sparcl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sparcl1
Entrez Gene ID	567331
Full Name	SPARC-like 1
Synonyms	sparcl
General protein information	Preferred Names SPARC-like 1 Names SPARC-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475590.1	XP_021331265.1	SPARC-like protein 1 isoform X1
NM_001130605.1	NP_001124077.1	SPARC-like protein 1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The seahorse genome and the evolution of its specialized morphology.
Lin Q, Fan S, Zhang Y, Xu M, Zhang H, Yang Y, Lee AP, Woltering JM, Ravi V, Gunter HM, Luo W, Gao Z, Lim ZW, Qin G, Schneider RF, Wang X, Xiong P, Li G, Wang K, Min J, Zhang C, Qiu Y, Bai J, He W, Bian C, Zhang X, Shan D, Qu H, Sun Y, Gao Q, Huang L, Shi Q, Meyer A, Venkatesh B
Nature540(7633)395-399(2016 12)

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons.
Braasch I, Gehrke AR, Smith JJ, Kawasaki K, Manousaki T, Pasquier J, Amores A, Desvignes T, Batzel P, Catchen J, Berlin AM, Campbell MS, Barrell D, Martin KJ, Mulley JF, Ravi V, Lee AP, Nakamura T, Chalopin D, Fan S, Wcisel D, Ca?estro C, Sydes J, Beaudry FE, Sun Y, Hertel J, Beam MJ, Fasold M, Ishiyama M, Johnson J, Kehr S, Lara M, Letaw JH, Litman GW, Litman RT, Mikami M, Ota T, Saha NR, Williams L, Stadler PF, Wang H, Taylor JS, Fontenot Q, Ferrara A, Searle SM, Aken B, Yandell M, Schneider I, Yoder JA, Volff JN, Meyer A, Amemiya CT, Venkatesh B, Holland PW, Guiguen Y, Bobe J, Shubin NH, Di Palma F, Alf?ldi J, Lindblad-Toh K, Postlethwait JH
Nature genetics48(4)427-37(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis.
Torres-N??ez E, Suarez-Bregua P, Cal L, Cal R, Cerd?-Reverter JM, Rotllant J
Gene568(2)129-39(2015 Sep)

The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues.
Kawasaki K
Development genes and evolution219(3)147-57(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sparcl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sparcl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34939	NM_001130605.1 Latest version!	Danio rerio SPARC-like 1 (sparcl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa34939	XM_021475590.1 Latest version!	Danio rerio SPARC-like 1 (sparcl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sparcl1 ( NM_001130605.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124077.1 Danio rerio SPARC-like 1 (sparcl1), mRNA.

