCTCCC 
ACATTAAGAG TGGGCTCCCC TTCTCTGCCA GACAAGTACA GACACAATAG AGAAATGCTG 
ATGCTGCTGC CGCCCCACAG GGAGAGGCCG AGCAGTGCAA TGTACTCCAA CATCACAGAG 
AATGGACAGC CACCAAACTT TCAGAGAGCC TTGTTTCATC AGGTGATCGG TCCCTGTAAG 
CCCTGCAGTG ACCCCAACCT CTCTGTGGCT GAGAAAGTCC TCACCACTCC CAGCAGCTGG 
AGTTTGGACA GCGGCACCAG GGAGGCTCTG CCTTTCCTTT CTTCTCATGT TGGCAGCGTT 
ATGGCTCCAC CGGTTCCCCC GCGGAACGTG CCTCATGGGC AGCACCTGTC GATGCACTTT 
GACGCTTTCC ACCACCAGGT CAGCGACCTC CCTCCAGCTC TCCCTGCGCG CTCCTTAAGA 
AAGTCTCCCC TGCACCCTAT ACCTGCCTCG CCCACCAGTC CGCAGTCCGT CCTGGATGGC 
AGTAACTCCA CACTCTCAGG CAGTGCCAGC TCTGGGGTTT CATCCCTCAG TGAGAGCAAC 
TTTGGCGGCG CGACCTCCTC CTCAGACCCC GCGGCCAGCC GCACAGACAC CCTGGAGTCC 
GTCCCGAGCA GCCAGGCCTG GACCACCGAC CAGGAAGACT TGGACTCTCC TTATCAGCCC 
GTCAGGTACA GCATGTCTGA GCCAGACGTC CTGGAGGGAG TTAAATCCCC GCCTTGCCGC 
AGCAACTCTG CCCCAGGAGG AGTGAACCCG CCCCAGGTCA TCTATCAGAT CCAGTCTCAG 
GCTCCAGGTT TGGGCTTCAC CGGACCTCAG TCCCAACAGC CGGATGGACT GCAACAGCAA 
CAACACCCGT TTTACCACCA CCACTTCCAC CCACACTACA TTCCCCACAA TCCACCTCTC 
TATCATCTCC ATGAGCCCCC TCCTGCCGTA CCTCCTAAAC CCTACCTCAG AGAGGGCTGC 
ATCCCTGAGG AGGACCACCT GATCACCCAG TCTGGTCCAT CGCGCCCTCC CGTACCTCGA 
CCAATGCCGC GTAAGATCTC CCAGCCCTTA ATCGCAGCAA CCAAGGACGA ACAAGCTAAG 
GTGGCGTGGG AGCACGGCAT CAGTGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712106.2
CDS	185..6637
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_012856652.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856652.2

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGCTTCTATG TTGCTTTTCA GGGTCCACCT 
GGTTTTGATA AGTCTGGCTC AGGAATCAAC AGAGGGATCC TGGCCTCTCA CAACCCGATG 
AGTCCTGAGG GCTTCAAGCT CATGCACCGA CACAGTGAAG CAGGAAATCT GCTGATCCTG 
GCGGATGGCA TGGTGGTGGA GCACCCAGAG GACTTCTATC GTATGCAGGC GAGCCCCTCC 
TCTTCAAGTC TTAGCTCCAC CCACTCTGCA CCTCCTCAGA TGATAAACTC CACCCCTCCC 
ACATTAAGAG TGGGCTCCCC TTCTCTGCCA GACAAGTACA GACACAATAG AGAAATGCTG 
ATGCTGCTGC CGCCCCACAG GGAGAGGCCG AGCAGTGCAA TGTACTCCAA CATCACAGAG 
AATGGACAGC CACCAAACTT TCAGAGAGCC TTGTTTCATC AGGTGATCGG TCCCTGTAAG 
CCCTGCAGTG ACCCCAACCT CTCTGTGGCT GAGAAAGTCC TCACCACTCC CAGCAGCTGG 
AGTTTGGACA GCGGCACCAG GGAGGCTCTG CCTTTCCTTT CTTCTCATGT TGGCAGCGTT 
ATGGCTCCAC CGGTTCCCCC GCGGAACGTG CCTCATGGGC AGCACCTGTC GATGCACTTT 
GACGCTTTCC ACCACCAGGT CAGCGACCTC CCTCCAGCTC TCCCTGCGCG CTCCTTAAGA 
AAGTCTCCCC TGCACCCTAT ACCTGCCTCG CCCACCAGTC CGCAGTCCGT CCTGGATGGC 
AGTAACTCCA CACTCTCAGG CAGTGCCAGC TCTGGGGTTT CATCCCTCAG TGAGAGCAAC 
TTTGGCGGCG CGACCTCCTC CTCAGACCCC GCGGCCAGCC GCACAGACAC CCTGGAGTCC 
GTCCCGAGCA GCCAGGCCTG GACCACCGAC CAGGAAGACT TGGACTCTCC TTATCAGCCC 
GTCAGGTACA GCATGTCTGA GCCAGACGTC CTGGAGGGAG TTAAATCCCC GCCTTGCCGC 
AGCAACTCTG CCCCAGGAGG AGTGAACCCG CCCCAGGTCA TCTATCAGAT CCAGTCTCAG 
GCTCCAGGTT TGGGCTTCAC CGGACCTCAG TCCCAACAGC CGGATGGACT GCAACAGCAA 
CAACACCCGT TTTACCACCA CCACTTCCAC CCACACTACA TTCCCCACAA TCCACCTCTC 
TATCATCTCC ATGAGCCCCC TCCTGCCGTA CCTCCTAAAC CCTACCTCAG AGAGGGCTGC 
ATCCCTGAGG AGGACCACCT GATCACCCAG TCTGGTCCAT CGCGCCCTCC CGTACCTCGA 
CCAATGCCGC GTAAGATCTC CCAGCCCTTA ATCGCAGCAA CCAAGGACGA ACAAGCTAAG 
GTGGCGTGGG AGCACGGCAT CAGTGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312990.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168665.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X2, mRNA.

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CloneID	OFu130680
Clone ID Related Accession (Same CDS sequence)	XM_021312990.1
Accession Version	XM_021312990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGTCCACCTG GTTTTGATAA GTCTGGCTCA 
GGAATCAACA GAGGGATCCT GGCCTCTCAC AACCCGATGA GTCCTGAGGG CTTCAAGCTC 
ATGCACCGAC ACAGTGAAGC AGGAAATCTG CTGATCCTGG CGGATGGCAT GGTGGTGGAG 
CACCCAGAGG ACTTCTATCG TATGCAGGCG AGCCCCTCCT CTTCAAGTCT TAGCTCCACC 
CACTCTGCAC CTCCTCAGAT GATAAACTCC ACCCCTCCCA CATTAAGAGT GGGCTCCCCT 
TCTCTGCCAG ACAAGTACAG ACACAATAGA GAAATGCTGA TGCTGCTGCC GCCCCACAGG 
GAGAGGCCGA GCAGTGCAAT GTACTCCAAC ATCACAGAGA ATGGACAGCC ACCAAACTTT 
CAGAGAGCCT TGTTTCATCA GGTGATCGGT CCCTGTAAGC CCTGCAGTGA CCCCAACCTC 
TCTGTGGCTG AGAAAGTCCT CACCACTCCC AGCAGCTGGA GTTTGGACAG CGGCACCAGG 
GAGGCTCTGC CTTTCCTTTC TTCTCATGTT GGCAGCGTTA TGGCTCCACC GGTTCCCCCG 
CGGAACGTGC CTCATGGGCA GCACCTGTCG ATGCACTTTG ACGCTTTCCA CCACCAGGTC 
AGCGACCTCC CTCCAGCTCT CCCTGCGCGC TCCTTAAGAA AGTCTCCCCT GCACCCTATA 
CCTGCCTCGC CCACCAGTCC GCAGTCCGTC CTGGATGGCA GTAACTCCAC ACTCTCAGGC 
AGTGCCAGCT CTGGGGTTTC ATCCCTCAGT GAGAGCAACT TTGGCGGCGC GACCTCCTCC 
TCAGACCCCG CGGCCAGCCG CACAGACACC CTGGAGTCCG TCCCGAGCAG CCAGGCCTGG 
ACCACCGACC AGGAAGACTT GGACTCTCCT TATCAGCCCG TCAGGTACAG CATGTCTGAG 
CCAGACGTCC TGGAGGGAGT TAAATCCCCG CCTTGCCGCA GCAACTCTGC CCCAGGAGGA 
GTGAACCCGC CCCAGGTCAT CTATCAGATC CAGTCTCAGG CTCCAGGTTT GGGCTTCACC 
GGACCTCAGT CCCAACAGCC GGATGGACTG CAACAGCAAC AACACCCGTT TTACCACCAC 
CACTTCCACC CACACTACAT TCCCCACAAT CCACCTCTCT ATCATCTCCA TGAGCCCCCT 
CCTGCCGTAC CTCCTAAACC CTACCTCAGA GAGGGCTGCA TCCCTGAGGA GGACCACCTG 
ATCACCCAGT CTGGTCCATC GCGCCCTCCC GTACCTCGAC CAATGCCGCG TAAGATCTCC 
CAGCCCTTAA TCGCAGCAAC CAAGGACGAA CAAGCTAAGG TGGCGTGGGA GCACGGCATC 
AGTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168665.1
CDS	185..6616
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_021312990.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312990.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTAACCCA GCTGTTTGGG CCCTATCCCA GTCTGTTGGA GAAGATTGAG 
CAGGAGACGT GGAAAGAAAC AGGAATCTCG TTTGTTACAT CGGTTACACG GTTGATGGAG 
CGACTGCTGG ACTACAGGGA CTGTATGAAG GGAGATGAGA CAGAAAACAA GAAGATTGGC 
TGCACAGTGA ACCTTCTTAA TTTCTACAAA TCGGAAATCA ACAAAGAGGA GATGTACATC 
CGTTACATCC ACAAATTGTG TGACATGCAT CTGCAGGCTG AGAACTACAC TGAGGCTGCA 
TTCACGCTGC TGTTGTACTG GGAGCTGCTG CACTGGGACG AGCGGCCAGT TAAAGAGTTC 
CTGCATTATC CTGCTCAGAC TGAGTGGCAC CGGAAGGAGG GCCTCTGCCG CAAAGTCATC 
CACTATTTCA ACAAGGGAAA GTGTTGGGAA TTTGGCATTC CTCTGTGCAG AGAACTGGCC 
TTCCAGTATG AATCTCTCTA CGACTACCAG AGTCTCAGCT GGATACGGAA AATGGAGGCA 
GCATATTATG ACAATATCAT GGAGCAGCAG CGTCTGGAGC CGGAGTTCTT CAGGGTGGGA 
TTCTACGGCA GGAAGTTTCC TTTCTTCCTC AGAAACAAAG AGTTTGTCTG CCGGGGCCAT 
GACTATGAAA GGTTAGAAGC CTTCCAACAA AGGATGCTGG GAGAATTCCC ACAAGCCATT 
GCAATGCAGC ACCCGAATCA GCCAGATGAG GCCATTCTGC AGTGTGATGC ACAGTACCTC 
CAGATTTACG CTGTGACACC GTTTCCAGAA AGCGTCACCG TCCTTCAGAT GGACAGAGTT 
CCAGACCGCA TCAAGAGCTT CTATCGAGTG AACAACGTTC GGCGATTTCG TTACGACCGG 
CCGTTCCACA AAGGACCCAA AGACAGAGAG AATGAGTTTA AGAGTCTCTG GATAGAAAGG 
ACGACTCTGA TCCTCACCCA TCCTCTGCCT GGAATTTCAT GCTGGTTTGA GGTAGAAAAG 
AGGGAACTGG TGGAGGTGAG TCCATTGGAA AACGCCATCT CTGTGGTGGA AAATAAGAAC 
CAGGAGCTGC GGACTCTGAT CAGTCAGTAC CAACACAAGC AGCTGCACGG GAACATCAAC 
CTGCTCAGCA TGACGCTGAA TGGAGTCATC GACGCCGCCG TGAACGGGGG CATCGCCAGA 
TACCAGGAGG CTTTCTTTGA TAAGGAATAC ATCACTAGTC ACCCTGAAGA CACAGAGAAG 
ATCACGCAGC TCAAAGATCT CATGCAGGAG CAGGTTCATA TCTTAGGGGT GGGCTTGGCT 
GTGCACGAGA AGTTGGTGCA CCCAGAAATG CGTCCCCTGC ACAAGAAGCT GATCGATCAG 
TTCCAGATGA TGAGGAGCAG CCTCTGCCAT GGTCCACCTG GTTTTGATAA GTCTGGCTCA 
GGAATCAACA GAGGGATCCT GGCCTCTCAC AACCCGATGA GTCCTGAGGG CTTCAAGCTC 
ATGCACCGAC ACAGTGAAGC AGGAAATCTG CTGATCCTGG CGGATGGCAT GGTGGTGGAG 
CACCCAGAGG ACTTCTATCG TATGCAGGCG AGCCCCTCCT CTTCAAGTCT TAGCTCCACC 
CACTCTGCAC CTCCTCAGAT GATAAACTCC ACCCCTCCCA CATTAAGAGT GGGCTCCCCT 
TCTCTGCCAG ACAAGTACAG ACACAATAGA GAAATGCTGA TGCTGCTGCC GCCCCACAGG 
GAGAGGCCGA GCAGTGCAAT GTACTCCAAC ATCACAGAGA ATGGACAGCC ACCAAACTTT 
CAGAGAGCCT TGTTTCATCA GGTGATCGGT CCCTGTAAGC CCTGCAGTGA CCCCAACCTC 
TCTGTGGCTG AGAAAGTCCT CACCACTCCC AGCAGCTGGA GTTTGGACAG CGGCACCAGG 
GAGGCTCTGC CTTTCCTTTC TTCTCATGTT GGCAGCGTTA TGGCTCCACC GGTTCCCCCG 
CGGAACGTGC CTCATGGGCA GCACCTGTCG ATGCACTTTG ACGCTTTCCA CCACCAGGTC 
AGCGACCTCC CTCCAGCTCT CCCTGCGCGC TCCTTAAGAA AGTCTCCCCT GCACCCTATA 
CCTGCCTCGC CCACCAGTCC GCAGTCCGTC CTGGATGGCA GTAACTCCAC ACTCTCAGGC 
AGTGCCAGCT CTGGGGTTTC ATCCCTCAGT GAGAGCAACT TTGGCGGCGC GACCTCCTCC 
TCAGACCCCG CGGCCAGCCG CACAGACACC CTGGAGTCCG TCCCGAGCAG CCAGGCCTGG 
ACCACCGACC AGGAAGACTT GGACTCTCCT TATCAGCCCG TCAGGTACAG CATGTCTGAG 
CCAGACGTCC TGGAGGGAGT TAAATCCCCG CCTTGCCGCA GCAACTCTGC CCCAGGAGGA 
GTGAACCCGC CCCAGGTCAT CTATCAGATC CAGTCTCAGG CTCCAGGTTT GGGCTTCACC 
GGACCTCAGT CCCAACAGCC GGATGGACTG CAACAGCAAC AACACCCGTT TTACCACCAC 
CACTTCCACC CACACTACAT TCCCCACAAT CCACCTCTCT ATCATCTCCA TGAGCCCCCT 
CCTGCCGTAC CTCCTAAACC CTACCTCAGA GAGGGCTGCA TCCCTGAGGA GGACCACCTG 
ATCACCCAGT CTGGTCCATC GCGCCCTCCC GTACCTCGAC CAATGCCGCG TAAGATCTCC 
CAGCCCTTAA TCGCAGCAAC CAAGGACGAA CAAGCTAAGG TGGCGTGGGA GCACGGCATC 
AGTGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312991.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168666.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X3, mRNA.

