nhsl1a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005158820.4	XP_005158877.2	NHS-like protein 1 isoform X2
XM_009293150.3	XP_009291425.2	NHS-like protein 1 isoform X4
XM_689979.9	XP_695071.7	NHS-like protein 1 isoform X7
XM_005158820.3	XP_005158877.1	NHS-like protein 1 isoform X2
XM_009293150.2	XP_009291425.1	NHS-like protein 1 isoform X4
XM_005158824.3	XP_005158881.1	NHS-like protein 1 isoform X8
XM_005158819.4	XP_005158876.2	NHS-like protein 1 isoform X1
XM_005158821.4	XP_005158878.2	NHS-like protein 1 isoform X3
XM_005158819.3	XP_005158876.1	NHS-like protein 1 isoform X1
XM_005158822.3	XP_005158879.1	NHS-like protein 1 isoform X5
XM_689979.8	XP_695071.6	NHS-like protein 1 isoform X7
XM_021467438.1	XP_021323113.1	NHS-like protein 1 isoform X4
XM_021467436.1	XP_021323111.1	NHS-like protein 1 isoform X2
XM_021467435.1	XP_021323110.1	NHS-like protein 1 isoform X1
XM_005158821.3	XP_005158878.1	NHS-like protein 1 isoform X3
XM_005158823.3	XP_005158880.1	NHS-like protein 1 isoform X6
XM_005158823.4	XP_005158880.2	NHS-like protein 1 isoform X6
XM_005158824.4	XP_005158881.2	NHS-like protein 1 isoform X8
XM_021467439.1	XP_021323114.1	NHS-like protein 1 isoform X5
XM_021467440.1	XP_021323115.1	NHS-like protein 1 isoform X6
XM_021467437.1	XP_021323112.1	NHS-like protein 1 isoform X3
XM_021467441.1	XP_021323116.1	NHS-like protein 1 isoform X7
XM_021467442.1	XP_021323117.1	NHS-like protein 1 isoform X8
XM_005158822.4	XP_005158879.2	NHS-like protein 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145951	XM_005158824.4 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa145944	XM_005158819.4 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145945	XM_005158820.4 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145946	XM_005158821.4 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145947	XM_009293150.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145948	XM_005158822.4 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145949	XM_005158823.4 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145950	XM_689979.9 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa22649	XM_021467435.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22649	XM_005158819.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22650	XM_021467436.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22650	XM_005158820.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22651	XM_021467437.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22651	XM_005158821.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22652	XM_005158822.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22652	XM_021467439.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22653	XM_005158823.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22653	XM_021467440.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22654	XM_689979.8 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22654	XM_021467441.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22655	XM_005158824.3 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa22655	XM_021467442.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44940	XM_021467438.1 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44940	XM_009293150.2 Latest version!	Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

nhsl1a ( XM_005158824.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158881.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa145951
Clone ID Related Accession (Same CDS sequence)	XM_005158824.4
Accession Version	XM_005158824.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158824.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGCGG TTTAAAGTCC TAGACTGCCT ATCACAGTCT 
TGCCTCTCTC TCTGCTGTCA TTTGAGATCT TGGAAAACAA AGGCTTCCGA TTCACCTAAT 
GAAGAGGATA AACTTGTGTA CTCAATGCCG CCACAAACCC CTCTGCTGGA GCATGGCTCT 
GACATGGACA TCCAGAGAAG CTGGACCAGC TGTCTACCTT TGCCCACTCC CGAGGAGAAG 
ATGAGACTGC AGGCCCAATC GGTGGCTACT GATATAATTC CTATCAACAT TACAGGTGAG 
AACTTTGACC GCCAGGCCAG CTTTCGCCGC TCCATGGCAA ACACTGATAC TATTATACGA 
AGACCTAAGA GAGTTCAAAG AAGAAAGACA ATAACAGGAG TGCCTGACAA CATCCAGACT 
GAACTAGCTA ATAAAGAACA AGCTGACTCC AGAATACATT CGAGTATGGC AGATCCGTAC 
TCCACCCTCA GTCGAGTGGG AAGTGTAAAC TCCACCCTGA AACGCTCAGA CACCCGTGAT 
TCCTGTTGTC AAACAGAAGA GGTGAAGATT GTTCCTCCCT CAGTACGCAG AATCAGAGCA 
CAAAGAGGCC ATGGAATAGC TGCCCAAATG GCCAGTGTCT CCTCCTCAAG CAGCATTTCC 
ACCATAAGTG ACTGTAATGG CATTAGCTAC ACACAGCTTA ATGACAGTGA TCAGAGCTTT 
CAAAGTTTAC CAAGACAAGG TTCTCGCATC CCGCAACAAC ACAGTGAAAC AAAGTACAAT 
AGCTCCCCCT ACAGGGCGAA TTCTGGTTCT ACAAGCCCCT TGTCCTACCA TTTTAACATG 
CAGCATGACT CTTCATCATC GCTGCATATG CTGTCTGACT CGAGAAGCAA GCTTACTGAA 
AATCTAAATT ACGAAGGCTC TGATAAAAAT AATACCTCAA GCTTGTTTAG TCTCAGTGGT 
GCTCTACAGA ACTCCATTCC TTATGGATAC AAAAGGACAG AGGACAACCA AATGCAATCA 
CCTCATTCGT CAGTCCAGTT GCTCAATTCT GAGGATGCCT CATTGTCGGG TGTCTCCTCG 
TGTTATGAAT CCACAGCGTC CCTCTGTACA GGTATTCATA CAGAGACTGA GTCCCAGTGC 
AGCACACTTG ATGGCAGAAA CTGCTGCAGT CCTAACTGTG TCCGCAGGGA TTCCAACTTC 
TCAGAGAGCA GCAACCAAAG CTGCAGCACA CTAACCACGG ACCAATGGAC GTATGAAGTT 
TCTCCAAAAG ACAATGTTAC TTCAAGTTGC TCCTCACCTG TCAGCCATAT ATACCACGCA 
GAGGAACATT CTCCCAGCAA GACAGATACA AGCTCATTGT TCTCTGTTGA CAATGAAGGT 
TACTACACCT CCATGCGCCT AGACTCTGGT TTGAAGTCAC ACAGTCACGG CTGCATCAAT 
AAAGCAGGAG AACAAAAGCA CAGTTTGTAT GAGTGCAAAG ACCACCACAG TCAAAGTGAT 
ACTGCAAGTC TGCATAGCAA CCGCTCCCTG ACTCGTAGCA TCTCTCTGCG AAAAGCCAGG 
AAGCCCCCAC TCCCTCCAGA AAGAACCGAC TCTTTAAGGC GTAAACCCCA GAGGAAGAGT 
CGCCACAGTG GTTCAGTCCT TAATGAGCAG TTAATCTCTA GTCTCCAGCA GTCCTTACAA 
CTTAACCTAA ATACTCAAAC TACATCTTGC TCTGAACAGT CACCCTGTGG TAGGTTTGAA 
GACCCTTGGG TCCTCCGACC GAGAAGTCAG AGTATCATAA GTGCAGCTAG CAGTGGGGTA 
TCTGCACCAG CTGCTGTATG TCCGGTCACA CCAACACACA GTGACAGCAG CAGTCAGCGC 
TCAGACTATG CAGAGTCATG GGACTTCTAC ATGGATTGCC CATGTCCACG GTCTGACCAG 
AGTCTTTCCT CACAAATCAC AAGGTCTGCA AGTGCTGAGG AGAATCATGG AGTCATAGGT 
AATGCATCCT ACAACAGTAA ATTCCCATCA TCCCCCTTCA GCAATAGTCA GTCAAAATTG 
ACCTCATCTC CTGACAAAGT CCATCGACTC ACGTCACCAT CAAGTGGCTA CTCAAGTCAG 
TCTAACACTC CAACTGCAGG CACTCCAGTT ACGTCTCTAA TAAGAGCAAA GTCTCCTGCG 
GGGAGACCGA AACCAAAAGT GCCAGAAAGA AAGTCCTCTC TACGATCTTC TATATCATCC 
TCTTCTACAT CCCTGTCCTC CAACACCTCA GATTCCATCA GGAACATTCC TCCACCTCCA 
CCTTTACCTG ACATGACAAC TGCATTATGT GGGTCGCCCA GTTCTGCTTC TTTCTCTCCA 
ACAGCGGCTA AAATAGACCC AATACCCTCT TTTACATCTC TACAGGATGT ATCATCTGCC 
TTGGAAGATG GGCAAAATAC ACTTTCAGAT ACATCTGCAA CACCTGAGGA ATGTGATAGT 
CCTGATTTTC CCCCTCCCCA TTCAAATGTT TTTGAATCTC TGTCAGGCAA TGTAGATACC 
AACAGCCTCC CACCTCCTCC ACTTCTGCTT TGCAAACCTT CACTGTGCAT TTCATCTCTT 
CTTCCCCCTC CTCCTCCACC TCCTCCACTA TTACTTGAGA CCAGTCTTCA ACCAGCCACC 
ATTTTGAAGA CAGAGAGCAG TCTTACACCT ACAACAGCTG TAAAATGTGA AAAAGAAGTA 
AACATCAGTC ACACTGGAGA GCAAGAAAAG ACTCAGAGAC CAATAATCAC TGCCCAGGCC 
CTTCAGATGG TGCAGTTGCG ACCTGTTAAG CTCAAGCAAA TAGAAACTAT TTTTACATCT 
GTCAGTTTTG ATCATTCTGA TCACCAAGCA CAAAACCATT CTGATACAAA CTCAGGAAAG 
TCCCTGGAAA TTGATAATAC AGGGCAAACA GTGACCGTCA ATTGTGTTAC TCAGTCAGAA 
AATAGTATGG AAACATCTGA ATGTGAATAT TCGGTGAACA GATTCATAGA CGATCTCCCT 
GAGGTATCAA CTAGCACTCT ACCCCCTGAC CTAGATGAAC AATTAAAAAA AACTGAGCAA 
AATAATCCAG TGCACCTCTC TTCCAAGTCC ACAAATTCCT TAGCTTCTCC TCTGAGTTCT 
CCAATGAAAC AGAAGCCTCC TATATCACCC AAAAAACCCA ATCTTTCCCT TATTATACCA 
CCCATAATGC ACTTGCCATT TTCCAACGGA CTCAAGCTTC AGCAGGTTCA TGAAGCACAA 
GAGACACTAG AGTCAGAGCA AGTGAATGGT GCATCCTCGA CAAACACAGT TAATTTGCAA 
GCAATTCCTT TAGACCTTGA GGTAGAGGAA GAGAGTGACT CAGACTTATC AACCCCTGTG 
GTCAAGAGCA GACTATCTTT CTGCAGCGAG TTATCATCAA GCAGTTTCCA AGACCTGCAA 
CTTTCTGATT ACATTCTTAA TGAGCATGAC CTCGGCCTGA GCGATAAAGA CTTTGGGCTC 
TGTGATGATA AAAGCACATC AGATGATGGT TCAAGCAGCA GCTCAGGATC CATCAGCTTT 
AAAGAGGATG AACATGAGGA AAACGGTGCT GTGTTTGACT CAATCACTGA AAGCACATCT 
GACATTAACG CTAGCGGAGA AGCTGTGGAG GGGATGGTGA CGCCTGTTCG CCCTCGTACT 
ACAGAGGACC TCTTTGCTGC AATTCATAGT CTACGTAGAC AGAACATGAA CATCCAATTA 
CAGGTCAAAA AGGAAGGTCC TGGGCCGTGG CGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158881.2
CDS	280..4578
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHSLRRQNMNIQLQVKKEGPGPWRF

Target ORF information:

RefSeq Version	XM_005158824.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158824.4

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGCGG TTTAAAGTCC TAGACTGCCT ATCACAGTCT 
TGCCTCTCTC TCTGCTGTCA TTTGAGATCT TGGAAAACAA AGGCTTCCGA TTCACCTAAT 
GAAGAGGATA AACTTGTGTA CTCAATGCCG CCACAAACCC CTCTGCTGGA GCATGGCTCT 
GACATGGACA TCCAGAGAAG CTGGACCAGC TGTCTACCTT TGCCCACTCC CGAGGAGAAG 
ATGAGACTGC AGGCCCAATC GGTGGCTACT GATATAATTC CTATCAACAT TACAGGTGAG 
AACTTTGACC GCCAGGCCAG CTTTCGCCGC TCCATGGCAA ACACTGATAC TATTATACGA 
AGACCTAAGA GAGTTCAAAG AAGAAAGACA ATAACAGGAG TGCCTGACAA CATCCAGACT 
GAACTAGCTA ATAAAGAACA AGCTGACTCC AGAATACATT CGAGTATGGC AGATCCGTAC 
TCCACCCTCA GTCGAGTGGG AAGTGTAAAC TCCACCCTGA AACGCTCAGA CACCCGTGAT 
TCCTGTTGTC AAACAGAAGA GGTGAAGATT GTTCCTCCCT CAGTACGCAG AATCAGAGCA 
CAAAGAGGCC ATGGAATAGC TGCCCAAATG GCCAGTGTCT CCTCCTCAAG CAGCATTTCC 
ACCATAAGTG ACTGTAATGG CATTAGCTAC ACACAGCTTA ATGACAGTGA TCAGAGCTTT 
CAAAGTTTAC CAAGACAAGG TTCTCGCATC CCGCAACAAC ACAGTGAAAC AAAGTACAAT 
AGCTCCCCCT ACAGGGCGAA TTCTGGTTCT ACAAGCCCCT TGTCCTACCA TTTTAACATG 
CAGCATGACT CTTCATCATC GCTGCATATG CTGTCTGACT CGAGAAGCAA GCTTACTGAA 
AATCTAAATT ACGAAGGCTC TGATAAAAAT AATACCTCAA GCTTGTTTAG TCTCAGTGGT 
GCTCTACAGA ACTCCATTCC TTATGGATAC AAAAGGACAG AGGACAACCA AATGCAATCA 
CCTCATTCGT CAGTCCAGTT GCTCAATTCT GAGGATGCCT CATTGTCGGG TGTCTCCTCG 
TGTTATGAAT CCACAGCGTC CCTCTGTACA GGTATTCATA CAGAGACTGA GTCCCAGTGC 
AGCACACTTG ATGGCAGAAA CTGCTGCAGT CCTAACTGTG TCCGCAGGGA TTCCAACTTC 
TCAGAGAGCA GCAACCAAAG CTGCAGCACA CTAACCACGG ACCAATGGAC GTATGAAGTT 
TCTCCAAAAG ACAATGTTAC TTCAAGTTGC TCCTCACCTG TCAGCCATAT ATACCACGCA 
GAGGAACATT CTCCCAGCAA GACAGATACA AGCTCATTGT TCTCTGTTGA CAATGAAGGT 
TACTACACCT CCATGCGCCT AGACTCTGGT TTGAAGTCAC ACAGTCACGG CTGCATCAAT 
AAAGCAGGAG AACAAAAGCA CAGTTTGTAT GAGTGCAAAG ACCACCACAG TCAAAGTGAT 
ACTGCAAGTC TGCATAGCAA CCGCTCCCTG ACTCGTAGCA TCTCTCTGCG AAAAGCCAGG 
AAGCCCCCAC TCCCTCCAGA AAGAACCGAC TCTTTAAGGC GTAAACCCCA GAGGAAGAGT 
CGCCACAGTG GTTCAGTCCT TAATGAGCAG TTAATCTCTA GTCTCCAGCA GTCCTTACAA 
CTTAACCTAA ATACTCAAAC TACATCTTGC TCTGAACAGT CACCCTGTGG TAGGTTTGAA 
GACCCTTGGG TCCTCCGACC GAGAAGTCAG AGTATCATAA GTGCAGCTAG CAGTGGGGTA 
TCTGCACCAG CTGCTGTATG TCCGGTCACA CCAACACACA GTGACAGCAG CAGTCAGCGC 
TCAGACTATG CAGAGTCATG GGACTTCTAC ATGGATTGCC CATGTCCACG GTCTGACCAG 
AGTCTTTCCT CACAAATCAC AAGGTCTGCA AGTGCTGAGG AGAATCATGG AGTCATAGGT 
AATGCATCCT ACAACAGTAA ATTCCCATCA TCCCCCTTCA GCAATAGTCA GTCAAAATTG 
ACCTCATCTC CTGACAAAGT CCATCGACTC ACGTCACCAT CAAGTGGCTA CTCAAGTCAG 
TCTAACACTC CAACTGCAGG CACTCCAGTT ACGTCTCTAA TAAGAGCAAA GTCTCCTGCG 
GGGAGACCGA AACCAAAAGT GCCAGAAAGA AAGTCCTCTC TACGATCTTC TATATCATCC 
TCTTCTACAT CCCTGTCCTC CAACACCTCA GATTCCATCA GGAACATTCC TCCACCTCCA 
CCTTTACCTG ACATGACAAC TGCATTATGT GGGTCGCCCA GTTCTGCTTC TTTCTCTCCA 
ACAGCGGCTA AAATAGACCC AATACCCTCT TTTACATCTC TACAGGATGT ATCATCTGCC 
TTGGAAGATG GGCAAAATAC ACTTTCAGAT ACATCTGCAA CACCTGAGGA ATGTGATAGT 
CCTGATTTTC CCCCTCCCCA TTCAAATGTT TTTGAATCTC TGTCAGGCAA TGTAGATACC 
AACAGCCTCC CACCTCCTCC ACTTCTGCTT TGCAAACCTT CACTGTGCAT TTCATCTCTT 
CTTCCCCCTC CTCCTCCACC TCCTCCACTA TTACTTGAGA CCAGTCTTCA ACCAGCCACC 
ATTTTGAAGA CAGAGAGCAG TCTTACACCT ACAACAGCTG TAAAATGTGA AAAAGAAGTA 
AACATCAGTC ACACTGGAGA GCAAGAAAAG ACTCAGAGAC CAATAATCAC TGCCCAGGCC 
CTTCAGATGG TGCAGTTGCG ACCTGTTAAG CTCAAGCAAA TAGAAACTAT TTTTACATCT 
GTCAGTTTTG ATCATTCTGA TCACCAAGCA CAAAACCATT CTGATACAAA CTCAGGAAAG 
TCCCTGGAAA TTGATAATAC AGGGCAAACA GTGACCGTCA ATTGTGTTAC TCAGTCAGAA 
AATAGTATGG AAACATCTGA ATGTGAATAT TCGGTGAACA GATTCATAGA CGATCTCCCT 
GAGGTATCAA CTAGCACTCT ACCCCCTGAC CTAGATGAAC AATTAAAAAA AACTGAGCAA 
AATAATCCAG TGCACCTCTC TTCCAAGTCC ACAAATTCCT TAGCTTCTCC TCTGAGTTCT 
CCAATGAAAC AGAAGCCTCC TATATCACCC AAAAAACCCA ATCTTTCCCT TATTATACCA 
CCCATAATGC ACTTGCCATT TTCCAACGGA CTCAAGCTTC AGCAGGTTCA TGAAGCACAA 
GAGACACTAG AGTCAGAGCA AGTGAATGGT GCATCCTCGA CAAACACAGT TAATTTGCAA 
GCAATTCCTT TAGACCTTGA GGTAGAGGAA GAGAGTGACT CAGACTTATC AACCCCTGTG 
GTCAAGAGCA GACTATCTTT CTGCAGCGAG TTATCATCAA GCAGTTTCCA AGACCTGCAA 
CTTTCTGATT ACATTCTTAA TGAGCATGAC CTCGGCCTGA GCGATAAAGA CTTTGGGCTC 
TGTGATGATA AAAGCACATC AGATGATGGT TCAAGCAGCA GCTCAGGATC CATCAGCTTT 
AAAGAGGATG AACATGAGGA AAACGGTGCT GTGTTTGACT CAATCACTGA AAGCACATCT 
GACATTAACG CTAGCGGAGA AGCTGTGGAG GGGATGGTGA CGCCTGTTCG CCCTCGTACT 
ACAGAGGACC TCTTTGCTGC AATTCATAGT CTACGTAGAC AGAACATGAA CATCCAATTA 
CAGGTCAAAA AGGAAGGTCC TGGGCCGTGG CGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158819.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158876.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa145944
Clone ID Related Accession (Same CDS sequence)	XM_005158819.4
Accession Version	XM_005158819.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGCGG TTTAAAGTCC TAGACTGCCT ATCACAGTCT 
TGCCTCTCTC TCTGCTGTCA TTTGAGATCT TGGAAAACAA AGGCTTCCGA TTCACCTAAT 
GAAGAGGATA AACTTGTGTA CTCAATGCCG CCACAAACCC CTCTGCTGGA GCATGGCTCT 
GACATGGACA TCCAGAGAAG CTGGACCAGC TGTCTACCTT TGCCCACTCC CGAGGAGAAG 
ATGAGACTGC AGGCCCAATC GGTGGCTACT GATATAATTC CTATCAACAT TACAGGTGAG 
AACTTTGACC GCCAGGCCAG CTTTCGCCGC TCCATGGCAA ACACTGATAC TATTATACGA 
AGACCTAAGA GAGTTCAAAG AAGAAAGACA ATAACAGGAG TGCCTGACAA CATCCAGACT 
GAACTAGCTA ATAAAGAACA AGCTGACTCC AGAATACATT CGAGTATGGC AGATCCGTAC 
TCCACCCTCA GTCGAGTGGG AAGTGTAAAC TCCACCCTGA AACGCTCAGA CACCCGTGAT 
TCCTGTTGTC AAACAGAAGA GGTGAAGATT GTTCCTCCCT CAGTACGCAG AATCAGAGCA 
CAAAGAGGCC ATGGAATAGC TGCCCAAATG GCCAGTGTCT CCTCCTCAAG CAGCATTTCC 
ACCATAAGTG ACTGTAATGG CATTAGCTAC ACACAGCTTA ATGACAGTGA TCAGAGCTTT 
CAAAGTTTAC CAAGACAAGG TTCTCGCATC CCGCAACAAC ACAGTGAAAC AAAGTACAAT 
AGCTCCCCCT ACAGGGCGAA TTCTGGTTCT ACAAGCCCCT TGTCCTACCA TTTTAACATG 
CAGCATGACT CTTCATCATC GCTGCATATG CTGTCTGACT CGAGAAGCAA GCTTACTGAA 
AATCTAAATT ACGAAGGCTC TGATAAAAAT AATACCTCAA GCTTGTTTAG TCTCAGTGGT 
GCTCTACAGA ACTCCATTCC TTATGGATAC AAAAGGACAG AGGACAACCA AATGCAATCA 
CCTCATTCGT CAGTCCAGTT GCTCAATTCT GAGGATGCCT CATTGTCGGG TGTCTCCTCG 
TGTTATGAAT CCACAGCGTC CCTCTGTACA GGTATTCATA CAGAGACTGA GTCCCAGTGC 
AGCACACTTG ATGGCAGAAA CTGCTGCAGT CCTAACTGTG TCCGCAGGGA TTCCAACTTC 
TCAGAGAGCA GCAACCAAAG CTGCAGCACA CTAACCACGG ACCAATGGAC GTATGAAGTT 
TCTCCAAAAG ACAATGTTAC TTCAAGTTGC TCCTCACCTG TCAGCCATAT ATACCACGCA 
GAGGAACATT CTCCCAGCAA GACAGATACA AGCTCATTGT TCTCTGTTGA CAATGAAGGT 
TACTACACCT CCATGCGCCT AGACTCTGGT TTGAAGTCAC ACAGTCACGG CTGCATCAAT 
AAAGCAGGAG AACAAAAGCA CAGTTTGTAT GAGTGCAAAG ACCACCACAG TCAAAGTGAT 
ACTGCAAGTC TGCATAGCAA CCGCTCCCTG ACTCGTAGCA TCTCTCTGCG AAAAGCCAGG 
AAGCCCCCAC TCCCTCCAGA AAGAACCGAC TCTTTAAGGC GTAAACCCCA GAGGAAGAGT 
CGCCACAGTG GTTCAGTCCT TAATGAGCAG TTAATCTCTA GTCTCCAGCA GTCCTTACAA 
CTTAACCTAA ATACTCAAAC TACATCTTGC TCTGAACAGT CACCCTGTGG TAGGTTTGAA 
GACCCTTGGG TCCTCCGACC GAGAAGTCAG AGTATCATAA GTGCAGCTAG CAGTGGGGTA 
TCTGCACCAG CTGCTGTATG TCCGGTCACA CCAACACACA GTGACAGCAG CAGTCAGCGC 
TCAGACTATG CAGAGTCATG GGACTTCTAC ATGGATTGCC CATGTCCACG GTCTGACCAG 
AGTCTTTCCT CACAAATCAC AAGGTCTGCA AGTGCTGAGG AGAATCATGG AGTCATAGGT 
AATGCATCCT ACAACAGTAA ATTCCCATCA TCCCCCTTCA GCAATAGTCA GTCAAAATTG 
ACCTCATCTC CTGACAAAGT CCATCGACTC ACGTCACCAT CAAGTGGCTA CTCAAGTCAG 
TCTAACACTC CAACTGCAGG CACTCCAGTT ACGTCTCTAA TAAGAGCAAA GTCTCCTGCG 
GGGAGACCGA AACCAAAAGT GCCAGAAAGA AAGTCCTCTC TACGATCTTC TATATCATCC 
TCTTCTACAT CCCTGTCCTC CAACACCTCA GATTCCATCA GGAACATTCC TCCACCTCCA 
CCTTTACCTG ACATGACAAC TGCATTATGT GGGTCGCCCA GTTCTGCTTC TTTCTCTCCA 
ACAGCGGCTA AAATAGACCC AATACCCTCT TTTACATCTC TACAGGATGT ATCATCTGCC 
TTGGAAGATG GGCAAAATAC ACTTTCAGAT ACATCTGCAA CACCTGAGGA ATGTGATAGT 
CCTGATTTTC CCCCTCCCCA TTCAAATGTT TTTGAATCTC TGTCAGGCAA TGTAGATACC 
AACAGCCTCC CACCTCCTCC ACTTCTGCTT TGCAAACCTT CACTGTGCAT TTCATCTCTT 
CTTCCCCCTC CTCCTCCACC TCCTCCACTA TTACTTGAGA CCAGTCTTCA ACCAGCCACC 
ATTTTGAAGA CAGAGAGCAG TCTTACACCT ACAACAGCTG TAAAATGTGA AAAAGAAGTA 
AACATCAGTC ACACTGGAGA GCAAGAAAAG ACTCAGAGAC CAATAATCAC TGCCCAGGCC 
CTTCAGATGG TGCAGTTGCG ACCTGTTAAG CTCAAGCAAA TAGAAACTAT TTTTACATCT 
GTCAGTTTTG ATCATTCTGA TCACCAAGCA CAAAACCATT CTGATACAAA CTCAGGAAAG 
TCCCTGGAAA TTGATAATAC AGGGCAAACA GTGACCGTCA ATTGTGTTAC TCAGTCAGAA 
AATAGTATGG AAACATCTGA ATGTGAATAT TCGGTGAACA GATTCATAGA CGATCTCCCT 
GAGGTATCAA CTAGCACTCT ACCCCCTGAC CTAGATGAAC AATTAAAAAA AACTGAGCAA 
AATAATCCAG TGCACCTCTC TTCCAAGTCC ACAAATTCCT TAGCTTCTCC TCTGAGTTCT 
CCAATGAAAC AGAAGCCTCC TATATCACCC AAAAAACCCA ATCTTTCCCT TATTATACCA 
CCCATAATGC ACTTGCCATT TTCCAACGGA CTCAAGCTTC AGCAGGTTCA TGAAGCACAA 
GAGACACTAG AGTCAGAGCA AGTGAATGGT GCATCCTCGA CAAACACAGT TAATTTGCAA 
GCAATTCCTT TAGACCTTGA GGTAGAGGAA GAGAGTGACT CAGACTTATC AACCCCTGTG 
GTCAAGAGCA GACTATCTTT CTGCAGCGAG TTATCATCAA GCAGTTTCCA AGACCTGCAA 
CTTTCTGATT ACATTCTTAA TGAGCATGAC CTCGGCCTGA GCGATAAAGA CTTTGGGCTC 
TGTGATGATA AAAGCACATC AGATGATGGT TCAAGCAGCA GCTCAGGATC CATCAGCTTT 
AAAGAGGATG AACATGAGGA AAACGGTGCT GTGTTTGACT CAATCACTGA AAGCACATCT 
GACATTAACG CTAGCGGAGA AGCTGTGGAG GGGATGGTGA CGCCTGTTCG CCCTCGTACT 
ACAGAGGACC TCTTTGCTGC AATTCATAGG TCAAAAAGGA AGGTCCTGGG CCGTGGCGAT 
TCTGAAGATG AACGCTGTCG TAACCTCCTC CCTTCCCCAC CTGTCACTCC AACTGGCTCC 
TGCCCCAGCT TGCCACGTCA GACCAGCTCC ATCCAGCGGA ACCTACGTCG CTCCACCACA 
AGCAATGACA GTTTTAAAGC TTTGCTACTC AAGAAGGGCA GCCGCTCAGA GTCCAGCTTT 
CGCATGTCAG CCGCTGAGAT TCTCAAATGC ACAGACCCCC GTTTCCAAAG GGCTAATTCA 
GAGAATGCAC TAGCTGACGG ACTGTCCTCT CCGACAAGCA ACAGGAGAGC ACATGAAGAG 
TGGGCACGGA TGGAGGGAGC TTTGCCTCGC TTTACCACTG GACAAACTAT CCCAAAATAT 
GGGCGCTCGC GCACACCACC TTCCGCAGCC AGCAGCCGGT ATAACAGCCG CAGCCGGATT 
ACCAGTAGGC CAATGACTGC CATCTGTGAA CGAGATGGAG AGGTGGCTGA ATCTACAGAC 
TGCTACATAA GTAGCGAGAA TCATTTTGTC TTGGCTTTAT CCTCCAGCAG CATGGTCTGC 
GCTCAAGGCA GCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158876.2
CDS	280..5097
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158819.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158819.4

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGCGG TTTAAAGTCC TAGACTGCCT ATCACAGTCT 
TGCCTCTCTC TCTGCTGTCA TTTGAGATCT TGGAAAACAA AGGCTTCCGA TTCACCTAAT 
GAAGAGGATA AACTTGTGTA CTCAATGCCG CCACAAACCC CTCTGCTGGA GCATGGCTCT 
GACATGGACA TCCAGAGAAG CTGGACCAGC TGTCTACCTT TGCCCACTCC CGAGGAGAAG 
ATGAGACTGC AGGCCCAATC GGTGGCTACT GATATAATTC CTATCAACAT TACAGGTGAG 
AACTTTGACC GCCAGGCCAG CTTTCGCCGC TCCATGGCAA ACACTGATAC TATTATACGA 
AGACCTAAGA GAGTTCAAAG AAGAAAGACA ATAACAGGAG TGCCTGACAA CATCCAGACT 
GAACTAGCTA ATAAAGAACA AGCTGACTCC AGAATACATT CGAGTATGGC AGATCCGTAC 
TCCACCCTCA GTCGAGTGGG AAGTGTAAAC TCCACCCTGA AACGCTCAGA CACCCGTGAT 
TCCTGTTGTC AAACAGAAGA GGTGAAGATT GTTCCTCCCT CAGTACGCAG AATCAGAGCA 
CAAAGAGGCC ATGGAATAGC TGCCCAAATG GCCAGTGTCT CCTCCTCAAG CAGCATTTCC 
ACCATAAGTG ACTGTAATGG CATTAGCTAC ACACAGCTTA ATGACAGTGA TCAGAGCTTT 
CAAAGTTTAC CAAGACAAGG TTCTCGCATC CCGCAACAAC ACAGTGAAAC AAAGTACAAT 
AGCTCCCCCT ACAGGGCGAA TTCTGGTTCT ACAAGCCCCT TGTCCTACCA TTTTAACATG 
CAGCATGACT CTTCATCATC GCTGCATATG CTGTCTGACT CGAGAAGCAA GCTTACTGAA 
AATCTAAATT ACGAAGGCTC TGATAAAAAT AATACCTCAA GCTTGTTTAG TCTCAGTGGT 
GCTCTACAGA ACTCCATTCC TTATGGATAC AAAAGGACAG AGGACAACCA AATGCAATCA 
CCTCATTCGT CAGTCCAGTT GCTCAATTCT GAGGATGCCT CATTGTCGGG TGTCTCCTCG 
TGTTATGAAT CCACAGCGTC CCTCTGTACA GGTATTCATA CAGAGACTGA GTCCCAGTGC 
AGCACACTTG ATGGCAGAAA CTGCTGCAGT CCTAACTGTG TCCGCAGGGA TTCCAACTTC 
TCAGAGAGCA GCAACCAAAG CTGCAGCACA CTAACCACGG ACCAATGGAC GTATGAAGTT 
TCTCCAAAAG ACAATGTTAC TTCAAGTTGC TCCTCACCTG TCAGCCATAT ATACCACGCA 
GAGGAACATT CTCCCAGCAA GACAGATACA AGCTCATTGT TCTCTGTTGA CAATGAAGGT 
TACTACACCT CCATGCGCCT AGACTCTGGT TTGAAGTCAC ACAGTCACGG CTGCATCAAT 
AAAGCAGGAG AACAAAAGCA CAGTTTGTAT GAGTGCAAAG ACCACCACAG TCAAAGTGAT 
ACTGCAAGTC TGCATAGCAA CCGCTCCCTG ACTCGTAGCA TCTCTCTGCG AAAAGCCAGG 
AAGCCCCCAC TCCCTCCAGA AAGAACCGAC TCTTTAAGGC GTAAACCCCA GAGGAAGAGT 
CGCCACAGTG GTTCAGTCCT TAATGAGCAG TTAATCTCTA GTCTCCAGCA GTCCTTACAA 
CTTAACCTAA ATACTCAAAC TACATCTTGC TCTGAACAGT CACCCTGTGG TAGGTTTGAA 
GACCCTTGGG TCCTCCGACC GAGAAGTCAG AGTATCATAA GTGCAGCTAG CAGTGGGGTA 
TCTGCACCAG CTGCTGTATG TCCGGTCACA CCAACACACA GTGACAGCAG CAGTCAGCGC 
TCAGACTATG CAGAGTCATG GGACTTCTAC ATGGATTGCC CATGTCCACG GTCTGACCAG 
AGTCTTTCCT CACAAATCAC AAGGTCTGCA AGTGCTGAGG AGAATCATGG AGTCATAGGT 
AATGCATCCT ACAACAGTAA ATTCCCATCA TCCCCCTTCA GCAATAGTCA GTCAAAATTG 
ACCTCATCTC CTGACAAAGT CCATCGACTC ACGTCACCAT CAAGTGGCTA CTCAAGTCAG 
TCTAACACTC CAACTGCAGG CACTCCAGTT ACGTCTCTAA TAAGAGCAAA GTCTCCTGCG 
GGGAGACCGA AACCAAAAGT GCCAGAAAGA AAGTCCTCTC TACGATCTTC TATATCATCC 
TCTTCTACAT CCCTGTCCTC CAACACCTCA GATTCCATCA GGAACATTCC TCCACCTCCA 
CCTTTACCTG ACATGACAAC TGCATTATGT GGGTCGCCCA GTTCTGCTTC TTTCTCTCCA 
ACAGCGGCTA AAATAGACCC AATACCCTCT TTTACATCTC TACAGGATGT ATCATCTGCC 
TTGGAAGATG GGCAAAATAC ACTTTCAGAT ACATCTGCAA CACCTGAGGA ATGTGATAGT 
CCTGATTTTC CCCCTCCCCA TTCAAATGTT TTTGAATCTC TGTCAGGCAA TGTAGATACC 
AACAGCCTCC CACCTCCTCC ACTTCTGCTT TGCAAACCTT CACTGTGCAT TTCATCTCTT 
CTTCCCCCTC CTCCTCCACC TCCTCCACTA TTACTTGAGA CCAGTCTTCA ACCAGCCACC 
ATTTTGAAGA CAGAGAGCAG TCTTACACCT ACAACAGCTG TAAAATGTGA AAAAGAAGTA 
AACATCAGTC ACACTGGAGA GCAAGAAAAG ACTCAGAGAC CAATAATCAC TGCCCAGGCC 
CTTCAGATGG TGCAGTTGCG ACCTGTTAAG CTCAAGCAAA TAGAAACTAT TTTTACATCT 
GTCAGTTTTG ATCATTCTGA TCACCAAGCA CAAAACCATT CTGATACAAA CTCAGGAAAG 
TCCCTGGAAA TTGATAATAC AGGGCAAACA GTGACCGTCA ATTGTGTTAC TCAGTCAGAA 
AATAGTATGG AAACATCTGA ATGTGAATAT TCGGTGAACA GATTCATAGA CGATCTCCCT 
GAGGTATCAA CTAGCACTCT ACCCCCTGAC CTAGATGAAC AATTAAAAAA AACTGAGCAA 
AATAATCCAG TGCACCTCTC TTCCAAGTCC ACAAATTCCT TAGCTTCTCC TCTGAGTTCT 
CCAATGAAAC AGAAGCCTCC TATATCACCC AAAAAACCCA ATCTTTCCCT TATTATACCA 
CCCATAATGC ACTTGCCATT TTCCAACGGA CTCAAGCTTC AGCAGGTTCA TGAAGCACAA 
GAGACACTAG AGTCAGAGCA AGTGAATGGT GCATCCTCGA CAAACACAGT TAATTTGCAA 
GCAATTCCTT TAGACCTTGA GGTAGAGGAA GAGAGTGACT CAGACTTATC AACCCCTGTG 
GTCAAGAGCA GACTATCTTT CTGCAGCGAG TTATCATCAA GCAGTTTCCA AGACCTGCAA 
CTTTCTGATT ACATTCTTAA TGAGCATGAC CTCGGCCTGA GCGATAAAGA CTTTGGGCTC 
TGTGATGATA AAAGCACATC AGATGATGGT TCAAGCAGCA GCTCAGGATC CATCAGCTTT 
AAAGAGGATG AACATGAGGA AAACGGTGCT GTGTTTGACT CAATCACTGA AAGCACATCT 
GACATTAACG CTAGCGGAGA AGCTGTGGAG GGGATGGTGA CGCCTGTTCG CCCTCGTACT 
ACAGAGGACC TCTTTGCTGC AATTCATAGG TCAAAAAGGA AGGTCCTGGG CCGTGGCGAT 
TCTGAAGATG AACGCTGTCG TAACCTCCTC CCTTCCCCAC CTGTCACTCC AACTGGCTCC 
TGCCCCAGCT TGCCACGTCA GACCAGCTCC ATCCAGCGGA ACCTACGTCG CTCCACCACA 
AGCAATGACA GTTTTAAAGC TTTGCTACTC AAGAAGGGCA GCCGCTCAGA GTCCAGCTTT 
CGCATGTCAG CCGCTGAGAT TCTCAAATGC ACAGACCCCC GTTTCCAAAG GGCTAATTCA 
GAGAATGCAC TAGCTGACGG ACTGTCCTCT CCGACAAGCA ACAGGAGAGC ACATGAAGAG 
TGGGCACGGA TGGAGGGAGC TTTGCCTCGC TTTACCACTG GACAAACTAT CCCAAAATAT 
GGGCGCTCGC GCACACCACC TTCCGCAGCC AGCAGCCGGT ATAACAGCCG CAGCCGGATT 
ACCAGTAGGC CAATGACTGC CATCTGTGAA CGAGATGGAG AGGTGGCTGA ATCTACAGAC 
TGCTACATAA GTAGCGAGAA TCATTTTGTC TTGGCTTTAT CCTCCAGCAG CATGGTCTGC 
GCTCAAGGCA GCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158820.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158877.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145945
Clone ID Related Accession (Same CDS sequence)	XM_005158820.4
Accession Version	XM_005158820.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158820.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC ATAAGAGAAC AGTGGAGCCC CGGAGCCTGG GCAGAATAAA CAGGTATCGA 
CAGGACGTTC AGGAGGATGT GTTTTCTTCT CTGGATGCTG TGAACTGTCA GGCTTTGATG 
AACGTCCTCC GTCAGCTGTG CGATGTGTCC CGGCACGCGA GCTGCATGTT TGTGGAGCTG 
CAGACTGAAG CAGCGCTGGT CATCAATAAA GCCTCAGCGC TGCAGCACAG ACTGGACACA 
CTGCAGGACA CTGTGCGACA CCTCAACCAC AGGAGAACCA CGACCCCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGCGG TTTAAAGTCC TAGACTGCCT ATCACAGTCT 
TGCCTCTCTC TCTGCTGTCA TTTGAGATCT TGGAAAACAA AGGCTTCCGA TTCACCTAAT 
GAAGAGGATA AACTTGTGTA CTCAATGCCG CCACAAACCC CTCTGCTGGA GCATGGCTCT 
GACATGGACA TCCAGAGAAG CTGGACCAGC TGTCTACCTT TGCCCACTCC CGAGGAGAAG 
ATGAGACTGC AGGCCCAATC GGTGGCTACT GATATAATTC CTATCAACAT TACAGGTGAG 
AACTTTGACC GCCAGGCCAG CTTTCGCCGC TCCATGGCAA ACACTGATAC TATTATACGA 
AGACCTAAGA GAGTTCAAAG AAGAAAGACA ATAACAGGAG TGCCTGACAA CATCCAGACT 
GAACTAGCTA ATAAAGAACA AGCTGACTCC AGAATACATT CGAGTATGGC AGATCCGTAC 
TCCACCCTCA GTCGAGTGGG AAGTGTAAAC TCCACCCTGA AACGCTCAGA CACCCGTGAT 
TCCTGTTGTC AAACAGAAGA GGTGAAGATT GTTCCTCCCT CAGTACGCAG AATCAGAGCA 
CAAAGAGGCC ATGGAATAGC TGCCCAAATG GCCAGTGTCT CCTCCTCAAG CAGCATTTCC 
ACCATAAGTG ACTGTAATGG CATTAGCTAC ACACAGCTTA ATGACAGTGA TCAGAGCTTT 
CAAAGTTTAC CAAGACAAGG TTCTCGCATC CCGCAACAAC ACAGTGAAAC AAAGTACAAT 
AGCTCCCCCT ACAGGGCGAA TTCTGGTTCT ACAAGCCCCT TGTCCTACCA TTTTAACATG 
CAGCATGACT CTTCATCATC GCTGCATATG CTGTCTGACT CGAGAAGCAA GCTTACTGAA 
AATCTAAATT ACGAAGGCTC TGATAAAAAT AATACCTCAA GCTTGTTTAG TCTCAGTGGT 
GCTCTACAGA ACTCCATTCC TTATGGATAC AAAAGGACAG AGGACAACCA AATGCAATCA 
CCTCATTCGT CAGTCCAGTT GCTCAATTCT GAGGATGCCT CATTGTCGGG TGTCTCCTCG 
TGTTATGAAT CCACAGCGTC CCTCTGTACA GGTATTCATA CAGAGACTGA GTCCCAGTGC 
AGCACACTTG ATGGCAGAAA CTGCTGCAGT CCTAACTGTG TCCGCAGGGA TTCCAACTTC 
TCAGAGAGCA GCAACCAAAG CTGCAGCACA CTAACCACGG ACCAATGGAC GTATGAAGTT 
TCTCCAAAAG ACAATGTTAC TTCAAGTTGC TCCTCACCTG TCAGCCATAT ATACCACGCA 
GAGGAACATT CTCCCAGCAA GACAGATACA AGCTCATTGT TCTCTGTTGA CAATGAAGGT 
TACTACACCT CCATGCGCCT AGACTCTGGT TTGAAGTCAC ACAGTCACGG CTGCATCAAT 
AAAGCAGGAG AACAAAAGCA CAGTTTGTAT GAGTGCAAAG ACCACCACAG TCAAAGTGAT 
ACTGCAAGTC TGCATAGCAA CCGCTCCCTG ACTCGTAGCA TCTCTCTGCG AAAAGCCAGG 
AAGCCCCCAC TCCCTCCAGA AAGAACCGAC TCTTTAAGGC GTAAACCCCA GAGGAAGAGT 
CGCCACAGTG GTTCAGTCCT TAATGAGCAG TTAATCTCTA GTCTCCAGCA GTCCTTACAA 
CTTAACCTAA ATACTCAAAC TACATCTTGC TCTGAACAGT CACCCTGTGG TAGGTTTGAA 
GACCCTTGGG TCCTCCGACC GAGAAGTCAG AGTATCATAA GTGCAGCTAG CAGTGGGGTA 
TCTGCACCAG CTGCTGTATG TCCGGTCACA CCAACACACA GTGACAGCAG CAGTCAGCGC 
TCAGACTATG CAGAGTCATG GGACTTCTAC ATGGATTGCC CATGTCCACG GTCTGACCAG 
AGTCTTTCCT CACAAATCAC AAGGTCTGCA AGTGCTGAGG AGAATCATGG AGTCATAGGT 
AATGCATCCT ACAACAGTAA ATTCCCATCA TCCCCCTTCA GCAATAGTCA GTCAAAATTG 
ACCTCATCTC CTGACAAAGT CCATCGACTC ACGTCACCAT CAAGTGGCTA CTCAAGTCAG 
TCTAACACTC CAACTGCAGG CACTCCAGTT ACGTCTCTAA TAAGAGCAAA GTCTCCTGCG 
GGGAGACCGA AACCAAAAGT GCCAGAAAGA AAGTCCTCTC TACGATCTTC TATATCATCC 
TCTTCTACAT CCCTGTCCTC CAACACCTCA GATTCCATCA GGAACATTCC TCCACCTCCA 
CCTTTACCTG ACATGACAAC TGCATTATGT GGGTCGCCCA GTTCTGCTTC TTTCTCTCCA 
ACAGCGGCTA AAATAGACCC AATACCCTCT TTTACATCTC TACAGGATGT ATCATCTGCC 
TTGGAAGATG GGCAAAATAC ACTTTCAGAT ACATCTGCAA CACCTGAGGA ATGTGATAGT 
CCTGATTTTC CCCCTCCCCA TTCAAATGTT TTTGAATCTC TGTCAGGCAA TGTAGATACC 
AACAGCCTCC CACCTCCTCC ACTTCTGCTT TGCAAACCTT CACTGTGCAT TTCATCTCTT 
CTTCCCCCTC CTCCTCCACC TCCTCCACTA TTACTTGAGA CCAGTCTTCA ACCAGCCACC 
ATTTTGAAGA CAGAGAGCAG TCTTACACCT ACAACAGCTG TAAAATGTGA AAAAGAAGTA 
AACATCAGTC ACACTGGAGA GCAAGAAAAG ACTCAGAGAC CAATAATCAC TGCCCAGGCC 
CTTCAGATGG TGCAGTTGCG ACCTGTTAAG CTCAAGCAAA TAGAAACTAT TTTTACATCT 
GTCAGTTTTG ATCATTCTGA TCACCAAGCA CAAAACCATT CTGATACAAA CTCAGGAAAG 
TCCCTGGAAA TTGATAATAC AGGGCAAACA GTGACCGTCA ATTGTGTTAC TCAGTCAGAA 
AATAGTATGG AAACATCTGA ATGTGAATAT TCGGTGAACA GATTCATAGA CGATCTCCCT 
GAGGTATCAA CTAGCACTCT ACCCCCTGAC CTAGATGAAC AATTAAAAAA AACTGAGCAA 
AATAATCCAG TGCACCTCTC TTCCAAGTCC ACAAATTCCT TAGCTTCTCC TCTGAGTTCT 
CCAATGAAAC AGAAGCCTCC TATATCACCC AAAAAACCCA ATCTTTCCCT TATTATACCA 
CCCATAATGC ACTTGCCATT TTCCAACGGA CTCAAGCTTC AGCAGGTTCA TGAAGCACAA 
GAGACACTAG AGTCAGAGCA AGTGAATGGT GCATCCTCGA CAAACACAGT TAATTTGCAA 
GCAATTCCTT TAGACCTTGA GGTAGAGGAA GAGAGTGACT CAGACTTATC AACCCCTGTG 
GTCAAGAGCA GACTATCTTT CTGCAGCGAG TTATCATCAA GCAGTTTCCA AGACCTGCAA 
CTTTCTGATT ACATTCTTAA TGAGCATGAC CTCGGCCTGA GCGATAAAGA CTTTGGGCTC 
TGTGATGATA AAAGCACATC AGATGATGGT TCAAGCAGCA GCTCAGGATC CATCAGCTTT 
AAAGAGGATG AACATGAGGA AAACGGTGCT GTGTTTGACT CAATCACTGA AAGCACATCT 
GACATTAACG CTAGCGGAGA AGCTGTGGAG GGGATGGTGA CGCCTGTTCG CCCTCGTACT 
ACAGAGGACC TCTTTGCTGC AATTCATAGG TCAAAAAGGA AGGTCCTGGG CCGTGGCGAT 
TCTGAAGATG AACGCTGTCG TAACCTCCTC CCTTCCCCAC CTGTCACTCC AACTGGCTCC 
TGCCCCAGCT TGCCACGTCA GACCAGCTCC ATCCAGCGGA ACCTACGTCG CTCCACCACA 
AGCAATGACA GTTTTAAAGC TTTGCTACTC AAGAAGGGCA GCCGCTCAGA GTCCAGCTTT 
CGCATGTCAG CCGCTGAGAT TCTCAAATGC ACAGACCCCC GTTTCCAAAG GGCTAATTCA 
GAGAATGCAC TAGCTGACGG ACTGTCCTCT CCGACAAGCA ACAGGAGAGC ACATGAAGAG 
TGGGCACGGA TGGAGGGAGC TTTGCCTCGC TTTACCACTG GACAAACTAT CCCAAAATAT 
GGGCGCTCGC GCACACCACC TTCCGCAGCC AGCAGCCGGT ATAACAGCCG CAGCCGGATT 
ACCAGTAGGC CAATGACTGC CATCTGTGAA CGAGATGGAG AGGTGGCTGA ATCTACAGAC 
TGCTACATAA GTAGCGAGAA TCATTTTGTC TTGGCTTTAT CCTCCAGCAG CATGGTCTGC 
GCTCAAGGCA GCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158877.2
CDS	179..4996
Translation	MPFHKRTVEPRSLGRINRYRQDVQEDVFSSLDAVNCQALMNVLRQLCDVSRHASCMFVELQTEAALVINKASALQHRLDTLQDTVRHLNHRRTTTPVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158820.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158820.4