Price & Availability↑

CloneID	ODa34939
Clone ID Related Accession (Same CDS sequence)	XM_021475590.1 , NM_001130605.1
Accession Version	NM_001130605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	SPARC-like protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163341.1. On Jul 30, 2008 this sequence version replaced XM_690622.3. ##Evidence-Data-START## Transcript exon combination :: BC163341.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAAAG AGGTGTTTCT CTTCTGCTTT TTGGTAACAG CACTGGCTGC ATATGTACAA 
AGTAAGCCTC ATGGAAAGAA ACAGCACATC AAGCACATTG CGACTCCAAA AGAGCAGTCA 
GAGTCAGTGA AGCTTCATGG CAGTATGGAT GGAGTCCTGC CCACGTTTCT GCCTTTTGAG 
TCTAGCACTC AGGATGAAGA CACGAGCGAG GAGACTGAGA GCCGTGTGGA GATGAACCAA 
GCGGAGGCAG AAGAGCCTGA AGGTTCAGAT GGAGGTGAAA AGAAGGAACC TTTACTCCTG 
AGTGATGAGG CATTGACTCA GCTCCTGCAA GAACCCGTGG AGGAGGATGA AGATGATCAT 
TCTGAGAGTC TGGAGAAAGA TGAAGTGATG GACAAGGTGG AGCAGTCAAC AGAAGATGGA 
GAATCCAAAA CCGAGACTGA AAACGAGGCT GAGAGCAAGG AAGAGGATTC AAAGGACACA 
GACAGCAGTA CTGAGTCAGA GATACCAATG GATTTGGACT ACACAACTGA CAGAGATGCT 
TCTCAGCCAC TACCAATCAA ACTGGATGAG GCTAAAGTTT TAACCACTAT GGAGGACACG 
GTTGAAGATG TAATCCCCAC AGCCACAGCA AGCTATGAAT CCCAGCAATC CAACAGCGAC 
ATTGCAGAGG AACAAGAGGA ACTAGAAGAC CAAGCTCAAG AGGAGTTGAA AGAACCTGAG 
AATGACATTG AAAAGGGGAA GAATGATCAG CAGGATGAGA AGAACATCCT TGAACCTGGT 
ATTTCTGAAG GCAAGATGGC TAAAAAGGAT AAGAAGAAGA ATGAAAGTAA AAGCAATGGA 
AAGGGAAAAG GCAAAAAACA GCGGAACAGT GAGCTGCTTG AGAAGATGCA GAGTGACCAG 
GCCGGTGCAA TAGAAGACAC TGAAGAACTT GCTCAGAAGA AAGACCAAGA AAAAGTGACA 
GAGGCCACTG AAAATACAGA GTCCAAACCC AGAAAGAAGA ATGGCAAATG GACTCGCTTG 
GTGGGGATGA ATCCAGTACA GATTAGGGCC ACTATGGATC TTTACCCAGA TGTTCGCCTC 
ACCCGTCACT CCAATGGGCA GGGGGTGCCA GCTGACCCCT GTGAAAATTT CCGCTGCAAA 
AGAGGGAAAA CTTGCAAACT GAACGATGAG AACAAGCCCC TCTGTGTGTG TCAGGAGCCG 
ACAGAATGTC CTCCTAATGT AAACGATTTT GAACATGTCT GCGGAACTGA CAATAAGACA 
TATGACTCAT CTTGTCACCT TTTTGCTACC AAGTGTGGTC TTGAGGGAAC CAAATTAGGT 
CACAGGCTTC ATCTGGACTA CACTGGATCA TGCAAATTTA TTGCTCCATG TGTGGAGTCA 
GAGCTGGTGC AGTTCCCACT GCGAATGCGG GACTGGCTGA AGAACGTGCT TCTGCAGTTA 
TATGAGCATG ACTCCATGTC TCCTGGCTTT CTCACAGCTA AACAGAGAAT CAGGGTTCAG 
AAGATCTATG AGAGCGAGAG ACGTCTTCAT GCAGGAGATC ACCCTGTTGA AATTCTGCAG 
CAGGACTTTG AGAAGAACTA CAACATGTAC ATCTATCCAG TGCACTGGCA GTTTGCACAG 
ATGGACCAGC ATCCTTCAGA CAGGTTTTTG ACTCACTCTG AGCTGGCTCC GCTGCGCGTT 
CCTCTAGTTC CCATGGAGCA TTGCACCTCA GTCTTCTTTC AAATGTGTGA TGCCGACAAG 
GACAAACTGG TCTCCTTCAA AGAGTGGTGC AGCTGCTTTG GCATTAAGGA AGAGGATATG 
AATACCAATC TGCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124077.1
CDS	82..1902
Translation	MKKEVFLFCFLVTALAAYVQSKPHGKKQHIKHIATPKEQSESVKLHGSMDGVLPTFLPFESSTQDEDTSEETESRVEMNQAEAEEPEGSDGGEKKEPLLLSDEALTQLLQEPVEEDEDDHSESLEKDEVMDKVEQSTEDGESKTETENEAESKEEDSKDTDSSTESEIPMDLDYTTDRDASQPLPIKLDEAKVLTTMEDTVEDVIPTATASYESQQSNSDIAEEQEELEDQAQEELKEPENDIEKGKNDQQDEKNILEPGISEGKMAKKDKKKNESKSNGKGKGKKQRNSELLEKMQSDQAGAIEDTEELAQKKDQEKVTEATENTESKPRKKNGKWTRLVGMNPVQIRATMDLYPDVRLTRHSNGQGVPADPCENFRCKRGKTCKLNDENKPLCVCQEPTECPPNVNDFEHVCGTDNKTYDSSCHLFATKCGLEGTKLGHRLHLDYTGSCKFIAPCVESELVQFPLRMRDWLKNVLLQLYEHDSMSPGFLTAKQRIRVQKIYESERRLHAGDHPVEILQQDFEKNYNMYIYPVHWQFAQMDQHPSDRFLTHSELAPLRVPLVPMEHCTSVFFQMCDADKDKLVSFKEWCSCFGIKEEDMNTNLLF

