si:dkey-151g10.3 cDNA ORF clone, Danio rerio(zebrafish)

The following si:dkey-151g10.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-151g10.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146288 XM_009296929.3
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146284 XM_009296926.3
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146285 XM_690703.9
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146286 XM_009296927.3
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa146287 XM_009296928.3
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27775 XM_021469967.1
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27775 XM_690703.8
Latest version!
Danio rerio im:7152756 (im:7152756), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45152 XM_009296926.2
Latest version!
Danio rerio im:7152756 (im:7152756), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45152 XM_021469966.1
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45153 XM_009296927.2
Latest version!
Danio rerio im:7152756 (im:7152756), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45153 XM_021469968.1
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45154 XM_009296928.2
Latest version!
Danio rerio im:7152756 (im:7152756), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45154 XM_021469969.1
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa45155 XM_021469970.1
Latest version!
Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa45155 XM_009296929.2
Latest version!
Danio rerio im:7152756 (im:7152756), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146288
    Clone ID Related Accession (Same CDS sequence) XM_009296929.3
    Accession Version XM_009296929.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395152.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGGAAA GCCTAATTCA CTCTGCCAAC AGGGGATCTT TATTATGCTC TGCTCCTACC
    AATGGAGATG AGGGCCAGTT GGTGTGGCCT AGTGGAAAAC TAGATAAGGC AAAATGTCAG
    AGGAGATCAT CCTGTCAGAA GACTGAAAAA ACTCACTTCC AACTCTGCAT GTTGCAGGTC
    TCATGCACAG GAGACAACAT GGTAGAATGC CAGTTGGAGA CTCACAGCAA CAAGATGGTC
    ACGTTCAAGT TTGACACTGA GGGAGATGCA CCTGAAGACA TTGCAGATTA CATGGTGGAG
    GAGGACTTCG TCCTTGAGCC TGAAAAAGAA AAATTTGTTG AGGAGCTGAG ATCTATAGTG
    AAGAAAGCTC AAGCAATATT AAGTACTCAG TCGCAGACTG GATCCCTGGA GCAGCTCCAA
    GTGAGCACCC CATCCAGTGC AACAATGGAT TCAGCCCCTC AGTCTTCTCC AGTAGGTCGC
    TGGCGGTTCT TCATTAACCA GACTATTCGT CACCGTGATT CGCACTCCAA CCAGGGAGCT
    TCGACTCCCC CTCCAGTGGG GGAGAACAGA GTTCCCAAGT CCATAGAAAC CGAGAAAGAT
    CATGAGAGCC CGCAGCGCTC CGAGTCTTTT GCTGGAATAG CATCTTCCCC AAGTTCTTCA
    CTATCTGCAT CCTCTCCTCC AGTCTCTACT GTATCCGCTC CTGCATCCAT GTCAGCCTCA
    GCCATGGCTG CTCAAAAAGA TTTAACACCT GCTGCCTTGC CAGCAGTAGA GAAAGTCTCC
    TCTACTATGC TGACCGCAGG TCCAGTCCCT GTCATGGCCT CCAGCACTTC TGCCACTCCT
    TTTCCTGCAG CCATGGCCCT CACAGGCTCT AATGTGGACC AAGGAGAATC TGCTGTTAAA
    GGTGAGGGTC AACAGCCTTA TGTGACTCCT CCAACGTCAC TGTTGACCCA TGCTGACCCA
    TCTCAGCACT TAATTGTTCA AACCCAGATG GCACCCTCAC AGATGCCAGA TATACCTCAA
    CAAAGCCAGA CACTTCAATC AACATTGATC ACAACTGAAC AAGTTCAGCA ACCGCAGATT
    ACCCCTCAGC CACTTCTGCA GGACGCTCCA CAGCTACCTA AACCAGAATC AAAATCGCTA
    CCACAGGCAG TGCCTAAAGA GATGGTTCCT TTGCATGAAG GTCTTTCAGC TGCCCAAGTT
    CAGCAGGTCC CTCTCCAGCA AGTACCAGTA GAATCGCAAC AGCAACCAAT GCAACAGACC
    ACATTCTTAC AGCAAACATC CAGTGCAGAG CAGATCCAGC AGCACATGGC AGAACAGTTT
    TTATCTCAAG AAACCAAATC AGCTCCAATG ATACTGAACA TGCATCCACA GCATGGCTTC
    CAGACCCAGG ATCCTCAGCA GGTTCAAGCT CCATTGCAGT CTGTGATCCC CCAGCAGGCT
    CCAATTGAGC AAACCCAGAC ATTATATAAA CAGCTCCCAC ATGATATGAT GCCAGTGGCA
    AAAACCGCAG TGCAGAAGCA AGTGTCTTTC CAGCAGTCAG AGTCTGAGCT GTCTACGGGT
    GAGGACACCG GCAGCCACTC TGCACTTCCG CATCCCTCTT TAGACACCTC TCTTCCACCC
    CTCCCTCTCT CTGAAACAGC TCTTCCTGCC CTCTCCCACA CATTCACTCC TTCTCCAGCA
    CAGCCTTCTT CAGTAGCAGA GTCGGACAGT GAAGGCCCTC CCAAAATTGA GTTTGTGGAT
    AACCGAATCA AGACTTTGGA TGAGAAATTG AGGACTCTGC TCTATCAGGA GCACGGCGGC
    AGTGGAGCAG CTGTGGGCAG TGCCTCCACA GGAGGAACTG CTCCAGCCTG CTCCACTTCA
    GGATCTGCCA CAGCCTCTAC CGCAGTTGAT GAGTCTTCAG AAGTCCCCTA CGCATTCTCC
    CTACCTCCTG CCACCTCCTC GGACACCTCC CCTCACTCCA CCTCTTCCAC AACCTCCTCC
    ACCACCCCTC GCTCTTCCTC CACCTCCCCG GATGGAGAGC GAGAGAAAGC AGGCGAGGAG
    ACGACGACTA TCCCACCTGC GGTACAACAA CAGCCTGCCT CCTCTACATC CCCTCCATGC
    TCTCTTCTTT CCCATCCACA GGAGCCTGGA TCCCCAAAAG TTACTATTCA ATCATCAGTA
    AGCGCTCCCC CTTCCCTCTC TGACAGCTGC ACCCCTGGAG ATGTTCTGTT GCCTTCCTCT
    TCTCAGCCGC CCGTCCCACT GTGGCCTGGA CAGCAGCAGC ACAATGAAGG AGGTGGATAT
    TTTGGCCTAA ACCTGACATG TCCTAGTATC AGAAATCCTG TTAGCAAGAA ATCCTGGACT
    CGCAAATTCA AAAGCTGGGC ATGCAAACTG CGCCACTCCG CCAGCTTGTT CAAGAAGCCC
    AGAGTCCAGC AAGATGGCAA CTTTAGCCAG GGGATGAAGG AGGCATCTAC AGATGACCAA
    CCCTGCTCAC GCAGAGGCCG TTTCCAGGTG ATTCCAGTAT CCCAGTCTGC AGAGACCCTT
    CCAACGCAGG AAGCTCCACC AAGCCAAATG ATGAGAGTTG GGCGTTTCTC AGTCACTCAA
    CCTGAACATC GAGAGGAGCA TTTGACCGAC AGCTCCCCTG TGTCACCTGA CCTGGAGCGA
    GAGAGGAGGA AAACGAAAGA CGAGAGGAGA ACGACAGCAC CCAGCCACCC ATCCAAGAGC
    CACGCGCACT CACCCCTGGG CAGCAGTGAT GATGAGAGCG AGGTGGAGGA TGAAGACTTA
    AGGAAAGAAC TGCACAAACT CAGAGAGAAA CACATCAAGG AGGTGGTGTC CCTGCAGGCA
    CAGCAGAACA GAGAACTACA GGATTTGTAC AAGCAGCTCC GCTCTCTCAA AGACCACAGA
    CAGCCAATGT CTATGAGCCT GTCCCGCACC TCTCCTCTGC CCTCCGCACC CCTCGCTATC
    TCCCCGCGCA GACCCCGACC CGCCAAAGCC AAGCTCCGGC CTCGGCCGCA CTCTCACATG
    GATAACAACG GAGTCGCACA CCAAGGGAGT CTTCAGCAGT CTAGCAGTTA TTCCGGTGGG
    GAGCAAAGCC GGCTACATTC ACACTGCAAG CCAGAGAAAT CACAATCCCT GACATCTAAC
    AGAGACCACA GTCCTTCAGC CCAGGGTTCT GGCAACAGAA AGAGCACATT CACAGATGAG
    CTGCATAAAC TTGTCGATGA GTGGTCAAAG GAAACTGTTT GTCCAGTTCA GCCCAAGCCT
    TCGCTGAATC AGATTAAACA GATCCAACAA GTGCAGGAGT TGGGTGGATG GAGCCAGCCT
    GCTGAGACAA CAGCTTCGTC GGGTTGGTTT TCCGGGGCCC CTTTAAGTAC GCCTGTATCA
    GTCAGCATGA CCACCATGAC ATCCACCCCC TATAGCGCCA TTAGTGGACA AACACAGCCG
    CCTCCGACGC AATTCCCTAT CGTTCCTCTA CCCCAGCAGA ACGTGCATCC ACAACCCGGC
    CCGCACCGTC AGCCCCTGCA GTACGGCCCT TCACCCCTGC AGCAGGCCCA GCTCCCGGTG
    CCTGCAGCAG GACAAACACC GCCCCCAACC AATCCAGACG CTGTATTCTG CTCATGCTCT
    TCCTCCTCCT GCTCCTCATC CTCCTGTTCC ACCTCCAGTG CCAAACTTCA CCCCAAACCC
    TCCACGTCCA CCCTTCCTTT AGGACAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295204.2
    CDS1100..6952
    Translation

    Target ORF information:

    RefSeq Version XM_009296929.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296929.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGGAAA GCCTAATTCA CTCTGCCAAC AGGGGATCTT TATTATGCTC TGCTCCTACC
    AATGGAGATG AGGGCCAGTT GGTGTGGCCT AGTGGAAAAC TAGATAAGGC AAAATGTCAG
    AGGAGATCAT CCTGTCAGAA GACTGAAAAA ACTCACTTCC AACTCTGCAT GTTGCAGGTC
    TCATGCACAG GAGACAACAT GGTAGAATGC CAGTTGGAGA CTCACAGCAA CAAGATGGTC
    ACGTTCAAGT TTGACACTGA GGGAGATGCA CCTGAAGACA TTGCAGATTA CATGGTGGAG
    GAGGACTTCG TCCTTGAGCC TGAAAAAGAA AAATTTGTTG AGGAGCTGAG ATCTATAGTG
    AAGAAAGCTC AAGCAATATT AAGTACTCAG TCGCAGACTG GATCCCTGGA GCAGCTCCAA
    GTGAGCACCC CATCCAGTGC AACAATGGAT TCAGCCCCTC AGTCTTCTCC AGTAGGTCGC
    TGGCGGTTCT TCATTAACCA GACTATTCGT CACCGTGATT CGCACTCCAA CCAGGGAGCT
    TCGACTCCCC CTCCAGTGGG GGAGAACAGA GTTCCCAAGT CCATAGAAAC CGAGAAAGAT
    CATGAGAGCC CGCAGCGCTC CGAGTCTTTT GCTGGAATAG CATCTTCCCC AAGTTCTTCA
    CTATCTGCAT CCTCTCCTCC AGTCTCTACT GTATCCGCTC CTGCATCCAT GTCAGCCTCA
    GCCATGGCTG CTCAAAAAGA TTTAACACCT GCTGCCTTGC CAGCAGTAGA GAAAGTCTCC
    TCTACTATGC TGACCGCAGG TCCAGTCCCT GTCATGGCCT CCAGCACTTC TGCCACTCCT
    TTTCCTGCAG CCATGGCCCT CACAGGCTCT AATGTGGACC AAGGAGAATC TGCTGTTAAA
    GGTGAGGGTC AACAGCCTTA TGTGACTCCT CCAACGTCAC TGTTGACCCA TGCTGACCCA
    TCTCAGCACT TAATTGTTCA AACCCAGATG GCACCCTCAC AGATGCCAGA TATACCTCAA
    CAAAGCCAGA CACTTCAATC AACATTGATC ACAACTGAAC AAGTTCAGCA ACCGCAGATT
    ACCCCTCAGC CACTTCTGCA GGACGCTCCA CAGCTACCTA AACCAGAATC AAAATCGCTA
    CCACAGGCAG TGCCTAAAGA GATGGTTCCT TTGCATGAAG GTCTTTCAGC TGCCCAAGTT
    CAGCAGGTCC CTCTCCAGCA AGTACCAGTA GAATCGCAAC AGCAACCAAT GCAACAGACC
    ACATTCTTAC AGCAAACATC CAGTGCAGAG CAGATCCAGC AGCACATGGC AGAACAGTTT
    TTATCTCAAG AAACCAAATC AGCTCCAATG ATACTGAACA TGCATCCACA GCATGGCTTC
    CAGACCCAGG ATCCTCAGCA GGTTCAAGCT CCATTGCAGT CTGTGATCCC CCAGCAGGCT
    CCAATTGAGC AAACCCAGAC ATTATATAAA CAGCTCCCAC ATGATATGAT GCCAGTGGCA
    AAAACCGCAG TGCAGAAGCA AGTGTCTTTC CAGCAGTCAG AGTCTGAGCT GTCTACGGGT
    GAGGACACCG GCAGCCACTC TGCACTTCCG CATCCCTCTT TAGACACCTC TCTTCCACCC
    CTCCCTCTCT CTGAAACAGC TCTTCCTGCC CTCTCCCACA CATTCACTCC TTCTCCAGCA
    CAGCCTTCTT CAGTAGCAGA GTCGGACAGT GAAGGCCCTC CCAAAATTGA GTTTGTGGAT
    AACCGAATCA AGACTTTGGA TGAGAAATTG AGGACTCTGC TCTATCAGGA GCACGGCGGC
    AGTGGAGCAG CTGTGGGCAG TGCCTCCACA GGAGGAACTG CTCCAGCCTG CTCCACTTCA
    GGATCTGCCA CAGCCTCTAC CGCAGTTGAT GAGTCTTCAG AAGTCCCCTA CGCATTCTCC
    CTACCTCCTG CCACCTCCTC GGACACCTCC CCTCACTCCA CCTCTTCCAC AACCTCCTCC
    ACCACCCCTC GCTCTTCCTC CACCTCCCCG GATGGAGAGC GAGAGAAAGC AGGCGAGGAG
    ACGACGACTA TCCCACCTGC GGTACAACAA CAGCCTGCCT CCTCTACATC CCCTCCATGC
    TCTCTTCTTT CCCATCCACA GGAGCCTGGA TCCCCAAAAG TTACTATTCA ATCATCAGTA
    AGCGCTCCCC CTTCCCTCTC TGACAGCTGC ACCCCTGGAG ATGTTCTGTT GCCTTCCTCT
    TCTCAGCCGC CCGTCCCACT GTGGCCTGGA CAGCAGCAGC ACAATGAAGG AGGTGGATAT
    TTTGGCCTAA ACCTGACATG TCCTAGTATC AGAAATCCTG TTAGCAAGAA ATCCTGGACT
    CGCAAATTCA AAAGCTGGGC ATGCAAACTG CGCCACTCCG CCAGCTTGTT CAAGAAGCCC
    AGAGTCCAGC AAGATGGCAA CTTTAGCCAG GGGATGAAGG AGGCATCTAC AGATGACCAA
    CCCTGCTCAC GCAGAGGCCG TTTCCAGGTG ATTCCAGTAT CCCAGTCTGC AGAGACCCTT
    CCAACGCAGG AAGCTCCACC AAGCCAAATG ATGAGAGTTG GGCGTTTCTC AGTCACTCAA
    CCTGAACATC GAGAGGAGCA TTTGACCGAC AGCTCCCCTG TGTCACCTGA CCTGGAGCGA
    GAGAGGAGGA AAACGAAAGA CGAGAGGAGA ACGACAGCAC CCAGCCACCC ATCCAAGAGC
    CACGCGCACT CACCCCTGGG CAGCAGTGAT GATGAGAGCG AGGTGGAGGA TGAAGACTTA
    AGGAAAGAAC TGCACAAACT CAGAGAGAAA CACATCAAGG AGGTGGTGTC CCTGCAGGCA
    CAGCAGAACA GAGAACTACA GGATTTGTAC AAGCAGCTCC GCTCTCTCAA AGACCACAGA
    CAGCCAATGT CTATGAGCCT GTCCCGCACC TCTCCTCTGC CCTCCGCACC CCTCGCTATC
    TCCCCGCGCA GACCCCGACC CGCCAAAGCC AAGCTCCGGC CTCGGCCGCA CTCTCACATG
    GATAACAACG GAGTCGCACA CCAAGGGAGT CTTCAGCAGT CTAGCAGTTA TTCCGGTGGG
    GAGCAAAGCC GGCTACATTC ACACTGCAAG CCAGAGAAAT CACAATCCCT GACATCTAAC
    AGAGACCACA GTCCTTCAGC CCAGGGTTCT GGCAACAGAA AGAGCACATT CACAGATGAG
    CTGCATAAAC TTGTCGATGA GTGGTCAAAG GAAACTGTTT GTCCAGTTCA GCCCAAGCCT
    TCGCTGAATC AGATTAAACA GATCCAACAA GTGCAGGAGT TGGGTGGATG GAGCCAGCCT
    GCTGAGACAA CAGCTTCGTC GGGTTGGTTT TCCGGGGCCC CTTTAAGTAC GCCTGTATCA
    GTCAGCATGA CCACCATGAC ATCCACCCCC TATAGCGCCA TTAGTGGACA AACACAGCCG
    CCTCCGACGC AATTCCCTAT CGTTCCTCTA CCCCAGCAGA ACGTGCATCC ACAACCCGGC
    CCGCACCGTC AGCCCCTGCA GTACGGCCCT TCACCCCTGC AGCAGGCCCA GCTCCCGGTG
    CCTGCAGCAG GACAAACACC GCCCCCAACC AATCCAGACG CTGTATTCTG CTCATGCTCT
    TCCTCCTCCT GCTCCTCATC CTCCTGTTCC ACCTCCAGTG CCAAACTTCA CCCCAAACCC
    TCCACGTCCA CCCTTCCTTT AGGACAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146284
    Clone ID Related Accession (Same CDS sequence) XM_009296926.3
    Accession Version XM_009296926.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395152.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGCCCC TTCCACCTTA CTCAGCAGAT GTTGCATCCT CACAAATGTT ACACTCCCGG
    CCTACTGATG CCTCCACTCC CCACAGTTCC CTAATACAGT CACAGACATA CATGCCCCCA
    GTATCGCCCC AGGCACCCAT GGCTGTCCTT ACGTCCTCTA TTCCGACTGG AGAACCAATA
    GCACCAGCAG GCAACATCAT GCCACCCATA CAGACCCAAA CTAGTATTCC TCCTCTTCAA
    CCCATGGATG TTTTGCCCCT GCAAACTGTT GTTCAAAATC AACAGCAAGT GATGCCTGAA
    TTGCCCAGAA CGCTGCAACC TAACATCCAG CAACCAACAC CACAACAGCA GGCAAATGTA
    ATGCAGCAGC ATCAGACAGT CTTGTTGCCA CAGCAAACTG ACCAGGTTCA GCAAATTAAA
    GTTTTGTTAC AACAGTCACA GCATGCAGAA CAACCCCAGG TGGTTCTGCA GGAGCAGGCC
    CCACATTCCA GTGCATCTCA ACAGCCATAT ATCCCATTAC CACAGCAAGC ACCGCCAACA
    ATGACCAACC CACAGCAAAG TGAGCTGCAA CAGCAGGTGT TTATACAGCA ACCTCAAAGC
    AAACCCCAAC AACAAGTTGG GATACAGCCT CAAGTTCAGC AGCATCAGAC ATCAATACAG
    CAACCCTCAG TTGATCAGTA TCAACACCAG ACTCAACAAC AGCAAGTGTA TGTGCCACAA
    ATAGCAGATC CACAAGACCA AAACGTGATA AAGACTGTGA TACAACAAGT ACCGGTGCTG
    CAAGCAATTC AGCAGCCATT ACAATTGATA GAGCAACAAC AGCAGCAGCT TCTGATTAGG
    CAACAAATTG AGCAACATCA GAAGGCTGCT TTACAAAACC AAATGGAGCA ACATCAACAG
    CAAGTTTATA TTCAGCCACC ACAACAGCAA AAAGTGGAAC AACAACAAAT TCTTATGCAG
    CAACAGCAAA ACTTGGCAGT AAAGCAACAG ATAGAGCAAC AGCAGCATGC ATTAAAGCAA
    CAAAAGCAAA TAGAGCAGCA GCAAAAAGCC ATTTTACAGC AACAGGCAGA ACAGCAAAGA
    CAGCAGCAAC AGACTTTGTT TCAGCAACAG CAGCTGGAGC AGCAACAAGT ATTACTGCAG
    CAGCAACAGC GTGAACAACA ACAGGCTCTT CTCCAGCAGC AACAGTTGGA GCAGCAAGCT
    TTACTGCAAC AACAGCAGCA ACTTCAGTTA AAGCAGCAAC AAGCTCTTCT ACATCAACAA
    CAGCTGGAGC AGCAGCAGCA GGCTTTGTTT AAACAGCAGC AGCTGGAGCA ACAGCAAGCT
    CTTTTGCAGC AGCAGCAGCA GCAAGCTCTC GTTCAGCAGC AGCAACAGTT AGAGCAAAAA
    GTACTGCTAC AGCTGCAACA GCAACAGCAG CAACACATCG ATCAGCAGCA AAATTTAATA
    CGCCAACAGC AGCCTCAGCA GATCGAGGCT GTTTTACAGC AACAACCACA ACTTCAGAAC
    TCAACTGATC AACAATTGCA GCAGCAAATA TTTCAACAAC AGACCGAGCA GCAACAGATG
    CAGCAGCATC TACTGATGAA ACAACAGCAG ATGGAATTAC AGCGACAACA AGCTCTACAA
    GCTGAGAAAC AACGACAGCA AGTTCTATTG CAACAACAAC ATTTGGAGCA ACATGCAGCT
    CTGGTGGATC ATAGTGGGCA GCAACAAGCA ACCCTGGTGA AAGCACAGTC TCCAGAAATG
    GTCCAGCAGA TTAGTCAAGT GCAGCAATCT CAACAAATGA TGTCTCTACA ACCGCAGCCT
    CATCTTGCTC AACAACAAAC TGACCAGACA CAGGTCTTGA CTCAGCAACA AGCCATTCTT
    CCCCAAGCAA CACACAGACC TTCTCTGGTA GACTTGCAAA TCCCAACCCT GCTTCAAGCA
    GCACCTAAAA TAGTCAGCAC TCAAATACCT ACCCAAGTTC AAGCCCCAGT ACTTTTGCAG
    CAACAGGTGC CACCTCAGAT GTCAGCTCAA CCTACAGCAC AAGTACAACT CCAGAGTCAA
    GGTGAACCAA CAATTTATAA CCAGATTGCA ATGCAGAGTC AAAGTCATCC CGCACAGCAG
    TCAATGGGAG GTCAAATTCA GACAGGCCAA TTGCAAATTC CACAATCAAC CCAACAAATC
    ACATCCCCAA CACAAGTTCC TACACCACAA CCCATTCATT CTTTTGTTCC AACTTCAACA
    CAGATGGTCT CACAAGGGCA AACCCTTCCA CCTCAACCTC AACTGGTACC CCCTCAAGCT
    GCAGCTTCTC TCCAGTGTCA AGTTGCAGTA GGGTCATCTG CACCTGCATC TTTTGTACAA
    GCTACAGTCC CAACTGCTTC TACACTTGTG CCCACACAGT ATGCTCAAGC ACCTGTTGCT
    TCTCAAACGA TCCAGTCTGT TCAGTTAGAC CTCACTCAAC CTGTGAAGCA GCAACAACAA
    CAACAGCCGC CTCTGCAGCA ATATCAGCAA GCGGTGCTAT CTCCGGTGTC ACTTGCACCT
    ACGATCAATC CAGTGGCTTC TTCCTCAACA CAGTGCTATT TACAGCAGAC AAACCAATCA
    CAAATGCAGG TCCAAACCCA ACAAGTAATT CAAGCTCAGC AACAACCAGT CAGTTTGGCC
    TCGATGCAGG CAGTCTTGCA GCCCCTGCCT TTAGTGCAGC CTCAGGCTAC CCAACAGTAT
    GAGCAGCATT TGCCTCAAGC CCACCATGTG CAACAACAAC CCCTCCACCA TCAGCAGCAG
    CTTCTGCCCC CAAATGCTGT TATACCACAA GATATCCACC AACAAACTTG TCAGCCTCAA
    CCAGTGCTCC AGCATGTGCT CCCATTACAC ACTGCACCCA GTCAGGTACC TCAGTCCCAA
    GCTAGTACAG TACCTCTCTA CAGTCAGGTG GTGTCAGCTT CCCCCCAGCA GAAATTAACT
    CTTCACACTC AAACACAGTC TAGCATGCAG TCACAAGTGC TCTCAAACTC ACAGGCACAG
    ACTCACATAG AGCCCACTGG AGCATTTGAG CATCCTCATT ACACCCAGGC TGCCTTTATG
    CCATCACAGA TTCCCACAAG TCCATCTCAT ACTTCCATAC AGCCCTCTTC CACTCTCCCA
    GCCCAACAAC CAGTTGTCCC AGTTTCAGAA ATTCCTGTCC CAGCAGACGC ACAGCTAACA
    CAGGCAAGGC CTGCTGATTT GATCCCTGCT TCCACTCCTA CTGTCAATAC TACCCAGTCC
    CATGACTCAA ACGGCTCTGT GCTGACAACC GCCATGCTGG CAGAAAGTGA CGCTGCATTG
    CTGGGCATTG CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGCC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACACTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TCTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC GCACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTAACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCAGATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GACGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATCGCAACA GCAACCAATG
    CAACAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATCCAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGAACAT GCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TGTGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGCTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGG CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACC GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCAAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGATGGCAAC TTTAGCCAGG GGATGAAGGA GGCATCTACA
    GATGACCAAC CCTGCTCACG CAGAGGCCGT TTCCAGGTGA TTCCAGTATC CCAGTCTGCA
    GAGACCCTTC CAACGCAGGA AGCTCCACCA AGCCAAATGA TGAGAGTTGG GCGTTTCTCA
    GTCACTCAAC CTGAACATCG AGAGGAGCAT TTGACCGACA GCTCCCCTGT GTCACCTGAC
    CTGGAGCGAG AGAGGAGGAA AACGAAAGAC GAGAGGAGAA CGACAGCACC CAGCCACCCA
    TCCAAGAGCC ACGCGCACTC ACCCCTGGGC AGCAGTGATG ATGAGAGCGA GGTGGAGGAT
    GAAGACTTAA GGAAAGAACT GCACAAACTC AGAGAGAAAC ACATCAAGGA GGTGGTGTCC
    CTGCAGGCAC AGCAGAACAG AGAACTACAG GATTTGTACA AGCAGCTCCG CTCTCTCAAA
    GACCACAGAC AGCCAATGTC TATGAGCCTG TCCCGCACCT CTCCTCTGCC CTCCGCACCC
    CTCGCTATCT CCCCGCGCAG ACCCCGACCC GCCAAAGCCA AGCTCCGGCC TCGGCCGCAC
    TCTCACATGG ATAACAACGG AGTCGCACAC CAAGGGAGTC TTCAGCAGTC TAGCAGTTAT
    TCCGGTGGGG AGCAAAGCCG GCTACATTCA CACTGCAAGC CAGAGAAATC ACAATCCCTG
    ACATCTAACA GAGACCACAG TCCTTCAGCC CAGGGTTCTG GCAACAGAAA GAGCACATTC
    ACAGATGAGC TGCATAAACT TGTCGATGAG TGGTCAAAGG AAACTGTTTG TCCAGTTCAG
    CCCAAGCCTT CGCTGAATCA GATTAAACAG ATCCAACAAG TGCAGGAGTT GGGTGGATGG
    AGCCAGCCTG CTGAGACAAC AGCTTCGTCG GGTTGGTTTT CCGGGGCCCC TTTAAGTACG
    CCTGTATCAG TCAGCATGAC CACCATGACA TCCACCCCCT ATAGCGCCAT TAGTGGACAA
    ACACAGCCGC CTCCGACGCA ATTCCCTATC GTTCCTCTAC CCCAGCAGAA CGTGCATCCA
    CAACCCGGCC CGCACCGTCA GCCCCTGCAG TACGGCCCTT CACCCCTGCA GCAGGCCCAG
    CTCCCGGTGC CTGCAGCAGG ACAAACACCG CCCCCAACCA ATCCAGACGC TGTATTCTGC
    TCATGCTCTT CCTCCTCCTG CTCCTCATCC TCCTGTTCCA CCTCCAGTGC CAAACTTCAC
    CCCAAACCCT CCACGTCCAC CCTTCCTTTA GGACAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295201.2
    CDS1064..10225
    Translation

    Target ORF information:

    RefSeq Version XM_009296926.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296926.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGCCCC TTCCACCTTA CTCAGCAGAT GTTGCATCCT CACAAATGTT ACACTCCCGG
    CCTACTGATG CCTCCACTCC CCACAGTTCC CTAATACAGT CACAGACATA CATGCCCCCA
    GTATCGCCCC AGGCACCCAT GGCTGTCCTT ACGTCCTCTA TTCCGACTGG AGAACCAATA
    GCACCAGCAG GCAACATCAT GCCACCCATA CAGACCCAAA CTAGTATTCC TCCTCTTCAA
    CCCATGGATG TTTTGCCCCT GCAAACTGTT GTTCAAAATC AACAGCAAGT GATGCCTGAA
    TTGCCCAGAA CGCTGCAACC TAACATCCAG CAACCAACAC CACAACAGCA GGCAAATGTA
    ATGCAGCAGC ATCAGACAGT CTTGTTGCCA CAGCAAACTG ACCAGGTTCA GCAAATTAAA
    GTTTTGTTAC AACAGTCACA GCATGCAGAA CAACCCCAGG TGGTTCTGCA GGAGCAGGCC
    CCACATTCCA GTGCATCTCA ACAGCCATAT ATCCCATTAC CACAGCAAGC ACCGCCAACA
    ATGACCAACC CACAGCAAAG TGAGCTGCAA CAGCAGGTGT TTATACAGCA ACCTCAAAGC
    AAACCCCAAC AACAAGTTGG GATACAGCCT CAAGTTCAGC AGCATCAGAC ATCAATACAG
    CAACCCTCAG TTGATCAGTA TCAACACCAG ACTCAACAAC AGCAAGTGTA TGTGCCACAA
    ATAGCAGATC CACAAGACCA AAACGTGATA AAGACTGTGA TACAACAAGT ACCGGTGCTG
    CAAGCAATTC AGCAGCCATT ACAATTGATA GAGCAACAAC AGCAGCAGCT TCTGATTAGG
    CAACAAATTG AGCAACATCA GAAGGCTGCT TTACAAAACC AAATGGAGCA ACATCAACAG
    CAAGTTTATA TTCAGCCACC ACAACAGCAA AAAGTGGAAC AACAACAAAT TCTTATGCAG
    CAACAGCAAA ACTTGGCAGT AAAGCAACAG ATAGAGCAAC AGCAGCATGC ATTAAAGCAA
    CAAAAGCAAA TAGAGCAGCA GCAAAAAGCC ATTTTACAGC AACAGGCAGA ACAGCAAAGA
    CAGCAGCAAC AGACTTTGTT TCAGCAACAG CAGCTGGAGC AGCAACAAGT ATTACTGCAG
    CAGCAACAGC GTGAACAACA ACAGGCTCTT CTCCAGCAGC AACAGTTGGA GCAGCAAGCT
    TTACTGCAAC AACAGCAGCA ACTTCAGTTA AAGCAGCAAC AAGCTCTTCT ACATCAACAA
    CAGCTGGAGC AGCAGCAGCA GGCTTTGTTT AAACAGCAGC AGCTGGAGCA ACAGCAAGCT
    CTTTTGCAGC AGCAGCAGCA GCAAGCTCTC GTTCAGCAGC AGCAACAGTT AGAGCAAAAA
    GTACTGCTAC AGCTGCAACA GCAACAGCAG CAACACATCG ATCAGCAGCA AAATTTAATA
    CGCCAACAGC AGCCTCAGCA GATCGAGGCT GTTTTACAGC AACAACCACA ACTTCAGAAC
    TCAACTGATC AACAATTGCA GCAGCAAATA TTTCAACAAC AGACCGAGCA GCAACAGATG
    CAGCAGCATC TACTGATGAA ACAACAGCAG ATGGAATTAC AGCGACAACA AGCTCTACAA
    GCTGAGAAAC AACGACAGCA AGTTCTATTG CAACAACAAC ATTTGGAGCA ACATGCAGCT
    CTGGTGGATC ATAGTGGGCA GCAACAAGCA ACCCTGGTGA AAGCACAGTC TCCAGAAATG
    GTCCAGCAGA TTAGTCAAGT GCAGCAATCT CAACAAATGA TGTCTCTACA ACCGCAGCCT
    CATCTTGCTC AACAACAAAC TGACCAGACA CAGGTCTTGA CTCAGCAACA AGCCATTCTT
    CCCCAAGCAA CACACAGACC TTCTCTGGTA GACTTGCAAA TCCCAACCCT GCTTCAAGCA
    GCACCTAAAA TAGTCAGCAC TCAAATACCT ACCCAAGTTC AAGCCCCAGT ACTTTTGCAG
    CAACAGGTGC CACCTCAGAT GTCAGCTCAA CCTACAGCAC AAGTACAACT CCAGAGTCAA
    GGTGAACCAA CAATTTATAA CCAGATTGCA ATGCAGAGTC AAAGTCATCC CGCACAGCAG
    TCAATGGGAG GTCAAATTCA GACAGGCCAA TTGCAAATTC CACAATCAAC CCAACAAATC
    ACATCCCCAA CACAAGTTCC TACACCACAA CCCATTCATT CTTTTGTTCC AACTTCAACA
    CAGATGGTCT CACAAGGGCA AACCCTTCCA CCTCAACCTC AACTGGTACC CCCTCAAGCT
    GCAGCTTCTC TCCAGTGTCA AGTTGCAGTA GGGTCATCTG CACCTGCATC TTTTGTACAA
    GCTACAGTCC CAACTGCTTC TACACTTGTG CCCACACAGT ATGCTCAAGC ACCTGTTGCT
    TCTCAAACGA TCCAGTCTGT TCAGTTAGAC CTCACTCAAC CTGTGAAGCA GCAACAACAA
    CAACAGCCGC CTCTGCAGCA ATATCAGCAA GCGGTGCTAT CTCCGGTGTC ACTTGCACCT
    ACGATCAATC CAGTGGCTTC TTCCTCAACA CAGTGCTATT TACAGCAGAC AAACCAATCA
    CAAATGCAGG TCCAAACCCA ACAAGTAATT CAAGCTCAGC AACAACCAGT CAGTTTGGCC
    TCGATGCAGG CAGTCTTGCA GCCCCTGCCT TTAGTGCAGC CTCAGGCTAC CCAACAGTAT
    GAGCAGCATT TGCCTCAAGC CCACCATGTG CAACAACAAC CCCTCCACCA TCAGCAGCAG
    CTTCTGCCCC CAAATGCTGT TATACCACAA GATATCCACC AACAAACTTG TCAGCCTCAA
    CCAGTGCTCC AGCATGTGCT CCCATTACAC ACTGCACCCA GTCAGGTACC TCAGTCCCAA
    GCTAGTACAG TACCTCTCTA CAGTCAGGTG GTGTCAGCTT CCCCCCAGCA GAAATTAACT
    CTTCACACTC AAACACAGTC TAGCATGCAG TCACAAGTGC TCTCAAACTC ACAGGCACAG
    ACTCACATAG AGCCCACTGG AGCATTTGAG CATCCTCATT ACACCCAGGC TGCCTTTATG
    CCATCACAGA TTCCCACAAG TCCATCTCAT ACTTCCATAC AGCCCTCTTC CACTCTCCCA
    GCCCAACAAC CAGTTGTCCC AGTTTCAGAA ATTCCTGTCC CAGCAGACGC ACAGCTAACA
    CAGGCAAGGC CTGCTGATTT GATCCCTGCT TCCACTCCTA CTGTCAATAC TACCCAGTCC
    CATGACTCAA ACGGCTCTGT GCTGACAACC GCCATGCTGG CAGAAAGTGA CGCTGCATTG
    CTGGGCATTG CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGCC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACACTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TCTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC GCACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTAACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCAGATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GACGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATCGCAACA GCAACCAATG
    CAACAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATCCAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGAACAT GCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TGTGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGCTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGG CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACC GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCAAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGATGGCAAC TTTAGCCAGG GGATGAAGGA GGCATCTACA
    GATGACCAAC CCTGCTCACG CAGAGGCCGT TTCCAGGTGA TTCCAGTATC CCAGTCTGCA
    GAGACCCTTC CAACGCAGGA AGCTCCACCA AGCCAAATGA TGAGAGTTGG GCGTTTCTCA
    GTCACTCAAC CTGAACATCG AGAGGAGCAT TTGACCGACA GCTCCCCTGT GTCACCTGAC
    CTGGAGCGAG AGAGGAGGAA AACGAAAGAC GAGAGGAGAA CGACAGCACC CAGCCACCCA
    TCCAAGAGCC ACGCGCACTC ACCCCTGGGC AGCAGTGATG ATGAGAGCGA GGTGGAGGAT
    GAAGACTTAA GGAAAGAACT GCACAAACTC AGAGAGAAAC ACATCAAGGA GGTGGTGTCC
    CTGCAGGCAC AGCAGAACAG AGAACTACAG GATTTGTACA AGCAGCTCCG CTCTCTCAAA
    GACCACAGAC AGCCAATGTC TATGAGCCTG TCCCGCACCT CTCCTCTGCC CTCCGCACCC
    CTCGCTATCT CCCCGCGCAG ACCCCGACCC GCCAAAGCCA AGCTCCGGCC TCGGCCGCAC
    TCTCACATGG ATAACAACGG AGTCGCACAC CAAGGGAGTC TTCAGCAGTC TAGCAGTTAT
    TCCGGTGGGG AGCAAAGCCG GCTACATTCA CACTGCAAGC CAGAGAAATC ACAATCCCTG
    ACATCTAACA GAGACCACAG TCCTTCAGCC CAGGGTTCTG GCAACAGAAA GAGCACATTC
    ACAGATGAGC TGCATAAACT TGTCGATGAG TGGTCAAAGG AAACTGTTTG TCCAGTTCAG
    CCCAAGCCTT CGCTGAATCA GATTAAACAG ATCCAACAAG TGCAGGAGTT GGGTGGATGG
    AGCCAGCCTG CTGAGACAAC AGCTTCGTCG GGTTGGTTTT CCGGGGCCCC TTTAAGTACG
    CCTGTATCAG TCAGCATGAC CACCATGACA TCCACCCCCT ATAGCGCCAT TAGTGGACAA
    ACACAGCCGC CTCCGACGCA ATTCCCTATC GTTCCTCTAC CCCAGCAGAA CGTGCATCCA
    CAACCCGGCC CGCACCGTCA GCCCCTGCAG TACGGCCCTT CACCCCTGCA GCAGGCCCAG
    CTCCCGGTGC CTGCAGCAGG ACAAACACCG CCCCCAACCA ATCCAGACGC TGTATTCTGC
    TCATGCTCTT CCTCCTCCTG CTCCTCATCC TCCTGTTCCA CCTCCAGTGC CAAACTTCAC
    CCCAAACCCT CCACGTCCAC CCTTCCTTTA GGACAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146285
    Clone ID Related Accession (Same CDS sequence) XM_690703.9
    Accession Version XM_690703.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395152.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_690703.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    301
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    481
    541
    601
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    721
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
    2221
    2281
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    2581
    2641
    2701
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    3001
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    3121
    3181
    3241
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    4141
    4201
    4261
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    4441
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    4621
    4681
    4741
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    4921
    4981
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    5161
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    5281
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    5641
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    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
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    6601
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    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGCCCC TTCCACCTTA CTCAGCAGAT GTTGCATCCT CACAAATGTT ACACTCCCGG
    CCTACTGATG CCTCCACTCC CCACAGTTCC CTAATACAGT CACAGACATA CATGCCCCCA
    GTATCGCCCC AGGCACCCAT GGCTGTCCTT ACGTCCTCTA TTCCGACTGG AGAACCAATA
    GCACCAGCAG GCAACATCAT GCCACCCATA CAGACCCAAA CTAGTATTCC TCCTCTTCAA
    CCCATGGATG TTTTGCCCCT GCAAACTGTT GTTCAAAATC AACAGCAAGT GATGCCTGAA
    TTGCCCAGAA CGCTGCAACC TAACATCCAG CAACCAACAC CACAACAGCA GGCAAATGTA
    ATGCAGCAGC ATCAGACAGT CTTGTTGCCA CAGCAAACTG ACCAGGTTCA GCAAATTAAA
    GTTTTGTTAC AACAGTCACA GCATGCAGAA CAACCCCAGG TGGTTCTGCA GGAGCAGGCC
    CCACATTCCA GTGCATCTCA ACAGCCATAT ATCCCATTAC CACAGCAAGC ACCGCCAACA
    ATGACCAACC CACAGCAAAG TGAGCTGCAA CAGCAGGTGT TTATACAGCA ACCTCAAAGC
    AAACCCCAAC AACAAGTTGG GATACAGCCT CAAGTTCAGC AGCATCAGAC ATCAATACAG
    CAACCCTCAG TTGATCAGTA TCAACACCAG ACTCAACAAC AGCAAGTGTA TGTGCCACAA
    ATAGCAGATC CACAAGACCA AAACGTGATA AAGACTGTGA TACAACAAGT ACCGGTGCTG
    CAAGCAATTC AGCAGCCATT ACAATTGATA GAGCAACAAC AGCAGCAGCT TCTGATTAGG
    CAACAAATTG AGCAACATCA GAAGGCTGCT TTACAAAACC AAATGGAGCA ACATCAACAG
    CAAGTTTATA TTCAGCCACC ACAACAGCAA AAAGTGGAAC AACAACAAAT TCTTATGCAG
    CAACAGCAAA ACTTGGCAGT AAAGCAACAG ATAGAGCAAC AGCAGCATGC ATTAAAGCAA
    CAAAAGCAAA TAGAGCAGCA GCAAAAAGCC ATTTTACAGC AACAGGCAGA ACAGCAAAGA
    CAGCAGCAAC AGACTTTGTT TCAGCAACAG CAGCTGGAGC AGCAACAAGT ATTACTGCAG
    CAGCAACAGC GTGAACAACA ACAGGCTCTT CTCCAGCAGC AACAGTTGGA GCAGCAAGCT
    TTACTGCAAC AACAGCAGCA ACTTCAGTTA AAGCAGCAAC AAGCTCTTCT ACATCAACAA
    CAGCTGGAGC AGCAGCAGCA GGCTTTGTTT AAACAGCAGC AGCTGGAGCA ACAGCAAGCT
    CTTTTGCAGC AGCAGCAGCA GCAAGCTCTC GTTCAGCAGC AGCAACAGTT AGAGCAAAAA
    GTACTGCTAC AGCTGCAACA GCAACAGCAG CAACACATCG ATCAGCAGCA AAATTTAATA
    CGCCAACAGC AGCCTCAGCA GATCGAGGCT GTTTTACAGC AACAACCACA ACTTCAGAAC
    TCAACTGATC AACAATTGCA GCAGCAAATA TTTCAACAAC AGACCGAGCA GCAACAGATG
    CAGCAGCATC TACTGATGAA ACAACAGCAG ATGGAATTAC AGCGACAACA AGCTCTACAA
    GCTGAGAAAC AACGACAGCA AGTTCTATTG CAACAACAAC ATTTGGAGCA ACATGCAGCT
    CTGGTGGATC ATAGTGGGCA GCAACAAGCA ACCCTGGTGA AAGCACAGTC TCCAGAAATG
    GTCCAGCAGA TTAGTCAAGT GCAGCAATCT CAACAAATGA TGTCTCTACA ACCGCAGCCT
    CATCTTGCTC AACAACAAAC TGACCAGACA CAGGTCTTGA CTCAGCAACA AGCCATTCTT
    CCCCAAGCAA CACACAGACC TTCTCTGGTA GACTTGCAAA TCCCAACCCT GCTTCAAGCA
    GCACCTAAAA TAGTCAGCAC TCAAATACCT ACCCAAGTTC AAGCCCCAGT ACTTTTGCAG
    CAACAGGTGC CACCTCAGAT GTCAGCTCAA CCTACAGCAC AAGTACAACT CCAGAGTCAA
    GGTGAACCAA CAATTTATAA CCAGATTGCA ATGCAGAGTC AAAGTCATCC CGCACAGCAG
    TCAATGGGAG GTCAAATTCA GACAGGCCAA TTGCAAATTC CACAATCAAC CCAACAAATC
    ACATCCCCAA CACAAGTTCC TACACCACAA CCCATTCATT CTTTTGTTCC AACTTCAACA
    CAGATGGTCT CACAAGGGCA AACCCTTCCA CCTCAACCTC AACTGGTACC CCCTCAAGCT
    GCAGCTTCTC TCCAGTGTCA AGTTGCAGTA GGGTCATCTG CACCTGCATC TTTTGTACAA
    GCTACAGTCC CAACTGCTTC TACACTTGTG CCCACACAGT ATGCTCAAGC ACCTGTTGCT
    TCTCAAACGA TCCAGTCTGT TCAGTTAGAC CTCACTCAAC CTGTGAAGCA GCAACAACAA
    CAACAGCCGC CTCTGCAGCA ATATCAGCAA GCGGTGCTAT CTCCGGTGTC ACTTGCACCT
    ACGATCAATC CAGTGGCTTC TTCCTCAACA CAGTGCTATT TACAGCAGAC AAACCAATCA
    CAAATGCAGG TCCAAACCCA ACAAGTAATT CAAGCTCAGC AACAACCAGT CAGTTTGGCC
    TCGATGCAGG CAGTCTTGCA GCCCCTGCCT TTAGTGCAGC CTCAGGCTAC CCAACAGTAT
    GAGCAGCATT TGCCTCAAGC CCACCATGTG CAACAACAAC CCCTCCACCA TCAGCAGCAG
    CTTCTGCCCC CAAATGCTGT TATACCACAA GATATCCACC AACAAACTTG TCAGCCTCAA
    CCAGTGCTCC AGCATGTGCT CCCATTACAC ACTGCACCCA GTCAGGTACC TCAGTCCCAA
    GCTAGTACAG TACCTCTCTA CAGTCAGGTG GTGTCAGCTT CCCCCCAGCA GAAATTAACT
    CTTCACACTC AAACACAGTC TAGCATGCAG TCACAAGTGC TCTCAAACTC ACAGGCACAG
    ACTCACATAG AGCCCACTGG AGCATTTGAG CATCCTCATT ACACCCAGGC TGCCTTTATG
    CCATCACAGA TTCCCACAAG TCCATCTCAT ACTTCCATAC AGCCCTCTTC CACTCTCCCA
    GCCCAACAAC CAGTTGTCCC AGTTTCAGAA ATTCCTGTCC CAGCAGACGC ACAGCTAACA
    CAGGCAAGGC CTGCTGATTT GATCCCTGCT TCCACTCCTA CTGTCAATAC TACCCAGTCC
    CATGACTCAA ACGGCTCTGT GCTGACAACC GCCATGCTGG CAGAAAGTGA CGCTGCATTG
    CTGGGCATTG CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGCC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACACTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TCTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC GCACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTAACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCAGATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GACGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATCGCAACA GCAACCAATG
    CAACAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATCCAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGAACAT GCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TGTGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGCTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGG CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACC GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCAAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GATGGCAACT TTAGCCAGGG GATGAAGGAG GCATCTACAG ATGACCAACC CTGCTCACGC
    AGAGGCCGTT TCCAGGTGAT TCCAGTATCC CAGTCTGCAG AGACCCTTCC AACGCAGGAA
    GCTCCACCAA GCCAAATGAT GAGAGTTGGG CGTTTCTCAG TCACTCAACC TGAACATCGA
    GAGGAGCATT TGACCGACAG CTCCCCTGTG TCACCTGACC TGGAGCGAGA GAGGAGGAAA
    ACGAAAGACG AGAGGAGAAC GACAGCACCC AGCCACCCAT CCAAGAGCCA CGCGCACTCA
    CCCCTGGGCA GCAGTGATGA TGAGAGCGAG GTGGAGGATG AAGACTTAAG GAAAGAACTG
    CACAAACTCA GAGAGAAACA CATCAAGGAG GTGGTGTCCC TGCAGGCACA GCAGAACAGA
    GAACTACAGG ATTTGTACAA GCAGCTCCGC TCTCTCAAAG ACCACAGACA GCCAATGTCT
    ATGAGCCTGT CCCGCACCTC TCCTCTGCCC TCCGCACCCC TCGCTATCTC CCCGCGCAGA
    CCCCGACCCG CCAAAGCCAA GCTCCGGCCT CGGCCGCACT CTCACATGGA TAACAACGGA
    GTCGCACACC AAGGGAGTCT TCAGCAGTCT AGCAGTTATT CCGGTGGGGA GCAAAGCCGG
    CTACATTCAC ACTGCAAGCC AGAGAAATCA CAATCCCTGA CATCTAACAG AGACCACAGT
    CCTTCAGCCC AGGGTTCTGG CAACAGAAAG AGCACATTCA CAGATGAGCT GCATAAACTT
    GTCGATGAGT GGTCAAAGGA AACTGTTTGT CCAGTTCAGC CCAAGCCTTC GCTGAATCAG
    ATTAAACAGA TCCAACAAGT GCAGGAGTTG GGTGGATGGA GCCAGCCTGC TGAGACAACA
    GCTTCGTCGG GTTGGTTTTC CGGGGCCCCT TTAAGTACGC CTGTATCAGT CAGCATGACC
    ACCATGACAT CCACCCCCTA TAGCGCCATT AGTGGACAAA CACAGCCGCC TCCGACGCAA
    TTCCCTATCG TTCCTCTACC CCAGCAGAAC GTGCATCCAC AACCCGGCCC GCACCGTCAG
    CCCCTGCAGT ACGGCCCTTC ACCCCTGCAG CAGGCCCAGC TCCCGGTGCC TGCAGCAGGA
    CAAACACCGC CCCCAACCAA TCCAGACGCT GTATTCTGCT CATGCTCTTC CTCCTCCTGC
    TCCTCATCCT CCTGTTCCAC CTCCAGTGCC AAACTTCACC CCAAACCCTC CACGTCCACC
    CTTCCTTTAG GACAGCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_695795.7
    CDS1064..10084
    Translation

    Target ORF information:

    RefSeq Version XM_690703.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_690703.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGCCCC TTCCACCTTA CTCAGCAGAT GTTGCATCCT CACAAATGTT ACACTCCCGG
    CCTACTGATG CCTCCACTCC CCACAGTTCC CTAATACAGT CACAGACATA CATGCCCCCA
    GTATCGCCCC AGGCACCCAT GGCTGTCCTT ACGTCCTCTA TTCCGACTGG AGAACCAATA
    GCACCAGCAG GCAACATCAT GCCACCCATA CAGACCCAAA CTAGTATTCC TCCTCTTCAA
    CCCATGGATG TTTTGCCCCT GCAAACTGTT GTTCAAAATC AACAGCAAGT GATGCCTGAA
    TTGCCCAGAA CGCTGCAACC TAACATCCAG CAACCAACAC CACAACAGCA GGCAAATGTA
    ATGCAGCAGC ATCAGACAGT CTTGTTGCCA CAGCAAACTG ACCAGGTTCA GCAAATTAAA
    GTTTTGTTAC AACAGTCACA GCATGCAGAA CAACCCCAGG TGGTTCTGCA GGAGCAGGCC
    CCACATTCCA GTGCATCTCA ACAGCCATAT ATCCCATTAC CACAGCAAGC ACCGCCAACA
    ATGACCAACC CACAGCAAAG TGAGCTGCAA CAGCAGGTGT TTATACAGCA ACCTCAAAGC
    AAACCCCAAC AACAAGTTGG GATACAGCCT CAAGTTCAGC AGCATCAGAC ATCAATACAG
    CAACCCTCAG TTGATCAGTA TCAACACCAG ACTCAACAAC AGCAAGTGTA TGTGCCACAA
    ATAGCAGATC CACAAGACCA AAACGTGATA AAGACTGTGA TACAACAAGT ACCGGTGCTG
    CAAGCAATTC AGCAGCCATT ACAATTGATA GAGCAACAAC AGCAGCAGCT TCTGATTAGG
    CAACAAATTG AGCAACATCA GAAGGCTGCT TTACAAAACC AAATGGAGCA ACATCAACAG
    CAAGTTTATA TTCAGCCACC ACAACAGCAA AAAGTGGAAC AACAACAAAT TCTTATGCAG
    CAACAGCAAA ACTTGGCAGT AAAGCAACAG ATAGAGCAAC AGCAGCATGC ATTAAAGCAA
    CAAAAGCAAA TAGAGCAGCA GCAAAAAGCC ATTTTACAGC AACAGGCAGA ACAGCAAAGA
    CAGCAGCAAC AGACTTTGTT TCAGCAACAG CAGCTGGAGC AGCAACAAGT ATTACTGCAG
    CAGCAACAGC GTGAACAACA ACAGGCTCTT CTCCAGCAGC AACAGTTGGA GCAGCAAGCT
    TTACTGCAAC AACAGCAGCA ACTTCAGTTA AAGCAGCAAC AAGCTCTTCT ACATCAACAA
    CAGCTGGAGC AGCAGCAGCA GGCTTTGTTT AAACAGCAGC AGCTGGAGCA ACAGCAAGCT
    CTTTTGCAGC AGCAGCAGCA GCAAGCTCTC GTTCAGCAGC AGCAACAGTT AGAGCAAAAA
    GTACTGCTAC AGCTGCAACA GCAACAGCAG CAACACATCG ATCAGCAGCA AAATTTAATA
    CGCCAACAGC AGCCTCAGCA GATCGAGGCT GTTTTACAGC AACAACCACA ACTTCAGAAC
    TCAACTGATC AACAATTGCA GCAGCAAATA TTTCAACAAC AGACCGAGCA GCAACAGATG
    CAGCAGCATC TACTGATGAA ACAACAGCAG ATGGAATTAC AGCGACAACA AGCTCTACAA
    GCTGAGAAAC AACGACAGCA AGTTCTATTG CAACAACAAC ATTTGGAGCA ACATGCAGCT
    CTGGTGGATC ATAGTGGGCA GCAACAAGCA ACCCTGGTGA AAGCACAGTC TCCAGAAATG
    GTCCAGCAGA TTAGTCAAGT GCAGCAATCT CAACAAATGA TGTCTCTACA ACCGCAGCCT
    CATCTTGCTC AACAACAAAC TGACCAGACA CAGGTCTTGA CTCAGCAACA AGCCATTCTT
    CCCCAAGCAA CACACAGACC TTCTCTGGTA GACTTGCAAA TCCCAACCCT GCTTCAAGCA
    GCACCTAAAA TAGTCAGCAC TCAAATACCT ACCCAAGTTC AAGCCCCAGT ACTTTTGCAG
    CAACAGGTGC CACCTCAGAT GTCAGCTCAA CCTACAGCAC AAGTACAACT CCAGAGTCAA
    GGTGAACCAA CAATTTATAA CCAGATTGCA ATGCAGAGTC AAAGTCATCC CGCACAGCAG
    TCAATGGGAG GTCAAATTCA GACAGGCCAA TTGCAAATTC CACAATCAAC CCAACAAATC
    ACATCCCCAA CACAAGTTCC TACACCACAA CCCATTCATT CTTTTGTTCC AACTTCAACA
    CAGATGGTCT CACAAGGGCA AACCCTTCCA CCTCAACCTC AACTGGTACC CCCTCAAGCT
    GCAGCTTCTC TCCAGTGTCA AGTTGCAGTA GGGTCATCTG CACCTGCATC TTTTGTACAA
    GCTACAGTCC CAACTGCTTC TACACTTGTG CCCACACAGT ATGCTCAAGC ACCTGTTGCT
    TCTCAAACGA TCCAGTCTGT TCAGTTAGAC CTCACTCAAC CTGTGAAGCA GCAACAACAA
    CAACAGCCGC CTCTGCAGCA ATATCAGCAA GCGGTGCTAT CTCCGGTGTC ACTTGCACCT
    ACGATCAATC CAGTGGCTTC TTCCTCAACA CAGTGCTATT TACAGCAGAC AAACCAATCA
    CAAATGCAGG TCCAAACCCA ACAAGTAATT CAAGCTCAGC AACAACCAGT CAGTTTGGCC
    TCGATGCAGG CAGTCTTGCA GCCCCTGCCT TTAGTGCAGC CTCAGGCTAC CCAACAGTAT
    GAGCAGCATT TGCCTCAAGC CCACCATGTG CAACAACAAC CCCTCCACCA TCAGCAGCAG
    CTTCTGCCCC CAAATGCTGT TATACCACAA GATATCCACC AACAAACTTG TCAGCCTCAA
    CCAGTGCTCC AGCATGTGCT CCCATTACAC ACTGCACCCA GTCAGGTACC TCAGTCCCAA
    GCTAGTACAG TACCTCTCTA CAGTCAGGTG GTGTCAGCTT CCCCCCAGCA GAAATTAACT
    CTTCACACTC AAACACAGTC TAGCATGCAG TCACAAGTGC TCTCAAACTC ACAGGCACAG
    ACTCACATAG AGCCCACTGG AGCATTTGAG CATCCTCATT ACACCCAGGC TGCCTTTATG
    CCATCACAGA TTCCCACAAG TCCATCTCAT ACTTCCATAC AGCCCTCTTC CACTCTCCCA
    GCCCAACAAC CAGTTGTCCC AGTTTCAGAA ATTCCTGTCC CAGCAGACGC ACAGCTAACA
    CAGGCAAGGC CTGCTGATTT GATCCCTGCT TCCACTCCTA CTGTCAATAC TACCCAGTCC
    CATGACTCAA ACGGCTCTGT GCTGACAACC GCCATGCTGG CAGAAAGTGA CGCTGCATTG
    CTGGGCATTG CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGCC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACACTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TCTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC GCACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTAACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCAGATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GACGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATCGCAACA GCAACCAATG
    CAACAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATCCAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGAACAT GCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TGTGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGCTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGG CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACC GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCAAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GATGGCAACT TTAGCCAGGG GATGAAGGAG GCATCTACAG ATGACCAACC CTGCTCACGC
    AGAGGCCGTT TCCAGGTGAT TCCAGTATCC CAGTCTGCAG AGACCCTTCC AACGCAGGAA
    GCTCCACCAA GCCAAATGAT GAGAGTTGGG CGTTTCTCAG TCACTCAACC TGAACATCGA
    GAGGAGCATT TGACCGACAG CTCCCCTGTG TCACCTGACC TGGAGCGAGA GAGGAGGAAA
    ACGAAAGACG AGAGGAGAAC GACAGCACCC AGCCACCCAT CCAAGAGCCA CGCGCACTCA
    CCCCTGGGCA GCAGTGATGA TGAGAGCGAG GTGGAGGATG AAGACTTAAG GAAAGAACTG
    CACAAACTCA GAGAGAAACA CATCAAGGAG GTGGTGTCCC TGCAGGCACA GCAGAACAGA
    GAACTACAGG ATTTGTACAA GCAGCTCCGC TCTCTCAAAG ACCACAGACA GCCAATGTCT
    ATGAGCCTGT CCCGCACCTC TCCTCTGCCC TCCGCACCCC TCGCTATCTC CCCGCGCAGA
    CCCCGACCCG CCAAAGCCAA GCTCCGGCCT CGGCCGCACT CTCACATGGA TAACAACGGA
    GTCGCACACC AAGGGAGTCT TCAGCAGTCT AGCAGTTATT CCGGTGGGGA GCAAAGCCGG
    CTACATTCAC ACTGCAAGCC AGAGAAATCA CAATCCCTGA CATCTAACAG AGACCACAGT
    CCTTCAGCCC AGGGTTCTGG CAACAGAAAG AGCACATTCA CAGATGAGCT GCATAAACTT
    GTCGATGAGT GGTCAAAGGA AACTGTTTGT CCAGTTCAGC CCAAGCCTTC GCTGAATCAG
    ATTAAACAGA TCCAACAAGT GCAGGAGTTG GGTGGATGGA GCCAGCCTGC TGAGACAACA
    GCTTCGTCGG GTTGGTTTTC CGGGGCCCCT TTAAGTACGC CTGTATCAGT CAGCATGACC
    ACCATGACAT CCACCCCCTA TAGCGCCATT AGTGGACAAA CACAGCCGCC TCCGACGCAA
    TTCCCTATCG TTCCTCTACC CCAGCAGAAC GTGCATCCAC AACCCGGCCC GCACCGTCAG
    CCCCTGCAGT ACGGCCCTTC ACCCCTGCAG CAGGCCCAGC TCCCGGTGCC TGCAGCAGGA
    CAAACACCGC CCCCAACCAA TCCAGACGCT GTATTCTGCT CATGCTCTTC CTCCTCCTGC
    TCCTCATCCT CCTGTTCCAC CTCCAGTGCC AAACTTCACC CCAAACCCTC CACGTCCACC
    CTTCCTTTAG GACAGCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146286
    Clone ID Related Accession (Same CDS sequence) XM_009296927.3
    Accession Version XM_009296927.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8976bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395152.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    CCCCTTCCAC CTTACTCAGC AGATGTTGCA TCCTCACAAA TGTTACACTC CCGGCCTACT
    GATGCCTCCA CTCCCCACAG TTCCCTAATA CAGTCACAGA CATACATGCC CCCAGTATCG
    CCCCAGGCAC CCATGGCTGT CCTTACGTCC TCTATTCCGA CTGGAGAACC AATAGCACCA
    GCAGGCAACA TCATGCCACC CATACAGACC CAAACTAGTA TTCCTCCTCT TCAACCCATG
    GATGTTTTGC CCCTGCAAAC TGTTGTTCAA AATCAACAGC AAGTGATGCC TGAATTGCCC
    AGAACGCTGC AACCTAACAT CCAGCAACCA ACACCACAAC AGCAGGCAAA TGTAATGCAG
    CAGCATCAGA CAGTCTTGTT GCCACAGCAA ACTGACCAGG TTCAGCAAAT TAAAGTTTTG
    TTACAACAGT CACAGCATGC AGAACAACCC CAGGTGGTTC TGCAGGAGCA GGCCCCACAT
    TCCAGTGCAT CTCAACAGCC ATATATCCCA TTACCACAGC AAGCACCGCC AACAATGACC
    AACCCACAGC AAAGTGAGCT GCAACAGCAG GTGTTTATAC AGCAACCTCA AAGCAAACCC
    CAACAACAAG TTGGGATACA GCCTCAAGTT CAGCAGCATC AGACATCAAT ACAGCAACCC
    TCAGTTGATC AGTATCAACA CCAGACTCAA CAACAGCAAG TGTATGTGCC ACAAATAGCA
    GATCCACAAG ACCAAAACGT GATAAAGACT GTGATACAAC AAGTACCGGT GCTGCAAGCA
    ATTCAGCAGC CATTACAATT GATAGAGCAA CAACAGCAGC AGCTTCTGAT TAGGCAACAA
    ATTGAGCAAC ATCAGAAGGC TGCTTTACAA AACCAAATGG AGCAACATCA ACAGCAAGTT
    TATATTCAGC CACCACAACA GCAAAAAGTG GAACAACAAC AAATTCTTAT GCAGCAACAG
    CAAAACTTGG CAGTAAAGCA ACAGATAGAG CAACAGCAGC ATGCATTAAA GCAACAAAAG
    CAAATAGAGC AGCAGCAAAA AGCCATTTTA CAGCAACAGG CAGAACAGCA AAGACAGCAG
    CAACAGACTT TGTTTCAGCA ACAGCAGCTG GAGCAGCAAC AAGTATTACT GCAGCAGCAA
    CAGCGTGAAC AACAACAGGC TCTTCTCCAG CAGCAACAGT TGGAGCAGCA AGCTTTACTG
    CAACAACAGC AGCAACTTCA GTTAAAGCAG CAACAAGCTC TTCTACATCA ACAACAGCTG
    GAGCAGCAGC AGCAGGCTTT GTTTAAACAG CAGCAGCTGG AGCAACAGCA AGCTCTTTTG
    CAGCAGCAGC AGCAGCAAGC TCTCGTTCAG CAGCAGCAAC AGTTAGAGCA AAAAGTACTG
    CTACAGCTGC AACAGCAACA GCAGCAACAC ATCGATCAGC AGCAAAATTT AATACGCCAA
    CAGCAGCCTC AGCAGATCGA GGCTGTTTTA CAGCAACAAC CACAACTTCA GAACTCAACT
    GATCAACAAT TGCAGCAGCA AATATTTCAA CAACAGACCG AGCAGCAACA GATGCAGCAG
    CATCTACTGA TGAAACAACA GCAGATGGAA TTACAGCGAC AACAAGCTCT ACAAGCTGAG
    AAACAACGAC AGCAAGTTCT ATTGCAACAA CAACATTTGG AGCAACATGC AGCTCTGGTG
    GATCATAGTG GGCAGCAACA AGCAACCCTG GTGAAAGCAC AGTCTCCAGA AATGGTCCAG
    CAGATTAGTC AAGTGCAGCA ATCTCAACAA ATGATGTCTC TACAACCGCA GCCTCATCTT
    GCTCAACAAC AAACTGACCA GACACAGGTC TTGACTCAGC AACAAGCCAT TCTTCCCCAA
    GCAACACACA GACCTTCTCT GGTAGACTTG CAAATCCCAA CCCTGCTTCA AGCAGCACCT
    AAAATAGTCA GCACTCAAAT ACCTACCCAA GTTCAAGCCC CAGTACTTTT GCAGCAACAG
    GTGCCACCTC AGATGTCAGC TCAACCTACA GCACAAGTAC AACTCCAGAG TCAAGGTGAA
    CCAACAATTT ATAACCAGAT TGCAATGCAG AGTCAAAGTC ATCCCGCACA GCAGTCAATG
    GGAGGTCAAA TTCAGACAGG CCAATTGCAA ATTCCACAAT CAACCCAACA AATCACATCC
    CCAACACAAG TTCCTACACC ACAACCCATT CATTCTTTTG TTCCAACTTC AACACAGATG
    GTCTCACAAG GGCAAACCCT TCCACCTCAA CCTCAACTGG TACCCCCTCA AGCTGCAGCT
    TCTCTCCAGT GTCAAGTTGC AGTAGGGTCA TCTGCACCTG CATCTTTTGT ACAAGCTACA
    GTCCCAACTG CTTCTACACT TGTGCCCACA CAGTATGCTC AAGCACCTGT TGCTTCTCAA
    ACGATCCAGT CTGTTCAGTT AGACCTCACT CAACCTGTGA AGCAGCAACA ACAACAACAG
    CCGCCTCTGC AGCAATATCA GCAAGCGGTG CTATCTCCGG TGTCACTTGC ACCTACGATC
    AATCCAGTGG CTTCTTCCTC AACACAGTGC TATTTACAGC AGACAAACCA ATCACAAATG
    CAGGTCCAAA CCCAACAAGT AATTCAAGCT CAGCAACAAC CAGTCAGTTT GGCCTCGATG
    CAGGCAGTCT TGCAGCCCCT GCCTTTAGTG CAGCCTCAGG CTACCCAACA GTATGAGCAG
    CATTTGCCTC AAGCCCACCA TGTGCAACAA CAACCCCTCC ACCATCAGCA GCAGCTTCTG
    CCCCCAAATG CTGTTATACC ACAAGATATC CACCAACAAA CTTGTCAGCC TCAACCAGTG
    CTCCAGCATG TGCTCCCATT ACACACTGCA CCCAGTCAGG TACCTCAGTC CCAAGCTAGT
    ACAGTACCTC TCTACAGTCA GGTGGTGTCA GCTTCCCCCC AGCAGAAATT AACTCTTCAC
    ACTCAAACAC AGTCTAGCAT GCAGTCACAA GTGCTCTCAA ACTCACAGGC ACAGACTCAC
    ATAGAGCCCA CTGGAGCATT TGAGCATCCT CATTACACCC AGGCTGCCTT TATGCCATCA
    CAGATTCCCA CAAGTCCATC TCATACTTCC ATACAGCCCT CTTCCACTCT CCCAGCCCAA
    CAACCAGTTG TCCCAGTTTC AGAAATTCCT GTCCCAGCAG ACGCACAGCT AACACAGGCA
    AGGCCTGCTG ATTTGATCCC TGCTTCCACT CCTACTGTCA ATACTACCCA GTCCCATGAC
    TCAAACGGCT CTGTGCTGAC AACCGCCATG CTGGCAGAAA GTGACGCTGC ATTGCTGGGC
    ATTGCTCAGG AAAGCCTAAT TCACTCTGCC AACAGGGGAT CTTTATTATG CTCTGCTCCT
    ACCAATGGAG ATGAGGGCCA GTTGGTGTGG CCTAGTGGAA AACTAGATAA GGCAAAATGT
    CAGAGGAGAT CATCCTGTCA GAAGACTGAA AAAACTCACT TCCAACTCTG CATGTTGCAG
    GTCTCATGCA CAGGAGACAA CATGGTAGAA TGCCAGTTGG AGACTCACAG CAACAAGATG
    GTCACGTTCA AGTTTGACAC TGAGGGAGAT GCACCTGAAG ACATTGCAGA TTACATGGTG
    GAGGAGGACT TCGTCCTTGA GCCTGAAAAA GAAAAATTTG TTGAGGAGCT GAGATCTATA
    GTGAAGAAAG CTCAAGCAAT ATTAAGTACT CAGTCGCAGA CTGGATCCCT GGAGCAGCTC
    CAAGTGAGCA CCCCATCCAG TGCAACAATG GATTCAGCCC CTCAGTCTTC TCCAGTAGGT
    CGCTGGCGGT TCTTCATTAA CCAGACTATT CGTCACCGTG ATTCGCACTC CAACCAGGGA
    GCTTCGACTC CCCCTCCAGT GGGGGAGAAC AGAGTTCCCA AGTCCATAGA AACCGAGAAA
    GATCATGAGA GCCCGCAGCG CTCCGAGTCT TTTGCTGGAA TAGCATCTTC CCCAAGTTCT
    TCACTATCTG CATCCTCTCC TCCAGTCTCT ACTGTATCCG CTCCTGCATC CATGTCAGCC
    TCAGCCATGG CTGCTCAAAA AGATTTAACA CCTGCTGCCT TGCCAGCAGT AGAGAAAGTC
    TCCTCTACTA TGCTGACCGC AGGTCCAGTC CCTGTCATGG CCTCCAGCAC TTCTGCCACT
    CCTTTTCCTG CAGCCATGGC CCTCACAGGC TCTAATGTGG ACCAAGGAGA ATCTGCTGTT
    AAAGGTGAGG GTCAACAGCC TTATGTGACT CCTCCAACGT CACTGTTGAC CCATGCTGAC
    CCATCTCAGC ACTTAATTGT TCAAACCCAG ATGGCACCCT CACAGATGCC AGATATACCT
    CAACAAAGCC AGACACTTCA ATCAACATTG ATCACAACTG AACAAGTTCA GCAACCGCAG
    ATTACCCCTC AGCCACTTCT GCAGGACGCT CCACAGCTAC CTAAACCAGA ATCAAAATCG
    CTACCACAGG CAGTGCCTAA AGAGATGGTT CCTTTGCATG AAGGTCTTTC AGCTGCCCAA
    GTTCAGCAGG TCCCTCTCCA GCAAGTACCA GTAGAATCGC AACAGCAACC AATGCAACAG
    ACCACATTCT TACAGCAAAC ATCCAGTGCA GAGCAGATCC AGCAGCACAT GGCAGAACAG
    TTTTTATCTC AAGAAACCAA ATCAGCTCCA ATGATACTGA ACATGCATCC ACAGCATGGC
    TTCCAGACCC AGGATCCTCA GCAGGTTCAA GCTCCATTGC AGTCTGTGAT CCCCCAGCAG
    GCTCCAATTG AGCAAACCCA GACATTATAT AAACAGCTCC CACATGATAT GATGCCAGTG
    GCAAAAACCG CAGTGCAGAA GCAAGTGTCT TTCCAGCAGT CAGAGTCTGA GCTGTCTACG
    GGTGAGGACA CCGGCAGCCA CTCTGCACTT CCGCATCCCT CTTTAGACAC CTCTCTTCCA
    CCCCTCCCTC TCTCTGAAAC AGCTCTTCCT GCCCTCTCCC ACACATTCAC TCCTTCTCCA
    GCACAGCCTT CTTCAGTAGC AGAGTCGGAC AGTGAAGGCC CTCCCAAAAT TGAGTTTGTG
    GATAACCGAA TCAAGACTTT GGATGAGAAA TTGAGGACTC TGCTCTATCA GGAGCACGGC
    GGCAGTGGAG CAGCTGTGGG CAGTGCCTCC ACAGGAGGAA CTGCTCCAGC CTGCTCCACT
    TCAGGATCTG CCACAGCCTC TACCGCAGTT GATGAGTCTT CAGAAGTCCC CTACGCATTC
    TCCCTACCTC CTGCCACCTC CTCGGACACC TCCCCTCACT CCACCTCTTC CACAACCTCC
    TCCACCACCC CTCGCTCTTC CTCCACCTCC CCGGATGGAG AGCGAGAGAA AGCAGGCGAG
    GAGACGACGA CTATCCCACC TGCGGTACAA CAACAGCCTG CCTCCTCTAC ATCCCCTCCA
    TGCTCTCTTC TTTCCCATCC ACAGGAGCCT GGATCCCCAA AAGTTACTAT TCAATCATCA
    GTAAGCGCTC CCCCTTCCCT CTCTGACAGC TGCACCCCTG GAGATGTTCT GTTGCCTTCC
    TCTTCTCAGC CGCCCGTCCC ACTGTGGCCT GGACAGCAGC AGCACAATGA AGGAGGTGGA
    TATTTTGGCC TAAACCTGAC ATGTCCTAGT ATCAGAAATC CTGTTAGCAA GAAATCCTGG
    ACTCGCAAAT TCAAAAGCTG GGCATGCAAA CTGCGCCACT CCGCCAGCTT GTTCAAGAAG
    CCCAGAGTCC AGCAAGATGG CAACTTTAGC CAGGGGATGA AGGAGGCATC TACAGATGAC
    CAACCCTGCT CACGCAGAGG CCGTTTCCAG GTGATTCCAG TATCCCAGTC TGCAGAGACC
    CTTCCAACGC AGGAAGCTCC ACCAAGCCAA ATGATGAGAG TTGGGCGTTT CTCAGTCACT
    CAACCTGAAC ATCGAGAGGA GCATTTGACC GACAGCTCCC CTGTGTCACC TGACCTGGAG
    CGAGAGAGGA GGAAAACGAA AGACGAGAGG AGAACGACAG CACCCAGCCA CCCATCCAAG
    AGCCACGCGC ACTCACCCCT GGGCAGCAGT GATGATGAGA GCGAGGTGGA GGATGAAGAC
    TTAAGGAAAG AACTGCACAA ACTCAGAGAG AAACACATCA AGGAGGTGGT GTCCCTGCAG
    GCACAGCAGA ACAGAGAACT ACAGGATTTG TACAAGCAGC TCCGCTCTCT CAAAGACCAC
    AGACAGCCAA TGTCTATGAG CCTGTCCCGC ACCTCTCCTC TGCCCTCCGC ACCCCTCGCT
    ATCTCCCCGC GCAGACCCCG ACCCGCCAAA GCCAAGCTCC GGCCTCGGCC GCACTCTCAC
    ATGGATAACA ACGGAGTCGC ACACCAAGGG AGTCTTCAGC AGTCTAGCAG TTATTCCGGT
    GGGGAGCAAA GCCGGCTACA TTCACACTGC AAGCCAGAGA AATCACAATC CCTGACATCT
    AACAGAGACC ACAGTCCTTC AGCCCAGGGT TCTGGCAACA GAAAGAGCAC ATTCACAGAT
    GAGCTGCATA AACTTGTCGA TGAGTGGTCA AAGGAAACTG TTTGTCCAGT TCAGCCCAAG
    CCTTCGCTGA ATCAGATTAA ACAGATCCAA CAAGTGCAGG AGTTGGGTGG ATGGAGCCAG
    CCTGCTGAGA CAACAGCTTC GTCGGGTTGG TTTTCCGGGG CCCCTTTAAG TACGCCTGTA
    TCAGTCAGCA TGACCACCAT GACATCCACC CCCTATAGCG CCATTAGTGG ACAAACACAG
    CCGCCTCCGA CGCAATTCCC TATCGTTCCT CTACCCCAGC AGAACGTGCA TCCACAACCC
    GGCCCGCACC GTCAGCCCCT GCAGTACGGC CCTTCACCCC TGCAGCAGGC CCAGCTCCCG
    GTGCCTGCAG CAGGACAAAC ACCGCCCCCA ACCAATCCAG ACGCTGTATT CTGCTCATGC
    TCTTCCTCCT CCTGCTCCTC ATCCTCCTGT TCCACCTCCA GTGCCAAACT TCACCCCAAA
    CCCTCCACGT CCACCCTTCC TTTAGGACAG CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295202.2
    CDS1064..10039
    Translation

    Target ORF information:

    RefSeq Version XM_009296927.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296927.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    CCCCTTCCAC CTTACTCAGC AGATGTTGCA TCCTCACAAA TGTTACACTC CCGGCCTACT
    GATGCCTCCA CTCCCCACAG TTCCCTAATA CAGTCACAGA CATACATGCC CCCAGTATCG
    CCCCAGGCAC CCATGGCTGT CCTTACGTCC TCTATTCCGA CTGGAGAACC AATAGCACCA
    GCAGGCAACA TCATGCCACC CATACAGACC CAAACTAGTA TTCCTCCTCT TCAACCCATG
    GATGTTTTGC CCCTGCAAAC TGTTGTTCAA AATCAACAGC AAGTGATGCC TGAATTGCCC
    AGAACGCTGC AACCTAACAT CCAGCAACCA ACACCACAAC AGCAGGCAAA TGTAATGCAG
    CAGCATCAGA CAGTCTTGTT GCCACAGCAA ACTGACCAGG TTCAGCAAAT TAAAGTTTTG
    TTACAACAGT CACAGCATGC AGAACAACCC CAGGTGGTTC TGCAGGAGCA GGCCCCACAT
    TCCAGTGCAT CTCAACAGCC ATATATCCCA TTACCACAGC AAGCACCGCC AACAATGACC
    AACCCACAGC AAAGTGAGCT GCAACAGCAG GTGTTTATAC AGCAACCTCA AAGCAAACCC
    CAACAACAAG TTGGGATACA GCCTCAAGTT CAGCAGCATC AGACATCAAT ACAGCAACCC
    TCAGTTGATC AGTATCAACA CCAGACTCAA CAACAGCAAG TGTATGTGCC ACAAATAGCA
    GATCCACAAG ACCAAAACGT GATAAAGACT GTGATACAAC AAGTACCGGT GCTGCAAGCA
    ATTCAGCAGC CATTACAATT GATAGAGCAA CAACAGCAGC AGCTTCTGAT TAGGCAACAA
    ATTGAGCAAC ATCAGAAGGC TGCTTTACAA AACCAAATGG AGCAACATCA ACAGCAAGTT
    TATATTCAGC CACCACAACA GCAAAAAGTG GAACAACAAC AAATTCTTAT GCAGCAACAG
    CAAAACTTGG CAGTAAAGCA ACAGATAGAG CAACAGCAGC ATGCATTAAA GCAACAAAAG
    CAAATAGAGC AGCAGCAAAA AGCCATTTTA CAGCAACAGG CAGAACAGCA AAGACAGCAG
    CAACAGACTT TGTTTCAGCA ACAGCAGCTG GAGCAGCAAC AAGTATTACT GCAGCAGCAA
    CAGCGTGAAC AACAACAGGC TCTTCTCCAG CAGCAACAGT TGGAGCAGCA AGCTTTACTG
    CAACAACAGC AGCAACTTCA GTTAAAGCAG CAACAAGCTC TTCTACATCA ACAACAGCTG
    GAGCAGCAGC AGCAGGCTTT GTTTAAACAG CAGCAGCTGG AGCAACAGCA AGCTCTTTTG
    CAGCAGCAGC AGCAGCAAGC TCTCGTTCAG CAGCAGCAAC AGTTAGAGCA AAAAGTACTG
    CTACAGCTGC AACAGCAACA GCAGCAACAC ATCGATCAGC AGCAAAATTT AATACGCCAA
    CAGCAGCCTC AGCAGATCGA GGCTGTTTTA CAGCAACAAC CACAACTTCA GAACTCAACT
    GATCAACAAT TGCAGCAGCA AATATTTCAA CAACAGACCG AGCAGCAACA GATGCAGCAG
    CATCTACTGA TGAAACAACA GCAGATGGAA TTACAGCGAC AACAAGCTCT ACAAGCTGAG
    AAACAACGAC AGCAAGTTCT ATTGCAACAA CAACATTTGG AGCAACATGC AGCTCTGGTG
    GATCATAGTG GGCAGCAACA AGCAACCCTG GTGAAAGCAC AGTCTCCAGA AATGGTCCAG
    CAGATTAGTC AAGTGCAGCA ATCTCAACAA ATGATGTCTC TACAACCGCA GCCTCATCTT
    GCTCAACAAC AAACTGACCA GACACAGGTC TTGACTCAGC AACAAGCCAT TCTTCCCCAA
    GCAACACACA GACCTTCTCT GGTAGACTTG CAAATCCCAA CCCTGCTTCA AGCAGCACCT
    AAAATAGTCA GCACTCAAAT ACCTACCCAA GTTCAAGCCC CAGTACTTTT GCAGCAACAG
    GTGCCACCTC AGATGTCAGC TCAACCTACA GCACAAGTAC AACTCCAGAG TCAAGGTGAA
    CCAACAATTT ATAACCAGAT TGCAATGCAG AGTCAAAGTC ATCCCGCACA GCAGTCAATG
    GGAGGTCAAA TTCAGACAGG CCAATTGCAA ATTCCACAAT CAACCCAACA AATCACATCC
    CCAACACAAG TTCCTACACC ACAACCCATT CATTCTTTTG TTCCAACTTC AACACAGATG
    GTCTCACAAG GGCAAACCCT TCCACCTCAA CCTCAACTGG TACCCCCTCA AGCTGCAGCT
    TCTCTCCAGT GTCAAGTTGC AGTAGGGTCA TCTGCACCTG CATCTTTTGT ACAAGCTACA
    GTCCCAACTG CTTCTACACT TGTGCCCACA CAGTATGCTC AAGCACCTGT TGCTTCTCAA
    ACGATCCAGT CTGTTCAGTT AGACCTCACT CAACCTGTGA AGCAGCAACA ACAACAACAG
    CCGCCTCTGC AGCAATATCA GCAAGCGGTG CTATCTCCGG TGTCACTTGC ACCTACGATC
    AATCCAGTGG CTTCTTCCTC AACACAGTGC TATTTACAGC AGACAAACCA ATCACAAATG
    CAGGTCCAAA CCCAACAAGT AATTCAAGCT CAGCAACAAC CAGTCAGTTT GGCCTCGATG
    CAGGCAGTCT TGCAGCCCCT GCCTTTAGTG CAGCCTCAGG CTACCCAACA GTATGAGCAG
    CATTTGCCTC AAGCCCACCA TGTGCAACAA CAACCCCTCC ACCATCAGCA GCAGCTTCTG
    CCCCCAAATG CTGTTATACC ACAAGATATC CACCAACAAA CTTGTCAGCC TCAACCAGTG
    CTCCAGCATG TGCTCCCATT ACACACTGCA CCCAGTCAGG TACCTCAGTC CCAAGCTAGT
    ACAGTACCTC TCTACAGTCA GGTGGTGTCA GCTTCCCCCC AGCAGAAATT AACTCTTCAC
    ACTCAAACAC AGTCTAGCAT GCAGTCACAA GTGCTCTCAA ACTCACAGGC ACAGACTCAC
    ATAGAGCCCA CTGGAGCATT TGAGCATCCT CATTACACCC AGGCTGCCTT TATGCCATCA
    CAGATTCCCA CAAGTCCATC TCATACTTCC ATACAGCCCT CTTCCACTCT CCCAGCCCAA
    CAACCAGTTG TCCCAGTTTC AGAAATTCCT GTCCCAGCAG ACGCACAGCT AACACAGGCA
    AGGCCTGCTG ATTTGATCCC TGCTTCCACT CCTACTGTCA ATACTACCCA GTCCCATGAC
    TCAAACGGCT CTGTGCTGAC AACCGCCATG CTGGCAGAAA GTGACGCTGC ATTGCTGGGC
    ATTGCTCAGG AAAGCCTAAT TCACTCTGCC AACAGGGGAT CTTTATTATG CTCTGCTCCT
    ACCAATGGAG ATGAGGGCCA GTTGGTGTGG CCTAGTGGAA AACTAGATAA GGCAAAATGT
    CAGAGGAGAT CATCCTGTCA GAAGACTGAA AAAACTCACT TCCAACTCTG CATGTTGCAG
    GTCTCATGCA CAGGAGACAA CATGGTAGAA TGCCAGTTGG AGACTCACAG CAACAAGATG
    GTCACGTTCA AGTTTGACAC TGAGGGAGAT GCACCTGAAG ACATTGCAGA TTACATGGTG
    GAGGAGGACT TCGTCCTTGA GCCTGAAAAA GAAAAATTTG TTGAGGAGCT GAGATCTATA
    GTGAAGAAAG CTCAAGCAAT ATTAAGTACT CAGTCGCAGA CTGGATCCCT GGAGCAGCTC
    CAAGTGAGCA CCCCATCCAG TGCAACAATG GATTCAGCCC CTCAGTCTTC TCCAGTAGGT
    CGCTGGCGGT TCTTCATTAA CCAGACTATT CGTCACCGTG ATTCGCACTC CAACCAGGGA
    GCTTCGACTC CCCCTCCAGT GGGGGAGAAC AGAGTTCCCA AGTCCATAGA AACCGAGAAA
    GATCATGAGA GCCCGCAGCG CTCCGAGTCT TTTGCTGGAA TAGCATCTTC CCCAAGTTCT
    TCACTATCTG CATCCTCTCC TCCAGTCTCT ACTGTATCCG CTCCTGCATC CATGTCAGCC
    TCAGCCATGG CTGCTCAAAA AGATTTAACA CCTGCTGCCT TGCCAGCAGT AGAGAAAGTC
    TCCTCTACTA TGCTGACCGC AGGTCCAGTC CCTGTCATGG CCTCCAGCAC TTCTGCCACT
    CCTTTTCCTG CAGCCATGGC CCTCACAGGC TCTAATGTGG ACCAAGGAGA ATCTGCTGTT
    AAAGGTGAGG GTCAACAGCC TTATGTGACT CCTCCAACGT CACTGTTGAC CCATGCTGAC
    CCATCTCAGC ACTTAATTGT TCAAACCCAG ATGGCACCCT CACAGATGCC AGATATACCT
    CAACAAAGCC AGACACTTCA ATCAACATTG ATCACAACTG AACAAGTTCA GCAACCGCAG
    ATTACCCCTC AGCCACTTCT GCAGGACGCT CCACAGCTAC CTAAACCAGA ATCAAAATCG
    CTACCACAGG CAGTGCCTAA AGAGATGGTT CCTTTGCATG AAGGTCTTTC AGCTGCCCAA
    GTTCAGCAGG TCCCTCTCCA GCAAGTACCA GTAGAATCGC AACAGCAACC AATGCAACAG
    ACCACATTCT TACAGCAAAC ATCCAGTGCA GAGCAGATCC AGCAGCACAT GGCAGAACAG
    TTTTTATCTC AAGAAACCAA ATCAGCTCCA ATGATACTGA ACATGCATCC ACAGCATGGC
    TTCCAGACCC AGGATCCTCA GCAGGTTCAA GCTCCATTGC AGTCTGTGAT CCCCCAGCAG
    GCTCCAATTG AGCAAACCCA GACATTATAT AAACAGCTCC CACATGATAT GATGCCAGTG
    GCAAAAACCG CAGTGCAGAA GCAAGTGTCT TTCCAGCAGT CAGAGTCTGA GCTGTCTACG
    GGTGAGGACA CCGGCAGCCA CTCTGCACTT CCGCATCCCT CTTTAGACAC CTCTCTTCCA
    CCCCTCCCTC TCTCTGAAAC AGCTCTTCCT GCCCTCTCCC ACACATTCAC TCCTTCTCCA
    GCACAGCCTT CTTCAGTAGC AGAGTCGGAC AGTGAAGGCC CTCCCAAAAT TGAGTTTGTG
    GATAACCGAA TCAAGACTTT GGATGAGAAA TTGAGGACTC TGCTCTATCA GGAGCACGGC
    GGCAGTGGAG CAGCTGTGGG CAGTGCCTCC ACAGGAGGAA CTGCTCCAGC CTGCTCCACT
    TCAGGATCTG CCACAGCCTC TACCGCAGTT GATGAGTCTT CAGAAGTCCC CTACGCATTC
    TCCCTACCTC CTGCCACCTC CTCGGACACC TCCCCTCACT CCACCTCTTC CACAACCTCC
    TCCACCACCC CTCGCTCTTC CTCCACCTCC CCGGATGGAG AGCGAGAGAA AGCAGGCGAG
    GAGACGACGA CTATCCCACC TGCGGTACAA CAACAGCCTG CCTCCTCTAC ATCCCCTCCA
    TGCTCTCTTC TTTCCCATCC ACAGGAGCCT GGATCCCCAA AAGTTACTAT TCAATCATCA
    GTAAGCGCTC CCCCTTCCCT CTCTGACAGC TGCACCCCTG GAGATGTTCT GTTGCCTTCC
    TCTTCTCAGC CGCCCGTCCC ACTGTGGCCT GGACAGCAGC AGCACAATGA AGGAGGTGGA
    TATTTTGGCC TAAACCTGAC ATGTCCTAGT ATCAGAAATC CTGTTAGCAA GAAATCCTGG
    ACTCGCAAAT TCAAAAGCTG GGCATGCAAA CTGCGCCACT CCGCCAGCTT GTTCAAGAAG
    CCCAGAGTCC AGCAAGATGG CAACTTTAGC CAGGGGATGA AGGAGGCATC TACAGATGAC
    CAACCCTGCT CACGCAGAGG CCGTTTCCAG GTGATTCCAG TATCCCAGTC TGCAGAGACC
    CTTCCAACGC AGGAAGCTCC ACCAAGCCAA ATGATGAGAG TTGGGCGTTT CTCAGTCACT
    CAACCTGAAC ATCGAGAGGA GCATTTGACC GACAGCTCCC CTGTGTCACC TGACCTGGAG
    CGAGAGAGGA GGAAAACGAA AGACGAGAGG AGAACGACAG CACCCAGCCA CCCATCCAAG
    AGCCACGCGC ACTCACCCCT GGGCAGCAGT GATGATGAGA GCGAGGTGGA GGATGAAGAC
    TTAAGGAAAG AACTGCACAA ACTCAGAGAG AAACACATCA AGGAGGTGGT GTCCCTGCAG
    GCACAGCAGA ACAGAGAACT ACAGGATTTG TACAAGCAGC TCCGCTCTCT CAAAGACCAC
    AGACAGCCAA TGTCTATGAG CCTGTCCCGC ACCTCTCCTC TGCCCTCCGC ACCCCTCGCT
    ATCTCCCCGC GCAGACCCCG ACCCGCCAAA GCCAAGCTCC GGCCTCGGCC GCACTCTCAC
    ATGGATAACA ACGGAGTCGC ACACCAAGGG AGTCTTCAGC AGTCTAGCAG TTATTCCGGT
    GGGGAGCAAA GCCGGCTACA TTCACACTGC AAGCCAGAGA AATCACAATC CCTGACATCT
    AACAGAGACC ACAGTCCTTC AGCCCAGGGT TCTGGCAACA GAAAGAGCAC ATTCACAGAT
    GAGCTGCATA AACTTGTCGA TGAGTGGTCA AAGGAAACTG TTTGTCCAGT TCAGCCCAAG
    CCTTCGCTGA ATCAGATTAA ACAGATCCAA CAAGTGCAGG AGTTGGGTGG ATGGAGCCAG
    CCTGCTGAGA CAACAGCTTC GTCGGGTTGG TTTTCCGGGG CCCCTTTAAG TACGCCTGTA
    TCAGTCAGCA TGACCACCAT GACATCCACC CCCTATAGCG CCATTAGTGG ACAAACACAG
    CCGCCTCCGA CGCAATTCCC TATCGTTCCT CTACCCCAGC AGAACGTGCA TCCACAACCC
    GGCCCGCACC GTCAGCCCCT GCAGTACGGC CCTTCACCCC TGCAGCAGGC CCAGCTCCCG
    GTGCCTGCAG CAGGACAAAC ACCGCCCCCA ACCAATCCAG ACGCTGTATT CTGCTCATGC
    TCTTCCTCCT CCTGCTCCTC ATCCTCCTGT TCCACCTCCA GTGCCAAACT TCACCCCAAA
    CCCTCCACGT CCACCCTTCC TTTAGGACAG CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146287
    Clone ID Related Accession (Same CDS sequence) XM_009296928.3
    Accession Version XM_009296928.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395152.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296928.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
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    4921
    4981
    5041
    5101
    5161
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    5281
    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGCCCC TTCCACCTTA CTCAGCAGAT GTTGCATCCT CACAAATGTT ACACTCCCGG
    CCTACTGATG CCTCCACTCC CCACAGTTCC CTAATACAGT CACAGACATA CATGCCCCCA
    GTATCGCCCC AGGCACCCAT GGCTGTCCTT ACGTCCTCTA TTCCGACTGG AGAACCAATA
    GCACCAGCAG GCAACATCAT GCCACCCATA CAGACCCAAA CTAGTATTCC TCCTCTTCAA
    CCCATGGATG TTTTGCCCCT GCAAACTGTT GTTCAAAATC AACAGCAAGT GATGCCTGAA
    TTGCCCAGAA CGCTGCAACC TAACATCCAG CAACCAACAC CACAACAGCA GGCAAATGTA
    ATGCAGCAGC ATCAGACAGT CTTGTTGCCA CAGCAAACTG ACCAGGTTCA GCAAATTAAA
    GTTTTGTTAC AACAGTCACA GCATGCAGAA CAACCCCAGG TGGTTCTGCA GGAGCAGGCC
    CCACATTCCA GTGCATCTCA ACAGCCATAT ATCCCATTAC CACAGCAAGC ACCGCCAACA
    ATGACCAACC CACAGCAAAG TGAGCTGCAA CAGCAGGTGT TTATACAGCA ACCTCAAAGC
    AAACCCCAAC AACAAGTTGG GATACAGCCT CAAGTTCAGC AGCATCAGAC ATCAATACAG
    CAACCCTCAG TTGATCAGTA TCAACACCAG ACTCAACAAC AGCAAGTGTA TGTGCCACAA
    ATAGCAGATC CACAAGACCA AAACGTGATA AAGACTGTGA TACAACAAGT ACCGGTGCTG
    CAAGCAATTC AGCAGCCATT ACAATTGATA GAGCAACAAC AGCAGCAGCT TCTGATTAGG
    CAACAAATTG AGCAACATCA GAAGGCTGCT TTACAAAACC AAATGGAGCA ACATCAACAG
    CAAGTTTATA TTCAGCCACC ACAACAGCAA AAAGTGGAAC AACAACAAAT TCTTATGCAG
    CAACAGCAAA ACTTGGCAGT AAAGCAACAG ATAGAGCAAC AGCAGCATGC ATTAAAGCAA
    CAAAAGCAAA TAGAGCAGCA GCAAAAAGCC ATTTTACAGC AACAGGCAGA ACAGCAAAGA
    CAGCAGCAAC AGACTTTGTT TCAGCAACAG CAGCTGGAGC AGCAACAAGT ATTACTGCAG
    CAGCAACAGC GTGAACAACA ACAGGCTCTT CTCCAGCAGC AACAGTTGGA GCAGCAAGCT
    TTACTGCAAC AACAGCAGCA ACTTCAGTTA AAGCAGCAAC AAGCTCTTCT ACATCAACAA
    CAGCTGGAGC AGCAGCAGCA GGCTTTGTTT AAACAGCAGC AGCTGGAGCA ACAGCAAGCT
    CTTTTGCAGC AGCAGCAGCA GCAAGCTCTC GTTCAGCAGC AGCAACAGTT AGAGCAAAAA
    GTACTGCTAC AGCTGCAACA GCAACAGCAG CAACACATCG ATCAGCAGCA AAATTTAATA
    CGCCAACAGC AGCCTCAGCA GATCGAGGCT GTTTTACAGC AACAACCACA ACTTCAGAAC
    TCAACTGATC AACAATTGCA GCAGCAAATA TTTCAACAAC AGACCGAGCA GCAACAGATG
    CAGCAGCATC TACTGATGAA ACAACAGCAG ATGGAATTAC AGCGACAACA AGCTCTACAA
    GCTGAGAAAC AACGACAGCA AGTTCTATTG CAACAACAAC ATTTGGAGCA ACATGCAGCT
    CTGGTGGATC ATAGTGGGCA GCAACAAGCA ACCCTGGTGA AAGCACAGTC TCCAGAAATG
    GTCCAGCAGA TTAGTCAAGT GCAGCAATCT CAACAAATGA TGTCTCTACA ACCGCAGCCT
    CATCTTGCTC AACAACAAAC TGACCAGACA CAGGTCTTGA CTCAGCAACA AGCCATTCTT
    CCCCAAGCAA CACACAGACC TTCTCTGGTA GACTTGCAAA TCCCAACCCT GCTTCAAGCA
    GCACCTAAAA TAGTCAGCAC TCAAATACCT ACCCAAGTTC AAGCCCCAGT ACTTTTGCAG
    CAACAGGTGC CACCTCAGAT GTCAGCTCAA CCTACAGCAC AAGTACAACT CCAGAGTCAA
    GGTGAACCAA CAATTTATAA CCAGATTGCA ATGCAGAGTC AAAGTCATCC CGCACAGCAG
    TCAATGGGAG GTCAAATTCA GACAGGCCAA TTGCAAATTC CACAATCAAC CCAACAAATC
    ACATCCCCAA CACAAGTTCC TACACCACAA CCCATTCATT CTTTTGTTCC AACTTCAACA
    CAGATGGTCT CACAAGGGCA AACCCTTCCA CCTCAACCTC AACTGGTACC CCCTCAAGCT
    GCAGCTTCTC TCCAGTGTCA AGTTGCAGTA GGGTCATCTG CACCTGCATC TTTTGTACAA
    GCTACAGTCC CAACTGCTTC TACACTTGTG CCCACACAGT ATGCTCAAGC ACCTGTTGCT
    TCTCAAACGA TCCAGTCTGT TCAGTTAGAC CTCACTCAAC CTGTGAAGCA GCAACAACAA
    CAACAGCCGC CTCTGCAGCA ATATCAGCAA GCGGTGCTAT CTCCGGTGTC ACTTGCACCT
    ACGATCAATC CAGTGGCTTC TTCCTCAACA CAGTGCTATT TACAGCAGAC AAACCAATCA
    CAAATGCAGG TCCAAACCCA ACAAGTAATT CAAGCTCAGC AACAACCAGT CAGTTTGGCC
    TCGATGCAGG CAGTCTTGCA GCCCCTGCCT TTAGTGCAGC CTCAGGCTAC CCAACAGTAT
    GAGCAGCATT TGCCTCAAGC CCACCATGTG CAACAACAAC CCCTCCACCA TCAGCAGCAG
    CTTCTGCCCC CAAATGCTGT TATACCACAA GATATCCACC AACAAACTTG TCAGCCTCAA
    CCAGTGCTCC AGCATGTGCT CCCATTACAC ACTGCACCCA GTCAGGTACC TCAGTCCCAA
    GCTAGTACAG TACCTCTCTA CAGTCAGGTG GTGTCAGCTT CCCCCCAGCA GAAATTAACT
    CTTCACACTC AAACACAGTC TAGCATGCAG TCACAAGTGC TCTCAAACTC ACAGGCACAG
    ACTCACATAG AGCCCACTGG AGCATTTGAG CATCCTCATT ACACCCAGGC TGCCTTTATG
    CCATCACAGA TTCCCACAAG TCCATCTCAT ACTTCCATAC AGCCCTCTTC CACTCTCCCA
    GCCCAACAAC CAGTTGTCCC AGTTTCAGAA ATTCCTGTCC CAGCAGACGC ACAGCTAACA
    CAGGCAAGGC CTGCTGATTT GATCCCTGCT TCCACTCCTA CTGTCAATAC TACCCAGTCC
    CATGACTCAA ACGGCTCTGT GCTGACAACC GCCATGCTGG CAGAAAGTGA CGCTGCATTG
    CTGGGCATTG CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGCC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACACTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TCTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC GCACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTAACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCAGATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GACGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATCGCAACA GCAACCAATG
    CAACAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATCCAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGAACAT GCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TGTGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGCTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGG CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACC GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCAAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGGGGTAAGA ACAGACGAGG GTAGAAAAGG GAGAGAATCT
    AAGAGGGATG ACCCTGGATC TGAGTCTCCA GTCTCACCGT ATGCAGTGCT GTTATTGCGG
    TGCCTGCACC CCTCACCATC CTGCACCCGC CTCACTCACC CACAGCACCT GAAATGGCTA
    AAATGGCACA CTTGTTTAAA AACTACATGG GCTGGACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295203.2
    CDS1064..9145
    Translation