ORF Insert Sequence:

ATGCCTTTTC ATAAGAGAAC AGTGGAGCCC CGGAGCCTGG GCAGAATAAA CAGGTATCGA 
CAGGACGTTC AGGAGGATGT GTTTTCTTCT CTGGATGCTG TGAACTGTCA GGCTTTGATG 
AACGTCCTCC GTCAGCTGTG CGATGTGTCC CGGCACGCGA GCTGCATGTT TGTGGAGCTG 
CAGACTGAAG CAGCGCTGGT CATCAATAAA GCCTCAGCGC TGCAGCACAG ACTGGACACA 
CTGCAGGACA CTGTGCGACA CCTCAACCAC AGGAGAACCA CGACCCCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGCGG TTTAAAGTCC TAGACTGCCT ATCACAGTCT 
TGCCTCTCTC TCTGCTGTCA TTTGAGATCT TGGAAAACAA AGGCTTCCGA TTCACCTAAT 
GAAGAGGATA AACTTGTGTA CTCAATGCCG CCACAAACCC CTCTGCTGGA GCATGGCTCT 
GACATGGACA TCCAGAGAAG CTGGACCAGC TGTCTACCTT TGCCCACTCC CGAGGAGAAG 
ATGAGACTGC AGGCCCAATC GGTGGCTACT GATATAATTC CTATCAACAT TACAGGTGAG 
AACTTTGACC GCCAGGCCAG CTTTCGCCGC TCCATGGCAA ACACTGATAC TATTATACGA 
AGACCTAAGA GAGTTCAAAG AAGAAAGACA ATAACAGGAG TGCCTGACAA CATCCAGACT 
GAACTAGCTA ATAAAGAACA AGCTGACTCC AGAATACATT CGAGTATGGC AGATCCGTAC 
TCCACCCTCA GTCGAGTGGG AAGTGTAAAC TCCACCCTGA AACGCTCAGA CACCCGTGAT 
TCCTGTTGTC AAACAGAAGA GGTGAAGATT GTTCCTCCCT CAGTACGCAG AATCAGAGCA 
CAAAGAGGCC ATGGAATAGC TGCCCAAATG GCCAGTGTCT CCTCCTCAAG CAGCATTTCC 
ACCATAAGTG ACTGTAATGG CATTAGCTAC ACACAGCTTA ATGACAGTGA TCAGAGCTTT 
CAAAGTTTAC CAAGACAAGG TTCTCGCATC CCGCAACAAC ACAGTGAAAC AAAGTACAAT 
AGCTCCCCCT ACAGGGCGAA TTCTGGTTCT ACAAGCCCCT TGTCCTACCA TTTTAACATG 
CAGCATGACT CTTCATCATC GCTGCATATG CTGTCTGACT CGAGAAGCAA GCTTACTGAA 
AATCTAAATT ACGAAGGCTC TGATAAAAAT AATACCTCAA GCTTGTTTAG TCTCAGTGGT 
GCTCTACAGA ACTCCATTCC TTATGGATAC AAAAGGACAG AGGACAACCA AATGCAATCA 
CCTCATTCGT CAGTCCAGTT GCTCAATTCT GAGGATGCCT CATTGTCGGG TGTCTCCTCG 
TGTTATGAAT CCACAGCGTC CCTCTGTACA GGTATTCATA CAGAGACTGA GTCCCAGTGC 
AGCACACTTG ATGGCAGAAA CTGCTGCAGT CCTAACTGTG TCCGCAGGGA TTCCAACTTC 
TCAGAGAGCA GCAACCAAAG CTGCAGCACA CTAACCACGG ACCAATGGAC GTATGAAGTT 
TCTCCAAAAG ACAATGTTAC TTCAAGTTGC TCCTCACCTG TCAGCCATAT ATACCACGCA 
GAGGAACATT CTCCCAGCAA GACAGATACA AGCTCATTGT TCTCTGTTGA CAATGAAGGT 
TACTACACCT CCATGCGCCT AGACTCTGGT TTGAAGTCAC ACAGTCACGG CTGCATCAAT 
AAAGCAGGAG AACAAAAGCA CAGTTTGTAT GAGTGCAAAG ACCACCACAG TCAAAGTGAT 
ACTGCAAGTC TGCATAGCAA CCGCTCCCTG ACTCGTAGCA TCTCTCTGCG AAAAGCCAGG 
AAGCCCCCAC TCCCTCCAGA AAGAACCGAC TCTTTAAGGC GTAAACCCCA GAGGAAGAGT 
CGCCACAGTG GTTCAGTCCT TAATGAGCAG TTAATCTCTA GTCTCCAGCA GTCCTTACAA 
CTTAACCTAA ATACTCAAAC TACATCTTGC TCTGAACAGT CACCCTGTGG TAGGTTTGAA 
GACCCTTGGG TCCTCCGACC GAGAAGTCAG AGTATCATAA GTGCAGCTAG CAGTGGGGTA 
TCTGCACCAG CTGCTGTATG TCCGGTCACA CCAACACACA GTGACAGCAG CAGTCAGCGC 
TCAGACTATG CAGAGTCATG GGACTTCTAC ATGGATTGCC CATGTCCACG GTCTGACCAG 
AGTCTTTCCT CACAAATCAC AAGGTCTGCA AGTGCTGAGG AGAATCATGG AGTCATAGGT 
AATGCATCCT ACAACAGTAA ATTCCCATCA TCCCCCTTCA GCAATAGTCA GTCAAAATTG 
ACCTCATCTC CTGACAAAGT CCATCGACTC ACGTCACCAT CAAGTGGCTA CTCAAGTCAG 
TCTAACACTC CAACTGCAGG CACTCCAGTT ACGTCTCTAA TAAGAGCAAA GTCTCCTGCG 
GGGAGACCGA AACCAAAAGT GCCAGAAAGA AAGTCCTCTC TACGATCTTC TATATCATCC 
TCTTCTACAT CCCTGTCCTC CAACACCTCA GATTCCATCA GGAACATTCC TCCACCTCCA 
CCTTTACCTG ACATGACAAC TGCATTATGT GGGTCGCCCA GTTCTGCTTC TTTCTCTCCA 
ACAGCGGCTA AAATAGACCC AATACCCTCT TTTACATCTC TACAGGATGT ATCATCTGCC 
TTGGAAGATG GGCAAAATAC ACTTTCAGAT ACATCTGCAA CACCTGAGGA ATGTGATAGT 
CCTGATTTTC CCCCTCCCCA TTCAAATGTT TTTGAATCTC TGTCAGGCAA TGTAGATACC 
AACAGCCTCC CACCTCCTCC ACTTCTGCTT TGCAAACCTT CACTGTGCAT TTCATCTCTT 
CTTCCCCCTC CTCCTCCACC TCCTCCACTA TTACTTGAGA CCAGTCTTCA ACCAGCCACC 
ATTTTGAAGA CAGAGAGCAG TCTTACACCT ACAACAGCTG TAAAATGTGA AAAAGAAGTA 
AACATCAGTC ACACTGGAGA GCAAGAAAAG ACTCAGAGAC CAATAATCAC TGCCCAGGCC 
CTTCAGATGG TGCAGTTGCG ACCTGTTAAG CTCAAGCAAA TAGAAACTAT TTTTACATCT 
GTCAGTTTTG ATCATTCTGA TCACCAAGCA CAAAACCATT CTGATACAAA CTCAGGAAAG 
TCCCTGGAAA TTGATAATAC AGGGCAAACA GTGACCGTCA ATTGTGTTAC TCAGTCAGAA 
AATAGTATGG AAACATCTGA ATGTGAATAT TCGGTGAACA GATTCATAGA CGATCTCCCT 
GAGGTATCAA CTAGCACTCT ACCCCCTGAC CTAGATGAAC AATTAAAAAA AACTGAGCAA 
AATAATCCAG TGCACCTCTC TTCCAAGTCC ACAAATTCCT TAGCTTCTCC TCTGAGTTCT 
CCAATGAAAC AGAAGCCTCC TATATCACCC AAAAAACCCA ATCTTTCCCT TATTATACCA 
CCCATAATGC ACTTGCCATT TTCCAACGGA CTCAAGCTTC AGCAGGTTCA TGAAGCACAA 
GAGACACTAG AGTCAGAGCA AGTGAATGGT GCATCCTCGA CAAACACAGT TAATTTGCAA 
GCAATTCCTT TAGACCTTGA GGTAGAGGAA GAGAGTGACT CAGACTTATC AACCCCTGTG 
GTCAAGAGCA GACTATCTTT CTGCAGCGAG TTATCATCAA GCAGTTTCCA AGACCTGCAA 
CTTTCTGATT ACATTCTTAA TGAGCATGAC CTCGGCCTGA GCGATAAAGA CTTTGGGCTC 
TGTGATGATA AAAGCACATC AGATGATGGT TCAAGCAGCA GCTCAGGATC CATCAGCTTT 
AAAGAGGATG AACATGAGGA AAACGGTGCT GTGTTTGACT CAATCACTGA AAGCACATCT 
GACATTAACG CTAGCGGAGA AGCTGTGGAG GGGATGGTGA CGCCTGTTCG CCCTCGTACT 
ACAGAGGACC TCTTTGCTGC AATTCATAGG TCAAAAAGGA AGGTCCTGGG CCGTGGCGAT 
TCTGAAGATG AACGCTGTCG TAACCTCCTC CCTTCCCCAC CTGTCACTCC AACTGGCTCC 
TGCCCCAGCT TGCCACGTCA GACCAGCTCC ATCCAGCGGA ACCTACGTCG CTCCACCACA 
AGCAATGACA GTTTTAAAGC TTTGCTACTC AAGAAGGGCA GCCGCTCAGA GTCCAGCTTT 
CGCATGTCAG CCGCTGAGAT TCTCAAATGC ACAGACCCCC GTTTCCAAAG GGCTAATTCA 
GAGAATGCAC TAGCTGACGG ACTGTCCTCT CCGACAAGCA ACAGGAGAGC ACATGAAGAG 
TGGGCACGGA TGGAGGGAGC TTTGCCTCGC TTTACCACTG GACAAACTAT CCCAAAATAT 
GGGCGCTCGC GCACACCACC TTCCGCAGCC AGCAGCCGGT ATAACAGCCG CAGCCGGATT 
ACCAGTAGGC CAATGACTGC CATCTGTGAA CGAGATGGAG AGGTGGCTGA ATCTACAGAC 
TGCTACATAA GTAGCGAGAA TCATTTTGTC TTGGCTTTAT CCTCCAGCAG CATGGTCTGC 
GCTCAAGGCA GCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158821.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158878.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.

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CloneID	ODa145946
Clone ID Related Accession (Same CDS sequence)	XM_005158821.4
Accession Version	XM_005158821.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158821.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGGCT TCCGATTCAC CTAATGAAGA GGATAAACTT 
GTGTACTCAA TGCCGCCACA AACCCCTCTG CTGGAGCATG GCTCTGACAT GGACATCCAG 
AGAAGCTGGA CCAGCTGTCT ACCTTTGCCC ACTCCCGAGG AGAAGATGAG ACTGCAGGCC 
CAATCGGTGG CTACTGATAT AATTCCTATC AACATTACAG GTGAGAACTT TGACCGCCAG 
GCCAGCTTTC GCCGCTCCAT GGCAAACACT GATACTATTA TACGAAGACC TAAGAGAGTT 
CAAAGAAGAA AGACAATAAC AGGAGTGCCT GACAACATCC AGACTGAACT AGCTAATAAA 
GAACAAGCTG ACTCCAGAAT ACATTCGAGT ATGGCAGATC CGTACTCCAC CCTCAGTCGA 
GTGGGAAGTG TAAACTCCAC CCTGAAACGC TCAGACACCC GTGATTCCTG TTGTCAAACA 
GAAGAGGTGA AGATTGTTCC TCCCTCAGTA CGCAGAATCA GAGCACAAAG AGGCCATGGA 
ATAGCTGCCC AAATGGCCAG TGTCTCCTCC TCAAGCAGCA TTTCCACCAT AAGTGACTGT 
AATGGCATTA GCTACACACA GCTTAATGAC AGTGATCAGA GCTTTCAAAG TTTACCAAGA 
CAAGGTTCTC GCATCCCGCA ACAACACAGT GAAACAAAGT ACAATAGCTC CCCCTACAGG 
GCGAATTCTG GTTCTACAAG CCCCTTGTCC TACCATTTTA ACATGCAGCA TGACTCTTCA 
TCATCGCTGC ATATGCTGTC TGACTCGAGA AGCAAGCTTA CTGAAAATCT AAATTACGAA 
GGCTCTGATA AAAATAATAC CTCAAGCTTG TTTAGTCTCA GTGGTGCTCT ACAGAACTCC 
ATTCCTTATG GATACAAAAG GACAGAGGAC AACCAAATGC AATCACCTCA TTCGTCAGTC 
CAGTTGCTCA ATTCTGAGGA TGCCTCATTG TCGGGTGTCT CCTCGTGTTA TGAATCCACA 
GCGTCCCTCT GTACAGGTAT TCATACAGAG ACTGAGTCCC AGTGCAGCAC ACTTGATGGC 
AGAAACTGCT GCAGTCCTAA CTGTGTCCGC AGGGATTCCA ACTTCTCAGA GAGCAGCAAC 
CAAAGCTGCA GCACACTAAC CACGGACCAA TGGACGTATG AAGTTTCTCC AAAAGACAAT 
GTTACTTCAA GTTGCTCCTC ACCTGTCAGC CATATATACC ACGCAGAGGA ACATTCTCCC 
AGCAAGACAG ATACAAGCTC ATTGTTCTCT GTTGACAATG AAGGTTACTA CACCTCCATG 
CGCCTAGACT CTGGTTTGAA GTCACACAGT CACGGCTGCA TCAATAAAGC AGGAGAACAA 
AAGCACAGTT TGTATGAGTG CAAAGACCAC CACAGTCAAA GTGATACTGC AAGTCTGCAT 
AGCAACCGCT CCCTGACTCG TAGCATCTCT CTGCGAAAAG CCAGGAAGCC CCCACTCCCT 
CCAGAAAGAA CCGACTCTTT AAGGCGTAAA CCCCAGAGGA AGAGTCGCCA CAGTGGTTCA 
GTCCTTAATG AGCAGTTAAT CTCTAGTCTC CAGCAGTCCT TACAACTTAA CCTAAATACT 
CAAACTACAT CTTGCTCTGA ACAGTCACCC TGTGGTAGGT TTGAAGACCC TTGGGTCCTC 
CGACCGAGAA GTCAGAGTAT CATAAGTGCA GCTAGCAGTG GGGTATCTGC ACCAGCTGCT 
GTATGTCCGG TCACACCAAC ACACAGTGAC AGCAGCAGTC AGCGCTCAGA CTATGCAGAG 
TCATGGGACT TCTACATGGA TTGCCCATGT CCACGGTCTG ACCAGAGTCT TTCCTCACAA 
ATCACAAGGT CTGCAAGTGC TGAGGAGAAT CATGGAGTCA TAGGTAATGC ATCCTACAAC 
AGTAAATTCC CATCATCCCC CTTCAGCAAT AGTCAGTCAA AATTGACCTC ATCTCCTGAC 
AAAGTCCATC GACTCACGTC ACCATCAAGT GGCTACTCAA GTCAGTCTAA CACTCCAACT 
GCAGGCACTC CAGTTACGTC TCTAATAAGA GCAAAGTCTC CTGCGGGGAG ACCGAAACCA 
AAAGTGCCAG AAAGAAAGTC CTCTCTACGA TCTTCTATAT CATCCTCTTC TACATCCCTG 
TCCTCCAACA CCTCAGATTC CATCAGGAAC ATTCCTCCAC CTCCACCTTT ACCTGACATG 
ACAACTGCAT TATGTGGGTC GCCCAGTTCT GCTTCTTTCT CTCCAACAGC GGCTAAAATA 
GACCCAATAC CCTCTTTTAC ATCTCTACAG GATGTATCAT CTGCCTTGGA AGATGGGCAA 
AATACACTTT CAGATACATC TGCAACACCT GAGGAATGTG ATAGTCCTGA TTTTCCCCCT 
CCCCATTCAA ATGTTTTTGA ATCTCTGTCA GGCAATGTAG ATACCAACAG CCTCCCACCT 
CCTCCACTTC TGCTTTGCAA ACCTTCACTG TGCATTTCAT CTCTTCTTCC CCCTCCTCCT 
CCACCTCCTC CACTATTACT TGAGACCAGT CTTCAACCAG CCACCATTTT GAAGACAGAG 
AGCAGTCTTA CACCTACAAC AGCTGTAAAA TGTGAAAAAG AAGTAAACAT CAGTCACACT 
GGAGAGCAAG AAAAGACTCA GAGACCAATA ATCACTGCCC AGGCCCTTCA GATGGTGCAG 
TTGCGACCTG TTAAGCTCAA GCAAATAGAA ACTATTTTTA CATCTGTCAG TTTTGATCAT 
TCTGATCACC AAGCACAAAA CCATTCTGAT ACAAACTCAG GAAAGTCCCT GGAAATTGAT 
AATACAGGGC AAACAGTGAC CGTCAATTGT GTTACTCAGT CAGAAAATAG TATGGAAACA 
TCTGAATGTG AATATTCGGT GAACAGATTC ATAGACGATC TCCCTGAGGT ATCAACTAGC 
ACTCTACCCC CTGACCTAGA TGAACAATTA AAAAAAACTG AGCAAAATAA TCCAGTGCAC 
CTCTCTTCCA AGTCCACAAA TTCCTTAGCT TCTCCTCTGA GTTCTCCAAT GAAACAGAAG 
CCTCCTATAT CACCCAAAAA ACCCAATCTT TCCCTTATTA TACCACCCAT AATGCACTTG 
CCATTTTCCA ACGGACTCAA GCTTCAGCAG GTTCATGAAG CACAAGAGAC ACTAGAGTCA 
GAGCAAGTGA ATGGTGCATC CTCGACAAAC ACAGTTAATT TGCAAGCAAT TCCTTTAGAC 
CTTGAGGTAG AGGAAGAGAG TGACTCAGAC TTATCAACCC CTGTGGTCAA GAGCAGACTA 
TCTTTCTGCA GCGAGTTATC ATCAAGCAGT TTCCAAGACC TGCAACTTTC TGATTACATT 
CTTAATGAGC ATGACCTCGG CCTGAGCGAT AAAGACTTTG GGCTCTGTGA TGATAAAAGC 
ACATCAGATG ATGGTTCAAG CAGCAGCTCA GGATCCATCA GCTTTAAAGA GGATGAACAT 
GAGGAAAACG GTGCTGTGTT TGACTCAATC ACTGAAAGCA CATCTGACAT TAACGCTAGC 
GGAGAAGCTG TGGAGGGGAT GGTGACGCCT GTTCGCCCTC GTACTACAGA GGACCTCTTT 
GCTGCAATTC ATAGGTCAAA AAGGAAGGTC CTGGGCCGTG GCGATTCTGA AGATGAACGC 
TGTCGTAACC TCCTCCCTTC CCCACCTGTC ACTCCAACTG GCTCCTGCCC CAGCTTGCCA 
CGTCAGACCA GCTCCATCCA GCGGAACCTA CGTCGCTCCA CCACAAGCAA TGACAGTTTT 
AAAGCTTTGC TACTCAAGAA GGGCAGCCGC TCAGAGTCCA GCTTTCGCAT GTCAGCCGCT 
GAGATTCTCA AATGCACAGA CCCCCGTTTC CAAAGGGCTA ATTCAGAGAA TGCACTAGCT 
GACGGACTGT CCTCTCCGAC AAGCAACAGG AGAGCACATG AAGAGTGGGC ACGGATGGAG 
GGAGCTTTGC CTCGCTTTAC CACTGGACAA ACTATCCCAA AATATGGGCG CTCGCGCACA 
CCACCTTCCG CAGCCAGCAG CCGGTATAAC AGCCGCAGCC GGATTACCAG TAGGCCAATG 
ACTGCCATCT GTGAACGAGA TGGAGAGGTG GCTGAATCTA CAGACTGCTA CATAAGTAGC 
GAGAATCATT TTGTCTTGGC TTTATCCTCC AGCAGCATGG TCTGCGCTCA AGGCAGCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158878.2
CDS	279..5021
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158821.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158821.4

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGCGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAGAAAAGAA AAAGAAGGCT TCCGATTCAC CTAATGAAGA GGATAAACTT 
GTGTACTCAA TGCCGCCACA AACCCCTCTG CTGGAGCATG GCTCTGACAT GGACATCCAG 
AGAAGCTGGA CCAGCTGTCT ACCTTTGCCC ACTCCCGAGG AGAAGATGAG ACTGCAGGCC 
CAATCGGTGG CTACTGATAT AATTCCTATC AACATTACAG GTGAGAACTT TGACCGCCAG 
GCCAGCTTTC GCCGCTCCAT GGCAAACACT GATACTATTA TACGAAGACC TAAGAGAGTT 
CAAAGAAGAA AGACAATAAC AGGAGTGCCT GACAACATCC AGACTGAACT AGCTAATAAA 
GAACAAGCTG ACTCCAGAAT ACATTCGAGT ATGGCAGATC CGTACTCCAC CCTCAGTCGA 
GTGGGAAGTG TAAACTCCAC CCTGAAACGC TCAGACACCC GTGATTCCTG TTGTCAAACA 
GAAGAGGTGA AGATTGTTCC TCCCTCAGTA CGCAGAATCA GAGCACAAAG AGGCCATGGA 
ATAGCTGCCC AAATGGCCAG TGTCTCCTCC TCAAGCAGCA TTTCCACCAT AAGTGACTGT 
AATGGCATTA GCTACACACA GCTTAATGAC AGTGATCAGA GCTTTCAAAG TTTACCAAGA 
CAAGGTTCTC GCATCCCGCA ACAACACAGT GAAACAAAGT ACAATAGCTC CCCCTACAGG 
GCGAATTCTG GTTCTACAAG CCCCTTGTCC TACCATTTTA ACATGCAGCA TGACTCTTCA 
TCATCGCTGC ATATGCTGTC TGACTCGAGA AGCAAGCTTA CTGAAAATCT AAATTACGAA 
GGCTCTGATA AAAATAATAC CTCAAGCTTG TTTAGTCTCA GTGGTGCTCT ACAGAACTCC 
ATTCCTTATG GATACAAAAG GACAGAGGAC AACCAAATGC AATCACCTCA TTCGTCAGTC 
CAGTTGCTCA ATTCTGAGGA TGCCTCATTG TCGGGTGTCT CCTCGTGTTA TGAATCCACA 
GCGTCCCTCT GTACAGGTAT TCATACAGAG ACTGAGTCCC AGTGCAGCAC ACTTGATGGC 
AGAAACTGCT GCAGTCCTAA CTGTGTCCGC AGGGATTCCA ACTTCTCAGA GAGCAGCAAC 
CAAAGCTGCA GCACACTAAC CACGGACCAA TGGACGTATG AAGTTTCTCC AAAAGACAAT 
GTTACTTCAA GTTGCTCCTC ACCTGTCAGC CATATATACC ACGCAGAGGA ACATTCTCCC 
AGCAAGACAG ATACAAGCTC ATTGTTCTCT GTTGACAATG AAGGTTACTA CACCTCCATG 
CGCCTAGACT CTGGTTTGAA GTCACACAGT CACGGCTGCA TCAATAAAGC AGGAGAACAA 
AAGCACAGTT TGTATGAGTG CAAAGACCAC CACAGTCAAA GTGATACTGC AAGTCTGCAT 
AGCAACCGCT CCCTGACTCG TAGCATCTCT CTGCGAAAAG CCAGGAAGCC CCCACTCCCT 
CCAGAAAGAA CCGACTCTTT AAGGCGTAAA CCCCAGAGGA AGAGTCGCCA CAGTGGTTCA 
GTCCTTAATG AGCAGTTAAT CTCTAGTCTC CAGCAGTCCT TACAACTTAA CCTAAATACT 
CAAACTACAT CTTGCTCTGA ACAGTCACCC TGTGGTAGGT TTGAAGACCC TTGGGTCCTC 
CGACCGAGAA GTCAGAGTAT CATAAGTGCA GCTAGCAGTG GGGTATCTGC ACCAGCTGCT 
GTATGTCCGG TCACACCAAC ACACAGTGAC AGCAGCAGTC AGCGCTCAGA CTATGCAGAG 
TCATGGGACT TCTACATGGA TTGCCCATGT CCACGGTCTG ACCAGAGTCT TTCCTCACAA 
ATCACAAGGT CTGCAAGTGC TGAGGAGAAT CATGGAGTCA TAGGTAATGC ATCCTACAAC 
AGTAAATTCC CATCATCCCC CTTCAGCAAT AGTCAGTCAA AATTGACCTC ATCTCCTGAC 
AAAGTCCATC GACTCACGTC ACCATCAAGT GGCTACTCAA GTCAGTCTAA CACTCCAACT 
GCAGGCACTC CAGTTACGTC TCTAATAAGA GCAAAGTCTC CTGCGGGGAG ACCGAAACCA 
AAAGTGCCAG AAAGAAAGTC CTCTCTACGA TCTTCTATAT CATCCTCTTC TACATCCCTG 
TCCTCCAACA CCTCAGATTC CATCAGGAAC ATTCCTCCAC CTCCACCTTT ACCTGACATG 
ACAACTGCAT TATGTGGGTC GCCCAGTTCT GCTTCTTTCT CTCCAACAGC GGCTAAAATA 
GACCCAATAC CCTCTTTTAC ATCTCTACAG GATGTATCAT CTGCCTTGGA AGATGGGCAA 
AATACACTTT CAGATACATC TGCAACACCT GAGGAATGTG ATAGTCCTGA TTTTCCCCCT 
CCCCATTCAA ATGTTTTTGA ATCTCTGTCA GGCAATGTAG ATACCAACAG CCTCCCACCT 
CCTCCACTTC TGCTTTGCAA ACCTTCACTG TGCATTTCAT CTCTTCTTCC CCCTCCTCCT 
CCACCTCCTC CACTATTACT TGAGACCAGT CTTCAACCAG CCACCATTTT GAAGACAGAG 
AGCAGTCTTA CACCTACAAC AGCTGTAAAA TGTGAAAAAG AAGTAAACAT CAGTCACACT 
GGAGAGCAAG AAAAGACTCA GAGACCAATA ATCACTGCCC AGGCCCTTCA GATGGTGCAG 
TTGCGACCTG TTAAGCTCAA GCAAATAGAA ACTATTTTTA CATCTGTCAG TTTTGATCAT 
TCTGATCACC AAGCACAAAA CCATTCTGAT ACAAACTCAG GAAAGTCCCT GGAAATTGAT 
AATACAGGGC AAACAGTGAC CGTCAATTGT GTTACTCAGT CAGAAAATAG TATGGAAACA 
TCTGAATGTG AATATTCGGT GAACAGATTC ATAGACGATC TCCCTGAGGT ATCAACTAGC 
ACTCTACCCC CTGACCTAGA TGAACAATTA AAAAAAACTG AGCAAAATAA TCCAGTGCAC 
CTCTCTTCCA AGTCCACAAA TTCCTTAGCT TCTCCTCTGA GTTCTCCAAT GAAACAGAAG 
CCTCCTATAT CACCCAAAAA ACCCAATCTT TCCCTTATTA TACCACCCAT AATGCACTTG 
CCATTTTCCA ACGGACTCAA GCTTCAGCAG GTTCATGAAG CACAAGAGAC ACTAGAGTCA 
GAGCAAGTGA ATGGTGCATC CTCGACAAAC ACAGTTAATT TGCAAGCAAT TCCTTTAGAC 
CTTGAGGTAG AGGAAGAGAG TGACTCAGAC TTATCAACCC CTGTGGTCAA GAGCAGACTA 
TCTTTCTGCA GCGAGTTATC ATCAAGCAGT TTCCAAGACC TGCAACTTTC TGATTACATT 
CTTAATGAGC ATGACCTCGG CCTGAGCGAT AAAGACTTTG GGCTCTGTGA TGATAAAAGC 
ACATCAGATG ATGGTTCAAG CAGCAGCTCA GGATCCATCA GCTTTAAAGA GGATGAACAT 
GAGGAAAACG GTGCTGTGTT TGACTCAATC ACTGAAAGCA CATCTGACAT TAACGCTAGC 
GGAGAAGCTG TGGAGGGGAT GGTGACGCCT GTTCGCCCTC GTACTACAGA GGACCTCTTT 
GCTGCAATTC ATAGGTCAAA AAGGAAGGTC CTGGGCCGTG GCGATTCTGA AGATGAACGC 
TGTCGTAACC TCCTCCCTTC CCCACCTGTC ACTCCAACTG GCTCCTGCCC CAGCTTGCCA 
CGTCAGACCA GCTCCATCCA GCGGAACCTA CGTCGCTCCA CCACAAGCAA TGACAGTTTT 
AAAGCTTTGC TACTCAAGAA GGGCAGCCGC TCAGAGTCCA GCTTTCGCAT GTCAGCCGCT 
GAGATTCTCA AATGCACAGA CCCCCGTTTC CAAAGGGCTA ATTCAGAGAA TGCACTAGCT 
GACGGACTGT CCTCTCCGAC AAGCAACAGG AGAGCACATG AAGAGTGGGC ACGGATGGAG 
GGAGCTTTGC CTCGCTTTAC CACTGGACAA ACTATCCCAA AATATGGGCG CTCGCGCACA 
CCACCTTCCG CAGCCAGCAG CCGGTATAAC AGCCGCAGCC GGATTACCAG TAGGCCAATG 
ACTGCCATCT GTGAACGAGA TGGAGAGGTG GCTGAATCTA CAGACTGCTA CATAAGTAGC 
GAGAATCATT TTGTCTTGGC TTTATCCTCC AGCAGCATGG TCTGCGCTCA AGGCAGCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_009293150.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291425.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.