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CloneID	OFu130681
Clone ID Related Accession (Same CDS sequence)	XM_021312991.1
Accession Version	XM_021312991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTTCTGTT GGAGAAGATT GAGCAGGAGA CGTGGAAAGA AACAGGAATC 
TCGTTTGTTA CATCGGTTAC ACGGTTGATG GAGCGACTGC TGGACTACAG GGACTGTATG 
AAGGGAGATG AGACAGAAAA CAAGAAGATT GGCTGCACAG TGAACCTTCT TAATTTCTAC 
AAATCGGAAA TCAACAAAGA GGAGATGTAC ATCCGTTACA TCCACAAATT GTGTGACATG 
CATCTGCAGG CTGAGAACTA CACTGAGGCT GCATTCACGC TGCTGTTGTA CTGGGAGCTG 
CTGCACTGGG ACGAGCGGCC AGTTAAAGAG TTCCTGCATT ATCCTGCTCA GACTGAGTGG 
CACCGGAAGG AGGGCCTCTG CCGCAAAGTC ATCCACTATT TCAACAAGGG AAAGTGTTGG 
GAATTTGGCA TTCCTCTGTG CAGAGAACTG GCCTTCCAGT ATGAATCTCT CTACGACTAC 
CAGAGTCTCA GCTGGATACG GAAAATGGAG GCAGCATATT ATGACAATAT CATGGAGCAG 
CAGCGTCTGG AGCCGGAGTT CTTCAGGGTG GGATTCTACG GCAGGAAGTT TCCTTTCTTC 
CTCAGAAACA AAGAGTTTGT CTGCCGGGGC CATGACTATG AAAGGTTAGA AGCCTTCCAA 
CAAAGGATGC TGGGAGAATT CCCACAAGCC ATTGCAATGC AGCACCCGAA TCAGCCAGAT 
GAGGCCATTC TGCAGTGTGA TGCACAGTAC CTCCAGATTT ACGCTGTGAC ACCGTTTCCA 
GAAAGCGTCA CCGTCCTTCA GATGGACAGA GTTCCAGACC GCATCAAGAG CTTCTATCGA 
GTGAACAACG TTCGGCGATT TCGTTACGAC CGGCCGTTCC ACAAAGGACC CAAAGACAGA 
GAGAATGAGT TTAAGAGTCT CTGGATAGAA AGGACGACTC TGATCCTCAC CCATCCTCTG 
CCTGGAATTT CATGCTGGTT TGAGGTAGAA AAGAGGGAAC TGGTGGAGGT GAGTCCATTG 
GAAAACGCCA TCTCTGTGGT GGAAAATAAG AACCAGGAGC TGCGGACTCT GATCAGTCAG 
TACCAACACA AGCAGCTGCA CGGGAACATC AACCTGCTCA GCATGACGCT GAATGGAGTC 
ATCGACGCCG CCGTGAACGG GGGCATCGCC AGATACCAGG AGGCTTTCTT TGATAAGGAA 
TACATCACTA GTCACCCTGA AGACACAGAG AAGATCACGC AGCTCAAAGA TCTCATGCAG 
GAGCAGGTTC ATATCTTAGG GGTGGGCTTG GCTGTGCACG AGAAGTTGGT GCACCCAGAA 
ATGCGTCCCC TGCACAAGAA GCTGATCGAT CAGTTCCAGA TGATGAGGAG CAGCCTCTGC 
CATGGCTTCT ATGTTGCTTT TCAGGGTCCA CCTGGTTTTG ATAAGTCTGG CTCAGGAATC 
AACAGAGGGA TCCTGGCCTC TCACAACCCG ATGAGTCCTG AGGGCTTCAA GCTCATGCAC 
CGACACAGTG AAGCAGGAAA TCTGCTGATC CTGGCGGATG GCATGGTGGT GGAGCACCCA 
GAGGACTTCT ATCGTATGCA GGCGAGCCCC TCCTCTTCAA GTCTTAGCTC CACCCACTCT 
GCACCTCCTC AGATGATAAA CTCCACCCCT CCCACATTAA GAGTGGGCTC CCCTTCTCTG 
CCAGACAAGT ACAGACACAA TAGAGAAATG CTGATGCTGC TGCCGCCCCA CAGGGAGAGG 
CCGAGCAGTG CAATGTACTC CAACATCACA GAGAATGGAC AGCCACCAAA CTTTCAGAGA 
GCCTTGTTTC ATCAGGTGAT CGGTCCCTGT AAGCCCTGCA GTGACCCCAA CCTCTCTGTG 
GCTGAGAAAG TCCTCACCAC TCCCAGCAGC TGGAGTTTGG ACAGCGGCAC CAGGGAGGCT 
CTGCCTTTCC TTTCTTCTCA TGTTGGCAGC GTTATGGCTC CACCGGTTCC CCCGCGGAAC 
GTGCCTCATG GGCAGCACCT GTCGATGCAC TTTGACGCTT TCCACCACCA GGTCAGCGAC 
CTCCCTCCAG CTCTCCCTGC GCGCTCCTTA AGAAAGTCTC CCCTGCACCC TATACCTGCC 
TCGCCCACCA GTCCGCAGTC CGTCCTGGAT GGCAGTAACT CCACACTCTC AGGCAGTGCC 
AGCTCTGGGG TTTCATCCCT CAGTGAGAGC AACTTTGGCG GCGCGACCTC CTCCTCAGAC 
CCCGCGGCCA GCCGCACAGA CACCCTGGAG TCCGTCCCGA GCAGCCAGGC CTGGACCACC 
GACCAGGAAG ACTTGGACTC TCCTTATCAG CCCGTCAGGT ACAGCATGTC TGAGCCAGAC 
GTCCTGGAGG GAGTTAAATC CCCGCCTTGC CGCAGCAACT CTGCCCCAGG AGGAGTGAAC 
CCGCCCCAGG TCATCTATCA GATCCAGTCT CAGGCTCCAG GTTTGGGCTT CACCGGACCT 
CAGTCCCAAC AGCCGGATGG ACTGCAACAG CAACAACACC CGTTTTACCA CCACCACTTC 
CACCCACACT ACATTCCCCA CAATCCACCT CTCTATCATC TCCATGAGCC CCCTCCTGCC 
GTACCTCCTA AACCCTACCT CAGAGAGGGC TGCATCCCTG AGGAGGACCA CCTGATCACC 
CAGTCTGGTC CATCGCGCCC TCCCGTACCT CGACCAATGC CGCGTAAGAT CTCCCAGCCC 
TTAATCGCAG CAACCAAGGA CGAACAAGCT AAGGTGGCGT GGGAGCACGG CATCAGTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168666.1
CDS	185..6610
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_021312991.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312991.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGTA AGGGGTCAAC GCTGTCCGCA GTGCACCGCG 
GAGATCACTG GAGAGTACGT CTCGTGTCTC CTCTCGCTTC TTCGCCAGAT GACTGACATC 
CACTTTCAAC ACTTGATGGA AAATTTTCAA AGCAAGGACG AGCTCAAGGA GTTCTTGTTG 
AAGATTTTGT GCGTGTTTAG AAATCTGATG AAGCTCAGTA TTTTTCCCCG CGACTGGAAC 
ATCATGAGGC TGCTCACCAG CAACATCATC CTCACCACAG CTCAGCAACT TGCTCCTGCG 
CTGCACAAGA ACTTTGCTGA AACAGACTTT GACTTCAAGG TGTGGAACTC CTACTTCAGT 
CTGGCCGTTC TCTACATCAA CCAGCCGAGT TTACAGCTGG AGAACCTGAG TCCTGTGAAG 
AGGAAGAAGA TCTTAGACAA GTATGGTGAC ATGAGAGTAA TGATGACTTA TGAACTTTTC 
AACATGTGGC AGAACTTGGG AGAGAATAAA ATCCATTTCA TTCCTGGGAT GATCGGACCG 
TTCCTCGGAG TGACACTGGT CCCTCAGGTG GAGGTGAGAA ACATCATGAT TCCGATCTTC 
CACGACATGA TGGACTGGGA GCAGCGCAAA AATGGAAACT TCAAACAGGT GGAGGCTGAA 
CTAATTGACA AGTTGGACAG TTTGGTGTCG GAGGGGAAGG GTGATGAGAA CTACAGAGAG 
CTGTTTGGGC TGCTTCTGTT GGAGAAGATT GAGCAGGAGA CGTGGAAAGA AACAGGAATC 
TCGTTTGTTA CATCGGTTAC ACGGTTGATG GAGCGACTGC TGGACTACAG GGACTGTATG 
AAGGGAGATG AGACAGAAAA CAAGAAGATT GGCTGCACAG TGAACCTTCT TAATTTCTAC 
AAATCGGAAA TCAACAAAGA GGAGATGTAC ATCCGTTACA TCCACAAATT GTGTGACATG 
CATCTGCAGG CTGAGAACTA CACTGAGGCT GCATTCACGC TGCTGTTGTA CTGGGAGCTG 
CTGCACTGGG ACGAGCGGCC AGTTAAAGAG TTCCTGCATT ATCCTGCTCA GACTGAGTGG 
CACCGGAAGG AGGGCCTCTG CCGCAAAGTC ATCCACTATT TCAACAAGGG AAAGTGTTGG 
GAATTTGGCA TTCCTCTGTG CAGAGAACTG GCCTTCCAGT ATGAATCTCT CTACGACTAC 
CAGAGTCTCA GCTGGATACG GAAAATGGAG GCAGCATATT ATGACAATAT CATGGAGCAG 
CAGCGTCTGG AGCCGGAGTT CTTCAGGGTG GGATTCTACG GCAGGAAGTT TCCTTTCTTC 
CTCAGAAACA AAGAGTTTGT CTGCCGGGGC CATGACTATG AAAGGTTAGA AGCCTTCCAA 
CAAAGGATGC TGGGAGAATT CCCACAAGCC ATTGCAATGC AGCACCCGAA TCAGCCAGAT 
GAGGCCATTC TGCAGTGTGA TGCACAGTAC CTCCAGATTT ACGCTGTGAC ACCGTTTCCA 
GAAAGCGTCA CCGTCCTTCA GATGGACAGA GTTCCAGACC GCATCAAGAG CTTCTATCGA 
GTGAACAACG TTCGGCGATT TCGTTACGAC CGGCCGTTCC ACAAAGGACC CAAAGACAGA 
GAGAATGAGT TTAAGAGTCT CTGGATAGAA AGGACGACTC TGATCCTCAC CCATCCTCTG 
CCTGGAATTT CATGCTGGTT TGAGGTAGAA AAGAGGGAAC TGGTGGAGGT GAGTCCATTG 
GAAAACGCCA TCTCTGTGGT GGAAAATAAG AACCAGGAGC TGCGGACTCT GATCAGTCAG 
TACCAACACA AGCAGCTGCA CGGGAACATC AACCTGCTCA GCATGACGCT GAATGGAGTC 
ATCGACGCCG CCGTGAACGG GGGCATCGCC AGATACCAGG AGGCTTTCTT TGATAAGGAA 
TACATCACTA GTCACCCTGA AGACACAGAG AAGATCACGC AGCTCAAAGA TCTCATGCAG 
GAGCAGGTTC ATATCTTAGG GGTGGGCTTG GCTGTGCACG AGAAGTTGGT GCACCCAGAA 
ATGCGTCCCC TGCACAAGAA GCTGATCGAT CAGTTCCAGA TGATGAGGAG CAGCCTCTGC 
CATGGCTTCT ATGTTGCTTT TCAGGGTCCA CCTGGTTTTG ATAAGTCTGG CTCAGGAATC 
AACAGAGGGA TCCTGGCCTC TCACAACCCG ATGAGTCCTG AGGGCTTCAA GCTCATGCAC 
CGACACAGTG AAGCAGGAAA TCTGCTGATC CTGGCGGATG GCATGGTGGT GGAGCACCCA 
GAGGACTTCT ATCGTATGCA GGCGAGCCCC TCCTCTTCAA GTCTTAGCTC CACCCACTCT 
GCACCTCCTC AGATGATAAA CTCCACCCCT CCCACATTAA GAGTGGGCTC CCCTTCTCTG 
CCAGACAAGT ACAGACACAA TAGAGAAATG CTGATGCTGC TGCCGCCCCA CAGGGAGAGG 
CCGAGCAGTG CAATGTACTC CAACATCACA GAGAATGGAC AGCCACCAAA CTTTCAGAGA 
GCCTTGTTTC ATCAGGTGAT CGGTCCCTGT AAGCCCTGCA GTGACCCCAA CCTCTCTGTG 
GCTGAGAAAG TCCTCACCAC TCCCAGCAGC TGGAGTTTGG ACAGCGGCAC CAGGGAGGCT 
CTGCCTTTCC TTTCTTCTCA TGTTGGCAGC GTTATGGCTC CACCGGTTCC CCCGCGGAAC 
GTGCCTCATG GGCAGCACCT GTCGATGCAC TTTGACGCTT TCCACCACCA GGTCAGCGAC 
CTCCCTCCAG CTCTCCCTGC GCGCTCCTTA AGAAAGTCTC CCCTGCACCC TATACCTGCC 
TCGCCCACCA GTCCGCAGTC CGTCCTGGAT GGCAGTAACT CCACACTCTC AGGCAGTGCC 
AGCTCTGGGG TTTCATCCCT CAGTGAGAGC AACTTTGGCG GCGCGACCTC CTCCTCAGAC 
CCCGCGGCCA GCCGCACAGA CACCCTGGAG TCCGTCCCGA GCAGCCAGGC CTGGACCACC 
GACCAGGAAG ACTTGGACTC TCCTTATCAG CCCGTCAGGT ACAGCATGTC TGAGCCAGAC 
GTCCTGGAGG GAGTTAAATC CCCGCCTTGC CGCAGCAACT CTGCCCCAGG AGGAGTGAAC 
CCGCCCCAGG TCATCTATCA GATCCAGTCT CAGGCTCCAG GTTTGGGCTT CACCGGACCT 
CAGTCCCAAC AGCCGGATGG ACTGCAACAG CAACAACACC CGTTTTACCA CCACCACTTC 
CACCCACACT ACATTCCCCA CAATCCACCT CTCTATCATC TCCATGAGCC CCCTCCTGCC 
GTACCTCCTA AACCCTACCT CAGAGAGGGC TGCATCCCTG AGGAGGACCA CCTGATCACC 
CAGTCTGGTC CATCGCGCCC TCCCGTACCT CGACCAATGC CGCGTAAGAT CTCCCAGCCC 
TTAATCGCAG CAACCAAGGA CGAACAAGCT AAGGTGGCGT GGGAGCACGG CATCAGTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312992.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168667.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X4, mRNA.