    Target ORF information:

    RefSeq Version XM_009296928.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296928.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTAAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAA
    CAACAACAAA GTCTGGATGA ACGCCGTGAT TCTTCTGTCC TTACCACCTC CATTCCTTAT
    GTTCATCCTT CTTGCACCAC CTCCTCACAG GGCACAGGTG CCGCTGCAGC TGCCGGGGGT
    CAAGGAGAGG CAGAGGAGCT GCCAGAAGTT GACCAACATG TCCAACAACA AGGCACTGCA
    TCAGGCCTCA CAGAGAGTGA GAGTCTTGCT GCTGTCCCTG GACAGAGCCA AGCCTTTTCA
    GCTCCCGATT CAGGGAATCC TTGTGCTCAT GCTCAAACCA GCTACCCCAC TGGCACATCT
    GGAGCGTCTC ACCCTCAACT GCTCCCAATT GGTCAGAGCG GAGGGGTTCC AAATGTGCCG
    GTTGGCCAGA ATGGTGGGAT ATCTGGCATT TCCATTGGCC AAAGTGGTGG TGGGGCTCAG
    ACTTTCATTC AACCAGTCAC TATGGCTTCA CAGGTCCCAT TAATTGTGCC TCAACAATAT
    TCTCAGCCCC TTCCACCTTA CTCAGCAGAT GTTGCATCCT CACAAATGTT ACACTCCCGG
    CCTACTGATG CCTCCACTCC CCACAGTTCC CTAATACAGT CACAGACATA CATGCCCCCA
    GTATCGCCCC AGGCACCCAT GGCTGTCCTT ACGTCCTCTA TTCCGACTGG AGAACCAATA
    GCACCAGCAG GCAACATCAT GCCACCCATA CAGACCCAAA CTAGTATTCC TCCTCTTCAA
    CCCATGGATG TTTTGCCCCT GCAAACTGTT GTTCAAAATC AACAGCAAGT GATGCCTGAA
    TTGCCCAGAA CGCTGCAACC TAACATCCAG CAACCAACAC CACAACAGCA GGCAAATGTA
    ATGCAGCAGC ATCAGACAGT CTTGTTGCCA CAGCAAACTG ACCAGGTTCA GCAAATTAAA
    GTTTTGTTAC AACAGTCACA GCATGCAGAA CAACCCCAGG TGGTTCTGCA GGAGCAGGCC
    CCACATTCCA GTGCATCTCA ACAGCCATAT ATCCCATTAC CACAGCAAGC ACCGCCAACA
    ATGACCAACC CACAGCAAAG TGAGCTGCAA CAGCAGGTGT TTATACAGCA ACCTCAAAGC
    AAACCCCAAC AACAAGTTGG GATACAGCCT CAAGTTCAGC AGCATCAGAC ATCAATACAG
    CAACCCTCAG TTGATCAGTA TCAACACCAG ACTCAACAAC AGCAAGTGTA TGTGCCACAA
    ATAGCAGATC CACAAGACCA AAACGTGATA AAGACTGTGA TACAACAAGT ACCGGTGCTG
    CAAGCAATTC AGCAGCCATT ACAATTGATA GAGCAACAAC AGCAGCAGCT TCTGATTAGG
    CAACAAATTG AGCAACATCA GAAGGCTGCT TTACAAAACC AAATGGAGCA ACATCAACAG
    CAAGTTTATA TTCAGCCACC ACAACAGCAA AAAGTGGAAC AACAACAAAT TCTTATGCAG
    CAACAGCAAA ACTTGGCAGT AAAGCAACAG ATAGAGCAAC AGCAGCATGC ATTAAAGCAA
    CAAAAGCAAA TAGAGCAGCA GCAAAAAGCC ATTTTACAGC AACAGGCAGA ACAGCAAAGA
    CAGCAGCAAC AGACTTTGTT TCAGCAACAG CAGCTGGAGC AGCAACAAGT ATTACTGCAG
    CAGCAACAGC GTGAACAACA ACAGGCTCTT CTCCAGCAGC AACAGTTGGA GCAGCAAGCT
    TTACTGCAAC AACAGCAGCA ACTTCAGTTA AAGCAGCAAC AAGCTCTTCT ACATCAACAA
    CAGCTGGAGC AGCAGCAGCA GGCTTTGTTT AAACAGCAGC AGCTGGAGCA ACAGCAAGCT
    CTTTTGCAGC AGCAGCAGCA GCAAGCTCTC GTTCAGCAGC AGCAACAGTT AGAGCAAAAA
    GTACTGCTAC AGCTGCAACA GCAACAGCAG CAACACATCG ATCAGCAGCA AAATTTAATA
    CGCCAACAGC AGCCTCAGCA GATCGAGGCT GTTTTACAGC AACAACCACA ACTTCAGAAC
    TCAACTGATC AACAATTGCA GCAGCAAATA TTTCAACAAC AGACCGAGCA GCAACAGATG
    CAGCAGCATC TACTGATGAA ACAACAGCAG ATGGAATTAC AGCGACAACA AGCTCTACAA
    GCTGAGAAAC AACGACAGCA AGTTCTATTG CAACAACAAC ATTTGGAGCA ACATGCAGCT
    CTGGTGGATC ATAGTGGGCA GCAACAAGCA ACCCTGGTGA AAGCACAGTC TCCAGAAATG
    GTCCAGCAGA TTAGTCAAGT GCAGCAATCT CAACAAATGA TGTCTCTACA ACCGCAGCCT
    CATCTTGCTC AACAACAAAC TGACCAGACA CAGGTCTTGA CTCAGCAACA AGCCATTCTT
    CCCCAAGCAA CACACAGACC TTCTCTGGTA GACTTGCAAA TCCCAACCCT GCTTCAAGCA
    GCACCTAAAA TAGTCAGCAC TCAAATACCT ACCCAAGTTC AAGCCCCAGT ACTTTTGCAG
    CAACAGGTGC CACCTCAGAT GTCAGCTCAA CCTACAGCAC AAGTACAACT CCAGAGTCAA
    GGTGAACCAA CAATTTATAA CCAGATTGCA ATGCAGAGTC AAAGTCATCC CGCACAGCAG
    TCAATGGGAG GTCAAATTCA GACAGGCCAA TTGCAAATTC CACAATCAAC CCAACAAATC
    ACATCCCCAA CACAAGTTCC TACACCACAA CCCATTCATT CTTTTGTTCC AACTTCAACA
    CAGATGGTCT CACAAGGGCA AACCCTTCCA CCTCAACCTC AACTGGTACC CCCTCAAGCT
    GCAGCTTCTC TCCAGTGTCA AGTTGCAGTA GGGTCATCTG CACCTGCATC TTTTGTACAA
    GCTACAGTCC CAACTGCTTC TACACTTGTG CCCACACAGT ATGCTCAAGC ACCTGTTGCT
    TCTCAAACGA TCCAGTCTGT TCAGTTAGAC CTCACTCAAC CTGTGAAGCA GCAACAACAA
    CAACAGCCGC CTCTGCAGCA ATATCAGCAA GCGGTGCTAT CTCCGGTGTC ACTTGCACCT
    ACGATCAATC CAGTGGCTTC TTCCTCAACA CAGTGCTATT TACAGCAGAC AAACCAATCA
    CAAATGCAGG TCCAAACCCA ACAAGTAATT CAAGCTCAGC AACAACCAGT CAGTTTGGCC
    TCGATGCAGG CAGTCTTGCA GCCCCTGCCT TTAGTGCAGC CTCAGGCTAC CCAACAGTAT
    GAGCAGCATT TGCCTCAAGC CCACCATGTG CAACAACAAC CCCTCCACCA TCAGCAGCAG
    CTTCTGCCCC CAAATGCTGT TATACCACAA GATATCCACC AACAAACTTG TCAGCCTCAA
    CCAGTGCTCC AGCATGTGCT CCCATTACAC ACTGCACCCA GTCAGGTACC TCAGTCCCAA
    GCTAGTACAG TACCTCTCTA CAGTCAGGTG GTGTCAGCTT CCCCCCAGCA GAAATTAACT
    CTTCACACTC AAACACAGTC TAGCATGCAG TCACAAGTGC TCTCAAACTC ACAGGCACAG
    ACTCACATAG AGCCCACTGG AGCATTTGAG CATCCTCATT ACACCCAGGC TGCCTTTATG
    CCATCACAGA TTCCCACAAG TCCATCTCAT ACTTCCATAC AGCCCTCTTC CACTCTCCCA
    GCCCAACAAC CAGTTGTCCC AGTTTCAGAA ATTCCTGTCC CAGCAGACGC ACAGCTAACA
    CAGGCAAGGC CTGCTGATTT GATCCCTGCT TCCACTCCTA CTGTCAATAC TACCCAGTCC
    CATGACTCAA ACGGCTCTGT GCTGACAACC GCCATGCTGG CAGAAAGTGA CGCTGCATTG
    CTGGGCATTG CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGCC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACACTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TCTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC GCACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTAACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCAGATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GACGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATCGCAACA GCAACCAATG
    CAACAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATCCAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGAACAT GCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TGTGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGCTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGG CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACC GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCAAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGGGGTAAGA ACAGACGAGG GTAGAAAAGG GAGAGAATCT
    AAGAGGGATG ACCCTGGATC TGAGTCTCCA GTCTCACCGT ATGCAGTGCT GTTATTGCGG
    TGCCTGCACC CCTCACCATC CTGCACCCGC CTCACTCACC CACAGCACCT GAAATGGCTA
    AAATGGCACA CTTGTTTAAA AACTACATGG GCTGGACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27775
    Clone ID Related Accession (Same CDS sequence) XM_021469967.1 , XM_690703.8
    Accession Version XM_021469967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG ATGGCAACTC TAGCCAGGGG ATGAAGGAGG CATCTACAGA TGACCAACCC
    TGCTCACGCA GAGGCCGTTT CCAGGTGATT CCAGTATCCC AGTCTGCAGA GACCCTTCCA
    ACGCAGGAAG CTCCACCAAG CCAAATGATG AGAGTTGGCC GTTTCTCAGT CACTCAAGCT
    GAACATCGAG AGGAGCATTT GACCGACAGC TCCCCTGTGT CACCTGACCT GGAGCGAGAG
    AGGAGGAAAA CGAAAGACGA GAGGAGAACG ACAGCACCCA GCCACCCATC CAAGAGCCAC
    GCGCACTCAC CCCTGGGCAG CAGTGATGAT GAGAGCGAGG TGGAGGATGA AGACTTAAGG
    AAAGAACTGC ACAAACTCAG AGAGAAACAC ATCAAGGAGG TGGTGTCCCT GCAGGCACAG
    CAGAACAGAG AACTACAGGA TTTGTACAAG CAGCTCCGCT CTCTCAAAGA CCACAGACAG
    CCAATGTCTA TGAGCCTGTC CCGCACCTCT CCTCTGCCCT CCGCACCCCT CGCTATCTCC
    CCGCGCAGAC CCCGACCCGC CAAAGCCAAG CTCCGGCCTC GGCCGCACTC TCACATGGAT
    AACAACGGAG TCGCACACCA AGGGAGTCTT CAGCAGTCTA GCAGTTATTC CGGTGGGGAG
    CAAAGCCGGC TACATTCACA CTGCAAGCCA GAGAAATCAC AATCCCTGAC ATCTAACAGA
    GACCACAGTC CTTCAGCCCA GGGTTCTGGC AACAGAAAGA GCACATTCAC AGATGAGCTG
    CATAAACTTG TCGATGAGTG GTCAAAGGAA ACTGTTTGTC CAGTTCAGCC CAAGCCTTCG
    CTGAATCAGA TTAAACAGAT CCAACAAGTG CAGGAGTTGG GTGGATGGAG CCAGCCTGCT
    GAGACAACAG CTTCGTCGGG TTGGTTTTCC GGGGCCCCTT TAAGTACGCC TGTATCAGTC
    AGCATGACCA CCATGACATC CACCCCCTAT AGCGCCATTA GTGGACAAAC ACAGCCGCCT
    CCGACGCAAT TCCCTATCGT TCCTCTACCC CAGCAGAACG TGCATCCACA ACCCGGCCCG
    CACCGTCAGC CCCTGCAGTA CGGCCCTTCA CCCCTGCAGC AGGCCCAGCT CCCGGTGCCT
    GCAGCAGGAC AAACACCGCC CCCAACCAAT CCAGACGCTG TATTCTGCTC ATGCTCTTCC
    TCCTCCTGCT CCTCATCCTC CTGTTCCACC TCCAGTGCCA AACTTCACCC CAAACCCTCC
    ACGTCCACCC TTCCTTTAGG ACAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325642.1
    CDS1064..10093
    Translation

    Target ORF information:

    RefSeq Version XM_021469967.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG ATGGCAACTC TAGCCAGGGG ATGAAGGAGG CATCTACAGA TGACCAACCC
    TGCTCACGCA GAGGCCGTTT CCAGGTGATT CCAGTATCCC AGTCTGCAGA GACCCTTCCA
    ACGCAGGAAG CTCCACCAAG CCAAATGATG AGAGTTGGCC GTTTCTCAGT CACTCAAGCT
    GAACATCGAG AGGAGCATTT GACCGACAGC TCCCCTGTGT CACCTGACCT GGAGCGAGAG
    AGGAGGAAAA CGAAAGACGA GAGGAGAACG ACAGCACCCA GCCACCCATC CAAGAGCCAC
    GCGCACTCAC CCCTGGGCAG CAGTGATGAT GAGAGCGAGG TGGAGGATGA AGACTTAAGG
    AAAGAACTGC ACAAACTCAG AGAGAAACAC ATCAAGGAGG TGGTGTCCCT GCAGGCACAG
    CAGAACAGAG AACTACAGGA TTTGTACAAG CAGCTCCGCT CTCTCAAAGA CCACAGACAG
    CCAATGTCTA TGAGCCTGTC CCGCACCTCT CCTCTGCCCT CCGCACCCCT CGCTATCTCC
    CCGCGCAGAC CCCGACCCGC CAAAGCCAAG CTCCGGCCTC GGCCGCACTC TCACATGGAT
    AACAACGGAG TCGCACACCA AGGGAGTCTT CAGCAGTCTA GCAGTTATTC CGGTGGGGAG
    CAAAGCCGGC TACATTCACA CTGCAAGCCA GAGAAATCAC AATCCCTGAC ATCTAACAGA
    GACCACAGTC CTTCAGCCCA GGGTTCTGGC AACAGAAAGA GCACATTCAC AGATGAGCTG
    CATAAACTTG TCGATGAGTG GTCAAAGGAA ACTGTTTGTC CAGTTCAGCC CAAGCCTTCG
    CTGAATCAGA TTAAACAGAT CCAACAAGTG CAGGAGTTGG GTGGATGGAG CCAGCCTGCT
    GAGACAACAG CTTCGTCGGG TTGGTTTTCC GGGGCCCCTT TAAGTACGCC TGTATCAGTC
    AGCATGACCA CCATGACATC CACCCCCTAT AGCGCCATTA GTGGACAAAC ACAGCCGCCT
    CCGACGCAAT TCCCTATCGT TCCTCTACCC CAGCAGAACG TGCATCCACA ACCCGGCCCG
    CACCGTCAGC CCCTGCAGTA CGGCCCTTCA CCCCTGCAGC AGGCCCAGCT CCCGGTGCCT
    GCAGCAGGAC AAACACCGCC CCCAACCAAT CCAGACGCTG TATTCTGCTC ATGCTCTTCC
    TCCTCCTGCT CCTCATCCTC CTGTTCCACC TCCAGTGCCA AACTTCACCC CAAACCCTCC
    ACGTCCACCC TTCCTTTAGG ACAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27775
    Clone ID Related Accession (Same CDS sequence) XM_021469967.1 , XM_690703.8
    Accession Version XM_690703.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_690703.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG ATGGCAACTC TAGCCAGGGG ATGAAGGAGG CATCTACAGA TGACCAACCC
    TGCTCACGCA GAGGCCGTTT CCAGGTGATT CCAGTATCCC AGTCTGCAGA GACCCTTCCA
    ACGCAGGAAG CTCCACCAAG CCAAATGATG AGAGTTGGCC GTTTCTCAGT CACTCAAGCT
    GAACATCGAG AGGAGCATTT GACCGACAGC TCCCCTGTGT CACCTGACCT GGAGCGAGAG
    AGGAGGAAAA CGAAAGACGA GAGGAGAACG ACAGCACCCA GCCACCCATC CAAGAGCCAC
    GCGCACTCAC CCCTGGGCAG CAGTGATGAT GAGAGCGAGG TGGAGGATGA AGACTTAAGG
    AAAGAACTGC ACAAACTCAG AGAGAAACAC ATCAAGGAGG TGGTGTCCCT GCAGGCACAG
    CAGAACAGAG AACTACAGGA TTTGTACAAG CAGCTCCGCT CTCTCAAAGA CCACAGACAG
    CCAATGTCTA TGAGCCTGTC CCGCACCTCT CCTCTGCCCT CCGCACCCCT CGCTATCTCC
    CCGCGCAGAC CCCGACCCGC CAAAGCCAAG CTCCGGCCTC GGCCGCACTC TCACATGGAT
    AACAACGGAG TCGCACACCA AGGGAGTCTT CAGCAGTCTA GCAGTTATTC CGGTGGGGAG
    CAAAGCCGGC TACATTCACA CTGCAAGCCA GAGAAATCAC AATCCCTGAC ATCTAACAGA
    GACCACAGTC CTTCAGCCCA GGGTTCTGGC AACAGAAAGA GCACATTCAC AGATGAGCTG
    CATAAACTTG TCGATGAGTG GTCAAAGGAA ACTGTTTGTC CAGTTCAGCC CAAGCCTTCG
    CTGAATCAGA TTAAACAGAT CCAACAAGTG CAGGAGTTGG GTGGATGGAG CCAGCCTGCT
    GAGACAACAG CTTCGTCGGG TTGGTTTTCC GGGGCCCCTT TAAGTACGCC TGTATCAGTC
    AGCATGACCA CCATGACATC CACCCCCTAT AGCGCCATTA GTGGACAAAC ACAGCCGCCT
    CCGACGCAAT TCCCTATCGT TCCTCTACCC CAGCAGAACG TGCATCCACA ACCCGGCCCG
    CACCGTCAGC CCCTGCAGTA CGGCCCTTCA CCCCTGCAGC AGGCCCAGCT CCCGGTGCCT
    GCAGCAGGAC AAACACCGCC CCCAACCAAT CCAGACGCTG TATTCTGCTC ATGCTCTTCC
    TCCTCCTGCT CCTCATCCTC CTGTTCCACC TCCAGTGCCA AACTTCACCC CAAACCCTCC
    ACGTCCACCC TTCCTTTAGG ACAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_695795.6
    CDS996..10025
    Translation

