rfx3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rfx3
Entrez Gene ID	570989
Full Name	regulatory factor X, 3 (influences HLA class II expression)
Synonyms	zgc:153245
General protein information	Preferred Names regulatory factor X, 3 (influences HLA class II expression) Names transcription factor RFX3 regulatory factor X 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001080048.1	NP_001073517.1	transcription factor RFX3
XM_021479192.1	XP_021334867.1	transcription factor RFX3 isoform X1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	daf-19	NP_001254236.1
Pan troglodytes (chimpanzee)	RFX3	XP_520464.2
Homo sapiens (human)	RFX3	NP_001269045.1
	RFX3	XP_001086729.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003943	XP_318401.5
Canis lupus familiaris (dog)	RFX3	XP_005615977.1
Mus musculus (house mouse)	Rfx3	NP_001159886.1
Gallus gallus (chicken)	RFX3	XP_004949116.1
Danio rerio (zebrafish)	rfx3	NP_001073517.1
Bos taurus (cattle)	LOC538070	XP_005210053.1
Drosophila melanogaster (fruit fly)	Rfx	NP_001247022.2
Xenopus tropicalis (tropical clawed frog)	rfx3	NP_001263620.1

Gene Ontology
Bibliography

Related articles in PubMed

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9.
Feng Y, Chen C, Han Y, Chen Z, Lu X, Liang F, Li S, Qin W, Lin S
G3 (Bethesda, Md.)6(8)2517-21(2016 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development.
Soyer J, Flasse L, Raffelsberger W, Beucher A, Orvain C, Peers B, Ravassard P, Vermot J, Voz ML, Mellitzer G, Gradwohl G
Development (Cambridge, England)137(2)203-12(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rfx3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rfx3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38754	NM_001080048.1 Latest version!	Danio rerio regulatory factor X, 3 (influences HLA class II expression) (rfx3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa147627	XM_021479192.1 Latest version!	Danio rerio regulatory factor X, 3 (influences HLA class II expression) (rfx3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rfx3 ( NM_001080048.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073517.1 Danio rerio regulatory factor X, 3 (influences HLA class II expression) (rfx3), mRNA.

