selenou1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenou1b
Entrez Gene ID	767744
Full Name	selenoprotein U1b
Synonyms	fam213ab,selu1b,zgc:153444
General protein information	Preferred Names selenoprotein U1b Names selenoprotein U 1b family with sequence similarity 213, member Ab
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to a subfamily of FAM213A/selenoprotein U (SelU), within a peroxiredoxin-like FAM213 superfamily. SelU is unusual in that it has restricted phylogenetic distribution in vertebrates, such as in chicken and fish. Other vertebrate members of this family, including mammals, contain cysteine-containing homologues. SelU proteins contain UxxC (U for selenocysteine and C for cysteine) motif in place of the catalytic CxxC motif found in cysteine-containing homologues. The latter function as redox regulatory proteins, suggesting a similar role for SelU proteins. Like majority of selenoproteins, SelU contains a single selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs in vertebrates contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A paralog of this gene exists on chromosome 13 (GeneID:323739). [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076713.1	NP_001070181.1	uncharacterized protein LOC767744
NM_001076713.2	NP_001070181.2	selenoprotein U 1b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Reconsidering the evolution of eukaryotic selenoproteins: a novel nonmammalian family with scattered phylogenetic distribution.
Castellano S, Novoselov SV, Kryukov GV, Lescure A, Blanco E, Krol A, Gladyshev VN, Guig? R
EMBO reports5(1)71-7(2004 Jan)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenou1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenou1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04886	NM_001076713.1 Latest version!	Danio rerio family with sequence similarity 213, member Ab (fam213ab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa141173	NM_001076713.2 Latest version!	Danio rerio selenoprotein U1b (selenou1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenou1b ( NM_001076713.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070181.1 Danio rerio family with sequence similarity 213, member Ab (fam213ab), mRNA.

Price & Availability↑

CloneID	ODa04886
Clone ID Related Accession (Same CDS sequence)	NM_001076713.1
Accession Version	NM_001076713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-17
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC767744
Comment	PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC124355.1. ##Evidence-Data-START## Transcript exon combination :: BC124355.1 [ECO:0000332] ##Evidence-Data-END##

ATGTGCAGAG AGGAGGCGTC CGATCTCTCC TCTCTGAAGG CTCAGTTGGA TGAGTGTGGA 
GTGTATCTGT ATGCTGTGGT CAAAGAGAAC GTCGGCTCAG AGATTCAGCT CTTCAGGCCG 
TATTTCAGCG GGGAGATTTT CCTGGATCAG CAGAGGGGTT TCTTCGGGCC GCTGGAGCGC 
TGGATGGGGG TCTCTGGGAT TCTGCGTGGC GGAGTCTGGA TGAACGGATG GAGGGCGTAT 
CAGAGCGGAT ACTGGGGAAA CGTCAAGGGC GAAGGCTTTG TTTTGGGGGC CGTTTATGTC 
ATCGGACCAC AACAACAGGG AATCCTCCTG GAGCACAGAG AGATGGAGTT TGGGGATAAA 
GTCCAGAAGT CTGATGTGCT TCAAGCTGTT GAACAGATCA AAACTCAACA ATTATCTCTG 
AAATTCAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070181.1
CDS	243..674
Translation	MCREEASDLSSLKAQLDECGVYLYAVVKENVGSEIQLFRPYFSGEIFLDQQRGFFGPLERWMGVSGILRGGVWMNGWRAYQSGYWGNVKGEGFVLGAVYVIGPQQQGILLEHREMEFGDKVQKSDVLQAVEQIKTQQLSLKFK

Target ORF information:

RefSeq Version	NM_001076713.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 213, member Ab (fam213ab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076713.1

ORF Insert Sequence:

ATGTGCAGAG AGGAGGCGTC CGATCTCTCC TCTCTGAAGG CTCAGTTGGA TGAGTGTGGA 
GTGTATCTGT ATGCTGTGGT CAAAGAGAAC GTCGGCTCAG AGATTCAGCT CTTCAGGCCG 
TATTTCAGCG GGGAGATTTT CCTGGATCAG CAGAGGGGTT TCTTCGGGCC GCTGGAGCGC 
TGGATGGGGG TCTCTGGGAT TCTGCGTGGC GGAGTCTGGA TGAACGGATG GAGGGCGTAT 
CAGAGCGGAT ACTGGGGAAA CGTCAAGGGC GAAGGCTTTG TTTTGGGGGC CGTTTATGTC 
ATCGGACCAC AACAACAGGG AATCCTCCTG GAGCACAGAG AGATGGAGTT TGGGGATAAA 
GTCCAGAAGT CTGATGTGCT TCAAGCTGTT GAACAGATCA AAACTCAACA ATTATCTCTG 
AAATTCAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

selenou1b ( NM_001076713.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070181.2 Danio rerio selenoprotein U1b (selenou1b), mRNA.

