ECE2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECE2B
Entrez Gene ID	793465
Full Name	endothelin converting enzyme 2b
Synonyms	ECE2,si:dkey-83h2.6
General protein information	Preferred Names endothelin converting enzyme 2b Names endothelin-converting enzyme 2 endothelin converting enzyme 2 karneol
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351043.1	XP_017206532.1	endothelin-converting enzyme 2 isoform X2
XM_017351044.1	XP_017206533.1	endothelin-converting enzyme 2 isoform X3
XM_017351042.2	XP_017206531.1	endothelin-converting enzyme 2 isoform X1
XM_017351043.2	XP_017206532.1	endothelin-converting enzyme 2 isoform X2
XM_017351044.2	XP_017206533.1	endothelin-converting enzyme 2 isoform X3
NM_001302239.1	NP_001289168.1	endothelin-converting enzyme 2
XM_017351042.1	XP_017206531.1	endothelin-converting enzyme 2 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The construction of intrahepatic cholangiocarcinoma model in zebrafish.
Wang J, Leng X, Wang G, Wan X, Cao H
Scientific reports7(1)13419(2017 Oct)

Microenvironment-derived factors driving metastatic plasticity in melanoma.
Kim IS, Heilmann S, Kansler ER, Zhang Y, Zimmer M, Ratnakumar K, Bowman RL, Simon-Vermot T, Fennell M, Garippa R, Lu L, Lee W, Hollmann T, Xavier JB, White RM
Nature communications814343(2017 Feb)

Endothelin signalling in iridophore development and stripe pattern formation of zebrafish.
Krauss J, Frohnh?fer HG, Walderich B, Maischein HM, Weiler C, Irion U, N?sslein-Volhard C
Biology open3(6)503-9(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ECE2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46818	NM_001302239.1 Latest version!	Danio rerio endothelin converting enzyme 2b (ece2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa55148	XM_017351042.2 Latest version!	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa55149	XM_017351043.2 Latest version!	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa55150	XM_017351044.2 Latest version!	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa55149	XM_017351043.1	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa55150	XM_017351044.1	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa55148	XM_017351042.1	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECE2B ( NM_001302239.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001289168.1 Danio rerio endothelin converting enzyme 2b (ece2b), mRNA.

Price & Availability↑

CloneID	ODa46818
Clone ID Related Accession (Same CDS sequence)	NM_001302239.1
Accession Version	NM_001302239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-04
Organism	Danio rerio(zebrafish)
Product	endothelin-converting enzyme 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KJ622365.1. ##Evidence-Data-START## Transcript exon combination :: KJ622365.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGTAG CGCTGCAAGA TTTACGAAAT AACATGTCTA ACTACAAGCG TGCAACGTTT 
GAGGAGGAGG ACGGGACCGA TGTCCCTGTT GATGGAGCCA TTTCTCCTGA CAGTGTTGAG 
GTGGGTTTCC GTAAGGGTGG TATTCAGTTG TTTGGTCCAC TGGGCCGGAG GACTCAGCTG 
GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT TCACTTCTTG CACTGTTCGG CTGTGCGGTC 
ACACTGGGAG TCCGTTATAA CAGAGATCCA GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT 
GTCACTGTGG CCAGCAAGAT TGTTGAAGCT TTGGACCGCT CTGCCGACCC ATGTCAGGAT 
TTTTACCAAT ACGCCTGTGG AGGATGGGTC CGCAAAAACC CCCTGCCAGA TGGCCGGTCC 
CGCTGGAGCA CATTTAACAG CATCTGGGAC CAGAACCAGG CTGTGCTCAA ACACCTGCTG 
GAAAACGGCA CTTTTAACTC CAGCAGTGAA GCAGAGAGGA AGACACAGTC ATACTACCTT 
TCCTGTCTTA ATGAACAGCG AATAGAAGAG CTCGGAGCCC AACCACTCAT GGATTTGATC 
ACAAAGATCG GAGGCTGGAA CATCACAGAG TCCTGGGACA AAGAGAATTT TCTGGATGTT 
TTAAAGATCA TTTCTGGACC ATACAGAGCT CAGCCTTTCT TCACCGTCAG TGTTAGCGTA 
GACCCCAAAA ACTCCAACAG CAATGTCATT CAGGTCGACC AATCAGGACT CTTCCTCCCG 
TCCCGAGATT ATTACCTCAA TAAGACTAAC GAGAAGGTTT TGAAAGCTTA TCTGGATTAC 
ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA GACAAGAATT CCACTCGGGG TCAGATGCAG 
CAGATTTTGG ACTTTGAGAC GGCCCTGGCG AACATCACCG TGCCTCAGGA CGAACGGCGA 
GACGAGGAGA AGATCTACCA CAAGATAACC ATAGCTGACC TCCAGGTCTT GGCTCCGGCT 
ATTGAATGGT TGGATTATCT GAACTCAGTA CTTTCACCGC TGGAGCTGAA CGACACAGAA 
CCGGTGGTGG TGTATGCTAA AGAGTACATG CAACAAGTTT CAGAGCTCAT CAACAAGACC 
GACCACAGTC TTCTGAATAA TTACATGATC TGGAATCTGG TGCAGAAAGG AGCGTCCAGC 
CTTGATCAGC GCTTTGAAAA TGCTCAGGAT AAACTGCTGG AGAGCCTCTA TGGCACCAAA 
AAGTCTTGTA CTCCGCGCTG GCAGACGTGC ATTGGAAACA CAGACGACAC CCTCGGTTTT 
GCACTGGGAG CTCTTTTCGT CAAAGCCACC TTCGACAAAC AGAGCAAAGA AATAGCAGAG 
GGGATGATCA ATGAAATCCG CACGGCTTTC AAAGGCGCGC TGGATGATTT GAAGTGGATG 
GATGAACAGA CCCGACAAGC TGCCAAGGAC AAGGCGGATG CCATCTATGA CATGATCGGA 
TTTCCAGACT TCATTCTGGA CTCCAAAGAG CTGGATGATG TGTATGATGG GTATGAGGTT 
ACGGAGGACA ACTTCTTCCA GAACATGATC AACTTCTATA ACTTCTCAGC ACGAGTGATG 
GCAGATCAGC TCCGCAAGCC GCCAAACAGA GACCAATGGA GTATGACTCC ACCGACAGTG 
AACGCTTACT ACATGCCAAC CAAGAACGGA ATCGTCTTTC CCGCTGGCAT CTTACAGGCC 
CCGTTCTACG CCCAGGATCA CCCCAAAGCT TTAAACTTTG GTGGGATTGG AGTGGTCATG 
GGACATGAGC TAACGCATGC GTTTGATGAC CAAGGCCGAG AGTATGACAA AGATGGAAAC 
CTGCGCTCGT GGTGGCAGAA CTCCTCGGTG GAGGCCTTCA AGAACCGCAC CGAGTGCATG 
GTCGATCAAT ACACACAGTA CACCATCAAC GGAGAGCACA TTAATGGAAA ACAGACGCTG 
GGAGAAAACA TCGCAGACAA TGGCGGCCTG AAAGCTGCCT ATCATGCGTA CAGGTCCTGG 
GTGCAGAAGA ATGGGGAAGA AAAGAGACTC CCAGCTGTCA ATCTGACCAA CGACCAGCTC 
TTCTTTGTGG GTTTCGCACA GGTGTGGTGC TCTGTCCGGA CGCCAGAAAG CGCTCACGAG 
GGCCTGATGA CGGATCCTCA CAGTCCGCCC AAATACCGCG TGATCGGCAC CCTGTCCAAC 
TCACCCGAAT TCGCCGAGCA CTTCCAATGT CCGCTGGGCT CCTCCATGAA CTCAGGCCAC 
CGCTGCGAGG TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289168.1
CDS	175..2472
Translation	MSVALQDLRNNMSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	NM_001302239.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302239.1

ORF Insert Sequence:

ATGAGTGTAG CGCTGCAAGA TTTACGAAAT AACATGTCTA ACTACAAGCG TGCAACGTTT 
GAGGAGGAGG ACGGGACCGA TGTCCCTGTT GATGGAGCCA TTTCTCCTGA CAGTGTTGAG 
GTGGGTTTCC GTAAGGGTGG TATTCAGTTG TTTGGTCCAC TGGGCCGGAG GACTCAGCTG 
GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT TCACTTCTTG CACTGTTCGG CTGTGCGGTC 
ACACTGGGAG TCCGTTATAA CAGAGATCCA GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT 
GTCACTGTGG CCAGCAAGAT TGTTGAAGCT TTGGACCGCT CTGCCGACCC ATGTCAGGAT 
TTTTACCAAT ACGCCTGTGG AGGATGGGTC CGCAAAAACC CCCTGCCAGA TGGCCGGTCC 
CGCTGGAGCA CATTTAACAG CATCTGGGAC CAGAACCAGG CTGTGCTCAA ACACCTGCTG 
GAAAACGGCA CTTTTAACTC CAGCAGTGAA GCAGAGAGGA AGACACAGTC ATACTACCTT 
TCCTGTCTTA ATGAACAGCG AATAGAAGAG CTCGGAGCCC AACCACTCAT GGATTTGATC 
ACAAAGATCG GAGGCTGGAA CATCACAGAG TCCTGGGACA AAGAGAATTT TCTGGATGTT 
TTAAAGATCA TTTCTGGACC ATACAGAGCT CAGCCTTTCT TCACCGTCAG TGTTAGCGTA 
GACCCCAAAA ACTCCAACAG CAATGTCATT CAGGTCGACC AATCAGGACT CTTCCTCCCG 
TCCCGAGATT ATTACCTCAA TAAGACTAAC GAGAAGGTTT TGAAAGCTTA TCTGGATTAC 
ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA GACAAGAATT CCACTCGGGG TCAGATGCAG 
CAGATTTTGG ACTTTGAGAC GGCCCTGGCG AACATCACCG TGCCTCAGGA CGAACGGCGA 
GACGAGGAGA AGATCTACCA CAAGATAACC ATAGCTGACC TCCAGGTCTT GGCTCCGGCT 
ATTGAATGGT TGGATTATCT GAACTCAGTA CTTTCACCGC TGGAGCTGAA CGACACAGAA 
CCGGTGGTGG TGTATGCTAA AGAGTACATG CAACAAGTTT CAGAGCTCAT CAACAAGACC 
GACCACAGTC TTCTGAATAA TTACATGATC TGGAATCTGG TGCAGAAAGG AGCGTCCAGC 
CTTGATCAGC GCTTTGAAAA TGCTCAGGAT AAACTGCTGG AGAGCCTCTA TGGCACCAAA 
AAGTCTTGTA CTCCGCGCTG GCAGACGTGC ATTGGAAACA CAGACGACAC CCTCGGTTTT 
GCACTGGGAG CTCTTTTCGT CAAAGCCACC TTCGACAAAC AGAGCAAAGA AATAGCAGAG 
GGGATGATCA ATGAAATCCG CACGGCTTTC AAAGGCGCGC TGGATGATTT GAAGTGGATG 
GATGAACAGA CCCGACAAGC TGCCAAGGAC AAGGCGGATG CCATCTATGA CATGATCGGA 
TTTCCAGACT TCATTCTGGA CTCCAAAGAG CTGGATGATG TGTATGATGG GTATGAGGTT 
ACGGAGGACA ACTTCTTCCA GAACATGATC AACTTCTATA ACTTCTCAGC ACGAGTGATG 
GCAGATCAGC TCCGCAAGCC GCCAAACAGA GACCAATGGA GTATGACTCC ACCGACAGTG 
AACGCTTACT ACATGCCAAC CAAGAACGGA ATCGTCTTTC CCGCTGGCAT CTTACAGGCC 
CCGTTCTACG CCCAGGATCA CCCCAAAGCT TTAAACTTTG GTGGGATTGG AGTGGTCATG 
GGACATGAGC TAACGCATGC GTTTGATGAC CAAGGCCGAG AGTATGACAA AGATGGAAAC 
CTGCGCTCGT GGTGGCAGAA CTCCTCGGTG GAGGCCTTCA AGAACCGCAC CGAGTGCATG 
GTCGATCAAT ACACACAGTA CACCATCAAC GGAGAGCACA TTAATGGAAA ACAGACGCTG 
GGAGAAAACA TCGCAGACAA TGGCGGCCTG AAAGCTGCCT ATCATGCGTA CAGGTCCTGG 
GTGCAGAAGA ATGGGGAAGA AAAGAGACTC CCAGCTGTCA ATCTGACCAA CGACCAGCTC 
TTCTTTGTGG GTTTCGCACA GGTGTGGTGC TCTGTCCGGA CGCCAGAAAG CGCTCACGAG 
GGCCTGATGA CGGATCCTCA CAGTCCGCCC AAATACCGCG TGATCGGCAC CCTGTCCAAC 
TCACCCGAAT TCGCCGAGCA CTTCCAATGT CCGCTGGGCT CCTCCATGAA CTCAGGCCAC 
CGCTGCGAGG TTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2B ( XM_017351042.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206531.1 Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55148
Clone ID Related Accession (Same CDS sequence)	XM_017351042.2 , XM_017351042.1
Accession Version	XM_017351042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endothelin-converting enzyme 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTAG CGCTGCAAGA TTTACGAAAT AACATGTCTA ACTACAAGCG TGCAACGTTT 
GAGGAGGAGG ACGGGACCGA TGTCCCTGTT GATGGAGCCA TTTCTCCTGA CAGTGTTGAG 
GTGGGTTTCC GTAAGGGTGG TATTCAGTTG TTTGGTCCAC TGGGCCGGAG GACTCAGCTG 
GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT TCACTTCTTG CACTGTTCGG CTGTGCGGTC 
ACACTGGGAG TCCGTTATAA CAGAGATCCA GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT 
GTCACTGTGG CCAGCAAGAT TGTTGAAGCT TTGGACCGCT CTGCCGACCC ATGTCAGGAT 
TTTTACCAAT ACGCCTGTGG AGGATGGGTC CGCAAAAACC CCCTGCCAGA TGGCCGGTCC 
CGCTGGAGCA CATTTAACAG CATCTGGGAC CAGAACCAGG CTGTGCTCAA ACACCTGCTG 
GAAAATGGCA CTTTTAACTC CAGCAGTGAA GCAGAGAGGA AGACACAGTC GTACTACCTT 
TCCTGTCTTA ATGAACAGCG AATAGAAGAA CTCGGAGCCC AACCACTCAT GGATTTGATT 
ACAAAGATCG GAGGCTGGAA CATCACAGAG TCCTGGGACA AAGAGAATTT TCTGGATGTT 
TTAAAGATCA TTTCTGGACC ATACAGAGCT CAGCCCTTCT TCACCGTCAG TGTTAGCGTA 
GACCCCAAAA ACTCCAACAG CAATGTGATT CAGGTCGACC AATCAGGACT CTTCCTCCCG 
TCCCGAGATT ATTACCTCAA TAAGACTAAC GAGAAGGTTT TGAAAGCTTA TCTGGATTAC 
ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA GACAAGAATT CCACTCGGGG TCAGATGCAG 
CAGATTTTGG ACTTTGAGAC GGCGCTGGCG AACATCACCG TGCCCCAGGA CGAACGGCGA 
GACGAGGAGA AGATCTACCA CAAGATAACC ATAGCTGACC TCCAGGTCTT GGCTCCGGCT 
ATTGAATGGT TGGATTATCT GAACTCAGTA CTTTCACCGC TGGAGCTGAA CGACACAGAA 
CCGGTGGTGG TGTATGCTAA AGAGTACATG CAACAAGTTT CAGAGCTCAT CAACAAGACC 
GACCACAGTC TTCTGAATAA TTACATGATC TGGAATCTGG TGCAGAAAGG AGCGTCCAGC 
CTTGATCAGC GCTTTGAAAA TGCTCAGGAT AAACTGCTGG AGAGCCTCTA TGGCACCAAA 
AAGTCTTGTA CTCCGCGCTG GCAGACGTGC ATTGGAAACA CAGACGACAC CCTCGGTTTT 
GCACTGGGAG CTCTTTTCGT CAAAGCCACC TTCGACAAAC AGAGCAAAGA AATAGACCTC 
AACGGTGGTG CGTTTTTGCC CTCATGTGGC CGTAATGTGA ACTCCACAGT TCACAACCTC 
ATGTTTTCTG TCATAACGCA GGCAGAGGGG ATGATCAATG AAATCCGCAC GGCTTTCAAA 
GGCGCGCTGG ATGATTTGAA GTGGATGGAT GAACAGACCC GACAAGCTGC CAAGGACAAG 
GCGGATGCCA TCTATGACAT GATCGGATTT CCAGACTTCA TTCTGGACTC CAAAGAGCTG 
GATGATGTGT ATGATGGGTA TGAGGTTACG GAGGACAACT TCTTCCAGAA CATGATTAAC 
TTCTATAACT TCTCAGCACG AGTGATGGCA GATCAGCTCC GCAAGCCACC AAACAGAGAC 
CAATGGAGTA TGACTCCACC GACAGTGAAC GCTTACTACA TGCCAACCAA GAACGGAATC 
GTCTTTCCCG CTGGCATCTT ACAGGCCCCG TTCTACGCCC AGGATCACCC CAAAGCTTTA 
AACTTTGGTG GGATTGGAGT GGTCATGGGA CATGAGCTAA CGCATGCGTT TGATGACCAA 
GGCCGAGAGT ACGACAAAGA TGGAAACCTG CGCTCGTGGT GGCAGAACTC CTCGGTGGAG 
GCCTTCAAGA ACCGCACCGA GTGCATGGTC GATCAATACA CACAGTACAC CATCAACGGA 
GAGCACATTA ATGGAAAACA GACGCTGGGA GAAAACATCG CAGACAATGG CGGCCTGAAA 
GCTGCCTATC ATGCGTACAG GTCCTGGGTG CAGAAGAATG GGGAAGAGAA GAGACTCCCA 
GCTGTCAATC TGACAAACGA CCAGCTCTTC TTTGTGGGTT TTGCACAGGT GTGGTGCTCT 
GTCCGGACGC CAGAAAGCGC TCACGAGGGC CTGATGACGG ATCCTCACAG TCCGCCCAAA 
TACCGCGTGA TCGGCACCCT GTCTAACTCA CCCGAGTTCG CCGAGCACTT CCAATGTCCG 
CTGGGCTCCT CCATGAACTC AGGCCACCGC TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206531.1
CDS	425..2809
Translation	MSVALQDLRNNMSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIDLNGGAFLPSCGRNVNSTVHNLMFSVITQAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	XM_017351042.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351042.2

ORF Insert Sequence:

ATGAGTGTAG CGCTGCAAGA TTTACGAAAT AACATGTCTA ACTACAAGCG TGCAACGTTT 
GAGGAGGAGG ACGGGACCGA TGTCCCTGTT GATGGAGCCA TTTCTCCTGA CAGTGTTGAG 
GTGGGTTTCC GTAAGGGTGG TATTCAGTTG TTTGGTCCAC TGGGCCGGAG GACTCAGCTG 
GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT TCACTTCTTG CACTGTTCGG CTGTGCGGTC 
ACACTGGGAG TCCGTTATAA CAGAGATCCA GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT 
GTCACTGTGG CCAGCAAGAT TGTTGAAGCT TTGGACCGCT CTGCCGACCC ATGTCAGGAT 
TTTTACCAAT ACGCCTGTGG AGGATGGGTC CGCAAAAACC CCCTGCCAGA TGGCCGGTCC 
CGCTGGAGCA CATTTAACAG CATCTGGGAC CAGAACCAGG CTGTGCTCAA ACACCTGCTG 
GAAAATGGCA CTTTTAACTC CAGCAGTGAA GCAGAGAGGA AGACACAGTC GTACTACCTT 
TCCTGTCTTA ATGAACAGCG AATAGAAGAA CTCGGAGCCC AACCACTCAT GGATTTGATT 
ACAAAGATCG GAGGCTGGAA CATCACAGAG TCCTGGGACA AAGAGAATTT TCTGGATGTT 
TTAAAGATCA TTTCTGGACC ATACAGAGCT CAGCCCTTCT TCACCGTCAG TGTTAGCGTA 
GACCCCAAAA ACTCCAACAG CAATGTGATT CAGGTCGACC AATCAGGACT CTTCCTCCCG 
TCCCGAGATT ATTACCTCAA TAAGACTAAC GAGAAGGTTT TGAAAGCTTA TCTGGATTAC 
ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA GACAAGAATT CCACTCGGGG TCAGATGCAG 
CAGATTTTGG ACTTTGAGAC GGCGCTGGCG AACATCACCG TGCCCCAGGA CGAACGGCGA 
GACGAGGAGA AGATCTACCA CAAGATAACC ATAGCTGACC TCCAGGTCTT GGCTCCGGCT 
ATTGAATGGT TGGATTATCT GAACTCAGTA CTTTCACCGC TGGAGCTGAA CGACACAGAA 
CCGGTGGTGG TGTATGCTAA AGAGTACATG CAACAAGTTT CAGAGCTCAT CAACAAGACC 
GACCACAGTC TTCTGAATAA TTACATGATC TGGAATCTGG TGCAGAAAGG AGCGTCCAGC 
CTTGATCAGC GCTTTGAAAA TGCTCAGGAT AAACTGCTGG AGAGCCTCTA TGGCACCAAA 
AAGTCTTGTA CTCCGCGCTG GCAGACGTGC ATTGGAAACA CAGACGACAC CCTCGGTTTT 
GCACTGGGAG CTCTTTTCGT CAAAGCCACC TTCGACAAAC AGAGCAAAGA AATAGACCTC 
AACGGTGGTG CGTTTTTGCC CTCATGTGGC CGTAATGTGA ACTCCACAGT TCACAACCTC 
ATGTTTTCTG TCATAACGCA GGCAGAGGGG ATGATCAATG AAATCCGCAC GGCTTTCAAA 
GGCGCGCTGG ATGATTTGAA GTGGATGGAT GAACAGACCC GACAAGCTGC CAAGGACAAG 
GCGGATGCCA TCTATGACAT GATCGGATTT CCAGACTTCA TTCTGGACTC CAAAGAGCTG 
GATGATGTGT ATGATGGGTA TGAGGTTACG GAGGACAACT TCTTCCAGAA CATGATTAAC 
TTCTATAACT TCTCAGCACG AGTGATGGCA GATCAGCTCC GCAAGCCACC AAACAGAGAC 
CAATGGAGTA TGACTCCACC GACAGTGAAC GCTTACTACA TGCCAACCAA GAACGGAATC 
GTCTTTCCCG CTGGCATCTT ACAGGCCCCG TTCTACGCCC AGGATCACCC CAAAGCTTTA 
AACTTTGGTG GGATTGGAGT GGTCATGGGA CATGAGCTAA CGCATGCGTT TGATGACCAA 
GGCCGAGAGT ACGACAAAGA TGGAAACCTG CGCTCGTGGT GGCAGAACTC CTCGGTGGAG 
GCCTTCAAGA ACCGCACCGA GTGCATGGTC GATCAATACA CACAGTACAC CATCAACGGA 
GAGCACATTA ATGGAAAACA GACGCTGGGA GAAAACATCG CAGACAATGG CGGCCTGAAA 
GCTGCCTATC ATGCGTACAG GTCCTGGGTG CAGAAGAATG GGGAAGAGAA GAGACTCCCA 
GCTGTCAATC TGACAAACGA CCAGCTCTTC TTTGTGGGTT TTGCACAGGT GTGGTGCTCT 
GTCCGGACGC CAGAAAGCGC TCACGAGGGC CTGATGACGG ATCCTCACAG TCCGCCCAAA 
TACCGCGTGA TCGGCACCCT GTCTAACTCA CCCGAGTTCG CCGAGCACTT CCAATGTCCG 
CTGGGCTCCT CCATGAACTC AGGCCACCGC TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2B ( XM_017351042.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206531.1 Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55148
Clone ID Related Accession (Same CDS sequence)	XM_017351042.2 , XM_017351042.1
Accession Version	XM_017351042.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	endothelin-converting enzyme 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTAG CGCTGCAAGA TTTACGAAAT AACATGTCTA ACTACAAGCG TGCAACGTTT 
GAGGAGGAGG ACGGGACCGA TGTCCCTGTT GATGGAGCCA TTTCTCCTGA CAGTGTTGAG 
GTGGGTTTCC GTAAGGGTGG TATTCAGTTG TTTGGTCCAC TGGGCCGGAG GACTCAGCTG 
GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT TCACTTCTTG CACTGTTCGG CTGTGCGGTC 
ACACTGGGAG TCCGTTATAA CAGAGATCCA GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT 
GTCACTGTGG CCAGCAAGAT TGTTGAAGCT TTGGACCGCT CTGCCGACCC ATGTCAGGAT 
TTTTACCAAT ACGCCTGTGG AGGATGGGTC CGCAAAAACC CCCTGCCAGA TGGCCGGTCC 
CGCTGGAGCA CATTTAACAG CATCTGGGAC CAGAACCAGG CTGTGCTCAA ACACCTGCTG 
GAAAATGGCA CTTTTAACTC CAGCAGTGAA GCAGAGAGGA AGACACAGTC GTACTACCTT 
TCCTGTCTTA ATGAACAGCG AATAGAAGAA CTCGGAGCCC AACCACTCAT GGATTTGATT 
ACAAAGATCG GAGGCTGGAA CATCACAGAG TCCTGGGACA AAGAGAATTT TCTGGATGTT 
TTAAAGATCA TTTCTGGACC ATACAGAGCT CAGCCCTTCT TCACCGTCAG TGTTAGCGTA 
GACCCCAAAA ACTCCAACAG CAATGTGATT CAGGTCGACC AATCAGGACT CTTCCTCCCG 
TCCCGAGATT ATTACCTCAA TAAGACTAAC GAGAAGGTTT TGAAAGCTTA TCTGGATTAC 
ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA GACAAGAATT CCACTCGGGG TCAGATGCAG 
CAGATTTTGG ACTTTGAGAC GGCGCTGGCG AACATCACCG TGCCCCAGGA CGAACGGCGA 
GACGAGGAGA AGATCTACCA CAAGATAACC ATAGCTGACC TCCAGGTCTT GGCTCCGGCT 
ATTGAATGGT TGGATTATCT GAACTCAGTA CTTTCACCGC TGGAGCTGAA CGACACAGAA 
CCGGTGGTGG TGTATGCTAA AGAGTACATG CAACAAGTTT CAGAGCTCAT CAACAAGACC 
GACCACAGTC TTCTGAATAA TTACATGATC TGGAATCTGG TGCAGAAAGG AGCGTCCAGC 
CTTGATCAGC GCTTTGAAAA TGCTCAGGAT AAACTGCTGG AGAGCCTCTA TGGCACCAAA 
AAGTCTTGTA CTCCGCGCTG GCAGACGTGC ATTGGAAACA CAGACGACAC CCTCGGTTTT 
GCACTGGGAG CTCTTTTCGT CAAAGCCACC TTCGACAAAC AGAGCAAAGA AATAGACCTC 
AACGGTGGTG CGTTTTTGCC CTCATGTGGC CGTAATGTGA ACTCCACAGT TCACAACCTC 
ATGTTTTCTG TCATAACGCA GGCAGAGGGG ATGATCAATG AAATCCGCAC GGCTTTCAAA 
GGCGCGCTGG ATGATTTGAA GTGGATGGAT GAACAGACCC GACAAGCTGC CAAGGACAAG 
GCGGATGCCA TCTATGACAT GATCGGATTT CCAGACTTCA TTCTGGACTC CAAAGAGCTG 
GATGATGTGT ATGATGGGTA TGAGGTTACG GAGGACAACT TCTTCCAGAA CATGATTAAC 
TTCTATAACT TCTCAGCACG AGTGATGGCA GATCAGCTCC GCAAGCCACC AAACAGAGAC 
CAATGGAGTA TGACTCCACC GACAGTGAAC GCTTACTACA TGCCAACCAA GAACGGAATC 
GTCTTTCCCG CTGGCATCTT ACAGGCCCCG TTCTACGCCC AGGATCACCC CAAAGCTTTA 
AACTTTGGTG GGATTGGAGT GGTCATGGGA CATGAGCTAA CGCATGCGTT TGATGACCAA 
GGCCGAGAGT ACGACAAAGA TGGAAACCTG CGCTCGTGGT GGCAGAACTC CTCGGTGGAG 
GCCTTCAAGA ACCGCACCGA GTGCATGGTC GATCAATACA CACAGTACAC CATCAACGGA 
GAGCACATTA ATGGAAAACA GACGCTGGGA GAAAACATCG CAGACAATGG CGGCCTGAAA 
GCTGCCTATC ATGCGTACAG GTCCTGGGTG CAGAAGAATG GGGAAGAGAA GAGACTCCCA 
GCTGTCAATC TGACAAACGA CCAGCTCTTC TTTGTGGGTT TTGCACAGGT GTGGTGCTCT 
GTCCGGACGC CAGAAAGCGC TCACGAGGGC CTGATGACGG ATCCTCACAG TCCGCCCAAA 
TACCGCGTGA TCGGCACCCT GTCTAACTCA CCCGAGTTCG CCGAGCACTT CCAATGTCCG 
CTGGGCTCCT CCATGAACTC AGGCCACCGC TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206531.1
CDS	473..2857
Translation	MSVALQDLRNNMSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIDLNGGAFLPSCGRNVNSTVHNLMFSVITQAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	XM_017351042.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351042.1

ORF Insert Sequence:

ATGAGTGTAG CGCTGCAAGA TTTACGAAAT AACATGTCTA ACTACAAGCG TGCAACGTTT 
GAGGAGGAGG ACGGGACCGA TGTCCCTGTT GATGGAGCCA TTTCTCCTGA CAGTGTTGAG 
GTGGGTTTCC GTAAGGGTGG TATTCAGTTG TTTGGTCCAC TGGGCCGGAG GACTCAGCTG 
GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT TCACTTCTTG CACTGTTCGG CTGTGCGGTC 
ACACTGGGAG TCCGTTATAA CAGAGATCCA GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT 
GTCACTGTGG CCAGCAAGAT TGTTGAAGCT TTGGACCGCT CTGCCGACCC ATGTCAGGAT 
TTTTACCAAT ACGCCTGTGG AGGATGGGTC CGCAAAAACC CCCTGCCAGA TGGCCGGTCC 
CGCTGGAGCA CATTTAACAG CATCTGGGAC CAGAACCAGG CTGTGCTCAA ACACCTGCTG 
GAAAATGGCA CTTTTAACTC CAGCAGTGAA GCAGAGAGGA AGACACAGTC GTACTACCTT 
TCCTGTCTTA ATGAACAGCG AATAGAAGAA CTCGGAGCCC AACCACTCAT GGATTTGATT 
ACAAAGATCG GAGGCTGGAA CATCACAGAG TCCTGGGACA AAGAGAATTT TCTGGATGTT 
TTAAAGATCA TTTCTGGACC ATACAGAGCT CAGCCCTTCT TCACCGTCAG TGTTAGCGTA 
GACCCCAAAA ACTCCAACAG CAATGTGATT CAGGTCGACC AATCAGGACT CTTCCTCCCG 
TCCCGAGATT ATTACCTCAA TAAGACTAAC GAGAAGGTTT TGAAAGCTTA TCTGGATTAC 
ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA GACAAGAATT CCACTCGGGG TCAGATGCAG 
CAGATTTTGG ACTTTGAGAC GGCGCTGGCG AACATCACCG TGCCCCAGGA CGAACGGCGA 
GACGAGGAGA AGATCTACCA CAAGATAACC ATAGCTGACC TCCAGGTCTT GGCTCCGGCT 
ATTGAATGGT TGGATTATCT GAACTCAGTA CTTTCACCGC TGGAGCTGAA CGACACAGAA 
CCGGTGGTGG TGTATGCTAA AGAGTACATG CAACAAGTTT CAGAGCTCAT CAACAAGACC 
GACCACAGTC TTCTGAATAA TTACATGATC TGGAATCTGG TGCAGAAAGG AGCGTCCAGC 
CTTGATCAGC GCTTTGAAAA TGCTCAGGAT AAACTGCTGG AGAGCCTCTA TGGCACCAAA 
AAGTCTTGTA CTCCGCGCTG GCAGACGTGC ATTGGAAACA CAGACGACAC CCTCGGTTTT 
GCACTGGGAG CTCTTTTCGT CAAAGCCACC TTCGACAAAC AGAGCAAAGA AATAGACCTC 
AACGGTGGTG CGTTTTTGCC CTCATGTGGC CGTAATGTGA ACTCCACAGT TCACAACCTC 
ATGTTTTCTG TCATAACGCA GGCAGAGGGG ATGATCAATG AAATCCGCAC GGCTTTCAAA 
GGCGCGCTGG ATGATTTGAA GTGGATGGAT GAACAGACCC GACAAGCTGC CAAGGACAAG 
GCGGATGCCA TCTATGACAT GATCGGATTT CCAGACTTCA TTCTGGACTC CAAAGAGCTG 
GATGATGTGT ATGATGGGTA TGAGGTTACG GAGGACAACT TCTTCCAGAA CATGATTAAC 
TTCTATAACT TCTCAGCACG AGTGATGGCA GATCAGCTCC GCAAGCCACC AAACAGAGAC 
CAATGGAGTA TGACTCCACC GACAGTGAAC GCTTACTACA TGCCAACCAA GAACGGAATC 
GTCTTTCCCG CTGGCATCTT ACAGGCCCCG TTCTACGCCC AGGATCACCC CAAAGCTTTA 
AACTTTGGTG GGATTGGAGT GGTCATGGGA CATGAGCTAA CGCATGCGTT TGATGACCAA 
GGCCGAGAGT ACGACAAAGA TGGAAACCTG CGCTCGTGGT GGCAGAACTC CTCGGTGGAG 
GCCTTCAAGA ACCGCACCGA GTGCATGGTC GATCAATACA CACAGTACAC CATCAACGGA 
GAGCACATTA ATGGAAAACA GACGCTGGGA GAAAACATCG CAGACAATGG CGGCCTGAAA 
GCTGCCTATC ATGCGTACAG GTCCTGGGTG CAGAAGAATG GGGAAGAGAA GAGACTCCCA 
GCTGTCAATC TGACAAACGA CCAGCTCTTC TTTGTGGGTT TTGCACAGGT GTGGTGCTCT 
GTCCGGACGC CAGAAAGCGC TCACGAGGGC CTGATGACGG ATCCTCACAG TCCGCCCAAA 
TACCGCGTGA TCGGCACCCT GTCTAACTCA CCCGAGTTCG CCGAGCACTT CCAATGTCCG 
CTGGGCTCCT CCATGAACTC AGGCCACCGC TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2B ( XM_017351043.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206532.1 Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa55149
Clone ID Related Accession (Same CDS sequence)	XM_017351043.1 , XM_017351043.2
Accession Version	XM_017351043.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	endothelin-converting enzyme 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTA ACTACAAGCG TGCAACGTTT GAGGAGGAGG ACGGGACCGA TGTCCCTGTT 
GATGGAGCCA TTTCTCCTGA CAGTGTTGAG GTGGGTTTCC GTAAGGGTGG TATTCAGTTG 
TTTGGTCCAC TGGGCCGGAG GACTCAGCTG GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT 
TCACTTCTTG CACTGTTCGG CTGTGCGGTC ACACTGGGAG TCCGTTATAA CAGAGATCCA 
GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT GTCACTGTGG CCAGCAAGAT TGTTGAAGCT 
TTGGACCGCT CTGCCGACCC ATGTCAGGAT TTTTACCAAT ACGCCTGTGG AGGATGGGTC 
CGCAAAAACC CCCTGCCAGA TGGCCGGTCC CGCTGGAGCA CATTTAACAG CATCTGGGAC 
CAGAACCAGG CTGTGCTCAA ACACCTGCTG GAAAATGGCA CTTTTAACTC CAGCAGTGAA 
GCAGAGAGGA AGACACAGTC GTACTACCTT TCCTGTCTTA ATGAACAGCG AATAGAAGAA 
CTCGGAGCCC AACCACTCAT GGATTTGATT ACAAAGATCG GAGGCTGGAA CATCACAGAG 
TCCTGGGACA AAGAGAATTT TCTGGATGTT TTAAAGATCA TTTCTGGACC ATACAGAGCT 
CAGCCCTTCT TCACCGTCAG TGTTAGCGTA GACCCCAAAA ACTCCAACAG CAATGTGATT 
CAGGTCGACC AATCAGGACT CTTCCTCCCG TCCCGAGATT ATTACCTCAA TAAGACTAAC 
GAGAAGGTTT TGAAAGCTTA TCTGGATTAC ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA 
GACAAGAATT CCACTCGGGG TCAGATGCAG CAGATTTTGG ACTTTGAGAC GGCGCTGGCG 
AACATCACCG TGCCCCAGGA CGAACGGCGA GACGAGGAGA AGATCTACCA CAAGATAACC 
ATAGCTGACC TCCAGGTCTT GGCTCCGGCT ATTGAATGGT TGGATTATCT GAACTCAGTA 
CTTTCACCGC TGGAGCTGAA CGACACAGAA CCGGTGGTGG TGTATGCTAA AGAGTACATG 
CAACAAGTTT CAGAGCTCAT CAACAAGACC GACCACAGTC TTCTGAATAA TTACATGATC 
TGGAATCTGG TGCAGAAAGG AGCGTCCAGC CTTGATCAGC GCTTTGAAAA TGCTCAGGAT 
AAACTGCTGG AGAGCCTCTA TGGCACCAAA AAGTCTTGTA CTCCGCGCTG GCAGACGTGC 
ATTGGAAACA CAGACGACAC CCTCGGTTTT GCACTGGGAG CTCTTTTCGT CAAAGCCACC 
TTCGACAAAC AGAGCAAAGA AATAGACCTC AACGGTGGTG CGTTTTTGCC CTCATGTGGC 
CGTAATGTGA ACTCCACAGT TCACAACCTC ATGTTTTCTG TCATAACGCA GGCAGAGGGG 
ATGATCAATG AAATCCGCAC GGCTTTCAAA GGCGCGCTGG ATGATTTGAA GTGGATGGAT 
GAACAGACCC GACAAGCTGC CAAGGACAAG GCGGATGCCA TCTATGACAT GATCGGATTT 
CCAGACTTCA TTCTGGACTC CAAAGAGCTG GATGATGTGT ATGATGGGTA TGAGGTTACG 
GAGGACAACT TCTTCCAGAA CATGATTAAC TTCTATAACT TCTCAGCACG AGTGATGGCA 
GATCAGCTCC GCAAGCCACC AAACAGAGAC CAATGGAGTA TGACTCCACC GACAGTGAAC 
GCTTACTACA TGCCAACCAA GAACGGAATC GTCTTTCCCG CTGGCATCTT ACAGGCCCCG 
TTCTACGCCC AGGATCACCC CAAAGCTTTA AACTTTGGTG GGATTGGAGT GGTCATGGGA 
CATGAGCTAA CGCATGCGTT TGATGACCAA GGCCGAGAGT ACGACAAAGA TGGAAACCTG 
CGCTCGTGGT GGCAGAACTC CTCGGTGGAG GCCTTCAAGA ACCGCACCGA GTGCATGGTC 
GATCAATACA CACAGTACAC CATCAACGGA GAGCACATTA ATGGAAAACA GACGCTGGGA 
GAAAACATCG CAGACAATGG CGGCCTGAAA GCTGCCTATC ATGCGTACAG GTCCTGGGTG 
CAGAAGAATG GGGAAGAGAA GAGACTCCCA GCTGTCAATC TGACAAACGA CCAGCTCTTC 
TTTGTGGGTT TTGCACAGGT GTGGTGCTCT GTCCGGACGC CAGAAAGCGC TCACGAGGGC 
CTGATGACGG ATCCTCACAG TCCGCCCAAA TACCGCGTGA TCGGCACCCT GTCTAACTCA 
CCCGAGTTCG CCGAGCACTT CCAATGTCCG CTGGGCTCCT CCATGAACTC AGGCCACCGC 
TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206532.1
CDS	188..2542
Translation	MMSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIDLNGGAFLPSCGRNVNSTVHNLMFSVITQAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	XM_017351043.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351043.1

ORF Insert Sequence:

ATGATGTCTA ACTACAAGCG TGCAACGTTT GAGGAGGAGG ACGGGACCGA TGTCCCTGTT 
GATGGAGCCA TTTCTCCTGA CAGTGTTGAG GTGGGTTTCC GTAAGGGTGG TATTCAGTTG 
TTTGGTCCAC TGGGCCGGAG GACTCAGCTG GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT 
TCACTTCTTG CACTGTTCGG CTGTGCGGTC ACACTGGGAG TCCGTTATAA CAGAGATCCA 
GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT GTCACTGTGG CCAGCAAGAT TGTTGAAGCT 
TTGGACCGCT CTGCCGACCC ATGTCAGGAT TTTTACCAAT ACGCCTGTGG AGGATGGGTC 
CGCAAAAACC CCCTGCCAGA TGGCCGGTCC CGCTGGAGCA CATTTAACAG CATCTGGGAC 
CAGAACCAGG CTGTGCTCAA ACACCTGCTG GAAAATGGCA CTTTTAACTC CAGCAGTGAA 
GCAGAGAGGA AGACACAGTC GTACTACCTT TCCTGTCTTA ATGAACAGCG AATAGAAGAA 
CTCGGAGCCC AACCACTCAT GGATTTGATT ACAAAGATCG GAGGCTGGAA CATCACAGAG 
TCCTGGGACA AAGAGAATTT TCTGGATGTT TTAAAGATCA TTTCTGGACC ATACAGAGCT 
CAGCCCTTCT TCACCGTCAG TGTTAGCGTA GACCCCAAAA ACTCCAACAG CAATGTGATT 
CAGGTCGACC AATCAGGACT CTTCCTCCCG TCCCGAGATT ATTACCTCAA TAAGACTAAC 
GAGAAGGTTT TGAAAGCTTA TCTGGATTAC ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA 
GACAAGAATT CCACTCGGGG TCAGATGCAG CAGATTTTGG ACTTTGAGAC GGCGCTGGCG 
AACATCACCG TGCCCCAGGA CGAACGGCGA GACGAGGAGA AGATCTACCA CAAGATAACC 
ATAGCTGACC TCCAGGTCTT GGCTCCGGCT ATTGAATGGT TGGATTATCT GAACTCAGTA 
CTTTCACCGC TGGAGCTGAA CGACACAGAA CCGGTGGTGG TGTATGCTAA AGAGTACATG 
CAACAAGTTT CAGAGCTCAT CAACAAGACC GACCACAGTC TTCTGAATAA TTACATGATC 
TGGAATCTGG TGCAGAAAGG AGCGTCCAGC CTTGATCAGC GCTTTGAAAA TGCTCAGGAT 
AAACTGCTGG AGAGCCTCTA TGGCACCAAA AAGTCTTGTA CTCCGCGCTG GCAGACGTGC 
ATTGGAAACA CAGACGACAC CCTCGGTTTT GCACTGGGAG CTCTTTTCGT CAAAGCCACC 
TTCGACAAAC AGAGCAAAGA AATAGACCTC AACGGTGGTG CGTTTTTGCC CTCATGTGGC 
CGTAATGTGA ACTCCACAGT TCACAACCTC ATGTTTTCTG TCATAACGCA GGCAGAGGGG 
ATGATCAATG AAATCCGCAC GGCTTTCAAA GGCGCGCTGG ATGATTTGAA GTGGATGGAT 
GAACAGACCC GACAAGCTGC CAAGGACAAG GCGGATGCCA TCTATGACAT GATCGGATTT 
CCAGACTTCA TTCTGGACTC CAAAGAGCTG GATGATGTGT ATGATGGGTA TGAGGTTACG 
GAGGACAACT TCTTCCAGAA CATGATTAAC TTCTATAACT TCTCAGCACG AGTGATGGCA 
GATCAGCTCC GCAAGCCACC AAACAGAGAC CAATGGAGTA TGACTCCACC GACAGTGAAC 
GCTTACTACA TGCCAACCAA GAACGGAATC GTCTTTCCCG CTGGCATCTT ACAGGCCCCG 
TTCTACGCCC AGGATCACCC CAAAGCTTTA AACTTTGGTG GGATTGGAGT GGTCATGGGA 
CATGAGCTAA CGCATGCGTT TGATGACCAA GGCCGAGAGT ACGACAAAGA TGGAAACCTG 
CGCTCGTGGT GGCAGAACTC CTCGGTGGAG GCCTTCAAGA ACCGCACCGA GTGCATGGTC 
GATCAATACA CACAGTACAC CATCAACGGA GAGCACATTA ATGGAAAACA GACGCTGGGA 
GAAAACATCG CAGACAATGG CGGCCTGAAA GCTGCCTATC ATGCGTACAG GTCCTGGGTG 
CAGAAGAATG GGGAAGAGAA GAGACTCCCA GCTGTCAATC TGACAAACGA CCAGCTCTTC 
TTTGTGGGTT TTGCACAGGT GTGGTGCTCT GTCCGGACGC CAGAAAGCGC TCACGAGGGC 
CTGATGACGG ATCCTCACAG TCCGCCCAAA TACCGCGTGA TCGGCACCCT GTCTAACTCA 
CCCGAGTTCG CCGAGCACTT CCAATGTCCG CTGGGCTCCT CCATGAACTC AGGCCACCGC 
TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2B ( XM_017351043.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206532.1 Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa55149
Clone ID Related Accession (Same CDS sequence)	XM_017351043.1 , XM_017351043.2
Accession Version	XM_017351043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endothelin-converting enzyme 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTA ACTACAAGCG TGCAACGTTT GAGGAGGAGG ACGGGACCGA TGTCCCTGTT 
GATGGAGCCA TTTCTCCTGA CAGTGTTGAG GTGGGTTTCC GTAAGGGTGG TATTCAGTTG 
TTTGGTCCAC TGGGCCGGAG GACTCAGCTG GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT 
TCACTTCTTG CACTGTTCGG CTGTGCGGTC ACACTGGGAG TCCGTTATAA CAGAGATCCA 
GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT GTCACTGTGG CCAGCAAGAT TGTTGAAGCT 
TTGGACCGCT CTGCCGACCC ATGTCAGGAT TTTTACCAAT ACGCCTGTGG AGGATGGGTC 
CGCAAAAACC CCCTGCCAGA TGGCCGGTCC CGCTGGAGCA CATTTAACAG CATCTGGGAC 
CAGAACCAGG CTGTGCTCAA ACACCTGCTG GAAAATGGCA CTTTTAACTC CAGCAGTGAA 
GCAGAGAGGA AGACACAGTC GTACTACCTT TCCTGTCTTA ATGAACAGCG AATAGAAGAA 
CTCGGAGCCC AACCACTCAT GGATTTGATT ACAAAGATCG GAGGCTGGAA CATCACAGAG 
TCCTGGGACA AAGAGAATTT TCTGGATGTT TTAAAGATCA TTTCTGGACC ATACAGAGCT 
CAGCCCTTCT TCACCGTCAG TGTTAGCGTA GACCCCAAAA ACTCCAACAG CAATGTGATT 
CAGGTCGACC AATCAGGACT CTTCCTCCCG TCCCGAGATT ATTACCTCAA TAAGACTAAC 
GAGAAGGTTT TGAAAGCTTA TCTGGATTAC ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA 
GACAAGAATT CCACTCGGGG TCAGATGCAG CAGATTTTGG ACTTTGAGAC GGCGCTGGCG 
AACATCACCG TGCCCCAGGA CGAACGGCGA GACGAGGAGA AGATCTACCA CAAGATAACC 
ATAGCTGACC TCCAGGTCTT GGCTCCGGCT ATTGAATGGT TGGATTATCT GAACTCAGTA 
CTTTCACCGC TGGAGCTGAA CGACACAGAA CCGGTGGTGG TGTATGCTAA AGAGTACATG 
CAACAAGTTT CAGAGCTCAT CAACAAGACC GACCACAGTC TTCTGAATAA TTACATGATC 
TGGAATCTGG TGCAGAAAGG AGCGTCCAGC CTTGATCAGC GCTTTGAAAA TGCTCAGGAT 
AAACTGCTGG AGAGCCTCTA TGGCACCAAA AAGTCTTGTA CTCCGCGCTG GCAGACGTGC 
ATTGGAAACA CAGACGACAC CCTCGGTTTT GCACTGGGAG CTCTTTTCGT CAAAGCCACC 
TTCGACAAAC AGAGCAAAGA AATAGACCTC AACGGTGGTG CGTTTTTGCC CTCATGTGGC 
CGTAATGTGA ACTCCACAGT TCACAACCTC ATGTTTTCTG TCATAACGCA GGCAGAGGGG 
ATGATCAATG AAATCCGCAC GGCTTTCAAA GGCGCGCTGG ATGATTTGAA GTGGATGGAT 
GAACAGACCC GACAAGCTGC CAAGGACAAG GCGGATGCCA TCTATGACAT GATCGGATTT 
CCAGACTTCA TTCTGGACTC CAAAGAGCTG GATGATGTGT ATGATGGGTA TGAGGTTACG 
GAGGACAACT TCTTCCAGAA CATGATTAAC TTCTATAACT TCTCAGCACG AGTGATGGCA 
GATCAGCTCC GCAAGCCACC AAACAGAGAC CAATGGAGTA TGACTCCACC GACAGTGAAC 
GCTTACTACA TGCCAACCAA GAACGGAATC GTCTTTCCCG CTGGCATCTT ACAGGCCCCG 
TTCTACGCCC AGGATCACCC CAAAGCTTTA AACTTTGGTG GGATTGGAGT GGTCATGGGA 
CATGAGCTAA CGCATGCGTT TGATGACCAA GGCCGAGAGT ACGACAAAGA TGGAAACCTG 
CGCTCGTGGT GGCAGAACTC CTCGGTGGAG GCCTTCAAGA ACCGCACCGA GTGCATGGTC 
GATCAATACA CACAGTACAC CATCAACGGA GAGCACATTA ATGGAAAACA GACGCTGGGA 
GAAAACATCG CAGACAATGG CGGCCTGAAA GCTGCCTATC ATGCGTACAG GTCCTGGGTG 
CAGAAGAATG GGGAAGAGAA GAGACTCCCA GCTGTCAATC TGACAAACGA CCAGCTCTTC 
TTTGTGGGTT TTGCACAGGT GTGGTGCTCT GTCCGGACGC CAGAAAGCGC TCACGAGGGC 
CTGATGACGG ATCCTCACAG TCCGCCCAAA TACCGCGTGA TCGGCACCCT GTCTAACTCA 
CCCGAGTTCG CCGAGCACTT CCAATGTCCG CTGGGCTCCT CCATGAACTC AGGCCACCGC 
TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206532.1
CDS	978..3332
Translation	MMSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIDLNGGAFLPSCGRNVNSTVHNLMFSVITQAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	XM_017351043.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351043.2