Price & Availability↑

CloneID	ODa38754
Clone ID Related Accession (Same CDS sequence)	NM_001080048.1
Accession Version	NM_001080048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	transcription factor RFX3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125862.1. On Mar 11, 2007 this sequence version replaced XM_694531.2. ##Evidence-Data-START## CDS exon combination :: BC125862.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGACCC CAGAGGCAGG CGCTGACTCC ACCGTGTCCA CCGTGCCCCT CCAGACCAGC 
GTGCCTGTGC AGCCTGCGGT CAGTGGCCAG CAGGTGGCGT CTCAGGTGCC CGTCCAGCAG 
CAGGCGCAGA CTGTTCAACA GGTCCAGCAT GTGTATCAAG CACAGGTTCA GTATGTGCAG 
GAGGAAAACA ACAGCGTTTA CACCAACGGA ACCATACGGG CGTACTCGTA CTCGGAGCCA 
CAGCTCTACA ACCAGAACAG CAGCGGGAAC TACTTCGACA CGCAGGGCGG CGGAGGCAGC 
GCGGCACAGG TCAGCACCGT AGTGTCCACT CACAGCATGG CCAACAACGG CAACGGCACC 
GGCGCTCTGG CTATGGGGCT CACCGGAGGT CAGATCATCA GCAGCTCAGG AGCTTATCTG 
ATTGGAGGAA ACTCCATGGA TGGCTCCGCC CCTCACGGCG CCGCACAGAC CACCAGAGCC 
TCCCCTGCTA CGATTGAAAT GGCGATTGAG ACGCTCCAAA AGTCTGAAGG TTTATCCAGT 
CAGAGAAGTT CGCTGCTCAA CAGCCATCTG CAGTGGCTGC TGGATAATTA CGAGACAGCA 
GAGGGCGTGA GTCTGCCGCG CTCCACCCTC TACAATCACT ACCTGCGCCA CTGTCAGGAG 
CAGAAGCTGG ACCCTGTCAA TGCTGCGTCC TTCGGGAAGC TCATCCGCTC CATCTTCATG 
GGGCTGCGCA CACGCCGCCT GGGGACACGA GGGAACTCAA AGTATCATTA TTACGGGATT 
CGTGTGAAGC CGGACTCTCC TCTGAACCGC TTGCAGGAGG ACATGCAGTA CATGGCACTG 
AGACAGCAGC CCGTCCAGCA GAAGCAGAGA TTTAAGCCTG TGCAGAAGAT GGACGGAGTT 
TCGGGAGATA ACTTCAGCAG CGCTGGACAG CACACACCCA GTGCTGCAGA GCAGACCTTC 
ATCGCTCAGA GCCAACATCA TCAGCAGTTC CTGGATGGCT CCAGAGCTCT GCCGGAGTTT 
GTTGAACTGG ATCTTGGAGA GGCAGTGGAC GGCGTCGGCC CAGAGGATGT CAAAGCCCTT 
CAGACGCTCT ACAGAGAACA CTGCGAGGCC ATCCTGGATG TAGTGGTGAA CCTGCAGTTC 
AATCTGATCG AGAACCTGTG GCAGACGTTC TGGCGCTATT CTGCCTCCAG CTCTGTAGAG 
GGCGTCACAA TCACAGAAAA CAGTGGTTTG AGTGAGATCG AGGGCCGTCT GCCGCGGGCC 
AGGCTGATTC TGCTGTGCCG ACATGAGGCC GTGCACAAGT GGATGAACAC CTGCGATCAC 
