ORF » Species Summary » Danio rerio » si:dkey-253i9.4 cDNA ORF clone

si:dkey-253i9.4 cDNA ORF clone, Danio rerio(zebrafish)

The following si:dkey-253i9.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-253i9.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144234 XM_021471179.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144235 XM_021471180.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144236 XM_021471181.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144237 XM_021471182.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144238 XM_021471183.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144239 XM_021471184.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa146421 XM_005170930.4
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa146422 XM_005170931.4
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa146423 XM_005170932.4
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa146424 XM_009291106.3
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa146425 XM_009291107.2
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa146426 XM_021473893.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa146850 XM_021476314.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa146850 XM_021474583.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa05692 XM_021476305.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05692 XM_005170930.3
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05692 XM_021474578.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05693 XM_021476306.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05693 XM_021474579.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05693 XM_005170931.3
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05694 XM_021476308.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05694 XM_005170932.3
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa05694 XM_021474580.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46857 XM_021474581.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46857 XM_009291106.2
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46857 XM_021476310.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46858 XM_021476313.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46858 XM_021474582.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46858 XM_009291107.1
Latest version!		 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    si:dkey-253i9.4 ( XM_021471179.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326854.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa144234
    Clone ID Related Accession (Same CDS sequence) XM_021471179.1
    Accession Version XM_021471179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394477.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTA TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326854.1
    CDS586..5046
    Translation

    Target ORF information:

    RefSeq Version XM_021471179.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTA TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021471180.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326855.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa144235
    Clone ID Related Accession (Same CDS sequence) XM_021471180.1
    Accession Version XM_021471180.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394477.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTATCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326855.1
    CDS585..5030
    Translation

    Target ORF information:

    RefSeq Version XM_021471180.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471180.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTATCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021471181.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326856.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa144236
    Clone ID Related Accession (Same CDS sequence) XM_021471181.1
    Accession Version XM_021471181.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394477.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTATCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326856.1
    CDS586..5022
    Translation

    Target ORF information:

    RefSeq Version XM_021471181.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471181.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTATCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021471182.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326857.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa144237
    Clone ID Related Accession (Same CDS sequence) XM_021471182.1
    Accession Version XM_021471182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394477.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    ATCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326857.1
    CDS586..5007
    Translation

    Target ORF information:

    RefSeq Version XM_021471182.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    ATCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021471183.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326858.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa144238
    Clone ID Related Accession (Same CDS sequence) XM_021471183.1
    Accession Version XM_021471183.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394477.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTAT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326858.1
    CDS94..4173
    Translation

    Target ORF information:

    RefSeq Version XM_021471183.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTAT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021471184.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326859.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa144239
    Clone ID Related Accession (Same CDS sequence) XM_021471184.1
    Accession Version XM_021471184.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394477.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTA 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTAAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGACTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTAGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAAGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTT CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGTGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTATCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTATGAC AGCCGCAGCT GGGAGATGCT GTCAGACGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CGAACACAAA CAAGAACACC 
    GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCATTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326859.1
    CDS236..4222
    Translation

    Target ORF information:

    RefSeq Version XM_021471184.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021471184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTA 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTAAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGACTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTAGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAAGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTT CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGTGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTATCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTATGAC AGCCGCAGCT GGGAGATGCT GTCAGACGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CGAACACAAA CAAGAACACC 
    GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCATTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_005170930.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170987.2 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa146421
    Clone ID Related Accession (Same CDS sequence) XM_005170930.4
    Accession Version XM_005170930.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395211.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170930.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTCCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGA GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTA TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA CGACAGCCGC AGCTGGGAGA TGCTGTCAGA TGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCAAACA CAAACAAGAA CACCGTGAGT 
    CGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCACT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170987.2
    CDS590..5050
    Translation

    Target ORF information:

    RefSeq Version XM_005170930.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005170930.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTCCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGA GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTA TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA CGACAGCCGC AGCTGGGAGA TGCTGTCAGA TGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCAAACA CAAACAAGAA CACCGTGAGT 
    CGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCACT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_005170931.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170988.2 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa146422
    Clone ID Related Accession (Same CDS sequence) XM_005170931.4
    Accession Version XM_005170931.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395211.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTCC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGAGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTATCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTACGACA GCCGCAGCTG GGAGATGCTG TCAGATGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC AAACACAAAC AAGAACACCG TGAGTCGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCACTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170988.2
    CDS590..5035
    Translation

    Target ORF information:

    RefSeq Version XM_005170931.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005170931.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTCC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGAGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTATCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTACGACA GCCGCAGCTG GGAGATGCTG TCAGATGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC AAACACAAAC AAGAACACCG TGAGTCGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCACTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_005170932.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170989.2 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa146423
    Clone ID Related Accession (Same CDS sequence) XM_005170932.4
    Accession Version XM_005170932.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395211.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTCCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGAGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTATCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTACGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGATGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CAAACACAAA CAAGAACACC GTGAGTCGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCACTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170989.2
    CDS591..5027
    Translation

    Target ORF information:

    RefSeq Version XM_005170932.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005170932.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTCCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGAGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTATCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTACGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGATGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CAAACACAAA CAAGAACACC GTGAGTCGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCACTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_009291106.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289381.2 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa146424
    Clone ID Related Accession (Same CDS sequence) XM_009291106.3
    Accession Version XM_009291106.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395211.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTCC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    AGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    ATCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ACGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ATGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCAAAC 
    ACAAACAAGA ACACCGTGAG TCGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAC TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289381.2
    CDS590..5011
    Translation

    Target ORF information:

    RefSeq Version XM_009291106.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009291106.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATA 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTGAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT TAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA TTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTGGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AGGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTCC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    AGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    ATCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ACGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ATGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCAAAC 
    ACAAACAAGA ACACCGTGAG TCGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAC TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_009291107.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289382.2 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa146425
    Clone ID Related Accession (Same CDS sequence) XM_009291107.2
    Accession Version XM_009291107.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395211.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATAA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTGAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTT 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAT TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTGGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA GGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTCCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGAG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTAT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAC 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAT GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCAAACAC AAACAAGAAC ACCGTGAGTC GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCACTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289382.2
    CDS94..4173
    Translation

    Target ORF information:

    RefSeq Version XM_009291107.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009291107.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATAA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTGAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTT 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAT TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTGGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA GGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTCCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGAG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTAT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAC 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAT GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCAAACAC AAACAAGAAC ACCGTGAGTC GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCACTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021473893.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329568.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa146426
    Clone ID Related Accession (Same CDS sequence) XM_021473893.1
    Accession Version XM_021473893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395211.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTG 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTTAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGATTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTGGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAGGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTC CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGAGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTATCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTACGAC AGCCGCAGCT GGGAGATGCT GTCAGATGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CAAACACAAA CAAGAACACC 
    GTGAGTCGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCACTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329568.1
    CDS139..4125
    Translation