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CloneID	OFu130682
Clone ID Related Accession (Same CDS sequence)	XM_021312992.1
Accession Version	XM_021312992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGGA GAGTACGTCT CGTGTCTCCT CTCGCTTCTT 
CGCCAGATGA CTGACATCCA CTTTCAACAC TTGATGGAAA ATTTTCAAAG CAAGGACGAG 
CTCAAGGAGT TCTTGTTGAA GATTTTGTGC GTGTTTAGAA ATCTGATGAA GCTCAGTATT 
TTTCCCCGCG ACTGGAACAT CATGAGGCTG CTCACCAGCA ACATCATCCT CACCACAGCT 
CAGCAACTTG CTCCTGCGCT GCACAAGAAC TTTGCTGAAA CAGACTTTGA CTTCAAGGTG 
TGGAACTCCT ACTTCAGTCT GGCCGTTCTC TACATCAACC AGCCGAGTTT ACAGCTGGAG 
AACCTGAGTC CTGTGAAGAG GAAGAAGATC TTAGACAAGT ATGGTGACAT GAGAGTAATG 
ATGACTTATG AACTTTTCAA CATGTGGCAG AACTTGGGAG AGAATAAAAT CCATTTCATT 
CCTGGGATGA TCGGACCGTT CCTCGGAGTG ACACTGGTCC CTCAGGTGGA GGTGAGAAAC 
ATCATGATTC CGATCTTCCA CGACATGATG GACTGGGAGC AGCGCAAAAA TGGAAACTTC 
AAACAGGTGG AGGCTGAACT AATTGACAAG TTGGACAGTT TGGTGTCGGA GGGGAAGGGT 
GATGAGAACT ACAGAGAGCT GTTTGGGCTG CTAACCCAGC TGTTTGGGCC CTATCCCAGT 
CTGTTGGAGA AGATTGAGCA GGAGACGTGG AAAGAAACAG GAATCTCGTT TGTTACATCG 
GTTACACGGT TGATGGAGCG ACTGCTGGAC TACAGGGACT GTATGAAGGG AGATGAGACA 
GAAAACAAGA AGATTGGCTG CACAGTGAAC CTTCTTAATT TCTACAAATC GGAAATCAAC 
AAAGAGGAGA TGTACATCCG TTACATCCAC AAATTGTGTG ACATGCATCT GCAGGCTGAG 
AACTACACTG AGGCTGCATT CACGCTGCTG TTGTACTGGG AGCTGCTGCA CTGGGACGAG 
CGGCCAGTTA AAGAGTTCCT GCATTATCCT GCTCAGACTG AGTGGCACCG GAAGGAGGGC 
CTCTGCCGCA AAGTCATCCA CTATTTCAAC AAGGGAAAGT GTTGGGAATT TGGCATTCCT 
CTGTGCAGAG AACTGGCCTT CCAGTATGAA TCTCTCTACG ACTACCAGAG TCTCAGCTGG 
ATACGGAAAA TGGAGGCAGC ATATTATGAC AATATCATGG AGCAGCAGCG TCTGGAGCCG 
GAGTTCTTCA GGGTGGGATT CTACGGCAGG AAGTTTCCTT TCTTCCTCAG AAACAAAGAG 
TTTGTCTGCC GGGGCCATGA CTATGAAAGG TTAGAAGCCT TCCAACAAAG GATGCTGGGA 
GAATTCCCAC AAGCCATTGC AATGCAGCAC CCGAATCAGC CAGATGAGGC CATTCTGCAG 
TGTGATGCAC AGTACCTCCA GATTTACGCT GTGACACCGT TTCCAGAAAG CGTCACCGTC 
CTTCAGATGG ACAGAGTTCC AGACCGCATC AAGAGCTTCT ATCGAGTGAA CAACGTTCGG 
CGATTTCGTT ACGACCGGCC GTTCCACAAA GGACCCAAAG ACAGAGAGAA TGAGTTTAAG 
AGTCTCTGGA TAGAAAGGAC GACTCTGATC CTCACCCATC CTCTGCCTGG AATTTCATGC 
TGGTTTGAGG TAGAAAAGAG GGAACTGGTG GAGGTGAGTC CATTGGAAAA CGCCATCTCT 
GTGGTGGAAA ATAAGAACCA GGAGCTGCGG ACTCTGATCA GTCAGTACCA ACACAAGCAG 
CTGCACGGGA ACATCAACCT GCTCAGCATG ACGCTGAATG GAGTCATCGA CGCCGCCGTG 
AACGGGGGCA TCGCCAGATA CCAGGAGGCT TTCTTTGATA AGGAATACAT CACTAGTCAC 
CCTGAAGACA CAGAGAAGAT CACGCAGCTC AAAGATCTCA TGCAGGAGCA GGTTCATATC 
TTAGGGGTGG GCTTGGCTGT GCACGAGAAG TTGGTGCACC CAGAAATGCG TCCCCTGCAC 
AAGAAGCTGA TCGATCAGTT CCAGATGATG AGGAGCAGCC TCTGCCATGG CTTCTATGTT 
GCTTTTCAGG GTCCACCTGG TTTTGATAAG TCTGGCTCAG GAATCAACAG AGGGATCCTG 
GCCTCTCACA ACCCGATGAG TCCTGAGGGC TTCAAGCTCA TGCACCGACA CAGTGAAGCA 
GGAAATCTGC TGATCCTGGC GGATGGCATG GTGGTGGAGC ACCCAGAGGA CTTCTATCGT 
ATGCAGGCGA GCCCCTCCTC TTCAAGTCTT AGCTCCACCC ACTCTGCACC TCCTCAGATG 
ATAAACTCCA CCCCTCCCAC ATTAAGAGTG GGCTCCCCTT CTCTGCCAGA CAAGTACAGA 
CACAATAGAG AAATGCTGAT GCTGCTGCCG CCCCACAGGG AGAGGCCGAG CAGTGCAATG 
TACTCCAACA TCACAGAGAA TGGACAGCCA CCAAACTTTC AGAGAGCCTT GTTTCATCAG 
GTGATCGGTC CCTGTAAGCC CTGCAGTGAC CCCAACCTCT CTGTGGCTGA GAAAGTCCTC 
ACCACTCCCA GCAGCTGGAG TTTGGACAGC GGCACCAGGG AGGCTCTGCC TTTCCTTTCT 
TCTCATGTTG GCAGCGTTAT GGCTCCACCG GTTCCCCCGC GGAACGTGCC TCATGGGCAG 
CACCTGTCGA TGCACTTTGA CGCTTTCCAC CACCAGGTCA GCGACCTCCC TCCAGCTCTC 
CCTGCGCGCT CCTTAAGAAA GTCTCCCCTG CACCCTATAC CTGCCTCGCC CACCAGTCCG 
CAGTCCGTCC TGGATGGCAG TAACTCCACA CTCTCAGGCA GTGCCAGCTC TGGGGTTTCA 
TCCCTCAGTG AGAGCAACTT TGGCGGCGCG ACCTCCTCCT CAGACCCCGC GGCCAGCCGC 
ACAGACACCC TGGAGTCCGT CCCGAGCAGC CAGGCCTGGA CCACCGACCA GGAAGACTTG 
GACTCTCCTT ATCAGCCCGT CAGGTACAGC ATGTCTGAGC CAGACGTCCT GGAGGGAGTT 
AAATCCCCGC CTTGCCGCAG CAACTCTGCC CCAGGAGGAG TGAACCCGCC CCAGGTCATC 
TATCAGATCC AGTCTCAGGC TCCAGGTTTG GGCTTCACCG GACCTCAGTC CCAACAGCCG 
GATGGACTGC AACAGCAACA ACACCCGTTT TACCACCACC ACTTCCACCC ACACTACATT 
CCCCACAATC CACCTCTCTA TCATCTCCAT GAGCCCCCTC CTGCCGTACC TCCTAAACCC 
TACCTCAGAG AGGGCTGCAT CCCTGAGGAG GACCACCTGA TCACCCAGTC TGGTCCATCG 
CGCCCTCCCG TACCTCGACC AATGCCGCGT AAGATCTCCC AGCCCTTAAT CGCAGCAACC 
AAGGACGAAC AAGCTAAGGT GGCGTGGGAG CACGGCATCA GTGAGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168667.1
CDS	185..6595
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQGSQPCPGPNQAPGPCIGPAGPLALVSALGQIQTPVPGQVPGSGPSNCSQAWSSNALRPASLSAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_021312992.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312992.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGGCTC CCAGCCTTGT CCCGGCCCCA ATCAGGCTCC AGGCCCCTGC 
ATAGGGCCTG CAGGCCCGTT AGCACTAGTC TCTGCCCTGG GGCAAATCCA GACCCCTGTC 
CCAGGACAGG TTCCTGGGAG CGGTCCATCT AACTGCTCTC AGGCCTGGAG CTCCAACGCT 
CTGCGGCCGG CTTCGCTGTC AGCTGCACTG TTAAACTCAT TCCCTGCCAT CTTCGACGAG 
TTGCTGCAGA TGTTCACGGT GCAGGAGGTG GCAGAGTTTG TGCGGGGCAC TTTGGGCAGC 
ATGCCGTCCA CGGTGCACAT AGGACAATCG ATGGACGTGG TGAAGCTGCA GTCCATAGCC 
CGAACCGTGG ACAGTAGGCT CTTCTCTTTC CCTGAGTCTC GAAGGATCCT GCTTCCCGTG 
GTCCTGCATC ACATCCATCT TCATCTGAGA CAACAGAAAG AGCTGCTCAT CTGCTCAGGG 
ATACTCAGCT CCATTTTCTC CATTATCAAG AGCAGCTCAC TGGACACGTC GGTGCAGGAA 
GAGGTGGAGA TGATGGTTGA GTCTCTGCTG GACGTCCTCC TGCAGACGCT TCTGTCGATC 
ATGAGCAAGA GCCAATCGCA GGAGGCGGGA GAGTACGTCT CGTGTCTCCT CTCGCTTCTT 
CGCCAGATGA CTGACATCCA CTTTCAACAC TTGATGGAAA ATTTTCAAAG CAAGGACGAG 
CTCAAGGAGT TCTTGTTGAA GATTTTGTGC GTGTTTAGAA ATCTGATGAA GCTCAGTATT 
TTTCCCCGCG ACTGGAACAT CATGAGGCTG CTCACCAGCA ACATCATCCT CACCACAGCT 
CAGCAACTTG CTCCTGCGCT GCACAAGAAC TTTGCTGAAA CAGACTTTGA CTTCAAGGTG 
TGGAACTCCT ACTTCAGTCT GGCCGTTCTC TACATCAACC AGCCGAGTTT ACAGCTGGAG 
AACCTGAGTC CTGTGAAGAG GAAGAAGATC TTAGACAAGT ATGGTGACAT GAGAGTAATG 
ATGACTTATG AACTTTTCAA CATGTGGCAG AACTTGGGAG AGAATAAAAT CCATTTCATT 
CCTGGGATGA TCGGACCGTT CCTCGGAGTG ACACTGGTCC CTCAGGTGGA GGTGAGAAAC 
ATCATGATTC CGATCTTCCA CGACATGATG GACTGGGAGC AGCGCAAAAA TGGAAACTTC 
AAACAGGTGG AGGCTGAACT AATTGACAAG TTGGACAGTT TGGTGTCGGA GGGGAAGGGT 
GATGAGAACT ACAGAGAGCT GTTTGGGCTG CTAACCCAGC TGTTTGGGCC CTATCCCAGT 
CTGTTGGAGA AGATTGAGCA GGAGACGTGG AAAGAAACAG GAATCTCGTT TGTTACATCG 
GTTACACGGT TGATGGAGCG ACTGCTGGAC TACAGGGACT GTATGAAGGG AGATGAGACA 
GAAAACAAGA AGATTGGCTG CACAGTGAAC CTTCTTAATT TCTACAAATC GGAAATCAAC 
AAAGAGGAGA TGTACATCCG TTACATCCAC AAATTGTGTG ACATGCATCT GCAGGCTGAG 
AACTACACTG AGGCTGCATT CACGCTGCTG TTGTACTGGG AGCTGCTGCA CTGGGACGAG 
CGGCCAGTTA AAGAGTTCCT GCATTATCCT GCTCAGACTG AGTGGCACCG GAAGGAGGGC 
CTCTGCCGCA AAGTCATCCA CTATTTCAAC AAGGGAAAGT GTTGGGAATT TGGCATTCCT 
CTGTGCAGAG AACTGGCCTT CCAGTATGAA TCTCTCTACG ACTACCAGAG TCTCAGCTGG 
ATACGGAAAA TGGAGGCAGC ATATTATGAC AATATCATGG AGCAGCAGCG TCTGGAGCCG 
GAGTTCTTCA GGGTGGGATT CTACGGCAGG AAGTTTCCTT TCTTCCTCAG AAACAAAGAG 
TTTGTCTGCC GGGGCCATGA CTATGAAAGG TTAGAAGCCT TCCAACAAAG GATGCTGGGA 
GAATTCCCAC AAGCCATTGC AATGCAGCAC CCGAATCAGC CAGATGAGGC CATTCTGCAG 
TGTGATGCAC AGTACCTCCA GATTTACGCT GTGACACCGT TTCCAGAAAG CGTCACCGTC 
CTTCAGATGG ACAGAGTTCC AGACCGCATC AAGAGCTTCT ATCGAGTGAA CAACGTTCGG 
CGATTTCGTT ACGACCGGCC GTTCCACAAA GGACCCAAAG ACAGAGAGAA TGAGTTTAAG 
AGTCTCTGGA TAGAAAGGAC GACTCTGATC CTCACCCATC CTCTGCCTGG AATTTCATGC 
TGGTTTGAGG TAGAAAAGAG GGAACTGGTG GAGGTGAGTC CATTGGAAAA CGCCATCTCT 
GTGGTGGAAA ATAAGAACCA GGAGCTGCGG ACTCTGATCA GTCAGTACCA ACACAAGCAG 
CTGCACGGGA ACATCAACCT GCTCAGCATG ACGCTGAATG GAGTCATCGA CGCCGCCGTG 
AACGGGGGCA TCGCCAGATA CCAGGAGGCT TTCTTTGATA AGGAATACAT CACTAGTCAC 
CCTGAAGACA CAGAGAAGAT CACGCAGCTC AAAGATCTCA TGCAGGAGCA GGTTCATATC 
TTAGGGGTGG GCTTGGCTGT GCACGAGAAG TTGGTGCACC CAGAAATGCG TCCCCTGCAC 
AAGAAGCTGA TCGATCAGTT CCAGATGATG AGGAGCAGCC TCTGCCATGG CTTCTATGTT 
GCTTTTCAGG GTCCACCTGG TTTTGATAAG TCTGGCTCAG GAATCAACAG AGGGATCCTG 
GCCTCTCACA ACCCGATGAG TCCTGAGGGC TTCAAGCTCA TGCACCGACA CAGTGAAGCA 
GGAAATCTGC TGATCCTGGC GGATGGCATG GTGGTGGAGC ACCCAGAGGA CTTCTATCGT 
ATGCAGGCGA GCCCCTCCTC TTCAAGTCTT AGCTCCACCC ACTCTGCACC TCCTCAGATG 
ATAAACTCCA CCCCTCCCAC ATTAAGAGTG GGCTCCCCTT CTCTGCCAGA CAAGTACAGA 
CACAATAGAG AAATGCTGAT GCTGCTGCCG CCCCACAGGG AGAGGCCGAG CAGTGCAATG 
TACTCCAACA TCACAGAGAA TGGACAGCCA CCAAACTTTC AGAGAGCCTT GTTTCATCAG 
GTGATCGGTC CCTGTAAGCC CTGCAGTGAC CCCAACCTCT CTGTGGCTGA GAAAGTCCTC 
ACCACTCCCA GCAGCTGGAG TTTGGACAGC GGCACCAGGG AGGCTCTGCC TTTCCTTTCT 
TCTCATGTTG GCAGCGTTAT GGCTCCACCG GTTCCCCCGC GGAACGTGCC TCATGGGCAG 
CACCTGTCGA TGCACTTTGA CGCTTTCCAC CACCAGGTCA GCGACCTCCC TCCAGCTCTC 
CCTGCGCGCT CCTTAAGAAA GTCTCCCCTG CACCCTATAC CTGCCTCGCC CACCAGTCCG 
CAGTCCGTCC TGGATGGCAG TAACTCCACA CTCTCAGGCA GTGCCAGCTC TGGGGTTTCA 
TCCCTCAGTG AGAGCAACTT TGGCGGCGCG ACCTCCTCCT CAGACCCCGC GGCCAGCCGC 
ACAGACACCC TGGAGTCCGT CCCGAGCAGC CAGGCCTGGA CCACCGACCA GGAAGACTTG 
GACTCTCCTT ATCAGCCCGT CAGGTACAGC ATGTCTGAGC CAGACGTCCT GGAGGGAGTT 
AAATCCCCGC CTTGCCGCAG CAACTCTGCC CCAGGAGGAG TGAACCCGCC CCAGGTCATC 
TATCAGATCC AGTCTCAGGC TCCAGGTTTG GGCTTCACCG GACCTCAGTC CCAACAGCCG 
GATGGACTGC AACAGCAACA ACACCCGTTT TACCACCACC ACTTCCACCC ACACTACATT 
CCCCACAATC CACCTCTCTA TCATCTCCAT GAGCCCCCTC CTGCCGTACC TCCTAAACCC 
TACCTCAGAG AGGGCTGCAT CCCTGAGGAG GACCACCTGA TCACCCAGTC TGGTCCATCG 
CGCCCTCCCG TACCTCGACC AATGCCGCGT AAGATCTCCC AGCCCTTAAT CGCAGCAACC 
AAGGACGAAC AAGCTAAGGT GGCGTGGGAG CACGGCATCA GTGAGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312993.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168668.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X5, mRNA.