Target ORF information:

RefSeq Version	NM_001130605.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPARC-like 1 (sparcl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130605.1

ORF Insert Sequence:

ATGAAGAAAG AGGTGTTTCT CTTCTGCTTT TTGGTAACAG CACTGGCTGC ATATGTACAA 
AGTAAGCCTC ATGGAAAGAA ACAGCACATC AAGCACATTG CGACTCCAAA AGAGCAGTCA 
GAGTCAGTGA AGCTTCATGG CAGTATGGAT GGAGTCCTGC CCACGTTTCT GCCTTTTGAG 
TCTAGCACTC AGGATGAAGA CACGAGCGAG GAGACTGAGA GCCGTGTGGA GATGAACCAA 
GCGGAGGCAG AAGAGCCTGA AGGTTCAGAT GGAGGTGAAA AGAAGGAACC TTTACTCCTG 
AGTGATGAGG CATTGACTCA GCTCCTGCAA GAACCCGTGG AGGAGGATGA AGATGATCAT 
TCTGAGAGTC TGGAGAAAGA TGAAGTGATG GACAAGGTGG AGCAGTCAAC AGAAGATGGA 
GAATCCAAAA CCGAGACTGA AAACGAGGCT GAGAGCAAGG AAGAGGATTC AAAGGACACA 
GACAGCAGTA CTGAGTCAGA GATACCAATG GATTTGGACT ACACAACTGA CAGAGATGCT 
TCTCAGCCAC TACCAATCAA ACTGGATGAG GCTAAAGTTT TAACCACTAT GGAGGACACG 
GTTGAAGATG TAATCCCCAC AGCCACAGCA AGCTATGAAT CCCAGCAATC CAACAGCGAC 
ATTGCAGAGG AACAAGAGGA ACTAGAAGAC CAAGCTCAAG AGGAGTTGAA AGAACCTGAG 
AATGACATTG AAAAGGGGAA GAATGATCAG CAGGATGAGA AGAACATCCT TGAACCTGGT 
ATTTCTGAAG GCAAGATGGC TAAAAAGGAT AAGAAGAAGA ATGAAAGTAA AAGCAATGGA 
AAGGGAAAAG GCAAAAAACA GCGGAACAGT GAGCTGCTTG AGAAGATGCA GAGTGACCAG 
GCCGGTGCAA TAGAAGACAC TGAAGAACTT GCTCAGAAGA AAGACCAAGA AAAAGTGACA 
GAGGCCACTG AAAATACAGA GTCCAAACCC AGAAAGAAGA ATGGCAAATG GACTCGCTTG 
GTGGGGATGA ATCCAGTACA GATTAGGGCC ACTATGGATC TTTACCCAGA TGTTCGCCTC 
ACCCGTCACT CCAATGGGCA GGGGGTGCCA GCTGACCCCT GTGAAAATTT CCGCTGCAAA 
AGAGGGAAAA CTTGCAAACT GAACGATGAG AACAAGCCCC TCTGTGTGTG TCAGGAGCCG 
ACAGAATGTC CTCCTAATGT AAACGATTTT GAACATGTCT GCGGAACTGA CAATAAGACA 
TATGACTCAT CTTGTCACCT TTTTGCTACC AAGTGTGGTC TTGAGGGAAC CAAATTAGGT 
CACAGGCTTC ATCTGGACTA CACTGGATCA TGCAAATTTA TTGCTCCATG TGTGGAGTCA 
GAGCTGGTGC AGTTCCCACT GCGAATGCGG GACTGGCTGA AGAACGTGCT TCTGCAGTTA 
TATGAGCATG ACTCCATGTC TCCTGGCTTT CTCACAGCTA AACAGAGAAT CAGGGTTCAG 
AAGATCTATG AGAGCGAGAG ACGTCTTCAT GCAGGAGATC ACCCTGTTGA AATTCTGCAG 
CAGGACTTTG AGAAGAACTA CAACATGTAC ATCTATCCAG TGCACTGGCA GTTTGCACAG 
ATGGACCAGC ATCCTTCAGA CAGGTTTTTG ACTCACTCTG AGCTGGCTCC GCTGCGCGTT 
CCTCTAGTTC CCATGGAGCA TTGCACCTCA GTCTTCTTTC AAATGTGTGA TGCCGACAAG 
GACAAACTGG TCTCCTTCAA AGAGTGGTGC AGCTGCTTTG GCATTAAGGA AGAGGATATG 
AATACCAATC TGCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sparcl1 ( XM_021475590.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331265.1 Danio rerio SPARC-like 1 (sparcl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa34939
Clone ID Related Accession (Same CDS sequence)	XM_021475590.1 , NM_001130605.1
Accession Version	XM_021475590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	SPARC-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAG AGGTGTTTCT CTTCTGCTTT TTGGTAACAG CACTGGCTGC ATATGTACAA 