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CloneID	ODa145947
Clone ID Related Accession (Same CDS sequence)	XM_009293150.3
Accession Version	XM_009293150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGAG AGAGGAGTTT TCTGAGGTAC AGGGGGGAGT GGGCAGAGGG TAAGAGTCAC 
CCTGTACCTC GAAACATTGC TGCCATGGGT CTTTCTTCAT TGACAGAGCA GCGTGGACGC 
TTCTTTCCCT GCCACAAAGA TGACACTGAT GACGATTATG ATGAGTGGGT TTATGCACCT 
CTCCATCGCA GAGCGGTGTC CAGTCTTGAT GAGGAGAGCA AATGGTCGGT GCACTACACA 
GCCCCCTGGC ACCACCAAGA GAACGTCTTC CTGCCTGGGT CCAGACCAGC ATGTGTTGAG 
GATCTTCACC GTCAGGCCAA AGTCAACCTG AAAACAGTCC TCAGAGAGTG CGACAAGCTC 
AGAAAAGATG GCTTTCGCAG TTCTCAGTAT TACTCCCAAA GCCCAGCATT CTCTGCAACC 
AGCCTTTGTG AAAGTGTACA TCTGGATGAA GAGAAGACAG AAAAGAAAAA GAAGCGGTTT 
AAAGTCCTAG ACTGCCTATC ACAGTCTTGC CTCTCTCTCT GCTGTCATTT GAGATCTTGG 
AAAACAAAGG CTTCCGATTC ACCTAATGAA GAGGATAAAC TTGTGTACTC AATGCCGCCA 
CAAACCCCTC TGCTGGAGCA TGGCTCTGAC ATGGACATCC AGAGAAGCTG GACCAGCTGT 
CTACCTTTGC CCACTCCCGA GGAGAAGATG AGACTGCAGG CCCAATCGGT GGCTACTGAT 
ATAATTCCTA TCAACATTAC AGGTGAGAAC TTTGACCGCC AGGCCAGCTT TCGCCGCTCC 
ATGGCAAACA CTGATACTAT TATACGAAGA CCTAAGAGAG TTCAAAGAAG AAAGACAATA 
ACAGGAGTGC CTGACAACAT CCAGACTGAA CTAGCTAATA AAGAACAAGC TGACTCCAGA 
ATACATTCGA GTATGGCAGA TCCGTACTCC ACCCTCAGTC GAGTGGGAAG TGTAAACTCC 
ACCCTGAAAC GCTCAGACAC CCGTGATTCC TGTTGTCAAA CAGAAGAGGT GAAGATTGTT 
CCTCCCTCAG TACGCAGAAT CAGAGCACAA AGAGGCCATG GAATAGCTGC CCAAATGGCC 
AGTGTCTCCT CCTCAAGCAG CATTTCCACC ATAAGTGACT GTAATGGCAT TAGCTACACA 
CAGCTTAATG ACAGTGATCA GAGCTTTCAA AGTTTACCAA GACAAGGTTC TCGCATCCCG 
CAACAACACA GTGAAACAAA GTACAATAGC TCCCCCTACA GGGCGAATTC TGGTTCTACA 
AGCCCCTTGT CCTACCATTT TAACATGCAG CATGACTCTT CATCATCGCT GCATATGCTG 
TCTGACTCGA GAAGCAAGCT TACTGAAAAT CTAAATTACG AAGGCTCTGA TAAAAATAAT 
ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT CTACAGAACT CCATTCCTTA TGGATACAAA 
AGGACAGAGG ACAACCAAAT GCAATCACCT CATTCGTCAG TCCAGTTGCT CAATTCTGAG 
GATGCCTCAT TGTCGGGTGT CTCCTCGTGT TATGAATCCA CAGCGTCCCT CTGTACAGGT 
ATTCATACAG AGACTGAGTC CCAGTGCAGC ACACTTGATG GCAGAAACTG CTGCAGTCCT 
AACTGTGTCC GCAGGGATTC CAACTTCTCA GAGAGCAGCA ACCAAAGCTG CAGCACACTA 
ACCACGGACC AATGGACGTA TGAAGTTTCT CCAAAAGACA ATGTTACTTC AAGTTGCTCC 
TCACCTGTCA GCCATATATA CCACGCAGAG GAACATTCTC CCAGCAAGAC AGATACAAGC 
TCATTGTTCT CTGTTGACAA TGAAGGTTAC TACACCTCCA TGCGCCTAGA CTCTGGTTTG 
AAGTCACACA GTCACGGCTG CATCAATAAA GCAGGAGAAC AAAAGCACAG TTTGTATGAG 
TGCAAAGACC ACCACAGTCA AAGTGATACT GCAAGTCTGC ATAGCAACCG CTCCCTGACT 
CGTAGCATCT CTCTGCGAAA AGCCAGGAAG CCCCCACTCC CTCCAGAAAG AACCGACTCT 
TTAAGGCGTA AACCCCAGAG GAAGAGTCGC CACAGTGGTT CAGTCCTTAA TGAGCAGTTA 
ATCTCTAGTC TCCAGCAGTC CTTACAACTT AACCTAAATA CTCAAACTAC ATCTTGCTCT 
GAACAGTCAC CCTGTGGTAG GTTTGAAGAC CCTTGGGTCC TCCGACCGAG AAGTCAGAGT 
ATCATAAGTG CAGCTAGCAG TGGGGTATCT GCACCAGCTG CTGTATGTCC GGTCACACCA 
ACACACAGTG ACAGCAGCAG TCAGCGCTCA GACTATGCAG AGTCATGGGA CTTCTACATG 
GATTGCCCAT GTCCACGGTC TGACCAGAGT CTTTCCTCAC AAATCACAAG GTCTGCAAGT 
GCTGAGGAGA ATCATGGAGT CATAGGTAAT GCATCCTACA ACAGTAAATT CCCATCATCC 
CCCTTCAGCA ATAGTCAGTC AAAATTGACC TCATCTCCTG ACAAAGTCCA TCGACTCACG 
TCACCATCAA GTGGCTACTC AAGTCAGTCT AACACTCCAA CTGCAGGCAC TCCAGTTACG 
TCTCTAATAA GAGCAAAGTC TCCTGCGGGG AGACCGAAAC CAAAAGTGCC AGAAAGAAAG 
TCCTCTCTAC GATCTTCTAT ATCATCCTCT TCTACATCCC TGTCCTCCAA CACCTCAGAT 
TCCATCAGGA ACATTCCTCC ACCTCCACCT TTACCTGACA TGACAACTGC ATTATGTGGG 
TCGCCCAGTT CTGCTTCTTT CTCTCCAACA GCGGCTAAAA TAGACCCAAT ACCCTCTTTT 
ACATCTCTAC AGGATGTATC ATCTGCCTTG GAAGATGGGC AAAATACACT TTCAGATACA 
TCTGCAACAC CTGAGGAATG TGATAGTCCT GATTTTCCCC CTCCCCATTC AAATGTTTTT 
GAATCTCTGT CAGGCAATGT AGATACCAAC AGCCTCCCAC CTCCTCCACT TCTGCTTTGC 
AAACCTTCAC TGTGCATTTC ATCTCTTCTT CCCCCTCCTC CTCCACCTCC TCCACTATTA 
CTTGAGACCA GTCTTCAACC AGCCACCATT TTGAAGACAG AGAGCAGTCT TACACCTACA 
ACAGCTGTAA AATGTGAAAA AGAAGTAAAC ATCAGTCACA CTGGAGAGCA AGAAAAGACT 
CAGAGACCAA TAATCACTGC CCAGGCCCTT CAGATGGTGC AGTTGCGACC TGTTAAGCTC 
AAGCAAATAG AAACTATTTT TACATCTGTC AGTTTTGATC ATTCTGATCA CCAAGCACAA 
AACCATTCTG ATACAAACTC AGGAAAGTCC CTGGAAATTG ATAATACAGG GCAAACAGTG 
ACCGTCAATT GTGTTACTCA GTCAGAAAAT AGTATGGAAA CATCTGAATG TGAATATTCG 
GTGAACAGAT TCATAGACGA TCTCCCTGAG GTATCAACTA GCACTCTACC CCCTGACCTA 
GATGAACAAT TAAAAAAAAC TGAGCAAAAT AATCCAGTGC ACCTCTCTTC CAAGTCCACA 
AATTCCTTAG CTTCTCCTCT GAGTTCTCCA ATGAAACAGA AGCCTCCTAT ATCACCCAAA 
AAACCCAATC TTTCCCTTAT TATACCACCC ATAATGCACT TGCCATTTTC CAACGGACTC 
AAGCTTCAGC AGGTTCATGA AGCACAAGAG ACACTAGAGT CAGAGCAAGT GAATGGTGCA 
TCCTCGACAA ACACAGTTAA TTTGCAAGCA ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG 
AGTGACTCAG ACTTATCAAC CCCTGTGGTC AAGAGCAGAC TATCTTTCTG CAGCGAGTTA 
TCATCAAGCA GTTTCCAAGA CCTGCAACTT TCTGATTACA TTCTTAATGA GCATGACCTC 
GGCCTGAGCG ATAAAGACTT TGGGCTCTGT GATGATAAAA GCACATCAGA TGATGGTTCA 
AGCAGCAGCT CAGGATCCAT CAGCTTTAAA GAGGATGAAC ATGAGGAAAA CGGTGCTGTG 
TTTGACTCAA TCACTGAAAG CACATCTGAC ATTAACGCTA GCGGAGAAGC TGTGGAGGGG 
ATGGTGACGC CTGTTCGCCC TCGTACTACA GAGGACCTCT TTGCTGCAAT TCATAGGTCA 
AAAAGGAAGG TCCTGGGCCG TGGCGATTCT GAAGATGAAC GCTGTCGTAA CCTCCTCCCT 
TCCCCACCTG TCACTCCAAC TGGCTCCTGC CCCAGCTTGC CACGTCAGAC CAGCTCCATC 
CAGCGGAACC TACGTCGCTC CACCACAAGC AATGACAGTT TTAAAGCTTT GCTACTCAAG 
AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC ATGTCAGCCG CTGAGATTCT CAAATGCACA 
GACCCCCGTT TCCAAAGGGC TAATTCAGAG AATGCACTAG CTGACGGACT GTCCTCTCCG 
ACAAGCAACA GGAGAGCACA TGAAGAGTGG GCACGGATGG AGGGAGCTTT GCCTCGCTTT 
ACCACTGGAC AAACTATCCC AAAATATGGG CGCTCGCGCA CACCACCTTC CGCAGCCAGC 
AGCCGGTATA ACAGCCGCAG CCGGATTACC AGTAGGCCAA TGACTGCCAT CTGTGAACGA 
GATGGAGAGG TGGCTGAATC TACAGACTGC TACATAAGTA GCGAGAATCA TTTTGTCTTG 
GCTTTATCCT CCAGCAGCAT GGTCTGCGCT CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291425.2
CDS	347..5071
Translation	MWGERSFLRYRGEWAEGKSHPVPRNIAAMGLSSLTEQRGRFFPCHKDDTDDDYDEWVYAPLHRRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_009293150.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293150.3

ORF Insert Sequence:

ATGTGGGGAG AGAGGAGTTT TCTGAGGTAC AGGGGGGAGT GGGCAGAGGG TAAGAGTCAC 
CCTGTACCTC GAAACATTGC TGCCATGGGT CTTTCTTCAT TGACAGAGCA GCGTGGACGC 
TTCTTTCCCT GCCACAAAGA TGACACTGAT GACGATTATG ATGAGTGGGT TTATGCACCT 
CTCCATCGCA GAGCGGTGTC CAGTCTTGAT GAGGAGAGCA AATGGTCGGT GCACTACACA 
GCCCCCTGGC ACCACCAAGA GAACGTCTTC CTGCCTGGGT CCAGACCAGC ATGTGTTGAG 
GATCTTCACC GTCAGGCCAA AGTCAACCTG AAAACAGTCC TCAGAGAGTG CGACAAGCTC 
AGAAAAGATG GCTTTCGCAG TTCTCAGTAT TACTCCCAAA GCCCAGCATT CTCTGCAACC 
AGCCTTTGTG AAAGTGTACA TCTGGATGAA GAGAAGACAG AAAAGAAAAA GAAGCGGTTT 
AAAGTCCTAG ACTGCCTATC ACAGTCTTGC CTCTCTCTCT GCTGTCATTT GAGATCTTGG 
AAAACAAAGG CTTCCGATTC ACCTAATGAA GAGGATAAAC TTGTGTACTC AATGCCGCCA 
CAAACCCCTC TGCTGGAGCA TGGCTCTGAC ATGGACATCC AGAGAAGCTG GACCAGCTGT 
CTACCTTTGC CCACTCCCGA GGAGAAGATG AGACTGCAGG CCCAATCGGT GGCTACTGAT 
ATAATTCCTA TCAACATTAC AGGTGAGAAC TTTGACCGCC AGGCCAGCTT TCGCCGCTCC 
ATGGCAAACA CTGATACTAT TATACGAAGA CCTAAGAGAG TTCAAAGAAG AAAGACAATA 
ACAGGAGTGC CTGACAACAT CCAGACTGAA CTAGCTAATA AAGAACAAGC TGACTCCAGA 
ATACATTCGA GTATGGCAGA TCCGTACTCC ACCCTCAGTC GAGTGGGAAG TGTAAACTCC 
ACCCTGAAAC GCTCAGACAC CCGTGATTCC TGTTGTCAAA CAGAAGAGGT GAAGATTGTT 
CCTCCCTCAG TACGCAGAAT CAGAGCACAA AGAGGCCATG GAATAGCTGC CCAAATGGCC 
AGTGTCTCCT CCTCAAGCAG CATTTCCACC ATAAGTGACT GTAATGGCAT TAGCTACACA 
CAGCTTAATG ACAGTGATCA GAGCTTTCAA AGTTTACCAA GACAAGGTTC TCGCATCCCG 
CAACAACACA GTGAAACAAA GTACAATAGC TCCCCCTACA GGGCGAATTC TGGTTCTACA 
AGCCCCTTGT CCTACCATTT TAACATGCAG CATGACTCTT CATCATCGCT GCATATGCTG 
TCTGACTCGA GAAGCAAGCT TACTGAAAAT CTAAATTACG AAGGCTCTGA TAAAAATAAT 
ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT CTACAGAACT CCATTCCTTA TGGATACAAA 
AGGACAGAGG ACAACCAAAT GCAATCACCT CATTCGTCAG TCCAGTTGCT CAATTCTGAG 
GATGCCTCAT TGTCGGGTGT CTCCTCGTGT TATGAATCCA CAGCGTCCCT CTGTACAGGT 
ATTCATACAG AGACTGAGTC CCAGTGCAGC ACACTTGATG GCAGAAACTG CTGCAGTCCT 
AACTGTGTCC GCAGGGATTC CAACTTCTCA GAGAGCAGCA ACCAAAGCTG CAGCACACTA 
ACCACGGACC AATGGACGTA TGAAGTTTCT CCAAAAGACA ATGTTACTTC AAGTTGCTCC 
TCACCTGTCA GCCATATATA CCACGCAGAG GAACATTCTC CCAGCAAGAC AGATACAAGC 
TCATTGTTCT CTGTTGACAA TGAAGGTTAC TACACCTCCA TGCGCCTAGA CTCTGGTTTG 
AAGTCACACA GTCACGGCTG CATCAATAAA GCAGGAGAAC AAAAGCACAG TTTGTATGAG 
TGCAAAGACC ACCACAGTCA AAGTGATACT GCAAGTCTGC ATAGCAACCG CTCCCTGACT 
CGTAGCATCT CTCTGCGAAA AGCCAGGAAG CCCCCACTCC CTCCAGAAAG AACCGACTCT 
TTAAGGCGTA AACCCCAGAG GAAGAGTCGC CACAGTGGTT CAGTCCTTAA TGAGCAGTTA 
ATCTCTAGTC TCCAGCAGTC CTTACAACTT AACCTAAATA CTCAAACTAC ATCTTGCTCT 
GAACAGTCAC CCTGTGGTAG GTTTGAAGAC CCTTGGGTCC TCCGACCGAG AAGTCAGAGT 
ATCATAAGTG CAGCTAGCAG TGGGGTATCT GCACCAGCTG CTGTATGTCC GGTCACACCA 
ACACACAGTG ACAGCAGCAG TCAGCGCTCA GACTATGCAG AGTCATGGGA CTTCTACATG 
GATTGCCCAT GTCCACGGTC TGACCAGAGT CTTTCCTCAC AAATCACAAG GTCTGCAAGT 
GCTGAGGAGA ATCATGGAGT CATAGGTAAT GCATCCTACA ACAGTAAATT CCCATCATCC 
CCCTTCAGCA ATAGTCAGTC AAAATTGACC TCATCTCCTG ACAAAGTCCA TCGACTCACG 
TCACCATCAA GTGGCTACTC AAGTCAGTCT AACACTCCAA CTGCAGGCAC TCCAGTTACG 
TCTCTAATAA GAGCAAAGTC TCCTGCGGGG AGACCGAAAC CAAAAGTGCC AGAAAGAAAG 
TCCTCTCTAC GATCTTCTAT ATCATCCTCT TCTACATCCC TGTCCTCCAA CACCTCAGAT 
TCCATCAGGA ACATTCCTCC ACCTCCACCT TTACCTGACA TGACAACTGC ATTATGTGGG 
TCGCCCAGTT CTGCTTCTTT CTCTCCAACA GCGGCTAAAA TAGACCCAAT ACCCTCTTTT 
ACATCTCTAC AGGATGTATC ATCTGCCTTG GAAGATGGGC AAAATACACT TTCAGATACA 
TCTGCAACAC CTGAGGAATG TGATAGTCCT GATTTTCCCC CTCCCCATTC AAATGTTTTT 
GAATCTCTGT CAGGCAATGT AGATACCAAC AGCCTCCCAC CTCCTCCACT TCTGCTTTGC 
AAACCTTCAC TGTGCATTTC ATCTCTTCTT CCCCCTCCTC CTCCACCTCC TCCACTATTA 
CTTGAGACCA GTCTTCAACC AGCCACCATT TTGAAGACAG AGAGCAGTCT TACACCTACA 
ACAGCTGTAA AATGTGAAAA AGAAGTAAAC ATCAGTCACA CTGGAGAGCA AGAAAAGACT 
CAGAGACCAA TAATCACTGC CCAGGCCCTT CAGATGGTGC AGTTGCGACC TGTTAAGCTC 
AAGCAAATAG AAACTATTTT TACATCTGTC AGTTTTGATC ATTCTGATCA CCAAGCACAA 
AACCATTCTG ATACAAACTC AGGAAAGTCC CTGGAAATTG ATAATACAGG GCAAACAGTG 
ACCGTCAATT GTGTTACTCA GTCAGAAAAT AGTATGGAAA CATCTGAATG TGAATATTCG 
GTGAACAGAT TCATAGACGA TCTCCCTGAG GTATCAACTA GCACTCTACC CCCTGACCTA 
GATGAACAAT TAAAAAAAAC TGAGCAAAAT AATCCAGTGC ACCTCTCTTC CAAGTCCACA 
AATTCCTTAG CTTCTCCTCT GAGTTCTCCA ATGAAACAGA AGCCTCCTAT ATCACCCAAA 
AAACCCAATC TTTCCCTTAT TATACCACCC ATAATGCACT TGCCATTTTC CAACGGACTC 
AAGCTTCAGC AGGTTCATGA AGCACAAGAG ACACTAGAGT CAGAGCAAGT GAATGGTGCA 
TCCTCGACAA ACACAGTTAA TTTGCAAGCA ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG 
AGTGACTCAG ACTTATCAAC CCCTGTGGTC AAGAGCAGAC TATCTTTCTG CAGCGAGTTA 
TCATCAAGCA GTTTCCAAGA CCTGCAACTT TCTGATTACA TTCTTAATGA GCATGACCTC 
GGCCTGAGCG ATAAAGACTT TGGGCTCTGT GATGATAAAA GCACATCAGA TGATGGTTCA 
AGCAGCAGCT CAGGATCCAT CAGCTTTAAA GAGGATGAAC ATGAGGAAAA CGGTGCTGTG 
TTTGACTCAA TCACTGAAAG CACATCTGAC ATTAACGCTA GCGGAGAAGC TGTGGAGGGG 
ATGGTGACGC CTGTTCGCCC TCGTACTACA GAGGACCTCT TTGCTGCAAT TCATAGGTCA 
AAAAGGAAGG TCCTGGGCCG TGGCGATTCT GAAGATGAAC GCTGTCGTAA CCTCCTCCCT 
TCCCCACCTG TCACTCCAAC TGGCTCCTGC CCCAGCTTGC CACGTCAGAC CAGCTCCATC 
CAGCGGAACC TACGTCGCTC CACCACAAGC AATGACAGTT TTAAAGCTTT GCTACTCAAG 
AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC ATGTCAGCCG CTGAGATTCT CAAATGCACA 
GACCCCCGTT TCCAAAGGGC TAATTCAGAG AATGCACTAG CTGACGGACT GTCCTCTCCG 
ACAAGCAACA GGAGAGCACA TGAAGAGTGG GCACGGATGG AGGGAGCTTT GCCTCGCTTT 
ACCACTGGAC AAACTATCCC AAAATATGGG CGCTCGCGCA CACCACCTTC CGCAGCCAGC 
AGCCGGTATA ACAGCCGCAG CCGGATTACC AGTAGGCCAA TGACTGCCAT CTGTGAACGA 
GATGGAGAGG TGGCTGAATC TACAGACTGC TACATAAGTA GCGAGAATCA TTTTGTCTTG 
GCTTTATCCT CCAGCAGCAT GGTCTGCGCT CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158822.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158879.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.

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CloneID	ODa145948
Clone ID Related Accession (Same CDS sequence)	XM_005158822.4
Accession Version	XM_005158822.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158822.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGT GTGGGACGAA GTTGGGGGGG GCAGTTGCTC CGGCTGGGGC ACTGGGCTGT 
GTGAGGACAG TGCGCTGCAA AACTCTTTTG ACACACTTCC CAGCAAATAC CAACACACAG 
CGGGAGAGGC AGGAGACCAT GTTCTGTCTG AGAGCGGTGT CCAGTCTTGA TGAGGAGAGC 
AAATGGTCGG TGCACTACAC AGCCCCCTGG CACCACCAAG AGAACGTCTT CCTGCCTGGG 
TCCAGACCAG CATGTGTTGA GGATCTTCAC CGTCAGGCCA AAGTCAACCT GAAAACAGTC 
CTCAGAGAGT GCGACAAGCT CAGAAAAGAT GGCTTTCGCA GTTCTCAGTA TTACTCCCAA 
AGCCCAGCAT TCTCTGCAAC CAGCCTTTGT GAAAGTGTAC ATCTGGATGA AGAGAAGACA 
GAAAAGAAAA AGAAGCGGTT TAAAGTCCTA GACTGCCTAT CACAGTCTTG CCTCTCTCTC 
TGCTGTCATT TGAGATCTTG GAAAACAAAG GCTTCCGATT CACCTAATGA AGAGGATAAA 
CTTGTGTACT CAATGCCGCC ACAAACCCCT CTGCTGGAGC ATGGCTCTGA CATGGACATC 
CAGAGAAGCT GGACCAGCTG TCTACCTTTG CCCACTCCCG AGGAGAAGAT GAGACTGCAG 
GCCCAATCGG TGGCTACTGA TATAATTCCT ATCAACATTA CAGGTGAGAA CTTTGACCGC 
CAGGCCAGCT TTCGCCGCTC CATGGCAAAC ACTGATACTA TTATACGAAG ACCTAAGAGA 
GTTCAAAGAA GAAAGACAAT AACAGGAGTG CCTGACAACA TCCAGACTGA ACTAGCTAAT 
AAAGAACAAG CTGACTCCAG AATACATTCG AGTATGGCAG ATCCGTACTC CACCCTCAGT 
CGAGTGGGAA GTGTAAACTC CACCCTGAAA CGCTCAGACA CCCGTGATTC CTGTTGTCAA 
ACAGAAGAGG TGAAGATTGT TCCTCCCTCA GTACGCAGAA TCAGAGCACA AAGAGGCCAT 
GGAATAGCTG CCCAAATGGC CAGTGTCTCC TCCTCAAGCA GCATTTCCAC CATAAGTGAC 
TGTAATGGCA TTAGCTACAC ACAGCTTAAT GACAGTGATC AGAGCTTTCA AAGTTTACCA 
AGACAAGGTT CTCGCATCCC GCAACAACAC AGTGAAACAA AGTACAATAG CTCCCCCTAC 
AGGGCGAATT CTGGTTCTAC AAGCCCCTTG TCCTACCATT TTAACATGCA GCATGACTCT 
TCATCATCGC TGCATATGCT GTCTGACTCG AGAAGCAAGC TTACTGAAAA TCTAAATTAC 
GAAGGCTCTG ATAAAAATAA TACCTCAAGC TTGTTTAGTC TCAGTGGTGC TCTACAGAAC 
TCCATTCCTT ATGGATACAA AAGGACAGAG GACAACCAAA TGCAATCACC TCATTCGTCA 
GTCCAGTTGC TCAATTCTGA GGATGCCTCA TTGTCGGGTG TCTCCTCGTG TTATGAATCC 
ACAGCGTCCC TCTGTACAGG TATTCATACA GAGACTGAGT CCCAGTGCAG CACACTTGAT 
GGCAGAAACT GCTGCAGTCC TAACTGTGTC CGCAGGGATT CCAACTTCTC AGAGAGCAGC 
AACCAAAGCT GCAGCACACT AACCACGGAC CAATGGACGT ATGAAGTTTC TCCAAAAGAC 
AATGTTACTT CAAGTTGCTC CTCACCTGTC AGCCATATAT ACCACGCAGA GGAACATTCT 
CCCAGCAAGA CAGATACAAG CTCATTGTTC TCTGTTGACA ATGAAGGTTA CTACACCTCC 
ATGCGCCTAG ACTCTGGTTT GAAGTCACAC AGTCACGGCT GCATCAATAA AGCAGGAGAA 
CAAAAGCACA GTTTGTATGA GTGCAAAGAC CACCACAGTC AAAGTGATAC TGCAAGTCTG 
CATAGCAACC GCTCCCTGAC TCGTAGCATC TCTCTGCGAA AAGCCAGGAA GCCCCCACTC 
CCTCCAGAAA GAACCGACTC TTTAAGGCGT AAACCCCAGA GGAAGAGTCG CCACAGTGGT 
TCAGTCCTTA ATGAGCAGTT AATCTCTAGT CTCCAGCAGT CCTTACAACT TAACCTAAAT 
ACTCAAACTA CATCTTGCTC TGAACAGTCA CCCTGTGGTA GGTTTGAAGA CCCTTGGGTC 
CTCCGACCGA GAAGTCAGAG TATCATAAGT GCAGCTAGCA GTGGGGTATC TGCACCAGCT 
GCTGTATGTC CGGTCACACC AACACACAGT GACAGCAGCA GTCAGCGCTC AGACTATGCA 
GAGTCATGGG ACTTCTACAT GGATTGCCCA TGTCCACGGT CTGACCAGAG TCTTTCCTCA 
CAAATCACAA GGTCTGCAAG TGCTGAGGAG AATCATGGAG TCATAGGTAA TGCATCCTAC 
AACAGTAAAT TCCCATCATC CCCCTTCAGC AATAGTCAGT CAAAATTGAC CTCATCTCCT 
GACAAAGTCC ATCGACTCAC GTCACCATCA AGTGGCTACT CAAGTCAGTC TAACACTCCA 
ACTGCAGGCA CTCCAGTTAC GTCTCTAATA AGAGCAAAGT CTCCTGCGGG GAGACCGAAA 
CCAAAAGTGC CAGAAAGAAA GTCCTCTCTA CGATCTTCTA TATCATCCTC TTCTACATCC 
CTGTCCTCCA ACACCTCAGA TTCCATCAGG AACATTCCTC CACCTCCACC TTTACCTGAC 
ATGACAACTG CATTATGTGG GTCGCCCAGT TCTGCTTCTT TCTCTCCAAC AGCGGCTAAA 
ATAGACCCAA TACCCTCTTT TACATCTCTA CAGGATGTAT CATCTGCCTT GGAAGATGGG 
CAAAATACAC TTTCAGATAC ATCTGCAACA CCTGAGGAAT GTGATAGTCC TGATTTTCCC 
CCTCCCCATT CAAATGTTTT TGAATCTCTG TCAGGCAATG TAGATACCAA CAGCCTCCCA 
CCTCCTCCAC TTCTGCTTTG CAAACCTTCA CTGTGCATTT CATCTCTTCT TCCCCCTCCT 
CCTCCACCTC CTCCACTATT ACTTGAGACC AGTCTTCAAC CAGCCACCAT TTTGAAGACA 
GAGAGCAGTC TTACACCTAC AACAGCTGTA AAATGTGAAA AAGAAGTAAA CATCAGTCAC 
ACTGGAGAGC AAGAAAAGAC TCAGAGACCA ATAATCACTG CCCAGGCCCT TCAGATGGTG 
CAGTTGCGAC CTGTTAAGCT CAAGCAAATA GAAACTATTT TTACATCTGT CAGTTTTGAT 
CATTCTGATC ACCAAGCACA AAACCATTCT GATACAAACT CAGGAAAGTC CCTGGAAATT 
GATAATACAG GGCAAACAGT GACCGTCAAT TGTGTTACTC AGTCAGAAAA TAGTATGGAA 
ACATCTGAAT GTGAATATTC GGTGAACAGA TTCATAGACG ATCTCCCTGA GGTATCAACT 
AGCACTCTAC CCCCTGACCT AGATGAACAA TTAAAAAAAA CTGAGCAAAA TAATCCAGTG 
CACCTCTCTT CCAAGTCCAC AAATTCCTTA GCTTCTCCTC TGAGTTCTCC AATGAAACAG 
AAGCCTCCTA TATCACCCAA AAAACCCAAT CTTTCCCTTA TTATACCACC CATAATGCAC 
TTGCCATTTT CCAACGGACT CAAGCTTCAG CAGGTTCATG AAGCACAAGA GACACTAGAG 
TCAGAGCAAG TGAATGGTGC ATCCTCGACA AACACAGTTA ATTTGCAAGC AATTCCTTTA 
GACCTTGAGG TAGAGGAAGA GAGTGACTCA GACTTATCAA CCCCTGTGGT CAAGAGCAGA 
CTATCTTTCT GCAGCGAGTT ATCATCAAGC AGTTTCCAAG ACCTGCAACT TTCTGATTAC 
ATTCTTAATG AGCATGACCT CGGCCTGAGC GATAAAGACT TTGGGCTCTG TGATGATAAA 
AGCACATCAG ATGATGGTTC AAGCAGCAGC TCAGGATCCA TCAGCTTTAA AGAGGATGAA 
CATGAGGAAA ACGGTGCTGT GTTTGACTCA ATCACTGAAA GCACATCTGA CATTAACGCT 
AGCGGAGAAG CTGTGGAGGG GATGGTGACG CCTGTTCGCC CTCGTACTAC AGAGGACCTC 
TTTGCTGCAA TTCATAGGTC AAAAAGGAAG GTCCTGGGCC GTGGCGATTC TGAAGATGAA 
CGCTGTCGTA ACCTCCTCCC TTCCCCACCT GTCACTCCAA CTGGCTCCTG CCCCAGCTTG 
CCACGTCAGA CCAGCTCCAT CCAGCGGAAC CTACGTCGCT CCACCACAAG CAATGACAGT 
TTTAAAGCTT TGCTACTCAA GAAGGGCAGC CGCTCAGAGT CCAGCTTTCG CATGTCAGCC 
GCTGAGATTC TCAAATGCAC AGACCCCCGT TTCCAAAGGG CTAATTCAGA GAATGCACTA 
GCTGACGGAC TGTCCTCTCC GACAAGCAAC AGGAGAGCAC ATGAAGAGTG GGCACGGATG 
GAGGGAGCTT TGCCTCGCTT TACCACTGGA CAAACTATCC CAAAATATGG GCGCTCGCGC 
ACACCACCTT CCGCAGCCAG CAGCCGGTAT AACAGCCGCA GCCGGATTAC CAGTAGGCCA 
ATGACTGCCA TCTGTGAACG AGATGGAGAG GTGGCTGAAT CTACAGACTG CTACATAAGT 
AGCGAGAATC ATTTTGTCTT GGCTTTATCC TCCAGCAGCA TGGTCTGCGC TCAAGGCAGC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158879.2
CDS	164..4849
Translation	MNLCGTKLGGAVAPAGALGCVRTVRCKTLLTHFPANTNTQRERQETMFCLRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158822.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158822.4

ORF Insert Sequence:

ATGAACCTGT GTGGGACGAA GTTGGGGGGG GCAGTTGCTC CGGCTGGGGC ACTGGGCTGT 
GTGAGGACAG TGCGCTGCAA AACTCTTTTG ACACACTTCC CAGCAAATAC CAACACACAG 
CGGGAGAGGC AGGAGACCAT GTTCTGTCTG AGAGCGGTGT CCAGTCTTGA TGAGGAGAGC 
AAATGGTCGG TGCACTACAC AGCCCCCTGG CACCACCAAG AGAACGTCTT CCTGCCTGGG 
TCCAGACCAG CATGTGTTGA GGATCTTCAC CGTCAGGCCA AAGTCAACCT GAAAACAGTC 
CTCAGAGAGT GCGACAAGCT CAGAAAAGAT GGCTTTCGCA GTTCTCAGTA TTACTCCCAA 
AGCCCAGCAT TCTCTGCAAC CAGCCTTTGT GAAAGTGTAC ATCTGGATGA AGAGAAGACA 
GAAAAGAAAA AGAAGCGGTT TAAAGTCCTA GACTGCCTAT CACAGTCTTG CCTCTCTCTC 
TGCTGTCATT TGAGATCTTG GAAAACAAAG GCTTCCGATT CACCTAATGA AGAGGATAAA 
CTTGTGTACT CAATGCCGCC ACAAACCCCT CTGCTGGAGC ATGGCTCTGA CATGGACATC 
CAGAGAAGCT GGACCAGCTG TCTACCTTTG CCCACTCCCG AGGAGAAGAT GAGACTGCAG 
GCCCAATCGG TGGCTACTGA TATAATTCCT ATCAACATTA CAGGTGAGAA CTTTGACCGC 
CAGGCCAGCT TTCGCCGCTC CATGGCAAAC ACTGATACTA TTATACGAAG ACCTAAGAGA 
GTTCAAAGAA GAAAGACAAT AACAGGAGTG CCTGACAACA TCCAGACTGA ACTAGCTAAT 
AAAGAACAAG CTGACTCCAG AATACATTCG AGTATGGCAG ATCCGTACTC CACCCTCAGT 
CGAGTGGGAA GTGTAAACTC CACCCTGAAA CGCTCAGACA CCCGTGATTC CTGTTGTCAA 
ACAGAAGAGG TGAAGATTGT TCCTCCCTCA GTACGCAGAA TCAGAGCACA AAGAGGCCAT 
GGAATAGCTG CCCAAATGGC CAGTGTCTCC TCCTCAAGCA GCATTTCCAC CATAAGTGAC 
TGTAATGGCA TTAGCTACAC ACAGCTTAAT GACAGTGATC AGAGCTTTCA AAGTTTACCA 
AGACAAGGTT CTCGCATCCC GCAACAACAC AGTGAAACAA AGTACAATAG CTCCCCCTAC 
AGGGCGAATT CTGGTTCTAC AAGCCCCTTG TCCTACCATT TTAACATGCA GCATGACTCT 
TCATCATCGC TGCATATGCT GTCTGACTCG AGAAGCAAGC TTACTGAAAA TCTAAATTAC 
GAAGGCTCTG ATAAAAATAA TACCTCAAGC TTGTTTAGTC TCAGTGGTGC TCTACAGAAC 
TCCATTCCTT ATGGATACAA AAGGACAGAG GACAACCAAA TGCAATCACC TCATTCGTCA 
GTCCAGTTGC TCAATTCTGA GGATGCCTCA TTGTCGGGTG TCTCCTCGTG TTATGAATCC 
ACAGCGTCCC TCTGTACAGG TATTCATACA GAGACTGAGT CCCAGTGCAG CACACTTGAT 
GGCAGAAACT GCTGCAGTCC TAACTGTGTC CGCAGGGATT CCAACTTCTC AGAGAGCAGC 
AACCAAAGCT GCAGCACACT AACCACGGAC CAATGGACGT ATGAAGTTTC TCCAAAAGAC 
AATGTTACTT CAAGTTGCTC CTCACCTGTC AGCCATATAT ACCACGCAGA GGAACATTCT 
CCCAGCAAGA CAGATACAAG CTCATTGTTC TCTGTTGACA ATGAAGGTTA CTACACCTCC 
ATGCGCCTAG ACTCTGGTTT GAAGTCACAC AGTCACGGCT GCATCAATAA AGCAGGAGAA 
CAAAAGCACA GTTTGTATGA GTGCAAAGAC CACCACAGTC AAAGTGATAC TGCAAGTCTG 
CATAGCAACC GCTCCCTGAC TCGTAGCATC TCTCTGCGAA AAGCCAGGAA GCCCCCACTC 
CCTCCAGAAA GAACCGACTC TTTAAGGCGT AAACCCCAGA GGAAGAGTCG CCACAGTGGT 
TCAGTCCTTA ATGAGCAGTT AATCTCTAGT CTCCAGCAGT CCTTACAACT TAACCTAAAT 
ACTCAAACTA CATCTTGCTC TGAACAGTCA CCCTGTGGTA GGTTTGAAGA CCCTTGGGTC 
CTCCGACCGA GAAGTCAGAG TATCATAAGT GCAGCTAGCA GTGGGGTATC TGCACCAGCT 
GCTGTATGTC CGGTCACACC AACACACAGT GACAGCAGCA GTCAGCGCTC AGACTATGCA 
GAGTCATGGG ACTTCTACAT GGATTGCCCA TGTCCACGGT CTGACCAGAG TCTTTCCTCA 
CAAATCACAA GGTCTGCAAG TGCTGAGGAG AATCATGGAG TCATAGGTAA TGCATCCTAC 
AACAGTAAAT TCCCATCATC CCCCTTCAGC AATAGTCAGT CAAAATTGAC CTCATCTCCT 
GACAAAGTCC ATCGACTCAC GTCACCATCA AGTGGCTACT CAAGTCAGTC TAACACTCCA 
ACTGCAGGCA CTCCAGTTAC GTCTCTAATA AGAGCAAAGT CTCCTGCGGG GAGACCGAAA 
CCAAAAGTGC CAGAAAGAAA GTCCTCTCTA CGATCTTCTA TATCATCCTC TTCTACATCC 
CTGTCCTCCA ACACCTCAGA TTCCATCAGG AACATTCCTC CACCTCCACC TTTACCTGAC 
ATGACAACTG CATTATGTGG GTCGCCCAGT TCTGCTTCTT TCTCTCCAAC AGCGGCTAAA 
ATAGACCCAA TACCCTCTTT TACATCTCTA CAGGATGTAT CATCTGCCTT GGAAGATGGG 
CAAAATACAC TTTCAGATAC ATCTGCAACA CCTGAGGAAT GTGATAGTCC TGATTTTCCC 
CCTCCCCATT CAAATGTTTT TGAATCTCTG TCAGGCAATG TAGATACCAA CAGCCTCCCA 
CCTCCTCCAC TTCTGCTTTG CAAACCTTCA CTGTGCATTT CATCTCTTCT TCCCCCTCCT 
CCTCCACCTC CTCCACTATT ACTTGAGACC AGTCTTCAAC CAGCCACCAT TTTGAAGACA 
GAGAGCAGTC TTACACCTAC AACAGCTGTA AAATGTGAAA AAGAAGTAAA CATCAGTCAC 
ACTGGAGAGC AAGAAAAGAC TCAGAGACCA ATAATCACTG CCCAGGCCCT TCAGATGGTG 
CAGTTGCGAC CTGTTAAGCT CAAGCAAATA GAAACTATTT TTACATCTGT CAGTTTTGAT 
CATTCTGATC ACCAAGCACA AAACCATTCT GATACAAACT CAGGAAAGTC CCTGGAAATT 
GATAATACAG GGCAAACAGT GACCGTCAAT TGTGTTACTC AGTCAGAAAA TAGTATGGAA 
ACATCTGAAT GTGAATATTC GGTGAACAGA TTCATAGACG ATCTCCCTGA GGTATCAACT 
AGCACTCTAC CCCCTGACCT AGATGAACAA TTAAAAAAAA CTGAGCAAAA TAATCCAGTG 
CACCTCTCTT CCAAGTCCAC AAATTCCTTA GCTTCTCCTC TGAGTTCTCC AATGAAACAG 
AAGCCTCCTA TATCACCCAA AAAACCCAAT CTTTCCCTTA TTATACCACC CATAATGCAC 
TTGCCATTTT CCAACGGACT CAAGCTTCAG CAGGTTCATG AAGCACAAGA GACACTAGAG 
TCAGAGCAAG TGAATGGTGC ATCCTCGACA AACACAGTTA ATTTGCAAGC AATTCCTTTA 
GACCTTGAGG TAGAGGAAGA GAGTGACTCA GACTTATCAA CCCCTGTGGT CAAGAGCAGA 
CTATCTTTCT GCAGCGAGTT ATCATCAAGC AGTTTCCAAG ACCTGCAACT TTCTGATTAC 
ATTCTTAATG AGCATGACCT CGGCCTGAGC GATAAAGACT TTGGGCTCTG TGATGATAAA 
AGCACATCAG ATGATGGTTC AAGCAGCAGC TCAGGATCCA TCAGCTTTAA AGAGGATGAA 
CATGAGGAAA ACGGTGCTGT GTTTGACTCA ATCACTGAAA GCACATCTGA CATTAACGCT 
AGCGGAGAAG CTGTGGAGGG GATGGTGACG CCTGTTCGCC CTCGTACTAC AGAGGACCTC 
TTTGCTGCAA TTCATAGGTC AAAAAGGAAG GTCCTGGGCC GTGGCGATTC TGAAGATGAA 
CGCTGTCGTA ACCTCCTCCC TTCCCCACCT GTCACTCCAA CTGGCTCCTG CCCCAGCTTG 
CCACGTCAGA CCAGCTCCAT CCAGCGGAAC CTACGTCGCT CCACCACAAG CAATGACAGT 
TTTAAAGCTT TGCTACTCAA GAAGGGCAGC CGCTCAGAGT CCAGCTTTCG CATGTCAGCC 
GCTGAGATTC TCAAATGCAC AGACCCCCGT TTCCAAAGGG CTAATTCAGA GAATGCACTA 
GCTGACGGAC TGTCCTCTCC GACAAGCAAC AGGAGAGCAC ATGAAGAGTG GGCACGGATG 
GAGGGAGCTT TGCCTCGCTT TACCACTGGA CAAACTATCC CAAAATATGG GCGCTCGCGC 
ACACCACCTT CCGCAGCCAG CAGCCGGTAT AACAGCCGCA GCCGGATTAC CAGTAGGCCA 
ATGACTGCCA TCTGTGAACG AGATGGAGAG GTGGCTGAAT CTACAGACTG CTACATAAGT 
AGCGAGAATC ATTTTGTCTT GGCTTTATCC TCCAGCAGCA TGGTCTGCGC TCAAGGCAGC 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158823.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158880.2 Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.