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CloneID	OFu130683
Clone ID Related Accession (Same CDS sequence)	XM_021312993.1
Accession Version	XM_021312993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGCTGC ACTGTTAAAC TCATTCCCTG CCATCTTCGA CGAGTTGCTG 
CAGATGTTCA CGGTGCAGGA GGTGGCAGAG TTTGTGCGGG GCACTTTGGG CAGCATGCCG 
TCCACGGTGC ACATAGGACA ATCGATGGAC GTGGTGAAGC TGCAGTCCAT AGCCCGAACC 
GTGGACAGTA GGCTCTTCTC TTTCCCTGAG TCTCGAAGGA TCCTGCTTCC CGTGGTCCTG 
CATCACATCC ATCTTCATCT GAGACAACAG AAAGAGCTGC TCATCTGCTC AGGGATACTC 
AGCTCCATTT TCTCCATTAT CAAGAGCAGC TCACTGGACA CGTCGGTGCA GGAAGAGGTG 
GAGATGATGG TTGAGTCTCT GCTGGACGTC CTCCTGCAGA CGCTTCTGTC GATCATGAGC 
AAGAGCCAAT CGCAGGAGGC GGTAAGGGGT CAACGCTGTC CGCAGTGCAC CGCGGAGATC 
ACTGGAGAGT ACGTCTCGTG TCTCCTCTCG CTTCTTCGCC AGATGACTGA CATCCACTTT 
CAACACTTGA TGGAAAATTT TCAAAGCAAG GACGAGCTCA AGGAGTTCTT GTTGAAGATT 
TTGTGCGTGT TTAGAAATCT GATGAAGCTC AGTATTTTTC CCCGCGACTG GAACATCATG 
AGGCTGCTCA CCAGCAACAT CATCCTCACC ACAGCTCAGC AACTTGCTCC TGCGCTGCAC 
AAGAACTTTG CTGAAACAGA CTTTGACTTC AAGGTGTGGA ACTCCTACTT CAGTCTGGCC 
GTTCTCTACA TCAACCAGCC GAGTTTACAG CTGGAGAACC TGAGTCCTGT GAAGAGGAAG 
AAGATCTTAG ACAAGTATGG TGACATGAGA GTAATGATGA CTTATGAACT TTTCAACATG 
TGGCAGAACT TGGGAGAGAA TAAAATCCAT TTCATTCCTG GGATGATCGG ACCGTTCCTC 
GGAGTGACAC TGGTCCCTCA GGTGGAGGTG AGAAACATCA TGATTCCGAT CTTCCACGAC 
ATGATGGACT GGGAGCAGCG CAAAAATGGA AACTTCAAAC AGGTGGAGGC TGAACTAATT 
GACAAGTTGG ACAGTTTGGT GTCGGAGGGG AAGGGTGATG AGAACTACAG AGAGCTGTTT 
GGGCTGCTAA CCCAGCTGTT TGGGCCCTAT CCCAGTCTGT TGGAGAAGAT TGAGCAGGAG 
ACGTGGAAAG AAACAGGAAT CTCGTTTGTT ACATCGGTTA CACGGTTGAT GGAGCGACTG 
CTGGACTACA GGGACTGTAT GAAGGGAGAT GAGACAGAAA ACAAGAAGAT TGGCTGCACA 
GTGAACCTTC TTAATTTCTA CAAATCGGAA ATCAACAAAG AGGAGATGTA CATCCGTTAC 
ATCCACAAAT TGTGTGACAT GCATCTGCAG GCTGAGAACT ACACTGAGGC TGCATTCACG 
CTGCTGTTGT ACTGGGAGCT GCTGCACTGG GACGAGCGGC CAGTTAAAGA GTTCCTGCAT 
TATCCTGCTC AGACTGAGTG GCACCGGAAG GAGGGCCTCT GCCGCAAAGT CATCCACTAT 
TTCAACAAGG GAAAGTGTTG GGAATTTGGC ATTCCTCTGT GCAGAGAACT GGCCTTCCAG 
TATGAATCTC TCTACGACTA CCAGAGTCTC AGCTGGATAC GGAAAATGGA GGCAGCATAT 
TATGACAATA TCATGGAGCA GCAGCGTCTG GAGCCGGAGT TCTTCAGGGT GGGATTCTAC 
GGCAGGAAGT TTCCTTTCTT CCTCAGAAAC AAAGAGTTTG TCTGCCGGGG CCATGACTAT 
GAAAGGTTAG AAGCCTTCCA ACAAAGGATG CTGGGAGAAT TCCCACAAGC CATTGCAATG 
CAGCACCCGA ATCAGCCAGA TGAGGCCATT CTGCAGTGTG ATGCACAGTA CCTCCAGATT 
TACGCTGTGA CACCGTTTCC AGAAAGCGTC ACCGTCCTTC AGATGGACAG AGTTCCAGAC 
CGCATCAAGA GCTTCTATCG AGTGAACAAC GTTCGGCGAT TTCGTTACGA CCGGCCGTTC 
CACAAAGGAC CCAAAGACAG AGAGAATGAG TTTAAGAGTC TCTGGATAGA AAGGACGACT 
CTGATCCTCA CCCATCCTCT GCCTGGAATT TCATGCTGGT TTGAGGTAGA AAAGAGGGAA 
CTGGTGGAGG TGAGTCCATT GGAAAACGCC ATCTCTGTGG TGGAAAATAA GAACCAGGAG 
CTGCGGACTC TGATCAGTCA GTACCAACAC AAGCAGCTGC ACGGGAACAT CAACCTGCTC 
AGCATGACGC TGAATGGAGT CATCGACGCC GCCGTGAACG GGGGCATCGC CAGATACCAG 
GAGGCTTTCT TTGATAAGGA ATACATCACT AGTCACCCTG AAGACACAGA GAAGATCACG 
CAGCTCAAAG ATCTCATGCA GGAGCAGGTT CATATCTTAG GGGTGGGCTT GGCTGTGCAC 
GAGAAGTTGG TGCACCCAGA AATGCGTCCC CTGCACAAGA AGCTGATCGA TCAGTTCCAG 
ATGATGAGGA GCAGCCTCTG CCATGGCTTC TATGTTGCTT TTCAGGGTCC ACCTGGTTTT 
GATAAGTCTG GCTCAGGAAT CAACAGAGGG ATCCTGGCCT CTCACAACCC GATGAGTCCT 
GAGGGCTTCA AGCTCATGCA CCGACACAGT GAAGCAGGAA ATCTGCTGAT CCTGGCGGAT 
GGCATGGTGG TGGAGCACCC AGAGGACTTC TATCGTATGC AGGCGAGCCC CTCCTCTTCA 
AGTCTTAGCT CCACCCACTC TGCACCTCCT CAGATGATAA ACTCCACCCC TCCCACATTA 
AGAGTGGGCT CCCCTTCTCT GCCAGACAAG TACAGACACA ATAGAGAAAT GCTGATGCTG 
CTGCCGCCCC ACAGGGAGAG GCCGAGCAGT GCAATGTACT CCAACATCAC AGAGAATGGA 
CAGCCACCAA ACTTTCAGAG AGCCTTGTTT CATCAGGTGA TCGGTCCCTG TAAGCCCTGC 
AGTGACCCCA ACCTCTCTGT GGCTGAGAAA GTCCTCACCA CTCCCAGCAG CTGGAGTTTG 
GACAGCGGCA CCAGGGAGGC TCTGCCTTTC CTTTCTTCTC ATGTTGGCAG CGTTATGGCT 
CCACCGGTTC CCCCGCGGAA CGTGCCTCAT GGGCAGCACC TGTCGATGCA CTTTGACGCT 
TTCCACCACC AGGTCAGCGA CCTCCCTCCA GCTCTCCCTG CGCGCTCCTT AAGAAAGTCT 
CCCCTGCACC CTATACCTGC CTCGCCCACC AGTCCGCAGT CCGTCCTGGA TGGCAGTAAC 
TCCACACTCT CAGGCAGTGC CAGCTCTGGG GTTTCATCCC TCAGTGAGAG CAACTTTGGC 
GGCGCGACCT CCTCCTCAGA CCCCGCGGCC AGCCGCACAG ACACCCTGGA GTCCGTCCCG 
AGCAGCCAGG CCTGGACCAC CGACCAGGAA GACTTGGACT CTCCTTATCA GCCCGTCAGG 
TACAGCATGT CTGAGCCAGA CGTCCTGGAG GGAGTTAAAT CCCCGCCTTG CCGCAGCAAC 
TCTGCCCCAG GAGGAGTGAA CCCGCCCCAG GTCATCTATC AGATCCAGTC TCAGGCTCCA 
GGTTTGGGCT TCACCGGACC TCAGTCCCAA CAGCCGGATG GACTGCAACA GCAACAACAC 
CCGTTTTACC ACCACCACTT CCACCCACAC TACATTCCCC ACAATCCACC TCTCTATCAT 
CTCCATGAGC CCCCTCCTGC CGTACCTCCT AAACCCTACC TCAGAGAGGG CTGCATCCCT 
GAGGAGGACC ACCTGATCAC CCAGTCTGGT CCATCGCGCC CTCCCGTACC TCGACCAATG 
CCGCGTAAGA TCTCCCAGCC CTTAATCGCA GCAACCAAGG ACGAACAAGC TAAGGTGGCG 
TGGGAGCACG GCATCAGTGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168668.1
CDS	185..6451