AGTAAGCCTC ATGGAAAGAA ACAGCACATC AAGCACATTG CGACTCCAAA AGAGCAGTCA 
GAGTCAGTGA AGCTTCATGG CAGTATGGAT GGAGTCCTGC CCACGTTTCT GCCTTTTGAG 
TCTAGCACTC AGGATGAAGA CACGAGCGAG GAGACTGAGA GCCGTGTGGA GATGAACCAA 
GCGGAGGCAG AAGAGCCTGA AGGTTCAGAT GGAGGTGAAA AGAAGGAACC TTTACTCCTG 
AGTGATGAGG CATTGACTCA GCTCCTGCAA GAACCCGTGG AGGAGGATGA AGATGATCAT 
TCTGAGAGTC TGGAGAAAGA TGAAGTGATG GACAAGGTGG AGCAGTCAAC AGAAGATGGA 
GAATCCAAAA CCGAGACTGA AAACGAGGCT GAGAGCAAGG AAGAGGATTC AAAGGACACA 
GACAGCAGTA CTGAGTCAGA GATACCAATG GATTTGGACT ACACAACTGA CAGAGATGCT 
TCTCAGCCAC TACCAATCAA ACTGGATGAG GCTAAAGTTT TAACCACTAT GGAGGACACG 
GTTGAAGATG TAATCCCCAC AGCCACAGCA AGCTATGAAT CCCAGCAATC CAACAGCGAC 
ATTGCAGAGG AACAAGAGGA ACTAGAAGAC CAAGCTCAAG AGGAGTTGAA AGAACCTGAG 
AATGACATTG AAAAGGGGAA GAATGATCAG CAGGATGAGA AGAACATCCT TGAACCTGGT 
ATTTCTGAAG GCAAGATGGC TAAAAAGGAT AAGAAGAAGA ATGAAAGTAA AAGCAATGGA 
AAGGGAAAAG GCAAAAAACA GCGGAACAGT GAGCTGCTTG AGAAGATGCA GAGTGACCAG 
GCCGGTGCAA TAGAAGACAC TGAAGAACTT GCTCAGAAGA AAGACCAAGA AAAAGTGACA 
GAGGCCACTG AAAATACAGA GTCCAAACCC AGAAAGAAGA ATGGCAAATG GACTCGCTTG 
GTGGGGATGA ATCCAGTACA GATTAGGGCC ACTATGGATC TTTACCCAGA TGTTCGCCTC 
ACCCGTCACT CCAATGGGCA GGGGGTGCCA GCTGACCCCT GTGAAAATTT CCGCTGCAAA 
AGAGGGAAAA CTTGCAAACT GAACGATGAG AACAAGCCCC TCTGTGTGTG TCAGGAGCCG 
ACAGAATGTC CTCCTAATGT AAACGATTTT GAACATGTCT GCGGAACTGA CAATAAGACA 
TATGACTCAT CTTGTCACCT TTTTGCTACC AAGTGTGGTC TTGAGGGAAC CAAATTAGGT 
CACAGGCTTC ATCTGGACTA CACTGGATCA TGCAAATTTA TTGCTCCATG TGTGGAGTCA 
GAGCTGGTGC AGTTCCCACT GCGAATGCGG GACTGGCTGA AGAACGTGCT TCTGCAGTTA 
TATGAGCATG ACTCCATGTC TCCTGGCTTT CTCACAGCTA AACAGAGAAT CAGGGTTCAG 
AAGATCTATG AGAGCGAGAG ACGTCTTCAT GCAGGAGATC ACCCTGTTGA AATTCTGCAG 
CAGGACTTTG AGAAGAACTA CAACATGTAC ATCTATCCAG TGCACTGGCA GTTTGCACAG 
ATGGACCAGC ATCCTTCAGA CAGGTTTTTG ACTCACTCTG AGCTGGCTCC GCTGCGCGTT 
CCTCTAGTTC CCATGGAGCA TTGCACCTCA GTCTTCTTTC AAATGTGTGA TGCCGACAAG 
GACAAACTGG TCTCCTTCAA AGAGTGGTGC AGCTGCTTTG GCATTAAGGA AGAGGATATG 
AATACCAATC TGCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331265.1
CDS	210..2030
Translation	MKKEVFLFCFLVTALAAYVQSKPHGKKQHIKHIATPKEQSESVKLHGSMDGVLPTFLPFESSTQDEDTSEETESRVEMNQAEAEEPEGSDGGEKKEPLLLSDEALTQLLQEPVEEDEDDHSESLEKDEVMDKVEQSTEDGESKTETENEAESKEEDSKDTDSSTESEIPMDLDYTTDRDASQPLPIKLDEAKVLTTMEDTVEDVIPTATASYESQQSNSDIAEEQEELEDQAQEELKEPENDIEKGKNDQQDEKNILEPGISEGKMAKKDKKKNESKSNGKGKGKKQRNSELLEKMQSDQAGAIEDTEELAQKKDQEKVTEATENTESKPRKKNGKWTRLVGMNPVQIRATMDLYPDVRLTRHSNGQGVPADPCENFRCKRGKTCKLNDENKPLCVCQEPTECPPNVNDFEHVCGTDNKTYDSSCHLFATKCGLEGTKLGHRLHLDYTGSCKFIAPCVESELVQFPLRMRDWLKNVLLQLYEHDSMSPGFLTAKQRIRVQKIYESERRLHAGDHPVEILQQDFEKNYNMYIYPVHWQFAQMDQHPSDRFLTHSELAPLRVPLVPMEHCTSVFFQMCDADKDKLVSFKEWCSCFGIKEEDMNTNLLF