ORF Insert Sequence:

ATGATGTCTA ACTACAAGCG TGCAACGTTT GAGGAGGAGG ACGGGACCGA TGTCCCTGTT 
GATGGAGCCA TTTCTCCTGA CAGTGTTGAG GTGGGTTTCC GTAAGGGTGG TATTCAGTTG 
TTTGGTCCAC TGGGCCGGAG GACTCAGCTG GAGGTAGTGT TGGCCGGTCT GCTTCTTGCT 
TCACTTCTTG CACTGTTCGG CTGTGCGGTC ACACTGGGAG TCCGTTATAA CAGAGATCCA 
GCCCGTAGTA TCTGTCTAAC CGAGGCCTGT GTCACTGTGG CCAGCAAGAT TGTTGAAGCT 
TTGGACCGCT CTGCCGACCC ATGTCAGGAT TTTTACCAAT ACGCCTGTGG AGGATGGGTC 
CGCAAAAACC CCCTGCCAGA TGGCCGGTCC CGCTGGAGCA CATTTAACAG CATCTGGGAC 
CAGAACCAGG CTGTGCTCAA ACACCTGCTG GAAAATGGCA CTTTTAACTC CAGCAGTGAA 
GCAGAGAGGA AGACACAGTC GTACTACCTT TCCTGTCTTA ATGAACAGCG AATAGAAGAA 
CTCGGAGCCC AACCACTCAT GGATTTGATT ACAAAGATCG GAGGCTGGAA CATCACAGAG 
TCCTGGGACA AAGAGAATTT TCTGGATGTT TTAAAGATCA TTTCTGGACC ATACAGAGCT 
CAGCCCTTCT TCACCGTCAG TGTTAGCGTA GACCCCAAAA ACTCCAACAG CAATGTGATT 
CAGGTCGACC AATCAGGACT CTTCCTCCCG TCCCGAGATT ATTACCTCAA TAAGACTAAC 
GAGAAGGTTT TGAAAGCTTA TCTGGATTAC ATGGTGGAGC TGGGTTTGTT GTTGGGAGGA 
GACAAGAATT CCACTCGGGG TCAGATGCAG CAGATTTTGG ACTTTGAGAC GGCGCTGGCG 
AACATCACCG TGCCCCAGGA CGAACGGCGA GACGAGGAGA AGATCTACCA CAAGATAACC 
ATAGCTGACC TCCAGGTCTT GGCTCCGGCT ATTGAATGGT TGGATTATCT GAACTCAGTA 
CTTTCACCGC TGGAGCTGAA CGACACAGAA CCGGTGGTGG TGTATGCTAA AGAGTACATG 
CAACAAGTTT CAGAGCTCAT CAACAAGACC GACCACAGTC TTCTGAATAA TTACATGATC 
TGGAATCTGG TGCAGAAAGG AGCGTCCAGC CTTGATCAGC GCTTTGAAAA TGCTCAGGAT 
AAACTGCTGG AGAGCCTCTA TGGCACCAAA AAGTCTTGTA CTCCGCGCTG GCAGACGTGC 
ATTGGAAACA CAGACGACAC CCTCGGTTTT GCACTGGGAG CTCTTTTCGT CAAAGCCACC 
TTCGACAAAC AGAGCAAAGA AATAGACCTC AACGGTGGTG CGTTTTTGCC CTCATGTGGC 
CGTAATGTGA ACTCCACAGT TCACAACCTC ATGTTTTCTG TCATAACGCA GGCAGAGGGG 
ATGATCAATG AAATCCGCAC GGCTTTCAAA GGCGCGCTGG ATGATTTGAA GTGGATGGAT 
GAACAGACCC GACAAGCTGC CAAGGACAAG GCGGATGCCA TCTATGACAT GATCGGATTT 
CCAGACTTCA TTCTGGACTC CAAAGAGCTG GATGATGTGT ATGATGGGTA TGAGGTTACG 
GAGGACAACT TCTTCCAGAA CATGATTAAC TTCTATAACT TCTCAGCACG AGTGATGGCA 
GATCAGCTCC GCAAGCCACC AAACAGAGAC CAATGGAGTA TGACTCCACC GACAGTGAAC 
GCTTACTACA TGCCAACCAA GAACGGAATC GTCTTTCCCG CTGGCATCTT ACAGGCCCCG 
TTCTACGCCC AGGATCACCC CAAAGCTTTA AACTTTGGTG GGATTGGAGT GGTCATGGGA 
CATGAGCTAA CGCATGCGTT TGATGACCAA GGCCGAGAGT ACGACAAAGA TGGAAACCTG 
CGCTCGTGGT GGCAGAACTC CTCGGTGGAG GCCTTCAAGA ACCGCACCGA GTGCATGGTC 
GATCAATACA CACAGTACAC CATCAACGGA GAGCACATTA ATGGAAAACA GACGCTGGGA 
GAAAACATCG CAGACAATGG CGGCCTGAAA GCTGCCTATC ATGCGTACAG GTCCTGGGTG 
CAGAAGAATG GGGAAGAGAA GAGACTCCCA GCTGTCAATC TGACAAACGA CCAGCTCTTC 
TTTGTGGGTT TTGCACAGGT GTGGTGCTCT GTCCGGACGC CAGAAAGCGC TCACGAGGGC 
CTGATGACGG ATCCTCACAG TCCGCCCAAA TACCGCGTGA TCGGCACCCT GTCTAACTCA 
CCCGAGTTCG CCGAGCACTT CCAATGTCCG CTGGGCTCCT CCATGAACTC AGGCCACCGC 
TGCGAGGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2B ( XM_017351044.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206533.1 Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa55150
Clone ID Related Accession (Same CDS sequence)	XM_017351044.1 , XM_017351044.2
Accession Version	XM_017351044.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	endothelin-converting enzyme 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACT ACAAGCGTGC AACGTTTGAG GAGGAGGACG GGACCGATGT CCCTGTTGAT 
GGAGCCATTT CTCCTGACAG TGTTGAGGTG GGTTTCCGTA AGGGTGGTAT TCAGTTGTTT 
GGTCCACTGG GCCGGAGGAC TCAGCTGGAG GTAGTGTTGG CCGGTCTGCT TCTTGCTTCA 
CTTCTTGCAC TGTTCGGCTG TGCGGTCACA CTGGGAGTCC GTTATAACAG AGATCCAGCC 
CGTAGTATCT GTCTAACCGA GGCCTGTGTC ACTGTGGCCA GCAAGATTGT TGAAGCTTTG 
GACCGCTCTG CCGACCCATG TCAGGATTTT TACCAATACG CCTGTGGAGG ATGGGTCCGC 
AAAAACCCCC TGCCAGATGG CCGGTCCCGC TGGAGCACAT TTAACAGCAT CTGGGACCAG 
AACCAGGCTG TGCTCAAACA CCTGCTGGAA AATGGCACTT TTAACTCCAG CAGTGAAGCA 
GAGAGGAAGA CACAGTCGTA CTACCTTTCC TGTCTTAATG AACAGCGAAT AGAAGAACTC 
GGAGCCCAAC CACTCATGGA TTTGATTACA AAGATCGGAG GCTGGAACAT CACAGAGTCC 
TGGGACAAAG AGAATTTTCT GGATGTTTTA AAGATCATTT CTGGACCATA CAGAGCTCAG 
CCCTTCTTCA CCGTCAGTGT TAGCGTAGAC CCCAAAAACT CCAACAGCAA TGTGATTCAG 
GTCGACCAAT CAGGACTCTT CCTCCCGTCC CGAGATTATT ACCTCAATAA GACTAACGAG 
AAGGTTTTGA AAGCTTATCT GGATTACATG GTGGAGCTGG GTTTGTTGTT GGGAGGAGAC 
AAGAATTCCA CTCGGGGTCA GATGCAGCAG ATTTTGGACT TTGAGACGGC GCTGGCGAAC 
ATCACCGTGC CCCAGGACGA ACGGCGAGAC GAGGAGAAGA TCTACCACAA GATAACCATA 
GCTGACCTCC AGGTCTTGGC TCCGGCTATT GAATGGTTGG ATTATCTGAA CTCAGTACTT 
TCACCGCTGG AGCTGAACGA CACAGAACCG GTGGTGGTGT ATGCTAAAGA GTACATGCAA 
CAAGTTTCAG AGCTCATCAA CAAGACCGAC CACAGTCTTC TGAATAATTA CATGATCTGG 
AATCTGGTGC AGAAAGGAGC GTCCAGCCTT GATCAGCGCT TTGAAAATGC TCAGGATAAA 
CTGCTGGAGA GCCTCTATGG CACCAAAAAG TCTTGTACTC CGCGCTGGCA GACGTGCATT 
GGAAACACAG ACGACACCCT CGGTTTTGCA CTGGGAGCTC TTTTCGTCAA AGCCACCTTC 
GACAAACAGA GCAAAGAAAT AGACCTCAAC GGTGGTGCGT TTTTGCCCTC ATGTGGCCGT 
AATGTGAACT CCACAGTTCA CAACCTCATG TTTTCTGTCA TAACGCAGGC AGAGGGGATG 
ATCAATGAAA TCCGCACGGC TTTCAAAGGC GCGCTGGATG ATTTGAAGTG GATGGATGAA 
CAGACCCGAC AAGCTGCCAA GGACAAGGCG GATGCCATCT ATGACATGAT CGGATTTCCA 
GACTTCATTC TGGACTCCAA AGAGCTGGAT GATGTGTATG ATGGGTATGA GGTTACGGAG 
GACAACTTCT TCCAGAACAT GATTAACTTC TATAACTTCT CAGCACGAGT GATGGCAGAT 
CAGCTCCGCA AGCCACCAAA CAGAGACCAA TGGAGTATGA CTCCACCGAC AGTGAACGCT 
TACTACATGC CAACCAAGAA CGGAATCGTC TTTCCCGCTG GCATCTTACA GGCCCCGTTC 
TACGCCCAGG ATCACCCCAA AGCTTTAAAC TTTGGTGGGA TTGGAGTGGT CATGGGACAT 
GAGCTAACGC ATGCGTTTGA TGACCAAGGC CGAGAGTACG ACAAAGATGG AAACCTGCGC 
TCGTGGTGGC AGAACTCCTC GGTGGAGGCC TTCAAGAACC GCACCGAGTG CATGGTCGAT 
CAATACACAC AGTACACCAT CAACGGAGAG CACATTAATG GAAAACAGAC GCTGGGAGAA 
AACATCGCAG ACAATGGCGG CCTGAAAGCT GCCTATCATG CGTACAGGTC CTGGGTGCAG 
AAGAATGGGG AAGAGAAGAG ACTCCCAGCT GTCAATCTGA CAAACGACCA GCTCTTCTTT 
GTGGGTTTTG CACAGGTGTG GTGCTCTGTC CGGACGCCAG AAAGCGCTCA CGAGGGCCTG 
ATGACGGATC CTCACAGTCC GCCCAAATAC CGCGTGATCG GCACCCTGTC TAACTCACCC 
GAGTTCGCCG AGCACTTCCA ATGTCCGCTG GGCTCCTCCA TGAACTCAGG CCACCGCTGC 
GAGGTTTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206533.1
CDS	150..2501
Translation	MSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIDLNGGAFLPSCGRNVNSTVHNLMFSVITQAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	XM_017351044.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351044.1