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_021312993.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312993.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGCTGC ACTGTTAAAC TCATTCCCTG CCATCTTCGA CGAGTTGCTG 
CAGATGTTCA CGGTGCAGGA GGTGGCAGAG TTTGTGCGGG GCACTTTGGG CAGCATGCCG 
TCCACGGTGC ACATAGGACA ATCGATGGAC GTGGTGAAGC TGCAGTCCAT AGCCCGAACC 
GTGGACAGTA GGCTCTTCTC TTTCCCTGAG TCTCGAAGGA TCCTGCTTCC CGTGGTCCTG 
CATCACATCC ATCTTCATCT GAGACAACAG AAAGAGCTGC TCATCTGCTC AGGGATACTC 
AGCTCCATTT TCTCCATTAT CAAGAGCAGC TCACTGGACA CGTCGGTGCA GGAAGAGGTG 
GAGATGATGG TTGAGTCTCT GCTGGACGTC CTCCTGCAGA CGCTTCTGTC GATCATGAGC 
AAGAGCCAAT CGCAGGAGGC GGTAAGGGGT CAACGCTGTC CGCAGTGCAC CGCGGAGATC 
ACTGGAGAGT ACGTCTCGTG TCTCCTCTCG CTTCTTCGCC AGATGACTGA CATCCACTTT 
CAACACTTGA TGGAAAATTT TCAAAGCAAG GACGAGCTCA AGGAGTTCTT GTTGAAGATT 
TTGTGCGTGT TTAGAAATCT GATGAAGCTC AGTATTTTTC CCCGCGACTG GAACATCATG 
AGGCTGCTCA CCAGCAACAT CATCCTCACC ACAGCTCAGC AACTTGCTCC TGCGCTGCAC 
AAGAACTTTG CTGAAACAGA CTTTGACTTC AAGGTGTGGA ACTCCTACTT CAGTCTGGCC 
GTTCTCTACA TCAACCAGCC GAGTTTACAG CTGGAGAACC TGAGTCCTGT GAAGAGGAAG 
AAGATCTTAG ACAAGTATGG TGACATGAGA GTAATGATGA CTTATGAACT TTTCAACATG 
TGGCAGAACT TGGGAGAGAA TAAAATCCAT TTCATTCCTG GGATGATCGG ACCGTTCCTC 
GGAGTGACAC TGGTCCCTCA GGTGGAGGTG AGAAACATCA TGATTCCGAT CTTCCACGAC 
ATGATGGACT GGGAGCAGCG CAAAAATGGA AACTTCAAAC AGGTGGAGGC TGAACTAATT 
GACAAGTTGG ACAGTTTGGT GTCGGAGGGG AAGGGTGATG AGAACTACAG AGAGCTGTTT 
GGGCTGCTAA CCCAGCTGTT TGGGCCCTAT CCCAGTCTGT TGGAGAAGAT TGAGCAGGAG 
ACGTGGAAAG AAACAGGAAT CTCGTTTGTT ACATCGGTTA CACGGTTGAT GGAGCGACTG 
CTGGACTACA GGGACTGTAT GAAGGGAGAT GAGACAGAAA ACAAGAAGAT TGGCTGCACA 
GTGAACCTTC TTAATTTCTA CAAATCGGAA ATCAACAAAG AGGAGATGTA CATCCGTTAC 
ATCCACAAAT TGTGTGACAT GCATCTGCAG GCTGAGAACT ACACTGAGGC TGCATTCACG 
CTGCTGTTGT ACTGGGAGCT GCTGCACTGG GACGAGCGGC CAGTTAAAGA GTTCCTGCAT 
TATCCTGCTC AGACTGAGTG GCACCGGAAG GAGGGCCTCT GCCGCAAAGT CATCCACTAT 
TTCAACAAGG GAAAGTGTTG GGAATTTGGC ATTCCTCTGT GCAGAGAACT GGCCTTCCAG 
TATGAATCTC TCTACGACTA CCAGAGTCTC AGCTGGATAC GGAAAATGGA GGCAGCATAT 
TATGACAATA TCATGGAGCA GCAGCGTCTG GAGCCGGAGT TCTTCAGGGT GGGATTCTAC 
GGCAGGAAGT TTCCTTTCTT CCTCAGAAAC AAAGAGTTTG TCTGCCGGGG CCATGACTAT 
GAAAGGTTAG AAGCCTTCCA ACAAAGGATG CTGGGAGAAT TCCCACAAGC CATTGCAATG 
CAGCACCCGA ATCAGCCAGA TGAGGCCATT CTGCAGTGTG ATGCACAGTA CCTCCAGATT 
TACGCTGTGA CACCGTTTCC AGAAAGCGTC ACCGTCCTTC AGATGGACAG AGTTCCAGAC 
CGCATCAAGA GCTTCTATCG AGTGAACAAC GTTCGGCGAT TTCGTTACGA CCGGCCGTTC 
CACAAAGGAC CCAAAGACAG AGAGAATGAG TTTAAGAGTC TCTGGATAGA AAGGACGACT 
CTGATCCTCA CCCATCCTCT GCCTGGAATT TCATGCTGGT TTGAGGTAGA AAAGAGGGAA 
CTGGTGGAGG TGAGTCCATT GGAAAACGCC ATCTCTGTGG TGGAAAATAA GAACCAGGAG 
CTGCGGACTC TGATCAGTCA GTACCAACAC AAGCAGCTGC ACGGGAACAT CAACCTGCTC 
AGCATGACGC TGAATGGAGT CATCGACGCC GCCGTGAACG GGGGCATCGC CAGATACCAG 
GAGGCTTTCT TTGATAAGGA ATACATCACT AGTCACCCTG AAGACACAGA GAAGATCACG 
CAGCTCAAAG ATCTCATGCA GGAGCAGGTT CATATCTTAG GGGTGGGCTT GGCTGTGCAC 
GAGAAGTTGG TGCACCCAGA AATGCGTCCC CTGCACAAGA AGCTGATCGA TCAGTTCCAG 
ATGATGAGGA GCAGCCTCTG CCATGGCTTC TATGTTGCTT TTCAGGGTCC ACCTGGTTTT 
GATAAGTCTG GCTCAGGAAT CAACAGAGGG ATCCTGGCCT CTCACAACCC GATGAGTCCT 
GAGGGCTTCA AGCTCATGCA CCGACACAGT GAAGCAGGAA ATCTGCTGAT CCTGGCGGAT 
GGCATGGTGG TGGAGCACCC AGAGGACTTC TATCGTATGC AGGCGAGCCC CTCCTCTTCA 
AGTCTTAGCT CCACCCACTC TGCACCTCCT CAGATGATAA ACTCCACCCC TCCCACATTA 
AGAGTGGGCT CCCCTTCTCT GCCAGACAAG TACAGACACA ATAGAGAAAT GCTGATGCTG 
CTGCCGCCCC ACAGGGAGAG GCCGAGCAGT GCAATGTACT CCAACATCAC AGAGAATGGA 
CAGCCACCAA ACTTTCAGAG AGCCTTGTTT CATCAGGTGA TCGGTCCCTG TAAGCCCTGC 
AGTGACCCCA ACCTCTCTGT GGCTGAGAAA GTCCTCACCA CTCCCAGCAG CTGGAGTTTG 
GACAGCGGCA CCAGGGAGGC TCTGCCTTTC CTTTCTTCTC ATGTTGGCAG CGTTATGGCT 
CCACCGGTTC CCCCGCGGAA CGTGCCTCAT GGGCAGCACC TGTCGATGCA CTTTGACGCT 
TTCCACCACC AGGTCAGCGA CCTCCCTCCA GCTCTCCCTG CGCGCTCCTT AAGAAAGTCT 
CCCCTGCACC CTATACCTGC CTCGCCCACC AGTCCGCAGT CCGTCCTGGA TGGCAGTAAC 
TCCACACTCT CAGGCAGTGC CAGCTCTGGG GTTTCATCCC TCAGTGAGAG CAACTTTGGC 
GGCGCGACCT CCTCCTCAGA CCCCGCGGCC AGCCGCACAG ACACCCTGGA GTCCGTCCCG 
AGCAGCCAGG CCTGGACCAC CGACCAGGAA GACTTGGACT CTCCTTATCA GCCCGTCAGG 
TACAGCATGT CTGAGCCAGA CGTCCTGGAG GGAGTTAAAT CCCCGCCTTG CCGCAGCAAC 
TCTGCCCCAG GAGGAGTGAA CCCGCCCCAG GTCATCTATC AGATCCAGTC TCAGGCTCCA 
GGTTTGGGCT TCACCGGACC TCAGTCCCAA CAGCCGGATG GACTGCAACA GCAACAACAC 
CCGTTTTACC ACCACCACTT CCACCCACAC TACATTCCCC ACAATCCACC TCTCTATCAT 
CTCCATGAGC CCCCTCCTGC CGTACCTCCT AAACCCTACC TCAGAGAGGG CTGCATCCCT 
GAGGAGGACC ACCTGATCAC CCAGTCTGGT CCATCGCGCC CTCCCGTACC TCGACCAATG 
CCGCGTAAGA TCTCCCAGCC CTTAATCGCA GCAACCAAGG ACGAACAAGC TAAGGTGGCG 
TGGGAGCACG GCATCAGTGA GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312995.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168670.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X6, mRNA.