    Target ORF information:

    RefSeq Version XM_021473893.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021473893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTG 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTTAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGATTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTGGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAGGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTC CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGAGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTATCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTACGAC AGCCGCAGCT GGGAGATGCT GTCAGATGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CAAACACAAA CAAGAACACC 
    GTGAGTCGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCACTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021476314.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331989.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa146850
    Clone ID Related Accession (Same CDS sequence) XM_021476314.1 , XM_021474583.1
    Accession Version XM_021476314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTA 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTAAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGACTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTAGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAAGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTT CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGTGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTCTCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTATGAC AGCCGCAGCT GGGAGATGCT GTCAGACGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CGAACACAAA CAAGAACACC 
    GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCATTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331989.1
    CDS965..4951
    Translation

    Target ORF information:

    RefSeq Version XM_021476314.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021476314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTA 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTAAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGACTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTAGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAAGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTT CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGTGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTCTCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTATGAC AGCCGCAGCT GGGAGATGCT GTCAGACGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CGAACACAAA CAAGAACACC 
    GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCATTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021474583.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330258.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa146850
    Clone ID Related Accession (Same CDS sequence) XM_021476314.1 , XM_021474583.1
    Accession Version XM_021474583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTA 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTAAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGACTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTAGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAAGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTT CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGTGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTCTCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTATGAC AGCCGCAGCT GGGAGATGCT GTCAGACGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CGAACACAAA CAAGAACACC 
    GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCATTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330258.1
    CDS965..4951
    Translation

    Target ORF information:

    RefSeq Version XM_021474583.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTTGGGC ATTCAGTATT GCAGGCTATG AGACAGCTAG TTCTGTCTCA TGGCTATGGA 
    GTCAGGGGAC AGGAGAGCAG GACTCTCCCA ACCGACTTCT CAAAGCTTCA CCCCTCCGAC 
    AGTTGCCACT CTCATATGAC TTCCAGCCAA TCACGTTCCA GCATCGCCAG TGATTCAGGA 
    AGCAGCAGCC TATCGGATAT TTACCAGGCC ACTGAGAGCG AGTGTGGAGA CATGGATCTA 
    AGTGGGCTTC CTGAAACAGC AGTGGACTCA GACGAGGATG ATGATGATGA AGACGTAGGA 
    AGGGGAACGG ACCATCTTAT GAGTCGGGAT ATTGTGAGAG ACTGCCTTGA AAAAGACCCT 
    ATGGACCGTA CAGATGATGA CATTGAGCAA TTGCTGGAGT TTGTTCAGCA GCTTCCAGCA 
    TTTGCCAGTC TGAGTGTTTC GGTGCGAAGG GAACTCTGTG CTGTTATGGT GTTTGCGGTT 
    GTGGAACGTG CTGGAACTAT AGTCCTAAAC CATGGTGAAG AGCTGGACTC ATGGTCAGTC 