    Target ORF information:

    RefSeq Version XM_690703.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio im:7152756 (im:7152756), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_690703.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG ATGGCAACTC TAGCCAGGGG ATGAAGGAGG CATCTACAGA TGACCAACCC
    TGCTCACGCA GAGGCCGTTT CCAGGTGATT CCAGTATCCC AGTCTGCAGA GACCCTTCCA
    ACGCAGGAAG CTCCACCAAG CCAAATGATG AGAGTTGGCC GTTTCTCAGT CACTCAAGCT
    GAACATCGAG AGGAGCATTT GACCGACAGC TCCCCTGTGT CACCTGACCT GGAGCGAGAG
    AGGAGGAAAA CGAAAGACGA GAGGAGAACG ACAGCACCCA GCCACCCATC CAAGAGCCAC
    GCGCACTCAC CCCTGGGCAG CAGTGATGAT GAGAGCGAGG TGGAGGATGA AGACTTAAGG
    AAAGAACTGC ACAAACTCAG AGAGAAACAC ATCAAGGAGG TGGTGTCCCT GCAGGCACAG
    CAGAACAGAG AACTACAGGA TTTGTACAAG CAGCTCCGCT CTCTCAAAGA CCACAGACAG
    CCAATGTCTA TGAGCCTGTC CCGCACCTCT CCTCTGCCCT CCGCACCCCT CGCTATCTCC
    CCGCGCAGAC CCCGACCCGC CAAAGCCAAG CTCCGGCCTC GGCCGCACTC TCACATGGAT
    AACAACGGAG TCGCACACCA AGGGAGTCTT CAGCAGTCTA GCAGTTATTC CGGTGGGGAG
    CAAAGCCGGC TACATTCACA CTGCAAGCCA GAGAAATCAC AATCCCTGAC ATCTAACAGA
    GACCACAGTC CTTCAGCCCA GGGTTCTGGC AACAGAAAGA GCACATTCAC AGATGAGCTG
    CATAAACTTG TCGATGAGTG GTCAAAGGAA ACTGTTTGTC CAGTTCAGCC CAAGCCTTCG
    CTGAATCAGA TTAAACAGAT CCAACAAGTG CAGGAGTTGG GTGGATGGAG CCAGCCTGCT
    GAGACAACAG CTTCGTCGGG TTGGTTTTCC GGGGCCCCTT TAAGTACGCC TGTATCAGTC
    AGCATGACCA CCATGACATC CACCCCCTAT AGCGCCATTA GTGGACAAAC ACAGCCGCCT
    CCGACGCAAT TCCCTATCGT TCCTCTACCC CAGCAGAACG TGCATCCACA ACCCGGCCCG
    CACCGTCAGC CCCTGCAGTA CGGCCCTTCA CCCCTGCAGC AGGCCCAGCT CCCGGTGCCT
    GCAGCAGGAC AAACACCGCC CCCAACCAAT CCAGACGCTG TATTCTGCTC ATGCTCTTCC
    TCCTCCTGCT CCTCATCCTC CTGTTCCACC TCCAGTGCCA AACTTCACCC CAAACCCTCC
    ACGTCCACCC TTCCTTTAGG ACAGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45152
    Clone ID Related Accession (Same CDS sequence) XM_021469966.1 , XM_009296926.2
    Accession Version XM_021469966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GATGGCAACT CTAGCCAGGG GATGAAGGAG
    GCATCTACAG ATGACCAACC CTGCTCACGC AGAGGCCGTT TCCAGGTGAT TCCAGTATCC
    CAGTCTGCAG AGACCCTTCC AACGCAGGAA GCTCCACCAA GCCAAATGAT GAGAGTTGGC
    CGTTTCTCAG TCACTCAAGC TGAACATCGA GAGGAGCATT TGACCGACAG CTCCCCTGTG
    TCACCTGACC TGGAGCGAGA GAGGAGGAAA ACGAAAGACG AGAGGAGAAC GACAGCACCC
    AGCCACCCAT CCAAGAGCCA CGCGCACTCA CCCCTGGGCA GCAGTGATGA TGAGAGCGAG
    GTGGAGGATG AAGACTTAAG GAAAGAACTG CACAAACTCA GAGAGAAACA CATCAAGGAG
    GTGGTGTCCC TGCAGGCACA GCAGAACAGA GAACTACAGG ATTTGTACAA GCAGCTCCGC
    TCTCTCAAAG ACCACAGACA GCCAATGTCT ATGAGCCTGT CCCGCACCTC TCCTCTGCCC
    TCCGCACCCC TCGCTATCTC CCCGCGCAGA CCCCGACCCG CCAAAGCCAA GCTCCGGCCT
    CGGCCGCACT CTCACATGGA TAACAACGGA GTCGCACACC AAGGGAGTCT TCAGCAGTCT
    AGCAGTTATT CCGGTGGGGA GCAAAGCCGG CTACATTCAC ACTGCAAGCC AGAGAAATCA
    CAATCCCTGA CATCTAACAG AGACCACAGT CCTTCAGCCC AGGGTTCTGG CAACAGAAAG
    AGCACATTCA CAGATGAGCT GCATAAACTT GTCGATGAGT GGTCAAAGGA AACTGTTTGT
    CCAGTTCAGC CCAAGCCTTC GCTGAATCAG ATTAAACAGA TCCAACAAGT GCAGGAGTTG
    GGTGGATGGA GCCAGCCTGC TGAGACAACA GCTTCGTCGG GTTGGTTTTC CGGGGCCCCT
    TTAAGTACGC CTGTATCAGT CAGCATGACC ACCATGACAT CCACCCCCTA TAGCGCCATT
    AGTGGACAAA CACAGCCGCC TCCGACGCAA TTCCCTATCG TTCCTCTACC CCAGCAGAAC
    GTGCATCCAC AACCCGGCCC GCACCGTCAG CCCCTGCAGT ACGGCCCTTC ACCCCTGCAG
    CAGGCCCAGC TCCCGGTGCC TGCAGCAGGA CAAACACCGC CCCCAACCAA TCCAGACGCT
    GTATTCTGCT CATGCTCTTC CTCCTCCTGC TCCTCATCCT CCTGTTCCAC CTCCAGTGCC
    AAACTTCACC CCAAACCCTC CACGTCCACC CTTCCTTTAG GACAGCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325641.1
    CDS1064..10234
    Translation

    Target ORF information:

    RefSeq Version XM_021469966.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GATGGCAACT CTAGCCAGGG GATGAAGGAG
    GCATCTACAG ATGACCAACC CTGCTCACGC AGAGGCCGTT TCCAGGTGAT TCCAGTATCC
    CAGTCTGCAG AGACCCTTCC AACGCAGGAA GCTCCACCAA GCCAAATGAT GAGAGTTGGC
    CGTTTCTCAG TCACTCAAGC TGAACATCGA GAGGAGCATT TGACCGACAG CTCCCCTGTG
    TCACCTGACC TGGAGCGAGA GAGGAGGAAA ACGAAAGACG AGAGGAGAAC GACAGCACCC
    AGCCACCCAT CCAAGAGCCA CGCGCACTCA CCCCTGGGCA GCAGTGATGA TGAGAGCGAG
    GTGGAGGATG AAGACTTAAG GAAAGAACTG CACAAACTCA GAGAGAAACA CATCAAGGAG
    GTGGTGTCCC TGCAGGCACA GCAGAACAGA GAACTACAGG ATTTGTACAA GCAGCTCCGC
    TCTCTCAAAG ACCACAGACA GCCAATGTCT ATGAGCCTGT CCCGCACCTC TCCTCTGCCC
    TCCGCACCCC TCGCTATCTC CCCGCGCAGA CCCCGACCCG CCAAAGCCAA GCTCCGGCCT
    CGGCCGCACT CTCACATGGA TAACAACGGA GTCGCACACC AAGGGAGTCT TCAGCAGTCT
    AGCAGTTATT CCGGTGGGGA GCAAAGCCGG CTACATTCAC ACTGCAAGCC AGAGAAATCA
    CAATCCCTGA CATCTAACAG AGACCACAGT CCTTCAGCCC AGGGTTCTGG CAACAGAAAG
    AGCACATTCA CAGATGAGCT GCATAAACTT GTCGATGAGT GGTCAAAGGA AACTGTTTGT
    CCAGTTCAGC CCAAGCCTTC GCTGAATCAG ATTAAACAGA TCCAACAAGT GCAGGAGTTG
    GGTGGATGGA GCCAGCCTGC TGAGACAACA GCTTCGTCGG GTTGGTTTTC CGGGGCCCCT
    TTAAGTACGC CTGTATCAGT CAGCATGACC ACCATGACAT CCACCCCCTA TAGCGCCATT
    AGTGGACAAA CACAGCCGCC TCCGACGCAA TTCCCTATCG TTCCTCTACC CCAGCAGAAC
    GTGCATCCAC AACCCGGCCC GCACCGTCAG CCCCTGCAGT ACGGCCCTTC ACCCCTGCAG
    CAGGCCCAGC TCCCGGTGCC TGCAGCAGGA CAAACACCGC CCCCAACCAA TCCAGACGCT
    GTATTCTGCT CATGCTCTTC CTCCTCCTGC TCCTCATCCT CCTGTTCCAC CTCCAGTGCC
    AAACTTCACC CCAAACCCTC CACGTCCACC CTTCCTTTAG GACAGCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45152
    Clone ID Related Accession (Same CDS sequence) XM_021469966.1 , XM_009296926.2
    Accession Version XM_009296926.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GATGGCAACT CTAGCCAGGG GATGAAGGAG
    GCATCTACAG ATGACCAACC CTGCTCACGC AGAGGCCGTT TCCAGGTGAT TCCAGTATCC
    CAGTCTGCAG AGACCCTTCC AACGCAGGAA GCTCCACCAA GCCAAATGAT GAGAGTTGGC
    CGTTTCTCAG TCACTCAAGC TGAACATCGA GAGGAGCATT TGACCGACAG CTCCCCTGTG
    TCACCTGACC TGGAGCGAGA GAGGAGGAAA ACGAAAGACG AGAGGAGAAC GACAGCACCC
    AGCCACCCAT CCAAGAGCCA CGCGCACTCA CCCCTGGGCA GCAGTGATGA TGAGAGCGAG
    GTGGAGGATG AAGACTTAAG GAAAGAACTG CACAAACTCA GAGAGAAACA CATCAAGGAG
    GTGGTGTCCC TGCAGGCACA GCAGAACAGA GAACTACAGG ATTTGTACAA GCAGCTCCGC
    TCTCTCAAAG ACCACAGACA GCCAATGTCT ATGAGCCTGT CCCGCACCTC TCCTCTGCCC
    TCCGCACCCC TCGCTATCTC CCCGCGCAGA CCCCGACCCG CCAAAGCCAA GCTCCGGCCT
    CGGCCGCACT CTCACATGGA TAACAACGGA GTCGCACACC AAGGGAGTCT TCAGCAGTCT
    AGCAGTTATT CCGGTGGGGA GCAAAGCCGG CTACATTCAC ACTGCAAGCC AGAGAAATCA
    CAATCCCTGA CATCTAACAG AGACCACAGT CCTTCAGCCC AGGGTTCTGG CAACAGAAAG
    AGCACATTCA CAGATGAGCT GCATAAACTT GTCGATGAGT GGTCAAAGGA AACTGTTTGT
    CCAGTTCAGC CCAAGCCTTC GCTGAATCAG ATTAAACAGA TCCAACAAGT GCAGGAGTTG
    GGTGGATGGA GCCAGCCTGC TGAGACAACA GCTTCGTCGG GTTGGTTTTC CGGGGCCCCT
    TTAAGTACGC CTGTATCAGT CAGCATGACC ACCATGACAT CCACCCCCTA TAGCGCCATT
    AGTGGACAAA CACAGCCGCC TCCGACGCAA TTCCCTATCG TTCCTCTACC CCAGCAGAAC
    GTGCATCCAC AACCCGGCCC GCACCGTCAG CCCCTGCAGT ACGGCCCTTC ACCCCTGCAG
    CAGGCCCAGC TCCCGGTGCC TGCAGCAGGA CAAACACCGC CCCCAACCAA TCCAGACGCT
    GTATTCTGCT CATGCTCTTC CTCCTCCTGC TCCTCATCCT CCTGTTCCAC CTCCAGTGCC
    AAACTTCACC CCAAACCCTC CACGTCCACC CTTCCTTTAG GACAGCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295201.1
    CDS996..10166
    Translation

    Target ORF information:

    RefSeq Version XM_009296926.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio im:7152756 (im:7152756), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296926.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GATGGCAACT CTAGCCAGGG GATGAAGGAG
    GCATCTACAG ATGACCAACC CTGCTCACGC AGAGGCCGTT TCCAGGTGAT TCCAGTATCC
    CAGTCTGCAG AGACCCTTCC AACGCAGGAA GCTCCACCAA GCCAAATGAT GAGAGTTGGC
    CGTTTCTCAG TCACTCAAGC TGAACATCGA GAGGAGCATT TGACCGACAG CTCCCCTGTG
    TCACCTGACC TGGAGCGAGA GAGGAGGAAA ACGAAAGACG AGAGGAGAAC GACAGCACCC
    AGCCACCCAT CCAAGAGCCA CGCGCACTCA CCCCTGGGCA GCAGTGATGA TGAGAGCGAG
    GTGGAGGATG AAGACTTAAG GAAAGAACTG CACAAACTCA GAGAGAAACA CATCAAGGAG
    GTGGTGTCCC TGCAGGCACA GCAGAACAGA GAACTACAGG ATTTGTACAA GCAGCTCCGC
    TCTCTCAAAG ACCACAGACA GCCAATGTCT ATGAGCCTGT CCCGCACCTC TCCTCTGCCC
    TCCGCACCCC TCGCTATCTC CCCGCGCAGA CCCCGACCCG CCAAAGCCAA GCTCCGGCCT
    CGGCCGCACT CTCACATGGA TAACAACGGA GTCGCACACC AAGGGAGTCT TCAGCAGTCT
    AGCAGTTATT CCGGTGGGGA GCAAAGCCGG CTACATTCAC ACTGCAAGCC AGAGAAATCA
    CAATCCCTGA CATCTAACAG AGACCACAGT CCTTCAGCCC AGGGTTCTGG CAACAGAAAG
    AGCACATTCA CAGATGAGCT GCATAAACTT GTCGATGAGT GGTCAAAGGA AACTGTTTGT
    CCAGTTCAGC CCAAGCCTTC GCTGAATCAG ATTAAACAGA TCCAACAAGT GCAGGAGTTG
    GGTGGATGGA GCCAGCCTGC TGAGACAACA GCTTCGTCGG GTTGGTTTTC CGGGGCCCCT
    TTAAGTACGC CTGTATCAGT CAGCATGACC ACCATGACAT CCACCCCCTA TAGCGCCATT
    AGTGGACAAA CACAGCCGCC TCCGACGCAA TTCCCTATCG TTCCTCTACC CCAGCAGAAC
    GTGCATCCAC AACCCGGCCC GCACCGTCAG CCCCTGCAGT ACGGCCCTTC ACCCCTGCAG
    CAGGCCCAGC TCCCGGTGCC TGCAGCAGGA CAAACACCGC CCCCAACCAA TCCAGACGCT
    GTATTCTGCT CATGCTCTTC CTCCTCCTGC TCCTCATCCT CCTGTTCCAC CTCCAGTGCC
    AAACTTCACC CCAAACCCTC CACGTCCACC CTTCCTTTAG GACAGCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45153
    Clone ID Related Accession (Same CDS sequence) XM_009296927.2 , XM_021469968.1
    Accession Version XM_009296927.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    301
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    601
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    721
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    4141
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    4261
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    4441
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    4681
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    6001
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    6301
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    6421
    6481
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    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTC CCCTTCCACC TTACTCAGCA GATGTTGCAT CCTCACAAAT GTTACACTCC
    CGGCCTACTG ATGCCTCCAC TCCCCACAGT TCCCTAATAC AGTCACAGAC ATACATGCCC
    CCAGTATCGC CCCAGGCACC CATGGCTGTC CTTACGTCCT CTATTCCGAC TGGAGAACCA
    ATAGCACCAG CAGGCAACAT CATGCCACCC ATACAGACCC AAACTAGTAT TCCTCCTCTT
    CAACCCATGG ATGTTTTGCC CCTGCAAACT GTTGTTCAAA ATCAACAGCA AGTGATGCCT
    GAATTGCCCA GAACGCTGCA ACCTAACATC CAGCAACCAA CACCACAACA GCAGGCAAAT
    GTAATGCAGC AGCATCAGAC AGTCTTGTTG CCACAGCAAA CTGACCAGGT TCAGCAAATT
    AAAGTTTTGT TACAACAGTC ACAGCATGCA GAACAACCCC AGGTGGTTCT GCAGGAGCAG
    GCCCCACATT CCAGTGCATC TCAACAGCCA TATATCCCAT TACCACAGCA AGCACCGCCA
    ACAATGACCA ACCCACAGCA AAGTGAGCTG CAACAGCAGG TGTTTATACA GCAACCTCAA
    AGCAAACCCC AACAACAAGT TGGGATACAG CCTCAAGTTC AGCAGCATCA GACATCAATA
    CAGCAACCCT CAGTTGATCA GTATCAACAC CAGACTCAGC AACAGCAAGT GTATGTGCCA
    CAAATGGCAG AATCACAAGA CCAGAACGTG ATAAAGACTG TGATACAACA GGTACCGGTG
    CTGCAAGCAA TTCAGCAGCC ATTACAATTG ATAGAGCAAC AACAGCAGCA GCTTCTGATT
    AGGCAACAAA TTGAGCAACA TCAGAAGGCT GCTTTACAAA ACCAAATGGA GCAACATCAA
    CAGCAAGTTT ATATTCAGCC ACCACAACAG CAAAAAGTGG AACAACAACA AATTCTTATG
    CAGCAACAGC AAAACTTGGC AGTAAAGCAA CAGATAGAGC AACAGCAGCA TGCATTAAAG
    CAACAAAAGC AAATAGAGCA GCAGCAAAAA GCCATTTTAC AGCAACAGGC AGAACAGCAA
    AGACAGCAGC AACAGACTTT GTTTCAGCAA CAGCAGCTGG AGCAGCAACA AGTATTACTG
    CAGCAGCAAC AGCGTGAACA ACAACAGGCT CTTCTCCAGC AGCAACAGTT GGAGCAGCAA
    GCTTTACTGC AACAACAGCA GCAACTTCAG TTAAAGCAGC AACAAGCTCT TCTACATCAA
    CAACAGCTGG AGCAGCAGCA GCAGGCTTTG TTTAAACAGC AGCAGCTGGA GCAACAGCAA
    GCTCTTTTGC AGCAGCAGCA GCAGCAAGCT CTCGTTCAGC AGCAGCAACA GTTAGAGCAA
    AAAGTACTGC TACAGCTGCA ACAGCAACAG CAGCAACACA TCGATCAGCA GCAAAATTTA
    ATACGCCAAC AGCAGCCTCA GCAGATCGAG GCTGTTTTAC AGCAACAACC ACAACTTCAG
    AACTCAACTG ATCAACAATT GCAGCAGCAA ATATTTCAAC AACAGACCGA GCAGCAACAG
    ATGCAGCAGC ATCTACTGAT GAAACAACAG CAGATGGAAT TACAGCGACA ACAAGCTCTA
    CAAGCTGAGA AACAACGACA GCAAGTTCTA TTGCAACAAC AACATTTGGA GCAACATGCA
    GCTCTGGTGG ATCATAGTGG GCAGCAACAA GCAACCCTGG TGAAAGCACA GTCTCCAGAA
    ATGGTCCAGC AGATTAGTCA AGTGCAGCAA TCTCAACAAA TGATGTCTCT ACAACCGCAG
    CCTCATCTTG CTCAACAACA AACTGACCAG ACCCAGGTCT TGACTCAGCA ACAAGCCATT
    CTTCCCCAAG CAACACACAG ACCTTCTCTG GTAGACTTGC AAATCCCAAC CCTGCTTCAA
    GCAGCACCTA AAATAGTCAG CACTCAAATA CCTACCCAAG TTCAAGCCCC AGTACTTTTG
    CAGCAACAGG TGCCACCTCA GATGTCAGCT CAACCTACAG CACAAGTACA ACTCCAGAGT
    CAAGGTGAAC CAACAATTTA TGACCAGATT GCAATGCAGA GTCAAAGTCA TCCCGCACAG
    CAGTCAATGG GAGGTCAAAT TCAGACAGGC CAATTGCAAA TTCCACAATC AACCCAACAA
    ATCACATCCC CAACACAAGT TCCTACACCA CAACCCATTC ATTCTTTTGT TCCAACTTCA
    ACACAGATGG TCTCACAAGG GCAAACCCTT CCACCTCAAC CTCAACTGGT ACCCCCTCAA
    GCTGCAGCTT CTCTCCAGTG TCAAGTTGCA GTAGGGTCAT CTGCACCTGC ATCTTTTGTT
    CAAGCTACAG TCCCAACTGC TTCTACACTT GTGCCCACAC AGTATGCTCA AGCACCTGTT
    GCTTCTCAAA CGATCCAGTC TGTTCAGTTA GACCTCACTC AACCTGTGAA GCAGCAACAA
    CAACAACAGC CGCCTCTGCA GCAATATCAG CAAGCGGTGC TATCTCCGGT GTCACTTGCA
    CCTACGATCA ATCCAGTGGC TTCTTCCTCA ACACAGTGCT ATTTACAGCA GACAAACCAA
    TCACAAATTC AGGTCCAAAC CCAACAAGTA ATTCAAGCTC AGCAACAACC AGTCAGTTTG
    GCCTCGATGC AGGCATTCTT GCAGCCCCTA CCTTTAGTGC AGCCTCAGGC TACCCAACAG
    TATGAGCAGC ATTTGCCTCA AGCCCACCAC GTGCAACAAC AACCCCTCCA CCATCAGCAG
    CAGCTTCTGC CCCCAAATGC TGTTATACCA CAAGATATCC ACCAACAAAC GTGTCAGCCT
    CAACCAGTGC TCCAGCATGT GCTCCCATTA CACACTGCAC CCAGTCAGGT ACCTCAGTCC
    CAAGCTAGTA CAGTACCTCT CTACAGTCAG GTGGTGTCAG CTTCCCCCCA GCAGAAATTA
    ACTCTTCACA CTCAAACACA GTCTAGCATG CAGTCACAAG TGCTCTCAAA CTCACAGGCA
    CAGACTCACA TAGAGCCCAC TGGAGCATTT GAGCATCCTC ATTACACCCA GGCTGCCTTT
    ATGCCATCAC AGATTCCCAC AAGTCCATCT CATACTTCCA TACAGCCCTC TTCCACTCTC
    CCAGCCCAAC AACCAGTTGT CCCAGTTTCA GAAATTCCTG TCCCAGCAGA CGCACAGCTA
    ACACAGGCAA GGCCTGCTGA TTTGATCCCT GCTTCCACTC CTACTGTCAA TACTACCCAG
    TCCCATGACT CAAACGGCTC TGTGCTGACA ACCGCCATGC TGGCAGAAAG TGACGCTGCA
    TTGCTGGGCA TTGCTCAGGA AAGCCTAATT CACTCTGCCA ACAGGGGATC TTTATTATGC
    TCTGCTCCTA CCAATGGAGA TGAGGGCCAG TTGGTGTGGC CTAGTGGAAA ACTAGATAAG
    GCAAAATGTC AGAGGAGATC ATCCTGTCAG AAGACTGAAA AAACTCACTT CCAACTCTGC
    ATGTTGCAGG TCTCATGCAC AGGAGACAAC ATGGTAGAAT GTCAGTTGGA GACTCACAGC
    AACAAGATGG TCACGTTCAA GTTTGACATT GAGGGAGATG CACCTGAAGA CATTGCAGAT
    TACATGGTGG AGGAGGACTT CGTCCTTGAG CCTGAAAAAG AAAAATTTGT TGAGGAGCTG
    AGATGTATAG TGAAGAAAGC TCAAGCAATA TTAAGTACTC AGTCGCAGAC TGGATCCCTG
    GAGCAGCTCC AAGTGAGCAC CCCATCCAGT GCAACAATGG ATTCAGCCCC TCAGTCTTCT
    CCAGTAGGTC GCTGGCGGTT CTTCATTAAC CAGACTATTC GTCACCGTGA TTCACACTCC
    AACCAGGGAG CTTCGACTCC CCCTCCAGTG GGGGAGAACA GAGTTCCCAA GTCCATAGAA
    ACCGAGAAAG ATCATGAGAG CCCGCAGCGC TCCGAGTCTT TTGCTGGAAT AGCATCTTCC
    CCAAGTTCTT CACTATCTGC ATCCTCTCCT CCAGTCTCTA CTGTATCCGC TCCTGCATCC
    ATGTCAGCCT CAGCCATGGC TGCTCAAAAA GATTTTACAC CTGCTGCCTT GCCAGCAGTA
    GAGAAAGTCT CCTCTACTAT GCTGACCGCA GGTCCAGTCC CTGTCATGGC CTCCAGCACT
    TCTGCCACTC CTTTTCCTGC AGCCATGGCC CTCACAGGCT CTAATGTGGA CCAAGGAGAA
    TCTGCTGTTA AAGGTGAGGG TCAACAGCCT TATGTGACTC CTCCAACGTC ACTGTTGACC
    CATGCTGACC CATCTCAGCA CTTAATTGTT CAAACCCCAA TGGCACCCTC ACAGATGCCA
    GATATACCTC AACAAAGCCA GACACTTCAA TCAACATTGA TCACAACTGA ACAAGTTCAG
    CAACCGCAGA TTACCCCTCA GCCACTTCTG CAGGATGCTC CACAGCTACC TAAACCAGAA
    TCAAAATCGC TACCACAGGC AGTGCCTAAA GAGATGGTTC CTTTGCATGA AGGTCTTTCA
    GCTGCCCAAG TTCAGCAGGT CCCTCTCCAG CAAGTACCAG TAGAATTGCA ACAGCAGCCA
    ATGCAGCAGA CCACATTCTT ACAGCAAACA TCCAGTGCAG AGCAGATACA GCAGCACATG
    GCAGAACAGT TTTTATCTCA AGAAACCAAA TCAGCTCCAA TGATACTGAC CATTCATCCA
    CAGCATGGCT TCCAGACCCA GGATCCTCAG CAGGTTCAAG CTCCATTGCA GTCTATGATC
    CCCCAGCAGG CTCCAATTGA GCAAACCCAG ACATTATATA AACAGGTCCC ACATGATATG
    ATGCCAGTGG CAAAAACCGC AGTGCAGAAG CAAGTGTCTT TCCAGCAGTC AGAGTCTGAG
    CTGTCTACGG GTGAGGACAC CGTCAGCCAC TCTGCACTTC CGCATCCCTC TTTAGACACC
    TCTCTTCCAC CCCTCCCTCT CTCTGAAACA GCTCTTCCTG CCCTCTCCCA CACATTCACT
    CCTTCTCCAG CACAGCCTTC TTCAGTAGCA GAGTCGGACA GTGAAGGCCC TCCCAAAATT
    GAGTTTGTGG ATAACCGAAT CAAGACTTTG GATGAGAAAT TGAGGACTCT GCTCTATCAG
    GAGCACGGCG GCAGTGGAGC AGCTGTGGGC AGTGCCTCCA CAGGAGGAAC TGCTCCAGCC
    TGCTCCACTT CAGGATCTGC CACAGCCTCT ACTGCAGTTG ATGAGTCTTC AGAAGTCCCC
    TACGCATTCT CCCTACCTCC TGCCACCTCC TCGGACACCT CCCCTCACTC CACCTCTTCC
    ACAACCTCCT CCACCACCCC TCGCTCTTCC TCCACCTCCC CGGATGGAGA GCGAGAGAAA
    GCAGGCGAGG AGACGACGAC TATCCCACCT GCGGTACAAC AACAGCCTGC CTCCTCTACA
    TCCCCTCCAT GCTCTCTTCT TTCCCATCCA CAGGAGCCTG GATCCCCCAA AGTTACTATT
    CAATCATCAG TAAGCGCTCC CCCTTCCCTC TCTGACAGCT GCACCCCTGG AGATGTTCTG
    TTGCCTTCCT CTTCTCAGCC GCCCGTCCCA CTGTGGCCTG GACAGCAGCA GCACAATGAA
    GGAGGTGGAT ATTTTGGCCT AAACCTGACA TGTCCTAGTA TCAGAAATCC TGTTAGCAAG
    AAATCCTGGA CTCGCAAATT CAAAAGCTGG GCATGCAAAC TGCGCCACTC CGCCAGCTTG
    TTCAAGAAGC CCAGAGTCCA GCAAGATGGC AACTCTAGCC AGGGGATGAA GGAGGCATCT
    ACAGATGACC AACCCTGCTC ACGCAGAGGC CGTTTCCAGG TGATTCCAGT ATCCCAGTCT
    GCAGAGACCC TTCCAACGCA GGAAGCTCCA CCAAGCCAAA TGATGAGAGT TGGCCGTTTC
    TCAGTCACTC AAGCTGAACA TCGAGAGGAG CATTTGACCG ACAGCTCCCC TGTGTCACCT
    GACCTGGAGC GAGAGAGGAG GAAAACGAAA GACGAGAGGA GAACGACAGC ACCCAGCCAC
    CCATCCAAGA GCCACGCGCA CTCACCCCTG GGCAGCAGTG ATGATGAGAG CGAGGTGGAG
    GATGAAGACT TAAGGAAAGA ACTGCACAAA CTCAGAGAGA AACACATCAA GGAGGTGGTG
    TCCCTGCAGG CACAGCAGAA CAGAGAACTA CAGGATTTGT ACAAGCAGCT CCGCTCTCTC
    AAAGACCACA GACAGCCAAT GTCTATGAGC CTGTCCCGCA CCTCTCCTCT GCCCTCCGCA
    CCCCTCGCTA TCTCCCCGCG CAGACCCCGA CCCGCCAAAG CCAAGCTCCG GCCTCGGCCG
    CACTCTCACA TGGATAACAA CGGAGTCGCA CACCAAGGGA GTCTTCAGCA GTCTAGCAGT
    TATTCCGGTG GGGAGCAAAG CCGGCTACAT TCACACTGCA AGCCAGAGAA ATCACAATCC
    CTGACATCTA ACAGAGACCA CAGTCCTTCA GCCCAGGGTT CTGGCAACAG AAAGAGCACA
    TTCACAGATG AGCTGCATAA ACTTGTCGAT GAGTGGTCAA AGGAAACTGT TTGTCCAGTT
    CAGCCCAAGC CTTCGCTGAA TCAGATTAAA CAGATCCAAC AAGTGCAGGA GTTGGGTGGA
    TGGAGCCAGC CTGCTGAGAC AACAGCTTCG TCGGGTTGGT TTTCCGGGGC CCCTTTAAGT
    ACGCCTGTAT CAGTCAGCAT GACCACCATG ACATCCACCC CCTATAGCGC CATTAGTGGA
    CAAACACAGC CGCCTCCGAC GCAATTCCCT ATCGTTCCTC TACCCCAGCA GAACGTGCAT
    CCACAACCCG GCCCGCACCG TCAGCCCCTG CAGTACGGCC CTTCACCCCT GCAGCAGGCC
    CAGCTCCCGG TGCCTGCAGC AGGACAAACA CCGCCCCCAA CCAATCCAGA CGCTGTATTC
    TGCTCATGCT CTTCCTCCTC CTGCTCCTCA TCCTCCTGTT CCACCTCCAG TGCCAAACTT
    CACCCCAAAC CCTCCACGTC CACCCTTCCT TTAGGACAGC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295202.1
    CDS996..9980
    Translation