CTGATGTACC AGGCCCTCGT GGAGATCCTC ATCCCGGACG TCCTGAGACC CATTCCTAGT 
GCCTTGACTC AAGCCATCCG TAACTTTGCC AAAAGCCTTG AGGGCTGGCT CACCAATGCC 
ATGAGCAGCA TTCCCCCGAG GATGATCAAT ACCAAGGTTT CCGCTGTTAG TGCCTTCGCT 
CAGACCCTCC GCAGATACAC GTCCCTGAAT CATCTGGCGC AGGCGGCTCG CGCAGTCCTG 
CAGAACACCT CGCAGATCAA CCAGATGCTC AGCGATCTCA ACCGGGTCGA CTTCGCCAAT 
GTGCAGGAGC AGGCCTCATG GGTGTGCCAG TGTGAAGAGG GTGTCGTCCA GCGCCTCGAG 
CAGGATTTTA AGGCCACCCT GCAGCAGCAG AGCTCACTGG AGCAGTGGGC CGCCTGGCTG 
GATAACGTGG TCACCCAGAT ACTGAAGCCG TACGAGGACA AGCCCACCCT GCCCAAAGCT 
GCACGCCAGT TCCTGCTCAA GTGGTCCTTC TACAGCTCTA TGGTCATCAG AGATCTGACC 
CTCCGCAGCG CTGCCAGCTT CGGCTCGTTC CACCTGATCC GGCTGCTGTA CGACGAGTAC 
ATGTTCTACC TGGTGGAGCA TCGCGTCGCC CAGGCAACAG GAGAGACGGC CATCGGAGTC 
ATGGGCGAGT TTCAGGACCT GAACACCATG TCACCTGCCA ACATCGACAA AGATGAGGTG 
AGCGAGATGG ACAGCGATCT GGACGAGGAG ATGGAGGAGG CGGACGAGCC ACTGGCCAAG 
CGGGAGAAGG TGGAGGCGGA CGTGATCCAG GTGCTGCAGG TAGGGGCGAT GGAGGATGGC 
AGCAGCGCGG TGGTGGGCAT CGTCCAGCCG AGCATGATCA ACTCTCTGCC CCCGACCACC 
AACAATCACA GCGAACACAT CCTCAGCACC GGAACCAGCA CTATCCGCCA TGTGGGAAAC 
ACCTACGCCT CCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073517.1
CDS	197..2494
Translation	MQTPEAGADSTVSTVPLQTSVPVQPAVSGQQVASQVPVQQQAQTVQQVQHVYQAQVQYVQEENNSVYTNGTIRAYSYSEPQLYNQNSSGNYFDTQGGGGSAAQVSTVVSTHSMANNGNGTGALAMGLTGGQIISSSGAYLIGGNSMDGSAPHGAAQTTRASPATIEMAIETLQKSEGLSSQRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEQKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMALRQQPVQQKQRFKPVQKMDGVSGDNFSSAGQHTPSAAEQTFIAQSQHHQQFLDGSRALPEFVELDLGEAVDGVGPEDVKALQTLYREHCEAILDVVVNLQFNLIENLWQTFWRYSASSSVEGVTITENSGLSEIEGRLPRARLILLCRHEAVHKWMNTCDHLMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLTNAMSSIPPRMINTKVSAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEEGVVQRLEQDFKATLQQQSSLEQWAAWLDNVVTQILKPYEDKPTLPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETAIGVMGEFQDLNTMSPANIDKDEVSEMDSDLDEEMEEADEPLAKREKVEADVIQVLQVGAMEDGSSAVVGIVQPSMINSLPPTTNNHSEHILSTGTSTIRHVGNTYASV