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CloneID	OFu130684
Clone ID Related Accession (Same CDS sequence)	XM_021312995.1
Accession Version	XM_021312995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGCTGC ACTGTTAAAC TCATTCCCTG CCATCTTCGA CGAGTTGCTG 
CAGATGTTCA CGGTGCAGGA GGTGGCAGAG TTTGTGCGGG GCACTTTGGG CAGCATGCCG 
TCCACGGTGC ACATAGGACA ATCGATGGAC GTGGTGAAGC TGCAGTCCAT AGCCCGAACC 
GTGGACAGTA GGCTCTTCTC TTTCCCTGAG TCTCGAAGGA TCCTGCTTCC CGTGGTCCTG 
CATCACATCC ATCTTCATCT GAGACAACAG AAAGAGCTGC TCATCTGCTC AGGGATACTC 
AGCTCCATTT TCTCCATTAT CAAGAGCAGC TCACTGGACA CGTCGGTGCA GGAAGAGGTG 
GAGATGATGG TTGAGTCTCT GCTGGACGTC CTCCTGCAGA CGCTTCTGTC GATCATGAGC 
AAGAGCCAAT CGCAGGAGGC GGTAAGGGGT CAACGCTGTC CGCAGTGCAC CGCGGAGATC 
ACTGGAGAGT ACGTCTCGTG TCTCCTCTCG CTTCTTCGCC AGATGACTGA CATCCACTTT 
CAACACTTGA TGGAAAATTT TCAAAGCAAG GACGAGCTCA AGGAGTTCTT GTTGAAGATT 
TTGTGCGTGT TTAGAAATCT GATGAAGCTC AGTATTTTTC CCCGCGACTG GAACATCATG 
AGGCTGCTCA CCAGCAACAT CATCCTCACC ACAGCTCAGC AACTTGCTCC TGCGCTGCAC 
AAGAACTTTG CTGAAACAGA CTTTGACTTC AAGGTGTGGA ACTCCTACTT CAGTCTGGCC 
GTTCTCTACA TCAACCAGCC GAGTTTACAG CTGGAGAACC TGAGTCCTGT GAAGAGGAAG 
AAGATCTTAG ACAAGTATGG TGACATGAGA GTAATGATGA CTTATGAACT TTTCAACATG 
TGGCAGAACT TGGGAGAGAA TAAAATCCAT TTCATTCCTG GGATGATCGG ACCGTTCCTC 
GGAGTGACAC TGGTCCCTCA GGTGGAGGTG AGAAACATCA TGATTCCGAT CTTCCACGAC 
ATGATGGACT GGGAGCAGCG CAAAAATGGA AACTTCAAAC AGGTGGAGGC TGAACTAATT 
GACAAGTTGG ACAGTTTGGT GTCGGAGGGG AAGGGTGATG AGAACTACAG AGAGCTGTTT 
GGGCTGCTAA CCCAGCTGTT TGGGCCCTAT CCCAGTCTGT TGGAGAAGAT TGAGCAGGAG 
ACGTGGAAAG AAACAGGAAT CTCGTTTGTT ACATCGGTTA CACGGTTGAT GGAGCGACTG 
CTGGACTACA GGGACTGTAT GAAGGGAGAT GAGACAGAAA ACAAGAAGAT TGGCTGCACA 
GTGAACCTTC TTAATTTCTA CAAATCGGAA ATCAACAAAG AGGAGATGTA CATCCGTTAC 
ATCCACAAAT TGTGTGACAT GCATCTGCAG GCTGAGAACT ACACTGAGGC TGCATTCACG 
CTGCTGTTGT ACTGGGAGCT GCTGCACTGG GACGAGCGGC CAGTTAAAGA GTTCCTGCAT 
TATCCTGCTC AGACTGAGTG GCACCGGAAG GAGGGCCTCT GCCGCAAAGT CATCCACTAT 
TTCAACAAGG GAAAGTGTTG GGAATTTGGC ATTCCTCTGT GCAGAGAACT GGCCTTCCAG 
TATGAATCTC TCTACGACTA CCAGAGTCTC AGCTGGATAC GGAAAATGGA GGCAGCATAT 
TATGACAATA TCATGGAGCA GCAGCGTCTG GAGCCGGAGT TCTTCAGGGT GGGATTCTAC 
GGCAGGAAGT TTCCTTTCTT CCTCAGAAAC AAAGAGTTTG TCTGCCGGGG CCATGACTAT 
GAAAGGTTAG AAGCCTTCCA ACAAAGGATG CTGGGAGAAT TCCCACAAGC CATTGCAATG 
CAGCACCCGA ATCAGCCAGA TGAGGCCATT CTGCAGTGTG ATGCACAGTA CCTCCAGATT 
TACGCTGTGA CACCGTTTCC AGAAAGCGTC ACCGTCCTTC AGATGGACAG AGTTCCAGAC 
CGCATCAAGA GCTTCTATCG AGTGAACAAC GTTCGGCGAT TTCGTTACGA CCGGCCGTTC 
CACAAAGGAC CCAAAGACAG AGAGAATGAG TTTAAGAGTC TCTGGATAGA AAGGACGACT 
CTGATCCTCA CCCATCCTCT GCCTGGAATT TCATGCTGGT TTGAGGTAGA AAAGAGGGAA 
CTGGTGGAGG TGAGTCCATT GGAAAACGCC ATCTCTGTGG TGGAAAATAA GAACCAGGAG 
CTGCGGACTC TGATCAGTCA GTACCAACAC AAGCAGCTGC ACGGGAACAT CAACCTGCTC 
AGCATGACGC TGAATGGAGT CATCGACGCC GCCGTGAACG GGGGCATCGC CAGATACCAG 
GAGGCTTTCT TTGATAAGGA ATACATCACT AGTCACCCTG AAGACACAGA GAAGATCACG 
CAGCTCAAAG ATCTCATGCA GGAGCAGGTT CATATCTTAG GGGTGGGCTT GGCTGTGCAC 
GAGAAGTTGG TGCACCCAGA AATGCGTCCC CTGCACAAGA AGCTGATCGA TCAGTTCCAG 
ATGATGAGGA GCAGCCTCTG CCATGGTCCA CCTGGTTTTG ATAAGTCTGG CTCAGGAATC 
AACAGAGGGA TCCTGGCCTC TCACAACCCG ATGAGTCCTG AGGGCTTCAA GCTCATGCAC 
CGACACAGTG AAGCAGGAAA TCTGCTGATC CTGGCGGATG GCATGGTGGT GGAGCACCCA 
GAGGACTTCT ATCGTATGCA GGCGAGCCCC TCCTCTTCAA GTCTTAGCTC CACCCACTCT 
GCACCTCCTC AGATGATAAA CTCCACCCCT CCCACATTAA GAGTGGGCTC CCCTTCTCTG 
CCAGACAAGT ACAGACACAA TAGAGAAATG CTGATGCTGC TGCCGCCCCA CAGGGAGAGG 
CCGAGCAGTG CAATGTACTC CAACATCACA GAGAATGGAC AGCCACCAAA CTTTCAGAGA 
GCCTTGTTTC ATCAGGTGAT CGGTCCCTGT AAGCCCTGCA GTGACCCCAA CCTCTCTGTG 
GCTGAGAAAG TCCTCACCAC TCCCAGCAGC TGGAGTTTGG ACAGCGGCAC CAGGGAGGCT 
CTGCCTTTCC TTTCTTCTCA TGTTGGCAGC GTTATGGCTC CACCGGTTCC CCCGCGGAAC 
GTGCCTCATG GGCAGCACCT GTCGATGCAC TTTGACGCTT TCCACCACCA GGTCAGCGAC 
CTCCCTCCAG CTCTCCCTGC GCGCTCCTTA AGAAAGTCTC CCCTGCACCC TATACCTGCC 
TCGCCCACCA GTCCGCAGTC CGTCCTGGAT GGCAGTAACT CCACACTCTC AGGCAGTGCC 
AGCTCTGGGG TTTCATCCCT CAGTGAGAGC AACTTTGGCG GCGCGACCTC CTCCTCAGAC 
CCCGCGGCCA GCCGCACAGA CACCCTGGAG TCCGTCCCGA GCAGCCAGGC CTGGACCACC 
GACCAGGAAG ACTTGGACTC TCCTTATCAG CCCGTCAGGT ACAGCATGTC TGAGCCAGAC 
GTCCTGGAGG GAGTTAAATC CCCGCCTTGC CGCAGCAACT CTGCCCCAGG AGGAGTGAAC 
CCGCCCCAGG TCATCTATCA GATCCAGTCT CAGGCTCCAG GTTTGGGCTT CACCGGACCT 
CAGTCCCAAC AGCCGGATGG ACTGCAACAG CAACAACACC CGTTTTACCA CCACCACTTC 
CACCCACACT ACATTCCCCA CAATCCACCT CTCTATCATC TCCATGAGCC CCCTCCTGCC 
GTACCTCCTA AACCCTACCT CAGAGAGGGC TGCATCCCTG AGGAGGACCA CCTGATCACC 
CAGTCTGGTC CATCGCGCCC TCCCGTACCT CGACCAATGC CGCGTAAGAT CTCCCAGCCC 
TTAATCGCAG CAACCAAGGA CGAACAAGCT AAGGTGGCGT GGGAGCACGG CATCAGTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168670.1
CDS	185..6430
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLTQLFGPYPSLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_021312995.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312995.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGCTGC ACTGTTAAAC TCATTCCCTG CCATCTTCGA CGAGTTGCTG 
CAGATGTTCA CGGTGCAGGA GGTGGCAGAG TTTGTGCGGG GCACTTTGGG CAGCATGCCG 
TCCACGGTGC ACATAGGACA ATCGATGGAC GTGGTGAAGC TGCAGTCCAT AGCCCGAACC 
GTGGACAGTA GGCTCTTCTC TTTCCCTGAG TCTCGAAGGA TCCTGCTTCC CGTGGTCCTG 
CATCACATCC ATCTTCATCT GAGACAACAG AAAGAGCTGC TCATCTGCTC AGGGATACTC 
AGCTCCATTT TCTCCATTAT CAAGAGCAGC TCACTGGACA CGTCGGTGCA GGAAGAGGTG 
GAGATGATGG TTGAGTCTCT GCTGGACGTC CTCCTGCAGA CGCTTCTGTC GATCATGAGC 
AAGAGCCAAT CGCAGGAGGC GGTAAGGGGT CAACGCTGTC CGCAGTGCAC CGCGGAGATC 
ACTGGAGAGT ACGTCTCGTG TCTCCTCTCG CTTCTTCGCC AGATGACTGA CATCCACTTT 
CAACACTTGA TGGAAAATTT TCAAAGCAAG GACGAGCTCA AGGAGTTCTT GTTGAAGATT 
TTGTGCGTGT TTAGAAATCT GATGAAGCTC AGTATTTTTC CCCGCGACTG GAACATCATG 
AGGCTGCTCA CCAGCAACAT CATCCTCACC ACAGCTCAGC AACTTGCTCC TGCGCTGCAC 
AAGAACTTTG CTGAAACAGA CTTTGACTTC AAGGTGTGGA ACTCCTACTT CAGTCTGGCC 
GTTCTCTACA TCAACCAGCC GAGTTTACAG CTGGAGAACC TGAGTCCTGT GAAGAGGAAG 
AAGATCTTAG ACAAGTATGG TGACATGAGA GTAATGATGA CTTATGAACT TTTCAACATG 
TGGCAGAACT TGGGAGAGAA TAAAATCCAT TTCATTCCTG GGATGATCGG ACCGTTCCTC 
GGAGTGACAC TGGTCCCTCA GGTGGAGGTG AGAAACATCA TGATTCCGAT CTTCCACGAC 
ATGATGGACT GGGAGCAGCG CAAAAATGGA AACTTCAAAC AGGTGGAGGC TGAACTAATT 
GACAAGTTGG ACAGTTTGGT GTCGGAGGGG AAGGGTGATG AGAACTACAG AGAGCTGTTT 
GGGCTGCTAA CCCAGCTGTT TGGGCCCTAT CCCAGTCTGT TGGAGAAGAT TGAGCAGGAG 
ACGTGGAAAG AAACAGGAAT CTCGTTTGTT ACATCGGTTA CACGGTTGAT GGAGCGACTG 
CTGGACTACA GGGACTGTAT GAAGGGAGAT GAGACAGAAA ACAAGAAGAT TGGCTGCACA 
GTGAACCTTC TTAATTTCTA CAAATCGGAA ATCAACAAAG AGGAGATGTA CATCCGTTAC 
ATCCACAAAT TGTGTGACAT GCATCTGCAG GCTGAGAACT ACACTGAGGC TGCATTCACG 
CTGCTGTTGT ACTGGGAGCT GCTGCACTGG GACGAGCGGC CAGTTAAAGA GTTCCTGCAT 
TATCCTGCTC AGACTGAGTG GCACCGGAAG GAGGGCCTCT GCCGCAAAGT CATCCACTAT 
TTCAACAAGG GAAAGTGTTG GGAATTTGGC ATTCCTCTGT GCAGAGAACT GGCCTTCCAG 
TATGAATCTC TCTACGACTA CCAGAGTCTC AGCTGGATAC GGAAAATGGA GGCAGCATAT 
TATGACAATA TCATGGAGCA GCAGCGTCTG GAGCCGGAGT TCTTCAGGGT GGGATTCTAC 
GGCAGGAAGT TTCCTTTCTT CCTCAGAAAC AAAGAGTTTG TCTGCCGGGG CCATGACTAT 
GAAAGGTTAG AAGCCTTCCA ACAAAGGATG CTGGGAGAAT TCCCACAAGC CATTGCAATG 
CAGCACCCGA ATCAGCCAGA TGAGGCCATT CTGCAGTGTG ATGCACAGTA CCTCCAGATT 
TACGCTGTGA CACCGTTTCC AGAAAGCGTC ACCGTCCTTC AGATGGACAG AGTTCCAGAC 
CGCATCAAGA GCTTCTATCG AGTGAACAAC GTTCGGCGAT TTCGTTACGA CCGGCCGTTC 
CACAAAGGAC CCAAAGACAG AGAGAATGAG TTTAAGAGTC TCTGGATAGA AAGGACGACT 
CTGATCCTCA CCCATCCTCT GCCTGGAATT TCATGCTGGT TTGAGGTAGA AAAGAGGGAA 
CTGGTGGAGG TGAGTCCATT GGAAAACGCC ATCTCTGTGG TGGAAAATAA GAACCAGGAG 
CTGCGGACTC TGATCAGTCA GTACCAACAC AAGCAGCTGC ACGGGAACAT CAACCTGCTC 
AGCATGACGC TGAATGGAGT CATCGACGCC GCCGTGAACG GGGGCATCGC CAGATACCAG 
GAGGCTTTCT TTGATAAGGA ATACATCACT AGTCACCCTG AAGACACAGA GAAGATCACG 
CAGCTCAAAG ATCTCATGCA GGAGCAGGTT CATATCTTAG GGGTGGGCTT GGCTGTGCAC 
GAGAAGTTGG TGCACCCAGA AATGCGTCCC CTGCACAAGA AGCTGATCGA TCAGTTCCAG 
ATGATGAGGA GCAGCCTCTG CCATGGTCCA CCTGGTTTTG ATAAGTCTGG CTCAGGAATC 
AACAGAGGGA TCCTGGCCTC TCACAACCCG ATGAGTCCTG AGGGCTTCAA GCTCATGCAC 
CGACACAGTG AAGCAGGAAA TCTGCTGATC CTGGCGGATG GCATGGTGGT GGAGCACCCA 
GAGGACTTCT ATCGTATGCA GGCGAGCCCC TCCTCTTCAA GTCTTAGCTC CACCCACTCT 
GCACCTCCTC AGATGATAAA CTCCACCCCT CCCACATTAA GAGTGGGCTC CCCTTCTCTG 
CCAGACAAGT ACAGACACAA TAGAGAAATG CTGATGCTGC TGCCGCCCCA CAGGGAGAGG 
CCGAGCAGTG CAATGTACTC CAACATCACA GAGAATGGAC AGCCACCAAA CTTTCAGAGA 
GCCTTGTTTC ATCAGGTGAT CGGTCCCTGT AAGCCCTGCA GTGACCCCAA CCTCTCTGTG 
GCTGAGAAAG TCCTCACCAC TCCCAGCAGC TGGAGTTTGG ACAGCGGCAC CAGGGAGGCT 
CTGCCTTTCC TTTCTTCTCA TGTTGGCAGC GTTATGGCTC CACCGGTTCC CCCGCGGAAC 
GTGCCTCATG GGCAGCACCT GTCGATGCAC TTTGACGCTT TCCACCACCA GGTCAGCGAC 
CTCCCTCCAG CTCTCCCTGC GCGCTCCTTA AGAAAGTCTC CCCTGCACCC TATACCTGCC 
TCGCCCACCA GTCCGCAGTC CGTCCTGGAT GGCAGTAACT CCACACTCTC AGGCAGTGCC 
AGCTCTGGGG TTTCATCCCT CAGTGAGAGC AACTTTGGCG GCGCGACCTC CTCCTCAGAC 
CCCGCGGCCA GCCGCACAGA CACCCTGGAG TCCGTCCCGA GCAGCCAGGC CTGGACCACC 
GACCAGGAAG ACTTGGACTC TCCTTATCAG CCCGTCAGGT ACAGCATGTC TGAGCCAGAC 
GTCCTGGAGG GAGTTAAATC CCCGCCTTGC CGCAGCAACT CTGCCCCAGG AGGAGTGAAC 
CCGCCCCAGG TCATCTATCA GATCCAGTCT CAGGCTCCAG GTTTGGGCTT CACCGGACCT 
CAGTCCCAAC AGCCGGATGG ACTGCAACAG CAACAACACC CGTTTTACCA CCACCACTTC 
CACCCACACT ACATTCCCCA CAATCCACCT CTCTATCATC TCCATGAGCC CCCTCCTGCC 
GTACCTCCTA AACCCTACCT CAGAGAGGGC TGCATCCCTG AGGAGGACCA CCTGATCACC 
CAGTCTGGTC CATCGCGCCC TCCCGTACCT CGACCAATGC CGCGTAAGAT CTCCCAGCCC 
TTAATCGCAG CAACCAAGGA CGAACAAGCT AAGGTGGCGT GGGAGCACGG CATCAGTGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock3 ( XM_021312996.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021168671.1 Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X7, mRNA.