    Target ORF information:

    RefSeq Version XM_009296927.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio im:7152756 (im:7152756), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296927.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTC CCCTTCCACC TTACTCAGCA GATGTTGCAT CCTCACAAAT GTTACACTCC
    CGGCCTACTG ATGCCTCCAC TCCCCACAGT TCCCTAATAC AGTCACAGAC ATACATGCCC
    CCAGTATCGC CCCAGGCACC CATGGCTGTC CTTACGTCCT CTATTCCGAC TGGAGAACCA
    ATAGCACCAG CAGGCAACAT CATGCCACCC ATACAGACCC AAACTAGTAT TCCTCCTCTT
    CAACCCATGG ATGTTTTGCC CCTGCAAACT GTTGTTCAAA ATCAACAGCA AGTGATGCCT
    GAATTGCCCA GAACGCTGCA ACCTAACATC CAGCAACCAA CACCACAACA GCAGGCAAAT
    GTAATGCAGC AGCATCAGAC AGTCTTGTTG CCACAGCAAA CTGACCAGGT TCAGCAAATT
    AAAGTTTTGT TACAACAGTC ACAGCATGCA GAACAACCCC AGGTGGTTCT GCAGGAGCAG
    GCCCCACATT CCAGTGCATC TCAACAGCCA TATATCCCAT TACCACAGCA AGCACCGCCA
    ACAATGACCA ACCCACAGCA AAGTGAGCTG CAACAGCAGG TGTTTATACA GCAACCTCAA
    AGCAAACCCC AACAACAAGT TGGGATACAG CCTCAAGTTC AGCAGCATCA GACATCAATA
    CAGCAACCCT CAGTTGATCA GTATCAACAC CAGACTCAGC AACAGCAAGT GTATGTGCCA
    CAAATGGCAG AATCACAAGA CCAGAACGTG ATAAAGACTG TGATACAACA GGTACCGGTG
    CTGCAAGCAA TTCAGCAGCC ATTACAATTG ATAGAGCAAC AACAGCAGCA GCTTCTGATT
    AGGCAACAAA TTGAGCAACA TCAGAAGGCT GCTTTACAAA ACCAAATGGA GCAACATCAA
    CAGCAAGTTT ATATTCAGCC ACCACAACAG CAAAAAGTGG AACAACAACA AATTCTTATG
    CAGCAACAGC AAAACTTGGC AGTAAAGCAA CAGATAGAGC AACAGCAGCA TGCATTAAAG
    CAACAAAAGC AAATAGAGCA GCAGCAAAAA GCCATTTTAC AGCAACAGGC AGAACAGCAA
    AGACAGCAGC AACAGACTTT GTTTCAGCAA CAGCAGCTGG AGCAGCAACA AGTATTACTG
    CAGCAGCAAC AGCGTGAACA ACAACAGGCT CTTCTCCAGC AGCAACAGTT GGAGCAGCAA
    GCTTTACTGC AACAACAGCA GCAACTTCAG TTAAAGCAGC AACAAGCTCT TCTACATCAA
    CAACAGCTGG AGCAGCAGCA GCAGGCTTTG TTTAAACAGC AGCAGCTGGA GCAACAGCAA
    GCTCTTTTGC AGCAGCAGCA GCAGCAAGCT CTCGTTCAGC AGCAGCAACA GTTAGAGCAA
    AAAGTACTGC TACAGCTGCA ACAGCAACAG CAGCAACACA TCGATCAGCA GCAAAATTTA
    ATACGCCAAC AGCAGCCTCA GCAGATCGAG GCTGTTTTAC AGCAACAACC ACAACTTCAG
    AACTCAACTG ATCAACAATT GCAGCAGCAA ATATTTCAAC AACAGACCGA GCAGCAACAG
    ATGCAGCAGC ATCTACTGAT GAAACAACAG CAGATGGAAT TACAGCGACA ACAAGCTCTA
    CAAGCTGAGA AACAACGACA GCAAGTTCTA TTGCAACAAC AACATTTGGA GCAACATGCA
    GCTCTGGTGG ATCATAGTGG GCAGCAACAA GCAACCCTGG TGAAAGCACA GTCTCCAGAA
    ATGGTCCAGC AGATTAGTCA AGTGCAGCAA TCTCAACAAA TGATGTCTCT ACAACCGCAG
    CCTCATCTTG CTCAACAACA AACTGACCAG ACCCAGGTCT TGACTCAGCA ACAAGCCATT
    CTTCCCCAAG CAACACACAG ACCTTCTCTG GTAGACTTGC AAATCCCAAC CCTGCTTCAA
    GCAGCACCTA AAATAGTCAG CACTCAAATA CCTACCCAAG TTCAAGCCCC AGTACTTTTG
    CAGCAACAGG TGCCACCTCA GATGTCAGCT CAACCTACAG CACAAGTACA ACTCCAGAGT
    CAAGGTGAAC CAACAATTTA TGACCAGATT GCAATGCAGA GTCAAAGTCA TCCCGCACAG
    CAGTCAATGG GAGGTCAAAT TCAGACAGGC CAATTGCAAA TTCCACAATC AACCCAACAA
    ATCACATCCC CAACACAAGT TCCTACACCA CAACCCATTC ATTCTTTTGT TCCAACTTCA
    ACACAGATGG TCTCACAAGG GCAAACCCTT CCACCTCAAC CTCAACTGGT ACCCCCTCAA
    GCTGCAGCTT CTCTCCAGTG TCAAGTTGCA GTAGGGTCAT CTGCACCTGC ATCTTTTGTT
    CAAGCTACAG TCCCAACTGC TTCTACACTT GTGCCCACAC AGTATGCTCA AGCACCTGTT
    GCTTCTCAAA CGATCCAGTC TGTTCAGTTA GACCTCACTC AACCTGTGAA GCAGCAACAA
    CAACAACAGC CGCCTCTGCA GCAATATCAG CAAGCGGTGC TATCTCCGGT GTCACTTGCA
    CCTACGATCA ATCCAGTGGC TTCTTCCTCA ACACAGTGCT ATTTACAGCA GACAAACCAA
    TCACAAATTC AGGTCCAAAC CCAACAAGTA ATTCAAGCTC AGCAACAACC AGTCAGTTTG
    GCCTCGATGC AGGCATTCTT GCAGCCCCTA CCTTTAGTGC AGCCTCAGGC TACCCAACAG
    TATGAGCAGC ATTTGCCTCA AGCCCACCAC GTGCAACAAC AACCCCTCCA CCATCAGCAG
    CAGCTTCTGC CCCCAAATGC TGTTATACCA CAAGATATCC ACCAACAAAC GTGTCAGCCT
    CAACCAGTGC TCCAGCATGT GCTCCCATTA CACACTGCAC CCAGTCAGGT ACCTCAGTCC
    CAAGCTAGTA CAGTACCTCT CTACAGTCAG GTGGTGTCAG CTTCCCCCCA GCAGAAATTA
    ACTCTTCACA CTCAAACACA GTCTAGCATG CAGTCACAAG TGCTCTCAAA CTCACAGGCA
    CAGACTCACA TAGAGCCCAC TGGAGCATTT GAGCATCCTC ATTACACCCA GGCTGCCTTT
    ATGCCATCAC AGATTCCCAC AAGTCCATCT CATACTTCCA TACAGCCCTC TTCCACTCTC
    CCAGCCCAAC AACCAGTTGT CCCAGTTTCA GAAATTCCTG TCCCAGCAGA CGCACAGCTA
    ACACAGGCAA GGCCTGCTGA TTTGATCCCT GCTTCCACTC CTACTGTCAA TACTACCCAG
    TCCCATGACT CAAACGGCTC TGTGCTGACA ACCGCCATGC TGGCAGAAAG TGACGCTGCA
    TTGCTGGGCA TTGCTCAGGA AAGCCTAATT CACTCTGCCA ACAGGGGATC TTTATTATGC
    TCTGCTCCTA CCAATGGAGA TGAGGGCCAG TTGGTGTGGC CTAGTGGAAA ACTAGATAAG
    GCAAAATGTC AGAGGAGATC ATCCTGTCAG AAGACTGAAA AAACTCACTT CCAACTCTGC
    ATGTTGCAGG TCTCATGCAC AGGAGACAAC ATGGTAGAAT GTCAGTTGGA GACTCACAGC
    AACAAGATGG TCACGTTCAA GTTTGACATT GAGGGAGATG CACCTGAAGA CATTGCAGAT
    TACATGGTGG AGGAGGACTT CGTCCTTGAG CCTGAAAAAG AAAAATTTGT TGAGGAGCTG
    AGATGTATAG TGAAGAAAGC TCAAGCAATA TTAAGTACTC AGTCGCAGAC TGGATCCCTG
    GAGCAGCTCC AAGTGAGCAC CCCATCCAGT GCAACAATGG ATTCAGCCCC TCAGTCTTCT
    CCAGTAGGTC GCTGGCGGTT CTTCATTAAC CAGACTATTC GTCACCGTGA TTCACACTCC
    AACCAGGGAG CTTCGACTCC CCCTCCAGTG GGGGAGAACA GAGTTCCCAA GTCCATAGAA
    ACCGAGAAAG ATCATGAGAG CCCGCAGCGC TCCGAGTCTT TTGCTGGAAT AGCATCTTCC
    CCAAGTTCTT CACTATCTGC ATCCTCTCCT CCAGTCTCTA CTGTATCCGC TCCTGCATCC
    ATGTCAGCCT CAGCCATGGC TGCTCAAAAA GATTTTACAC CTGCTGCCTT GCCAGCAGTA
    GAGAAAGTCT CCTCTACTAT GCTGACCGCA GGTCCAGTCC CTGTCATGGC CTCCAGCACT
    TCTGCCACTC CTTTTCCTGC AGCCATGGCC CTCACAGGCT CTAATGTGGA CCAAGGAGAA
    TCTGCTGTTA AAGGTGAGGG TCAACAGCCT TATGTGACTC CTCCAACGTC ACTGTTGACC
    CATGCTGACC CATCTCAGCA CTTAATTGTT CAAACCCCAA TGGCACCCTC ACAGATGCCA
    GATATACCTC AACAAAGCCA GACACTTCAA TCAACATTGA TCACAACTGA ACAAGTTCAG
    CAACCGCAGA TTACCCCTCA GCCACTTCTG CAGGATGCTC CACAGCTACC TAAACCAGAA
    TCAAAATCGC TACCACAGGC AGTGCCTAAA GAGATGGTTC CTTTGCATGA AGGTCTTTCA
    GCTGCCCAAG TTCAGCAGGT CCCTCTCCAG CAAGTACCAG TAGAATTGCA ACAGCAGCCA
    ATGCAGCAGA CCACATTCTT ACAGCAAACA TCCAGTGCAG AGCAGATACA GCAGCACATG
    GCAGAACAGT TTTTATCTCA AGAAACCAAA TCAGCTCCAA TGATACTGAC CATTCATCCA
    CAGCATGGCT TCCAGACCCA GGATCCTCAG CAGGTTCAAG CTCCATTGCA GTCTATGATC
    CCCCAGCAGG CTCCAATTGA GCAAACCCAG ACATTATATA AACAGGTCCC ACATGATATG
    ATGCCAGTGG CAAAAACCGC AGTGCAGAAG CAAGTGTCTT TCCAGCAGTC AGAGTCTGAG
    CTGTCTACGG GTGAGGACAC CGTCAGCCAC TCTGCACTTC CGCATCCCTC TTTAGACACC
    TCTCTTCCAC CCCTCCCTCT CTCTGAAACA GCTCTTCCTG CCCTCTCCCA CACATTCACT
    CCTTCTCCAG CACAGCCTTC TTCAGTAGCA GAGTCGGACA GTGAAGGCCC TCCCAAAATT
    GAGTTTGTGG ATAACCGAAT CAAGACTTTG GATGAGAAAT TGAGGACTCT GCTCTATCAG
    GAGCACGGCG GCAGTGGAGC AGCTGTGGGC AGTGCCTCCA CAGGAGGAAC TGCTCCAGCC
    TGCTCCACTT CAGGATCTGC CACAGCCTCT ACTGCAGTTG ATGAGTCTTC AGAAGTCCCC
    TACGCATTCT CCCTACCTCC TGCCACCTCC TCGGACACCT CCCCTCACTC CACCTCTTCC
    ACAACCTCCT CCACCACCCC TCGCTCTTCC TCCACCTCCC CGGATGGAGA GCGAGAGAAA
    GCAGGCGAGG AGACGACGAC TATCCCACCT GCGGTACAAC AACAGCCTGC CTCCTCTACA
    TCCCCTCCAT GCTCTCTTCT TTCCCATCCA CAGGAGCCTG GATCCCCCAA AGTTACTATT
    CAATCATCAG TAAGCGCTCC CCCTTCCCTC TCTGACAGCT GCACCCCTGG AGATGTTCTG
    TTGCCTTCCT CTTCTCAGCC GCCCGTCCCA CTGTGGCCTG GACAGCAGCA GCACAATGAA
    GGAGGTGGAT ATTTTGGCCT AAACCTGACA TGTCCTAGTA TCAGAAATCC TGTTAGCAAG
    AAATCCTGGA CTCGCAAATT CAAAAGCTGG GCATGCAAAC TGCGCCACTC CGCCAGCTTG
    TTCAAGAAGC CCAGAGTCCA GCAAGATGGC AACTCTAGCC AGGGGATGAA GGAGGCATCT
    ACAGATGACC AACCCTGCTC ACGCAGAGGC CGTTTCCAGG TGATTCCAGT ATCCCAGTCT
    GCAGAGACCC TTCCAACGCA GGAAGCTCCA CCAAGCCAAA TGATGAGAGT TGGCCGTTTC
    TCAGTCACTC AAGCTGAACA TCGAGAGGAG CATTTGACCG ACAGCTCCCC TGTGTCACCT
    GACCTGGAGC GAGAGAGGAG GAAAACGAAA GACGAGAGGA GAACGACAGC ACCCAGCCAC
    CCATCCAAGA GCCACGCGCA CTCACCCCTG GGCAGCAGTG ATGATGAGAG CGAGGTGGAG
    GATGAAGACT TAAGGAAAGA ACTGCACAAA CTCAGAGAGA AACACATCAA GGAGGTGGTG
    TCCCTGCAGG CACAGCAGAA CAGAGAACTA CAGGATTTGT ACAAGCAGCT CCGCTCTCTC
    AAAGACCACA GACAGCCAAT GTCTATGAGC CTGTCCCGCA CCTCTCCTCT GCCCTCCGCA
    CCCCTCGCTA TCTCCCCGCG CAGACCCCGA CCCGCCAAAG CCAAGCTCCG GCCTCGGCCG
    CACTCTCACA TGGATAACAA CGGAGTCGCA CACCAAGGGA GTCTTCAGCA GTCTAGCAGT
    TATTCCGGTG GGGAGCAAAG CCGGCTACAT TCACACTGCA AGCCAGAGAA ATCACAATCC
    CTGACATCTA ACAGAGACCA CAGTCCTTCA GCCCAGGGTT CTGGCAACAG AAAGAGCACA
    TTCACAGATG AGCTGCATAA ACTTGTCGAT GAGTGGTCAA AGGAAACTGT TTGTCCAGTT
    CAGCCCAAGC CTTCGCTGAA TCAGATTAAA CAGATCCAAC AAGTGCAGGA GTTGGGTGGA
    TGGAGCCAGC CTGCTGAGAC AACAGCTTCG TCGGGTTGGT TTTCCGGGGC CCCTTTAAGT
    ACGCCTGTAT CAGTCAGCAT GACCACCATG ACATCCACCC CCTATAGCGC CATTAGTGGA
    CAAACACAGC CGCCTCCGAC GCAATTCCCT ATCGTTCCTC TACCCCAGCA GAACGTGCAT
    CCACAACCCG GCCCGCACCG TCAGCCCCTG CAGTACGGCC CTTCACCCCT GCAGCAGGCC
    CAGCTCCCGG TGCCTGCAGC AGGACAAACA CCGCCCCCAA CCAATCCAGA CGCTGTATTC
    TGCTCATGCT CTTCCTCCTC CTGCTCCTCA TCCTCCTGTT CCACCTCCAG TGCCAAACTT
    CACCCCAAAC CCTCCACGTC CACCCTTCCT TTAGGACAGC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45153
    Clone ID Related Accession (Same CDS sequence) XM_009296927.2 , XM_021469968.1
    Accession Version XM_021469968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTC CCCTTCCACC TTACTCAGCA GATGTTGCAT CCTCACAAAT GTTACACTCC
    CGGCCTACTG ATGCCTCCAC TCCCCACAGT TCCCTAATAC AGTCACAGAC ATACATGCCC
    CCAGTATCGC CCCAGGCACC CATGGCTGTC CTTACGTCCT CTATTCCGAC TGGAGAACCA
    ATAGCACCAG CAGGCAACAT CATGCCACCC ATACAGACCC AAACTAGTAT TCCTCCTCTT
    CAACCCATGG ATGTTTTGCC CCTGCAAACT GTTGTTCAAA ATCAACAGCA AGTGATGCCT
    GAATTGCCCA GAACGCTGCA ACCTAACATC CAGCAACCAA CACCACAACA GCAGGCAAAT
    GTAATGCAGC AGCATCAGAC AGTCTTGTTG CCACAGCAAA CTGACCAGGT TCAGCAAATT
    AAAGTTTTGT TACAACAGTC ACAGCATGCA GAACAACCCC AGGTGGTTCT GCAGGAGCAG
    GCCCCACATT CCAGTGCATC TCAACAGCCA TATATCCCAT TACCACAGCA AGCACCGCCA
    ACAATGACCA ACCCACAGCA AAGTGAGCTG CAACAGCAGG TGTTTATACA GCAACCTCAA
    AGCAAACCCC AACAACAAGT TGGGATACAG CCTCAAGTTC AGCAGCATCA GACATCAATA
    CAGCAACCCT CAGTTGATCA GTATCAACAC CAGACTCAGC AACAGCAAGT GTATGTGCCA
    CAAATGGCAG AATCACAAGA CCAGAACGTG ATAAAGACTG TGATACAACA GGTACCGGTG
    CTGCAAGCAA TTCAGCAGCC ATTACAATTG ATAGAGCAAC AACAGCAGCA GCTTCTGATT
    AGGCAACAAA TTGAGCAACA TCAGAAGGCT GCTTTACAAA ACCAAATGGA GCAACATCAA
    CAGCAAGTTT ATATTCAGCC ACCACAACAG CAAAAAGTGG AACAACAACA AATTCTTATG
    CAGCAACAGC AAAACTTGGC AGTAAAGCAA CAGATAGAGC AACAGCAGCA TGCATTAAAG
    CAACAAAAGC AAATAGAGCA GCAGCAAAAA GCCATTTTAC AGCAACAGGC AGAACAGCAA
    AGACAGCAGC AACAGACTTT GTTTCAGCAA CAGCAGCTGG AGCAGCAACA AGTATTACTG
    CAGCAGCAAC AGCGTGAACA ACAACAGGCT CTTCTCCAGC AGCAACAGTT GGAGCAGCAA
    GCTTTACTGC AACAACAGCA GCAACTTCAG TTAAAGCAGC AACAAGCTCT TCTACATCAA
    CAACAGCTGG AGCAGCAGCA GCAGGCTTTG TTTAAACAGC AGCAGCTGGA GCAACAGCAA
    GCTCTTTTGC AGCAGCAGCA GCAGCAAGCT CTCGTTCAGC AGCAGCAACA GTTAGAGCAA
    AAAGTACTGC TACAGCTGCA ACAGCAACAG CAGCAACACA TCGATCAGCA GCAAAATTTA
    ATACGCCAAC AGCAGCCTCA GCAGATCGAG GCTGTTTTAC AGCAACAACC ACAACTTCAG
    AACTCAACTG ATCAACAATT GCAGCAGCAA ATATTTCAAC AACAGACCGA GCAGCAACAG
    ATGCAGCAGC ATCTACTGAT GAAACAACAG CAGATGGAAT TACAGCGACA ACAAGCTCTA
    CAAGCTGAGA AACAACGACA GCAAGTTCTA TTGCAACAAC AACATTTGGA GCAACATGCA
    GCTCTGGTGG ATCATAGTGG GCAGCAACAA GCAACCCTGG TGAAAGCACA GTCTCCAGAA
    ATGGTCCAGC AGATTAGTCA AGTGCAGCAA TCTCAACAAA TGATGTCTCT ACAACCGCAG
    CCTCATCTTG CTCAACAACA AACTGACCAG ACCCAGGTCT TGACTCAGCA ACAAGCCATT
    CTTCCCCAAG CAACACACAG ACCTTCTCTG GTAGACTTGC AAATCCCAAC CCTGCTTCAA
    GCAGCACCTA AAATAGTCAG CACTCAAATA CCTACCCAAG TTCAAGCCCC AGTACTTTTG
    CAGCAACAGG TGCCACCTCA GATGTCAGCT CAACCTACAG CACAAGTACA ACTCCAGAGT
    CAAGGTGAAC CAACAATTTA TGACCAGATT GCAATGCAGA GTCAAAGTCA TCCCGCACAG
    CAGTCAATGG GAGGTCAAAT TCAGACAGGC CAATTGCAAA TTCCACAATC AACCCAACAA
    ATCACATCCC CAACACAAGT TCCTACACCA CAACCCATTC ATTCTTTTGT TCCAACTTCA
    ACACAGATGG TCTCACAAGG GCAAACCCTT CCACCTCAAC CTCAACTGGT ACCCCCTCAA
    GCTGCAGCTT CTCTCCAGTG TCAAGTTGCA GTAGGGTCAT CTGCACCTGC ATCTTTTGTT
    CAAGCTACAG TCCCAACTGC TTCTACACTT GTGCCCACAC AGTATGCTCA AGCACCTGTT
    GCTTCTCAAA CGATCCAGTC TGTTCAGTTA GACCTCACTC AACCTGTGAA GCAGCAACAA
    CAACAACAGC CGCCTCTGCA GCAATATCAG CAAGCGGTGC TATCTCCGGT GTCACTTGCA
    CCTACGATCA ATCCAGTGGC TTCTTCCTCA ACACAGTGCT ATTTACAGCA GACAAACCAA
    TCACAAATTC AGGTCCAAAC CCAACAAGTA ATTCAAGCTC AGCAACAACC AGTCAGTTTG
    GCCTCGATGC AGGCATTCTT GCAGCCCCTA CCTTTAGTGC AGCCTCAGGC TACCCAACAG
    TATGAGCAGC ATTTGCCTCA AGCCCACCAC GTGCAACAAC AACCCCTCCA CCATCAGCAG
    CAGCTTCTGC CCCCAAATGC TGTTATACCA CAAGATATCC ACCAACAAAC GTGTCAGCCT
    CAACCAGTGC TCCAGCATGT GCTCCCATTA CACACTGCAC CCAGTCAGGT ACCTCAGTCC
    CAAGCTAGTA CAGTACCTCT CTACAGTCAG GTGGTGTCAG CTTCCCCCCA GCAGAAATTA
    ACTCTTCACA CTCAAACACA GTCTAGCATG CAGTCACAAG TGCTCTCAAA CTCACAGGCA
    CAGACTCACA TAGAGCCCAC TGGAGCATTT GAGCATCCTC ATTACACCCA GGCTGCCTTT
    ATGCCATCAC AGATTCCCAC AAGTCCATCT CATACTTCCA TACAGCCCTC TTCCACTCTC
    CCAGCCCAAC AACCAGTTGT CCCAGTTTCA GAAATTCCTG TCCCAGCAGA CGCACAGCTA
    ACACAGGCAA GGCCTGCTGA TTTGATCCCT GCTTCCACTC CTACTGTCAA TACTACCCAG
    TCCCATGACT CAAACGGCTC TGTGCTGACA ACCGCCATGC TGGCAGAAAG TGACGCTGCA
    TTGCTGGGCA TTGCTCAGGA AAGCCTAATT CACTCTGCCA ACAGGGGATC TTTATTATGC
    TCTGCTCCTA CCAATGGAGA TGAGGGCCAG TTGGTGTGGC CTAGTGGAAA ACTAGATAAG
    GCAAAATGTC AGAGGAGATC ATCCTGTCAG AAGACTGAAA AAACTCACTT CCAACTCTGC
    ATGTTGCAGG TCTCATGCAC AGGAGACAAC ATGGTAGAAT GTCAGTTGGA GACTCACAGC
    AACAAGATGG TCACGTTCAA GTTTGACATT GAGGGAGATG CACCTGAAGA CATTGCAGAT
    TACATGGTGG AGGAGGACTT CGTCCTTGAG CCTGAAAAAG AAAAATTTGT TGAGGAGCTG
    AGATGTATAG TGAAGAAAGC TCAAGCAATA TTAAGTACTC AGTCGCAGAC TGGATCCCTG
    GAGCAGCTCC AAGTGAGCAC CCCATCCAGT GCAACAATGG ATTCAGCCCC TCAGTCTTCT
    CCAGTAGGTC GCTGGCGGTT CTTCATTAAC CAGACTATTC GTCACCGTGA TTCACACTCC
    AACCAGGGAG CTTCGACTCC CCCTCCAGTG GGGGAGAACA GAGTTCCCAA GTCCATAGAA
    ACCGAGAAAG ATCATGAGAG CCCGCAGCGC TCCGAGTCTT TTGCTGGAAT AGCATCTTCC
    CCAAGTTCTT CACTATCTGC ATCCTCTCCT CCAGTCTCTA CTGTATCCGC TCCTGCATCC
    ATGTCAGCCT CAGCCATGGC TGCTCAAAAA GATTTTACAC CTGCTGCCTT GCCAGCAGTA
    GAGAAAGTCT CCTCTACTAT GCTGACCGCA GGTCCAGTCC CTGTCATGGC CTCCAGCACT
    TCTGCCACTC CTTTTCCTGC AGCCATGGCC CTCACAGGCT CTAATGTGGA CCAAGGAGAA
    TCTGCTGTTA AAGGTGAGGG TCAACAGCCT TATGTGACTC CTCCAACGTC ACTGTTGACC
    CATGCTGACC CATCTCAGCA CTTAATTGTT CAAACCCCAA TGGCACCCTC ACAGATGCCA
    GATATACCTC AACAAAGCCA GACACTTCAA TCAACATTGA TCACAACTGA ACAAGTTCAG
    CAACCGCAGA TTACCCCTCA GCCACTTCTG CAGGATGCTC CACAGCTACC TAAACCAGAA
    TCAAAATCGC TACCACAGGC AGTGCCTAAA GAGATGGTTC CTTTGCATGA AGGTCTTTCA
    GCTGCCCAAG TTCAGCAGGT CCCTCTCCAG CAAGTACCAG TAGAATTGCA ACAGCAGCCA
    ATGCAGCAGA CCACATTCTT ACAGCAAACA TCCAGTGCAG AGCAGATACA GCAGCACATG
    GCAGAACAGT TTTTATCTCA AGAAACCAAA TCAGCTCCAA TGATACTGAC CATTCATCCA
    CAGCATGGCT TCCAGACCCA GGATCCTCAG CAGGTTCAAG CTCCATTGCA GTCTATGATC
    CCCCAGCAGG CTCCAATTGA GCAAACCCAG ACATTATATA AACAGGTCCC ACATGATATG
    ATGCCAGTGG CAAAAACCGC AGTGCAGAAG CAAGTGTCTT TCCAGCAGTC AGAGTCTGAG
    CTGTCTACGG GTGAGGACAC CGTCAGCCAC TCTGCACTTC CGCATCCCTC TTTAGACACC
    TCTCTTCCAC CCCTCCCTCT CTCTGAAACA GCTCTTCCTG CCCTCTCCCA CACATTCACT
    CCTTCTCCAG CACAGCCTTC TTCAGTAGCA GAGTCGGACA GTGAAGGCCC TCCCAAAATT
    GAGTTTGTGG ATAACCGAAT CAAGACTTTG GATGAGAAAT TGAGGACTCT GCTCTATCAG
    GAGCACGGCG GCAGTGGAGC AGCTGTGGGC AGTGCCTCCA CAGGAGGAAC TGCTCCAGCC
    TGCTCCACTT CAGGATCTGC CACAGCCTCT ACTGCAGTTG ATGAGTCTTC AGAAGTCCCC
    TACGCATTCT CCCTACCTCC TGCCACCTCC TCGGACACCT CCCCTCACTC CACCTCTTCC
    ACAACCTCCT CCACCACCCC TCGCTCTTCC TCCACCTCCC CGGATGGAGA GCGAGAGAAA
    GCAGGCGAGG AGACGACGAC TATCCCACCT GCGGTACAAC AACAGCCTGC CTCCTCTACA
    TCCCCTCCAT GCTCTCTTCT TTCCCATCCA CAGGAGCCTG GATCCCCCAA AGTTACTATT
    CAATCATCAG TAAGCGCTCC CCCTTCCCTC TCTGACAGCT GCACCCCTGG AGATGTTCTG
    TTGCCTTCCT CTTCTCAGCC GCCCGTCCCA CTGTGGCCTG GACAGCAGCA GCACAATGAA
    GGAGGTGGAT ATTTTGGCCT AAACCTGACA TGTCCTAGTA TCAGAAATCC TGTTAGCAAG
    AAATCCTGGA CTCGCAAATT CAAAAGCTGG GCATGCAAAC TGCGCCACTC CGCCAGCTTG
    TTCAAGAAGC CCAGAGTCCA GCAAGATGGC AACTCTAGCC AGGGGATGAA GGAGGCATCT
    ACAGATGACC AACCCTGCTC ACGCAGAGGC CGTTTCCAGG TGATTCCAGT ATCCCAGTCT
    GCAGAGACCC TTCCAACGCA GGAAGCTCCA CCAAGCCAAA TGATGAGAGT TGGCCGTTTC
    TCAGTCACTC AAGCTGAACA TCGAGAGGAG CATTTGACCG ACAGCTCCCC TGTGTCACCT
    GACCTGGAGC GAGAGAGGAG GAAAACGAAA GACGAGAGGA GAACGACAGC ACCCAGCCAC
    CCATCCAAGA GCCACGCGCA CTCACCCCTG GGCAGCAGTG ATGATGAGAG CGAGGTGGAG
    GATGAAGACT TAAGGAAAGA ACTGCACAAA CTCAGAGAGA AACACATCAA GGAGGTGGTG
    TCCCTGCAGG CACAGCAGAA CAGAGAACTA CAGGATTTGT ACAAGCAGCT CCGCTCTCTC
    AAAGACCACA GACAGCCAAT GTCTATGAGC CTGTCCCGCA CCTCTCCTCT GCCCTCCGCA
    CCCCTCGCTA TCTCCCCGCG CAGACCCCGA CCCGCCAAAG CCAAGCTCCG GCCTCGGCCG
    CACTCTCACA TGGATAACAA CGGAGTCGCA CACCAAGGGA GTCTTCAGCA GTCTAGCAGT
    TATTCCGGTG GGGAGCAAAG CCGGCTACAT TCACACTGCA AGCCAGAGAA ATCACAATCC
    CTGACATCTA ACAGAGACCA CAGTCCTTCA GCCCAGGGTT CTGGCAACAG AAAGAGCACA
    TTCACAGATG AGCTGCATAA ACTTGTCGAT GAGTGGTCAA AGGAAACTGT TTGTCCAGTT
    CAGCCCAAGC CTTCGCTGAA TCAGATTAAA CAGATCCAAC AAGTGCAGGA GTTGGGTGGA
    TGGAGCCAGC CTGCTGAGAC AACAGCTTCG TCGGGTTGGT TTTCCGGGGC CCCTTTAAGT
    ACGCCTGTAT CAGTCAGCAT GACCACCATG ACATCCACCC CCTATAGCGC CATTAGTGGA
    CAAACACAGC CGCCTCCGAC GCAATTCCCT ATCGTTCCTC TACCCCAGCA GAACGTGCAT
    CCACAACCCG GCCCGCACCG TCAGCCCCTG CAGTACGGCC CTTCACCCCT GCAGCAGGCC
    CAGCTCCCGG TGCCTGCAGC AGGACAAACA CCGCCCCCAA CCAATCCAGA CGCTGTATTC
    TGCTCATGCT CTTCCTCCTC CTGCTCCTCA TCCTCCTGTT CCACCTCCAG TGCCAAACTT
    CACCCCAAAC CCTCCACGTC CACCCTTCCT TTAGGACAGC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325643.1
    CDS1064..10048
    Translation