Target ORF information:

RefSeq Version	NM_001080048.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulatory factor X, 3 (influences HLA class II expression) (rfx3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080048.1

ORF Insert Sequence:

ATGCAGACCC CAGAGGCAGG CGCTGACTCC ACCGTGTCCA CCGTGCCCCT CCAGACCAGC 
GTGCCTGTGC AGCCTGCGGT CAGTGGCCAG CAGGTGGCGT CTCAGGTGCC CGTCCAGCAG 
CAGGCGCAGA CTGTTCAACA GGTCCAGCAT GTGTATCAAG CACAGGTTCA GTATGTGCAG 
GAGGAAAACA ACAGCGTTTA CACCAACGGA ACCATACGGG CGTACTCGTA CTCGGAGCCA 
CAGCTCTACA ACCAGAACAG CAGCGGGAAC TACTTCGACA CGCAGGGCGG CGGAGGCAGC 
GCGGCACAGG TCAGCACCGT AGTGTCCACT CACAGCATGG CCAACAACGG CAACGGCACC 
GGCGCTCTGG CTATGGGGCT CACCGGAGGT CAGATCATCA GCAGCTCAGG AGCTTATCTG 
ATTGGAGGAA ACTCCATGGA TGGCTCCGCC CCTCACGGCG CCGCACAGAC CACCAGAGCC 
TCCCCTGCTA CGATTGAAAT GGCGATTGAG ACGCTCCAAA AGTCTGAAGG TTTATCCAGT 
CAGAGAAGTT CGCTGCTCAA CAGCCATCTG CAGTGGCTGC TGGATAATTA CGAGACAGCA 
GAGGGCGTGA GTCTGCCGCG CTCCACCCTC TACAATCACT ACCTGCGCCA CTGTCAGGAG 
CAGAAGCTGG ACCCTGTCAA TGCTGCGTCC TTCGGGAAGC TCATCCGCTC CATCTTCATG 
GGGCTGCGCA CACGCCGCCT GGGGACACGA GGGAACTCAA AGTATCATTA TTACGGGATT 
CGTGTGAAGC CGGACTCTCC TCTGAACCGC TTGCAGGAGG ACATGCAGTA CATGGCACTG 
AGACAGCAGC CCGTCCAGCA GAAGCAGAGA TTTAAGCCTG TGCAGAAGAT GGACGGAGTT 
TCGGGAGATA ACTTCAGCAG CGCTGGACAG CACACACCCA GTGCTGCAGA GCAGACCTTC 
ATCGCTCAGA GCCAACATCA TCAGCAGTTC CTGGATGGCT CCAGAGCTCT GCCGGAGTTT 
GTTGAACTGG ATCTTGGAGA GGCAGTGGAC GGCGTCGGCC CAGAGGATGT CAAAGCCCTT 
CAGACGCTCT ACAGAGAACA CTGCGAGGCC ATCCTGGATG TAGTGGTGAA CCTGCAGTTC 
AATCTGATCG AGAACCTGTG GCAGACGTTC TGGCGCTATT CTGCCTCCAG CTCTGTAGAG 
GGCGTCACAA TCACAGAAAA CAGTGGTTTG AGTGAGATCG AGGGCCGTCT GCCGCGGGCC 
AGGCTGATTC TGCTGTGCCG ACATGAGGCC GTGCACAAGT GGATGAACAC CTGCGATCAC 
CTGATGTACC AGGCCCTCGT GGAGATCCTC ATCCCGGACG TCCTGAGACC CATTCCTAGT 
GCCTTGACTC AAGCCATCCG TAACTTTGCC AAAAGCCTTG AGGGCTGGCT CACCAATGCC 
ATGAGCAGCA TTCCCCCGAG GATGATCAAT ACCAAGGTTT CCGCTGTTAG TGCCTTCGCT 
CAGACCCTCC GCAGATACAC GTCCCTGAAT CATCTGGCGC AGGCGGCTCG CGCAGTCCTG 
CAGAACACCT CGCAGATCAA CCAGATGCTC AGCGATCTCA ACCGGGTCGA CTTCGCCAAT 
GTGCAGGAGC AGGCCTCATG GGTGTGCCAG TGTGAAGAGG GTGTCGTCCA GCGCCTCGAG 
CAGGATTTTA AGGCCACCCT GCAGCAGCAG AGCTCACTGG AGCAGTGGGC CGCCTGGCTG 