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CloneID	OFu130685
Clone ID Related Accession (Same CDS sequence)	XM_021312996.1
Accession Version	XM_021312996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	dedicator of cytokinesis protein 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225057.1) and transcript sequence (GCES01127875.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 499 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGCTGC ACTGTTAAAC TCATTCCCTG CCATCTTCGA CGAGTTGCTG 
CAGATGTTCA CGGTGCAGGA GGTGGCAGAG TTTGTGCGGG GCACTTTGGG CAGCATGCCG 
TCCACGGTGC ACATAGGACA ATCGATGGAC GTGGTGAAGC TGCAGTCCAT AGCCCGAACC 
GTGGACAGTA GGCTCTTCTC TTTCCCTGAG TCTCGAAGGA TCCTGCTTCC CGTGGTCCTG 
CATCACATCC ATCTTCATCT GAGACAACAG AAAGAGCTGC TCATCTGCTC AGGGATACTC 
AGCTCCATTT TCTCCATTAT CAAGAGCAGC TCACTGGACA CGTCGGTGCA GGAAGAGGTG 
GAGATGATGG TTGAGTCTCT GCTGGACGTC CTCCTGCAGA CGCTTCTGTC GATCATGAGC 
AAGAGCCAAT CGCAGGAGGC GGTAAGGGGT CAACGCTGTC CGCAGTGCAC CGCGGAGATC 
ACTGGAGAGT ACGTCTCGTG TCTCCTCTCG CTTCTTCGCC AGATGACTGA CATCCACTTT 
CAACACTTGA TGGAAAATTT TCAAAGCAAG GACGAGCTCA AGGAGTTCTT GTTGAAGATT 
TTGTGCGTGT TTAGAAATCT GATGAAGCTC AGTATTTTTC CCCGCGACTG GAACATCATG 
AGGCTGCTCA CCAGCAACAT CATCCTCACC ACAGCTCAGC AACTTGCTCC TGCGCTGCAC 
AAGAACTTTG CTGAAACAGA CTTTGACTTC AAGGTGTGGA ACTCCTACTT CAGTCTGGCC 
GTTCTCTACA TCAACCAGCC GAGTTTACAG CTGGAGAACC TGAGTCCTGT GAAGAGGAAG 
AAGATCTTAG ACAAGTATGG TGACATGAGA GTAATGATGA CTTATGAACT TTTCAACATG 
TGGCAGAACT TGGGAGAGAA TAAAATCCAT TTCATTCCTG GGATGATCGG ACCGTTCCTC 
GGAGTGACAC TGGTCCCTCA GGTGGAGGTG AGAAACATCA TGATTCCGAT CTTCCACGAC 
ATGATGGACT GGGAGCAGCG CAAAAATGGA AACTTCAAAC AGGTGGAGGC TGAACTAATT 
GACAAGTTGG ACAGTTTGGT GTCGGAGGGG AAGGGTGATG AGAACTACAG AGAGCTGTTT 
GGGCTGCTTC TGTTGGAGAA GATTGAGCAG GAGACGTGGA AAGAAACAGG AATCTCGTTT 
GTTACATCGG TTACACGGTT GATGGAGCGA CTGCTGGACT ACAGGGACTG TATGAAGGGA 
GATGAGACAG AAAACAAGAA GATTGGCTGC ACAGTGAACC TTCTTAATTT CTACAAATCG 
GAAATCAACA AAGAGGAGAT GTACATCCGT TACATCCACA AATTGTGTGA CATGCATCTG 
CAGGCTGAGA ACTACACTGA GGCTGCATTC ACGCTGCTGT TGTACTGGGA GCTGCTGCAC 
TGGGACGAGC GGCCAGTTAA AGAGTTCCTG CATTATCCTG CTCAGACTGA GTGGCACCGG 
AAGGAGGGCC TCTGCCGCAA AGTCATCCAC TATTTCAACA AGGGAAAGTG TTGGGAATTT 
GGCATTCCTC TGTGCAGAGA ACTGGCCTTC CAGTATGAAT CTCTCTACGA CTACCAGAGT 
CTCAGCTGGA TACGGAAAAT GGAGGCAGCA TATTATGACA ATATCATGGA GCAGCAGCGT 
CTGGAGCCGG AGTTCTTCAG GGTGGGATTC TACGGCAGGA AGTTTCCTTT CTTCCTCAGA 
AACAAAGAGT TTGTCTGCCG GGGCCATGAC TATGAAAGGT TAGAAGCCTT CCAACAAAGG 
ATGCTGGGAG AATTCCCACA AGCCATTGCA ATGCAGCACC CGAATCAGCC AGATGAGGCC 
ATTCTGCAGT GTGATGCACA GTACCTCCAG ATTTACGCTG TGACACCGTT TCCAGAAAGC 
GTCACCGTCC TTCAGATGGA CAGAGTTCCA GACCGCATCA AGAGCTTCTA TCGAGTGAAC 
AACGTTCGGC GATTTCGTTA CGACCGGCCG TTCCACAAAG GACCCAAAGA CAGAGAGAAT 
GAGTTTAAGA GTCTCTGGAT AGAAAGGACG ACTCTGATCC TCACCCATCC TCTGCCTGGA 
ATTTCATGCT GGTTTGAGGT AGAAAAGAGG GAACTGGTGG AGGTGAGTCC ATTGGAAAAC 
GCCATCTCTG TGGTGGAAAA TAAGAACCAG GAGCTGCGGA CTCTGATCAG TCAGTACCAA 
CACAAGCAGC TGCACGGGAA CATCAACCTG CTCAGCATGA CGCTGAATGG AGTCATCGAC 
GCCGCCGTGA ACGGGGGCAT CGCCAGATAC CAGGAGGCTT TCTTTGATAA GGAATACATC 
ACTAGTCACC CTGAAGACAC AGAGAAGATC ACGCAGCTCA AAGATCTCAT GCAGGAGCAG 
GTTCATATCT TAGGGGTGGG CTTGGCTGTG CACGAGAAGT TGGTGCACCC AGAAATGCGT 
CCCCTGCACA AGAAGCTGAT CGATCAGTTC CAGATGATGA GGAGCAGCCT CTGCCATGGC 
TTCTATGTTG CTTTTCAGGG TCCACCTGGT TTTGATAAGT CTGGCTCAGG AATCAACAGA 
GGGATCCTGG CCTCTCACAA CCCGATGAGT CCTGAGGGCT TCAAGCTCAT GCACCGACAC 
AGTGAAGCAG GAAATCTGCT GATCCTGGCG GATGGCATGG TGGTGGAGCA CCCAGAGGAC 
TTCTATCGTA TGCAGGCGAG CCCCTCCTCT TCAAGTCTTA GCTCCACCCA CTCTGCACCT 
CCTCAGATGA TAAACTCCAC CCCTCCCACA TTAAGAGTGG GCTCCCCTTC TCTGCCAGAC 
AAGTACAGAC ACAATAGAGA AATGCTGATG CTGCTGCCGC CCCACAGGGA GAGGCCGAGC 
AGTGCAATGT ACTCCAACAT CACAGAGAAT GGACAGCCAC CAAACTTTCA GAGAGCCTTG 
TTTCATCAGG TGATCGGTCC CTGTAAGCCC TGCAGTGACC CCAACCTCTC TGTGGCTGAG 
AAAGTCCTCA CCACTCCCAG CAGCTGGAGT TTGGACAGCG GCACCAGGGA GGCTCTGCCT 
TTCCTTTCTT CTCATGTTGG CAGCGTTATG GCTCCACCGG TTCCCCCGCG GAACGTGCCT 
CATGGGCAGC ACCTGTCGAT GCACTTTGAC GCTTTCCACC ACCAGGTCAG CGACCTCCCT 
CCAGCTCTCC CTGCGCGCTC CTTAAGAAAG TCTCCCCTGC ACCCTATACC TGCCTCGCCC 
ACCAGTCCGC AGTCCGTCCT GGATGGCAGT AACTCCACAC TCTCAGGCAG TGCCAGCTCT 
GGGGTTTCAT CCCTCAGTGA GAGCAACTTT GGCGGCGCGA CCTCCTCCTC AGACCCCGCG 
GCCAGCCGCA CAGACACCCT GGAGTCCGTC CCGAGCAGCC AGGCCTGGAC CACCGACCAG 
GAAGACTTGG ACTCTCCTTA TCAGCCCGTC AGGTACAGCA TGTCTGAGCC AGACGTCCTG 
GAGGGAGTTA AATCCCCGCC TTGCCGCAGC AACTCTGCCC CAGGAGGAGT GAACCCGCCC 
CAGGTCATCT ATCAGATCCA GTCTCAGGCT CCAGGTTTGG GCTTCACCGG ACCTCAGTCC 
CAACAGCCGG ATGGACTGCA ACAGCAACAA CACCCGTTTT ACCACCACCA CTTCCACCCA 
CACTACATTC CCCACAATCC ACCTCTCTAT CATCTCCATG AGCCCCCTCC TGCCGTACCT 
CCTAAACCCT ACCTCAGAGA GGGCTGCATC CCTGAGGAGG ACCACCTGAT CACCCAGTCT 
GGTCCATCGC GCCCTCCCGT ACCTCGACCA ATGCCGCGTA AGATCTCCCA GCCCTTAATC 
GCAGCAACCA AGGACGAACA AGCTAAGGTG GCGTGGGAGC ACGGCATCAG TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021168671.1
CDS	185..6424
Translation	MWTSTEDEKIGVVICNFRGSVTQGLALEVGETVQILEKCEGWFRGFSTRKPNVKGVFPASYIHLKKAIVTNRGPHETVIPLEDPIVTEVTLTLQEWALLWKQLYVKHKVDLFYKVRHVMMELIDLRRQLLSGHLTQDQSRDVKRHITVRLDWGNEHLGLDLVPRKEFEMVDEDQISVSDLYKMHLSSRHSVQQSSTQGENSRQRHGEPCRVPVPHHLLVNLKSFTYNSIGEDTDIFFSLFDLREGKTISEKLMVRLNKNGGPKNPEKVDRLCALFTDLSNKDMKRDLYIVSQVIRTGRMLLNDSKKGPAHVQYRRPYGCAVLAMSDVLQIISELKEEKDFVLKVYTCNNENEWYQIHENIIRKSSTKYTAPSTNYGLIISLQLLRGDMEQVRRENPLIFSRGVAITRKLGFPDVIMPGDIRNDLYLTLERGDFERGGKSVQKNIEVTMYVIYADGEILKDCISLGSGEPNIPEYRSFVLYHNNSPRWSEVIKLPIPIDRFRGSHLRFEFRHCSTKDKGEKKLYGFAFTPLMREDGTTLSDESHELYVYKCDENTTFSNHALYLGLPCCKDDFNSCPNIPSSLIFQRSTKETFWISTQLSSTKLTQNVDLLALLKWKAHPERVMDILGRLRHVSGEEIVKFLQDILDTLFSILDDNTDKYGPLVFQSLVFIINLLRDSKYYHFRPVMDTYIQKHFAGALAYKELIRCLKWYMDRSAEVVRQDHIQEAMRALEYLFKFIIQSRILYSRATCGMEEEQFRTSIQELFQSIRFVLSLDSRNSETLIFTQAALLNSFPAIFDELLQMFTVQEVAEFVRGTLGSMPSTVHIGQSMDVVKLQSIARTVDSRLFSFPESRRILLPVVLHHIHLHLRQQKELLICSGILSSIFSIIKSSSLDTSVQEEVEMMVESLLDVLLQTLLSIMSKSQSQEAVRGQRCPQCTAEITGEYVSCLLSLLRQMTDIHFQHLMENFQSKDELKEFLLKILCVFRNLMKLSIFPRDWNIMRLLTSNIILTTAQQLAPALHKNFAETDFDFKVWNSYFSLAVLYINQPSLQLENLSPVKRKKILDKYGDMRVMMTYELFNMWQNLGENKIHFIPGMIGPFLGVTLVPQVEVRNIMIPIFHDMMDWEQRKNGNFKQVEAELIDKLDSLVSEGKGDENYRELFGLLLLEKIEQETWKETGISFVTSVTRLMERLLDYRDCMKGDETENKKIGCTVNLLNFYKSEINKEEMYIRYIHKLCDMHLQAENYTEAAFTLLLYWELLHWDERPVKEFLHYPAQTEWHRKEGLCRKVIHYFNKGKCWEFGIPLCRELAFQYESLYDYQSLSWIRKMEAAYYDNIMEQQRLEPEFFRVGFYGRKFPFFLRNKEFVCRGHDYERLEAFQQRMLGEFPQAIAMQHPNQPDEAILQCDAQYLQIYAVTPFPESVTVLQMDRVPDRIKSFYRVNNVRRFRYDRPFHKGPKDRENEFKSLWIERTTLILTHPLPGISCWFEVEKRELVEVSPLENAISVVENKNQELRTLISQYQHKQLHGNINLLSMTLNGVIDAAVNGGIARYQEAFFDKEYITSHPEDTEKITQLKDLMQEQVHILGVGLAVHEKLVHPEMRPLHKKLIDQFQMMRSSLCHGFYVAFQGPPGFDKSGSGINRGILASHNPMSPEGFKLMHRHSEAGNLLILADGMVVEHPEDFYRMQASPSSSSLSSTHSAPPQMINSTPPTLRVGSPSLPDKYRHNREMLMLLPPHRERPSSAMYSNITENGQPPNFQRALFHQVIGPCKPCSDPNLSVAEKVLTTPSSWSLDSGTREALPFLSSHVGSVMAPPVPPRNVPHGQHLSMHFDAFHHQVSDLPPALPARSLRKSPLHPIPASPTSPQSVLDGSNSTLSGSASSGVSSLSESNFGGATSSSDPAASRTDTLESVPSSQAWTTDQEDLDSPYQPVRYSMSEPDVLEGVKSPPCRSNSAPGGVNPPQVIYQIQSQAPGLGFTGPQSQQPDGLQQQQHPFYHHHFHPHYIPHNPPLYHLHEPPPAVPPKPYLREGCIPEEDHLITQSGPSRPPVPRPMPRKISQPLIAATKDEQAKVAWEHGISEE