    ATTCTGAATG GAGCAGTAGA GGTGATATAC CTGGACGGCC GCAGTGAGAG TGTGTGTATG 
    GGTGGAAGTT TTGGTGTCTG CCCATCAATG GAGAAACAAC TGATGACTGG AGTAATGAGA 
    ACCAAAGTTG ATGACTGTCA GTTTGTGTGC ATTGCCCAGC AGGACTATTG CTGCATTCTG 
    AATCAGGTTG AGCAGAACAC ACAGCGAGTA GAGGAGGAAG GGGAAATCGT CATGGTCAAA 
    GAACATCGAG AACTCGACAG GACGGGCACA CGCAAGGGAC ACATTGTTGT CAAGGGTACT 
    GCTGAGAGGC TGATGCAGCA CTTAGTGGAG GACCATTCAA TCGTCGACCC CACATATATT 
    GAGGACTTTC TCTTAACCTA TCGTACCTTC CTGCCCAGTC CTCTGATTCT GGGACAGAGA 
    CTTCTAGACT GGTTTAATGA CCCCTGTTTA CGGGACAAGG TAACCCGGGT TGTGCTCTTA 
    TGGGTTAACA ATCATTTTAG TGATTTTGAA GGAGACCCTG ACATGACCAG TTTTTTGGAG 
    GAGTTTCAAA GCAACCTGGA AAGAGAGAAA ATGACCGGTC AGTTGAGGTT GCTCAACATC 
    GCTTGTGCAG CAAAAGCCAA ACCCAGAGTG ATAACTGTAA CCAGGACAGC TCGAGAGATC 
    CCCCTCCCCT TTACCCTTAT TGGAGGAGCT GAACGGGGGA CACGCCTCTT CATCAGCAGT 
    GTGGAGGTTG GCAGCAAAGC AGAGGAGGCA GGGCTTAAGC GTGGAGACCA GATATTGGAG 
    GTGAACGGAC AGACGTTTGA GAATGTGCAG TTGTCGAAAG CGACTGAAAT TCTCAGGAAC 
    AACATACAGC TCTCTATGAG TGTCAAAACC AACCTACTTG TCTTCAAGGA GTTGGTGGCA 
    AGGGTGGCCG AGGAAAGGAA AAACGGCGTT CCTCATCTTC CAAAGATTGG TGACGGAAAG 
    AAAAACTGTC GATATTCTAT ACCTGAACTT GGGGCTGGTT CTGATGTTAT AGGTCAAGAA 
    AAAATCAGCA AGAAGGCAAA AGCACATACA GTGGGTGGCC GAAACAAACT GATGAAGATT 
    CTAGACAAGA CACGCATGAG CATCCTACCT CAGAAACCTT ACAGTGACCT CGGGTTGGGG 
    CAGACTCAGG ATGACAGCAT TGTGGGATTA CGGCAGAACA AACAGACTCC ACCCACTATG 
    CCAGTGAGCG GAACTCTCTC ATCCAGTAAC CCTGACCTGG CACAAGGCCA GCAACGCATC 
    ATCGACTACA GTACACAGCC ACCAGCACCT GGATTCACTC AGTTGCAAGA CCAGGTGCTG 
    CGCATTTTCA AGGCAGATCA GCAAAGCCGT TACTTACTGG CCAATAGAGA CACCACAGCC 
    AGAGAAGCTG CCAATCTAGC CATAAAAGAG TTTGGCTTGT CTGCGAGTCC CGAAGCTTTT 
    TCGCTCTGTG AAGTTTCAGT CACACAGGAA GGAGTTATCA AGCAGAGACG ACTGCCTGAT 
    CAACTGTCCA AACTGGCTGA CAGGATCCAA CTGAGTGCCA GGTACTATCT AAAGAGCAAT 
    ATGGAAACCG AGACGCTGTG TTCGGATGTG GAAGCACTGG AGATGCAGAG AGAATCTGTG 
    GTGCCCCTGT TGTCTTTGAG CTCAATGGAG ATAGCCAATC AGCTTTCAGC ACGCAACTAT 
    CTGCTCTTCT CCTGCATCGA ACCGACAGAT TACATCAATG ACCTCTTTAA GCTTCACCCA 
    CAAGAGCCTC CCACAAACCT GCGCAACTTT GAGGGTCTGG TGAACCAGGA GACTTTCTGG 
    GTCGCCACAG AGATTGTTCA GGAAACCAAC CTGGCCAAAA GAGTGAAGAT CATCAAGCAC 
    TTTATTAAGA TTGCTCTTCA CTGCCGCGAC TGCAAGAACT TCAACTCTAT GTTCGCCATT 
    ATCAGTGGGT TCAATCTGGC TCCTGTCTCT CGGTTGCGAT CTACATGGGA ACGGTTGCCT 
    GGAAAATACG AGAAGCTTTT GGCTGAATTA CAGGATGTTT TTGACCCTTC TCGCAACATG 
    GCCAAATATC GCAACCTTTT GAACAAACAC AACCTTCAGC CGCCGGTGAT CCCTCTGTTC 
    CCCGTCATCA AGAAAGACCT CACCTTCCTT CATGAGGGCA ACAACTCAGA GGTGGACGGT 
    CTGGTGAATT TTGAGAAGTT GAGGATGATC GCACGAGAGA TCAGACACGT GGTTCGAATG 
    GCTTCCATCA CCATGGACCC TGCATTGCTT TTTAAGACTA GGAAGAAAAA GTGGAGGAGT 
    CTTGGATCTC TAAGTCAGGT GAGCAGCTCT TCTCTGTCTG ACATTGGAAC GATGGGTGGA 
    AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC AGTGCTAAGA AACTGTATGA GGTGGAGATG 
    ATGAGCAGAC GTGTGCGCCA GTATCTGGAC ACGCACTCAA ACGAGTGCAA CGAGGACACA 
    CTTCATGAGC TCTCCATGAC CTGTGAGCCT GCCAACAGCT CCACACTGAA GCACGCTGGT 
    GAGAGGAGAC GGATTGATGC ATCTCCTGCT GGTCAGAGAA GTGCCAGTCC TCAACAAACG 
    AATGGAAATC ATGGTCGCAA GAAGTCAATT AGCAAAGAGT TACCATTCGA GGGCCGAGAC 
    AGACAACGAA AGCTCCCAGA GGACAGCCAA TCAAGCACTT CATCTCTCCT CTCCTCTCCA 
    CGTAATTCAC CTCAGAGCAC ACCTAGAAAA GGCCCACAGT TAGTGGTAAG CGAGGTATCA 
    GATCAGATGA GTCTGTTAAG CTCCTCTTCC TCCGAACTCC TCATGGTTGA TGACCACACA 
    CACACTCAGC CCCATTCACT TGCACATCCA CTCAGTGCAC GCAGGGCTGA GCCAGATCAA 
    TTAAGCCTGG GGTCCTACTC CTTGACTCAG GATCAGTGTG ACCGCGCATC ACTGGACGCA 
    GCAGACAGCG GTCGTGGCAG CTGGACATCC TGCTCGAGTG GATCCCATGA CAATATCCAA 
    AGTATTCCTC AGCGTGTGGG ATATTATGAC AGCCGCAGCT GGGAGATGCT GTCAGACGGG 
    ATGGGGCGAA GTCACATGAC CACTCCCACC GGCTACTGGG CTGATGAACT GGAAGGAGAC 
    ACAGGAACGA TAAAACGCAG AGGTGGAAAA GACGAAACTC CGAACACAAA CAAGAACACC 
    GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG CCGCCGCCCA CACCACCGGG TTACACTGCT 
    GTCTCATTGG CTGAGGCAGA CACCAACAGT CATGCTCATG GGCGACGCCC ACCAGACTAC 
    AGCGCGGCTC TGCAGAGATC TCGATTCCTT AAAAGGACAT GCGATCCTCC TCCATTGCAC 
    TCGTCCAGCA GATTACCACC GTACAGCCAC TGCCAGACTC CACGACGATC ACAAGCAGAT 
    GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021476305.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331980.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa05692
    Clone ID Related Accession (Same CDS sequence) XM_021476305.1 , XM_005170930.3 , XM_021474578.1
    Accession Version XM_021476305.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTC TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331980.1
    CDS589..5049
    Translation