ORF Insert Sequence:

ATGTCTAACT ACAAGCGTGC AACGTTTGAG GAGGAGGACG GGACCGATGT CCCTGTTGAT 
GGAGCCATTT CTCCTGACAG TGTTGAGGTG GGTTTCCGTA AGGGTGGTAT TCAGTTGTTT 
GGTCCACTGG GCCGGAGGAC TCAGCTGGAG GTAGTGTTGG CCGGTCTGCT TCTTGCTTCA 
CTTCTTGCAC TGTTCGGCTG TGCGGTCACA CTGGGAGTCC GTTATAACAG AGATCCAGCC 
CGTAGTATCT GTCTAACCGA GGCCTGTGTC ACTGTGGCCA GCAAGATTGT TGAAGCTTTG 
GACCGCTCTG CCGACCCATG TCAGGATTTT TACCAATACG CCTGTGGAGG ATGGGTCCGC 
AAAAACCCCC TGCCAGATGG CCGGTCCCGC TGGAGCACAT TTAACAGCAT CTGGGACCAG 
AACCAGGCTG TGCTCAAACA CCTGCTGGAA AATGGCACTT TTAACTCCAG CAGTGAAGCA 
GAGAGGAAGA CACAGTCGTA CTACCTTTCC TGTCTTAATG AACAGCGAAT AGAAGAACTC 
GGAGCCCAAC CACTCATGGA TTTGATTACA AAGATCGGAG GCTGGAACAT CACAGAGTCC 
TGGGACAAAG AGAATTTTCT GGATGTTTTA AAGATCATTT CTGGACCATA CAGAGCTCAG 
CCCTTCTTCA CCGTCAGTGT TAGCGTAGAC CCCAAAAACT CCAACAGCAA TGTGATTCAG 
GTCGACCAAT CAGGACTCTT CCTCCCGTCC CGAGATTATT ACCTCAATAA GACTAACGAG 
AAGGTTTTGA AAGCTTATCT GGATTACATG GTGGAGCTGG GTTTGTTGTT GGGAGGAGAC 
AAGAATTCCA CTCGGGGTCA GATGCAGCAG ATTTTGGACT TTGAGACGGC GCTGGCGAAC 
ATCACCGTGC CCCAGGACGA ACGGCGAGAC GAGGAGAAGA TCTACCACAA GATAACCATA 
GCTGACCTCC AGGTCTTGGC TCCGGCTATT GAATGGTTGG ATTATCTGAA CTCAGTACTT 
TCACCGCTGG AGCTGAACGA CACAGAACCG GTGGTGGTGT ATGCTAAAGA GTACATGCAA 
CAAGTTTCAG AGCTCATCAA CAAGACCGAC CACAGTCTTC TGAATAATTA CATGATCTGG 
AATCTGGTGC AGAAAGGAGC GTCCAGCCTT GATCAGCGCT TTGAAAATGC TCAGGATAAA 
CTGCTGGAGA GCCTCTATGG CACCAAAAAG TCTTGTACTC CGCGCTGGCA GACGTGCATT 
GGAAACACAG ACGACACCCT CGGTTTTGCA CTGGGAGCTC TTTTCGTCAA AGCCACCTTC 
GACAAACAGA GCAAAGAAAT AGACCTCAAC GGTGGTGCGT TTTTGCCCTC ATGTGGCCGT 
AATGTGAACT CCACAGTTCA CAACCTCATG TTTTCTGTCA TAACGCAGGC AGAGGGGATG 
ATCAATGAAA TCCGCACGGC TTTCAAAGGC GCGCTGGATG ATTTGAAGTG GATGGATGAA 
CAGACCCGAC AAGCTGCCAA GGACAAGGCG GATGCCATCT ATGACATGAT CGGATTTCCA 
GACTTCATTC TGGACTCCAA AGAGCTGGAT GATGTGTATG ATGGGTATGA GGTTACGGAG 
GACAACTTCT TCCAGAACAT GATTAACTTC TATAACTTCT CAGCACGAGT GATGGCAGAT 
CAGCTCCGCA AGCCACCAAA CAGAGACCAA TGGAGTATGA CTCCACCGAC AGTGAACGCT 
TACTACATGC CAACCAAGAA CGGAATCGTC TTTCCCGCTG GCATCTTACA GGCCCCGTTC 
TACGCCCAGG ATCACCCCAA AGCTTTAAAC TTTGGTGGGA TTGGAGTGGT CATGGGACAT 
GAGCTAACGC ATGCGTTTGA TGACCAAGGC CGAGAGTACG ACAAAGATGG AAACCTGCGC 
TCGTGGTGGC AGAACTCCTC GGTGGAGGCC TTCAAGAACC GCACCGAGTG CATGGTCGAT 
CAATACACAC AGTACACCAT CAACGGAGAG CACATTAATG GAAAACAGAC GCTGGGAGAA 
AACATCGCAG ACAATGGCGG CCTGAAAGCT GCCTATCATG CGTACAGGTC CTGGGTGCAG 
AAGAATGGGG AAGAGAAGAG ACTCCCAGCT GTCAATCTGA CAAACGACCA GCTCTTCTTT 
GTGGGTTTTG CACAGGTGTG GTGCTCTGTC CGGACGCCAG AAAGCGCTCA CGAGGGCCTG 
ATGACGGATC CTCACAGTCC GCCCAAATAC CGCGTGATCG GCACCCTGTC TAACTCACCC 
GAGTTCGCCG AGCACTTCCA ATGTCCGCTG GGCTCCTCCA TGAACTCAGG CCACCGCTGC 
GAGGTTTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2B ( XM_017351044.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206533.1 Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa55150
Clone ID Related Accession (Same CDS sequence)	XM_017351044.1 , XM_017351044.2
Accession Version	XM_017351044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	endothelin-converting enzyme 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAACT ACAAGCGTGC AACGTTTGAG GAGGAGGACG GGACCGATGT CCCTGTTGAT 
GGAGCCATTT CTCCTGACAG TGTTGAGGTG GGTTTCCGTA AGGGTGGTAT TCAGTTGTTT 
GGTCCACTGG GCCGGAGGAC TCAGCTGGAG GTAGTGTTGG CCGGTCTGCT TCTTGCTTCA 
CTTCTTGCAC TGTTCGGCTG TGCGGTCACA CTGGGAGTCC GTTATAACAG AGATCCAGCC 
CGTAGTATCT GTCTAACCGA GGCCTGTGTC ACTGTGGCCA GCAAGATTGT TGAAGCTTTG 
GACCGCTCTG CCGACCCATG TCAGGATTTT TACCAATACG CCTGTGGAGG ATGGGTCCGC 
AAAAACCCCC TGCCAGATGG CCGGTCCCGC TGGAGCACAT TTAACAGCAT CTGGGACCAG 
AACCAGGCTG TGCTCAAACA CCTGCTGGAA AATGGCACTT TTAACTCCAG CAGTGAAGCA 
GAGAGGAAGA CACAGTCGTA CTACCTTTCC TGTCTTAATG AACAGCGAAT AGAAGAACTC 
GGAGCCCAAC CACTCATGGA TTTGATTACA AAGATCGGAG GCTGGAACAT CACAGAGTCC 
TGGGACAAAG AGAATTTTCT GGATGTTTTA AAGATCATTT CTGGACCATA CAGAGCTCAG 
CCCTTCTTCA CCGTCAGTGT TAGCGTAGAC CCCAAAAACT CCAACAGCAA TGTGATTCAG 
GTCGACCAAT CAGGACTCTT CCTCCCGTCC CGAGATTATT ACCTCAATAA GACTAACGAG 
AAGGTTTTGA AAGCTTATCT GGATTACATG GTGGAGCTGG GTTTGTTGTT GGGAGGAGAC 
AAGAATTCCA CTCGGGGTCA GATGCAGCAG ATTTTGGACT TTGAGACGGC GCTGGCGAAC 
ATCACCGTGC CCCAGGACGA ACGGCGAGAC GAGGAGAAGA TCTACCACAA GATAACCATA 
GCTGACCTCC AGGTCTTGGC TCCGGCTATT GAATGGTTGG ATTATCTGAA CTCAGTACTT 
TCACCGCTGG AGCTGAACGA CACAGAACCG GTGGTGGTGT ATGCTAAAGA GTACATGCAA 
CAAGTTTCAG AGCTCATCAA CAAGACCGAC CACAGTCTTC TGAATAATTA CATGATCTGG 
AATCTGGTGC AGAAAGGAGC GTCCAGCCTT GATCAGCGCT TTGAAAATGC TCAGGATAAA 
CTGCTGGAGA GCCTCTATGG CACCAAAAAG TCTTGTACTC CGCGCTGGCA GACGTGCATT 
GGAAACACAG ACGACACCCT CGGTTTTGCA CTGGGAGCTC TTTTCGTCAA AGCCACCTTC 
GACAAACAGA GCAAAGAAAT AGACCTCAAC GGTGGTGCGT TTTTGCCCTC ATGTGGCCGT 
AATGTGAACT CCACAGTTCA CAACCTCATG TTTTCTGTCA TAACGCAGGC AGAGGGGATG 
ATCAATGAAA TCCGCACGGC TTTCAAAGGC GCGCTGGATG ATTTGAAGTG GATGGATGAA 
CAGACCCGAC AAGCTGCCAA GGACAAGGCG GATGCCATCT ATGACATGAT CGGATTTCCA 
GACTTCATTC TGGACTCCAA AGAGCTGGAT GATGTGTATG ATGGGTATGA GGTTACGGAG 
GACAACTTCT TCCAGAACAT GATTAACTTC TATAACTTCT CAGCACGAGT GATGGCAGAT 
CAGCTCCGCA AGCCACCAAA CAGAGACCAA TGGAGTATGA CTCCACCGAC AGTGAACGCT 
TACTACATGC CAACCAAGAA CGGAATCGTC TTTCCCGCTG GCATCTTACA GGCCCCGTTC 
TACGCCCAGG ATCACCCCAA AGCTTTAAAC TTTGGTGGGA TTGGAGTGGT CATGGGACAT 
GAGCTAACGC ATGCGTTTGA TGACCAAGGC CGAGAGTACG ACAAAGATGG AAACCTGCGC 
TCGTGGTGGC AGAACTCCTC GGTGGAGGCC TTCAAGAACC GCACCGAGTG CATGGTCGAT 
CAATACACAC AGTACACCAT CAACGGAGAG CACATTAATG GAAAACAGAC GCTGGGAGAA 
AACATCGCAG ACAATGGCGG CCTGAAAGCT GCCTATCATG CGTACAGGTC CTGGGTGCAG 
AAGAATGGGG AAGAGAAGAG ACTCCCAGCT GTCAATCTGA CAAACGACCA GCTCTTCTTT 
GTGGGTTTTG CACAGGTGTG GTGCTCTGTC CGGACGCCAG AAAGCGCTCA CGAGGGCCTG 
ATGACGGATC CTCACAGTCC GCCCAAATAC CGCGTGATCG GCACCCTGTC TAACTCACCC 
GAGTTCGCCG AGCACTTCCA ATGTCCGCTG GGCTCCTCCA TGAACTCAGG CCACCGCTGC 
GAGGTTTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206533.1
CDS	58..2409
Translation	MSNYKRATFEEEDGTDVPVDGAISPDSVEVGFRKGGIQLFGPLGRRTQLEVVLAGLLLASLLALFGCAVTLGVRYNRDPARSICLTEACVTVASKIVEALDRSADPCQDFYQYACGGWVRKNPLPDGRSRWSTFNSIWDQNQAVLKHLLENGTFNSSSEAERKTQSYYLSCLNEQRIEELGAQPLMDLITKIGGWNITESWDKENFLDVLKIISGPYRAQPFFTVSVSVDPKNSNSNVIQVDQSGLFLPSRDYYLNKTNEKVLKAYLDYMVELGLLLGGDKNSTRGQMQQILDFETALANITVPQDERRDEEKIYHKITIADLQVLAPAIEWLDYLNSVLSPLELNDTEPVVVYAKEYMQQVSELINKTDHSLLNNYMIWNLVQKGASSLDQRFENAQDKLLESLYGTKKSCTPRWQTCIGNTDDTLGFALGALFVKATFDKQSKEIDLNGGAFLPSCGRNVNSTVHNLMFSVITQAEGMINEIRTAFKGALDDLKWMDEQTRQAAKDKADAIYDMIGFPDFILDSKELDDVYDGYEVTEDNFFQNMINFYNFSARVMADQLRKPPNRDQWSMTPPTVNAYYMPTKNGIVFPAGILQAPFYAQDHPKALNFGGIGVVMGHELTHAFDDQGREYDKDGNLRSWWQNSSVEAFKNRTECMVDQYTQYTINGEHINGKQTLGENIADNGGLKAAYHAYRSWVQKNGEEKRLPAVNLTNDQLFFVGFAQVWCSVRTPESAHEGLMTDPHSPPKYRVIGTLSNSPEFAEHFQCPLGSSMNSGHRCEVW