Price & Availability↑

CloneID	ODa141173
Clone ID Related Accession (Same CDS sequence)	NM_001076713.2
Accession Version	NM_001076713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-25
Organism	Danio rerio(zebrafish)
Product	selenoprotein U 1b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CABZ01088095.1 and LO017725.3. On Aug 14, 2017 this sequence version replaced NM_001076713.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC124355.1, EH433041.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG TGACCCGTGC CGTCGGTTCT GTGGGCTTGA CTGTCATCGA GGCGTTACGA 
TCTTTCACTG AGCTCTTTCT CACACAGCCC GTGTGTGCAA CGCTCACTCA ACTGGCCGAC 
ACTGACCTCA GAACACTGGA CGGAGATGAT CGAGTGTTTA AGGCCAGAGA ACTGTGGGAG 
AGTTCAGGAG CTGTTATTAT GGCTGTGAGG AGACCTGGAT GATTCATGTG CAGAGAGGAG 
GTGTCTGATC TCTCCTCTCT GAAGGCTCAG TTGGATGAGT GTGGAGTGTG TCTGTATGCG 
GTGGTCAAAG AGAACGTCGG CTCAGAGATT CAGCTCTTCA GGCTGTATTT CAGCGGGGAG 
ATTTTCCTGG ATCAGCAGAG GGGTTTCTTC GGGCCGCTGG AGCGCTGGAT GGGGGTCTCT 
GGGATTCTGC GTGGCGGAGT CTGGATGAAC GGATGGAGGG CGTATCAGAG CGGATACTGG 
GGAAACGTCA AGGGCGAAGG CTTTGTTTTG GGGGCCGTTT ATGTCATCGG ACCACAACAA 
CAGGGAATCC TCCTGGAGCA CAGAGAGATG GAGTTTGGGG ATAAAGTCCA GAAGTCTGAT 
GTGCTTCAAG CTGTTGAACA GATCAAAACT CAACAATTAT CTCTGAAATT CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070181.2
CDS	55..711
Translation	MELVTRAVGSVGLTVIEALRSFTELFLTQPVCATLTQLADTDLRTLDGDDRVFKARELWESSGAVIMAVRRPG*FMCREEVSDLSSLKAQLDECGVCLYAVVKENVGSEIQLFRLYFSGEIFLDQQRGFFGPLERWMGVSGILRGGVWMNGWRAYQSGYWGNVKGEGFVLGAVYVIGPQQQGILLEHREMEFGDKVQKSDVLQAVEQIKTQQLSLKFK

Target ORF information:

RefSeq Version	NM_001076713.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein U1b (selenou1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076713.2

ORF Insert Sequence:

ATGGAGCTGG TGACCCGTGC CGTCGGTTCT GTGGGCTTGA CTGTCATCGA GGCGTTACGA 
TCTTTCACTG AGCTCTTTCT CACACAGCCC GTGTGTGCAA CGCTCACTCA ACTGGCCGAC 
ACTGACCTCA GAACACTGGA CGGAGATGAT CGAGTGTTTA AGGCCAGAGA ACTGTGGGAG 
AGTTCAGGAG CTGTTATTAT GGCTGTGAGG AGACCTGGAT GATTCATGTG CAGAGAGGAG 
GTGTCTGATC TCTCCTCTCT GAAGGCTCAG TTGGATGAGT GTGGAGTGTG TCTGTATGCG 
GTGGTCAAAG AGAACGTCGG CTCAGAGATT CAGCTCTTCA GGCTGTATTT CAGCGGGGAG 
ATTTTCCTGG ATCAGCAGAG GGGTTTCTTC GGGCCGCTGG AGCGCTGGAT GGGGGTCTCT 
GGGATTCTGC GTGGCGGAGT CTGGATGAAC GGATGGAGGG CGTATCAGAG CGGATACTGG 
GGAAACGTCA AGGGCGAAGG CTTTGTTTTG GGGGCCGTTT ATGTCATCGG ACCACAACAA 
CAGGGAATCC TCCTGGAGCA CAGAGAGATG GAGTTTGGGG ATAAAGTCCA GAAGTCTGAT 
GTGCTTCAAG CTGTTGAACA GATCAAAACT CAACAATTAT CTCTGAAATT CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Reconsidering the evolution of eukaryotic selenoproteins: a novel nonmammalian family with scattered phylogenetic distribution.
EMBO reports5(1)71-7(2004 Jan)
Castellano S,Novoselov SV,Kryukov GV,Lescure A,Blanco E,Krol A,Gladyshev VN,Guig? R

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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selenou1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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