    Target ORF information:

    RefSeq Version XM_021476305.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021476305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTC TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_005170930.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170987.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa05692
    Clone ID Related Accession (Same CDS sequence) XM_021476305.1 , XM_005170930.3 , XM_021474578.1
    Accession Version XM_005170930.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170930.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTC TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170987.1
    CDS609..5069
    Translation

    Target ORF information:

    RefSeq Version XM_005170930.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005170930.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTC TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021474578.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330253.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa05692
    Clone ID Related Accession (Same CDS sequence) XM_021476305.1 , XM_005170930.3 , XM_021474578.1
    Accession Version XM_021474578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTC TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330253.1
    CDS589..5049
    Translation

    Target ORF information:

    RefSeq Version XM_021474578.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGGAAGA AAAAGTGGAG GAGTCTTGGA 
    TCTCTAAGTC AGGTGAGCAG CTCTTCTCTG TCTGACATTG GAACGATGGG TGGAAAGAGA 
    AAGGGCAGGC GGAGCTCTTT CCTCAGTGCT AAGAAACTGT ATGAGGTGGA GATGATGAGC 
    AGACGTGTGC GCCAGTATCT GGACACGCAC TCAAACGAGT GCAACGAGGA CACACTTCAT 
    GAGCTCTCCA TGACCTGTGA GCCTGCCAAC AGCTCCACAC TGAAGCACGC TGGTGAGAGG 
    AGACGGATTG ATGCATCTCC TGCTGGTCAG AGAAGTGCCA GTCCTCAACA AACGAATGGA 
    AATCATGGTC GCAAGAAGTC AATTAGCAAA GAGTTACCAT TCGAGGGCCG AGACAGACAA 
    CGAAAGCTCC CAGAGGACAG CCAATCAAGC ACTTCATCTC TCCTCTCCTC TCCACGTAAT 
    TCACCTCAGA GCACACCTAG AAAAGGCCCA CAGTTAGTGG TAAGCGAGGT ATCAGATCAG 
    ATGAGTCTGT TAAGCTCCTC TTCCTCCGAA CTCCTCATGG TTGATGACCA CACACACACT 
    CAGCCCCATT CACTTGCACA TCCACTCAGT GCACGCAGGG CTGAGCCAGA TCAATTAAGC 
    CTGGGGTCCT ACTCCTTGAC TCAGGATCAG TGTGACCGCG CATCACTGGA CGCAGCAGAC 
    AGCGGTCGTG GCAGCTGGAC ATCCTGCTCG AGTGGATCCC ATGACAATAT CCAAAGTATT 
    CCTCAGCGTG TGGGATATTA TGACAGCCGC AGCTGGGAGA TGCTGTCAGA CGGGATGGGG 
    CGAAGTCACA TGACCACTCC CACCGGCTAC TGGGCTGATG AACTGGAAGG AGACACAGGA 
    ACGATAAAAC GCAGAGGTGG AAAAGACGAA ACTCCGAACA CAAACAAGAA CACCGTGAGT 
    AGGAGGGAGG GGCATTTCAG AGAGCCGCCG CCCACACCAC CGGGTTACAC TGCTGTCTCA 
    TTGGCTGAGG CAGACACCAA CAGTCATGCT CATGGGCGAC GCCCACCAGA CTACAGCGCG 
    GCTCTGCAGA GATCTCGATT CCTTAAAAGG ACATGCGATC CTCCTCCATT GCACTCGTCC 
    AGCAGATTAC CACCGTACAG CCACTGCCAG ACTCCACGAC GATCACAAGC AGATGAAAGC 
    GAACAAATAT CTGCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021474579.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330254.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa05693
    Clone ID Related Accession (Same CDS sequence) XM_021474579.1 , XM_005170931.3 , XM_021476306.1
    Accession Version XM_021474579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTCTCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330254.1
    CDS589..5034
    Translation

    Target ORF information:

    RefSeq Version XM_021474579.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTCTCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_005170931.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170988.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa05693
    Clone ID Related Accession (Same CDS sequence) XM_021474579.1 , XM_005170931.3 , XM_021476306.1
    Accession Version XM_005170931.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTCTCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170988.1
    CDS608..5053
    Translation

    Target ORF information:

    RefSeq Version XM_005170931.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005170931.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTCTCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021476306.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331981.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa05693
    Clone ID Related Accession (Same CDS sequence) XM_021474579.1 , XM_005170931.3 , XM_021476306.1
    Accession Version XM_021476306.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTCTCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331981.1
    CDS588..5033
    Translation

    Target ORF information:

    RefSeq Version XM_021476306.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021476306.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG GAAGAAAAAG TGGAGGAGTC TTGGATCTCT AAGTCAGGTG 
    AGCAGCTCTT CTCTGTCTGA CATTGGAACG ATGGGTGGAA AGAGAAAGGG CAGGCGGAGC 
    TCTTTCCTCA GTGCTAAGAA ACTGTATGAG GTGGAGATGA TGAGCAGACG TGTGCGCCAG 
    TATCTGGACA CGCACTCAAA CGAGTGCAAC GAGGACACAC TTCATGAGCT CTCCATGACC 
    TGTGAGCCTG CCAACAGCTC CACACTGAAG CACGCTGGTG AGAGGAGACG GATTGATGCA 
    TCTCCTGCTG GTCAGAGAAG TGCCAGTCCT CAACAAACGA ATGGAAATCA TGGTCGCAAG 
    AAGTCAATTA GCAAAGAGTT ACCATTCGAG GGCCGAGACA GACAACGAAA GCTCCCAGAG 
    GACAGCCAAT CAAGCACTTC ATCTCTCCTC TCCTCTCCAC GTAATTCACC TCAGAGCACA 
    CCTAGAAAAG GCCCACAGTT AGTGGTAAGC GAGGTATCAG ATCAGATGAG TCTGTTAAGC 
    TCCTCTTCCT CCGAACTCCT CATGGTTGAT GACCACACAC ACACTCAGCC CCATTCACTT 
    GCACATCCAC TCAGTGCACG CAGGGCTGAG CCAGATCAAT TAAGCCTGGG GTCCTACTCC 
    TTGACTCAGG ATCAGTGTGA CCGCGCATCA CTGGACGCAG CAGACAGCGG TCGTGGCAGC 
    TGGACATCCT GCTCGAGTGG ATCCCATGAC AATATCCAAA GTATTCCTCA GCGTGTGGGA 
    TATTATGACA GCCGCAGCTG GGAGATGCTG TCAGACGGGA TGGGGCGAAG TCACATGACC 
    ACTCCCACCG GCTACTGGGC TGATGAACTG GAAGGAGACA CAGGAACGAT AAAACGCAGA 
    GGTGGAAAAG ACGAAACTCC GAACACAAAC AAGAACACCG TGAGTAGGAG GGAGGGGCAT 
    TTCAGAGAGC CGCCGCCCAC ACCACCGGGT TACACTGCTG TCTCATTGGC TGAGGCAGAC 
    ACCAACAGTC ATGCTCATGG GCGACGCCCA CCAGACTACA GCGCGGCTCT GCAGAGATCT 
    CGATTCCTTA AAAGGACATG CGATCCTCCT CCATTGCACT CGTCCAGCAG ATTACCACCG 
    TACAGCCACT GCCAGACTCC ACGACGATCA CAAGCAGATG AAAGCGAACA AATATCTGCA 
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021476308.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331983.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa05694
    Clone ID Related Accession (Same CDS sequence) XM_021476308.1 , XM_021474580.1 , XM_005170932.3
    Accession Version XM_021476308.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTCTCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331983.1
    CDS589..5025
    Translation

    Target ORF information:

    RefSeq Version XM_021476308.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021476308.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTCTCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021474580.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330255.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa05694
    Clone ID Related Accession (Same CDS sequence) XM_021476308.1 , XM_021474580.1 , XM_005170932.3
    Accession Version XM_021474580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTCTCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330255.1
    CDS589..5025
    Translation

    Target ORF information:

    RefSeq Version XM_021474580.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTCTCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_005170932.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170989.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa05694
    Clone ID Related Accession (Same CDS sequence) XM_021476308.1 , XM_021474580.1 , XM_005170932.3
    Accession Version XM_005170932.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTCTCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170989.1
    CDS609..5045
    Translation

    Target ORF information:

    RefSeq Version XM_005170932.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005170932.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGC ACCTGGATTC ACTCAGTTGC AAGACCAGGT GCTGCGCATT 
    TTCAAGGCAG ATCAGCAAAG CCGTTACTTA CTGGCCAATA GAGACACCAC AGCCAGAGAA 
    GCTGCCAATC TAGCCATAAA AGAGTTTGGC TTGTCTGCGA GTCCCGAAGC TTTTTCGCTC 
    TGTGAAGTTT CAGTCACACA GGAAGGAGTT ATCAAGCAGA GACGACTGCC TGATCAACTG 
    TCCAAACTGG CTGACAGGAT CCAACTGAGT GCCAGGTACT ATCTAAAGAG CAATATGGAA 
    ACCGAGACGC TGTGTTCGGA TGTGGAAGCA CTGGAGATGC AGAGAGAATC TGTGGTGCCC 
    CTGTTGTCTT TGAGCTCAAT GGAGATAGCC AATCAGCTTT CAGCACGCAA CTATCTGCTC 
    TTCTCCTGCA TCGAACCGAC AGATTACATC AATGACCTCT TTAAGCTTCA CCCACAAGAG 
    CCTCCCACAA ACCTGCGCAA CTTTGAGGGT CTGGTGAACC AGGAGACTTT CTGGGTCGCC 
    ACAGAGATTG TTCAGGAAAC CAACCTGGCC AAAAGAGTGA AGATCATCAA GCACTTTATT 
    AAGATTGCTC TTCACTGCCG CGACTGCAAG AACTTCAACT CTATGTTCGC CATTATCAGT 
    GGGTTCAATC TGGCTCCTGT CTCTCGGTTG CGATCTACAT GGGAACGGTT GCCTGGAAAA 
    TACGAGAAGC TTTTGGCTGA ATTACAGGAT GTTTTTGACC CTTCTCGCAA CATGGCCAAA 
    TATCGCAACC TTTTGAACAA ACACAACCTT CAGCCGCCGG TGATCCCTCT GTTCCCCGTC 
    ATCAAGAAAG ACCTCACCTT CCTTCATGAG GGCAACAACT CAGAGGTGGA CGGTCTGGTG 
    AATTTTGAGA AGTTGAGGAT GATCGCACGA GAGATCAGAC ACGTGGTTCG AATGGCTTCC 
    ATCACCATGG ACCCTGCATT GCTTTTTAAG ACTAGATCTC TAAGTCAGGT GAGCAGCTCT 
    TCTCTGTCTG ACATTGGAAC GATGGGTGGA AAGAGAAAGG GCAGGCGGAG CTCTTTCCTC 
    AGTGCTAAGA AACTGTATGA GGTGGAGATG ATGAGCAGAC GTGTGCGCCA GTATCTGGAC 
    ACGCACTCAA ACGAGTGCAA CGAGGACACA CTTCATGAGC TCTCCATGAC CTGTGAGCCT 
    GCCAACAGCT CCACACTGAA GCACGCTGGT GAGAGGAGAC GGATTGATGC ATCTCCTGCT 
    GGTCAGAGAA GTGCCAGTCC TCAACAAACG AATGGAAATC ATGGTCGCAA GAAGTCAATT 
    AGCAAAGAGT TACCATTCGA GGGCCGAGAC AGACAACGAA AGCTCCCAGA GGACAGCCAA 
    TCAAGCACTT CATCTCTCCT CTCCTCTCCA CGTAATTCAC CTCAGAGCAC ACCTAGAAAA 
    GGCCCACAGT TAGTGGTAAG CGAGGTATCA GATCAGATGA GTCTGTTAAG CTCCTCTTCC 
    TCCGAACTCC TCATGGTTGA TGACCACACA CACACTCAGC CCCATTCACT TGCACATCCA 
    CTCAGTGCAC GCAGGGCTGA GCCAGATCAA TTAAGCCTGG GGTCCTACTC CTTGACTCAG 
    GATCAGTGTG ACCGCGCATC ACTGGACGCA GCAGACAGCG GTCGTGGCAG CTGGACATCC 
    TGCTCGAGTG GATCCCATGA CAATATCCAA AGTATTCCTC AGCGTGTGGG ATATTATGAC 
    AGCCGCAGCT GGGAGATGCT GTCAGACGGG ATGGGGCGAA GTCACATGAC CACTCCCACC 
    GGCTACTGGG CTGATGAACT GGAAGGAGAC ACAGGAACGA TAAAACGCAG AGGTGGAAAA 
    GACGAAACTC CGAACACAAA CAAGAACACC GTGAGTAGGA GGGAGGGGCA TTTCAGAGAG 
    CCGCCGCCCA CACCACCGGG TTACACTGCT GTCTCATTGG CTGAGGCAGA CACCAACAGT 
    CATGCTCATG GGCGACGCCC ACCAGACTAC AGCGCGGCTC TGCAGAGATC TCGATTCCTT 
    AAAAGGACAT GCGATCCTCC TCCATTGCAC TCGTCCAGCA GATTACCACC GTACAGCCAC 
    TGCCAGACTC CACGACGATC ACAAGCAGAT GAAAGCGAAC AAATATCTGC AGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_009291106.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289381.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa46857
    Clone ID Related Accession (Same CDS sequence) XM_009291106.2 , XM_021476310.1 , XM_021474581.1
    Accession Version XM_009291106.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    CTCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289381.1
    CDS608..5029
    Translation

    Target ORF information:

    RefSeq Version XM_009291106.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009291106.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    CTCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021476310.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331985.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa46857
    Clone ID Related Accession (Same CDS sequence) XM_009291106.2 , XM_021476310.1 , XM_021474581.1
    Accession Version XM_021476310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    CTCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331985.1
    CDS589..5010
    Translation

    Target ORF information:

    RefSeq Version XM_021476310.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021476310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    CTCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021474581.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330256.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa46857
    Clone ID Related Accession (Same CDS sequence) XM_009291106.2 , XM_021476310.1 , XM_021474581.1
    Accession Version XM_021474581.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    CTCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330256.1
    CDS589..5010
    Translation

    Target ORF information:

    RefSeq Version XM_021474581.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCACGCGG ACAGAATGGC GTCATATGTG GACAATAGTT TTCGGCACGC GATCATGAAG 
    ATCCCAGCCG ACAGGACGCA ACAGGATCTG CAGATTGTGT ACTCATACCT TCATGGCATG 
    GAAGCTCTGT CCAACCTGAG GGAACACCAG CTCAGGCTGA TGTGTGAGAC GGTTCGATAT 
    GAACAGCATG ATGCTAATGA GGTTTTATAC TATCCTGATG ACATCGGGAC CTGCTGGTAT 
    ATCCTTCTGT CTGGCTCGGT CTTCATAAAG GAGTCTATGT TTCTGCCCCG CAGCAGTTTT 
    GGTAAGCGTT CTGCTGGTAG TTTGCGTCGT GGTTGTGAGT GTATCGTCCT TGAGGCTTCA 
    GAAATGATTG TGGTGGACTA CATGGATGAC AATGACGAGT ATTTCCAAAG ACAAGCATCT 
    CATCGACAAT CCAGGAGAAG GTTTCGAAAG ATAAACCAGC GTGGAGAGCG CCAGACCATT 
    ATTGATACCG TTGATAATTA TCCAGTCAGC AAGCCGCCTC TGCCACCTGG ATACCACTCT 
    GAATGCCCCA AATCCCAGCT CCCAACCGAC TTCTCAAAGC TTCACCCCTC CGACAGTTGC 
    CACTCTCATA TGACTTCCAG CCAATCACGT TCCAGCATCG CCAGTGATTC AGGAAGCAGC 
    AGCCTATCGG ATATTTACCA GGCCACTGAG AGCGAGTGTG GAGACATGGA TCTAAGTGGG 
    CTTCCTGAAA CAGCAGTGGA CTCAGACGAG GATGATGATG ATGAAGACGT AGGAAGGGGA 
    ACGGACCATC TTATGAGTCG GGATATTGTG AGAGACTGCC TTGAAAAAGA CCCTATGGAC 
    CGTACAGATG ATGACATTGA GCAATTGCTG GAGTTTGTTC AGCAGCTTCC AGCATTTGCC 
    AGTCTGAGTG TTTCGGTGCG AAGGGAACTC TGTGCTGTTA TGGTGTTTGC GGTTGTGGAA 
    CGTGCTGGAA CTATAGTCCT AAACCATGGT GAAGAGCTGG ACTCATGGTC AGTCATTCTG 
    AATGGAGCAG TAGAGGTGAT ATACCTGGAC GGCCGCAGTG AGAGTGTGTG TATGGGTGGA 
    AGTTTTGGTG TCTGCCCATC AATGGAGAAA CAACTGATGA CTGGAGTAAT GAGAACCAAA 
    GTTGATGACT GTCAGTTTGT GTGCATTGCC CAGCAGGACT ATTGCTGCAT TCTGAATCAG 
    GTTGAGCAGA ACACACAGCG AGTAGAGGAG GAAGGGGAAA TCGTCATGGT CAAAGAACAT 
    CGAGAACTCG ACAGGACGGG CACACGCAAG GGACACATTG TTGTCAAGGG TACTGCTGAG 
    AGGCTGATGC AGCACTTAGT GGAGGACCAT TCAATCGTCG ACCCCACATA TATTGAGGAC 
    TTTCTCTTAA CCTATCGTAC CTTCCTGCCC AGTCCTCTGA TTCTGGGACA GAGACTTCTA 
    GACTGGTTTA ATGACCCCTG TTTACGGGAC AAGGTAACCC GGGTTGTGCT CTTATGGGTT 
    AACAATCATT TTAGTGATTT TGAAGGAGAC CCTGACATGA CCAGTTTTTT GGAGGAGTTT 
    CAAAGCAACC TGGAAAGAGA GAAAATGACC GGTCAGTTGA GGTTGCTCAA CATCGCTTGT 
    GCAGCAAAAG CCAAACCCAG AGTGATAACT GTAACCAGGA CAGCTCGAGA GATCCCCCTC 
    CCCTTTACCC TTATTGGAGG AGCTGAACGG GGGACACGCC TCTTCATCAG CAGTGTGGAG 
    GTTGGCAGCA AAGCAGAGGA GGCAGGGCTT AAGCGTGGAG ACCAGATATT GGAGGTGAAC 
    GGACAGACGT TTGAGAATGT GCAGTTGTCG AAAGCGACTG AAATTCTCAG GAACAACATA 
    CAGCTCTCTA TGAGTGTCAA AACCAACCTA CTTGTCTTCA AGGAGTTGGT GGCAAGGGTG 
    GCCGAGGAAA GGAAAAACGG CGTTCCTCAT CTTCCAAAGA TTGGTGACGG AAAGAAAAAC 
    TGTCGATATT CTATACCTGA ACTTGGGGCT GGTTCTGATG TTATAGGTCA AGAAAAAATC 
    AGCAAGAAGG CAAAAGCACA TACAGTGGGT GGCCGAAACA AACTGATGAA GATTCTAGAC 
    AAGACACGCA TGAGCATCCT ACCTCAGAAA CCTTACAGTG ACCTCGGGTT GGGGCAGACT 
    CAGGATGACA GCATTGTGGG ATTACGGCAG AACAAACAGA CTCCACCCAC TATGCCAGTG 
    AGCGGAACTC TCTCATCCAG TAACCCTGAC CTGGCACAAG GCCAGCAACG CATCATCGAC 
    TACAGTACAC AGCCACCAGA GTTGCAAGAC CAGGTGCTGC GCATTTTCAA GGCAGATCAG 
    CAAAGCCGTT ACTTACTGGC CAATAGAGAC ACCACAGCCA GAGAAGCTGC CAATCTAGCC 