Target ORF information:

RefSeq Version	XM_021312996.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus dedicator of cytokinesis 3 (dock3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021312996.1

ORF Insert Sequence:

ATGTGGACTT CCACGGAGGA CGAGAAAATC GGCGTTGTCA TCTGCAACTT CCGAGGCTCA 
GTGACTCAGG GTTTGGCTCT GGAGGTGGGA GAGACGGTGC AGATCTTGGA AAAATGTGAA 
GGGTGGTTCA GAGGATTCTC TACCAGGAAG CCGAATGTCA AGGGAGTTTT TCCGGCCAGC 
TACATTCACT TGAAGAAAGC CATTGTTACC AACAGAGGGC CTCACGAGAC AGTGATCCCC 
CTGGAAGACC CAATTGTTAC GGAGGTGACA TTGACTCTTC AGGAATGGGC CTTACTATGG 
AAACAACTCT ATGTGAAACA CAAGGTGGAT CTGTTCTACA AGGTCCGCCA CGTTATGATG 
GAGCTGATCG ACCTGAGGAG GCAGCTGCTG TCTGGTCACC TCACCCAGGA CCAGAGCAGA 
GACGTCAAGA GACACATCAC TGTCAGACTC GACTGGGGCA ATGAACACCT GGGTCTGGAC 
CTGGTGCCAA GAAAGGAGTT TGAGATGGTT GATGAAGACC AGATAAGTGT GTCAGACTTG 
TATAAAATGC ATTTGTCCAG CAGACACAGC GTCCAGCAGA GCAGCACACA GGGTGAGAAT 
AGTCGGCAGA GACATGGAGA ACCTTGCAGG GTTCCTGTAC CCCACCACCT ACTGGTCAAC 
CTGAAGAGCT TCACCTACAA CAGCATAGGC GAGGACACAG ACATATTTTT CTCTCTGTTT 
GATCTGAGGG AGGGAAAGAC CATCAGTGAG AAATTAATGG TGAGACTAAA CAAAAACGGA 
GGACCAAAGA ATCCGGAGAA GGTGGATCGG CTGTGTGCTC TGTTCACGGA CCTGAGCAAC 
AAAGATATGA AGAGAGATCT CTACATCGTA TCACAAGTTA TTCGAACAGG TCGCATGCTG 
CTGAATGACA GTAAGAAAGG TCCGGCACAT GTTCAGTACC GCAGACCATA CGGCTGCGCT 
GTTCTGGCCA TGAGTGACGT GCTGCAGATC ATCTCAGAGC TCAAAGAGGA GAAAGACTTT 
GTTCTTAAAG TCTACACGTG TAACAATGAG AATGAATGGT ACCAGATTCA TGAAAACATC 
ATCCGCAAGT CGAGCACCAA ATACACAGCT CCCAGCACCA ACTACGGACT GATCATCTCC 
CTGCAGCTGC TCAGAGGCGA CATGGAGCAG GTCCGCAGAG AGAACCCTCT CATTTTCAGC 
AGGGGGGTGG CCATCACTCG GAAACTGGGA TTCCCAGATG TCATCATGCC AGGCGACATC 
CGTAATGACC TCTACCTTAC TCTGGAGCGA GGAGACTTTG AGAGAGGAGG GAAGAGTGTT 
CAGAAAAACA TCGAAGTGAC TATGTATGTC ATCTACGCAG ATGGTGAAAT ACTGAAGGAC 
TGCATCAGTC TGGGTTCAGG CGAGCCCAAC ATCCCTGAGT ATCGCTCCTT CGTCCTCTAC 
CACAACAACA GCCCCCGCTG GAGTGAAGTG ATTAAGCTGC CGATTCCCAT CGATCGTTTC 
AGAGGCTCCC ACCTCCGCTT TGAGTTCAGA CATTGCTCCA CAAAGGATAA AGGAGAAAAA 
AAACTTTATG GCTTTGCCTT CACTCCTTTA ATGAGGGAGG ATGGAACAAC TTTATCTGAC 
GAGAGTCATG AGCTCTATGT CTACAAGTGC GATGAGAACA CAACCTTCAG TAACCACGCC 
CTCTACCTGG GCCTTCCCTG CTGTAAGGAC GACTTCAACA GCTGTCCCAA CATCCCCTCC 
AGCCTCATCT TCCAGCGAAG CACCAAGGAG ACGTTCTGGA TCTCCACGCA GCTCTCCTCC 
ACCAAGCTCA CTCAGAACGT GGACCTCCTG GCCCTGCTGA AGTGGAAGGC CCATCCTGAA 
CGGGTCATGG ACATCCTGGG GCGTCTGCGA CACGTCAGTG GTGAAGAGAT AGTCAAGTTT 
CTCCAGGATA TTCTGGACAC ATTATTCTCC ATCTTGGATG ACAACACAGA CAAATATGGA 
CCCCTTGTGT TTCAGTCATT GGTTTTTATC ATAAACTTGC TCAGGGACAG TAAATACTAC 
CACTTCAGGC CGGTGATGGA CACATATATC CAGAAACACT TTGCCGGAGC TCTGGCCTAC 
AAAGAGCTGA TTCGCTGTCT GAAGTGGTAC ATGGATCGTT CTGCCGAGGT GGTCAGACAG 
GACCACATTC AGGAAGCAAT GAGGGCTCTG GAGTATCTGT TCAAGTTTAT CATCCAGTCT 
CGGATCCTCT ACTCCCGGGC CACGTGCGGG ATGGAGGAGG AGCAGTTTCG GACCAGCATC 
CAGGAACTCT TTCAGTCCAT TCGCTTCGTC CTCAGCCTGG ACAGCCGCAA CTCGGAGACC 
CTCATATTCA CTCAGGCTGC ACTGTTAAAC TCATTCCCTG CCATCTTCGA CGAGTTGCTG 
CAGATGTTCA CGGTGCAGGA GGTGGCAGAG TTTGTGCGGG GCACTTTGGG CAGCATGCCG 
TCCACGGTGC ACATAGGACA ATCGATGGAC GTGGTGAAGC TGCAGTCCAT AGCCCGAACC 
GTGGACAGTA GGCTCTTCTC TTTCCCTGAG TCTCGAAGGA TCCTGCTTCC CGTGGTCCTG 
CATCACATCC ATCTTCATCT GAGACAACAG AAAGAGCTGC TCATCTGCTC AGGGATACTC 
AGCTCCATTT TCTCCATTAT CAAGAGCAGC TCACTGGACA CGTCGGTGCA GGAAGAGGTG 
GAGATGATGG TTGAGTCTCT GCTGGACGTC CTCCTGCAGA CGCTTCTGTC GATCATGAGC 
AAGAGCCAAT CGCAGGAGGC GGTAAGGGGT CAACGCTGTC CGCAGTGCAC CGCGGAGATC 
ACTGGAGAGT ACGTCTCGTG TCTCCTCTCG CTTCTTCGCC AGATGACTGA CATCCACTTT 
CAACACTTGA TGGAAAATTT TCAAAGCAAG GACGAGCTCA AGGAGTTCTT GTTGAAGATT 
TTGTGCGTGT TTAGAAATCT GATGAAGCTC AGTATTTTTC CCCGCGACTG GAACATCATG 
AGGCTGCTCA CCAGCAACAT CATCCTCACC ACAGCTCAGC AACTTGCTCC TGCGCTGCAC 
AAGAACTTTG CTGAAACAGA CTTTGACTTC AAGGTGTGGA ACTCCTACTT CAGTCTGGCC 
GTTCTCTACA TCAACCAGCC GAGTTTACAG CTGGAGAACC TGAGTCCTGT GAAGAGGAAG 
AAGATCTTAG ACAAGTATGG TGACATGAGA GTAATGATGA CTTATGAACT TTTCAACATG 
TGGCAGAACT TGGGAGAGAA TAAAATCCAT TTCATTCCTG GGATGATCGG ACCGTTCCTC 
GGAGTGACAC TGGTCCCTCA GGTGGAGGTG AGAAACATCA TGATTCCGAT CTTCCACGAC 
ATGATGGACT GGGAGCAGCG CAAAAATGGA AACTTCAAAC AGGTGGAGGC TGAACTAATT 
GACAAGTTGG ACAGTTTGGT GTCGGAGGGG AAGGGTGATG AGAACTACAG AGAGCTGTTT 
GGGCTGCTTC TGTTGGAGAA GATTGAGCAG GAGACGTGGA AAGAAACAGG AATCTCGTTT 
GTTACATCGG TTACACGGTT GATGGAGCGA CTGCTGGACT ACAGGGACTG TATGAAGGGA 
GATGAGACAG AAAACAAGAA GATTGGCTGC ACAGTGAACC TTCTTAATTT CTACAAATCG 
GAAATCAACA AAGAGGAGAT GTACATCCGT TACATCCACA AATTGTGTGA CATGCATCTG 
CAGGCTGAGA ACTACACTGA GGCTGCATTC ACGCTGCTGT TGTACTGGGA GCTGCTGCAC 
TGGGACGAGC GGCCAGTTAA AGAGTTCCTG CATTATCCTG CTCAGACTGA GTGGCACCGG 
AAGGAGGGCC TCTGCCGCAA AGTCATCCAC TATTTCAACA AGGGAAAGTG TTGGGAATTT 
GGCATTCCTC TGTGCAGAGA ACTGGCCTTC CAGTATGAAT CTCTCTACGA CTACCAGAGT 
CTCAGCTGGA TACGGAAAAT GGAGGCAGCA TATTATGACA ATATCATGGA GCAGCAGCGT 
CTGGAGCCGG AGTTCTTCAG GGTGGGATTC TACGGCAGGA AGTTTCCTTT CTTCCTCAGA 
AACAAAGAGT TTGTCTGCCG GGGCCATGAC TATGAAAGGT TAGAAGCCTT CCAACAAAGG 
ATGCTGGGAG AATTCCCACA AGCCATTGCA ATGCAGCACC CGAATCAGCC AGATGAGGCC 
ATTCTGCAGT GTGATGCACA GTACCTCCAG ATTTACGCTG TGACACCGTT TCCAGAAAGC 
GTCACCGTCC TTCAGATGGA CAGAGTTCCA GACCGCATCA AGAGCTTCTA TCGAGTGAAC 
AACGTTCGGC GATTTCGTTA CGACCGGCCG TTCCACAAAG GACCCAAAGA CAGAGAGAAT 
GAGTTTAAGA GTCTCTGGAT AGAAAGGACG ACTCTGATCC TCACCCATCC TCTGCCTGGA 
ATTTCATGCT GGTTTGAGGT AGAAAAGAGG GAACTGGTGG AGGTGAGTCC ATTGGAAAAC 
GCCATCTCTG TGGTGGAAAA TAAGAACCAG GAGCTGCGGA CTCTGATCAG TCAGTACCAA 
CACAAGCAGC TGCACGGGAA CATCAACCTG CTCAGCATGA CGCTGAATGG AGTCATCGAC 
GCCGCCGTGA ACGGGGGCAT CGCCAGATAC CAGGAGGCTT TCTTTGATAA GGAATACATC 
ACTAGTCACC CTGAAGACAC AGAGAAGATC ACGCAGCTCA AAGATCTCAT GCAGGAGCAG 
GTTCATATCT TAGGGGTGGG CTTGGCTGTG CACGAGAAGT TGGTGCACCC AGAAATGCGT 
CCCCTGCACA AGAAGCTGAT CGATCAGTTC CAGATGATGA GGAGCAGCCT CTGCCATGGC 
TTCTATGTTG CTTTTCAGGG TCCACCTGGT TTTGATAAGT CTGGCTCAGG AATCAACAGA 
GGGATCCTGG CCTCTCACAA CCCGATGAGT CCTGAGGGCT TCAAGCTCAT GCACCGACAC 
AGTGAAGCAG GAAATCTGCT GATCCTGGCG GATGGCATGG TGGTGGAGCA CCCAGAGGAC 
TTCTATCGTA TGCAGGCGAG CCCCTCCTCT TCAAGTCTTA GCTCCACCCA CTCTGCACCT 
CCTCAGATGA TAAACTCCAC CCCTCCCACA TTAAGAGTGG GCTCCCCTTC TCTGCCAGAC 
AAGTACAGAC ACAATAGAGA AATGCTGATG CTGCTGCCGC CCCACAGGGA GAGGCCGAGC 
AGTGCAATGT ACTCCAACAT CACAGAGAAT GGACAGCCAC CAAACTTTCA GAGAGCCTTG 
TTTCATCAGG TGATCGGTCC CTGTAAGCCC TGCAGTGACC CCAACCTCTC TGTGGCTGAG 
AAAGTCCTCA CCACTCCCAG CAGCTGGAGT TTGGACAGCG GCACCAGGGA GGCTCTGCCT 
TTCCTTTCTT CTCATGTTGG CAGCGTTATG GCTCCACCGG TTCCCCCGCG GAACGTGCCT 
CATGGGCAGC ACCTGTCGAT GCACTTTGAC GCTTTCCACC ACCAGGTCAG CGACCTCCCT 
CCAGCTCTCC CTGCGCGCTC CTTAAGAAAG TCTCCCCTGC ACCCTATACC TGCCTCGCCC 
ACCAGTCCGC AGTCCGTCCT GGATGGCAGT AACTCCACAC TCTCAGGCAG TGCCAGCTCT 
GGGGTTTCAT CCCTCAGTGA GAGCAACTTT GGCGGCGCGA CCTCCTCCTC AGACCCCGCG 
GCCAGCCGCA CAGACACCCT GGAGTCCGTC CCGAGCAGCC AGGCCTGGAC CACCGACCAG 
GAAGACTTGG ACTCTCCTTA TCAGCCCGTC AGGTACAGCA TGTCTGAGCC AGACGTCCTG 
GAGGGAGTTA AATCCCCGCC TTGCCGCAGC AACTCTGCCC CAGGAGGAGT GAACCCGCCC 
CAGGTCATCT ATCAGATCCA GTCTCAGGCT CCAGGTTTGG GCTTCACCGG ACCTCAGTCC 
CAACAGCCGG ATGGACTGCA ACAGCAACAA CACCCGTTTT ACCACCACCA CTTCCACCCA 
CACTACATTC CCCACAATCC ACCTCTCTAT CATCTCCATG AGCCCCCTCC TGCCGTACCT 
CCTAAACCCT ACCTCAGAGA GGGCTGCATC CCTGAGGAGG ACCACCTGAT CACCCAGTCT 
GGTCCATCGC GCCCTCCCGT ACCTCGACCA ATGCCGCGTA AGATCTCCCA GCCCTTAATC 
GCAGCAACCA AGGACGAACA AGCTAAGGTG GCGTGGGAGC ACGGCATCAG TGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	dock3
Entrez Gene ID	105920979
Full Name	dedicator of cytokinesis 3
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

dock3 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene

Clones

gRNAs

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