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CloneID	ODa145949
Clone ID Related Accession (Same CDS sequence)	XM_005158823.4
Accession Version	XM_005158823.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158823.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGCGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AGAAAAGAAA AAGAAGCGGT TTAAAGTCCT AGACTGCCTA 
TCACAGTCTT GCCTCTCTCT CTGCTGTCAT TTGAGATCTT GGAAAACAAA GGCTTCCGAT 
TCACCTAATG AAGAGGATAA ACTTGTGTAC TCAATGCCGC CACAAACCCC TCTGCTGGAG 
CATGGCTCTG ACATGGACAT CCAGAGAAGC TGGACCAGCT GTCTACCTTT GCCCACTCCC 
GAGGAGAAGA TGAGACTGCA GGCCCAATCG GTGGCTACTG ATATAATTCC TATCAACATT 
ACAGGTGAGA ACTTTGACCG CCAGGCCAGC TTTCGCCGCT CCATGGCAAA CACTGATACT 
ATTATACGAA GACCTAAGAG AGTTCAAAGA AGAAAGACAA TAACAGGAGT GCCTGACAAC 
ATCCAGACTG AACTAGCTAA TAAAGAACAA GCTGACTCCA GAATACATTC GAGTATGGCA 
GATCCGTACT CCACCCTCAG TCGAGTGGGA AGTGTAAACT CCACCCTGAA ACGCTCAGAC 
ACCCGTGATT CCTGTTGTCA AACAGAAGAG GTGAAGATTG TTCCTCCCTC AGTACGCAGA 
ATCAGAGCAC AAAGAGGCCA TGGAATAGCT GCCCAAATGG CCAGTGTCTC CTCCTCAAGC 
AGCATTTCCA CCATAAGTGA CTGTAATGGC ATTAGCTACA CACAGCTTAA TGACAGTGAT 
CAGAGCTTTC AAAGTTTACC AAGACAAGGT TCTCGCATCC CGCAACAACA CAGTGAAACA 
AAGTACAATA GCTCCCCCTA CAGGGCGAAT TCTGGTTCTA CAAGCCCCTT GTCCTACCAT 
TTTAACATGC AGCATGACTC TTCATCATCG CTGCATATGC TGTCTGACTC GAGAAGCAAG 
CTTACTGAAA ATCTAAATTA CGAAGGCTCT GATAAAAATA ATACCTCAAG CTTGTTTAGT 
CTCAGTGGTG CTCTACAGAA CTCCATTCCT TATGGATACA AAAGGACAGA GGACAACCAA 
ATGCAATCAC CTCATTCGTC AGTCCAGTTG CTCAATTCTG AGGATGCCTC ATTGTCGGGT 
GTCTCCTCGT GTTATGAATC CACAGCGTCC CTCTGTACAG GTATTCATAC AGAGACTGAG 
TCCCAGTGCA GCACACTTGA TGGCAGAAAC TGCTGCAGTC CTAACTGTGT CCGCAGGGAT 
TCCAACTTCT CAGAGAGCAG CAACCAAAGC TGCAGCACAC TAACCACGGA CCAATGGACG 
TATGAAGTTT CTCCAAAAGA CAATGTTACT TCAAGTTGCT CCTCACCTGT CAGCCATATA 
TACCACGCAG AGGAACATTC TCCCAGCAAG ACAGATACAA GCTCATTGTT CTCTGTTGAC 
AATGAAGGTT ACTACACCTC CATGCGCCTA GACTCTGGTT TGAAGTCACA CAGTCACGGC 
TGCATCAATA AAGCAGGAGA ACAAAAGCAC AGTTTGTATG AGTGCAAAGA CCACCACAGT 
CAAAGTGATA CTGCAAGTCT GCATAGCAAC CGCTCCCTGA CTCGTAGCAT CTCTCTGCGA 
AAAGCCAGGA AGCCCCCACT CCCTCCAGAA AGAACCGACT CTTTAAGGCG TAAACCCCAG 
AGGAAGAGTC GCCACAGTGG TTCAGTCCTT AATGAGCAGT TAATCTCTAG TCTCCAGCAG 
TCCTTACAAC TTAACCTAAA TACTCAAACT ACATCTTGCT CTGAACAGTC ACCCTGTGGT 
AGGTTTGAAG ACCCTTGGGT CCTCCGACCG AGAAGTCAGA GTATCATAAG TGCAGCTAGC 
AGTGGGGTAT CTGCACCAGC TGCTGTATGT CCGGTCACAC CAACACACAG TGACAGCAGC 
AGTCAGCGCT CAGACTATGC AGAGTCATGG GACTTCTACA TGGATTGCCC ATGTCCACGG 
TCTGACCAGA GTCTTTCCTC ACAAATCACA AGGTCTGCAA GTGCTGAGGA GAATCATGGA 
GTCATAGGTA ATGCATCCTA CAACAGTAAA TTCCCATCAT CCCCCTTCAG CAATAGTCAG 
TCAAAATTGA CCTCATCTCC TGACAAAGTC CATCGACTCA CGTCACCATC AAGTGGCTAC 
TCAAGTCAGT CTAACACTCC AACTGCAGGC ACTCCAGTTA CGTCTCTAAT AAGAGCAAAG 
TCTCCTGCGG GGAGACCGAA ACCAAAAGTG CCAGAAAGAA AGTCCTCTCT ACGATCTTCT 
ATATCATCCT CTTCTACATC CCTGTCCTCC AACACCTCAG ATTCCATCAG GAACATTCCT 
CCACCTCCAC CTTTACCTGA CATGACAACT GCATTATGTG GGTCGCCCAG TTCTGCTTCT 
TTCTCTCCAA CAGCGGCTAA AATAGACCCA ATACCCTCTT TTACATCTCT ACAGGATGTA 
TCATCTGCCT TGGAAGATGG GCAAAATACA CTTTCAGATA CATCTGCAAC ACCTGAGGAA 
TGTGATAGTC CTGATTTTCC CCCTCCCCAT TCAAATGTTT TTGAATCTCT GTCAGGCAAT 
GTAGATACCA ACAGCCTCCC ACCTCCTCCA CTTCTGCTTT GCAAACCTTC ACTGTGCATT 
TCATCTCTTC TTCCCCCTCC TCCTCCACCT CCTCCACTAT TACTTGAGAC CAGTCTTCAA 
CCAGCCACCA TTTTGAAGAC AGAGAGCAGT CTTACACCTA CAACAGCTGT AAAATGTGAA 
AAAGAAGTAA ACATCAGTCA CACTGGAGAG CAAGAAAAGA CTCAGAGACC AATAATCACT 
GCCCAGGCCC TTCAGATGGT GCAGTTGCGA CCTGTTAAGC TCAAGCAAAT AGAAACTATT 
TTTACATCTG TCAGTTTTGA TCATTCTGAT CACCAAGCAC AAAACCATTC TGATACAAAC 
TCAGGAAAGT CCCTGGAAAT TGATAATACA GGGCAAACAG TGACCGTCAA TTGTGTTACT 
CAGTCAGAAA ATAGTATGGA AACATCTGAA TGTGAATATT CGGTGAACAG ATTCATAGAC 
GATCTCCCTG AGGTATCAAC TAGCACTCTA CCCCCTGACC TAGATGAACA ATTAAAAAAA 
ACTGAGCAAA ATAATCCAGT GCACCTCTCT TCCAAGTCCA CAAATTCCTT AGCTTCTCCT 
CTGAGTTCTC CAATGAAACA GAAGCCTCCT ATATCACCCA AAAAACCCAA TCTTTCCCTT 
ATTATACCAC CCATAATGCA CTTGCCATTT TCCAACGGAC TCAAGCTTCA GCAGGTTCAT 
GAAGCACAAG AGACACTAGA GTCAGAGCAA GTGAATGGTG CATCCTCGAC AAACACAGTT 
AATTTGCAAG CAATTCCTTT AGACCTTGAG GTAGAGGAAG AGAGTGACTC AGACTTATCA 
ACCCCTGTGG TCAAGAGCAG ACTATCTTTC TGCAGCGAGT TATCATCAAG CAGTTTCCAA 
GACCTGCAAC TTTCTGATTA CATTCTTAAT GAGCATGACC TCGGCCTGAG CGATAAAGAC 
TTTGGGCTCT GTGATGATAA AAGCACATCA GATGATGGTT CAAGCAGCAG CTCAGGATCC 
ATCAGCTTTA AAGAGGATGA ACATGAGGAA AACGGTGCTG TGTTTGACTC AATCACTGAA 
AGCACATCTG ACATTAACGC TAGCGGAGAA GCTGTGGAGG GGATGGTGAC GCCTGTTCGC 
CCTCGTACTA CAGAGGACCT CTTTGCTGCA ATTCATAGGT CAAAAAGGAA GGTCCTGGGC 
CGTGGCGATT CTGAAGATGA ACGCTGTCGT AACCTCCTCC CTTCCCCACC TGTCACTCCA 
ACTGGCTCCT GCCCCAGCTT GCCACGTCAG ACCAGCTCCA TCCAGCGGAA CCTACGTCGC 
TCCACCACAA GCAATGACAG TTTTAAAGCT TTGCTACTCA AGAAGGGCAG CCGCTCAGAG 
TCCAGCTTTC GCATGTCAGC CGCTGAGATT CTCAAATGCA CAGACCCCCG TTTCCAAAGG 
GCTAATTCAG AGAATGCACT AGCTGACGGA CTGTCCTCTC CGACAAGCAA CAGGAGAGCA 
CATGAAGAGT GGGCACGGAT GGAGGGAGCT TTGCCTCGCT TTACCACTGG ACAAACTATC 
CCAAAATATG GGCGCTCGCG CACACCACCT TCCGCAGCCA GCAGCCGGTA TAACAGCCGC 
AGCCGGATTA CCAGTAGGCC AATGACTGCC ATCTGTGAAC GAGATGGAGA GGTGGCTGAA 
TCTACAGACT GCTACATAAG TAGCGAGAAT CATTTTGTCT TGGCTTTATC CTCCAGCAGC 
ATGGTCTGCG CTCAAGGCAG CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158880.2
CDS	180..4766
Translation	MVFIGTTLKSVIKYFKKKAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158823.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158823.4

ORF Insert Sequence:

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGCGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AGAAAAGAAA AAGAAGCGGT TTAAAGTCCT AGACTGCCTA 
TCACAGTCTT GCCTCTCTCT CTGCTGTCAT TTGAGATCTT GGAAAACAAA GGCTTCCGAT 
TCACCTAATG AAGAGGATAA ACTTGTGTAC TCAATGCCGC CACAAACCCC TCTGCTGGAG 
CATGGCTCTG ACATGGACAT CCAGAGAAGC TGGACCAGCT GTCTACCTTT GCCCACTCCC 
GAGGAGAAGA TGAGACTGCA GGCCCAATCG GTGGCTACTG ATATAATTCC TATCAACATT 
ACAGGTGAGA ACTTTGACCG CCAGGCCAGC TTTCGCCGCT CCATGGCAAA CACTGATACT 
ATTATACGAA GACCTAAGAG AGTTCAAAGA AGAAAGACAA TAACAGGAGT GCCTGACAAC 
ATCCAGACTG AACTAGCTAA TAAAGAACAA GCTGACTCCA GAATACATTC GAGTATGGCA 
GATCCGTACT CCACCCTCAG TCGAGTGGGA AGTGTAAACT CCACCCTGAA ACGCTCAGAC 
ACCCGTGATT CCTGTTGTCA AACAGAAGAG GTGAAGATTG TTCCTCCCTC AGTACGCAGA 
ATCAGAGCAC AAAGAGGCCA TGGAATAGCT GCCCAAATGG CCAGTGTCTC CTCCTCAAGC 
AGCATTTCCA CCATAAGTGA CTGTAATGGC ATTAGCTACA CACAGCTTAA TGACAGTGAT 
CAGAGCTTTC AAAGTTTACC AAGACAAGGT TCTCGCATCC CGCAACAACA CAGTGAAACA 
AAGTACAATA GCTCCCCCTA CAGGGCGAAT TCTGGTTCTA CAAGCCCCTT GTCCTACCAT 
TTTAACATGC AGCATGACTC TTCATCATCG CTGCATATGC TGTCTGACTC GAGAAGCAAG 
CTTACTGAAA ATCTAAATTA CGAAGGCTCT GATAAAAATA ATACCTCAAG CTTGTTTAGT 
CTCAGTGGTG CTCTACAGAA CTCCATTCCT TATGGATACA AAAGGACAGA GGACAACCAA 
ATGCAATCAC CTCATTCGTC AGTCCAGTTG CTCAATTCTG AGGATGCCTC ATTGTCGGGT 
GTCTCCTCGT GTTATGAATC CACAGCGTCC CTCTGTACAG GTATTCATAC AGAGACTGAG 
TCCCAGTGCA GCACACTTGA TGGCAGAAAC TGCTGCAGTC CTAACTGTGT CCGCAGGGAT 
TCCAACTTCT CAGAGAGCAG CAACCAAAGC TGCAGCACAC TAACCACGGA CCAATGGACG 
TATGAAGTTT CTCCAAAAGA CAATGTTACT TCAAGTTGCT CCTCACCTGT CAGCCATATA 
TACCACGCAG AGGAACATTC TCCCAGCAAG ACAGATACAA GCTCATTGTT CTCTGTTGAC 
AATGAAGGTT ACTACACCTC CATGCGCCTA GACTCTGGTT TGAAGTCACA CAGTCACGGC 
TGCATCAATA AAGCAGGAGA ACAAAAGCAC AGTTTGTATG AGTGCAAAGA CCACCACAGT 
CAAAGTGATA CTGCAAGTCT GCATAGCAAC CGCTCCCTGA CTCGTAGCAT CTCTCTGCGA 
AAAGCCAGGA AGCCCCCACT CCCTCCAGAA AGAACCGACT CTTTAAGGCG TAAACCCCAG 
AGGAAGAGTC GCCACAGTGG TTCAGTCCTT AATGAGCAGT TAATCTCTAG TCTCCAGCAG 
TCCTTACAAC TTAACCTAAA TACTCAAACT ACATCTTGCT CTGAACAGTC ACCCTGTGGT 
AGGTTTGAAG ACCCTTGGGT CCTCCGACCG AGAAGTCAGA GTATCATAAG TGCAGCTAGC 
AGTGGGGTAT CTGCACCAGC TGCTGTATGT CCGGTCACAC CAACACACAG TGACAGCAGC 
AGTCAGCGCT CAGACTATGC AGAGTCATGG GACTTCTACA TGGATTGCCC ATGTCCACGG 
TCTGACCAGA GTCTTTCCTC ACAAATCACA AGGTCTGCAA GTGCTGAGGA GAATCATGGA 
GTCATAGGTA ATGCATCCTA CAACAGTAAA TTCCCATCAT CCCCCTTCAG CAATAGTCAG 
TCAAAATTGA CCTCATCTCC TGACAAAGTC CATCGACTCA CGTCACCATC AAGTGGCTAC 
TCAAGTCAGT CTAACACTCC AACTGCAGGC ACTCCAGTTA CGTCTCTAAT AAGAGCAAAG 
TCTCCTGCGG GGAGACCGAA ACCAAAAGTG CCAGAAAGAA AGTCCTCTCT ACGATCTTCT 
ATATCATCCT CTTCTACATC CCTGTCCTCC AACACCTCAG ATTCCATCAG GAACATTCCT 
CCACCTCCAC CTTTACCTGA CATGACAACT GCATTATGTG GGTCGCCCAG TTCTGCTTCT 
TTCTCTCCAA CAGCGGCTAA AATAGACCCA ATACCCTCTT TTACATCTCT ACAGGATGTA 
TCATCTGCCT TGGAAGATGG GCAAAATACA CTTTCAGATA CATCTGCAAC ACCTGAGGAA 
TGTGATAGTC CTGATTTTCC CCCTCCCCAT TCAAATGTTT TTGAATCTCT GTCAGGCAAT 
GTAGATACCA ACAGCCTCCC ACCTCCTCCA CTTCTGCTTT GCAAACCTTC ACTGTGCATT 
TCATCTCTTC TTCCCCCTCC TCCTCCACCT CCTCCACTAT TACTTGAGAC CAGTCTTCAA 
CCAGCCACCA TTTTGAAGAC AGAGAGCAGT CTTACACCTA CAACAGCTGT AAAATGTGAA 
AAAGAAGTAA ACATCAGTCA CACTGGAGAG CAAGAAAAGA CTCAGAGACC AATAATCACT 
GCCCAGGCCC TTCAGATGGT GCAGTTGCGA CCTGTTAAGC TCAAGCAAAT AGAAACTATT 
TTTACATCTG TCAGTTTTGA TCATTCTGAT CACCAAGCAC AAAACCATTC TGATACAAAC 
TCAGGAAAGT CCCTGGAAAT TGATAATACA GGGCAAACAG TGACCGTCAA TTGTGTTACT 
CAGTCAGAAA ATAGTATGGA AACATCTGAA TGTGAATATT CGGTGAACAG ATTCATAGAC 
GATCTCCCTG AGGTATCAAC TAGCACTCTA CCCCCTGACC TAGATGAACA ATTAAAAAAA 
ACTGAGCAAA ATAATCCAGT GCACCTCTCT TCCAAGTCCA CAAATTCCTT AGCTTCTCCT 
CTGAGTTCTC CAATGAAACA GAAGCCTCCT ATATCACCCA AAAAACCCAA TCTTTCCCTT 
ATTATACCAC CCATAATGCA CTTGCCATTT TCCAACGGAC TCAAGCTTCA GCAGGTTCAT 
GAAGCACAAG AGACACTAGA GTCAGAGCAA GTGAATGGTG CATCCTCGAC AAACACAGTT 
AATTTGCAAG CAATTCCTTT AGACCTTGAG GTAGAGGAAG AGAGTGACTC AGACTTATCA 
ACCCCTGTGG TCAAGAGCAG ACTATCTTTC TGCAGCGAGT TATCATCAAG CAGTTTCCAA 
GACCTGCAAC TTTCTGATTA CATTCTTAAT GAGCATGACC TCGGCCTGAG CGATAAAGAC 
TTTGGGCTCT GTGATGATAA AAGCACATCA GATGATGGTT CAAGCAGCAG CTCAGGATCC 
ATCAGCTTTA AAGAGGATGA ACATGAGGAA AACGGTGCTG TGTTTGACTC AATCACTGAA 
AGCACATCTG ACATTAACGC TAGCGGAGAA GCTGTGGAGG GGATGGTGAC GCCTGTTCGC 
CCTCGTACTA CAGAGGACCT CTTTGCTGCA ATTCATAGGT CAAAAAGGAA GGTCCTGGGC 
CGTGGCGATT CTGAAGATGA ACGCTGTCGT AACCTCCTCC CTTCCCCACC TGTCACTCCA 
ACTGGCTCCT GCCCCAGCTT GCCACGTCAG ACCAGCTCCA TCCAGCGGAA CCTACGTCGC 
TCCACCACAA GCAATGACAG TTTTAAAGCT TTGCTACTCA AGAAGGGCAG CCGCTCAGAG 
TCCAGCTTTC GCATGTCAGC CGCTGAGATT CTCAAATGCA CAGACCCCCG TTTCCAAAGG 
GCTAATTCAG AGAATGCACT AGCTGACGGA CTGTCCTCTC CGACAAGCAA CAGGAGAGCA 
CATGAAGAGT GGGCACGGAT GGAGGGAGCT TTGCCTCGCT TTACCACTGG ACAAACTATC 
CCAAAATATG GGCGCTCGCG CACACCACCT TCCGCAGCCA GCAGCCGGTA TAACAGCCGC 
AGCCGGATTA CCAGTAGGCC AATGACTGCC ATCTGTGAAC GAGATGGAGA GGTGGCTGAA 
TCTACAGACT GCTACATAAG TAGCGAGAAT CATTTTGTCT TGGCTTTATC CTCCAGCAGC 
ATGGTCTGCG CTCAAGGCAG CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_689979.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_695071.7 Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa145950
Clone ID Related Accession (Same CDS sequence)	XM_689979.9
Accession Version	XM_689979.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394996.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689979.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGCGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AGAAAAGAAA AAGAAGGCTT CCGATTCACC TAATGAAGAG 
GATAAACTTG TGTACTCAAT GCCGCCACAA ACCCCTCTGC TGGAGCATGG CTCTGACATG 
GACATCCAGA GAAGCTGGAC CAGCTGTCTA CCTTTGCCCA CTCCCGAGGA GAAGATGAGA 
CTGCAGGCCC AATCGGTGGC TACTGATATA ATTCCTATCA ACATTACAGG TGAGAACTTT 
GACCGCCAGG CCAGCTTTCG CCGCTCCATG GCAAACACTG ATACTATTAT ACGAAGACCT 
AAGAGAGTTC AAAGAAGAAA GACAATAACA GGAGTGCCTG ACAACATCCA GACTGAACTA 
GCTAATAAAG AACAAGCTGA CTCCAGAATA CATTCGAGTA TGGCAGATCC GTACTCCACC 
CTCAGTCGAG TGGGAAGTGT AAACTCCACC CTGAAACGCT CAGACACCCG TGATTCCTGT 
TGTCAAACAG AAGAGGTGAA GATTGTTCCT CCCTCAGTAC GCAGAATCAG AGCACAAAGA 
GGCCATGGAA TAGCTGCCCA AATGGCCAGT GTCTCCTCCT CAAGCAGCAT TTCCACCATA 
AGTGACTGTA ATGGCATTAG CTACACACAG CTTAATGACA GTGATCAGAG CTTTCAAAGT 
TTACCAAGAC AAGGTTCTCG CATCCCGCAA CAACACAGTG AAACAAAGTA CAATAGCTCC 
CCCTACAGGG CGAATTCTGG TTCTACAAGC CCCTTGTCCT ACCATTTTAA CATGCAGCAT 
GACTCTTCAT CATCGCTGCA TATGCTGTCT GACTCGAGAA GCAAGCTTAC TGAAAATCTA 
AATTACGAAG GCTCTGATAA AAATAATACC TCAAGCTTGT TTAGTCTCAG TGGTGCTCTA 
CAGAACTCCA TTCCTTATGG ATACAAAAGG ACAGAGGACA ACCAAATGCA ATCACCTCAT 
TCGTCAGTCC AGTTGCTCAA TTCTGAGGAT GCCTCATTGT CGGGTGTCTC CTCGTGTTAT 
GAATCCACAG CGTCCCTCTG TACAGGTATT CATACAGAGA CTGAGTCCCA GTGCAGCACA 
CTTGATGGCA GAAACTGCTG CAGTCCTAAC TGTGTCCGCA GGGATTCCAA CTTCTCAGAG 
AGCAGCAACC AAAGCTGCAG CACACTAACC ACGGACCAAT GGACGTATGA AGTTTCTCCA 
AAAGACAATG TTACTTCAAG TTGCTCCTCA CCTGTCAGCC ATATATACCA CGCAGAGGAA 
CATTCTCCCA GCAAGACAGA TACAAGCTCA TTGTTCTCTG TTGACAATGA AGGTTACTAC 
ACCTCCATGC GCCTAGACTC TGGTTTGAAG TCACACAGTC ACGGCTGCAT CAATAAAGCA 
GGAGAACAAA AGCACAGTTT GTATGAGTGC AAAGACCACC ACAGTCAAAG TGATACTGCA 
AGTCTGCATA GCAACCGCTC CCTGACTCGT AGCATCTCTC TGCGAAAAGC CAGGAAGCCC 
CCACTCCCTC CAGAAAGAAC CGACTCTTTA AGGCGTAAAC CCCAGAGGAA GAGTCGCCAC 
AGTGGTTCAG TCCTTAATGA GCAGTTAATC TCTAGTCTCC AGCAGTCCTT ACAACTTAAC 
CTAAATACTC AAACTACATC TTGCTCTGAA CAGTCACCCT GTGGTAGGTT TGAAGACCCT 
TGGGTCCTCC GACCGAGAAG TCAGAGTATC ATAAGTGCAG CTAGCAGTGG GGTATCTGCA 
CCAGCTGCTG TATGTCCGGT CACACCAACA CACAGTGACA GCAGCAGTCA GCGCTCAGAC 
TATGCAGAGT CATGGGACTT CTACATGGAT TGCCCATGTC CACGGTCTGA CCAGAGTCTT 
TCCTCACAAA TCACAAGGTC TGCAAGTGCT GAGGAGAATC ATGGAGTCAT AGGTAATGCA 
TCCTACAACA GTAAATTCCC ATCATCCCCC TTCAGCAATA GTCAGTCAAA ATTGACCTCA 
TCTCCTGACA AAGTCCATCG ACTCACGTCA CCATCAAGTG GCTACTCAAG TCAGTCTAAC 
ACTCCAACTG CAGGCACTCC AGTTACGTCT CTAATAAGAG CAAAGTCTCC TGCGGGGAGA 
CCGAAACCAA AAGTGCCAGA AAGAAAGTCC TCTCTACGAT CTTCTATATC ATCCTCTTCT 
ACATCCCTGT CCTCCAACAC CTCAGATTCC ATCAGGAACA TTCCTCCACC TCCACCTTTA 
CCTGACATGA CAACTGCATT ATGTGGGTCG CCCAGTTCTG CTTCTTTCTC TCCAACAGCG 
GCTAAAATAG ACCCAATACC CTCTTTTACA TCTCTACAGG ATGTATCATC TGCCTTGGAA 
GATGGGCAAA ATACACTTTC AGATACATCT GCAACACCTG AGGAATGTGA TAGTCCTGAT 
TTTCCCCCTC CCCATTCAAA TGTTTTTGAA TCTCTGTCAG GCAATGTAGA TACCAACAGC 
CTCCCACCTC CTCCACTTCT GCTTTGCAAA CCTTCACTGT GCATTTCATC TCTTCTTCCC 
CCTCCTCCTC CACCTCCTCC ACTATTACTT GAGACCAGTC TTCAACCAGC CACCATTTTG 
AAGACAGAGA GCAGTCTTAC ACCTACAACA GCTGTAAAAT GTGAAAAAGA AGTAAACATC 
AGTCACACTG GAGAGCAAGA AAAGACTCAG AGACCAATAA TCACTGCCCA GGCCCTTCAG 
ATGGTGCAGT TGCGACCTGT TAAGCTCAAG CAAATAGAAA CTATTTTTAC ATCTGTCAGT 
TTTGATCATT CTGATCACCA AGCACAAAAC CATTCTGATA CAAACTCAGG AAAGTCCCTG 
GAAATTGATA ATACAGGGCA AACAGTGACC GTCAATTGTG TTACTCAGTC AGAAAATAGT 
ATGGAAACAT CTGAATGTGA ATATTCGGTG AACAGATTCA TAGACGATCT CCCTGAGGTA 
TCAACTAGCA CTCTACCCCC TGACCTAGAT GAACAATTAA AAAAAACTGA GCAAAATAAT 
CCAGTGCACC TCTCTTCCAA GTCCACAAAT TCCTTAGCTT CTCCTCTGAG TTCTCCAATG 
AAACAGAAGC CTCCTATATC ACCCAAAAAA CCCAATCTTT CCCTTATTAT ACCACCCATA 
ATGCACTTGC CATTTTCCAA CGGACTCAAG CTTCAGCAGG TTCATGAAGC ACAAGAGACA 
CTAGAGTCAG AGCAAGTGAA TGGTGCATCC TCGACAAACA CAGTTAATTT GCAAGCAATT 
CCTTTAGACC TTGAGGTAGA GGAAGAGAGT GACTCAGACT TATCAACCCC TGTGGTCAAG 
AGCAGACTAT CTTTCTGCAG CGAGTTATCA TCAAGCAGTT TCCAAGACCT GCAACTTTCT 
GATTACATTC TTAATGAGCA TGACCTCGGC CTGAGCGATA AAGACTTTGG GCTCTGTGAT 
GATAAAAGCA CATCAGATGA TGGTTCAAGC AGCAGCTCAG GATCCATCAG CTTTAAAGAG 
GATGAACATG AGGAAAACGG TGCTGTGTTT GACTCAATCA CTGAAAGCAC ATCTGACATT 
AACGCTAGCG GAGAAGCTGT GGAGGGGATG GTGACGCCTG TTCGCCCTCG TACTACAGAG 
GACCTCTTTG CTGCAATTCA TAGGTCAAAA AGGAAGGTCC TGGGCCGTGG CGATTCTGAA 
GATGAACGCT GTCGTAACCT CCTCCCTTCC CCACCTGTCA CTCCAACTGG CTCCTGCCCC 
AGCTTGCCAC GTCAGACCAG CTCCATCCAG CGGAACCTAC GTCGCTCCAC CACAAGCAAT 
GACAGTTTTA AAGCTTTGCT ACTCAAGAAG GGCAGCCGCT CAGAGTCCAG CTTTCGCATG 
TCAGCCGCTG AGATTCTCAA ATGCACAGAC CCCCGTTTCC AAAGGGCTAA TTCAGAGAAT 
GCACTAGCTG ACGGACTGTC CTCTCCGACA AGCAACAGGA GAGCACATGA AGAGTGGGCA 
CGGATGGAGG GAGCTTTGCC TCGCTTTACC ACTGGACAAA CTATCCCAAA ATATGGGCGC 
TCGCGCACAC CACCTTCCGC AGCCAGCAGC CGGTATAACA GCCGCAGCCG GATTACCAGT 
AGGCCAATGA CTGCCATCTG TGAACGAGAT GGAGAGGTGG CTGAATCTAC AGACTGCTAC 
ATAAGTAGCG AGAATCATTT TGTCTTGGCT TTATCCTCCA GCAGCATGGT CTGCGCTCAA 
GGCAGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695071.7
CDS	180..4691
Translation	MVFIGTTLKSVIKYFKKKAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTEKKKKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCPRSDQSLSSQITRSASAEENHGVIGNASYNSKFPSSPFSNSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVHEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPVRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSNRRAHEEWARMEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYISSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_689979.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689979.9

ORF Insert Sequence:

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGCGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AGAAAAGAAA AAGAAGGCTT CCGATTCACC TAATGAAGAG 
GATAAACTTG TGTACTCAAT GCCGCCACAA ACCCCTCTGC TGGAGCATGG CTCTGACATG 
GACATCCAGA GAAGCTGGAC CAGCTGTCTA CCTTTGCCCA CTCCCGAGGA GAAGATGAGA 
CTGCAGGCCC AATCGGTGGC TACTGATATA ATTCCTATCA ACATTACAGG TGAGAACTTT 
GACCGCCAGG CCAGCTTTCG CCGCTCCATG GCAAACACTG ATACTATTAT ACGAAGACCT 
AAGAGAGTTC AAAGAAGAAA GACAATAACA GGAGTGCCTG ACAACATCCA GACTGAACTA 
GCTAATAAAG AACAAGCTGA CTCCAGAATA CATTCGAGTA TGGCAGATCC GTACTCCACC 
CTCAGTCGAG TGGGAAGTGT AAACTCCACC CTGAAACGCT CAGACACCCG TGATTCCTGT 
TGTCAAACAG AAGAGGTGAA GATTGTTCCT CCCTCAGTAC GCAGAATCAG AGCACAAAGA 
GGCCATGGAA TAGCTGCCCA AATGGCCAGT GTCTCCTCCT CAAGCAGCAT TTCCACCATA 
AGTGACTGTA ATGGCATTAG CTACACACAG CTTAATGACA GTGATCAGAG CTTTCAAAGT 
TTACCAAGAC AAGGTTCTCG CATCCCGCAA CAACACAGTG AAACAAAGTA CAATAGCTCC 
CCCTACAGGG CGAATTCTGG TTCTACAAGC CCCTTGTCCT ACCATTTTAA CATGCAGCAT 
GACTCTTCAT CATCGCTGCA TATGCTGTCT GACTCGAGAA GCAAGCTTAC TGAAAATCTA 
AATTACGAAG GCTCTGATAA AAATAATACC TCAAGCTTGT TTAGTCTCAG TGGTGCTCTA 
CAGAACTCCA TTCCTTATGG ATACAAAAGG ACAGAGGACA ACCAAATGCA ATCACCTCAT 
TCGTCAGTCC AGTTGCTCAA TTCTGAGGAT GCCTCATTGT CGGGTGTCTC CTCGTGTTAT 
GAATCCACAG CGTCCCTCTG TACAGGTATT CATACAGAGA CTGAGTCCCA GTGCAGCACA 
CTTGATGGCA GAAACTGCTG CAGTCCTAAC TGTGTCCGCA GGGATTCCAA CTTCTCAGAG 
AGCAGCAACC AAAGCTGCAG CACACTAACC ACGGACCAAT GGACGTATGA AGTTTCTCCA 
AAAGACAATG TTACTTCAAG TTGCTCCTCA CCTGTCAGCC ATATATACCA CGCAGAGGAA 
CATTCTCCCA GCAAGACAGA TACAAGCTCA TTGTTCTCTG TTGACAATGA AGGTTACTAC 
ACCTCCATGC GCCTAGACTC TGGTTTGAAG TCACACAGTC ACGGCTGCAT CAATAAAGCA 
GGAGAACAAA AGCACAGTTT GTATGAGTGC AAAGACCACC ACAGTCAAAG TGATACTGCA 
AGTCTGCATA GCAACCGCTC CCTGACTCGT AGCATCTCTC TGCGAAAAGC CAGGAAGCCC 
CCACTCCCTC CAGAAAGAAC CGACTCTTTA AGGCGTAAAC CCCAGAGGAA GAGTCGCCAC 
AGTGGTTCAG TCCTTAATGA GCAGTTAATC TCTAGTCTCC AGCAGTCCTT ACAACTTAAC 
CTAAATACTC AAACTACATC TTGCTCTGAA CAGTCACCCT GTGGTAGGTT TGAAGACCCT 
TGGGTCCTCC GACCGAGAAG TCAGAGTATC ATAAGTGCAG CTAGCAGTGG GGTATCTGCA 
CCAGCTGCTG TATGTCCGGT CACACCAACA CACAGTGACA GCAGCAGTCA GCGCTCAGAC 
TATGCAGAGT CATGGGACTT CTACATGGAT TGCCCATGTC CACGGTCTGA CCAGAGTCTT 
TCCTCACAAA TCACAAGGTC TGCAAGTGCT GAGGAGAATC ATGGAGTCAT AGGTAATGCA 
TCCTACAACA GTAAATTCCC ATCATCCCCC TTCAGCAATA GTCAGTCAAA ATTGACCTCA 
TCTCCTGACA AAGTCCATCG ACTCACGTCA CCATCAAGTG GCTACTCAAG TCAGTCTAAC 
ACTCCAACTG CAGGCACTCC AGTTACGTCT CTAATAAGAG CAAAGTCTCC TGCGGGGAGA 
CCGAAACCAA AAGTGCCAGA AAGAAAGTCC TCTCTACGAT CTTCTATATC ATCCTCTTCT 
ACATCCCTGT CCTCCAACAC CTCAGATTCC ATCAGGAACA TTCCTCCACC TCCACCTTTA 
CCTGACATGA CAACTGCATT ATGTGGGTCG CCCAGTTCTG CTTCTTTCTC TCCAACAGCG 
GCTAAAATAG ACCCAATACC CTCTTTTACA TCTCTACAGG ATGTATCATC TGCCTTGGAA 
GATGGGCAAA ATACACTTTC AGATACATCT GCAACACCTG AGGAATGTGA TAGTCCTGAT 
TTTCCCCCTC CCCATTCAAA TGTTTTTGAA TCTCTGTCAG GCAATGTAGA TACCAACAGC 
CTCCCACCTC CTCCACTTCT GCTTTGCAAA CCTTCACTGT GCATTTCATC TCTTCTTCCC 
CCTCCTCCTC CACCTCCTCC ACTATTACTT GAGACCAGTC TTCAACCAGC CACCATTTTG 
AAGACAGAGA GCAGTCTTAC ACCTACAACA GCTGTAAAAT GTGAAAAAGA AGTAAACATC 
AGTCACACTG GAGAGCAAGA AAAGACTCAG AGACCAATAA TCACTGCCCA GGCCCTTCAG 
ATGGTGCAGT TGCGACCTGT TAAGCTCAAG CAAATAGAAA CTATTTTTAC ATCTGTCAGT 
TTTGATCATT CTGATCACCA AGCACAAAAC CATTCTGATA CAAACTCAGG AAAGTCCCTG 
GAAATTGATA ATACAGGGCA AACAGTGACC GTCAATTGTG TTACTCAGTC AGAAAATAGT 
ATGGAAACAT CTGAATGTGA ATATTCGGTG AACAGATTCA TAGACGATCT CCCTGAGGTA 
TCAACTAGCA CTCTACCCCC TGACCTAGAT GAACAATTAA AAAAAACTGA GCAAAATAAT 
CCAGTGCACC TCTCTTCCAA GTCCACAAAT TCCTTAGCTT CTCCTCTGAG TTCTCCAATG 
AAACAGAAGC CTCCTATATC ACCCAAAAAA CCCAATCTTT CCCTTATTAT ACCACCCATA 
ATGCACTTGC CATTTTCCAA CGGACTCAAG CTTCAGCAGG TTCATGAAGC ACAAGAGACA 
CTAGAGTCAG AGCAAGTGAA TGGTGCATCC TCGACAAACA CAGTTAATTT GCAAGCAATT 
CCTTTAGACC TTGAGGTAGA GGAAGAGAGT GACTCAGACT TATCAACCCC TGTGGTCAAG 
AGCAGACTAT CTTTCTGCAG CGAGTTATCA TCAAGCAGTT TCCAAGACCT GCAACTTTCT 
GATTACATTC TTAATGAGCA TGACCTCGGC CTGAGCGATA AAGACTTTGG GCTCTGTGAT 
GATAAAAGCA CATCAGATGA TGGTTCAAGC AGCAGCTCAG GATCCATCAG CTTTAAAGAG 
GATGAACATG AGGAAAACGG TGCTGTGTTT GACTCAATCA CTGAAAGCAC ATCTGACATT 
AACGCTAGCG GAGAAGCTGT GGAGGGGATG GTGACGCCTG TTCGCCCTCG TACTACAGAG 
GACCTCTTTG CTGCAATTCA TAGGTCAAAA AGGAAGGTCC TGGGCCGTGG CGATTCTGAA 
GATGAACGCT GTCGTAACCT CCTCCCTTCC CCACCTGTCA CTCCAACTGG CTCCTGCCCC 
AGCTTGCCAC GTCAGACCAG CTCCATCCAG CGGAACCTAC GTCGCTCCAC CACAAGCAAT 
GACAGTTTTA AAGCTTTGCT ACTCAAGAAG GGCAGCCGCT CAGAGTCCAG CTTTCGCATG 
TCAGCCGCTG AGATTCTCAA ATGCACAGAC CCCCGTTTCC AAAGGGCTAA TTCAGAGAAT 
GCACTAGCTG ACGGACTGTC CTCTCCGACA AGCAACAGGA GAGCACATGA AGAGTGGGCA 
CGGATGGAGG GAGCTTTGCC TCGCTTTACC ACTGGACAAA CTATCCCAAA ATATGGGCGC 
TCGCGCACAC CACCTTCCGC AGCCAGCAGC CGGTATAACA GCCGCAGCCG GATTACCAGT 
AGGCCAATGA CTGCCATCTG TGAACGAGAT GGAGAGGTGG CTGAATCTAC AGACTGCTAC 
ATAAGTAGCG AGAATCATTT TGTCTTGGCT TTATCCTCCA GCAGCATGGT CTGCGCTCAA 
GGCAGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158819.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158876.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.