    ATAAAAGAGT TTGGCTTGTC TGCGAGTCCC GAAGCTTTTT CGCTCTGTGA AGTTTCAGTC 
    ACACAGGAAG GAGTTATCAA GCAGAGACGA CTGCCTGATC AACTGTCCAA ACTGGCTGAC 
    AGGATCCAAC TGAGTGCCAG GTACTATCTA AAGAGCAATA TGGAAACCGA GACGCTGTGT 
    TCGGATGTGG AAGCACTGGA GATGCAGAGA GAATCTGTGG TGCCCCTGTT GTCTTTGAGC 
    TCAATGGAGA TAGCCAATCA GCTTTCAGCA CGCAACTATC TGCTCTTCTC CTGCATCGAA 
    CCGACAGATT ACATCAATGA CCTCTTTAAG CTTCACCCAC AAGAGCCTCC CACAAACCTG 
    CGCAACTTTG AGGGTCTGGT GAACCAGGAG ACTTTCTGGG TCGCCACAGA GATTGTTCAG 
    GAAACCAACC TGGCCAAAAG AGTGAAGATC ATCAAGCACT TTATTAAGAT TGCTCTTCAC 
    TGCCGCGACT GCAAGAACTT CAACTCTATG TTCGCCATTA TCAGTGGGTT CAATCTGGCT 
    CCTGTCTCTC GGTTGCGATC TACATGGGAA CGGTTGCCTG GAAAATACGA GAAGCTTTTG 
    GCTGAATTAC AGGATGTTTT TGACCCTTCT CGCAACATGG CCAAATATCG CAACCTTTTG 
    AACAAACACA ACCTTCAGCC GCCGGTGATC CCTCTGTTCC CCGTCATCAA GAAAGACCTC 
    ACCTTCCTTC ATGAGGGCAA CAACTCAGAG GTGGACGGTC TGGTGAATTT TGAGAAGTTG 
    AGGATGATCG CACGAGAGAT CAGACACGTG GTTCGAATGG CTTCCATCAC CATGGACCCT 
    GCATTGCTTT TTAAGACTAG ATCTCTAAGT CAGGTGAGCA GCTCTTCTCT GTCTGACATT 
    GGAACGATGG GTGGAAAGAG AAAGGGCAGG CGGAGCTCTT TCCTCAGTGC TAAGAAACTG 
    TATGAGGTGG AGATGATGAG CAGACGTGTG CGCCAGTATC TGGACACGCA CTCAAACGAG 
    TGCAACGAGG ACACACTTCA TGAGCTCTCC ATGACCTGTG AGCCTGCCAA CAGCTCCACA 
    CTGAAGCACG CTGGTGAGAG GAGACGGATT GATGCATCTC CTGCTGGTCA GAGAAGTGCC 
    AGTCCTCAAC AAACGAATGG AAATCATGGT CGCAAGAAGT CAATTAGCAA AGAGTTACCA 
    TTCGAGGGCC GAGACAGACA ACGAAAGCTC CCAGAGGACA GCCAATCAAG CACTTCATCT 
    CTCCTCTCCT CTCCACGTAA TTCACCTCAG AGCACACCTA GAAAAGGCCC ACAGTTAGTG 
    GTAAGCGAGG TATCAGATCA GATGAGTCTG TTAAGCTCCT CTTCCTCCGA ACTCCTCATG 
    GTTGATGACC ACACACACAC TCAGCCCCAT TCACTTGCAC ATCCACTCAG TGCACGCAGG 
    GCTGAGCCAG ATCAATTAAG CCTGGGGTCC TACTCCTTGA CTCAGGATCA GTGTGACCGC 
    GCATCACTGG ACGCAGCAGA CAGCGGTCGT GGCAGCTGGA CATCCTGCTC GAGTGGATCC 
    CATGACAATA TCCAAAGTAT TCCTCAGCGT GTGGGATATT ATGACAGCCG CAGCTGGGAG 
    ATGCTGTCAG ACGGGATGGG GCGAAGTCAC ATGACCACTC CCACCGGCTA CTGGGCTGAT 
    GAACTGGAAG GAGACACAGG AACGATAAAA CGCAGAGGTG GAAAAGACGA AACTCCGAAC 
    ACAAACAAGA ACACCGTGAG TAGGAGGGAG GGGCATTTCA GAGAGCCGCC GCCCACACCA 
    CCGGGTTACA CTGCTGTCTC ATTGGCTGAG GCAGACACCA ACAGTCATGC TCATGGGCGA 
    CGCCCACCAG ACTACAGCGC GGCTCTGCAG AGATCTCGAT TCCTTAAAAG GACATGCGAT 
    CCTCCTCCAT TGCACTCGTC CAGCAGATTA CCACCGTACA GCCACTGCCA GACTCCACGA 
    CGATCACAAG CAGATGAAAG CGAACAAATA TCTGCAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021474582.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330257.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa46858
    Clone ID Related Accession (Same CDS sequence) XM_021474582.1 , XM_021476313.1 , XM_009291107.1
    Accession Version XM_021474582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTCT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330257.1
    CDS94..4173
    Translation