Target ORF information:

RefSeq Version	XM_021475590.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPARC-like 1 (sparcl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475590.1

ORF Insert Sequence:

ATGAAGAAAG AGGTGTTTCT CTTCTGCTTT TTGGTAACAG CACTGGCTGC ATATGTACAA 
AGTAAGCCTC ATGGAAAGAA ACAGCACATC AAGCACATTG CGACTCCAAA AGAGCAGTCA 
GAGTCAGTGA AGCTTCATGG CAGTATGGAT GGAGTCCTGC CCACGTTTCT GCCTTTTGAG 
TCTAGCACTC AGGATGAAGA CACGAGCGAG GAGACTGAGA GCCGTGTGGA GATGAACCAA 
GCGGAGGCAG AAGAGCCTGA AGGTTCAGAT GGAGGTGAAA AGAAGGAACC TTTACTCCTG 
AGTGATGAGG CATTGACTCA GCTCCTGCAA GAACCCGTGG AGGAGGATGA AGATGATCAT 
TCTGAGAGTC TGGAGAAAGA TGAAGTGATG GACAAGGTGG AGCAGTCAAC AGAAGATGGA 
GAATCCAAAA CCGAGACTGA AAACGAGGCT GAGAGCAAGG AAGAGGATTC AAAGGACACA 
GACAGCAGTA CTGAGTCAGA GATACCAATG GATTTGGACT ACACAACTGA CAGAGATGCT 
TCTCAGCCAC TACCAATCAA ACTGGATGAG GCTAAAGTTT TAACCACTAT GGAGGACACG 
GTTGAAGATG TAATCCCCAC AGCCACAGCA AGCTATGAAT CCCAGCAATC CAACAGCGAC 
ATTGCAGAGG AACAAGAGGA ACTAGAAGAC CAAGCTCAAG AGGAGTTGAA AGAACCTGAG 
AATGACATTG AAAAGGGGAA GAATGATCAG CAGGATGAGA AGAACATCCT TGAACCTGGT 
ATTTCTGAAG GCAAGATGGC TAAAAAGGAT AAGAAGAAGA ATGAAAGTAA AAGCAATGGA 
AAGGGAAAAG GCAAAAAACA GCGGAACAGT GAGCTGCTTG AGAAGATGCA GAGTGACCAG 
GCCGGTGCAA TAGAAGACAC TGAAGAACTT GCTCAGAAGA AAGACCAAGA AAAAGTGACA 
GAGGCCACTG AAAATACAGA GTCCAAACCC AGAAAGAAGA ATGGCAAATG GACTCGCTTG 
GTGGGGATGA ATCCAGTACA GATTAGGGCC ACTATGGATC TTTACCCAGA TGTTCGCCTC 
ACCCGTCACT CCAATGGGCA GGGGGTGCCA GCTGACCCCT GTGAAAATTT CCGCTGCAAA 
AGAGGGAAAA CTTGCAAACT GAACGATGAG AACAAGCCCC TCTGTGTGTG TCAGGAGCCG 
ACAGAATGTC CTCCTAATGT AAACGATTTT GAACATGTCT GCGGAACTGA CAATAAGACA 
TATGACTCAT CTTGTCACCT TTTTGCTACC AAGTGTGGTC TTGAGGGAAC CAAATTAGGT 
CACAGGCTTC ATCTGGACTA CACTGGATCA TGCAAATTTA TTGCTCCATG TGTGGAGTCA 
GAGCTGGTGC AGTTCCCACT GCGAATGCGG GACTGGCTGA AGAACGTGCT TCTGCAGTTA 
TATGAGCATG ACTCCATGTC TCCTGGCTTT CTCACAGCTA AACAGAGAAT CAGGGTTCAG 