GATAACGTGG TCACCCAGAT ACTGAAGCCG TACGAGGACA AGCCCACCCT GCCCAAAGCT 
GCACGCCAGT TCCTGCTCAA GTGGTCCTTC TACAGCTCTA TGGTCATCAG AGATCTGACC 
CTCCGCAGCG CTGCCAGCTT CGGCTCGTTC CACCTGATCC GGCTGCTGTA CGACGAGTAC 
ATGTTCTACC TGGTGGAGCA TCGCGTCGCC CAGGCAACAG GAGAGACGGC CATCGGAGTC 
ATGGGCGAGT TTCAGGACCT GAACACCATG TCACCTGCCA ACATCGACAA AGATGAGGTG 
AGCGAGATGG ACAGCGATCT GGACGAGGAG ATGGAGGAGG CGGACGAGCC ACTGGCCAAG 
CGGGAGAAGG TGGAGGCGGA CGTGATCCAG GTGCTGCAGG TAGGGGCGAT GGAGGATGGC 
AGCAGCGCGG TGGTGGGCAT CGTCCAGCCG AGCATGATCA ACTCTCTGCC CCCGACCACC 
AACAATCACA GCGAACACAT CCTCAGCACC GGAACCAGCA CTATCCGCCA TGTGGGAAAC 
ACCTACGCCT CCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rfx3 ( XM_021479192.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334867.1 Danio rerio regulatory factor X, 3 (influences HLA class II expression) (rfx3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147627
Clone ID Related Accession (Same CDS sequence)	XM_021479192.1
Accession Version	XM_021479192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transcription factor RFX3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CAGAGGCAGG CGCTGACTCC ACCGTGTCCA CCGTGCCCCT CCAGACCAGC 
GTGCCTGTGC AGCCTGCGGT CAGTGGCCAG CAGGTGGCGT CTCAGGTGCC CGTCCAGCAG 
CAGGCGCAGA CTGTTCAACA GGTCCAGCAT GTGTATCAAG CACAGGTTCA GTATGTGCAG 
GAGGAAAACA ACAGCGTTTA CACCAACGGA ACCATACGGG CGTACTCGTA CTCGGAGCCA 
CAGCTCTACA ACCAGAACAG CAGCGGGAAC TACTTCGACA CGCAGGGCGG CGGAGGCAGC 
GCGGCACAGG TCAGCACCGT AGTGTCCACT CACAGCATGG CCAACAACGG CAACGGCACC 
GGCGCTCTGG CTATGGGGCT CACCGGAGGC CAGATCATCA GCAGCTCAGG AGCTTATCTG 
ATTGGAGGAA ACTCCATGGA TGGCTCCGCC CCTCACGGCG CCGCACAGAC CACCAGAGCC 
TCCCCTGCTA CGCTGCAGTG GCTGCTGGAT AATTACGAGA CAGCAGAGGG CGTGAGTCTG 
CCGCGCTCCA CCCTCTACAA TCACTACCTG CGCCACTGTC AGGAGCAGAA GCTGGACCCT 
GTCAATGCTG CGTCCTTCGG GAAGCTCATC CGCTCCATCT TCATGGGGCT GCGCACACGC 
CGCCTGGGGA CACGAGGGAA CTCAAAGTAT CATTATTACG GGATTCGTGT GAAGCCGGAC 
TCTCCTCTGA ACCGCTTGCA GGAGGACATG CAGTACATGG CACTGAGACA GCAGCCCGTC 
CAGCAGAAGC AGAGATTTAA GCCTGTGCAG AAGATGGACG GAGTTTCGGG AGATAACTTC 
AGCAGCGCTG GACAGCACAC ACCCAATGCT GCAGAGCAGA CCTTCATCGC TCAGAGCCAA 
CATCATCAGC AGTTCCTGGA TGGCTCCAGA GCTCTGCCGG AGTTTGTTGA ACTGGATCTT 
GGAGAGGCAG TGGACGGCGT CGGCCCAGAG GATGTCAAAG CCCTTCAGAC GCTCTACAGA 