    Target ORF information:

    RefSeq Version XM_021474582.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021474582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTCT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_021476313.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331988.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa46858
    Clone ID Related Accession (Same CDS sequence) XM_021474582.1 , XM_021476313.1 , XM_009291107.1
    Accession Version XM_021476313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTCT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331988.1
    CDS94..4173
    Translation

    Target ORF information:

    RefSeq Version XM_021476313.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021476313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTCT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    si:dkey-253i9.4 ( XM_009291107.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289382.1 Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa46858
    Clone ID Related Accession (Same CDS sequence) XM_021474582.1 , XM_021476313.1 , XM_009291107.1
    Accession Version XM_009291107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rap guanine nucleotide exchange factor 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTCT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289382.1
    CDS94..4173
    Translation

    Target ORF information:

    RefSeq Version XM_009291107.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-253i9.4 (si:dkey-253i9.4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009291107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATGACA ATGACGAGTA TTTCCAAAGA CAAGCATCTC ATCGACAATC CAGGAGAAGG 
    TTTCGAAAGA TAAACCAGCG TGGAGAGCGC CAGACCATTA TTGATACCGT TGATAATTAT 
    CCAGTCAGCA AGCCGCCTCT GCCACCTGGA TACCACTCTG AATGCCCCAA ATCCCAGCTC 
    CCAACCGACT TCTCAAAGCT TCACCCCTCC GACAGTTGCC ACTCTCATAT GACTTCCAGC 
    CAATCACGTT CCAGCATCGC CAGTGATTCA GGAAGCAGCA GCCTATCGGA TATTTACCAG 
    GCCACTGAGA GCGAGTGTGG AGACATGGAT CTAAGTGGGC TTCCTGAAAC AGCAGTGGAC 
    TCAGACGAGG ATGATGATGA TGAAGACGTA GGAAGGGGAA CGGACCATCT TATGAGTCGG 
    GATATTGTGA GAGACTGCCT TGAAAAAGAC CCTATGGACC GTACAGATGA TGACATTGAG 
    CAATTGCTGG AGTTTGTTCA GCAGCTTCCA GCATTTGCCA GTCTGAGTGT TTCGGTGCGA 
    AGGGAACTCT GTGCTGTTAT GGTGTTTGCG GTTGTGGAAC GTGCTGGAAC TATAGTCCTA 
    AACCATGGTG AAGAGCTGGA CTCATGGTCA GTCATTCTGA ATGGAGCAGT AGAGGTGATA 
    TACCTGGACG GCCGCAGTGA GAGTGTGTGT ATGGGTGGAA GTTTTGGTGT CTGCCCATCA 
    ATGGAGAAAC AACTGATGAC TGGAGTAATG AGAACCAAAG TTGATGACTG TCAGTTTGTG 
    TGCATTGCCC AGCAGGACTA TTGCTGCATT CTGAATCAGG TTGAGCAGAA CACACAGCGA 
    GTAGAGGAGG AAGGGGAAAT CGTCATGGTC AAAGAACATC GAGAACTCGA CAGGACGGGC 
    ACACGCAAGG GACACATTGT TGTCAAGGGT ACTGCTGAGA GGCTGATGCA GCACTTAGTG 
    GAGGACCATT CAATCGTCGA CCCCACATAT ATTGAGGACT TTCTCTTAAC CTATCGTACC 
    TTCCTGCCCA GTCCTCTGAT TCTGGGACAG AGACTTCTAG ACTGGTTTAA TGACCCCTGT 
    TTACGGGACA AGGTAACCCG GGTTGTGCTC TTATGGGTTA ACAATCATTT TAGTGATTTT 
    GAAGGAGACC CTGACATGAC CAGTTTTTTG GAGGAGTTTC AAAGCAACCT GGAAAGAGAG 
    AAAATGACCG GTCAGTTGAG GTTGCTCAAC ATCGCTTGTG CAGCAAAAGC CAAACCCAGA 
    GTGATAACTG TAACCAGGAC AGCTCGAGAG ATCCCCCTCC CCTTTACCCT TATTGGAGGA 
    GCTGAACGGG GGACACGCCT CTTCATCAGC AGTGTGGAGG TTGGCAGCAA AGCAGAGGAG 
    GCAGGGCTTA AGCGTGGAGA CCAGATATTG GAGGTGAACG GACAGACGTT TGAGAATGTG 
    CAGTTGTCGA AAGCGACTGA AATTCTCAGG AACAACATAC AGCTCTCTAT GAGTGTCAAA 
    ACCAACCTAC TTGTCTTCAA GGAGTTGGTG GCAAGGGTGG CCGAGGAAAG GAAAAACGGC 
    GTTCCTCATC TTCCAAAGAT TGGTGACGGA AAGAAAAACT GTCGATATTC TATACCTGAA 
    CTTGGGGCTG GTTCTGATGT TATAGGTCAA GAAAAAATCA GCAAGAAGGC AAAAGCACAT 
    ACAGTGGGTG GCCGAAACAA ACTGATGAAG ATTCTAGACA AGACACGCAT GAGCATCCTA 
    CCTCAGAAAC CTTACAGTGA CCTCGGGTTG GGGCAGACTC AGGATGACAG CATTGTGGGA 
    TTACGGCAGA ACAAACAGAC TCCACCCACT ATGCCAGTGA GCGGAACTCT CTCATCCAGT 
    AACCCTGACC TGGCACAAGG CCAGCAACGC ATCATCGACT ACAGTACACA GCCACCAGCA 
    CCTGGATTCA CTCAGTTGCA AGACCAGGTG CTGCGCATTT TCAAGGCAGA TCAGCAAAGC 
    CGTTACTTAC TGGCCAATAG AGACACCACA GCCAGAGAAG CTGCCAATCT AGCCATAAAA 
    GAGTTTGGCT TGTCTGCGAG TCCCGAAGCT TTTTCGCTCT GTGAAGTTTC AGTCACACAG 
    GAAGGAGTTA TCAAGCAGAG ACGACTGCCT GATCAACTGT CCAAACTGGC TGACAGGATC 
    CAACTGAGTG CCAGGTACTA TCTAAAGAGC AATATGGAAA CCGAGACGCT GTGTTCGGAT 
    GTGGAAGCAC TGGAGATGCA GAGAGAATCT GTGGTGCCCC TGTTGTCTTT GAGCTCAATG 
    GAGATAGCCA ATCAGCTTTC AGCACGCAAC TATCTGCTCT TCTCCTGCAT CGAACCGACA 
    GATTACATCA ATGACCTCTT TAAGCTTCAC CCACAAGAGC CTCCCACAAA CCTGCGCAAC 
    TTTGAGGGTC TGGTGAACCA GGAGACTTTC TGGGTCGCCA CAGAGATTGT TCAGGAAACC 
    AACCTGGCCA AAAGAGTGAA GATCATCAAG CACTTTATTA AGATTGCTCT TCACTGCCGC 
    GACTGCAAGA ACTTCAACTC TATGTTCGCC ATTATCAGTG GGTTCAATCT GGCTCCTGTC 
    TCTCGGTTGC GATCTACATG GGAACGGTTG CCTGGAAAAT ACGAGAAGCT TTTGGCTGAA 
    TTACAGGATG TTTTTGACCC TTCTCGCAAC ATGGCCAAAT ATCGCAACCT TTTGAACAAA 
    CACAACCTTC AGCCGCCGGT GATCCCTCTG TTCCCCGTCA TCAAGAAAGA CCTCACCTTC 
    CTTCATGAGG GCAACAACTC AGAGGTGGAC GGTCTGGTGA ATTTTGAGAA GTTGAGGATG 
    ATCGCACGAG AGATCAGACA CGTGGTTCGA ATGGCTTCCA TCACCATGGA CCCTGCATTG 
    CTTTTTAAGA CTAGGAAGAA AAAGTGGAGG AGTCTTGGAT CTCTAAGTCA GGTGAGCAGC 
    TCTTCTCTGT CTGACATTGG AACGATGGGT GGAAAGAGAA AGGGCAGGCG GAGCTCTTTC 
    CTCAGTGCTA AGAAACTGTA TGAGGTGGAG ATGATGAGCA GACGTGTGCG CCAGTATCTG 
    GACACGCACT CAAACGAGTG CAACGAGGAC ACACTTCATG AGCTCTCCAT GACCTGTGAG 
    CCTGCCAACA GCTCCACACT GAAGCACGCT GGTGAGAGGA GACGGATTGA TGCATCTCCT 
    GCTGGTCAGA GAAGTGCCAG TCCTCAACAA ACGAATGGAA ATCATGGTCG CAAGAAGTCA 
    ATTAGCAAAG AGTTACCATT CGAGGGCCGA GACAGACAAC GAAAGCTCCC AGAGGACAGC 
    CAATCAAGCA CTTCATCTCT CCTCTCCTCT CCACGTAATT CACCTCAGAG CACACCTAGA 
    AAAGGCCCAC AGTTAGTGGT AAGCGAGGTA TCAGATCAGA TGAGTCTGTT AAGCTCCTCT 
    TCCTCCGAAC TCCTCATGGT TGATGACCAC ACACACACTC AGCCCCATTC ACTTGCACAT 
    CCACTCAGTG CACGCAGGGC TGAGCCAGAT CAATTAAGCC TGGGGTCCTA CTCCTTGACT 
    CAGGATCAGT GTGACCGCGC ATCACTGGAC GCAGCAGACA GCGGTCGTGG CAGCTGGACA 
    TCCTGCTCGA GTGGATCCCA TGACAATATC CAAAGTATTC CTCAGCGTGT GGGATATTAT 
    GACAGCCGCA GCTGGGAGAT GCTGTCAGAC GGGATGGGGC GAAGTCACAT GACCACTCCC 
    ACCGGCTACT GGGCTGATGA ACTGGAAGGA GACACAGGAA CGATAAAACG CAGAGGTGGA 
    AAAGACGAAA CTCCGAACAC AAACAAGAAC ACCGTGAGTA GGAGGGAGGG GCATTTCAGA 
    GAGCCGCCGC CCACACCACC GGGTTACACT GCTGTCTCAT TGGCTGAGGC AGACACCAAC 
    AGTCATGCTC ATGGGCGACG CCCACCAGAC TACAGCGCGG CTCTGCAGAG ATCTCGATTC 
    CTTAAAAGGA CATGCGATCC TCCTCCATTG CACTCGTCCA GCAGATTACC ACCGTACAGC 
    CACTGCCAGA CTCCACGACG ATCACAAGCA GATGAAAGCG AACAAATATC TGCAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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