AAGATCTATG AGAGCGAGAG ACGTCTTCAT GCAGGAGATC ACCCTGTTGA AATTCTGCAG 
CAGGACTTTG AGAAGAACTA CAACATGTAC ATCTATCCAG TGCACTGGCA GTTTGCACAG 
ATGGACCAGC ATCCTTCAGA CAGGTTTTTG ACTCACTCTG AGCTGGCTCC GCTGCGCGTT 
CCTCTAGTTC CCATGGAGCA TTGCACCTCA GTCTTCTTTC AAATGTGTGA TGCCGACAAG 
GACAAACTGG TCTCCTTCAA AGAGTGGTGC AGCTGCTTTG GCATTAAGGA AGAGGATATG 
AATACCAATC TGCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The seahorse genome and the evolution of its specialized morphology.
Nature540(7633)395-399(2016 12)
Lin Q,Fan S,Zhang Y,Xu M,Zhang H,Yang Y,Lee AP,Woltering JM,Ravi V,Gunter HM,Luo W,Gao Z,Lim ZW,Qin G,Schneider RF,Wang X,Xiong P,Li G,Wang K,Min J,Zhang C,Qiu Y,Bai J,He W,Bian C,Zhang X,Shan D,Qu H,Sun Y,Gao Q,Huang L,Shi Q,Meyer A,Venkatesh B

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons.
Nature genetics48(4)427-37(2016 Apr)
Braasch I,Gehrke AR,Smith JJ,Kawasaki K,Manousaki T,Pasquier J,Amores A,Desvignes T,Batzel P,Catchen J,Berlin AM,Campbell MS,Barrell D,Martin KJ,Mulley JF,Ravi V,Lee AP,Nakamura T,Chalopin D,Fan S,Wcisel D,Ca?estro C,Sydes J,Beaudry FE,Sun Y,Hertel J,Beam MJ,Fasold M,Ishiyama M,Johnson J,Kehr S,Lara M,Letaw JH,Litman GW,Litman RT,Mikami M,Ota T,Saha NR,Williams L,Stadler PF,Wang H,Taylor JS,Fontenot Q,Ferrara A,Searle SM,Aken B,Yandell M,Schneider I,Yoder JA,Volff JN,Meyer A,Amemiya CT,Venkatesh B,Holland PW,Guiguen Y,Bobe J,Shubin NH,Di Palma F,Alf?ldi J,Lindblad-Toh K,Postlethwait JH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis.
Gene568(2)129-39(2015 Sep)
Torres-N??ez E,Suarez-Bregua P,Cal L,Cal R,Cerd?-Reverter JM,Rotllant J

The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues.
Development genes and evolution219(3)147-57(2009 Mar)
Kawasaki K

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sparcl1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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