GAACACTGCG AGGCCATCCT GGATGTAGTG GTGAACCTGC AGTTCAATCT GATCGAGAAC 
CTGTGGCAGA CGTTCTGGCG CTATTCTGCC TCCAGCTCTG TAGAGGGCGT CACAATCACA 
GAAAACAGTG GTTTGAGTGA GATCGAGGGC CGTCTGCCGC GGGCCAGGCT GATTCTGCTG 
TGCCGACATG AGGCCGTGCA CAAGTGGATG AACACCTGCG ATCACCTGAT GTACCAGGCC 
CTCGTGGAGA TCCTCATCCC GGACGTCCTG AGACCCATTC CTAGTGCCTT GACTCAAGCC 
ATCCGTAACT TTGCCAAAAG CCTTGAGGGC TGGCTCACCA ATGCCATGAG CAGCATTCCC 
CCGAGGATGA TCAATACCAA GGTTTCCGCT GTTAGTGCCT TCGCTCAGAC CCTCCGCAGA 
TACACGTCCC TGAATCATCT GGCGCAGGCG GCTCGCGCAG TCCTGCAGAA CACCTCGCAG 
ATCAACCAGA TGCTCAGCGA TCTCAACCGG GTCGACTTCG CCAATGTGCA GGAGCAGGCC 
TCATGGGTGT GCCAGTGTGA AGAGGGTGTC GTCCAGCGCC TCGAGCAGGA TTTTAAGGCC 
ACCCTGCAGC AGCAGAGCTC ACTGGAGCAG TGGGCCGCCT GGCTGGATAA CGTGGTCACC 
CAGATACTGA AGCCGTACGA GGACAAGCCC ACCCTGCCCA AAGCTGCACG CCAGTTCCTG 
CTCAAGTGGT CCTTCTACAG CTCTATGGTC ATCAGAGATC TGACCCTCCG CAGCGCTGCC 
AGCTTCGGCT CGTTCCACCT GATCCGGCTG CTGTACGACG AGTACATGTT CTACCTGGTG 
GAGCATCGCG TCGCCCAGGC AACAGGAGAG ACGGCCATCG GAGTCATGGG CGAGTTTCAG 
GACCTGAACA CCATGTCACC TGCCAACATC GACAAAGATG AGGTGAGCGA GATGGACAGC 
GATCTGGACG AGGAGATGGA GGAGGCGGAC GAGCCACTGG CCAAGCGGGA GAAGGTGGAG 
GCGGACGTGA TCCAGGTGCT GCAGGTAGGG GCGATGGAGG ATGGCAGCAG CGCGGTGGTG 
GGCATCGTCC AGCCGAGCAT GATCAACTCT CTGCCCCCGA CCACCAACAA TCACAGCGAA 
CACATCCTCA GCACCGGAAC CAGCACTATC CGCCATGTGG GAAACACCTA CGCCTCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334867.1
CDS	214..2436
Translation	MQTPEAGADSTVSTVPLQTSVPVQPAVSGQQVASQVPVQQQAQTVQQVQHVYQAQVQYVQEENNSVYTNGTIRAYSYSEPQLYNQNSSGNYFDTQGGGGSAAQVSTVVSTHSMANNGNGTGALAMGLTGGQIISSSGAYLIGGNSMDGSAPHGAAQTTRASPATLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEQKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMALRQQPVQQKQRFKPVQKMDGVSGDNFSSAGQHTPNAAEQTFIAQSQHHQQFLDGSRALPEFVELDLGEAVDGVGPEDVKALQTLYREHCEAILDVVVNLQFNLIENLWQTFWRYSASSSVEGVTITENSGLSEIEGRLPRARLILLCRHEAVHKWMNTCDHLMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLTNAMSSIPPRMINTKVSAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEEGVVQRLEQDFKATLQQQSSLEQWAAWLDNVVTQILKPYEDKPTLPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETAIGVMGEFQDLNTMSPANIDKDEVSEMDSDLDEEMEEADEPLAKREKVEADVIQVLQVGAMEDGSSAVVGIVQPSMINSLPPTTNNHSEHILSTGTSTIRHVGNTYASV