Target ORF information:

RefSeq Version	XM_017351044.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio endothelin converting enzyme 2b (ece2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351044.2

ORF Insert Sequence:

ATGTCTAACT ACAAGCGTGC AACGTTTGAG GAGGAGGACG GGACCGATGT CCCTGTTGAT 
GGAGCCATTT CTCCTGACAG TGTTGAGGTG GGTTTCCGTA AGGGTGGTAT TCAGTTGTTT 
GGTCCACTGG GCCGGAGGAC TCAGCTGGAG GTAGTGTTGG CCGGTCTGCT TCTTGCTTCA 
CTTCTTGCAC TGTTCGGCTG TGCGGTCACA CTGGGAGTCC GTTATAACAG AGATCCAGCC 
CGTAGTATCT GTCTAACCGA GGCCTGTGTC ACTGTGGCCA GCAAGATTGT TGAAGCTTTG 
GACCGCTCTG CCGACCCATG TCAGGATTTT TACCAATACG CCTGTGGAGG ATGGGTCCGC 
AAAAACCCCC TGCCAGATGG CCGGTCCCGC TGGAGCACAT TTAACAGCAT CTGGGACCAG 
AACCAGGCTG TGCTCAAACA CCTGCTGGAA AATGGCACTT TTAACTCCAG CAGTGAAGCA 
GAGAGGAAGA CACAGTCGTA CTACCTTTCC TGTCTTAATG AACAGCGAAT AGAAGAACTC 
GGAGCCCAAC CACTCATGGA TTTGATTACA AAGATCGGAG GCTGGAACAT CACAGAGTCC 
TGGGACAAAG AGAATTTTCT GGATGTTTTA AAGATCATTT CTGGACCATA CAGAGCTCAG 
CCCTTCTTCA CCGTCAGTGT TAGCGTAGAC CCCAAAAACT CCAACAGCAA TGTGATTCAG 
GTCGACCAAT CAGGACTCTT CCTCCCGTCC CGAGATTATT ACCTCAATAA GACTAACGAG 
AAGGTTTTGA AAGCTTATCT GGATTACATG GTGGAGCTGG GTTTGTTGTT GGGAGGAGAC 
AAGAATTCCA CTCGGGGTCA GATGCAGCAG ATTTTGGACT TTGAGACGGC GCTGGCGAAC 
ATCACCGTGC CCCAGGACGA ACGGCGAGAC GAGGAGAAGA TCTACCACAA GATAACCATA 
GCTGACCTCC AGGTCTTGGC TCCGGCTATT GAATGGTTGG ATTATCTGAA CTCAGTACTT 
TCACCGCTGG AGCTGAACGA CACAGAACCG GTGGTGGTGT ATGCTAAAGA GTACATGCAA 
CAAGTTTCAG AGCTCATCAA CAAGACCGAC CACAGTCTTC TGAATAATTA CATGATCTGG 
AATCTGGTGC AGAAAGGAGC GTCCAGCCTT GATCAGCGCT TTGAAAATGC TCAGGATAAA 
CTGCTGGAGA GCCTCTATGG CACCAAAAAG TCTTGTACTC CGCGCTGGCA GACGTGCATT 
GGAAACACAG ACGACACCCT CGGTTTTGCA CTGGGAGCTC TTTTCGTCAA AGCCACCTTC 
GACAAACAGA GCAAAGAAAT AGACCTCAAC GGTGGTGCGT TTTTGCCCTC ATGTGGCCGT 
AATGTGAACT CCACAGTTCA CAACCTCATG TTTTCTGTCA TAACGCAGGC AGAGGGGATG 
ATCAATGAAA TCCGCACGGC TTTCAAAGGC GCGCTGGATG ATTTGAAGTG GATGGATGAA 
CAGACCCGAC AAGCTGCCAA GGACAAGGCG GATGCCATCT ATGACATGAT CGGATTTCCA 
GACTTCATTC TGGACTCCAA AGAGCTGGAT GATGTGTATG ATGGGTATGA GGTTACGGAG 
GACAACTTCT TCCAGAACAT GATTAACTTC TATAACTTCT CAGCACGAGT GATGGCAGAT 
CAGCTCCGCA AGCCACCAAA CAGAGACCAA TGGAGTATGA CTCCACCGAC AGTGAACGCT 
TACTACATGC CAACCAAGAA CGGAATCGTC TTTCCCGCTG GCATCTTACA GGCCCCGTTC 
TACGCCCAGG ATCACCCCAA AGCTTTAAAC TTTGGTGGGA TTGGAGTGGT CATGGGACAT 
GAGCTAACGC ATGCGTTTGA TGACCAAGGC CGAGAGTACG ACAAAGATGG AAACCTGCGC 
TCGTGGTGGC AGAACTCCTC GGTGGAGGCC TTCAAGAACC GCACCGAGTG CATGGTCGAT 
CAATACACAC AGTACACCAT CAACGGAGAG CACATTAATG GAAAACAGAC GCTGGGAGAA 
AACATCGCAG ACAATGGCGG CCTGAAAGCT GCCTATCATG CGTACAGGTC CTGGGTGCAG 
AAGAATGGGG AAGAGAAGAG ACTCCCAGCT GTCAATCTGA CAAACGACCA GCTCTTCTTT 
GTGGGTTTTG CACAGGTGTG GTGCTCTGTC CGGACGCCAG AAAGCGCTCA CGAGGGCCTG 
ATGACGGATC CTCACAGTCC GCCCAAATAC CGCGTGATCG GCACCCTGTC TAACTCACCC 
GAGTTCGCCG AGCACTTCCA ATGTCCGCTG GGCTCCTCCA TGAACTCAGG CCACCGCTGC 
GAGGTTTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The construction of intrahepatic cholangiocarcinoma model in zebrafish.
Scientific reports7(1)13419(2017 Oct)
Wang J,Leng X,Wang G,Wan X,Cao H

Microenvironment-derived factors driving metastatic plasticity in melanoma.
Nature communications814343(2017 Feb)
Kim IS,Heilmann S,Kansler ER,Zhang Y,Zimmer M,Ratnakumar K,Bowman RL,Simon-Vermot T,Fennell M,Garippa R,Lu L,Lee W,Hollmann T,Xavier JB,White RM

Endothelin signalling in iridophore development and stripe pattern formation of zebrafish.
Biology open3(6)503-9(2014 May)
Krauss J,Frohnh?fer HG,Walderich B,Maischein HM,Weiler C,Irion U,N?sslein-Volhard C

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ECE2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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