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CloneID	ODa22649
Clone ID Related Accession (Same CDS sequence)	XM_005158819.3 , XM_021467435.1
Accession Version	XM_005158819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158876.1
CDS	265..5079
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158819.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158819.3

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467435.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323110.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.

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CloneID	ODa22649
Clone ID Related Accession (Same CDS sequence)	XM_005158819.3 , XM_021467435.1
Accession Version	XM_021467435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323110.1
CDS	286..5100
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467435.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467435.1

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158820.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158877.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22650
Clone ID Related Accession (Same CDS sequence)	XM_005158820.3 , XM_021467436.1
Accession Version	XM_005158820.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC ATAAGAGAAC AGTGGAGCCC CGGAGCCTGG GCAGAATAAA CAGGGATCGA 
CTGGACGTTC AGGAGGATGT GTTTTCTTCT CTGGATGCTG TGAACTGTCA GGCTTTGATG 
AACGTCCTCC GTCAGCTGTG CGATGTGTCC CGGCACGCGG GCTGCATGTT TGTGGAGCTG 
CAGACTGAAG CAGCGCTGGT CATCAATAAA GCCTCAGCGC TGCAGCACAG ACTGGACACA 
CTGCAGGACA CTGTGCGACA CCTCAACCAC AGGAGGACCA CGACCCCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158877.1
CDS	195..5009
Translation	MPFHKRTVEPRSLGRINRDRLDVQEDVFSSLDAVNCQALMNVLRQLCDVSRHAGCMFVELQTEAALVINKASALQHRLDTLQDTVRHLNHRRTTTPVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158820.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158820.3

ORF Insert Sequence:

ATGCCTTTTC ATAAGAGAAC AGTGGAGCCC CGGAGCCTGG GCAGAATAAA CAGGGATCGA 
CTGGACGTTC AGGAGGATGT GTTTTCTTCT CTGGATGCTG TGAACTGTCA GGCTTTGATG 
AACGTCCTCC GTCAGCTGTG CGATGTGTCC CGGCACGCGG GCTGCATGTT TGTGGAGCTG 
CAGACTGAAG CAGCGCTGGT CATCAATAAA GCCTCAGCGC TGCAGCACAG ACTGGACACA 
CTGCAGGACA CTGTGCGACA CCTCAACCAC AGGAGGACCA CGACCCCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467436.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323111.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22650
Clone ID Related Accession (Same CDS sequence)	XM_005158820.3 , XM_021467436.1
Accession Version	XM_021467436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTC ATAAGAGAAC AGTGGAGCCC CGGAGCCTGG GCAGAATAAA CAGGGATCGA 
CTGGACGTTC AGGAGGATGT GTTTTCTTCT CTGGATGCTG TGAACTGTCA GGCTTTGATG 
AACGTCCTCC GTCAGCTGTG CGATGTGTCC CGGCACGCGG GCTGCATGTT TGTGGAGCTG 
CAGACTGAAG CAGCGCTGGT CATCAATAAA GCCTCAGCGC TGCAGCACAG ACTGGACACA 
CTGCAGGACA CTGTGCGACA CCTCAACCAC AGGAGGACCA CGACCCCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323111.1
CDS	182..4996
Translation	MPFHKRTVEPRSLGRINRDRLDVQEDVFSSLDAVNCQALMNVLRQLCDVSRHAGCMFVELQTEAALVINKASALQHRLDTLQDTVRHLNHRRTTTPVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467436.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467436.1

ORF Insert Sequence:

ATGCCTTTTC ATAAGAGAAC AGTGGAGCCC CGGAGCCTGG GCAGAATAAA CAGGGATCGA 
CTGGACGTTC AGGAGGATGT GTTTTCTTCT CTGGATGCTG TGAACTGTCA GGCTTTGATG 
AACGTCCTCC GTCAGCTGTG CGATGTGTCC CGGCACGCGG GCTGCATGTT TGTGGAGCTG 
CAGACTGAAG CAGCGCTGGT CATCAATAAA GCCTCAGCGC TGCAGCACAG ACTGGACACA 
CTGCAGGACA CTGTGCGACA CCTCAACCAC AGGAGGACCA CGACCCCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGGTCA AAAAGGAAGG TCCTGGGCCG TGGCGATTCT 
GAAGATGAAC GCTGCCGTAA CCTCCTTCCT TCCCCACCTG TCACTCCAAC TGGCTCCTGC 
CCCAGCTTGC CACGTCAGAC CAGCTCCATC CAGCGGAACC TACGTCGCTC CACCACAAGC 
AATGACAGTT TTAAAGCTTT GCTACTCAAG AAGGGCAGCC GCTCAGAGTC CAGCTTTCGC 
ATGTCAGCCG CTGAGATTCT CAAATGCACA GACCCCCGTT TCCAAAGGGC TAATTCAGAA 
AATGCACTAG CTGACGGACT GTCCTCTCCG ACAAGCAGCA GGAGAGCACA TGAAGAGTGG 
GCACGGACGG AGGGAGCTTT GCCTCGCTTT ACCACTGGAC AAACTATCCC AAAATATGGG 
CGCTCGCGCA CACCACCTTC CGCAGCCAGC AGCCGGTATA ACAGCCGCAG CCGGATTACC 
AGTAGGCCAA TGACTGCCAT CTGTGAACGA GATGGAGAGG TGGCTGAATC TACAGACTGC 
TACTTAAGTA GCGAGAATCA TTTTGTCTTG GCTTTATCCT CCAGCAGCAT GGTCTGCGCT 
CAAGGCAGCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158821.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158878.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.

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CloneID	ODa22651
Clone ID Related Accession (Same CDS sequence)	XM_005158821.3 , XM_021467437.1
Accession Version	XM_005158821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158821.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGGCTTCC GATTCACCTA ATGAAGAGGA TAAACTTGTG 
TACTCAATGC CGCCACAAAC CCCTCTGCTG GAGCATGGCT CTGACATGGA CATCCAGAGA 
AGCTGGACCA GCTGTCTACC TTTGCCCACT CCCGAGGAGA AGATGAGACT GCAGGCCCAA 
TCGGTGGCTA CTGATATAAT TCCTATCAAC ATTACAGGTG AGAACTTTGA CCGCCAGGCC 
AGCTTTCGCC GCTCCATGGC AAACACTGAT ACTATTATAC GAAGACCTAA GAGAGTTCAA 
AGAAGAAAGA CAATAACAGG AGTGCCTGAC AACATCCAGA CTGAACTAGC AAATAAAGAA 
CAAGCTGACT CCAGAATACA TTCGAGTATG GCAGATCCAT ACTCCACCCT CAGTCGAGTG 
GGAAGTGTAA ACTCCACCCT GAAACGCTCA GACACCCGTG ATTCCTGTTG TCAAACAGAA 
GAGGTGAAGA TTGTTCCTCC CTCAGTACGC AGAATCAGAG CACAAAGAGG CCATGGAATA 
GCTGCCCAAA TGGCCAGTGT CTCCTCCTCA AGCAGCATTT CCACCATAAG TGACTGTAAT 
GGCATTAGCT ACACACAGCT TAATGACAGT GATCAGAGCT TTCAAAGTTT ACCAAGACAA 
GGTTCTCGCA TCCCGCAACA ACACAGTGAA ACAAAGTACA ATAGCTCCCC CTACAGGGCG 
AATTCTGGTT CTACAAGCCC CTTGTCCTAC CATTTTAACA TGCAGCATGA CTCTTCATCA 
TCGCTGCATA TGCTGTCTGA CTCGAGAAGC AAGCTTACTG AAAATCTAAA TTACGAAGGC 
TCTGATAAAA ATAATACCTC AAGCTTGTTT AGTCTCAGTG GTGCTCTACA GAACTCCATT 
CCTTATGGAT ACAAAAGGAC AGAGGACAAC CAAATGCAAT CACCTCATTC GTCAGTCCAG 
TTGCTCAATT CTGAGGATGC CTCATTGTCG GGTGTCTCCT CGTGTTATGA ATCCACAGCG 
TCCCTCTGTA CAGGTATTCA TACAGAGACT GAGTCCCAGT GCAGCACACT TGATGGCAGA 
AACTGCTGCA GTCCTAACTG TGTCCGCAGG GATTCCAACT TCTCAGAGAG CAGCAACCAA 
AGCTGCAGCA CACTAACCAC GGACCAATGG ACGTATGAAG TTTCTCCAAA AGACAATGTT 
ACTTCAAGTT GCTCCTCACC TGTCAGCCAT ATATACCACG CAGAGGAACA TTCTCCCAGC 
AAGACAGATA CAAGCTCATT GTTCTCTGTT GACAATGAAG GTTACTACAC CTCCATGCGC 
CTAGACTCTG GTTTGAAGTC ACACAGTCAC GGCTGCATCA ATAAAGCAGG AGAACAAAAG 
CACAGTTTGT ATGAGTGCAA AGACCACCAC AGTCAAAGTG ATACTGCAAG TCTGCATAGC 
AACCGCTCCC TGACTCGTAG CATCTCTCTG CGAAAAGCCA GGAAGCCCCC ACTCCCTCCA 
GAAAGAACCG ACTCTTTAAG GCGTAAACCC CAGAGGAAGA GTCGCCACAG TGGTTCAGTC 
CTTAATGAGC AGTTAATCTC TAGTCTCCAG CAGTCCTTAC AACTTAACCT AAATACTCAA 
ACTACATCTT GCTCTGAACA GTCACCCTGT GGTAGGTTTG AAGACCCTTG GGTCCTCCGA 
CCGAGAAGTC AGAGTATCAT AAGTGCAGCT AGCAGTGGGG TATCTGCACC AGCTGCTGTA 
TGTCCGGTCA CACCAACACA CAGTGACAGC AGCAGTCAGC GCTCAGACTA TGCAGAGTCA 
TGGGACTTCT ACATGGATTG CCCATGTACA CGGTCTGACC AGAGTCTTTC CTCACAAATC 
ACAAGGTCTG CAAGTGCTGA GGAGAATCAT GGAGTCATAG GTAATGCATC CTACAACAGT 
GAATTCCCAT CATCCCCCTT CAGCAGTAGT CAGTCAAAAT TGACCTCATC TCCTGACAAA 
GTCCATCGAC TCACGTCACC ATCAAGTGGC TACTCAAGTC AGTCTAACAC TCCAACTGCA 
GGCACTCCAG TTACGTCTCT AATAAGAGCA AAGTCTCCTG CGGGGAGACC GAAACCAAAA 
GTGCCAGAAA GAAAGTCCTC TCTACGATCT TCTATATCAT CCTCTTCTAC ATCCCTGTCC 
TCCAACACCT CAGATTCCAT CAGGAACATT CCTCCACCTC CACCTTTACC TGACATGACA 
ACTGCATTAT GTGGGTCGCC CAGTTCTGCT TCTTTCTCTC CAACAGCGGC TAAAATAGAC 
CCAATACCCT CTTTTACATC TCTACAGGAT GTATCATCTG CCTTGGAAGA TGGGCAAAAT 
ACACTTTCAG ATACATCTGC AACACCTGAG GAATGTGATA GTCCTGATTT TCCCCCTCCC 
CATTCAAATG TTTTTGAATC TCTGTCAGGC AATGTAGATA CCAACAGCCT CCCACCTCCT 
CCACTTCTGC TTTGCAAACC TTCACTGTGC ATTTCATCTC TTCTTCCCCC TCCTCCTCCA 
CCTCCTCCAC TATTACTTGA GACCAGTCTT CAACCAGCCA CCATTTTGAA GACAGAGAGC 
AGTCTTACAC CTACAACAGC TGTAAAATGT GAAAAAGAAG TAAACATCAG TCACACTGGA 
GAGCAAGAAA AGACTCAGAG ACCAATAATC ACTGCCCAGG CCCTTCAGAT GGTGCAGTTG 
CGACCTGTTA AGCTCAAGCA AATAGAAACT ATTTTTACAT CTGTCAGTTT TGATCATTCT 
GATCACCAAG CACAAAACCA TTCTGATACA AACTCAGGAA AGTCCCTGGA AATTGATAAT 
ACAGGGCAAA CAGTGACCGT CAATTGTGTT ACTCAGTCAG AAAATAGTAT GGAAACATCT 
GAATGTGAAT ATTCGGTGAA CAGATTCATA GACGATCTCC CTGAGGTATC AACTAGCACT 
CTACCCCCTG ACCTAGATGA ACAATTAAAA AAAACTGAGC AAAATAATCC AGTGCACCTC 
TCTTCCAAGT CCACAAATTC CTTAGCTTCT CCTCTGAGTT CTCCAATGAA ACAGAAGCCT 
CCTATATCAC CCAAAAAACC CAATCTTTCC CTTATTATAC CACCCATAAT GCACTTGCCA 
TTTTCCAACG GACTCAAGCT TCAGCAGGTT TATGAAGCAC AAGAGACACT AGAGTCAGAG 
CAAGTGAATG GTGCATCCTC GACAAACACA GTTAATTTGC AAGCAATTCC TTTAGACCTT 
GAGGTAGAGG AAGAGAGTGA CTCAGACTTA TCAACCCCTG TGGTCAAGAG CAGACTATCT 
TTCTGCAGCG AGTTATCATC AAGCAGTTTC CAAGACCTGC AACTTTCTGA TTACATTCTT 
AATGAGCATG ACCTCGGCCT GAGCGATAAA GACTTTGGGC TCTGTGATGA TAAAAGCACA 
TCAGATGATG GTTCAAGCAG CAGCTCAGGA TCCATCAGCT TTAAAGAGGA TGAACATGAG 
GAAAACGGTG CTGTGTTTGA CTCAATCACT GAAAGCACAT CTGACATTAA TGCTAGCGGA 
GAAGCTGTGG AGGGGATGGT GACGCCTATT CGCCCTCGTA CTACAGAGGA CCTCTTTGCT 
GCAATTCATA GGTCAAAAAG GAAGGTCCTG GGCCGTGGCG ATTCTGAAGA TGAACGCTGC 
CGTAACCTCC TTCCTTCCCC ACCTGTCACT CCAACTGGCT CCTGCCCCAG CTTGCCACGT 
CAGACCAGCT CCATCCAGCG GAACCTACGT CGCTCCACCA CAAGCAATGA CAGTTTTAAA 
GCTTTGCTAC TCAAGAAGGG CAGCCGCTCA GAGTCCAGCT TTCGCATGTC AGCCGCTGAG 
ATTCTCAAAT GCACAGACCC CCGTTTCCAA AGGGCTAATT CAGAAAATGC ACTAGCTGAC 
GGACTGTCCT CTCCGACAAG CAGCAGGAGA GCACATGAAG AGTGGGCACG GACGGAGGGA 
GCTTTGCCTC GCTTTACCAC TGGACAAACT ATCCCAAAAT ATGGGCGCTC GCGCACACCA 
CCTTCCGCAG CCAGCAGCCG GTATAACAGC CGCAGCCGGA TTACCAGTAG GCCAATGACT 
GCCATCTGTG AACGAGATGG AGAGGTGGCT GAATCTACAG ACTGCTACTT AAGTAGCGAG 
AATCATTTTG TCTTGGCTTT ATCCTCCAGC AGCATGGTCT GCGCTCAAGG CAGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158878.1
CDS	265..5004
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158821.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158821.3

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGGCTTCC GATTCACCTA ATGAAGAGGA TAAACTTGTG 
TACTCAATGC CGCCACAAAC CCCTCTGCTG GAGCATGGCT CTGACATGGA CATCCAGAGA 
AGCTGGACCA GCTGTCTACC TTTGCCCACT CCCGAGGAGA AGATGAGACT GCAGGCCCAA 
TCGGTGGCTA CTGATATAAT TCCTATCAAC ATTACAGGTG AGAACTTTGA CCGCCAGGCC 
AGCTTTCGCC GCTCCATGGC AAACACTGAT ACTATTATAC GAAGACCTAA GAGAGTTCAA 
AGAAGAAAGA CAATAACAGG AGTGCCTGAC AACATCCAGA CTGAACTAGC AAATAAAGAA 
CAAGCTGACT CCAGAATACA TTCGAGTATG GCAGATCCAT ACTCCACCCT CAGTCGAGTG 
GGAAGTGTAA ACTCCACCCT GAAACGCTCA GACACCCGTG ATTCCTGTTG TCAAACAGAA 
GAGGTGAAGA TTGTTCCTCC CTCAGTACGC AGAATCAGAG CACAAAGAGG CCATGGAATA 
GCTGCCCAAA TGGCCAGTGT CTCCTCCTCA AGCAGCATTT CCACCATAAG TGACTGTAAT 
GGCATTAGCT ACACACAGCT TAATGACAGT GATCAGAGCT TTCAAAGTTT ACCAAGACAA 
GGTTCTCGCA TCCCGCAACA ACACAGTGAA ACAAAGTACA ATAGCTCCCC CTACAGGGCG 
AATTCTGGTT CTACAAGCCC CTTGTCCTAC CATTTTAACA TGCAGCATGA CTCTTCATCA 
TCGCTGCATA TGCTGTCTGA CTCGAGAAGC AAGCTTACTG AAAATCTAAA TTACGAAGGC 
TCTGATAAAA ATAATACCTC AAGCTTGTTT AGTCTCAGTG GTGCTCTACA GAACTCCATT 
CCTTATGGAT ACAAAAGGAC AGAGGACAAC CAAATGCAAT CACCTCATTC GTCAGTCCAG 
TTGCTCAATT CTGAGGATGC CTCATTGTCG GGTGTCTCCT CGTGTTATGA ATCCACAGCG 
TCCCTCTGTA CAGGTATTCA TACAGAGACT GAGTCCCAGT GCAGCACACT TGATGGCAGA 
AACTGCTGCA GTCCTAACTG TGTCCGCAGG GATTCCAACT TCTCAGAGAG CAGCAACCAA 
AGCTGCAGCA CACTAACCAC GGACCAATGG ACGTATGAAG TTTCTCCAAA AGACAATGTT 
ACTTCAAGTT GCTCCTCACC TGTCAGCCAT ATATACCACG CAGAGGAACA TTCTCCCAGC 
AAGACAGATA CAAGCTCATT GTTCTCTGTT GACAATGAAG GTTACTACAC CTCCATGCGC 
CTAGACTCTG GTTTGAAGTC ACACAGTCAC GGCTGCATCA ATAAAGCAGG AGAACAAAAG 
CACAGTTTGT ATGAGTGCAA AGACCACCAC AGTCAAAGTG ATACTGCAAG TCTGCATAGC 
AACCGCTCCC TGACTCGTAG CATCTCTCTG CGAAAAGCCA GGAAGCCCCC ACTCCCTCCA 
GAAAGAACCG ACTCTTTAAG GCGTAAACCC CAGAGGAAGA GTCGCCACAG TGGTTCAGTC 
CTTAATGAGC AGTTAATCTC TAGTCTCCAG CAGTCCTTAC AACTTAACCT AAATACTCAA 
ACTACATCTT GCTCTGAACA GTCACCCTGT GGTAGGTTTG AAGACCCTTG GGTCCTCCGA 
CCGAGAAGTC AGAGTATCAT AAGTGCAGCT AGCAGTGGGG TATCTGCACC AGCTGCTGTA 
TGTCCGGTCA CACCAACACA CAGTGACAGC AGCAGTCAGC GCTCAGACTA TGCAGAGTCA 
TGGGACTTCT ACATGGATTG CCCATGTACA CGGTCTGACC AGAGTCTTTC CTCACAAATC 
ACAAGGTCTG CAAGTGCTGA GGAGAATCAT GGAGTCATAG GTAATGCATC CTACAACAGT 
GAATTCCCAT CATCCCCCTT CAGCAGTAGT CAGTCAAAAT TGACCTCATC TCCTGACAAA 
GTCCATCGAC TCACGTCACC ATCAAGTGGC TACTCAAGTC AGTCTAACAC TCCAACTGCA 
GGCACTCCAG TTACGTCTCT AATAAGAGCA AAGTCTCCTG CGGGGAGACC GAAACCAAAA 
GTGCCAGAAA GAAAGTCCTC TCTACGATCT TCTATATCAT CCTCTTCTAC ATCCCTGTCC 
TCCAACACCT CAGATTCCAT CAGGAACATT CCTCCACCTC CACCTTTACC TGACATGACA 
ACTGCATTAT GTGGGTCGCC CAGTTCTGCT TCTTTCTCTC CAACAGCGGC TAAAATAGAC 
CCAATACCCT CTTTTACATC TCTACAGGAT GTATCATCTG CCTTGGAAGA TGGGCAAAAT 
ACACTTTCAG ATACATCTGC AACACCTGAG GAATGTGATA GTCCTGATTT TCCCCCTCCC 
CATTCAAATG TTTTTGAATC TCTGTCAGGC AATGTAGATA CCAACAGCCT CCCACCTCCT 
CCACTTCTGC TTTGCAAACC TTCACTGTGC ATTTCATCTC TTCTTCCCCC TCCTCCTCCA 
CCTCCTCCAC TATTACTTGA GACCAGTCTT CAACCAGCCA CCATTTTGAA GACAGAGAGC 
AGTCTTACAC CTACAACAGC TGTAAAATGT GAAAAAGAAG TAAACATCAG TCACACTGGA 
GAGCAAGAAA AGACTCAGAG ACCAATAATC ACTGCCCAGG CCCTTCAGAT GGTGCAGTTG 
CGACCTGTTA AGCTCAAGCA AATAGAAACT ATTTTTACAT CTGTCAGTTT TGATCATTCT 
GATCACCAAG CACAAAACCA TTCTGATACA AACTCAGGAA AGTCCCTGGA AATTGATAAT 
ACAGGGCAAA CAGTGACCGT CAATTGTGTT ACTCAGTCAG AAAATAGTAT GGAAACATCT 
GAATGTGAAT ATTCGGTGAA CAGATTCATA GACGATCTCC CTGAGGTATC AACTAGCACT 
CTACCCCCTG ACCTAGATGA ACAATTAAAA AAAACTGAGC AAAATAATCC AGTGCACCTC 
TCTTCCAAGT CCACAAATTC CTTAGCTTCT CCTCTGAGTT CTCCAATGAA ACAGAAGCCT 
CCTATATCAC CCAAAAAACC CAATCTTTCC CTTATTATAC CACCCATAAT GCACTTGCCA 
TTTTCCAACG GACTCAAGCT TCAGCAGGTT TATGAAGCAC AAGAGACACT AGAGTCAGAG 
CAAGTGAATG GTGCATCCTC GACAAACACA GTTAATTTGC AAGCAATTCC TTTAGACCTT 
GAGGTAGAGG AAGAGAGTGA CTCAGACTTA TCAACCCCTG TGGTCAAGAG CAGACTATCT 
TTCTGCAGCG AGTTATCATC AAGCAGTTTC CAAGACCTGC AACTTTCTGA TTACATTCTT 
AATGAGCATG ACCTCGGCCT GAGCGATAAA GACTTTGGGC TCTGTGATGA TAAAAGCACA 
TCAGATGATG GTTCAAGCAG CAGCTCAGGA TCCATCAGCT TTAAAGAGGA TGAACATGAG 
GAAAACGGTG CTGTGTTTGA CTCAATCACT GAAAGCACAT CTGACATTAA TGCTAGCGGA 
GAAGCTGTGG AGGGGATGGT GACGCCTATT CGCCCTCGTA CTACAGAGGA CCTCTTTGCT 
GCAATTCATA GGTCAAAAAG GAAGGTCCTG GGCCGTGGCG ATTCTGAAGA TGAACGCTGC 
CGTAACCTCC TTCCTTCCCC ACCTGTCACT CCAACTGGCT CCTGCCCCAG CTTGCCACGT 
CAGACCAGCT CCATCCAGCG GAACCTACGT CGCTCCACCA CAAGCAATGA CAGTTTTAAA 
GCTTTGCTAC TCAAGAAGGG CAGCCGCTCA GAGTCCAGCT TTCGCATGTC AGCCGCTGAG 
ATTCTCAAAT GCACAGACCC CCGTTTCCAA AGGGCTAATT CAGAAAATGC ACTAGCTGAC 
GGACTGTCCT CTCCGACAAG CAGCAGGAGA GCACATGAAG AGTGGGCACG GACGGAGGGA 
GCTTTGCCTC GCTTTACCAC TGGACAAACT ATCCCAAAAT ATGGGCGCTC GCGCACACCA 
CCTTCCGCAG CCAGCAGCCG GTATAACAGC CGCAGCCGGA TTACCAGTAG GCCAATGACT 
GCCATCTGTG AACGAGATGG AGAGGTGGCT GAATCTACAG ACTGCTACTT AAGTAGCGAG 
AATCATTTTG TCTTGGCTTT ATCCTCCAGC AGCATGGTCT GCGCTCAAGG CAGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467437.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323112.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.

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CloneID	ODa22651
Clone ID Related Accession (Same CDS sequence)	XM_005158821.3 , XM_021467437.1
Accession Version	XM_021467437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGGCTTCC GATTCACCTA ATGAAGAGGA TAAACTTGTG 
TACTCAATGC CGCCACAAAC CCCTCTGCTG GAGCATGGCT CTGACATGGA CATCCAGAGA 
AGCTGGACCA GCTGTCTACC TTTGCCCACT CCCGAGGAGA AGATGAGACT GCAGGCCCAA 
TCGGTGGCTA CTGATATAAT TCCTATCAAC ATTACAGGTG AGAACTTTGA CCGCCAGGCC 
AGCTTTCGCC GCTCCATGGC AAACACTGAT ACTATTATAC GAAGACCTAA GAGAGTTCAA 
AGAAGAAAGA CAATAACAGG AGTGCCTGAC AACATCCAGA CTGAACTAGC AAATAAAGAA 
CAAGCTGACT CCAGAATACA TTCGAGTATG GCAGATCCAT ACTCCACCCT CAGTCGAGTG 
GGAAGTGTAA ACTCCACCCT GAAACGCTCA GACACCCGTG ATTCCTGTTG TCAAACAGAA 
GAGGTGAAGA TTGTTCCTCC CTCAGTACGC AGAATCAGAG CACAAAGAGG CCATGGAATA 
GCTGCCCAAA TGGCCAGTGT CTCCTCCTCA AGCAGCATTT CCACCATAAG TGACTGTAAT 
GGCATTAGCT ACACACAGCT TAATGACAGT GATCAGAGCT TTCAAAGTTT ACCAAGACAA 
GGTTCTCGCA TCCCGCAACA ACACAGTGAA ACAAAGTACA ATAGCTCCCC CTACAGGGCG 
AATTCTGGTT CTACAAGCCC CTTGTCCTAC CATTTTAACA TGCAGCATGA CTCTTCATCA 
TCGCTGCATA TGCTGTCTGA CTCGAGAAGC AAGCTTACTG AAAATCTAAA TTACGAAGGC 
TCTGATAAAA ATAATACCTC AAGCTTGTTT AGTCTCAGTG GTGCTCTACA GAACTCCATT 
CCTTATGGAT ACAAAAGGAC AGAGGACAAC CAAATGCAAT CACCTCATTC GTCAGTCCAG 
TTGCTCAATT CTGAGGATGC CTCATTGTCG GGTGTCTCCT CGTGTTATGA ATCCACAGCG 
TCCCTCTGTA CAGGTATTCA TACAGAGACT GAGTCCCAGT GCAGCACACT TGATGGCAGA 
AACTGCTGCA GTCCTAACTG TGTCCGCAGG GATTCCAACT TCTCAGAGAG CAGCAACCAA 
AGCTGCAGCA CACTAACCAC GGACCAATGG ACGTATGAAG TTTCTCCAAA AGACAATGTT 
ACTTCAAGTT GCTCCTCACC TGTCAGCCAT ATATACCACG CAGAGGAACA TTCTCCCAGC 
AAGACAGATA CAAGCTCATT GTTCTCTGTT GACAATGAAG GTTACTACAC CTCCATGCGC 
CTAGACTCTG GTTTGAAGTC ACACAGTCAC GGCTGCATCA ATAAAGCAGG AGAACAAAAG 
CACAGTTTGT ATGAGTGCAA AGACCACCAC AGTCAAAGTG ATACTGCAAG TCTGCATAGC 
AACCGCTCCC TGACTCGTAG CATCTCTCTG CGAAAAGCCA GGAAGCCCCC ACTCCCTCCA 
GAAAGAACCG ACTCTTTAAG GCGTAAACCC CAGAGGAAGA GTCGCCACAG TGGTTCAGTC 
CTTAATGAGC AGTTAATCTC TAGTCTCCAG CAGTCCTTAC AACTTAACCT AAATACTCAA 
ACTACATCTT GCTCTGAACA GTCACCCTGT GGTAGGTTTG AAGACCCTTG GGTCCTCCGA 
CCGAGAAGTC AGAGTATCAT AAGTGCAGCT AGCAGTGGGG TATCTGCACC AGCTGCTGTA 
TGTCCGGTCA CACCAACACA CAGTGACAGC AGCAGTCAGC GCTCAGACTA TGCAGAGTCA 
TGGGACTTCT ACATGGATTG CCCATGTACA CGGTCTGACC AGAGTCTTTC CTCACAAATC 
ACAAGGTCTG CAAGTGCTGA GGAGAATCAT GGAGTCATAG GTAATGCATC CTACAACAGT 
GAATTCCCAT CATCCCCCTT CAGCAGTAGT CAGTCAAAAT TGACCTCATC TCCTGACAAA 
GTCCATCGAC TCACGTCACC ATCAAGTGGC TACTCAAGTC AGTCTAACAC TCCAACTGCA 
GGCACTCCAG TTACGTCTCT AATAAGAGCA AAGTCTCCTG CGGGGAGACC GAAACCAAAA 
GTGCCAGAAA GAAAGTCCTC TCTACGATCT TCTATATCAT CCTCTTCTAC ATCCCTGTCC 
TCCAACACCT CAGATTCCAT CAGGAACATT CCTCCACCTC CACCTTTACC TGACATGACA 
ACTGCATTAT GTGGGTCGCC CAGTTCTGCT TCTTTCTCTC CAACAGCGGC TAAAATAGAC 
CCAATACCCT CTTTTACATC TCTACAGGAT GTATCATCTG CCTTGGAAGA TGGGCAAAAT 
ACACTTTCAG ATACATCTGC AACACCTGAG GAATGTGATA GTCCTGATTT TCCCCCTCCC 
CATTCAAATG TTTTTGAATC TCTGTCAGGC AATGTAGATA CCAACAGCCT CCCACCTCCT 
CCACTTCTGC TTTGCAAACC TTCACTGTGC ATTTCATCTC TTCTTCCCCC TCCTCCTCCA 
CCTCCTCCAC TATTACTTGA GACCAGTCTT CAACCAGCCA CCATTTTGAA GACAGAGAGC 
AGTCTTACAC CTACAACAGC TGTAAAATGT GAAAAAGAAG TAAACATCAG TCACACTGGA 
GAGCAAGAAA AGACTCAGAG ACCAATAATC ACTGCCCAGG CCCTTCAGAT GGTGCAGTTG 
CGACCTGTTA AGCTCAAGCA AATAGAAACT ATTTTTACAT CTGTCAGTTT TGATCATTCT 
GATCACCAAG CACAAAACCA TTCTGATACA AACTCAGGAA AGTCCCTGGA AATTGATAAT 
ACAGGGCAAA CAGTGACCGT CAATTGTGTT ACTCAGTCAG AAAATAGTAT GGAAACATCT 
GAATGTGAAT ATTCGGTGAA CAGATTCATA GACGATCTCC CTGAGGTATC AACTAGCACT 
CTACCCCCTG ACCTAGATGA ACAATTAAAA AAAACTGAGC AAAATAATCC AGTGCACCTC 
TCTTCCAAGT CCACAAATTC CTTAGCTTCT CCTCTGAGTT CTCCAATGAA ACAGAAGCCT 
CCTATATCAC CCAAAAAACC CAATCTTTCC CTTATTATAC CACCCATAAT GCACTTGCCA 
TTTTCCAACG GACTCAAGCT TCAGCAGGTT TATGAAGCAC AAGAGACACT AGAGTCAGAG 
CAAGTGAATG GTGCATCCTC GACAAACACA GTTAATTTGC AAGCAATTCC TTTAGACCTT 
GAGGTAGAGG AAGAGAGTGA CTCAGACTTA TCAACCCCTG TGGTCAAGAG CAGACTATCT 
TTCTGCAGCG AGTTATCATC AAGCAGTTTC CAAGACCTGC AACTTTCTGA TTACATTCTT 
AATGAGCATG ACCTCGGCCT GAGCGATAAA GACTTTGGGC TCTGTGATGA TAAAAGCACA 
TCAGATGATG GTTCAAGCAG CAGCTCAGGA TCCATCAGCT TTAAAGAGGA TGAACATGAG 
GAAAACGGTG CTGTGTTTGA CTCAATCACT GAAAGCACAT CTGACATTAA TGCTAGCGGA 
GAAGCTGTGG AGGGGATGGT GACGCCTATT CGCCCTCGTA CTACAGAGGA CCTCTTTGCT 
GCAATTCATA GGTCAAAAAG GAAGGTCCTG GGCCGTGGCG ATTCTGAAGA TGAACGCTGC 
CGTAACCTCC TTCCTTCCCC ACCTGTCACT CCAACTGGCT CCTGCCCCAG CTTGCCACGT 
CAGACCAGCT CCATCCAGCG GAACCTACGT CGCTCCACCA CAAGCAATGA CAGTTTTAAA 
GCTTTGCTAC TCAAGAAGGG CAGCCGCTCA GAGTCCAGCT TTCGCATGTC AGCCGCTGAG 
ATTCTCAAAT GCACAGACCC CCGTTTCCAA AGGGCTAATT CAGAAAATGC ACTAGCTGAC 
GGACTGTCCT CTCCGACAAG CAGCAGGAGA GCACATGAAG AGTGGGCACG GACGGAGGGA 
GCTTTGCCTC GCTTTACCAC TGGACAAACT ATCCCAAAAT ATGGGCGCTC GCGCACACCA 
CCTTCCGCAG CCAGCAGCCG GTATAACAGC CGCAGCCGGA TTACCAGTAG GCCAATGACT 
GCCATCTGTG AACGAGATGG AGAGGTGGCT GAATCTACAG ACTGCTACTT AAGTAGCGAG 
AATCATTTTG TCTTGGCTTT ATCCTCCAGC AGCATGGTCT GCGCTCAAGG CAGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323112.1
CDS	284..5023
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467437.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467437.1

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGGCTTCC GATTCACCTA ATGAAGAGGA TAAACTTGTG 
TACTCAATGC CGCCACAAAC CCCTCTGCTG GAGCATGGCT CTGACATGGA CATCCAGAGA 
AGCTGGACCA GCTGTCTACC TTTGCCCACT CCCGAGGAGA AGATGAGACT GCAGGCCCAA 
TCGGTGGCTA CTGATATAAT TCCTATCAAC ATTACAGGTG AGAACTTTGA CCGCCAGGCC 
AGCTTTCGCC GCTCCATGGC AAACACTGAT ACTATTATAC GAAGACCTAA GAGAGTTCAA 
AGAAGAAAGA CAATAACAGG AGTGCCTGAC AACATCCAGA CTGAACTAGC AAATAAAGAA 
CAAGCTGACT CCAGAATACA TTCGAGTATG GCAGATCCAT ACTCCACCCT CAGTCGAGTG 
GGAAGTGTAA ACTCCACCCT GAAACGCTCA GACACCCGTG ATTCCTGTTG TCAAACAGAA 
GAGGTGAAGA TTGTTCCTCC CTCAGTACGC AGAATCAGAG CACAAAGAGG CCATGGAATA 
GCTGCCCAAA TGGCCAGTGT CTCCTCCTCA AGCAGCATTT CCACCATAAG TGACTGTAAT 
GGCATTAGCT ACACACAGCT TAATGACAGT GATCAGAGCT TTCAAAGTTT ACCAAGACAA 
GGTTCTCGCA TCCCGCAACA ACACAGTGAA ACAAAGTACA ATAGCTCCCC CTACAGGGCG 
AATTCTGGTT CTACAAGCCC CTTGTCCTAC CATTTTAACA TGCAGCATGA CTCTTCATCA 
TCGCTGCATA TGCTGTCTGA CTCGAGAAGC AAGCTTACTG AAAATCTAAA TTACGAAGGC 
TCTGATAAAA ATAATACCTC AAGCTTGTTT AGTCTCAGTG GTGCTCTACA GAACTCCATT 
CCTTATGGAT ACAAAAGGAC AGAGGACAAC CAAATGCAAT CACCTCATTC GTCAGTCCAG 
TTGCTCAATT CTGAGGATGC CTCATTGTCG GGTGTCTCCT CGTGTTATGA ATCCACAGCG 
TCCCTCTGTA CAGGTATTCA TACAGAGACT GAGTCCCAGT GCAGCACACT TGATGGCAGA 
AACTGCTGCA GTCCTAACTG TGTCCGCAGG GATTCCAACT TCTCAGAGAG CAGCAACCAA 
AGCTGCAGCA CACTAACCAC GGACCAATGG ACGTATGAAG TTTCTCCAAA AGACAATGTT 
ACTTCAAGTT GCTCCTCACC TGTCAGCCAT ATATACCACG CAGAGGAACA TTCTCCCAGC 
AAGACAGATA CAAGCTCATT GTTCTCTGTT GACAATGAAG GTTACTACAC CTCCATGCGC 
CTAGACTCTG GTTTGAAGTC ACACAGTCAC GGCTGCATCA ATAAAGCAGG AGAACAAAAG 
CACAGTTTGT ATGAGTGCAA AGACCACCAC AGTCAAAGTG ATACTGCAAG TCTGCATAGC 
AACCGCTCCC TGACTCGTAG CATCTCTCTG CGAAAAGCCA GGAAGCCCCC ACTCCCTCCA 
GAAAGAACCG ACTCTTTAAG GCGTAAACCC CAGAGGAAGA GTCGCCACAG TGGTTCAGTC 
CTTAATGAGC AGTTAATCTC TAGTCTCCAG CAGTCCTTAC AACTTAACCT AAATACTCAA 
ACTACATCTT GCTCTGAACA GTCACCCTGT GGTAGGTTTG AAGACCCTTG GGTCCTCCGA 
CCGAGAAGTC AGAGTATCAT AAGTGCAGCT AGCAGTGGGG TATCTGCACC AGCTGCTGTA 
TGTCCGGTCA CACCAACACA CAGTGACAGC AGCAGTCAGC GCTCAGACTA TGCAGAGTCA 
TGGGACTTCT ACATGGATTG CCCATGTACA CGGTCTGACC AGAGTCTTTC CTCACAAATC 
ACAAGGTCTG CAAGTGCTGA GGAGAATCAT GGAGTCATAG GTAATGCATC CTACAACAGT 
GAATTCCCAT CATCCCCCTT CAGCAGTAGT CAGTCAAAAT TGACCTCATC TCCTGACAAA 
GTCCATCGAC TCACGTCACC ATCAAGTGGC TACTCAAGTC AGTCTAACAC TCCAACTGCA 
GGCACTCCAG TTACGTCTCT AATAAGAGCA AAGTCTCCTG CGGGGAGACC GAAACCAAAA 
GTGCCAGAAA GAAAGTCCTC TCTACGATCT TCTATATCAT CCTCTTCTAC ATCCCTGTCC 
TCCAACACCT CAGATTCCAT CAGGAACATT CCTCCACCTC CACCTTTACC TGACATGACA 
ACTGCATTAT GTGGGTCGCC CAGTTCTGCT TCTTTCTCTC CAACAGCGGC TAAAATAGAC 
CCAATACCCT CTTTTACATC TCTACAGGAT GTATCATCTG CCTTGGAAGA TGGGCAAAAT 
ACACTTTCAG ATACATCTGC AACACCTGAG GAATGTGATA GTCCTGATTT TCCCCCTCCC 
CATTCAAATG TTTTTGAATC TCTGTCAGGC AATGTAGATA CCAACAGCCT CCCACCTCCT 
CCACTTCTGC TTTGCAAACC TTCACTGTGC ATTTCATCTC TTCTTCCCCC TCCTCCTCCA 
CCTCCTCCAC TATTACTTGA GACCAGTCTT CAACCAGCCA CCATTTTGAA GACAGAGAGC 
AGTCTTACAC CTACAACAGC TGTAAAATGT GAAAAAGAAG TAAACATCAG TCACACTGGA 
GAGCAAGAAA AGACTCAGAG ACCAATAATC ACTGCCCAGG CCCTTCAGAT GGTGCAGTTG 
CGACCTGTTA AGCTCAAGCA AATAGAAACT ATTTTTACAT CTGTCAGTTT TGATCATTCT 
GATCACCAAG CACAAAACCA TTCTGATACA AACTCAGGAA AGTCCCTGGA AATTGATAAT 
ACAGGGCAAA CAGTGACCGT CAATTGTGTT ACTCAGTCAG AAAATAGTAT GGAAACATCT 
GAATGTGAAT ATTCGGTGAA CAGATTCATA GACGATCTCC CTGAGGTATC AACTAGCACT 
CTACCCCCTG ACCTAGATGA ACAATTAAAA AAAACTGAGC AAAATAATCC AGTGCACCTC 
TCTTCCAAGT CCACAAATTC CTTAGCTTCT CCTCTGAGTT CTCCAATGAA ACAGAAGCCT 
CCTATATCAC CCAAAAAACC CAATCTTTCC CTTATTATAC CACCCATAAT GCACTTGCCA 
TTTTCCAACG GACTCAAGCT TCAGCAGGTT TATGAAGCAC AAGAGACACT AGAGTCAGAG 
CAAGTGAATG GTGCATCCTC GACAAACACA GTTAATTTGC AAGCAATTCC TTTAGACCTT 
GAGGTAGAGG AAGAGAGTGA CTCAGACTTA TCAACCCCTG TGGTCAAGAG CAGACTATCT 
TTCTGCAGCG AGTTATCATC AAGCAGTTTC CAAGACCTGC AACTTTCTGA TTACATTCTT 
AATGAGCATG ACCTCGGCCT GAGCGATAAA GACTTTGGGC TCTGTGATGA TAAAAGCACA 
TCAGATGATG GTTCAAGCAG CAGCTCAGGA TCCATCAGCT TTAAAGAGGA TGAACATGAG 
GAAAACGGTG CTGTGTTTGA CTCAATCACT GAAAGCACAT CTGACATTAA TGCTAGCGGA 
GAAGCTGTGG AGGGGATGGT GACGCCTATT CGCCCTCGTA CTACAGAGGA CCTCTTTGCT 
GCAATTCATA GGTCAAAAAG GAAGGTCCTG GGCCGTGGCG ATTCTGAAGA TGAACGCTGC 
CGTAACCTCC TTCCTTCCCC ACCTGTCACT CCAACTGGCT CCTGCCCCAG CTTGCCACGT 
CAGACCAGCT CCATCCAGCG GAACCTACGT CGCTCCACCA CAAGCAATGA CAGTTTTAAA 
GCTTTGCTAC TCAAGAAGGG CAGCCGCTCA GAGTCCAGCT TTCGCATGTC AGCCGCTGAG 
ATTCTCAAAT GCACAGACCC CCGTTTCCAA AGGGCTAATT CAGAAAATGC ACTAGCTGAC 
GGACTGTCCT CTCCGACAAG CAGCAGGAGA GCACATGAAG AGTGGGCACG GACGGAGGGA 
GCTTTGCCTC GCTTTACCAC TGGACAAACT ATCCCAAAAT ATGGGCGCTC GCGCACACCA 
CCTTCCGCAG CCAGCAGCCG GTATAACAGC CGCAGCCGGA TTACCAGTAG GCCAATGACT 
GCCATCTGTG AACGAGATGG AGAGGTGGCT GAATCTACAG ACTGCTACTT AAGTAGCGAG 
AATCATTTTG TCTTGGCTTT ATCCTCCAGC AGCATGGTCT GCGCTCAAGG CAGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158822.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158879.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.