Target ORF information:

RefSeq Version	XM_021479192.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulatory factor X, 3 (influences HLA class II expression) (rfx3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479192.1

ORF Insert Sequence:

ATGCAGACCC CAGAGGCAGG CGCTGACTCC ACCGTGTCCA CCGTGCCCCT CCAGACCAGC 
GTGCCTGTGC AGCCTGCGGT CAGTGGCCAG CAGGTGGCGT CTCAGGTGCC CGTCCAGCAG 
CAGGCGCAGA CTGTTCAACA GGTCCAGCAT GTGTATCAAG CACAGGTTCA GTATGTGCAG 
GAGGAAAACA ACAGCGTTTA CACCAACGGA ACCATACGGG CGTACTCGTA CTCGGAGCCA 
CAGCTCTACA ACCAGAACAG CAGCGGGAAC TACTTCGACA CGCAGGGCGG CGGAGGCAGC 
GCGGCACAGG TCAGCACCGT AGTGTCCACT CACAGCATGG CCAACAACGG CAACGGCACC 
GGCGCTCTGG CTATGGGGCT CACCGGAGGC CAGATCATCA GCAGCTCAGG AGCTTATCTG 
ATTGGAGGAA ACTCCATGGA TGGCTCCGCC CCTCACGGCG CCGCACAGAC CACCAGAGCC 
TCCCCTGCTA CGCTGCAGTG GCTGCTGGAT AATTACGAGA CAGCAGAGGG CGTGAGTCTG 
CCGCGCTCCA CCCTCTACAA TCACTACCTG CGCCACTGTC AGGAGCAGAA GCTGGACCCT 
GTCAATGCTG CGTCCTTCGG GAAGCTCATC CGCTCCATCT TCATGGGGCT GCGCACACGC 
CGCCTGGGGA CACGAGGGAA CTCAAAGTAT CATTATTACG GGATTCGTGT GAAGCCGGAC 
TCTCCTCTGA ACCGCTTGCA GGAGGACATG CAGTACATGG CACTGAGACA GCAGCCCGTC 
CAGCAGAAGC AGAGATTTAA GCCTGTGCAG AAGATGGACG GAGTTTCGGG AGATAACTTC 
AGCAGCGCTG GACAGCACAC ACCCAATGCT GCAGAGCAGA CCTTCATCGC TCAGAGCCAA 
CATCATCAGC AGTTCCTGGA TGGCTCCAGA GCTCTGCCGG AGTTTGTTGA ACTGGATCTT 
GGAGAGGCAG TGGACGGCGT CGGCCCAGAG GATGTCAAAG CCCTTCAGAC GCTCTACAGA 
GAACACTGCG AGGCCATCCT GGATGTAGTG GTGAACCTGC AGTTCAATCT GATCGAGAAC 
CTGTGGCAGA CGTTCTGGCG CTATTCTGCC TCCAGCTCTG TAGAGGGCGT CACAATCACA 
GAAAACAGTG GTTTGAGTGA GATCGAGGGC CGTCTGCCGC GGGCCAGGCT GATTCTGCTG 
TGCCGACATG AGGCCGTGCA CAAGTGGATG AACACCTGCG ATCACCTGAT GTACCAGGCC 
CTCGTGGAGA TCCTCATCCC GGACGTCCTG AGACCCATTC CTAGTGCCTT GACTCAAGCC 
ATCCGTAACT TTGCCAAAAG CCTTGAGGGC TGGCTCACCA ATGCCATGAG CAGCATTCCC 
CCGAGGATGA TCAATACCAA GGTTTCCGCT GTTAGTGCCT TCGCTCAGAC CCTCCGCAGA 
TACACGTCCC TGAATCATCT GGCGCAGGCG GCTCGCGCAG TCCTGCAGAA CACCTCGCAG 
ATCAACCAGA TGCTCAGCGA TCTCAACCGG GTCGACTTCG CCAATGTGCA GGAGCAGGCC 
TCATGGGTGT GCCAGTGTGA AGAGGGTGTC GTCCAGCGCC TCGAGCAGGA TTTTAAGGCC 
ACCCTGCAGC AGCAGAGCTC ACTGGAGCAG TGGGCCGCCT GGCTGGATAA CGTGGTCACC 
CAGATACTGA AGCCGTACGA GGACAAGCCC ACCCTGCCCA AAGCTGCACG CCAGTTCCTG 
CTCAAGTGGT CCTTCTACAG CTCTATGGTC ATCAGAGATC TGACCCTCCG CAGCGCTGCC 
AGCTTCGGCT CGTTCCACCT GATCCGGCTG CTGTACGACG AGTACATGTT CTACCTGGTG 
GAGCATCGCG TCGCCCAGGC AACAGGAGAG ACGGCCATCG GAGTCATGGG CGAGTTTCAG 
GACCTGAACA CCATGTCACC TGCCAACATC GACAAAGATG AGGTGAGCGA GATGGACAGC 
GATCTGGACG AGGAGATGGA GGAGGCGGAC GAGCCACTGG CCAAGCGGGA GAAGGTGGAG 
GCGGACGTGA TCCAGGTGCT GCAGGTAGGG GCGATGGAGG ATGGCAGCAG CGCGGTGGTG 
GGCATCGTCC AGCCGAGCAT GATCAACTCT CTGCCCCCGA CCACCAACAA TCACAGCGAA 
CACATCCTCA GCACCGGAAC CAGCACTATC CGCCATGTGG GAAACACCTA CGCCTCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9.
G3 (Bethesda, Md.)6(8)2517-21(2016 08)
Feng Y,Chen C,Han Y,Chen Z,Lu X,Liang F,Li S,Qin W,Lin S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development.
Development (Cambridge, England)137(2)203-12(2010 Jan)
Soyer J,Flasse L,Raffelsberger W,Beucher A,Orvain C,Peers B,Ravassard P,Vermot J,Voz ML,Mellitzer G,Gradwohl G

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rfx3 cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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