    Target ORF information:

    RefSeq Version XM_021469968.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTC CCCTTCCACC TTACTCAGCA GATGTTGCAT CCTCACAAAT GTTACACTCC
    CGGCCTACTG ATGCCTCCAC TCCCCACAGT TCCCTAATAC AGTCACAGAC ATACATGCCC
    CCAGTATCGC CCCAGGCACC CATGGCTGTC CTTACGTCCT CTATTCCGAC TGGAGAACCA
    ATAGCACCAG CAGGCAACAT CATGCCACCC ATACAGACCC AAACTAGTAT TCCTCCTCTT
    CAACCCATGG ATGTTTTGCC CCTGCAAACT GTTGTTCAAA ATCAACAGCA AGTGATGCCT
    GAATTGCCCA GAACGCTGCA ACCTAACATC CAGCAACCAA CACCACAACA GCAGGCAAAT
    GTAATGCAGC AGCATCAGAC AGTCTTGTTG CCACAGCAAA CTGACCAGGT TCAGCAAATT
    AAAGTTTTGT TACAACAGTC ACAGCATGCA GAACAACCCC AGGTGGTTCT GCAGGAGCAG
    GCCCCACATT CCAGTGCATC TCAACAGCCA TATATCCCAT TACCACAGCA AGCACCGCCA
    ACAATGACCA ACCCACAGCA AAGTGAGCTG CAACAGCAGG TGTTTATACA GCAACCTCAA
    AGCAAACCCC AACAACAAGT TGGGATACAG CCTCAAGTTC AGCAGCATCA GACATCAATA
    CAGCAACCCT CAGTTGATCA GTATCAACAC CAGACTCAGC AACAGCAAGT GTATGTGCCA
    CAAATGGCAG AATCACAAGA CCAGAACGTG ATAAAGACTG TGATACAACA GGTACCGGTG
    CTGCAAGCAA TTCAGCAGCC ATTACAATTG ATAGAGCAAC AACAGCAGCA GCTTCTGATT
    AGGCAACAAA TTGAGCAACA TCAGAAGGCT GCTTTACAAA ACCAAATGGA GCAACATCAA
    CAGCAAGTTT ATATTCAGCC ACCACAACAG CAAAAAGTGG AACAACAACA AATTCTTATG
    CAGCAACAGC AAAACTTGGC AGTAAAGCAA CAGATAGAGC AACAGCAGCA TGCATTAAAG
    CAACAAAAGC AAATAGAGCA GCAGCAAAAA GCCATTTTAC AGCAACAGGC AGAACAGCAA
    AGACAGCAGC AACAGACTTT GTTTCAGCAA CAGCAGCTGG AGCAGCAACA AGTATTACTG
    CAGCAGCAAC AGCGTGAACA ACAACAGGCT CTTCTCCAGC AGCAACAGTT GGAGCAGCAA
    GCTTTACTGC AACAACAGCA GCAACTTCAG TTAAAGCAGC AACAAGCTCT TCTACATCAA
    CAACAGCTGG AGCAGCAGCA GCAGGCTTTG TTTAAACAGC AGCAGCTGGA GCAACAGCAA
    GCTCTTTTGC AGCAGCAGCA GCAGCAAGCT CTCGTTCAGC AGCAGCAACA GTTAGAGCAA
    AAAGTACTGC TACAGCTGCA ACAGCAACAG CAGCAACACA TCGATCAGCA GCAAAATTTA
    ATACGCCAAC AGCAGCCTCA GCAGATCGAG GCTGTTTTAC AGCAACAACC ACAACTTCAG
    AACTCAACTG ATCAACAATT GCAGCAGCAA ATATTTCAAC AACAGACCGA GCAGCAACAG
    ATGCAGCAGC ATCTACTGAT GAAACAACAG CAGATGGAAT TACAGCGACA ACAAGCTCTA
    CAAGCTGAGA AACAACGACA GCAAGTTCTA TTGCAACAAC AACATTTGGA GCAACATGCA
    GCTCTGGTGG ATCATAGTGG GCAGCAACAA GCAACCCTGG TGAAAGCACA GTCTCCAGAA
    ATGGTCCAGC AGATTAGTCA AGTGCAGCAA TCTCAACAAA TGATGTCTCT ACAACCGCAG
    CCTCATCTTG CTCAACAACA AACTGACCAG ACCCAGGTCT TGACTCAGCA ACAAGCCATT
    CTTCCCCAAG CAACACACAG ACCTTCTCTG GTAGACTTGC AAATCCCAAC CCTGCTTCAA
    GCAGCACCTA AAATAGTCAG CACTCAAATA CCTACCCAAG TTCAAGCCCC AGTACTTTTG
    CAGCAACAGG TGCCACCTCA GATGTCAGCT CAACCTACAG CACAAGTACA ACTCCAGAGT
    CAAGGTGAAC CAACAATTTA TGACCAGATT GCAATGCAGA GTCAAAGTCA TCCCGCACAG
    CAGTCAATGG GAGGTCAAAT TCAGACAGGC CAATTGCAAA TTCCACAATC AACCCAACAA
    ATCACATCCC CAACACAAGT TCCTACACCA CAACCCATTC ATTCTTTTGT TCCAACTTCA
    ACACAGATGG TCTCACAAGG GCAAACCCTT CCACCTCAAC CTCAACTGGT ACCCCCTCAA
    GCTGCAGCTT CTCTCCAGTG TCAAGTTGCA GTAGGGTCAT CTGCACCTGC ATCTTTTGTT
    CAAGCTACAG TCCCAACTGC TTCTACACTT GTGCCCACAC AGTATGCTCA AGCACCTGTT
    GCTTCTCAAA CGATCCAGTC TGTTCAGTTA GACCTCACTC AACCTGTGAA GCAGCAACAA
    CAACAACAGC CGCCTCTGCA GCAATATCAG CAAGCGGTGC TATCTCCGGT GTCACTTGCA
    CCTACGATCA ATCCAGTGGC TTCTTCCTCA ACACAGTGCT ATTTACAGCA GACAAACCAA
    TCACAAATTC AGGTCCAAAC CCAACAAGTA ATTCAAGCTC AGCAACAACC AGTCAGTTTG
    GCCTCGATGC AGGCATTCTT GCAGCCCCTA CCTTTAGTGC AGCCTCAGGC TACCCAACAG
    TATGAGCAGC ATTTGCCTCA AGCCCACCAC GTGCAACAAC AACCCCTCCA CCATCAGCAG
    CAGCTTCTGC CCCCAAATGC TGTTATACCA CAAGATATCC ACCAACAAAC GTGTCAGCCT
    CAACCAGTGC TCCAGCATGT GCTCCCATTA CACACTGCAC CCAGTCAGGT ACCTCAGTCC
    CAAGCTAGTA CAGTACCTCT CTACAGTCAG GTGGTGTCAG CTTCCCCCCA GCAGAAATTA
    ACTCTTCACA CTCAAACACA GTCTAGCATG CAGTCACAAG TGCTCTCAAA CTCACAGGCA
    CAGACTCACA TAGAGCCCAC TGGAGCATTT GAGCATCCTC ATTACACCCA GGCTGCCTTT
    ATGCCATCAC AGATTCCCAC AAGTCCATCT CATACTTCCA TACAGCCCTC TTCCACTCTC
    CCAGCCCAAC AACCAGTTGT CCCAGTTTCA GAAATTCCTG TCCCAGCAGA CGCACAGCTA
    ACACAGGCAA GGCCTGCTGA TTTGATCCCT GCTTCCACTC CTACTGTCAA TACTACCCAG
    TCCCATGACT CAAACGGCTC TGTGCTGACA ACCGCCATGC TGGCAGAAAG TGACGCTGCA
    TTGCTGGGCA TTGCTCAGGA AAGCCTAATT CACTCTGCCA ACAGGGGATC TTTATTATGC
    TCTGCTCCTA CCAATGGAGA TGAGGGCCAG TTGGTGTGGC CTAGTGGAAA ACTAGATAAG
    GCAAAATGTC AGAGGAGATC ATCCTGTCAG AAGACTGAAA AAACTCACTT CCAACTCTGC
    ATGTTGCAGG TCTCATGCAC AGGAGACAAC ATGGTAGAAT GTCAGTTGGA GACTCACAGC
    AACAAGATGG TCACGTTCAA GTTTGACATT GAGGGAGATG CACCTGAAGA CATTGCAGAT
    TACATGGTGG AGGAGGACTT CGTCCTTGAG CCTGAAAAAG AAAAATTTGT TGAGGAGCTG
    AGATGTATAG TGAAGAAAGC TCAAGCAATA TTAAGTACTC AGTCGCAGAC TGGATCCCTG
    GAGCAGCTCC AAGTGAGCAC CCCATCCAGT GCAACAATGG ATTCAGCCCC TCAGTCTTCT
    CCAGTAGGTC GCTGGCGGTT CTTCATTAAC CAGACTATTC GTCACCGTGA TTCACACTCC
    AACCAGGGAG CTTCGACTCC CCCTCCAGTG GGGGAGAACA GAGTTCCCAA GTCCATAGAA
    ACCGAGAAAG ATCATGAGAG CCCGCAGCGC TCCGAGTCTT TTGCTGGAAT AGCATCTTCC
    CCAAGTTCTT CACTATCTGC ATCCTCTCCT CCAGTCTCTA CTGTATCCGC TCCTGCATCC
    ATGTCAGCCT CAGCCATGGC TGCTCAAAAA GATTTTACAC CTGCTGCCTT GCCAGCAGTA
    GAGAAAGTCT CCTCTACTAT GCTGACCGCA GGTCCAGTCC CTGTCATGGC CTCCAGCACT
    TCTGCCACTC CTTTTCCTGC AGCCATGGCC CTCACAGGCT CTAATGTGGA CCAAGGAGAA
    TCTGCTGTTA AAGGTGAGGG TCAACAGCCT TATGTGACTC CTCCAACGTC ACTGTTGACC
    CATGCTGACC CATCTCAGCA CTTAATTGTT CAAACCCCAA TGGCACCCTC ACAGATGCCA
    GATATACCTC AACAAAGCCA GACACTTCAA TCAACATTGA TCACAACTGA ACAAGTTCAG
    CAACCGCAGA TTACCCCTCA GCCACTTCTG CAGGATGCTC CACAGCTACC TAAACCAGAA
    TCAAAATCGC TACCACAGGC AGTGCCTAAA GAGATGGTTC CTTTGCATGA AGGTCTTTCA
    GCTGCCCAAG TTCAGCAGGT CCCTCTCCAG CAAGTACCAG TAGAATTGCA ACAGCAGCCA
    ATGCAGCAGA CCACATTCTT ACAGCAAACA TCCAGTGCAG AGCAGATACA GCAGCACATG
    GCAGAACAGT TTTTATCTCA AGAAACCAAA TCAGCTCCAA TGATACTGAC CATTCATCCA
    CAGCATGGCT TCCAGACCCA GGATCCTCAG CAGGTTCAAG CTCCATTGCA GTCTATGATC
    CCCCAGCAGG CTCCAATTGA GCAAACCCAG ACATTATATA AACAGGTCCC ACATGATATG
    ATGCCAGTGG CAAAAACCGC AGTGCAGAAG CAAGTGTCTT TCCAGCAGTC AGAGTCTGAG
    CTGTCTACGG GTGAGGACAC CGTCAGCCAC TCTGCACTTC CGCATCCCTC TTTAGACACC
    TCTCTTCCAC CCCTCCCTCT CTCTGAAACA GCTCTTCCTG CCCTCTCCCA CACATTCACT
    CCTTCTCCAG CACAGCCTTC TTCAGTAGCA GAGTCGGACA GTGAAGGCCC TCCCAAAATT
    GAGTTTGTGG ATAACCGAAT CAAGACTTTG GATGAGAAAT TGAGGACTCT GCTCTATCAG
    GAGCACGGCG GCAGTGGAGC AGCTGTGGGC AGTGCCTCCA CAGGAGGAAC TGCTCCAGCC
    TGCTCCACTT CAGGATCTGC CACAGCCTCT ACTGCAGTTG ATGAGTCTTC AGAAGTCCCC
    TACGCATTCT CCCTACCTCC TGCCACCTCC TCGGACACCT CCCCTCACTC CACCTCTTCC
    ACAACCTCCT CCACCACCCC TCGCTCTTCC TCCACCTCCC CGGATGGAGA GCGAGAGAAA
    GCAGGCGAGG AGACGACGAC TATCCCACCT GCGGTACAAC AACAGCCTGC CTCCTCTACA
    TCCCCTCCAT GCTCTCTTCT TTCCCATCCA CAGGAGCCTG GATCCCCCAA AGTTACTATT
    CAATCATCAG TAAGCGCTCC CCCTTCCCTC TCTGACAGCT GCACCCCTGG AGATGTTCTG
    TTGCCTTCCT CTTCTCAGCC GCCCGTCCCA CTGTGGCCTG GACAGCAGCA GCACAATGAA
    GGAGGTGGAT ATTTTGGCCT AAACCTGACA TGTCCTAGTA TCAGAAATCC TGTTAGCAAG
    AAATCCTGGA CTCGCAAATT CAAAAGCTGG GCATGCAAAC TGCGCCACTC CGCCAGCTTG
    TTCAAGAAGC CCAGAGTCCA GCAAGATGGC AACTCTAGCC AGGGGATGAA GGAGGCATCT
    ACAGATGACC AACCCTGCTC ACGCAGAGGC CGTTTCCAGG TGATTCCAGT ATCCCAGTCT
    GCAGAGACCC TTCCAACGCA GGAAGCTCCA CCAAGCCAAA TGATGAGAGT TGGCCGTTTC
    TCAGTCACTC AAGCTGAACA TCGAGAGGAG CATTTGACCG ACAGCTCCCC TGTGTCACCT
    GACCTGGAGC GAGAGAGGAG GAAAACGAAA GACGAGAGGA GAACGACAGC ACCCAGCCAC
    CCATCCAAGA GCCACGCGCA CTCACCCCTG GGCAGCAGTG ATGATGAGAG CGAGGTGGAG
    GATGAAGACT TAAGGAAAGA ACTGCACAAA CTCAGAGAGA AACACATCAA GGAGGTGGTG
    TCCCTGCAGG CACAGCAGAA CAGAGAACTA CAGGATTTGT ACAAGCAGCT CCGCTCTCTC
    AAAGACCACA GACAGCCAAT GTCTATGAGC CTGTCCCGCA CCTCTCCTCT GCCCTCCGCA
    CCCCTCGCTA TCTCCCCGCG CAGACCCCGA CCCGCCAAAG CCAAGCTCCG GCCTCGGCCG
    CACTCTCACA TGGATAACAA CGGAGTCGCA CACCAAGGGA GTCTTCAGCA GTCTAGCAGT
    TATTCCGGTG GGGAGCAAAG CCGGCTACAT TCACACTGCA AGCCAGAGAA ATCACAATCC
    CTGACATCTA ACAGAGACCA CAGTCCTTCA GCCCAGGGTT CTGGCAACAG AAAGAGCACA
    TTCACAGATG AGCTGCATAA ACTTGTCGAT GAGTGGTCAA AGGAAACTGT TTGTCCAGTT
    CAGCCCAAGC CTTCGCTGAA TCAGATTAAA CAGATCCAAC AAGTGCAGGA GTTGGGTGGA
    TGGAGCCAGC CTGCTGAGAC AACAGCTTCG TCGGGTTGGT TTTCCGGGGC CCCTTTAAGT
    ACGCCTGTAT CAGTCAGCAT GACCACCATG ACATCCACCC CCTATAGCGC CATTAGTGGA
    CAAACACAGC CGCCTCCGAC GCAATTCCCT ATCGTTCCTC TACCCCAGCA GAACGTGCAT
    CCACAACCCG GCCCGCACCG TCAGCCCCTG CAGTACGGCC CTTCACCCCT GCAGCAGGCC
    CAGCTCCCGG TGCCTGCAGC AGGACAAACA CCGCCCCCAA CCAATCCAGA CGCTGTATTC
    TGCTCATGCT CTTCCTCCTC CTGCTCCTCA TCCTCCTGTT CCACCTCCAG TGCCAAACTT
    CACCCCAAAC CCTCCACGTC CACCCTTCCT TTAGGACAGC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45154
    Clone ID Related Accession (Same CDS sequence) XM_009296928.2 , XM_021469969.1
    Accession Version XM_009296928.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
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    3121
    3181
    3241
    3301
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    3421
    3481
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    4021
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    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
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    4921
    4981
    5041
    5101
    5161
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    5341
    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GGGGTAAGAA CAGACGAGGG TAGAAAAGGG
    AGAGAATCTA AGAGGGATGA CCCTGGATCT GAGTCTCCAG TCTCACCGTA TGCAGTGCTG
    TTATTGCGGT GCCTGCACCC CTCACCATCC TGCACCCGCC TCACTCACCC ACAGCACCTG
    AAATGGCTAA AATGGCACAC TTGTTTAAAA ACTACATGGG CTGGACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295203.1
    CDS996..9086
    Translation

    Target ORF information:

    RefSeq Version XM_009296928.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio im:7152756 (im:7152756), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296928.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GGGGTAAGAA CAGACGAGGG TAGAAAAGGG
    AGAGAATCTA AGAGGGATGA CCCTGGATCT GAGTCTCCAG TCTCACCGTA TGCAGTGCTG
    TTATTGCGGT GCCTGCACCC CTCACCATCC TGCACCCGCC TCACTCACCC ACAGCACCTG
    AAATGGCTAA AATGGCACAC TTGTTTAAAA ACTACATGGG CTGGACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45154
    Clone ID Related Accession (Same CDS sequence) XM_009296928.2 , XM_021469969.1
    Accession Version XM_021469969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GGGGTAAGAA CAGACGAGGG TAGAAAAGGG
    AGAGAATCTA AGAGGGATGA CCCTGGATCT GAGTCTCCAG TCTCACCGTA TGCAGTGCTG
    TTATTGCGGT GCCTGCACCC CTCACCATCC TGCACCCGCC TCACTCACCC ACAGCACCTG
    AAATGGCTAA AATGGCACAC TTGTTTAAAA ACTACATGGG CTGGACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325644.1
    CDS1064..9154
    Translation

    Target ORF information:

    RefSeq Version XM_021469969.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGCCCCT TCCACCTTAC TCAGCAGATG TTGCATCCTC ACAAATGTTA
    CACTCCCGGC CTACTGATGC CTCCACTCCC CACAGTTCCC TAATACAGTC ACAGACATAC
    ATGCCCCCAG TATCGCCCCA GGCACCCATG GCTGTCCTTA CGTCCTCTAT TCCGACTGGA
    GAACCAATAG CACCAGCAGG CAACATCATG CCACCCATAC AGACCCAAAC TAGTATTCCT
    CCTCTTCAAC CCATGGATGT TTTGCCCCTG CAAACTGTTG TTCAAAATCA ACAGCAAGTG
    ATGCCTGAAT TGCCCAGAAC GCTGCAACCT AACATCCAGC AACCAACACC ACAACAGCAG
    GCAAATGTAA TGCAGCAGCA TCAGACAGTC TTGTTGCCAC AGCAAACTGA CCAGGTTCAG
    CAAATTAAAG TTTTGTTACA ACAGTCACAG CATGCAGAAC AACCCCAGGT GGTTCTGCAG
    GAGCAGGCCC CACATTCCAG TGCATCTCAA CAGCCATATA TCCCATTACC ACAGCAAGCA
    CCGCCAACAA TGACCAACCC ACAGCAAAGT GAGCTGCAAC AGCAGGTGTT TATACAGCAA
    CCTCAAAGCA AACCCCAACA ACAAGTTGGG ATACAGCCTC AAGTTCAGCA GCATCAGACA
    TCAATACAGC AACCCTCAGT TGATCAGTAT CAACACCAGA CTCAGCAACA GCAAGTGTAT
    GTGCCACAAA TGGCAGAATC ACAAGACCAG AACGTGATAA AGACTGTGAT ACAACAGGTA
    CCGGTGCTGC AAGCAATTCA GCAGCCATTA CAATTGATAG AGCAACAACA GCAGCAGCTT
    CTGATTAGGC AACAAATTGA GCAACATCAG AAGGCTGCTT TACAAAACCA AATGGAGCAA
    CATCAACAGC AAGTTTATAT TCAGCCACCA CAACAGCAAA AAGTGGAACA ACAACAAATT
    CTTATGCAGC AACAGCAAAA CTTGGCAGTA AAGCAACAGA TAGAGCAACA GCAGCATGCA
    TTAAAGCAAC AAAAGCAAAT AGAGCAGCAG CAAAAAGCCA TTTTACAGCA ACAGGCAGAA
    CAGCAAAGAC AGCAGCAACA GACTTTGTTT CAGCAACAGC AGCTGGAGCA GCAACAAGTA
    TTACTGCAGC AGCAACAGCG TGAACAACAA CAGGCTCTTC TCCAGCAGCA ACAGTTGGAG
    CAGCAAGCTT TACTGCAACA ACAGCAGCAA CTTCAGTTAA AGCAGCAACA AGCTCTTCTA
    CATCAACAAC AGCTGGAGCA GCAGCAGCAG GCTTTGTTTA AACAGCAGCA GCTGGAGCAA
    CAGCAAGCTC TTTTGCAGCA GCAGCAGCAG CAAGCTCTCG TTCAGCAGCA GCAACAGTTA
    GAGCAAAAAG TACTGCTACA GCTGCAACAG CAACAGCAGC AACACATCGA TCAGCAGCAA
    AATTTAATAC GCCAACAGCA GCCTCAGCAG ATCGAGGCTG TTTTACAGCA ACAACCACAA
    CTTCAGAACT CAACTGATCA ACAATTGCAG CAGCAAATAT TTCAACAACA GACCGAGCAG
    CAACAGATGC AGCAGCATCT ACTGATGAAA CAACAGCAGA TGGAATTACA GCGACAACAA
    GCTCTACAAG CTGAGAAACA ACGACAGCAA GTTCTATTGC AACAACAACA TTTGGAGCAA
    CATGCAGCTC TGGTGGATCA TAGTGGGCAG CAACAAGCAA CCCTGGTGAA AGCACAGTCT
    CCAGAAATGG TCCAGCAGAT TAGTCAAGTG CAGCAATCTC AACAAATGAT GTCTCTACAA
    CCGCAGCCTC ATCTTGCTCA ACAACAAACT GACCAGACCC AGGTCTTGAC TCAGCAACAA
    GCCATTCTTC CCCAAGCAAC ACACAGACCT TCTCTGGTAG ACTTGCAAAT CCCAACCCTG
    CTTCAAGCAG CACCTAAAAT AGTCAGCACT CAAATACCTA CCCAAGTTCA AGCCCCAGTA
    CTTTTGCAGC AACAGGTGCC ACCTCAGATG TCAGCTCAAC CTACAGCACA AGTACAACTC
    CAGAGTCAAG GTGAACCAAC AATTTATGAC CAGATTGCAA TGCAGAGTCA AAGTCATCCC
    GCACAGCAGT CAATGGGAGG TCAAATTCAG ACAGGCCAAT TGCAAATTCC ACAATCAACC
    CAACAAATCA CATCCCCAAC ACAAGTTCCT ACACCACAAC CCATTCATTC TTTTGTTCCA
    ACTTCAACAC AGATGGTCTC ACAAGGGCAA ACCCTTCCAC CTCAACCTCA ACTGGTACCC
    CCTCAAGCTG CAGCTTCTCT CCAGTGTCAA GTTGCAGTAG GGTCATCTGC ACCTGCATCT
    TTTGTTCAAG CTACAGTCCC AACTGCTTCT ACACTTGTGC CCACACAGTA TGCTCAAGCA
    CCTGTTGCTT CTCAAACGAT CCAGTCTGTT CAGTTAGACC TCACTCAACC TGTGAAGCAG
    CAACAACAAC AACAGCCGCC TCTGCAGCAA TATCAGCAAG CGGTGCTATC TCCGGTGTCA
    CTTGCACCTA CGATCAATCC AGTGGCTTCT TCCTCAACAC AGTGCTATTT ACAGCAGACA
    AACCAATCAC AAATTCAGGT CCAAACCCAA CAAGTAATTC AAGCTCAGCA ACAACCAGTC
    AGTTTGGCCT CGATGCAGGC ATTCTTGCAG CCCCTACCTT TAGTGCAGCC TCAGGCTACC
    CAACAGTATG AGCAGCATTT GCCTCAAGCC CACCACGTGC AACAACAACC CCTCCACCAT
    CAGCAGCAGC TTCTGCCCCC AAATGCTGTT ATACCACAAG ATATCCACCA ACAAACGTGT
    CAGCCTCAAC CAGTGCTCCA GCATGTGCTC CCATTACACA CTGCACCCAG TCAGGTACCT
    CAGTCCCAAG CTAGTACAGT ACCTCTCTAC AGTCAGGTGG TGTCAGCTTC CCCCCAGCAG
    AAATTAACTC TTCACACTCA AACACAGTCT AGCATGCAGT CACAAGTGCT CTCAAACTCA
    CAGGCACAGA CTCACATAGA GCCCACTGGA GCATTTGAGC ATCCTCATTA CACCCAGGCT
    GCCTTTATGC CATCACAGAT TCCCACAAGT CCATCTCATA CTTCCATACA GCCCTCTTCC
    ACTCTCCCAG CCCAACAACC AGTTGTCCCA GTTTCAGAAA TTCCTGTCCC AGCAGACGCA
    CAGCTAACAC AGGCAAGGCC TGCTGATTTG ATCCCTGCTT CCACTCCTAC TGTCAATACT
    ACCCAGTCCC ATGACTCAAA CGGCTCTGTG CTGACAACCG CCATGCTGGC AGAAAGTGAC
    GCTGCATTGC TGGGCATTGC TCAGGAAAGC CTAATTCACT CTGCCAACAG GGGATCTTTA
    TTATGCTCTG CTCCTACCAA TGGAGATGAG GGCCAGTTGG TGTGGCCTAG TGGAAAACTA
    GATAAGGCAA AATGTCAGAG GAGATCATCC TGTCAGAAGA CTGAAAAAAC TCACTTCCAA
    CTCTGCATGT TGCAGGTCTC ATGCACAGGA GACAACATGG TAGAATGTCA GTTGGAGACT
    CACAGCAACA AGATGGTCAC GTTCAAGTTT GACATTGAGG GAGATGCACC TGAAGACATT
    GCAGATTACA TGGTGGAGGA GGACTTCGTC CTTGAGCCTG AAAAAGAAAA ATTTGTTGAG
    GAGCTGAGAT GTATAGTGAA GAAAGCTCAA GCAATATTAA GTACTCAGTC GCAGACTGGA
    TCCCTGGAGC AGCTCCAAGT GAGCACCCCA TCCAGTGCAA CAATGGATTC AGCCCCTCAG
    TCTTCTCCAG TAGGTCGCTG GCGGTTCTTC ATTAACCAGA CTATTCGTCA CCGTGATTCA
    CACTCCAACC AGGGAGCTTC GACTCCCCCT CCAGTGGGGG AGAACAGAGT TCCCAAGTCC
    ATAGAAACCG AGAAAGATCA TGAGAGCCCG CAGCGCTCCG AGTCTTTTGC TGGAATAGCA
    TCTTCCCCAA GTTCTTCACT ATCTGCATCC TCTCCTCCAG TCTCTACTGT ATCCGCTCCT
    GCATCCATGT CAGCCTCAGC CATGGCTGCT CAAAAAGATT TTACACCTGC TGCCTTGCCA
    GCAGTAGAGA AAGTCTCCTC TACTATGCTG ACCGCAGGTC CAGTCCCTGT CATGGCCTCC
    AGCACTTCTG CCACTCCTTT TCCTGCAGCC ATGGCCCTCA CAGGCTCTAA TGTGGACCAA
    GGAGAATCTG CTGTTAAAGG TGAGGGTCAA CAGCCTTATG TGACTCCTCC AACGTCACTG
    TTGACCCATG CTGACCCATC TCAGCACTTA ATTGTTCAAA CCCCAATGGC ACCCTCACAG
    ATGCCAGATA TACCTCAACA AAGCCAGACA CTTCAATCAA CATTGATCAC AACTGAACAA
    GTTCAGCAAC CGCAGATTAC CCCTCAGCCA CTTCTGCAGG ATGCTCCACA GCTACCTAAA
    CCAGAATCAA AATCGCTACC ACAGGCAGTG CCTAAAGAGA TGGTTCCTTT GCATGAAGGT
    CTTTCAGCTG CCCAAGTTCA GCAGGTCCCT CTCCAGCAAG TACCAGTAGA ATTGCAACAG
    CAGCCAATGC AGCAGACCAC ATTCTTACAG CAAACATCCA GTGCAGAGCA GATACAGCAG
    CACATGGCAG AACAGTTTTT ATCTCAAGAA ACCAAATCAG CTCCAATGAT ACTGACCATT
    CATCCACAGC ATGGCTTCCA GACCCAGGAT CCTCAGCAGG TTCAAGCTCC ATTGCAGTCT
    ATGATCCCCC AGCAGGCTCC AATTGAGCAA ACCCAGACAT TATATAAACA GGTCCCACAT
    GATATGATGC CAGTGGCAAA AACCGCAGTG CAGAAGCAAG TGTCTTTCCA GCAGTCAGAG
    TCTGAGCTGT CTACGGGTGA GGACACCGTC AGCCACTCTG CACTTCCGCA TCCCTCTTTA
    GACACCTCTC TTCCACCCCT CCCTCTCTCT GAAACAGCTC TTCCTGCCCT CTCCCACACA
    TTCACTCCTT CTCCAGCACA GCCTTCTTCA GTAGCAGAGT CGGACAGTGA AGGCCCTCCC
    AAAATTGAGT TTGTGGATAA CCGAATCAAG ACTTTGGATG AGAAATTGAG GACTCTGCTC
    TATCAGGAGC ACGGCGGCAG TGGAGCAGCT GTGGGCAGTG CCTCCACAGG AGGAACTGCT
    CCAGCCTGCT CCACTTCAGG ATCTGCCACA GCCTCTACTG CAGTTGATGA GTCTTCAGAA
    GTCCCCTACG CATTCTCCCT ACCTCCTGCC ACCTCCTCGG ACACCTCCCC TCACTCCACC
    TCTTCCACAA CCTCCTCCAC CACCCCTCGC TCTTCCTCCA CCTCCCCGGA TGGAGAGCGA
    GAGAAAGCAG GCGAGGAGAC GACGACTATC CCACCTGCGG TACAACAACA GCCTGCCTCC
    TCTACATCCC CTCCATGCTC TCTTCTTTCC CATCCACAGG AGCCTGGATC CCCCAAAGTT
    ACTATTCAAT CATCAGTAAG CGCTCCCCCT TCCCTCTCTG ACAGCTGCAC CCCTGGAGAT
    GTTCTGTTGC CTTCCTCTTC TCAGCCGCCC GTCCCACTGT GGCCTGGACA GCAGCAGCAC
    AATGAAGGAG GTGGATATTT TGGCCTAAAC CTGACATGTC CTAGTATCAG AAATCCTGTT
    AGCAAGAAAT CCTGGACTCG CAAATTCAAA AGCTGGGCAT GCAAACTGCG CCACTCCGCC
    AGCTTGTTCA AGAAGCCCAG AGTCCAGCAA GGGGTAAGAA CAGACGAGGG TAGAAAAGGG
    AGAGAATCTA AGAGGGATGA CCCTGGATCT GAGTCTCCAG TCTCACCGTA TGCAGTGCTG
    TTATTGCGGT GCCTGCACCC CTCACCATCC TGCACCCGCC TCACTCACCC ACAGCACCTG
    AAATGGCTAA AATGGCACAC TTGTTTAAAA ACTACATGGG CTGGACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45155
    Clone ID Related Accession (Same CDS sequence) XM_009296929.2 , XM_021469970.1
    Accession Version XM_009296929.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGTC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACATTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TGTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC ACACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTTACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCCAATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GATGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATTGCAACA GCAGCCAATG
    CAGCAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATACAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGACCAT TCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TATGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGGTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGT CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACT GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCCAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGATGGCAAC TCTAGCCAGG GGATGAAGGA GGCATCTACA
    GATGACCAAC CCTGCTCACG CAGAGGCCGT TTCCAGGTGA TTCCAGTATC CCAGTCTGCA
    GAGACCCTTC CAACGCAGGA AGCTCCACCA AGCCAAATGA TGAGAGTTGG CCGTTTCTCA
    GTCACTCAAG CTGAACATCG AGAGGAGCAT TTGACCGACA GCTCCCCTGT GTCACCTGAC
    CTGGAGCGAG AGAGGAGGAA AACGAAAGAC GAGAGGAGAA CGACAGCACC CAGCCACCCA
    TCCAAGAGCC ACGCGCACTC ACCCCTGGGC AGCAGTGATG ATGAGAGCGA GGTGGAGGAT
    GAAGACTTAA GGAAAGAACT GCACAAACTC AGAGAGAAAC ACATCAAGGA GGTGGTGTCC
    CTGCAGGCAC AGCAGAACAG AGAACTACAG GATTTGTACA AGCAGCTCCG CTCTCTCAAA
    GACCACAGAC AGCCAATGTC TATGAGCCTG TCCCGCACCT CTCCTCTGCC CTCCGCACCC
    CTCGCTATCT CCCCGCGCAG ACCCCGACCC GCCAAAGCCA AGCTCCGGCC TCGGCCGCAC
    TCTCACATGG ATAACAACGG AGTCGCACAC CAAGGGAGTC TTCAGCAGTC TAGCAGTTAT
    TCCGGTGGGG AGCAAAGCCG GCTACATTCA CACTGCAAGC CAGAGAAATC ACAATCCCTG
    ACATCTAACA GAGACCACAG TCCTTCAGCC CAGGGTTCTG GCAACAGAAA GAGCACATTC
    ACAGATGAGC TGCATAAACT TGTCGATGAG TGGTCAAAGG AAACTGTTTG TCCAGTTCAG
    CCCAAGCCTT CGCTGAATCA GATTAAACAG ATCCAACAAG TGCAGGAGTT GGGTGGATGG
    AGCCAGCCTG CTGAGACAAC AGCTTCGTCG GGTTGGTTTT CCGGGGCCCC TTTAAGTACG
    CCTGTATCAG TCAGCATGAC CACCATGACA TCCACCCCCT ATAGCGCCAT TAGTGGACAA
    ACACAGCCGC CTCCGACGCA ATTCCCTATC GTTCCTCTAC CCCAGCAGAA CGTGCATCCA
    CAACCCGGCC CGCACCGTCA GCCCCTGCAG TACGGCCCTT CACCCCTGCA GCAGGCCCAG
    CTCCCGGTGC CTGCAGCAGG ACAAACACCG CCCCCAACCA ATCCAGACGC TGTATTCTGC
    TCATGCTCTT CCTCCTCCTG CTCCTCATCC TCCTGTTCCA CCTCCAGTGC CAAACTTCAC
    CCCAAACCCT CCACGTCCAC CCTTCCTTTA GGACAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295204.1
    CDS996..6857
    Translation

    Target ORF information:

    RefSeq Version XM_009296929.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio im:7152756 (im:7152756), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296929.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGTC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACATTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TGTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC ACACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTTACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCCAATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GATGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATTGCAACA GCAGCCAATG
    CAGCAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATACAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGACCAT TCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TATGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGGTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGT CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACT GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCCAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGATGGCAAC TCTAGCCAGG GGATGAAGGA GGCATCTACA
    GATGACCAAC CCTGCTCACG CAGAGGCCGT TTCCAGGTGA TTCCAGTATC CCAGTCTGCA
    GAGACCCTTC CAACGCAGGA AGCTCCACCA AGCCAAATGA TGAGAGTTGG CCGTTTCTCA
    GTCACTCAAG CTGAACATCG AGAGGAGCAT TTGACCGACA GCTCCCCTGT GTCACCTGAC
    CTGGAGCGAG AGAGGAGGAA AACGAAAGAC GAGAGGAGAA CGACAGCACC CAGCCACCCA
    TCCAAGAGCC ACGCGCACTC ACCCCTGGGC AGCAGTGATG ATGAGAGCGA GGTGGAGGAT
    GAAGACTTAA GGAAAGAACT GCACAAACTC AGAGAGAAAC ACATCAAGGA GGTGGTGTCC
    CTGCAGGCAC AGCAGAACAG AGAACTACAG GATTTGTACA AGCAGCTCCG CTCTCTCAAA
    GACCACAGAC AGCCAATGTC TATGAGCCTG TCCCGCACCT CTCCTCTGCC CTCCGCACCC
    CTCGCTATCT CCCCGCGCAG ACCCCGACCC GCCAAAGCCA AGCTCCGGCC TCGGCCGCAC
    TCTCACATGG ATAACAACGG AGTCGCACAC CAAGGGAGTC TTCAGCAGTC TAGCAGTTAT
    TCCGGTGGGG AGCAAAGCCG GCTACATTCA CACTGCAAGC CAGAGAAATC ACAATCCCTG
    ACATCTAACA GAGACCACAG TCCTTCAGCC CAGGGTTCTG GCAACAGAAA GAGCACATTC
    ACAGATGAGC TGCATAAACT TGTCGATGAG TGGTCAAAGG AAACTGTTTG TCCAGTTCAG
    CCCAAGCCTT CGCTGAATCA GATTAAACAG ATCCAACAAG TGCAGGAGTT GGGTGGATGG
    AGCCAGCCTG CTGAGACAAC AGCTTCGTCG GGTTGGTTTT CCGGGGCCCC TTTAAGTACG
    CCTGTATCAG TCAGCATGAC CACCATGACA TCCACCCCCT ATAGCGCCAT TAGTGGACAA
    ACACAGCCGC CTCCGACGCA ATTCCCTATC GTTCCTCTAC CCCAGCAGAA CGTGCATCCA
    CAACCCGGCC CGCACCGTCA GCCCCTGCAG TACGGCCCTT CACCCCTGCA GCAGGCCCAG
    CTCCCGGTGC CTGCAGCAGG ACAAACACCG CCCCCAACCA ATCCAGACGC TGTATTCTGC
    TCATGCTCTT CCTCCTCCTG CTCCTCATCC TCCTGTTCCA CCTCCAGTGC CAAACTTCAC
    CCCAAACCCT CCACGTCCAC CCTTCCTTTA GGACAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45155
    Clone ID Related Accession (Same CDS sequence) XM_009296929.2 , XM_021469970.1
    Accession Version XM_021469970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein kinase WNK3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGTC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACATTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TGTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC ACACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTTACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCCAATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GATGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATTGCAACA GCAGCCAATG
    CAGCAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATACAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGACCAT TCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TATGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGGTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGT CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACT GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCCAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGATGGCAAC TCTAGCCAGG GGATGAAGGA GGCATCTACA
    GATGACCAAC CCTGCTCACG CAGAGGCCGT TTCCAGGTGA TTCCAGTATC CCAGTCTGCA
    GAGACCCTTC CAACGCAGGA AGCTCCACCA AGCCAAATGA TGAGAGTTGG CCGTTTCTCA
    GTCACTCAAG CTGAACATCG AGAGGAGCAT TTGACCGACA GCTCCCCTGT GTCACCTGAC
    CTGGAGCGAG AGAGGAGGAA AACGAAAGAC GAGAGGAGAA CGACAGCACC CAGCCACCCA
    TCCAAGAGCC ACGCGCACTC ACCCCTGGGC AGCAGTGATG ATGAGAGCGA GGTGGAGGAT
    GAAGACTTAA GGAAAGAACT GCACAAACTC AGAGAGAAAC ACATCAAGGA GGTGGTGTCC
    CTGCAGGCAC AGCAGAACAG AGAACTACAG GATTTGTACA AGCAGCTCCG CTCTCTCAAA
    GACCACAGAC AGCCAATGTC TATGAGCCTG TCCCGCACCT CTCCTCTGCC CTCCGCACCC
    CTCGCTATCT CCCCGCGCAG ACCCCGACCC GCCAAAGCCA AGCTCCGGCC TCGGCCGCAC
    TCTCACATGG ATAACAACGG AGTCGCACAC CAAGGGAGTC TTCAGCAGTC TAGCAGTTAT
    TCCGGTGGGG AGCAAAGCCG GCTACATTCA CACTGCAAGC CAGAGAAATC ACAATCCCTG
    ACATCTAACA GAGACCACAG TCCTTCAGCC CAGGGTTCTG GCAACAGAAA GAGCACATTC
    ACAGATGAGC TGCATAAACT TGTCGATGAG TGGTCAAAGG AAACTGTTTG TCCAGTTCAG
    CCCAAGCCTT CGCTGAATCA GATTAAACAG ATCCAACAAG TGCAGGAGTT GGGTGGATGG
    AGCCAGCCTG CTGAGACAAC AGCTTCGTCG GGTTGGTTTT CCGGGGCCCC TTTAAGTACG
    CCTGTATCAG TCAGCATGAC CACCATGACA TCCACCCCCT ATAGCGCCAT TAGTGGACAA
    ACACAGCCGC CTCCGACGCA ATTCCCTATC GTTCCTCTAC CCCAGCAGAA CGTGCATCCA
    CAACCCGGCC CGCACCGTCA GCCCCTGCAG TACGGCCCTT CACCCCTGCA GCAGGCCCAG
    CTCCCGGTGC CTGCAGCAGG ACAAACACCG CCCCCAACCA ATCCAGACGC TGTATTCTGC
    TCATGCTCTT CCTCCTCCTG CTCCTCATCC TCCTGTTCCA CCTCCAGTGC CAAACTTCAC
    CCCAAACCCT CCACGTCCAC CCTTCCTTTA GGACAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325645.1
    CDS1064..6925
    Translation

    Target ORF information:

    RefSeq Version XM_021469970.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-151g10.3 (si:dkey-151g10.3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCGACAG ACCCAGGAGA GCCCACTGCC ACGGAGGAGT CCTCTGGAGA GAAACCTGAT 
    GGAGAGAGGG AGGAGGAGAG TGACCAGGGG AACAGGGCCG CCCGTGAGAG AGAGAGGGTG
    CACAGCACTC CATCTGACTT ACCCTCCTCC CAGAGCCAGG AAAGAAGAGC ATGGGGAGCA
    GGAGGAGGAG GAGGCGGAGT GGAGGACTGT AAAGAAACGG AGGCAACGGT CAGACCTCTG
    ACCTTCGTCA TGCCCTCCTC CCCAGCGACC CACGAACCTT GCCGGAAACG GGAGAACAAA
    CGCTTTTTTC GGAAGAGTGT AGAGATCTGC GAAGAAGATG ATGAGGTGGA TGATATCCCA
    GACGCCCCGC ATAGCGCCCC TCACCTCGAG TTTTGTGCCT CCGACTCCGT GTTCACCAGC
    GGAGCCCAGC AGTTACCCAC CAGTGCCTCG GCTGCCTTGG GTGAAGATGG CACTCAGGGC
    ACACAGGATC CTGATAAGGC TGGCATTTCT GTGCCCACCC TGAAAGGGAA GGAGAGAGAT
    AGAGAACAGG AAGAAGAGGC TGAGATGAAG GCCGTTGCCA CTTCGCCTGG TGGACGCTTT
    CTAAAATTTG ACATCGAGCT TGGCAGAGGA GCATTTAAAA CTGTTTACAA AGGCCTGGAC
    ACGGAAACCT GGGTGGAAGT TGCCTGGTGT GAGCTGCAGG ATCGAAAGCT GACCAAGGCG
    GAGCAGCAGC GATTTAAAGA GGAGGCTGAA ATGCTGAAGG GTCTGCAGCA TCCCAACATT
    GTGCGTTTCT ATGACTCTTG GGAGTCTGTT CTTAGAGGGA AGAAGTGCAT CGTCCTTGTG
    ACCGAGCTCA TGACCTCAGG AACACTCAAA ACGTATCTTA AGCGCTTTAA GGTGATGAAG
    CCCAAGGTTT TGCGCAGCTG GTGCCGGCAG ATTCTGAAGG GTCTCCAGTT CCTACACACT
    CGTACGCCAC CCATTGTTCA CCGTGACCTC AAGTGTGATA ACATCTTCAT CACAGGCCCA
    ACAGGATCAG TAAAAATTGG GGACCTGGGT CTTGCCACCC TCATGCGGAC ATCGTTTGCC
    AAGAGTGTCA TAGGAACTCC AGAGTTCATG GCTCCAGAGA TGTATGAGGA GCACTATGAC
    GAGTCAGTGG ATGTCTATGC CTTTGGCATG TGCATGCTAG AAATGGCTAC CTCAGAGTAC
    CCCTATTCAG AATGCCAAAA TGCTGCACAG ATCTACCGCA AAGTCACAAG TGGCATCAAG
    CCTGCCAGTT TTGACAAAGT AAATGACCCT GAGGTAAAAG AAATCATTGA GGGCTGCATT
    CGCCAAAACC GATTAGAGCG GCTCTCAGTG AAGGACCTCC TAAATCATGC CTTCTTCGCA
    GAGGACACCG GTGTTCGTGT GGAGCTTGCA GAGGAAGATA CAGGCTGCAA AGACTGCCTG
    GCCCTGCGCA TCTGGGTTGA AGAGCCAAAG AAACTCAAAG GCAAGCACAA GGACAATGAG
    GCCATCGAGT TCAGCTATGA CCTGGAGAAC GATAGTGCTG AAGAAGTTGC TCTAGAGATG
    GTAAAATCTG GCTTTTTCCA TGAAAGCGAC GCTAAGGTAG TGGGGAAGTC CATCAGAGAC
    AGGGTGACTC TAATTAAGAA GTCTCGTGAA CGGCGGCTCC TCCAACAGCA GCAGCAGCAG
    CAGCAGCAAC AACAGCAAAG TCTGGATGAA CGCCGTGATT CTTCTGTCCT TACCACCTCC
    ATTCCTTATG TTCATCCTTC TTGCACCACC TCCTCACAGG GCACAGGTGC CGCTGCAGCT
    GCCGGGGGTC AAGGAGAGGC AGAGGAGCTG CCAGAAGTTG ACCAACATGT CCAACAACAA
    GGCACTGCAT CAGGCCTCAC AGAGAGTGAG AGTCTTGCTG CTGTCCCTGG ACAGAGCCAA
    GCCTTTTCAG CTCCCGATTC AGGGAATCCT TGTGCTCATG CTCAAACCAG CTACCCCACT
    GGCACATCTG GAGCGTCTCA CCCTCAACTG CTCCCAATTG GTCAGAGCGG AGGGGTTCCA
    AATGTGCCGG TTGGCCAGAA TGGTGGGATA TCTGGCATTT CCATTGGCCA AAGTGGTGGT
    GGGGCTCAGA CTTTCATTCA ACCAGTCACT ATGGCTTCAC AGGTCCCATT AATTGTGCCT
    CAACAATATT CTCAGGAAAG CCTAATTCAC TCTGCCAACA GGGGATCTTT ATTATGCTCT
    GCTCCTACCA ATGGAGATGA GGGCCAGTTG GTGTGGCCTA GTGGAAAACT AGATAAGGCA
    AAATGTCAGA GGAGATCATC CTGTCAGAAG ACTGAAAAAA CTCACTTCCA ACTCTGCATG
    TTGCAGGTCT CATGCACAGG AGACAACATG GTAGAATGTC AGTTGGAGAC TCACAGCAAC
    AAGATGGTCA CGTTCAAGTT TGACATTGAG GGAGATGCAC CTGAAGACAT TGCAGATTAC
    ATGGTGGAGG AGGACTTCGT CCTTGAGCCT GAAAAAGAAA AATTTGTTGA GGAGCTGAGA
    TGTATAGTGA AGAAAGCTCA AGCAATATTA AGTACTCAGT CGCAGACTGG ATCCCTGGAG
    CAGCTCCAAG TGAGCACCCC ATCCAGTGCA ACAATGGATT CAGCCCCTCA GTCTTCTCCA
    GTAGGTCGCT GGCGGTTCTT CATTAACCAG ACTATTCGTC ACCGTGATTC ACACTCCAAC
    CAGGGAGCTT CGACTCCCCC TCCAGTGGGG GAGAACAGAG TTCCCAAGTC CATAGAAACC
    GAGAAAGATC ATGAGAGCCC GCAGCGCTCC GAGTCTTTTG CTGGAATAGC ATCTTCCCCA
    AGTTCTTCAC TATCTGCATC CTCTCCTCCA GTCTCTACTG TATCCGCTCC TGCATCCATG
    TCAGCCTCAG CCATGGCTGC TCAAAAAGAT TTTACACCTG CTGCCTTGCC AGCAGTAGAG
    AAAGTCTCCT CTACTATGCT GACCGCAGGT CCAGTCCCTG TCATGGCCTC CAGCACTTCT
    GCCACTCCTT TTCCTGCAGC CATGGCCCTC ACAGGCTCTA ATGTGGACCA AGGAGAATCT
    GCTGTTAAAG GTGAGGGTCA ACAGCCTTAT GTGACTCCTC CAACGTCACT GTTGACCCAT
    GCTGACCCAT CTCAGCACTT AATTGTTCAA ACCCCAATGG CACCCTCACA GATGCCAGAT
    ATACCTCAAC AAAGCCAGAC ACTTCAATCA ACATTGATCA CAACTGAACA AGTTCAGCAA
    CCGCAGATTA CCCCTCAGCC ACTTCTGCAG GATGCTCCAC AGCTACCTAA ACCAGAATCA
    AAATCGCTAC CACAGGCAGT GCCTAAAGAG ATGGTTCCTT TGCATGAAGG TCTTTCAGCT
    GCCCAAGTTC AGCAGGTCCC TCTCCAGCAA GTACCAGTAG AATTGCAACA GCAGCCAATG
    CAGCAGACCA CATTCTTACA GCAAACATCC AGTGCAGAGC AGATACAGCA GCACATGGCA
    GAACAGTTTT TATCTCAAGA AACCAAATCA GCTCCAATGA TACTGACCAT TCATCCACAG
    CATGGCTTCC AGACCCAGGA TCCTCAGCAG GTTCAAGCTC CATTGCAGTC TATGATCCCC
    CAGCAGGCTC CAATTGAGCA AACCCAGACA TTATATAAAC AGGTCCCACA TGATATGATG
    CCAGTGGCAA AAACCGCAGT GCAGAAGCAA GTGTCTTTCC AGCAGTCAGA GTCTGAGCTG
    TCTACGGGTG AGGACACCGT CAGCCACTCT GCACTTCCGC ATCCCTCTTT AGACACCTCT
    CTTCCACCCC TCCCTCTCTC TGAAACAGCT CTTCCTGCCC TCTCCCACAC ATTCACTCCT
    TCTCCAGCAC AGCCTTCTTC AGTAGCAGAG TCGGACAGTG AAGGCCCTCC CAAAATTGAG
    TTTGTGGATA ACCGAATCAA GACTTTGGAT GAGAAATTGA GGACTCTGCT CTATCAGGAG
    CACGGCGGCA GTGGAGCAGC TGTGGGCAGT GCCTCCACAG GAGGAACTGC TCCAGCCTGC
    TCCACTTCAG GATCTGCCAC AGCCTCTACT GCAGTTGATG AGTCTTCAGA AGTCCCCTAC
    GCATTCTCCC TACCTCCTGC CACCTCCTCG GACACCTCCC CTCACTCCAC CTCTTCCACA
    ACCTCCTCCA CCACCCCTCG CTCTTCCTCC ACCTCCCCGG ATGGAGAGCG AGAGAAAGCA
    GGCGAGGAGA CGACGACTAT CCCACCTGCG GTACAACAAC AGCCTGCCTC CTCTACATCC
    CCTCCATGCT CTCTTCTTTC CCATCCACAG GAGCCTGGAT CCCCCAAAGT TACTATTCAA
    TCATCAGTAA GCGCTCCCCC TTCCCTCTCT GACAGCTGCA CCCCTGGAGA TGTTCTGTTG
    CCTTCCTCTT CTCAGCCGCC CGTCCCACTG TGGCCTGGAC AGCAGCAGCA CAATGAAGGA
    GGTGGATATT TTGGCCTAAA CCTGACATGT CCTAGTATCA GAAATCCTGT TAGCAAGAAA
    TCCTGGACTC GCAAATTCAA AAGCTGGGCA TGCAAACTGC GCCACTCCGC CAGCTTGTTC
    AAGAAGCCCA GAGTCCAGCA AGATGGCAAC TCTAGCCAGG GGATGAAGGA GGCATCTACA
    GATGACCAAC CCTGCTCACG CAGAGGCCGT TTCCAGGTGA TTCCAGTATC CCAGTCTGCA
    GAGACCCTTC CAACGCAGGA AGCTCCACCA AGCCAAATGA TGAGAGTTGG CCGTTTCTCA
    GTCACTCAAG CTGAACATCG AGAGGAGCAT TTGACCGACA GCTCCCCTGT GTCACCTGAC
    CTGGAGCGAG AGAGGAGGAA AACGAAAGAC GAGAGGAGAA CGACAGCACC CAGCCACCCA
    TCCAAGAGCC ACGCGCACTC ACCCCTGGGC AGCAGTGATG ATGAGAGCGA GGTGGAGGAT
    GAAGACTTAA GGAAAGAACT GCACAAACTC AGAGAGAAAC ACATCAAGGA GGTGGTGTCC
    CTGCAGGCAC AGCAGAACAG AGAACTACAG GATTTGTACA AGCAGCTCCG CTCTCTCAAA
    GACCACAGAC AGCCAATGTC TATGAGCCTG TCCCGCACCT CTCCTCTGCC CTCCGCACCC
    CTCGCTATCT CCCCGCGCAG ACCCCGACCC GCCAAAGCCA AGCTCCGGCC TCGGCCGCAC
    TCTCACATGG ATAACAACGG AGTCGCACAC CAAGGGAGTC TTCAGCAGTC TAGCAGTTAT
    TCCGGTGGGG AGCAAAGCCG GCTACATTCA CACTGCAAGC CAGAGAAATC ACAATCCCTG
    ACATCTAACA GAGACCACAG TCCTTCAGCC CAGGGTTCTG GCAACAGAAA GAGCACATTC
    ACAGATGAGC TGCATAAACT TGTCGATGAG TGGTCAAAGG AAACTGTTTG TCCAGTTCAG
    CCCAAGCCTT CGCTGAATCA GATTAAACAG ATCCAACAAG TGCAGGAGTT GGGTGGATGG
    AGCCAGCCTG CTGAGACAAC AGCTTCGTCG GGTTGGTTTT CCGGGGCCCC TTTAAGTACG
    CCTGTATCAG TCAGCATGAC CACCATGACA TCCACCCCCT ATAGCGCCAT TAGTGGACAA
    ACACAGCCGC CTCCGACGCA ATTCCCTATC GTTCCTCTAC CCCAGCAGAA CGTGCATCCA
    CAACCCGGCC CGCACCGTCA GCCCCTGCAG TACGGCCCTT CACCCCTGCA GCAGGCCCAG
    CTCCCGGTGC CTGCAGCAGG ACAAACACCG CCCCCAACCA ATCCAGACGC TGTATTCTGC
    TCATGCTCTT CCTCCTCCTG CTCCTCATCC TCCTGTTCCA CCTCCAGTGC CAAACTTCAC
    CCCAAACCCT CCACGTCCAC CCTTCCTTTA GGACAGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.