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CloneID	ODa22652
Clone ID Related Accession (Same CDS sequence)	XM_005158822.3 , XM_021467439.1
Accession Version	XM_005158822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGT GTGGGACGAA GTTGGGGGGG GCAGTTGCTC CGGCTGGGGC GCTGGGCTGT 
GTGAGGACAG TGCGCTGCAA AACTCTTTTG ACACACTTCC CAGCAAATAC CAACACACAG 
CGGGAGAGGC AGGAGACCAT GTTCTGTCTG AGAGCGGTGT CCAGTCTTGA TGAGGAGAGC 
AAATGGTCGG TGCACTACAC AGCCCCCTGG CACCACCAAG AGAACGTCTT CCTGCCTGGG 
TCCAGACCAG CATGTGTTGA GGATCTTCAC CGTCAGGCCA AAGTCAACCT GAAAACAGTC 
CTCAGAGAGT GTGACAAGCT CAGAAAAGAT GGCTTTCGCA GTTCTCAGTA TTACTCCCAA 
AGCCCAGCAT TCTCTGCAAC CAGCCTTTGT GAAAGTGTAC ATCTGGATGA AGAGAAGACA 
AAGAAAAAGA AGCGGTTTAA AGTCCTAGAC TGCCTATCAC AGTCTTGCCT CTCTCTCTGC 
TGTCATTTGA GATCTTGGAA AACAAAGGCT TCCGATTCAC CTAATGAAGA GGATAAACTT 
GTGTACTCAA TGCCGCCACA AACCCCTCTG CTGGAGCATG GCTCTGACAT GGACATCCAG 
AGAAGCTGGA CCAGCTGTCT ACCTTTGCCC ACTCCCGAGG AGAAGATGAG ACTGCAGGCC 
CAATCGGTGG CTACTGATAT AATTCCTATC AACATTACAG GTGAGAACTT TGACCGCCAG 
GCCAGCTTTC GCCGCTCCAT GGCAAACACT GATACTATTA TACGAAGACC TAAGAGAGTT 
CAAAGAAGAA AGACAATAAC AGGAGTGCCT GACAACATCC AGACTGAACT AGCAAATAAA 
GAACAAGCTG ACTCCAGAAT ACATTCGAGT ATGGCAGATC CATACTCCAC CCTCAGTCGA 
GTGGGAAGTG TAAACTCCAC CCTGAAACGC TCAGACACCC GTGATTCCTG TTGTCAAACA 
GAAGAGGTGA AGATTGTTCC TCCCTCAGTA CGCAGAATCA GAGCACAAAG AGGCCATGGA 
ATAGCTGCCC AAATGGCCAG TGTCTCCTCC TCAAGCAGCA TTTCCACCAT AAGTGACTGT 
AATGGCATTA GCTACACACA GCTTAATGAC AGTGATCAGA GCTTTCAAAG TTTACCAAGA 
CAAGGTTCTC GCATCCCGCA ACAACACAGT GAAACAAAGT ACAATAGCTC CCCCTACAGG 
GCGAATTCTG GTTCTACAAG CCCCTTGTCC TACCATTTTA ACATGCAGCA TGACTCTTCA 
TCATCGCTGC ATATGCTGTC TGACTCGAGA AGCAAGCTTA CTGAAAATCT AAATTACGAA 
GGCTCTGATA AAAATAATAC CTCAAGCTTG TTTAGTCTCA GTGGTGCTCT ACAGAACTCC 
ATTCCTTATG GATACAAAAG GACAGAGGAC AACCAAATGC AATCACCTCA TTCGTCAGTC 
CAGTTGCTCA ATTCTGAGGA TGCCTCATTG TCGGGTGTCT CCTCGTGTTA TGAATCCACA 
GCGTCCCTCT GTACAGGTAT TCATACAGAG ACTGAGTCCC AGTGCAGCAC ACTTGATGGC 
AGAAACTGCT GCAGTCCTAA CTGTGTCCGC AGGGATTCCA ACTTCTCAGA GAGCAGCAAC 
CAAAGCTGCA GCACACTAAC CACGGACCAA TGGACGTATG AAGTTTCTCC AAAAGACAAT 
GTTACTTCAA GTTGCTCCTC ACCTGTCAGC CATATATACC ACGCAGAGGA ACATTCTCCC 
AGCAAGACAG ATACAAGCTC ATTGTTCTCT GTTGACAATG AAGGTTACTA CACCTCCATG 
CGCCTAGACT CTGGTTTGAA GTCACACAGT CACGGCTGCA TCAATAAAGC AGGAGAACAA 
AAGCACAGTT TGTATGAGTG CAAAGACCAC CACAGTCAAA GTGATACTGC AAGTCTGCAT 
AGCAACCGCT CCCTGACTCG TAGCATCTCT CTGCGAAAAG CCAGGAAGCC CCCACTCCCT 
CCAGAAAGAA CCGACTCTTT AAGGCGTAAA CCCCAGAGGA AGAGTCGCCA CAGTGGTTCA 
GTCCTTAATG AGCAGTTAAT CTCTAGTCTC CAGCAGTCCT TACAACTTAA CCTAAATACT 
CAAACTACAT CTTGCTCTGA ACAGTCACCC TGTGGTAGGT TTGAAGACCC TTGGGTCCTC 
CGACCGAGAA GTCAGAGTAT CATAAGTGCA GCTAGCAGTG GGGTATCTGC ACCAGCTGCT 
GTATGTCCGG TCACACCAAC ACACAGTGAC AGCAGCAGTC AGCGCTCAGA CTATGCAGAG 
TCATGGGACT TCTACATGGA TTGCCCATGT ACACGGTCTG ACCAGAGTCT TTCCTCACAA 
ATCACAAGGT CTGCAAGTGC TGAGGAGAAT CATGGAGTCA TAGGTAATGC ATCCTACAAC 
AGTGAATTCC CATCATCCCC CTTCAGCAGT AGTCAGTCAA AATTGACCTC ATCTCCTGAC 
AAAGTCCATC GACTCACGTC ACCATCAAGT GGCTACTCAA GTCAGTCTAA CACTCCAACT 
GCAGGCACTC CAGTTACGTC TCTAATAAGA GCAAAGTCTC CTGCGGGGAG ACCGAAACCA 
AAAGTGCCAG AAAGAAAGTC CTCTCTACGA TCTTCTATAT CATCCTCTTC TACATCCCTG 
TCCTCCAACA CCTCAGATTC CATCAGGAAC ATTCCTCCAC CTCCACCTTT ACCTGACATG 
ACAACTGCAT TATGTGGGTC GCCCAGTTCT GCTTCTTTCT CTCCAACAGC GGCTAAAATA 
GACCCAATAC CCTCTTTTAC ATCTCTACAG GATGTATCAT CTGCCTTGGA AGATGGGCAA 
AATACACTTT CAGATACATC TGCAACACCT GAGGAATGTG ATAGTCCTGA TTTTCCCCCT 
CCCCATTCAA ATGTTTTTGA ATCTCTGTCA GGCAATGTAG ATACCAACAG CCTCCCACCT 
CCTCCACTTC TGCTTTGCAA ACCTTCACTG TGCATTTCAT CTCTTCTTCC CCCTCCTCCT 
CCACCTCCTC CACTATTACT TGAGACCAGT CTTCAACCAG CCACCATTTT GAAGACAGAG 
AGCAGTCTTA CACCTACAAC AGCTGTAAAA TGTGAAAAAG AAGTAAACAT CAGTCACACT 
GGAGAGCAAG AAAAGACTCA GAGACCAATA ATCACTGCCC AGGCCCTTCA GATGGTGCAG 
TTGCGACCTG TTAAGCTCAA GCAAATAGAA ACTATTTTTA CATCTGTCAG TTTTGATCAT 
TCTGATCACC AAGCACAAAA CCATTCTGAT ACAAACTCAG GAAAGTCCCT GGAAATTGAT 
AATACAGGGC AAACAGTGAC CGTCAATTGT GTTACTCAGT CAGAAAATAG TATGGAAACA 
TCTGAATGTG AATATTCGGT GAACAGATTC ATAGACGATC TCCCTGAGGT ATCAACTAGC 
ACTCTACCCC CTGACCTAGA TGAACAATTA AAAAAAACTG AGCAAAATAA TCCAGTGCAC 
CTCTCTTCCA AGTCCACAAA TTCCTTAGCT TCTCCTCTGA GTTCTCCAAT GAAACAGAAG 
CCTCCTATAT CACCCAAAAA ACCCAATCTT TCCCTTATTA TACCACCCAT AATGCACTTG 
CCATTTTCCA ACGGACTCAA GCTTCAGCAG GTTTATGAAG CACAAGAGAC ACTAGAGTCA 
GAGCAAGTGA ATGGTGCATC CTCGACAAAC ACAGTTAATT TGCAAGCAAT TCCTTTAGAC 
CTTGAGGTAG AGGAAGAGAG TGACTCAGAC TTATCAACCC CTGTGGTCAA GAGCAGACTA 
TCTTTCTGCA GCGAGTTATC ATCAAGCAGT TTCCAAGACC TGCAACTTTC TGATTACATT 
CTTAATGAGC ATGACCTCGG CCTGAGCGAT AAAGACTTTG GGCTCTGTGA TGATAAAAGC 
ACATCAGATG ATGGTTCAAG CAGCAGCTCA GGATCCATCA GCTTTAAAGA GGATGAACAT 
GAGGAAAACG GTGCTGTGTT TGACTCAATC ACTGAAAGCA CATCTGACAT TAATGCTAGC 
GGAGAAGCTG TGGAGGGGAT GGTGACGCCT ATTCGCCCTC GTACTACAGA GGACCTCTTT 
GCTGCAATTC ATAGGTCAAA AAGGAAGGTC CTGGGCCGTG GCGATTCTGA AGATGAACGC 
TGCCGTAACC TCCTTCCTTC CCCACCTGTC ACTCCAACTG GCTCCTGCCC CAGCTTGCCA 
CGTCAGACCA GCTCCATCCA GCGGAACCTA CGTCGCTCCA CCACAAGCAA TGACAGTTTT 
AAAGCTTTGC TACTCAAGAA GGGCAGCCGC TCAGAGTCCA GCTTTCGCAT GTCAGCCGCT 
GAGATTCTCA AATGCACAGA CCCCCGTTTC CAAAGGGCTA ATTCAGAAAA TGCACTAGCT 
GACGGACTGT CCTCTCCGAC AAGCAGCAGG AGAGCACATG AAGAGTGGGC ACGGACGGAG 
GGAGCTTTGC CTCGCTTTAC CACTGGACAA ACTATCCCAA AATATGGGCG CTCGCGCACA 
CCACCTTCCG CAGCCAGCAG CCGGTATAAC AGCCGCAGCC GGATTACCAG TAGGCCAATG 
ACTGCCATCT GTGAACGAGA TGGAGAGGTG GCTGAATCTA CAGACTGCTA CTTAAGTAGC 
GAGAATCATT TTGTCTTGGC TTTATCCTCC AGCAGCATGG TCTGCGCTCA AGGCAGCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158879.1
CDS	182..4864
Translation	MNLCGTKLGGAVAPAGALGCVRTVRCKTLLTHFPANTNTQRERQETMFCLRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158822.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158822.3

ORF Insert Sequence:

ATGAACCTGT GTGGGACGAA GTTGGGGGGG GCAGTTGCTC CGGCTGGGGC GCTGGGCTGT 
GTGAGGACAG TGCGCTGCAA AACTCTTTTG ACACACTTCC CAGCAAATAC CAACACACAG 
CGGGAGAGGC AGGAGACCAT GTTCTGTCTG AGAGCGGTGT CCAGTCTTGA TGAGGAGAGC 
AAATGGTCGG TGCACTACAC AGCCCCCTGG CACCACCAAG AGAACGTCTT CCTGCCTGGG 
TCCAGACCAG CATGTGTTGA GGATCTTCAC CGTCAGGCCA AAGTCAACCT GAAAACAGTC 
CTCAGAGAGT GTGACAAGCT CAGAAAAGAT GGCTTTCGCA GTTCTCAGTA TTACTCCCAA 
AGCCCAGCAT TCTCTGCAAC CAGCCTTTGT GAAAGTGTAC ATCTGGATGA AGAGAAGACA 
AAGAAAAAGA AGCGGTTTAA AGTCCTAGAC TGCCTATCAC AGTCTTGCCT CTCTCTCTGC 
TGTCATTTGA GATCTTGGAA AACAAAGGCT TCCGATTCAC CTAATGAAGA GGATAAACTT 
GTGTACTCAA TGCCGCCACA AACCCCTCTG CTGGAGCATG GCTCTGACAT GGACATCCAG 
AGAAGCTGGA CCAGCTGTCT ACCTTTGCCC ACTCCCGAGG AGAAGATGAG ACTGCAGGCC 
CAATCGGTGG CTACTGATAT AATTCCTATC AACATTACAG GTGAGAACTT TGACCGCCAG 
GCCAGCTTTC GCCGCTCCAT GGCAAACACT GATACTATTA TACGAAGACC TAAGAGAGTT 
CAAAGAAGAA AGACAATAAC AGGAGTGCCT GACAACATCC AGACTGAACT AGCAAATAAA 
GAACAAGCTG ACTCCAGAAT ACATTCGAGT ATGGCAGATC CATACTCCAC CCTCAGTCGA 
GTGGGAAGTG TAAACTCCAC CCTGAAACGC TCAGACACCC GTGATTCCTG TTGTCAAACA 
GAAGAGGTGA AGATTGTTCC TCCCTCAGTA CGCAGAATCA GAGCACAAAG AGGCCATGGA 
ATAGCTGCCC AAATGGCCAG TGTCTCCTCC TCAAGCAGCA TTTCCACCAT AAGTGACTGT 
AATGGCATTA GCTACACACA GCTTAATGAC AGTGATCAGA GCTTTCAAAG TTTACCAAGA 
CAAGGTTCTC GCATCCCGCA ACAACACAGT GAAACAAAGT ACAATAGCTC CCCCTACAGG 
GCGAATTCTG GTTCTACAAG CCCCTTGTCC TACCATTTTA ACATGCAGCA TGACTCTTCA 
TCATCGCTGC ATATGCTGTC TGACTCGAGA AGCAAGCTTA CTGAAAATCT AAATTACGAA 
GGCTCTGATA AAAATAATAC CTCAAGCTTG TTTAGTCTCA GTGGTGCTCT ACAGAACTCC 
ATTCCTTATG GATACAAAAG GACAGAGGAC AACCAAATGC AATCACCTCA TTCGTCAGTC 
CAGTTGCTCA ATTCTGAGGA TGCCTCATTG TCGGGTGTCT CCTCGTGTTA TGAATCCACA 
GCGTCCCTCT GTACAGGTAT TCATACAGAG ACTGAGTCCC AGTGCAGCAC ACTTGATGGC 
AGAAACTGCT GCAGTCCTAA CTGTGTCCGC AGGGATTCCA ACTTCTCAGA GAGCAGCAAC 
CAAAGCTGCA GCACACTAAC CACGGACCAA TGGACGTATG AAGTTTCTCC AAAAGACAAT 
GTTACTTCAA GTTGCTCCTC ACCTGTCAGC CATATATACC ACGCAGAGGA ACATTCTCCC 
AGCAAGACAG ATACAAGCTC ATTGTTCTCT GTTGACAATG AAGGTTACTA CACCTCCATG 
CGCCTAGACT CTGGTTTGAA GTCACACAGT CACGGCTGCA TCAATAAAGC AGGAGAACAA 
AAGCACAGTT TGTATGAGTG CAAAGACCAC CACAGTCAAA GTGATACTGC AAGTCTGCAT 
AGCAACCGCT CCCTGACTCG TAGCATCTCT CTGCGAAAAG CCAGGAAGCC CCCACTCCCT 
CCAGAAAGAA CCGACTCTTT AAGGCGTAAA CCCCAGAGGA AGAGTCGCCA CAGTGGTTCA 
GTCCTTAATG AGCAGTTAAT CTCTAGTCTC CAGCAGTCCT TACAACTTAA CCTAAATACT 
CAAACTACAT CTTGCTCTGA ACAGTCACCC TGTGGTAGGT TTGAAGACCC TTGGGTCCTC 
CGACCGAGAA GTCAGAGTAT CATAAGTGCA GCTAGCAGTG GGGTATCTGC ACCAGCTGCT 
GTATGTCCGG TCACACCAAC ACACAGTGAC AGCAGCAGTC AGCGCTCAGA CTATGCAGAG 
TCATGGGACT TCTACATGGA TTGCCCATGT ACACGGTCTG ACCAGAGTCT TTCCTCACAA 
ATCACAAGGT CTGCAAGTGC TGAGGAGAAT CATGGAGTCA TAGGTAATGC ATCCTACAAC 
AGTGAATTCC CATCATCCCC CTTCAGCAGT AGTCAGTCAA AATTGACCTC ATCTCCTGAC 
AAAGTCCATC GACTCACGTC ACCATCAAGT GGCTACTCAA GTCAGTCTAA CACTCCAACT 
GCAGGCACTC CAGTTACGTC TCTAATAAGA GCAAAGTCTC CTGCGGGGAG ACCGAAACCA 
AAAGTGCCAG AAAGAAAGTC CTCTCTACGA TCTTCTATAT CATCCTCTTC TACATCCCTG 
TCCTCCAACA CCTCAGATTC CATCAGGAAC ATTCCTCCAC CTCCACCTTT ACCTGACATG 
ACAACTGCAT TATGTGGGTC GCCCAGTTCT GCTTCTTTCT CTCCAACAGC GGCTAAAATA 
GACCCAATAC CCTCTTTTAC ATCTCTACAG GATGTATCAT CTGCCTTGGA AGATGGGCAA 
AATACACTTT CAGATACATC TGCAACACCT GAGGAATGTG ATAGTCCTGA TTTTCCCCCT 
CCCCATTCAA ATGTTTTTGA ATCTCTGTCA GGCAATGTAG ATACCAACAG CCTCCCACCT 
CCTCCACTTC TGCTTTGCAA ACCTTCACTG TGCATTTCAT CTCTTCTTCC CCCTCCTCCT 
CCACCTCCTC CACTATTACT TGAGACCAGT CTTCAACCAG CCACCATTTT GAAGACAGAG 
AGCAGTCTTA CACCTACAAC AGCTGTAAAA TGTGAAAAAG AAGTAAACAT CAGTCACACT 
GGAGAGCAAG AAAAGACTCA GAGACCAATA ATCACTGCCC AGGCCCTTCA GATGGTGCAG 
TTGCGACCTG TTAAGCTCAA GCAAATAGAA ACTATTTTTA CATCTGTCAG TTTTGATCAT 
TCTGATCACC AAGCACAAAA CCATTCTGAT ACAAACTCAG GAAAGTCCCT GGAAATTGAT 
AATACAGGGC AAACAGTGAC CGTCAATTGT GTTACTCAGT CAGAAAATAG TATGGAAACA 
TCTGAATGTG AATATTCGGT GAACAGATTC ATAGACGATC TCCCTGAGGT ATCAACTAGC 
ACTCTACCCC CTGACCTAGA TGAACAATTA AAAAAAACTG AGCAAAATAA TCCAGTGCAC 
CTCTCTTCCA AGTCCACAAA TTCCTTAGCT TCTCCTCTGA GTTCTCCAAT GAAACAGAAG 
CCTCCTATAT CACCCAAAAA ACCCAATCTT TCCCTTATTA TACCACCCAT AATGCACTTG 
CCATTTTCCA ACGGACTCAA GCTTCAGCAG GTTTATGAAG CACAAGAGAC ACTAGAGTCA 
GAGCAAGTGA ATGGTGCATC CTCGACAAAC ACAGTTAATT TGCAAGCAAT TCCTTTAGAC 
CTTGAGGTAG AGGAAGAGAG TGACTCAGAC TTATCAACCC CTGTGGTCAA GAGCAGACTA 
TCTTTCTGCA GCGAGTTATC ATCAAGCAGT TTCCAAGACC TGCAACTTTC TGATTACATT 
CTTAATGAGC ATGACCTCGG CCTGAGCGAT AAAGACTTTG GGCTCTGTGA TGATAAAAGC 
ACATCAGATG ATGGTTCAAG CAGCAGCTCA GGATCCATCA GCTTTAAAGA GGATGAACAT 
GAGGAAAACG GTGCTGTGTT TGACTCAATC ACTGAAAGCA CATCTGACAT TAATGCTAGC 
GGAGAAGCTG TGGAGGGGAT GGTGACGCCT ATTCGCCCTC GTACTACAGA GGACCTCTTT 
GCTGCAATTC ATAGGTCAAA AAGGAAGGTC CTGGGCCGTG GCGATTCTGA AGATGAACGC 
TGCCGTAACC TCCTTCCTTC CCCACCTGTC ACTCCAACTG GCTCCTGCCC CAGCTTGCCA 
CGTCAGACCA GCTCCATCCA GCGGAACCTA CGTCGCTCCA CCACAAGCAA TGACAGTTTT 
AAAGCTTTGC TACTCAAGAA GGGCAGCCGC TCAGAGTCCA GCTTTCGCAT GTCAGCCGCT 
GAGATTCTCA AATGCACAGA CCCCCGTTTC CAAAGGGCTA ATTCAGAAAA TGCACTAGCT 
GACGGACTGT CCTCTCCGAC AAGCAGCAGG AGAGCACATG AAGAGTGGGC ACGGACGGAG 
GGAGCTTTGC CTCGCTTTAC CACTGGACAA ACTATCCCAA AATATGGGCG CTCGCGCACA 
CCACCTTCCG CAGCCAGCAG CCGGTATAAC AGCCGCAGCC GGATTACCAG TAGGCCAATG 
ACTGCCATCT GTGAACGAGA TGGAGAGGTG GCTGAATCTA CAGACTGCTA CTTAAGTAGC 
GAGAATCATT TTGTCTTGGC TTTATCCTCC AGCAGCATGG TCTGCGCTCA AGGCAGCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467439.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323114.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa22652
Clone ID Related Accession (Same CDS sequence)	XM_005158822.3 , XM_021467439.1
Accession Version	XM_021467439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGT GTGGGACGAA GTTGGGGGGG GCAGTTGCTC CGGCTGGGGC GCTGGGCTGT 
GTGAGGACAG TGCGCTGCAA AACTCTTTTG ACACACTTCC CAGCAAATAC CAACACACAG 
CGGGAGAGGC AGGAGACCAT GTTCTGTCTG AGAGCGGTGT CCAGTCTTGA TGAGGAGAGC 
AAATGGTCGG TGCACTACAC AGCCCCCTGG CACCACCAAG AGAACGTCTT CCTGCCTGGG 
TCCAGACCAG CATGTGTTGA GGATCTTCAC CGTCAGGCCA AAGTCAACCT GAAAACAGTC 
CTCAGAGAGT GTGACAAGCT CAGAAAAGAT GGCTTTCGCA GTTCTCAGTA TTACTCCCAA 
AGCCCAGCAT TCTCTGCAAC CAGCCTTTGT GAAAGTGTAC ATCTGGATGA AGAGAAGACA 
AAGAAAAAGA AGCGGTTTAA AGTCCTAGAC TGCCTATCAC AGTCTTGCCT CTCTCTCTGC 
TGTCATTTGA GATCTTGGAA AACAAAGGCT TCCGATTCAC CTAATGAAGA GGATAAACTT 
GTGTACTCAA TGCCGCCACA AACCCCTCTG CTGGAGCATG GCTCTGACAT GGACATCCAG 
AGAAGCTGGA CCAGCTGTCT ACCTTTGCCC ACTCCCGAGG AGAAGATGAG ACTGCAGGCC 
CAATCGGTGG CTACTGATAT AATTCCTATC AACATTACAG GTGAGAACTT TGACCGCCAG 
GCCAGCTTTC GCCGCTCCAT GGCAAACACT GATACTATTA TACGAAGACC TAAGAGAGTT 
CAAAGAAGAA AGACAATAAC AGGAGTGCCT GACAACATCC AGACTGAACT AGCAAATAAA 
GAACAAGCTG ACTCCAGAAT ACATTCGAGT ATGGCAGATC CATACTCCAC CCTCAGTCGA 
GTGGGAAGTG TAAACTCCAC CCTGAAACGC TCAGACACCC GTGATTCCTG TTGTCAAACA 
GAAGAGGTGA AGATTGTTCC TCCCTCAGTA CGCAGAATCA GAGCACAAAG AGGCCATGGA 
ATAGCTGCCC AAATGGCCAG TGTCTCCTCC TCAAGCAGCA TTTCCACCAT AAGTGACTGT 
AATGGCATTA GCTACACACA GCTTAATGAC AGTGATCAGA GCTTTCAAAG TTTACCAAGA 
CAAGGTTCTC GCATCCCGCA ACAACACAGT GAAACAAAGT ACAATAGCTC CCCCTACAGG 
GCGAATTCTG GTTCTACAAG CCCCTTGTCC TACCATTTTA ACATGCAGCA TGACTCTTCA 
TCATCGCTGC ATATGCTGTC TGACTCGAGA AGCAAGCTTA CTGAAAATCT AAATTACGAA 
GGCTCTGATA AAAATAATAC CTCAAGCTTG TTTAGTCTCA GTGGTGCTCT ACAGAACTCC 
ATTCCTTATG GATACAAAAG GACAGAGGAC AACCAAATGC AATCACCTCA TTCGTCAGTC 
CAGTTGCTCA ATTCTGAGGA TGCCTCATTG TCGGGTGTCT CCTCGTGTTA TGAATCCACA 
GCGTCCCTCT GTACAGGTAT TCATACAGAG ACTGAGTCCC AGTGCAGCAC ACTTGATGGC 
AGAAACTGCT GCAGTCCTAA CTGTGTCCGC AGGGATTCCA ACTTCTCAGA GAGCAGCAAC 
CAAAGCTGCA GCACACTAAC CACGGACCAA TGGACGTATG AAGTTTCTCC AAAAGACAAT 
GTTACTTCAA GTTGCTCCTC ACCTGTCAGC CATATATACC ACGCAGAGGA ACATTCTCCC 
AGCAAGACAG ATACAAGCTC ATTGTTCTCT GTTGACAATG AAGGTTACTA CACCTCCATG 
CGCCTAGACT CTGGTTTGAA GTCACACAGT CACGGCTGCA TCAATAAAGC AGGAGAACAA 
AAGCACAGTT TGTATGAGTG CAAAGACCAC CACAGTCAAA GTGATACTGC AAGTCTGCAT 
AGCAACCGCT CCCTGACTCG TAGCATCTCT CTGCGAAAAG CCAGGAAGCC CCCACTCCCT 
CCAGAAAGAA CCGACTCTTT AAGGCGTAAA CCCCAGAGGA AGAGTCGCCA CAGTGGTTCA 
GTCCTTAATG AGCAGTTAAT CTCTAGTCTC CAGCAGTCCT TACAACTTAA CCTAAATACT 
CAAACTACAT CTTGCTCTGA ACAGTCACCC TGTGGTAGGT TTGAAGACCC TTGGGTCCTC 
CGACCGAGAA GTCAGAGTAT CATAAGTGCA GCTAGCAGTG GGGTATCTGC ACCAGCTGCT 
GTATGTCCGG TCACACCAAC ACACAGTGAC AGCAGCAGTC AGCGCTCAGA CTATGCAGAG 
TCATGGGACT TCTACATGGA TTGCCCATGT ACACGGTCTG ACCAGAGTCT TTCCTCACAA 
ATCACAAGGT CTGCAAGTGC TGAGGAGAAT CATGGAGTCA TAGGTAATGC ATCCTACAAC 
AGTGAATTCC CATCATCCCC CTTCAGCAGT AGTCAGTCAA AATTGACCTC ATCTCCTGAC 
AAAGTCCATC GACTCACGTC ACCATCAAGT GGCTACTCAA GTCAGTCTAA CACTCCAACT 
GCAGGCACTC CAGTTACGTC TCTAATAAGA GCAAAGTCTC CTGCGGGGAG ACCGAAACCA 
AAAGTGCCAG AAAGAAAGTC CTCTCTACGA TCTTCTATAT CATCCTCTTC TACATCCCTG 
TCCTCCAACA CCTCAGATTC CATCAGGAAC ATTCCTCCAC CTCCACCTTT ACCTGACATG 
ACAACTGCAT TATGTGGGTC GCCCAGTTCT GCTTCTTTCT CTCCAACAGC GGCTAAAATA 
GACCCAATAC CCTCTTTTAC ATCTCTACAG GATGTATCAT CTGCCTTGGA AGATGGGCAA 
AATACACTTT CAGATACATC TGCAACACCT GAGGAATGTG ATAGTCCTGA TTTTCCCCCT 
CCCCATTCAA ATGTTTTTGA ATCTCTGTCA GGCAATGTAG ATACCAACAG CCTCCCACCT 
CCTCCACTTC TGCTTTGCAA ACCTTCACTG TGCATTTCAT CTCTTCTTCC CCCTCCTCCT 
CCACCTCCTC CACTATTACT TGAGACCAGT CTTCAACCAG CCACCATTTT GAAGACAGAG 
AGCAGTCTTA CACCTACAAC AGCTGTAAAA TGTGAAAAAG AAGTAAACAT CAGTCACACT 
GGAGAGCAAG AAAAGACTCA GAGACCAATA ATCACTGCCC AGGCCCTTCA GATGGTGCAG 
TTGCGACCTG TTAAGCTCAA GCAAATAGAA ACTATTTTTA CATCTGTCAG TTTTGATCAT 
TCTGATCACC AAGCACAAAA CCATTCTGAT ACAAACTCAG GAAAGTCCCT GGAAATTGAT 
AATACAGGGC AAACAGTGAC CGTCAATTGT GTTACTCAGT CAGAAAATAG TATGGAAACA 
TCTGAATGTG AATATTCGGT GAACAGATTC ATAGACGATC TCCCTGAGGT ATCAACTAGC 
ACTCTACCCC CTGACCTAGA TGAACAATTA AAAAAAACTG AGCAAAATAA TCCAGTGCAC 
CTCTCTTCCA AGTCCACAAA TTCCTTAGCT TCTCCTCTGA GTTCTCCAAT GAAACAGAAG 
CCTCCTATAT CACCCAAAAA ACCCAATCTT TCCCTTATTA TACCACCCAT AATGCACTTG 
CCATTTTCCA ACGGACTCAA GCTTCAGCAG GTTTATGAAG CACAAGAGAC ACTAGAGTCA 
GAGCAAGTGA ATGGTGCATC CTCGACAAAC ACAGTTAATT TGCAAGCAAT TCCTTTAGAC 
CTTGAGGTAG AGGAAGAGAG TGACTCAGAC TTATCAACCC CTGTGGTCAA GAGCAGACTA 
TCTTTCTGCA GCGAGTTATC ATCAAGCAGT TTCCAAGACC TGCAACTTTC TGATTACATT 
CTTAATGAGC ATGACCTCGG CCTGAGCGAT AAAGACTTTG GGCTCTGTGA TGATAAAAGC 
ACATCAGATG ATGGTTCAAG CAGCAGCTCA GGATCCATCA GCTTTAAAGA GGATGAACAT 
GAGGAAAACG GTGCTGTGTT TGACTCAATC ACTGAAAGCA CATCTGACAT TAATGCTAGC 
GGAGAAGCTG TGGAGGGGAT GGTGACGCCT ATTCGCCCTC GTACTACAGA GGACCTCTTT 
GCTGCAATTC ATAGGTCAAA AAGGAAGGTC CTGGGCCGTG GCGATTCTGA AGATGAACGC 
TGCCGTAACC TCCTTCCTTC CCCACCTGTC ACTCCAACTG GCTCCTGCCC CAGCTTGCCA 
CGTCAGACCA GCTCCATCCA GCGGAACCTA CGTCGCTCCA CCACAAGCAA TGACAGTTTT 
AAAGCTTTGC TACTCAAGAA GGGCAGCCGC TCAGAGTCCA GCTTTCGCAT GTCAGCCGCT 
GAGATTCTCA AATGCACAGA CCCCCGTTTC CAAAGGGCTA ATTCAGAAAA TGCACTAGCT 
GACGGACTGT CCTCTCCGAC AAGCAGCAGG AGAGCACATG AAGAGTGGGC ACGGACGGAG 
GGAGCTTTGC CTCGCTTTAC CACTGGACAA ACTATCCCAA AATATGGGCG CTCGCGCACA 
CCACCTTCCG CAGCCAGCAG CCGGTATAAC AGCCGCAGCC GGATTACCAG TAGGCCAATG 
ACTGCCATCT GTGAACGAGA TGGAGAGGTG GCTGAATCTA CAGACTGCTA CTTAAGTAGC 
GAGAATCATT TTGTCTTGGC TTTATCCTCC AGCAGCATGG TCTGCGCTCA AGGCAGCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323114.1
CDS	168..4850
Translation	MNLCGTKLGGAVAPAGALGCVRTVRCKTLLTHFPANTNTQRERQETMFCLRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467439.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467439.1

ORF Insert Sequence:

ATGAACCTGT GTGGGACGAA GTTGGGGGGG GCAGTTGCTC CGGCTGGGGC GCTGGGCTGT 
GTGAGGACAG TGCGCTGCAA AACTCTTTTG ACACACTTCC CAGCAAATAC CAACACACAG 
CGGGAGAGGC AGGAGACCAT GTTCTGTCTG AGAGCGGTGT CCAGTCTTGA TGAGGAGAGC 
AAATGGTCGG TGCACTACAC AGCCCCCTGG CACCACCAAG AGAACGTCTT CCTGCCTGGG 
TCCAGACCAG CATGTGTTGA GGATCTTCAC CGTCAGGCCA AAGTCAACCT GAAAACAGTC 
CTCAGAGAGT GTGACAAGCT CAGAAAAGAT GGCTTTCGCA GTTCTCAGTA TTACTCCCAA 
AGCCCAGCAT TCTCTGCAAC CAGCCTTTGT GAAAGTGTAC ATCTGGATGA AGAGAAGACA 
AAGAAAAAGA AGCGGTTTAA AGTCCTAGAC TGCCTATCAC AGTCTTGCCT CTCTCTCTGC 
TGTCATTTGA GATCTTGGAA AACAAAGGCT TCCGATTCAC CTAATGAAGA GGATAAACTT 
GTGTACTCAA TGCCGCCACA AACCCCTCTG CTGGAGCATG GCTCTGACAT GGACATCCAG 
AGAAGCTGGA CCAGCTGTCT ACCTTTGCCC ACTCCCGAGG AGAAGATGAG ACTGCAGGCC 
CAATCGGTGG CTACTGATAT AATTCCTATC AACATTACAG GTGAGAACTT TGACCGCCAG 
GCCAGCTTTC GCCGCTCCAT GGCAAACACT GATACTATTA TACGAAGACC TAAGAGAGTT 
CAAAGAAGAA AGACAATAAC AGGAGTGCCT GACAACATCC AGACTGAACT AGCAAATAAA 
GAACAAGCTG ACTCCAGAAT ACATTCGAGT ATGGCAGATC CATACTCCAC CCTCAGTCGA 
GTGGGAAGTG TAAACTCCAC CCTGAAACGC TCAGACACCC GTGATTCCTG TTGTCAAACA 
GAAGAGGTGA AGATTGTTCC TCCCTCAGTA CGCAGAATCA GAGCACAAAG AGGCCATGGA 
ATAGCTGCCC AAATGGCCAG TGTCTCCTCC TCAAGCAGCA TTTCCACCAT AAGTGACTGT 
AATGGCATTA GCTACACACA GCTTAATGAC AGTGATCAGA GCTTTCAAAG TTTACCAAGA 
CAAGGTTCTC GCATCCCGCA ACAACACAGT GAAACAAAGT ACAATAGCTC CCCCTACAGG 
GCGAATTCTG GTTCTACAAG CCCCTTGTCC TACCATTTTA ACATGCAGCA TGACTCTTCA 
TCATCGCTGC ATATGCTGTC TGACTCGAGA AGCAAGCTTA CTGAAAATCT AAATTACGAA 
GGCTCTGATA AAAATAATAC CTCAAGCTTG TTTAGTCTCA GTGGTGCTCT ACAGAACTCC 
ATTCCTTATG GATACAAAAG GACAGAGGAC AACCAAATGC AATCACCTCA TTCGTCAGTC 
CAGTTGCTCA ATTCTGAGGA TGCCTCATTG TCGGGTGTCT CCTCGTGTTA TGAATCCACA 
GCGTCCCTCT GTACAGGTAT TCATACAGAG ACTGAGTCCC AGTGCAGCAC ACTTGATGGC 
AGAAACTGCT GCAGTCCTAA CTGTGTCCGC AGGGATTCCA ACTTCTCAGA GAGCAGCAAC 
CAAAGCTGCA GCACACTAAC CACGGACCAA TGGACGTATG AAGTTTCTCC AAAAGACAAT 
GTTACTTCAA GTTGCTCCTC ACCTGTCAGC CATATATACC ACGCAGAGGA ACATTCTCCC 
AGCAAGACAG ATACAAGCTC ATTGTTCTCT GTTGACAATG AAGGTTACTA CACCTCCATG 
CGCCTAGACT CTGGTTTGAA GTCACACAGT CACGGCTGCA TCAATAAAGC AGGAGAACAA 
AAGCACAGTT TGTATGAGTG CAAAGACCAC CACAGTCAAA GTGATACTGC AAGTCTGCAT 
AGCAACCGCT CCCTGACTCG TAGCATCTCT CTGCGAAAAG CCAGGAAGCC CCCACTCCCT 
CCAGAAAGAA CCGACTCTTT AAGGCGTAAA CCCCAGAGGA AGAGTCGCCA CAGTGGTTCA 
GTCCTTAATG AGCAGTTAAT CTCTAGTCTC CAGCAGTCCT TACAACTTAA CCTAAATACT 
CAAACTACAT CTTGCTCTGA ACAGTCACCC TGTGGTAGGT TTGAAGACCC TTGGGTCCTC 
CGACCGAGAA GTCAGAGTAT CATAAGTGCA GCTAGCAGTG GGGTATCTGC ACCAGCTGCT 
GTATGTCCGG TCACACCAAC ACACAGTGAC AGCAGCAGTC AGCGCTCAGA CTATGCAGAG 
TCATGGGACT TCTACATGGA TTGCCCATGT ACACGGTCTG ACCAGAGTCT TTCCTCACAA 
ATCACAAGGT CTGCAAGTGC TGAGGAGAAT CATGGAGTCA TAGGTAATGC ATCCTACAAC 
AGTGAATTCC CATCATCCCC CTTCAGCAGT AGTCAGTCAA AATTGACCTC ATCTCCTGAC 
AAAGTCCATC GACTCACGTC ACCATCAAGT GGCTACTCAA GTCAGTCTAA CACTCCAACT 
GCAGGCACTC CAGTTACGTC TCTAATAAGA GCAAAGTCTC CTGCGGGGAG ACCGAAACCA 
AAAGTGCCAG AAAGAAAGTC CTCTCTACGA TCTTCTATAT CATCCTCTTC TACATCCCTG 
TCCTCCAACA CCTCAGATTC CATCAGGAAC ATTCCTCCAC CTCCACCTTT ACCTGACATG 
ACAACTGCAT TATGTGGGTC GCCCAGTTCT GCTTCTTTCT CTCCAACAGC GGCTAAAATA 
GACCCAATAC CCTCTTTTAC ATCTCTACAG GATGTATCAT CTGCCTTGGA AGATGGGCAA 
AATACACTTT CAGATACATC TGCAACACCT GAGGAATGTG ATAGTCCTGA TTTTCCCCCT 
CCCCATTCAA ATGTTTTTGA ATCTCTGTCA GGCAATGTAG ATACCAACAG CCTCCCACCT 
CCTCCACTTC TGCTTTGCAA ACCTTCACTG TGCATTTCAT CTCTTCTTCC CCCTCCTCCT 
CCACCTCCTC CACTATTACT TGAGACCAGT CTTCAACCAG CCACCATTTT GAAGACAGAG 
AGCAGTCTTA CACCTACAAC AGCTGTAAAA TGTGAAAAAG AAGTAAACAT CAGTCACACT 
GGAGAGCAAG AAAAGACTCA GAGACCAATA ATCACTGCCC AGGCCCTTCA GATGGTGCAG 
TTGCGACCTG TTAAGCTCAA GCAAATAGAA ACTATTTTTA CATCTGTCAG TTTTGATCAT 
TCTGATCACC AAGCACAAAA CCATTCTGAT ACAAACTCAG GAAAGTCCCT GGAAATTGAT 
AATACAGGGC AAACAGTGAC CGTCAATTGT GTTACTCAGT CAGAAAATAG TATGGAAACA 
TCTGAATGTG AATATTCGGT GAACAGATTC ATAGACGATC TCCCTGAGGT ATCAACTAGC 
ACTCTACCCC CTGACCTAGA TGAACAATTA AAAAAAACTG AGCAAAATAA TCCAGTGCAC 
CTCTCTTCCA AGTCCACAAA TTCCTTAGCT TCTCCTCTGA GTTCTCCAAT GAAACAGAAG 
CCTCCTATAT CACCCAAAAA ACCCAATCTT TCCCTTATTA TACCACCCAT AATGCACTTG 
CCATTTTCCA ACGGACTCAA GCTTCAGCAG GTTTATGAAG CACAAGAGAC ACTAGAGTCA 
GAGCAAGTGA ATGGTGCATC CTCGACAAAC ACAGTTAATT TGCAAGCAAT TCCTTTAGAC 
CTTGAGGTAG AGGAAGAGAG TGACTCAGAC TTATCAACCC CTGTGGTCAA GAGCAGACTA 
TCTTTCTGCA GCGAGTTATC ATCAAGCAGT TTCCAAGACC TGCAACTTTC TGATTACATT 
CTTAATGAGC ATGACCTCGG CCTGAGCGAT AAAGACTTTG GGCTCTGTGA TGATAAAAGC 
ACATCAGATG ATGGTTCAAG CAGCAGCTCA GGATCCATCA GCTTTAAAGA GGATGAACAT 
GAGGAAAACG GTGCTGTGTT TGACTCAATC ACTGAAAGCA CATCTGACAT TAATGCTAGC 
GGAGAAGCTG TGGAGGGGAT GGTGACGCCT ATTCGCCCTC GTACTACAGA GGACCTCTTT 
GCTGCAATTC ATAGGTCAAA AAGGAAGGTC CTGGGCCGTG GCGATTCTGA AGATGAACGC 
TGCCGTAACC TCCTTCCTTC CCCACCTGTC ACTCCAACTG GCTCCTGCCC CAGCTTGCCA 
CGTCAGACCA GCTCCATCCA GCGGAACCTA CGTCGCTCCA CCACAAGCAA TGACAGTTTT 
AAAGCTTTGC TACTCAAGAA GGGCAGCCGC TCAGAGTCCA GCTTTCGCAT GTCAGCCGCT 
GAGATTCTCA AATGCACAGA CCCCCGTTTC CAAAGGGCTA ATTCAGAAAA TGCACTAGCT 
GACGGACTGT CCTCTCCGAC AAGCAGCAGG AGAGCACATG AAGAGTGGGC ACGGACGGAG 
GGAGCTTTGC CTCGCTTTAC CACTGGACAA ACTATCCCAA AATATGGGCG CTCGCGCACA 
CCACCTTCCG CAGCCAGCAG CCGGTATAAC AGCCGCAGCC GGATTACCAG TAGGCCAATG 
ACTGCCATCT GTGAACGAGA TGGAGAGGTG GCTGAATCTA CAGACTGCTA CTTAAGTAGC 
GAGAATCATT TTGTCTTGGC TTTATCCTCC AGCAGCATGG TCTGCGCTCA AGGCAGCACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158823.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158880.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa22653
Clone ID Related Accession (Same CDS sequence)	XM_005158823.3 , XM_021467440.1
Accession Version	XM_005158823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGCGGTTTA AAGTCCTAGA CTGCCTATCA 
CAGTCTTGCC TCTCTCTCTG CTGTCATTTG AGATCTTGGA AAACAAAGGC TTCCGATTCA 
CCTAATGAAG AGGATAAACT TGTGTACTCA ATGCCGCCAC AAACCCCTCT GCTGGAGCAT 
GGCTCTGACA TGGACATCCA GAGAAGCTGG ACCAGCTGTC TACCTTTGCC CACTCCCGAG 
GAGAAGATGA GACTGCAGGC CCAATCGGTG GCTACTGATA TAATTCCTAT CAACATTACA 
GGTGAGAACT TTGACCGCCA GGCCAGCTTT CGCCGCTCCA TGGCAAACAC TGATACTATT 
ATACGAAGAC CTAAGAGAGT TCAAAGAAGA AAGACAATAA CAGGAGTGCC TGACAACATC 
CAGACTGAAC TAGCAAATAA AGAACAAGCT GACTCCAGAA TACATTCGAG TATGGCAGAT 
CCATACTCCA CCCTCAGTCG AGTGGGAAGT GTAAACTCCA CCCTGAAACG CTCAGACACC 
CGTGATTCCT GTTGTCAAAC AGAAGAGGTG AAGATTGTTC CTCCCTCAGT ACGCAGAATC 
AGAGCACAAA GAGGCCATGG AATAGCTGCC CAAATGGCCA GTGTCTCCTC CTCAAGCAGC 
ATTTCCACCA TAAGTGACTG TAATGGCATT AGCTACACAC AGCTTAATGA CAGTGATCAG 
AGCTTTCAAA GTTTACCAAG ACAAGGTTCT CGCATCCCGC AACAACACAG TGAAACAAAG 
TACAATAGCT CCCCCTACAG GGCGAATTCT GGTTCTACAA GCCCCTTGTC CTACCATTTT 
AACATGCAGC ATGACTCTTC ATCATCGCTG CATATGCTGT CTGACTCGAG AAGCAAGCTT 
ACTGAAAATC TAAATTACGA AGGCTCTGAT AAAAATAATA CCTCAAGCTT GTTTAGTCTC 
AGTGGTGCTC TACAGAACTC CATTCCTTAT GGATACAAAA GGACAGAGGA CAACCAAATG 
CAATCACCTC ATTCGTCAGT CCAGTTGCTC AATTCTGAGG ATGCCTCATT GTCGGGTGTC 
TCCTCGTGTT ATGAATCCAC AGCGTCCCTC TGTACAGGTA TTCATACAGA GACTGAGTCC 
CAGTGCAGCA CACTTGATGG CAGAAACTGC TGCAGTCCTA ACTGTGTCCG CAGGGATTCC 
AACTTCTCAG AGAGCAGCAA CCAAAGCTGC AGCACACTAA CCACGGACCA ATGGACGTAT 
GAAGTTTCTC CAAAAGACAA TGTTACTTCA AGTTGCTCCT CACCTGTCAG CCATATATAC 
CACGCAGAGG AACATTCTCC CAGCAAGACA GATACAAGCT CATTGTTCTC TGTTGACAAT 
GAAGGTTACT ACACCTCCAT GCGCCTAGAC TCTGGTTTGA AGTCACACAG TCACGGCTGC 
ATCAATAAAG CAGGAGAACA AAAGCACAGT TTGTATGAGT GCAAAGACCA CCACAGTCAA 
AGTGATACTG CAAGTCTGCA TAGCAACCGC TCCCTGACTC GTAGCATCTC TCTGCGAAAA 
GCCAGGAAGC CCCCACTCCC TCCAGAAAGA ACCGACTCTT TAAGGCGTAA ACCCCAGAGG 
AAGAGTCGCC ACAGTGGTTC AGTCCTTAAT GAGCAGTTAA TCTCTAGTCT CCAGCAGTCC 
TTACAACTTA ACCTAAATAC TCAAACTACA TCTTGCTCTG AACAGTCACC CTGTGGTAGG 
TTTGAAGACC CTTGGGTCCT CCGACCGAGA AGTCAGAGTA TCATAAGTGC AGCTAGCAGT 
GGGGTATCTG CACCAGCTGC TGTATGTCCG GTCACACCAA CACACAGTGA CAGCAGCAGT 
CAGCGCTCAG ACTATGCAGA GTCATGGGAC TTCTACATGG ATTGCCCATG TACACGGTCT 
GACCAGAGTC TTTCCTCACA AATCACAAGG TCTGCAAGTG CTGAGGAGAA TCATGGAGTC 
ATAGGTAATG CATCCTACAA CAGTGAATTC CCATCATCCC CCTTCAGCAG TAGTCAGTCA 
AAATTGACCT CATCTCCTGA CAAAGTCCAT CGACTCACGT CACCATCAAG TGGCTACTCA 
AGTCAGTCTA ACACTCCAAC TGCAGGCACT CCAGTTACGT CTCTAATAAG AGCAAAGTCT 
CCTGCGGGGA GACCGAAACC AAAAGTGCCA GAAAGAAAGT CCTCTCTACG ATCTTCTATA 
TCATCCTCTT CTACATCCCT GTCCTCCAAC ACCTCAGATT CCATCAGGAA CATTCCTCCA 
CCTCCACCTT TACCTGACAT GACAACTGCA TTATGTGGGT CGCCCAGTTC TGCTTCTTTC 
TCTCCAACAG CGGCTAAAAT AGACCCAATA CCCTCTTTTA CATCTCTACA GGATGTATCA 
TCTGCCTTGG AAGATGGGCA AAATACACTT TCAGATACAT CTGCAACACC TGAGGAATGT 
GATAGTCCTG ATTTTCCCCC TCCCCATTCA AATGTTTTTG AATCTCTGTC AGGCAATGTA 
GATACCAACA GCCTCCCACC TCCTCCACTT CTGCTTTGCA AACCTTCACT GTGCATTTCA 
TCTCTTCTTC CCCCTCCTCC TCCACCTCCT CCACTATTAC TTGAGACCAG TCTTCAACCA 
GCCACCATTT TGAAGACAGA GAGCAGTCTT ACACCTACAA CAGCTGTAAA ATGTGAAAAA 
GAAGTAAACA TCAGTCACAC TGGAGAGCAA GAAAAGACTC AGAGACCAAT AATCACTGCC 
CAGGCCCTTC AGATGGTGCA GTTGCGACCT GTTAAGCTCA AGCAAATAGA AACTATTTTT 
ACATCTGTCA GTTTTGATCA TTCTGATCAC CAAGCACAAA ACCATTCTGA TACAAACTCA 
GGAAAGTCCC TGGAAATTGA TAATACAGGG CAAACAGTGA CCGTCAATTG TGTTACTCAG 
TCAGAAAATA GTATGGAAAC ATCTGAATGT GAATATTCGG TGAACAGATT CATAGACGAT 
CTCCCTGAGG TATCAACTAG CACTCTACCC CCTGACCTAG ATGAACAATT AAAAAAAACT 
GAGCAAAATA ATCCAGTGCA CCTCTCTTCC AAGTCCACAA ATTCCTTAGC TTCTCCTCTG 
AGTTCTCCAA TGAAACAGAA GCCTCCTATA TCACCCAAAA AACCCAATCT TTCCCTTATT 
ATACCACCCA TAATGCACTT GCCATTTTCC AACGGACTCA AGCTTCAGCA GGTTTATGAA 
GCACAAGAGA CACTAGAGTC AGAGCAAGTG AATGGTGCAT CCTCGACAAA CACAGTTAAT 
TTGCAAGCAA TTCCTTTAGA CCTTGAGGTA GAGGAAGAGA GTGACTCAGA CTTATCAACC 
CCTGTGGTCA AGAGCAGACT ATCTTTCTGC AGCGAGTTAT CATCAAGCAG TTTCCAAGAC 
CTGCAACTTT CTGATTACAT TCTTAATGAG CATGACCTCG GCCTGAGCGA TAAAGACTTT 
GGGCTCTGTG ATGATAAAAG CACATCAGAT GATGGTTCAA GCAGCAGCTC AGGATCCATC 
AGCTTTAAAG AGGATGAACA TGAGGAAAAC GGTGCTGTGT TTGACTCAAT CACTGAAAGC 
ACATCTGACA TTAATGCTAG CGGAGAAGCT GTGGAGGGGA TGGTGACGCC TATTCGCCCT 
CGTACTACAG AGGACCTCTT TGCTGCAATT CATAGGTCAA AAAGGAAGGT CCTGGGCCGT 
GGCGATTCTG AAGATGAACG CTGCCGTAAC CTCCTTCCTT CCCCACCTGT CACTCCAACT 
GGCTCCTGCC CCAGCTTGCC ACGTCAGACC AGCTCCATCC AGCGGAACCT ACGTCGCTCC 
ACCACAAGCA ATGACAGTTT TAAAGCTTTG CTACTCAAGA AGGGCAGCCG CTCAGAGTCC 
AGCTTTCGCA TGTCAGCCGC TGAGATTCTC AAATGCACAG ACCCCCGTTT CCAAAGGGCT 
AATTCAGAAA ATGCACTAGC TGACGGACTG TCCTCTCCGA CAAGCAGCAG GAGAGCACAT 
GAAGAGTGGG CACGGACGGA GGGAGCTTTG CCTCGCTTTA CCACTGGACA AACTATCCCA 
AAATATGGGC GCTCGCGCAC ACCACCTTCC GCAGCCAGCA GCCGGTATAA CAGCCGCAGC 
CGGATTACCA GTAGGCCAAT GACTGCCATC TGTGAACGAG ATGGAGAGGT GGCTGAATCT 
ACAGACTGCT ACTTAAGTAG CGAGAATCAT TTTGTCTTGG CTTTATCCTC CAGCAGCATG 
GTCTGCGCTC AAGGCAGCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158880.1
CDS	170..4753
Translation	MVFIGTTLKSVIKYFKKKAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_005158823.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158823.3

ORF Insert Sequence:

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGCGGTTTA AAGTCCTAGA CTGCCTATCA 
CAGTCTTGCC TCTCTCTCTG CTGTCATTTG AGATCTTGGA AAACAAAGGC TTCCGATTCA 
CCTAATGAAG AGGATAAACT TGTGTACTCA ATGCCGCCAC AAACCCCTCT GCTGGAGCAT 
GGCTCTGACA TGGACATCCA GAGAAGCTGG ACCAGCTGTC TACCTTTGCC CACTCCCGAG 
GAGAAGATGA GACTGCAGGC CCAATCGGTG GCTACTGATA TAATTCCTAT CAACATTACA 
GGTGAGAACT TTGACCGCCA GGCCAGCTTT CGCCGCTCCA TGGCAAACAC TGATACTATT 
ATACGAAGAC CTAAGAGAGT TCAAAGAAGA AAGACAATAA CAGGAGTGCC TGACAACATC 
CAGACTGAAC TAGCAAATAA AGAACAAGCT GACTCCAGAA TACATTCGAG TATGGCAGAT 
CCATACTCCA CCCTCAGTCG AGTGGGAAGT GTAAACTCCA CCCTGAAACG CTCAGACACC 
CGTGATTCCT GTTGTCAAAC AGAAGAGGTG AAGATTGTTC CTCCCTCAGT ACGCAGAATC 
AGAGCACAAA GAGGCCATGG AATAGCTGCC CAAATGGCCA GTGTCTCCTC CTCAAGCAGC 
ATTTCCACCA TAAGTGACTG TAATGGCATT AGCTACACAC AGCTTAATGA CAGTGATCAG 
AGCTTTCAAA GTTTACCAAG ACAAGGTTCT CGCATCCCGC AACAACACAG TGAAACAAAG 
TACAATAGCT CCCCCTACAG GGCGAATTCT GGTTCTACAA GCCCCTTGTC CTACCATTTT 
AACATGCAGC ATGACTCTTC ATCATCGCTG CATATGCTGT CTGACTCGAG AAGCAAGCTT 
ACTGAAAATC TAAATTACGA AGGCTCTGAT AAAAATAATA CCTCAAGCTT GTTTAGTCTC 
AGTGGTGCTC TACAGAACTC CATTCCTTAT GGATACAAAA GGACAGAGGA CAACCAAATG 
CAATCACCTC ATTCGTCAGT CCAGTTGCTC AATTCTGAGG ATGCCTCATT GTCGGGTGTC 
TCCTCGTGTT ATGAATCCAC AGCGTCCCTC TGTACAGGTA TTCATACAGA GACTGAGTCC 
CAGTGCAGCA CACTTGATGG CAGAAACTGC TGCAGTCCTA ACTGTGTCCG CAGGGATTCC 
AACTTCTCAG AGAGCAGCAA CCAAAGCTGC AGCACACTAA CCACGGACCA ATGGACGTAT 
GAAGTTTCTC CAAAAGACAA TGTTACTTCA AGTTGCTCCT CACCTGTCAG CCATATATAC 
CACGCAGAGG AACATTCTCC CAGCAAGACA GATACAAGCT CATTGTTCTC TGTTGACAAT 
GAAGGTTACT ACACCTCCAT GCGCCTAGAC TCTGGTTTGA AGTCACACAG TCACGGCTGC 
ATCAATAAAG CAGGAGAACA AAAGCACAGT TTGTATGAGT GCAAAGACCA CCACAGTCAA 
AGTGATACTG CAAGTCTGCA TAGCAACCGC TCCCTGACTC GTAGCATCTC TCTGCGAAAA 
GCCAGGAAGC CCCCACTCCC TCCAGAAAGA ACCGACTCTT TAAGGCGTAA ACCCCAGAGG 
AAGAGTCGCC ACAGTGGTTC AGTCCTTAAT GAGCAGTTAA TCTCTAGTCT CCAGCAGTCC 
TTACAACTTA ACCTAAATAC TCAAACTACA TCTTGCTCTG AACAGTCACC CTGTGGTAGG 
TTTGAAGACC CTTGGGTCCT CCGACCGAGA AGTCAGAGTA TCATAAGTGC AGCTAGCAGT 
GGGGTATCTG CACCAGCTGC TGTATGTCCG GTCACACCAA CACACAGTGA CAGCAGCAGT 
CAGCGCTCAG ACTATGCAGA GTCATGGGAC TTCTACATGG ATTGCCCATG TACACGGTCT 
GACCAGAGTC TTTCCTCACA AATCACAAGG TCTGCAAGTG CTGAGGAGAA TCATGGAGTC 
ATAGGTAATG CATCCTACAA CAGTGAATTC CCATCATCCC CCTTCAGCAG TAGTCAGTCA 
AAATTGACCT CATCTCCTGA CAAAGTCCAT CGACTCACGT CACCATCAAG TGGCTACTCA 
AGTCAGTCTA ACACTCCAAC TGCAGGCACT CCAGTTACGT CTCTAATAAG AGCAAAGTCT 
CCTGCGGGGA GACCGAAACC AAAAGTGCCA GAAAGAAAGT CCTCTCTACG ATCTTCTATA 
TCATCCTCTT CTACATCCCT GTCCTCCAAC ACCTCAGATT CCATCAGGAA CATTCCTCCA 
CCTCCACCTT TACCTGACAT GACAACTGCA TTATGTGGGT CGCCCAGTTC TGCTTCTTTC 
TCTCCAACAG CGGCTAAAAT AGACCCAATA CCCTCTTTTA CATCTCTACA GGATGTATCA 
TCTGCCTTGG AAGATGGGCA AAATACACTT TCAGATACAT CTGCAACACC TGAGGAATGT 
GATAGTCCTG ATTTTCCCCC TCCCCATTCA AATGTTTTTG AATCTCTGTC AGGCAATGTA 
GATACCAACA GCCTCCCACC TCCTCCACTT CTGCTTTGCA AACCTTCACT GTGCATTTCA 
TCTCTTCTTC CCCCTCCTCC TCCACCTCCT CCACTATTAC TTGAGACCAG TCTTCAACCA 
GCCACCATTT TGAAGACAGA GAGCAGTCTT ACACCTACAA CAGCTGTAAA ATGTGAAAAA 
GAAGTAAACA TCAGTCACAC TGGAGAGCAA GAAAAGACTC AGAGACCAAT AATCACTGCC 
CAGGCCCTTC AGATGGTGCA GTTGCGACCT GTTAAGCTCA AGCAAATAGA AACTATTTTT 
ACATCTGTCA GTTTTGATCA TTCTGATCAC CAAGCACAAA ACCATTCTGA TACAAACTCA 
GGAAAGTCCC TGGAAATTGA TAATACAGGG CAAACAGTGA CCGTCAATTG TGTTACTCAG 
TCAGAAAATA GTATGGAAAC ATCTGAATGT GAATATTCGG TGAACAGATT CATAGACGAT 
CTCCCTGAGG TATCAACTAG CACTCTACCC CCTGACCTAG ATGAACAATT AAAAAAAACT 
GAGCAAAATA ATCCAGTGCA CCTCTCTTCC AAGTCCACAA ATTCCTTAGC TTCTCCTCTG 
AGTTCTCCAA TGAAACAGAA GCCTCCTATA TCACCCAAAA AACCCAATCT TTCCCTTATT 
ATACCACCCA TAATGCACTT GCCATTTTCC AACGGACTCA AGCTTCAGCA GGTTTATGAA 
GCACAAGAGA CACTAGAGTC AGAGCAAGTG AATGGTGCAT CCTCGACAAA CACAGTTAAT 
TTGCAAGCAA TTCCTTTAGA CCTTGAGGTA GAGGAAGAGA GTGACTCAGA CTTATCAACC 
CCTGTGGTCA AGAGCAGACT ATCTTTCTGC AGCGAGTTAT CATCAAGCAG TTTCCAAGAC 
CTGCAACTTT CTGATTACAT TCTTAATGAG CATGACCTCG GCCTGAGCGA TAAAGACTTT 
GGGCTCTGTG ATGATAAAAG CACATCAGAT GATGGTTCAA GCAGCAGCTC AGGATCCATC 
AGCTTTAAAG AGGATGAACA TGAGGAAAAC GGTGCTGTGT TTGACTCAAT CACTGAAAGC 
ACATCTGACA TTAATGCTAG CGGAGAAGCT GTGGAGGGGA TGGTGACGCC TATTCGCCCT 
CGTACTACAG AGGACCTCTT TGCTGCAATT CATAGGTCAA AAAGGAAGGT CCTGGGCCGT 
GGCGATTCTG AAGATGAACG CTGCCGTAAC CTCCTTCCTT CCCCACCTGT CACTCCAACT 
GGCTCCTGCC CCAGCTTGCC ACGTCAGACC AGCTCCATCC AGCGGAACCT ACGTCGCTCC 
ACCACAAGCA ATGACAGTTT TAAAGCTTTG CTACTCAAGA AGGGCAGCCG CTCAGAGTCC 
AGCTTTCGCA TGTCAGCCGC TGAGATTCTC AAATGCACAG ACCCCCGTTT CCAAAGGGCT 
AATTCAGAAA ATGCACTAGC TGACGGACTG TCCTCTCCGA CAAGCAGCAG GAGAGCACAT 
GAAGAGTGGG CACGGACGGA GGGAGCTTTG CCTCGCTTTA CCACTGGACA AACTATCCCA 
AAATATGGGC GCTCGCGCAC ACCACCTTCC GCAGCCAGCA GCCGGTATAA CAGCCGCAGC 
CGGATTACCA GTAGGCCAAT GACTGCCATC TGTGAACGAG ATGGAGAGGT GGCTGAATCT 
ACAGACTGCT ACTTAAGTAG CGAGAATCAT TTTGTCTTGG CTTTATCCTC CAGCAGCATG 
GTCTGCGCTC AAGGCAGCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467440.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323115.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.

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CloneID	ODa22653
Clone ID Related Accession (Same CDS sequence)	XM_005158823.3 , XM_021467440.1
Accession Version	XM_021467440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGCGGTTTA AAGTCCTAGA CTGCCTATCA 
CAGTCTTGCC TCTCTCTCTG CTGTCATTTG AGATCTTGGA AAACAAAGGC TTCCGATTCA 
CCTAATGAAG AGGATAAACT TGTGTACTCA ATGCCGCCAC AAACCCCTCT GCTGGAGCAT 
GGCTCTGACA TGGACATCCA GAGAAGCTGG ACCAGCTGTC TACCTTTGCC CACTCCCGAG 
GAGAAGATGA GACTGCAGGC CCAATCGGTG GCTACTGATA TAATTCCTAT CAACATTACA 
GGTGAGAACT TTGACCGCCA GGCCAGCTTT CGCCGCTCCA TGGCAAACAC TGATACTATT 
ATACGAAGAC CTAAGAGAGT TCAAAGAAGA AAGACAATAA CAGGAGTGCC TGACAACATC 
CAGACTGAAC TAGCAAATAA AGAACAAGCT GACTCCAGAA TACATTCGAG TATGGCAGAT 
CCATACTCCA CCCTCAGTCG AGTGGGAAGT GTAAACTCCA CCCTGAAACG CTCAGACACC 
CGTGATTCCT GTTGTCAAAC AGAAGAGGTG AAGATTGTTC CTCCCTCAGT ACGCAGAATC 
AGAGCACAAA GAGGCCATGG AATAGCTGCC CAAATGGCCA GTGTCTCCTC CTCAAGCAGC 
ATTTCCACCA TAAGTGACTG TAATGGCATT AGCTACACAC AGCTTAATGA CAGTGATCAG 
AGCTTTCAAA GTTTACCAAG ACAAGGTTCT CGCATCCCGC AACAACACAG TGAAACAAAG 
TACAATAGCT CCCCCTACAG GGCGAATTCT GGTTCTACAA GCCCCTTGTC CTACCATTTT 
AACATGCAGC ATGACTCTTC ATCATCGCTG CATATGCTGT CTGACTCGAG AAGCAAGCTT 
ACTGAAAATC TAAATTACGA AGGCTCTGAT AAAAATAATA CCTCAAGCTT GTTTAGTCTC 
AGTGGTGCTC TACAGAACTC CATTCCTTAT GGATACAAAA GGACAGAGGA CAACCAAATG 
CAATCACCTC ATTCGTCAGT CCAGTTGCTC AATTCTGAGG ATGCCTCATT GTCGGGTGTC 
TCCTCGTGTT ATGAATCCAC AGCGTCCCTC TGTACAGGTA TTCATACAGA GACTGAGTCC 
CAGTGCAGCA CACTTGATGG CAGAAACTGC TGCAGTCCTA ACTGTGTCCG CAGGGATTCC 
AACTTCTCAG AGAGCAGCAA CCAAAGCTGC AGCACACTAA CCACGGACCA ATGGACGTAT 
GAAGTTTCTC CAAAAGACAA TGTTACTTCA AGTTGCTCCT CACCTGTCAG CCATATATAC 
CACGCAGAGG AACATTCTCC CAGCAAGACA GATACAAGCT CATTGTTCTC TGTTGACAAT 
GAAGGTTACT ACACCTCCAT GCGCCTAGAC TCTGGTTTGA AGTCACACAG TCACGGCTGC 
ATCAATAAAG CAGGAGAACA AAAGCACAGT TTGTATGAGT GCAAAGACCA CCACAGTCAA 
AGTGATACTG CAAGTCTGCA TAGCAACCGC TCCCTGACTC GTAGCATCTC TCTGCGAAAA 
GCCAGGAAGC CCCCACTCCC TCCAGAAAGA ACCGACTCTT TAAGGCGTAA ACCCCAGAGG 
AAGAGTCGCC ACAGTGGTTC AGTCCTTAAT GAGCAGTTAA TCTCTAGTCT CCAGCAGTCC 
TTACAACTTA ACCTAAATAC TCAAACTACA TCTTGCTCTG AACAGTCACC CTGTGGTAGG 
TTTGAAGACC CTTGGGTCCT CCGACCGAGA AGTCAGAGTA TCATAAGTGC AGCTAGCAGT 
GGGGTATCTG CACCAGCTGC TGTATGTCCG GTCACACCAA CACACAGTGA CAGCAGCAGT 
CAGCGCTCAG ACTATGCAGA GTCATGGGAC TTCTACATGG ATTGCCCATG TACACGGTCT 
GACCAGAGTC TTTCCTCACA AATCACAAGG TCTGCAAGTG CTGAGGAGAA TCATGGAGTC 
ATAGGTAATG CATCCTACAA CAGTGAATTC CCATCATCCC CCTTCAGCAG TAGTCAGTCA 
AAATTGACCT CATCTCCTGA CAAAGTCCAT CGACTCACGT CACCATCAAG TGGCTACTCA 
AGTCAGTCTA ACACTCCAAC TGCAGGCACT CCAGTTACGT CTCTAATAAG AGCAAAGTCT 
CCTGCGGGGA GACCGAAACC AAAAGTGCCA GAAAGAAAGT CCTCTCTACG ATCTTCTATA 
TCATCCTCTT CTACATCCCT GTCCTCCAAC ACCTCAGATT CCATCAGGAA CATTCCTCCA 
CCTCCACCTT TACCTGACAT GACAACTGCA TTATGTGGGT CGCCCAGTTC TGCTTCTTTC 
TCTCCAACAG CGGCTAAAAT AGACCCAATA CCCTCTTTTA CATCTCTACA GGATGTATCA 
TCTGCCTTGG AAGATGGGCA AAATACACTT TCAGATACAT CTGCAACACC TGAGGAATGT 
GATAGTCCTG ATTTTCCCCC TCCCCATTCA AATGTTTTTG AATCTCTGTC AGGCAATGTA 
GATACCAACA GCCTCCCACC TCCTCCACTT CTGCTTTGCA AACCTTCACT GTGCATTTCA 
TCTCTTCTTC CCCCTCCTCC TCCACCTCCT CCACTATTAC TTGAGACCAG TCTTCAACCA 
GCCACCATTT TGAAGACAGA GAGCAGTCTT ACACCTACAA CAGCTGTAAA ATGTGAAAAA 
GAAGTAAACA TCAGTCACAC TGGAGAGCAA GAAAAGACTC AGAGACCAAT AATCACTGCC 
CAGGCCCTTC AGATGGTGCA GTTGCGACCT GTTAAGCTCA AGCAAATAGA AACTATTTTT 
ACATCTGTCA GTTTTGATCA TTCTGATCAC CAAGCACAAA ACCATTCTGA TACAAACTCA 
GGAAAGTCCC TGGAAATTGA TAATACAGGG CAAACAGTGA CCGTCAATTG TGTTACTCAG 
TCAGAAAATA GTATGGAAAC ATCTGAATGT GAATATTCGG TGAACAGATT CATAGACGAT 
CTCCCTGAGG TATCAACTAG CACTCTACCC CCTGACCTAG ATGAACAATT AAAAAAAACT 
GAGCAAAATA ATCCAGTGCA CCTCTCTTCC AAGTCCACAA ATTCCTTAGC TTCTCCTCTG 
AGTTCTCCAA TGAAACAGAA GCCTCCTATA TCACCCAAAA AACCCAATCT TTCCCTTATT 
ATACCACCCA TAATGCACTT GCCATTTTCC AACGGACTCA AGCTTCAGCA GGTTTATGAA 
GCACAAGAGA CACTAGAGTC AGAGCAAGTG AATGGTGCAT CCTCGACAAA CACAGTTAAT 
TTGCAAGCAA TTCCTTTAGA CCTTGAGGTA GAGGAAGAGA GTGACTCAGA CTTATCAACC 
CCTGTGGTCA AGAGCAGACT ATCTTTCTGC AGCGAGTTAT CATCAAGCAG TTTCCAAGAC 
CTGCAACTTT CTGATTACAT TCTTAATGAG CATGACCTCG GCCTGAGCGA TAAAGACTTT 
GGGCTCTGTG ATGATAAAAG CACATCAGAT GATGGTTCAA GCAGCAGCTC AGGATCCATC 
AGCTTTAAAG AGGATGAACA TGAGGAAAAC GGTGCTGTGT TTGACTCAAT CACTGAAAGC 
ACATCTGACA TTAATGCTAG CGGAGAAGCT GTGGAGGGGA TGGTGACGCC TATTCGCCCT 
CGTACTACAG AGGACCTCTT TGCTGCAATT CATAGGTCAA AAAGGAAGGT CCTGGGCCGT 
GGCGATTCTG AAGATGAACG CTGCCGTAAC CTCCTTCCTT CCCCACCTGT CACTCCAACT 
GGCTCCTGCC CCAGCTTGCC ACGTCAGACC AGCTCCATCC AGCGGAACCT ACGTCGCTCC 
ACCACAAGCA ATGACAGTTT TAAAGCTTTG CTACTCAAGA AGGGCAGCCG CTCAGAGTCC 
AGCTTTCGCA TGTCAGCCGC TGAGATTCTC AAATGCACAG ACCCCCGTTT CCAAAGGGCT 
AATTCAGAAA ATGCACTAGC TGACGGACTG TCCTCTCCGA CAAGCAGCAG GAGAGCACAT 
GAAGAGTGGG CACGGACGGA GGGAGCTTTG CCTCGCTTTA CCACTGGACA AACTATCCCA 
AAATATGGGC GCTCGCGCAC ACCACCTTCC GCAGCCAGCA GCCGGTATAA CAGCCGCAGC 
CGGATTACCA GTAGGCCAAT GACTGCCATC TGTGAACGAG ATGGAGAGGT GGCTGAATCT 
ACAGACTGCT ACTTAAGTAG CGAGAATCAT TTTGTCTTGG CTTTATCCTC CAGCAGCATG 
GTCTGCGCTC AAGGCAGCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323115.1
CDS	171..4754
Translation	MVFIGTTLKSVIKYFKKKAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467440.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467440.1

ORF Insert Sequence:

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGCGGTTTA AAGTCCTAGA CTGCCTATCA 
CAGTCTTGCC TCTCTCTCTG CTGTCATTTG AGATCTTGGA AAACAAAGGC TTCCGATTCA 
CCTAATGAAG AGGATAAACT TGTGTACTCA ATGCCGCCAC AAACCCCTCT GCTGGAGCAT 
GGCTCTGACA TGGACATCCA GAGAAGCTGG ACCAGCTGTC TACCTTTGCC CACTCCCGAG 
GAGAAGATGA GACTGCAGGC CCAATCGGTG GCTACTGATA TAATTCCTAT CAACATTACA 
GGTGAGAACT TTGACCGCCA GGCCAGCTTT CGCCGCTCCA TGGCAAACAC TGATACTATT 
ATACGAAGAC CTAAGAGAGT TCAAAGAAGA AAGACAATAA CAGGAGTGCC TGACAACATC 
CAGACTGAAC TAGCAAATAA AGAACAAGCT GACTCCAGAA TACATTCGAG TATGGCAGAT 
CCATACTCCA CCCTCAGTCG AGTGGGAAGT GTAAACTCCA CCCTGAAACG CTCAGACACC 
CGTGATTCCT GTTGTCAAAC AGAAGAGGTG AAGATTGTTC CTCCCTCAGT ACGCAGAATC 
AGAGCACAAA GAGGCCATGG AATAGCTGCC CAAATGGCCA GTGTCTCCTC CTCAAGCAGC 
ATTTCCACCA TAAGTGACTG TAATGGCATT AGCTACACAC AGCTTAATGA CAGTGATCAG 
AGCTTTCAAA GTTTACCAAG ACAAGGTTCT CGCATCCCGC AACAACACAG TGAAACAAAG 
TACAATAGCT CCCCCTACAG GGCGAATTCT GGTTCTACAA GCCCCTTGTC CTACCATTTT 
AACATGCAGC ATGACTCTTC ATCATCGCTG CATATGCTGT CTGACTCGAG AAGCAAGCTT 
ACTGAAAATC TAAATTACGA AGGCTCTGAT AAAAATAATA CCTCAAGCTT GTTTAGTCTC 
AGTGGTGCTC TACAGAACTC CATTCCTTAT GGATACAAAA GGACAGAGGA CAACCAAATG 
CAATCACCTC ATTCGTCAGT CCAGTTGCTC AATTCTGAGG ATGCCTCATT GTCGGGTGTC 
TCCTCGTGTT ATGAATCCAC AGCGTCCCTC TGTACAGGTA TTCATACAGA GACTGAGTCC 
CAGTGCAGCA CACTTGATGG CAGAAACTGC TGCAGTCCTA ACTGTGTCCG CAGGGATTCC 
AACTTCTCAG AGAGCAGCAA CCAAAGCTGC AGCACACTAA CCACGGACCA ATGGACGTAT 
GAAGTTTCTC CAAAAGACAA TGTTACTTCA AGTTGCTCCT CACCTGTCAG CCATATATAC 
CACGCAGAGG AACATTCTCC CAGCAAGACA GATACAAGCT CATTGTTCTC TGTTGACAAT 
GAAGGTTACT ACACCTCCAT GCGCCTAGAC TCTGGTTTGA AGTCACACAG TCACGGCTGC 
ATCAATAAAG CAGGAGAACA AAAGCACAGT TTGTATGAGT GCAAAGACCA CCACAGTCAA 
AGTGATACTG CAAGTCTGCA TAGCAACCGC TCCCTGACTC GTAGCATCTC TCTGCGAAAA 
GCCAGGAAGC CCCCACTCCC TCCAGAAAGA ACCGACTCTT TAAGGCGTAA ACCCCAGAGG 
AAGAGTCGCC ACAGTGGTTC AGTCCTTAAT GAGCAGTTAA TCTCTAGTCT CCAGCAGTCC 
TTACAACTTA ACCTAAATAC TCAAACTACA TCTTGCTCTG AACAGTCACC CTGTGGTAGG 
TTTGAAGACC CTTGGGTCCT CCGACCGAGA AGTCAGAGTA TCATAAGTGC AGCTAGCAGT 
GGGGTATCTG CACCAGCTGC TGTATGTCCG GTCACACCAA CACACAGTGA CAGCAGCAGT 
CAGCGCTCAG ACTATGCAGA GTCATGGGAC TTCTACATGG ATTGCCCATG TACACGGTCT 
GACCAGAGTC TTTCCTCACA AATCACAAGG TCTGCAAGTG CTGAGGAGAA TCATGGAGTC 
ATAGGTAATG CATCCTACAA CAGTGAATTC CCATCATCCC CCTTCAGCAG TAGTCAGTCA 
AAATTGACCT CATCTCCTGA CAAAGTCCAT CGACTCACGT CACCATCAAG TGGCTACTCA 
AGTCAGTCTA ACACTCCAAC TGCAGGCACT CCAGTTACGT CTCTAATAAG AGCAAAGTCT 
CCTGCGGGGA GACCGAAACC AAAAGTGCCA GAAAGAAAGT CCTCTCTACG ATCTTCTATA 
TCATCCTCTT CTACATCCCT GTCCTCCAAC ACCTCAGATT CCATCAGGAA CATTCCTCCA 
CCTCCACCTT TACCTGACAT GACAACTGCA TTATGTGGGT CGCCCAGTTC TGCTTCTTTC 
TCTCCAACAG CGGCTAAAAT AGACCCAATA CCCTCTTTTA CATCTCTACA GGATGTATCA 
TCTGCCTTGG AAGATGGGCA AAATACACTT TCAGATACAT CTGCAACACC TGAGGAATGT 
GATAGTCCTG ATTTTCCCCC TCCCCATTCA AATGTTTTTG AATCTCTGTC AGGCAATGTA 
GATACCAACA GCCTCCCACC TCCTCCACTT CTGCTTTGCA AACCTTCACT GTGCATTTCA 
TCTCTTCTTC CCCCTCCTCC TCCACCTCCT CCACTATTAC TTGAGACCAG TCTTCAACCA 
GCCACCATTT TGAAGACAGA GAGCAGTCTT ACACCTACAA CAGCTGTAAA ATGTGAAAAA 
GAAGTAAACA TCAGTCACAC TGGAGAGCAA GAAAAGACTC AGAGACCAAT AATCACTGCC 
CAGGCCCTTC AGATGGTGCA GTTGCGACCT GTTAAGCTCA AGCAAATAGA AACTATTTTT 
ACATCTGTCA GTTTTGATCA TTCTGATCAC CAAGCACAAA ACCATTCTGA TACAAACTCA 
GGAAAGTCCC TGGAAATTGA TAATACAGGG CAAACAGTGA CCGTCAATTG TGTTACTCAG 
TCAGAAAATA GTATGGAAAC ATCTGAATGT GAATATTCGG TGAACAGATT CATAGACGAT 
CTCCCTGAGG TATCAACTAG CACTCTACCC CCTGACCTAG ATGAACAATT AAAAAAAACT 
GAGCAAAATA ATCCAGTGCA CCTCTCTTCC AAGTCCACAA ATTCCTTAGC TTCTCCTCTG 
AGTTCTCCAA TGAAACAGAA GCCTCCTATA TCACCCAAAA AACCCAATCT TTCCCTTATT 
ATACCACCCA TAATGCACTT GCCATTTTCC AACGGACTCA AGCTTCAGCA GGTTTATGAA 
GCACAAGAGA CACTAGAGTC AGAGCAAGTG AATGGTGCAT CCTCGACAAA CACAGTTAAT 
TTGCAAGCAA TTCCTTTAGA CCTTGAGGTA GAGGAAGAGA GTGACTCAGA CTTATCAACC 
CCTGTGGTCA AGAGCAGACT ATCTTTCTGC AGCGAGTTAT CATCAAGCAG TTTCCAAGAC 
CTGCAACTTT CTGATTACAT TCTTAATGAG CATGACCTCG GCCTGAGCGA TAAAGACTTT 
GGGCTCTGTG ATGATAAAAG CACATCAGAT GATGGTTCAA GCAGCAGCTC AGGATCCATC 
AGCTTTAAAG AGGATGAACA TGAGGAAAAC GGTGCTGTGT TTGACTCAAT CACTGAAAGC 
ACATCTGACA TTAATGCTAG CGGAGAAGCT GTGGAGGGGA TGGTGACGCC TATTCGCCCT 
CGTACTACAG AGGACCTCTT TGCTGCAATT CATAGGTCAA AAAGGAAGGT CCTGGGCCGT 
GGCGATTCTG AAGATGAACG CTGCCGTAAC CTCCTTCCTT CCCCACCTGT CACTCCAACT 
GGCTCCTGCC CCAGCTTGCC ACGTCAGACC AGCTCCATCC AGCGGAACCT ACGTCGCTCC 
ACCACAAGCA ATGACAGTTT TAAAGCTTTG CTACTCAAGA AGGGCAGCCG CTCAGAGTCC 
AGCTTTCGCA TGTCAGCCGC TGAGATTCTC AAATGCACAG ACCCCCGTTT CCAAAGGGCT 
AATTCAGAAA ATGCACTAGC TGACGGACTG TCCTCTCCGA CAAGCAGCAG GAGAGCACAT 
GAAGAGTGGG CACGGACGGA GGGAGCTTTG CCTCGCTTTA CCACTGGACA AACTATCCCA 
AAATATGGGC GCTCGCGCAC ACCACCTTCC GCAGCCAGCA GCCGGTATAA CAGCCGCAGC 
CGGATTACCA GTAGGCCAAT GACTGCCATC TGTGAACGAG ATGGAGAGGT GGCTGAATCT 
ACAGACTGCT ACTTAAGTAG CGAGAATCAT TTTGTCTTGG CTTTATCCTC CAGCAGCATG 
GTCTGCGCTC AAGGCAGCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_689979.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_695071.6 Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.

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CloneID	ODa22654
Clone ID Related Accession (Same CDS sequence)	XM_689979.8 , XM_021467441.1
Accession Version	XM_689979.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689979.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGGCTTCCG ATTCACCTAA TGAAGAGGAT 
AAACTTGTGT ACTCAATGCC GCCACAAACC CCTCTGCTGG AGCATGGCTC TGACATGGAC 
ATCCAGAGAA GCTGGACCAG CTGTCTACCT TTGCCCACTC CCGAGGAGAA GATGAGACTG 
CAGGCCCAAT CGGTGGCTAC TGATATAATT CCTATCAACA TTACAGGTGA GAACTTTGAC 
CGCCAGGCCA GCTTTCGCCG CTCCATGGCA AACACTGATA CTATTATACG AAGACCTAAG 
AGAGTTCAAA GAAGAAAGAC AATAACAGGA GTGCCTGACA ACATCCAGAC TGAACTAGCA 
AATAAAGAAC AAGCTGACTC CAGAATACAT TCGAGTATGG CAGATCCATA CTCCACCCTC 
AGTCGAGTGG GAAGTGTAAA CTCCACCCTG AAACGCTCAG ACACCCGTGA TTCCTGTTGT 
CAAACAGAAG AGGTGAAGAT TGTTCCTCCC TCAGTACGCA GAATCAGAGC ACAAAGAGGC 
CATGGAATAG CTGCCCAAAT GGCCAGTGTC TCCTCCTCAA GCAGCATTTC CACCATAAGT 
GACTGTAATG GCATTAGCTA CACACAGCTT AATGACAGTG ATCAGAGCTT TCAAAGTTTA 
CCAAGACAAG GTTCTCGCAT CCCGCAACAA CACAGTGAAA CAAAGTACAA TAGCTCCCCC 
TACAGGGCGA ATTCTGGTTC TACAAGCCCC TTGTCCTACC ATTTTAACAT GCAGCATGAC 
TCTTCATCAT CGCTGCATAT GCTGTCTGAC TCGAGAAGCA AGCTTACTGA AAATCTAAAT 
TACGAAGGCT CTGATAAAAA TAATACCTCA AGCTTGTTTA GTCTCAGTGG TGCTCTACAG 
AACTCCATTC CTTATGGATA CAAAAGGACA GAGGACAACC AAATGCAATC ACCTCATTCG 
TCAGTCCAGT TGCTCAATTC TGAGGATGCC TCATTGTCGG GTGTCTCCTC GTGTTATGAA 
TCCACAGCGT CCCTCTGTAC AGGTATTCAT ACAGAGACTG AGTCCCAGTG CAGCACACTT 
GATGGCAGAA ACTGCTGCAG TCCTAACTGT GTCCGCAGGG ATTCCAACTT CTCAGAGAGC 
AGCAACCAAA GCTGCAGCAC ACTAACCACG GACCAATGGA CGTATGAAGT TTCTCCAAAA 
GACAATGTTA CTTCAAGTTG CTCCTCACCT GTCAGCCATA TATACCACGC AGAGGAACAT 
TCTCCCAGCA AGACAGATAC AAGCTCATTG TTCTCTGTTG ACAATGAAGG TTACTACACC 
TCCATGCGCC TAGACTCTGG TTTGAAGTCA CACAGTCACG GCTGCATCAA TAAAGCAGGA 
GAACAAAAGC ACAGTTTGTA TGAGTGCAAA GACCACCACA GTCAAAGTGA TACTGCAAGT 
CTGCATAGCA ACCGCTCCCT GACTCGTAGC ATCTCTCTGC GAAAAGCCAG GAAGCCCCCA 
CTCCCTCCAG AAAGAACCGA CTCTTTAAGG CGTAAACCCC AGAGGAAGAG TCGCCACAGT 
GGTTCAGTCC TTAATGAGCA GTTAATCTCT AGTCTCCAGC AGTCCTTACA ACTTAACCTA 
AATACTCAAA CTACATCTTG CTCTGAACAG TCACCCTGTG GTAGGTTTGA AGACCCTTGG 
GTCCTCCGAC CGAGAAGTCA GAGTATCATA AGTGCAGCTA GCAGTGGGGT ATCTGCACCA 
GCTGCTGTAT GTCCGGTCAC ACCAACACAC AGTGACAGCA GCAGTCAGCG CTCAGACTAT 
GCAGAGTCAT GGGACTTCTA CATGGATTGC CCATGTACAC GGTCTGACCA GAGTCTTTCC 
TCACAAATCA CAAGGTCTGC AAGTGCTGAG GAGAATCATG GAGTCATAGG TAATGCATCC 
TACAACAGTG AATTCCCATC ATCCCCCTTC AGCAGTAGTC AGTCAAAATT GACCTCATCT 
CCTGACAAAG TCCATCGACT CACGTCACCA TCAAGTGGCT ACTCAAGTCA GTCTAACACT 
CCAACTGCAG GCACTCCAGT TACGTCTCTA ATAAGAGCAA AGTCTCCTGC GGGGAGACCG 
AAACCAAAAG TGCCAGAAAG AAAGTCCTCT CTACGATCTT CTATATCATC CTCTTCTACA 
TCCCTGTCCT CCAACACCTC AGATTCCATC AGGAACATTC CTCCACCTCC ACCTTTACCT 
GACATGACAA CTGCATTATG TGGGTCGCCC AGTTCTGCTT CTTTCTCTCC AACAGCGGCT 
AAAATAGACC CAATACCCTC TTTTACATCT CTACAGGATG TATCATCTGC CTTGGAAGAT 
GGGCAAAATA CACTTTCAGA TACATCTGCA ACACCTGAGG AATGTGATAG TCCTGATTTT 
CCCCCTCCCC ATTCAAATGT TTTTGAATCT CTGTCAGGCA ATGTAGATAC CAACAGCCTC 
CCACCTCCTC CACTTCTGCT TTGCAAACCT TCACTGTGCA TTTCATCTCT TCTTCCCCCT 
CCTCCTCCAC CTCCTCCACT ATTACTTGAG ACCAGTCTTC AACCAGCCAC CATTTTGAAG 
ACAGAGAGCA GTCTTACACC TACAACAGCT GTAAAATGTG AAAAAGAAGT AAACATCAGT 
CACACTGGAG AGCAAGAAAA GACTCAGAGA CCAATAATCA CTGCCCAGGC CCTTCAGATG 
GTGCAGTTGC GACCTGTTAA GCTCAAGCAA ATAGAAACTA TTTTTACATC TGTCAGTTTT 
GATCATTCTG ATCACCAAGC ACAAAACCAT TCTGATACAA ACTCAGGAAA GTCCCTGGAA 
ATTGATAATA CAGGGCAAAC AGTGACCGTC AATTGTGTTA CTCAGTCAGA AAATAGTATG 
GAAACATCTG AATGTGAATA TTCGGTGAAC AGATTCATAG ACGATCTCCC TGAGGTATCA 
ACTAGCACTC TACCCCCTGA CCTAGATGAA CAATTAAAAA AAACTGAGCA AAATAATCCA 
GTGCACCTCT CTTCCAAGTC CACAAATTCC TTAGCTTCTC CTCTGAGTTC TCCAATGAAA 
CAGAAGCCTC CTATATCACC CAAAAAACCC AATCTTTCCC TTATTATACC ACCCATAATG 
CACTTGCCAT TTTCCAACGG ACTCAAGCTT CAGCAGGTTT ATGAAGCACA AGAGACACTA 
GAGTCAGAGC AAGTGAATGG TGCATCCTCG ACAAACACAG TTAATTTGCA AGCAATTCCT 
TTAGACCTTG AGGTAGAGGA AGAGAGTGAC TCAGACTTAT CAACCCCTGT GGTCAAGAGC 
AGACTATCTT TCTGCAGCGA GTTATCATCA AGCAGTTTCC AAGACCTGCA ACTTTCTGAT 
TACATTCTTA ATGAGCATGA CCTCGGCCTG AGCGATAAAG ACTTTGGGCT CTGTGATGAT 
AAAAGCACAT CAGATGATGG TTCAAGCAGC AGCTCAGGAT CCATCAGCTT TAAAGAGGAT 
GAACATGAGG AAAACGGTGC TGTGTTTGAC TCAATCACTG AAAGCACATC TGACATTAAT 
GCTAGCGGAG AAGCTGTGGA GGGGATGGTG ACGCCTATTC GCCCTCGTAC TACAGAGGAC 
CTCTTTGCTG CAATTCATAG GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGAAGAT 
GAACGCTGCC GTAACCTCCT TCCTTCCCCA CCTGTCACTC CAACTGGCTC CTGCCCCAGC 
TTGCCACGTC AGACCAGCTC CATCCAGCGG AACCTACGTC GCTCCACCAC AAGCAATGAC 
AGTTTTAAAG CTTTGCTACT CAAGAAGGGC AGCCGCTCAG AGTCCAGCTT TCGCATGTCA 
GCCGCTGAGA TTCTCAAATG CACAGACCCC CGTTTCCAAA GGGCTAATTC AGAAAATGCA 
CTAGCTGACG GACTGTCCTC TCCGACAAGC AGCAGGAGAG CACATGAAGA GTGGGCACGG 
ACGGAGGGAG CTTTGCCTCG CTTTACCACT GGACAAACTA TCCCAAAATA TGGGCGCTCG 
CGCACACCAC CTTCCGCAGC CAGCAGCCGG TATAACAGCC GCAGCCGGAT TACCAGTAGG 
CCAATGACTG CCATCTGTGA ACGAGATGGA GAGGTGGCTG AATCTACAGA CTGCTACTTA 
AGTAGCGAGA ATCATTTTGT CTTGGCTTTA TCCTCCAGCA GCATGGTCTG CGCTCAAGGC 
AGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695071.6
CDS	170..4678
Translation	MVFIGTTLKSVIKYFKKKAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_689979.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689979.8

ORF Insert Sequence:

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGGCTTCCG ATTCACCTAA TGAAGAGGAT 
AAACTTGTGT ACTCAATGCC GCCACAAACC CCTCTGCTGG AGCATGGCTC TGACATGGAC 
ATCCAGAGAA GCTGGACCAG CTGTCTACCT TTGCCCACTC CCGAGGAGAA GATGAGACTG 
CAGGCCCAAT CGGTGGCTAC TGATATAATT CCTATCAACA TTACAGGTGA GAACTTTGAC 
CGCCAGGCCA GCTTTCGCCG CTCCATGGCA AACACTGATA CTATTATACG AAGACCTAAG 
AGAGTTCAAA GAAGAAAGAC AATAACAGGA GTGCCTGACA ACATCCAGAC TGAACTAGCA 
AATAAAGAAC AAGCTGACTC CAGAATACAT TCGAGTATGG CAGATCCATA CTCCACCCTC 
AGTCGAGTGG GAAGTGTAAA CTCCACCCTG AAACGCTCAG ACACCCGTGA TTCCTGTTGT 
CAAACAGAAG AGGTGAAGAT TGTTCCTCCC TCAGTACGCA GAATCAGAGC ACAAAGAGGC 
CATGGAATAG CTGCCCAAAT GGCCAGTGTC TCCTCCTCAA GCAGCATTTC CACCATAAGT 
GACTGTAATG GCATTAGCTA CACACAGCTT AATGACAGTG ATCAGAGCTT TCAAAGTTTA 
CCAAGACAAG GTTCTCGCAT CCCGCAACAA CACAGTGAAA CAAAGTACAA TAGCTCCCCC 
TACAGGGCGA ATTCTGGTTC TACAAGCCCC TTGTCCTACC ATTTTAACAT GCAGCATGAC 
TCTTCATCAT CGCTGCATAT GCTGTCTGAC TCGAGAAGCA AGCTTACTGA AAATCTAAAT 
TACGAAGGCT CTGATAAAAA TAATACCTCA AGCTTGTTTA GTCTCAGTGG TGCTCTACAG 
AACTCCATTC CTTATGGATA CAAAAGGACA GAGGACAACC AAATGCAATC ACCTCATTCG 
TCAGTCCAGT TGCTCAATTC TGAGGATGCC TCATTGTCGG GTGTCTCCTC GTGTTATGAA 
TCCACAGCGT CCCTCTGTAC AGGTATTCAT ACAGAGACTG AGTCCCAGTG CAGCACACTT 
GATGGCAGAA ACTGCTGCAG TCCTAACTGT GTCCGCAGGG ATTCCAACTT CTCAGAGAGC 
AGCAACCAAA GCTGCAGCAC ACTAACCACG GACCAATGGA CGTATGAAGT TTCTCCAAAA 
GACAATGTTA CTTCAAGTTG CTCCTCACCT GTCAGCCATA TATACCACGC AGAGGAACAT 
TCTCCCAGCA AGACAGATAC AAGCTCATTG TTCTCTGTTG ACAATGAAGG TTACTACACC 
TCCATGCGCC TAGACTCTGG TTTGAAGTCA CACAGTCACG GCTGCATCAA TAAAGCAGGA 
GAACAAAAGC ACAGTTTGTA TGAGTGCAAA GACCACCACA GTCAAAGTGA TACTGCAAGT 
CTGCATAGCA ACCGCTCCCT GACTCGTAGC ATCTCTCTGC GAAAAGCCAG GAAGCCCCCA 
CTCCCTCCAG AAAGAACCGA CTCTTTAAGG CGTAAACCCC AGAGGAAGAG TCGCCACAGT 
GGTTCAGTCC TTAATGAGCA GTTAATCTCT AGTCTCCAGC AGTCCTTACA ACTTAACCTA 
AATACTCAAA CTACATCTTG CTCTGAACAG TCACCCTGTG GTAGGTTTGA AGACCCTTGG 
GTCCTCCGAC CGAGAAGTCA GAGTATCATA AGTGCAGCTA GCAGTGGGGT ATCTGCACCA 
GCTGCTGTAT GTCCGGTCAC ACCAACACAC AGTGACAGCA GCAGTCAGCG CTCAGACTAT 
GCAGAGTCAT GGGACTTCTA CATGGATTGC CCATGTACAC GGTCTGACCA GAGTCTTTCC 
TCACAAATCA CAAGGTCTGC AAGTGCTGAG GAGAATCATG GAGTCATAGG TAATGCATCC 
TACAACAGTG AATTCCCATC ATCCCCCTTC AGCAGTAGTC AGTCAAAATT GACCTCATCT 
CCTGACAAAG TCCATCGACT CACGTCACCA TCAAGTGGCT ACTCAAGTCA GTCTAACACT 
CCAACTGCAG GCACTCCAGT TACGTCTCTA ATAAGAGCAA AGTCTCCTGC GGGGAGACCG 
AAACCAAAAG TGCCAGAAAG AAAGTCCTCT CTACGATCTT CTATATCATC CTCTTCTACA 
TCCCTGTCCT CCAACACCTC AGATTCCATC AGGAACATTC CTCCACCTCC ACCTTTACCT 
GACATGACAA CTGCATTATG TGGGTCGCCC AGTTCTGCTT CTTTCTCTCC AACAGCGGCT 
AAAATAGACC CAATACCCTC TTTTACATCT CTACAGGATG TATCATCTGC CTTGGAAGAT 
GGGCAAAATA CACTTTCAGA TACATCTGCA ACACCTGAGG AATGTGATAG TCCTGATTTT 
CCCCCTCCCC ATTCAAATGT TTTTGAATCT CTGTCAGGCA ATGTAGATAC CAACAGCCTC 
CCACCTCCTC CACTTCTGCT TTGCAAACCT TCACTGTGCA TTTCATCTCT TCTTCCCCCT 
CCTCCTCCAC CTCCTCCACT ATTACTTGAG ACCAGTCTTC AACCAGCCAC CATTTTGAAG 
ACAGAGAGCA GTCTTACACC TACAACAGCT GTAAAATGTG AAAAAGAAGT AAACATCAGT 
CACACTGGAG AGCAAGAAAA GACTCAGAGA CCAATAATCA CTGCCCAGGC CCTTCAGATG 
GTGCAGTTGC GACCTGTTAA GCTCAAGCAA ATAGAAACTA TTTTTACATC TGTCAGTTTT 
GATCATTCTG ATCACCAAGC ACAAAACCAT TCTGATACAA ACTCAGGAAA GTCCCTGGAA 
ATTGATAATA CAGGGCAAAC AGTGACCGTC AATTGTGTTA CTCAGTCAGA AAATAGTATG 
GAAACATCTG AATGTGAATA TTCGGTGAAC AGATTCATAG ACGATCTCCC TGAGGTATCA 
ACTAGCACTC TACCCCCTGA CCTAGATGAA CAATTAAAAA AAACTGAGCA AAATAATCCA 
GTGCACCTCT CTTCCAAGTC CACAAATTCC TTAGCTTCTC CTCTGAGTTC TCCAATGAAA 
CAGAAGCCTC CTATATCACC CAAAAAACCC AATCTTTCCC TTATTATACC ACCCATAATG 
CACTTGCCAT TTTCCAACGG ACTCAAGCTT CAGCAGGTTT ATGAAGCACA AGAGACACTA 
GAGTCAGAGC AAGTGAATGG TGCATCCTCG ACAAACACAG TTAATTTGCA AGCAATTCCT 
TTAGACCTTG AGGTAGAGGA AGAGAGTGAC TCAGACTTAT CAACCCCTGT GGTCAAGAGC 
AGACTATCTT TCTGCAGCGA GTTATCATCA AGCAGTTTCC AAGACCTGCA ACTTTCTGAT 
TACATTCTTA ATGAGCATGA CCTCGGCCTG AGCGATAAAG ACTTTGGGCT CTGTGATGAT 
AAAAGCACAT CAGATGATGG TTCAAGCAGC AGCTCAGGAT CCATCAGCTT TAAAGAGGAT 
GAACATGAGG AAAACGGTGC TGTGTTTGAC TCAATCACTG AAAGCACATC TGACATTAAT 
GCTAGCGGAG AAGCTGTGGA GGGGATGGTG ACGCCTATTC GCCCTCGTAC TACAGAGGAC 
CTCTTTGCTG CAATTCATAG GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGAAGAT 
GAACGCTGCC GTAACCTCCT TCCTTCCCCA CCTGTCACTC CAACTGGCTC CTGCCCCAGC 
TTGCCACGTC AGACCAGCTC CATCCAGCGG AACCTACGTC GCTCCACCAC AAGCAATGAC 
AGTTTTAAAG CTTTGCTACT CAAGAAGGGC AGCCGCTCAG AGTCCAGCTT TCGCATGTCA 
GCCGCTGAGA TTCTCAAATG CACAGACCCC CGTTTCCAAA GGGCTAATTC AGAAAATGCA 
CTAGCTGACG GACTGTCCTC TCCGACAAGC AGCAGGAGAG CACATGAAGA GTGGGCACGG 
ACGGAGGGAG CTTTGCCTCG CTTTACCACT GGACAAACTA TCCCAAAATA TGGGCGCTCG 
CGCACACCAC CTTCCGCAGC CAGCAGCCGG TATAACAGCC GCAGCCGGAT TACCAGTAGG 
CCAATGACTG CCATCTGTGA ACGAGATGGA GAGGTGGCTG AATCTACAGA CTGCTACTTA 
AGTAGCGAGA ATCATTTTGT CTTGGCTTTA TCCTCCAGCA GCATGGTCTG CGCTCAAGGC 
AGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467441.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323116.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa22654
Clone ID Related Accession (Same CDS sequence)	XM_689979.8 , XM_021467441.1
Accession Version	XM_021467441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGGCTTCCG ATTCACCTAA TGAAGAGGAT 
AAACTTGTGT ACTCAATGCC GCCACAAACC CCTCTGCTGG AGCATGGCTC TGACATGGAC 
ATCCAGAGAA GCTGGACCAG CTGTCTACCT TTGCCCACTC CCGAGGAGAA GATGAGACTG 
CAGGCCCAAT CGGTGGCTAC TGATATAATT CCTATCAACA TTACAGGTGA GAACTTTGAC 
CGCCAGGCCA GCTTTCGCCG CTCCATGGCA AACACTGATA CTATTATACG AAGACCTAAG 
AGAGTTCAAA GAAGAAAGAC AATAACAGGA GTGCCTGACA ACATCCAGAC TGAACTAGCA 
AATAAAGAAC AAGCTGACTC CAGAATACAT TCGAGTATGG CAGATCCATA CTCCACCCTC 
AGTCGAGTGG GAAGTGTAAA CTCCACCCTG AAACGCTCAG ACACCCGTGA TTCCTGTTGT 
CAAACAGAAG AGGTGAAGAT TGTTCCTCCC TCAGTACGCA GAATCAGAGC ACAAAGAGGC 
CATGGAATAG CTGCCCAAAT GGCCAGTGTC TCCTCCTCAA GCAGCATTTC CACCATAAGT 
GACTGTAATG GCATTAGCTA CACACAGCTT AATGACAGTG ATCAGAGCTT TCAAAGTTTA 
CCAAGACAAG GTTCTCGCAT CCCGCAACAA CACAGTGAAA CAAAGTACAA TAGCTCCCCC 
TACAGGGCGA ATTCTGGTTC TACAAGCCCC TTGTCCTACC ATTTTAACAT GCAGCATGAC 
TCTTCATCAT CGCTGCATAT GCTGTCTGAC TCGAGAAGCA AGCTTACTGA AAATCTAAAT 
TACGAAGGCT CTGATAAAAA TAATACCTCA AGCTTGTTTA GTCTCAGTGG TGCTCTACAG 
AACTCCATTC CTTATGGATA CAAAAGGACA GAGGACAACC AAATGCAATC ACCTCATTCG 
TCAGTCCAGT TGCTCAATTC TGAGGATGCC TCATTGTCGG GTGTCTCCTC GTGTTATGAA 
TCCACAGCGT CCCTCTGTAC AGGTATTCAT ACAGAGACTG AGTCCCAGTG CAGCACACTT 
GATGGCAGAA ACTGCTGCAG TCCTAACTGT GTCCGCAGGG ATTCCAACTT CTCAGAGAGC 
AGCAACCAAA GCTGCAGCAC ACTAACCACG GACCAATGGA CGTATGAAGT TTCTCCAAAA 
GACAATGTTA CTTCAAGTTG CTCCTCACCT GTCAGCCATA TATACCACGC AGAGGAACAT 
TCTCCCAGCA AGACAGATAC AAGCTCATTG TTCTCTGTTG ACAATGAAGG TTACTACACC 
TCCATGCGCC TAGACTCTGG TTTGAAGTCA CACAGTCACG GCTGCATCAA TAAAGCAGGA 
GAACAAAAGC ACAGTTTGTA TGAGTGCAAA GACCACCACA GTCAAAGTGA TACTGCAAGT 
CTGCATAGCA ACCGCTCCCT GACTCGTAGC ATCTCTCTGC GAAAAGCCAG GAAGCCCCCA 
CTCCCTCCAG AAAGAACCGA CTCTTTAAGG CGTAAACCCC AGAGGAAGAG TCGCCACAGT 
GGTTCAGTCC TTAATGAGCA GTTAATCTCT AGTCTCCAGC AGTCCTTACA ACTTAACCTA 
AATACTCAAA CTACATCTTG CTCTGAACAG TCACCCTGTG GTAGGTTTGA AGACCCTTGG 
GTCCTCCGAC CGAGAAGTCA GAGTATCATA AGTGCAGCTA GCAGTGGGGT ATCTGCACCA 
GCTGCTGTAT GTCCGGTCAC ACCAACACAC AGTGACAGCA GCAGTCAGCG CTCAGACTAT 
GCAGAGTCAT GGGACTTCTA CATGGATTGC CCATGTACAC GGTCTGACCA GAGTCTTTCC 
TCACAAATCA CAAGGTCTGC AAGTGCTGAG GAGAATCATG GAGTCATAGG TAATGCATCC 
TACAACAGTG AATTCCCATC ATCCCCCTTC AGCAGTAGTC AGTCAAAATT GACCTCATCT 
CCTGACAAAG TCCATCGACT CACGTCACCA TCAAGTGGCT ACTCAAGTCA GTCTAACACT 
CCAACTGCAG GCACTCCAGT TACGTCTCTA ATAAGAGCAA AGTCTCCTGC GGGGAGACCG 
AAACCAAAAG TGCCAGAAAG AAAGTCCTCT CTACGATCTT CTATATCATC CTCTTCTACA 
TCCCTGTCCT CCAACACCTC AGATTCCATC AGGAACATTC CTCCACCTCC ACCTTTACCT 
GACATGACAA CTGCATTATG TGGGTCGCCC AGTTCTGCTT CTTTCTCTCC AACAGCGGCT 
AAAATAGACC CAATACCCTC TTTTACATCT CTACAGGATG TATCATCTGC CTTGGAAGAT 
GGGCAAAATA CACTTTCAGA TACATCTGCA ACACCTGAGG AATGTGATAG TCCTGATTTT 
CCCCCTCCCC ATTCAAATGT TTTTGAATCT CTGTCAGGCA ATGTAGATAC CAACAGCCTC 
CCACCTCCTC CACTTCTGCT TTGCAAACCT TCACTGTGCA TTTCATCTCT TCTTCCCCCT 
CCTCCTCCAC CTCCTCCACT ATTACTTGAG ACCAGTCTTC AACCAGCCAC CATTTTGAAG 
ACAGAGAGCA GTCTTACACC TACAACAGCT GTAAAATGTG AAAAAGAAGT AAACATCAGT 
CACACTGGAG AGCAAGAAAA GACTCAGAGA CCAATAATCA CTGCCCAGGC CCTTCAGATG 
GTGCAGTTGC GACCTGTTAA GCTCAAGCAA ATAGAAACTA TTTTTACATC TGTCAGTTTT 
GATCATTCTG ATCACCAAGC ACAAAACCAT TCTGATACAA ACTCAGGAAA GTCCCTGGAA 
ATTGATAATA CAGGGCAAAC AGTGACCGTC AATTGTGTTA CTCAGTCAGA AAATAGTATG 
GAAACATCTG AATGTGAATA TTCGGTGAAC AGATTCATAG ACGATCTCCC TGAGGTATCA 
ACTAGCACTC TACCCCCTGA CCTAGATGAA CAATTAAAAA AAACTGAGCA AAATAATCCA 
GTGCACCTCT CTTCCAAGTC CACAAATTCC TTAGCTTCTC CTCTGAGTTC TCCAATGAAA 
CAGAAGCCTC CTATATCACC CAAAAAACCC AATCTTTCCC TTATTATACC ACCCATAATG 
CACTTGCCAT TTTCCAACGG ACTCAAGCTT CAGCAGGTTT ATGAAGCACA AGAGACACTA 
GAGTCAGAGC AAGTGAATGG TGCATCCTCG ACAAACACAG TTAATTTGCA AGCAATTCCT 
TTAGACCTTG AGGTAGAGGA AGAGAGTGAC TCAGACTTAT CAACCCCTGT GGTCAAGAGC 
AGACTATCTT TCTGCAGCGA GTTATCATCA AGCAGTTTCC AAGACCTGCA ACTTTCTGAT 
TACATTCTTA ATGAGCATGA CCTCGGCCTG AGCGATAAAG ACTTTGGGCT CTGTGATGAT 
AAAAGCACAT CAGATGATGG TTCAAGCAGC AGCTCAGGAT CCATCAGCTT TAAAGAGGAT 
GAACATGAGG AAAACGGTGC TGTGTTTGAC TCAATCACTG AAAGCACATC TGACATTAAT 
GCTAGCGGAG AAGCTGTGGA GGGGATGGTG ACGCCTATTC GCCCTCGTAC TACAGAGGAC 
CTCTTTGCTG CAATTCATAG GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGAAGAT 
GAACGCTGCC GTAACCTCCT TCCTTCCCCA CCTGTCACTC CAACTGGCTC CTGCCCCAGC 
TTGCCACGTC AGACCAGCTC CATCCAGCGG AACCTACGTC GCTCCACCAC AAGCAATGAC 
AGTTTTAAAG CTTTGCTACT CAAGAAGGGC AGCCGCTCAG AGTCCAGCTT TCGCATGTCA 
GCCGCTGAGA TTCTCAAATG CACAGACCCC CGTTTCCAAA GGGCTAATTC AGAAAATGCA 
CTAGCTGACG GACTGTCCTC TCCGACAAGC AGCAGGAGAG CACATGAAGA GTGGGCACGG 
ACGGAGGGAG CTTTGCCTCG CTTTACCACT GGACAAACTA TCCCAAAATA TGGGCGCTCG 
CGCACACCAC CTTCCGCAGC CAGCAGCCGG TATAACAGCC GCAGCCGGAT TACCAGTAGG 
CCAATGACTG CCATCTGTGA ACGAGATGGA GAGGTGGCTG AATCTACAGA CTGCTACTTA 
AGTAGCGAGA ATCATTTTGT CTTGGCTTTA TCCTCCAGCA GCATGGTCTG CGCTCAAGGC 
AGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323116.1
CDS	171..4679
Translation	MVFIGTTLKSVIKYFKKKAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467441.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467441.1

ORF Insert Sequence:

ATGGTGTTCA TCGGGACCAC TCTTAAATCT GTCATTAAGT ACTTTAAAAA GAAGGCGGTG 
TCCAGTCTTG ATGAGGAGAG CAAATGGTCG GTGCACTACA CAGCCCCCTG GCACCACCAA 
GAGAACGTCT TCCTGCCTGG GTCCAGACCA GCATGTGTTG AGGATCTTCA CCGTCAGGCC 
AAAGTCAACC TGAAAACAGT CCTCAGAGAG TGTGACAAGC TCAGAAAAGA TGGCTTTCGC 
AGTTCTCAGT ATTACTCCCA AAGCCCAGCA TTCTCTGCAA CCAGCCTTTG TGAAAGTGTA 
CATCTGGATG AAGAGAAGAC AAAGAAAAAG AAGGCTTCCG ATTCACCTAA TGAAGAGGAT 
AAACTTGTGT ACTCAATGCC GCCACAAACC CCTCTGCTGG AGCATGGCTC TGACATGGAC 
ATCCAGAGAA GCTGGACCAG CTGTCTACCT TTGCCCACTC CCGAGGAGAA GATGAGACTG 
CAGGCCCAAT CGGTGGCTAC TGATATAATT CCTATCAACA TTACAGGTGA GAACTTTGAC 
CGCCAGGCCA GCTTTCGCCG CTCCATGGCA AACACTGATA CTATTATACG AAGACCTAAG 
AGAGTTCAAA GAAGAAAGAC AATAACAGGA GTGCCTGACA ACATCCAGAC TGAACTAGCA 
AATAAAGAAC AAGCTGACTC CAGAATACAT TCGAGTATGG CAGATCCATA CTCCACCCTC 
AGTCGAGTGG GAAGTGTAAA CTCCACCCTG AAACGCTCAG ACACCCGTGA TTCCTGTTGT 
CAAACAGAAG AGGTGAAGAT TGTTCCTCCC TCAGTACGCA GAATCAGAGC ACAAAGAGGC 
CATGGAATAG CTGCCCAAAT GGCCAGTGTC TCCTCCTCAA GCAGCATTTC CACCATAAGT 
GACTGTAATG GCATTAGCTA CACACAGCTT AATGACAGTG ATCAGAGCTT TCAAAGTTTA 
CCAAGACAAG GTTCTCGCAT CCCGCAACAA CACAGTGAAA CAAAGTACAA TAGCTCCCCC 
TACAGGGCGA ATTCTGGTTC TACAAGCCCC TTGTCCTACC ATTTTAACAT GCAGCATGAC 
TCTTCATCAT CGCTGCATAT GCTGTCTGAC TCGAGAAGCA AGCTTACTGA AAATCTAAAT 
TACGAAGGCT CTGATAAAAA TAATACCTCA AGCTTGTTTA GTCTCAGTGG TGCTCTACAG 
AACTCCATTC CTTATGGATA CAAAAGGACA GAGGACAACC AAATGCAATC ACCTCATTCG 
TCAGTCCAGT TGCTCAATTC TGAGGATGCC TCATTGTCGG GTGTCTCCTC GTGTTATGAA 
TCCACAGCGT CCCTCTGTAC AGGTATTCAT ACAGAGACTG AGTCCCAGTG CAGCACACTT 
GATGGCAGAA ACTGCTGCAG TCCTAACTGT GTCCGCAGGG ATTCCAACTT CTCAGAGAGC 
AGCAACCAAA GCTGCAGCAC ACTAACCACG GACCAATGGA CGTATGAAGT TTCTCCAAAA 
GACAATGTTA CTTCAAGTTG CTCCTCACCT GTCAGCCATA TATACCACGC AGAGGAACAT 
TCTCCCAGCA AGACAGATAC AAGCTCATTG TTCTCTGTTG ACAATGAAGG TTACTACACC 
TCCATGCGCC TAGACTCTGG TTTGAAGTCA CACAGTCACG GCTGCATCAA TAAAGCAGGA 
GAACAAAAGC ACAGTTTGTA TGAGTGCAAA GACCACCACA GTCAAAGTGA TACTGCAAGT 
CTGCATAGCA ACCGCTCCCT GACTCGTAGC ATCTCTCTGC GAAAAGCCAG GAAGCCCCCA 
CTCCCTCCAG AAAGAACCGA CTCTTTAAGG CGTAAACCCC AGAGGAAGAG TCGCCACAGT 
GGTTCAGTCC TTAATGAGCA GTTAATCTCT AGTCTCCAGC AGTCCTTACA ACTTAACCTA 
AATACTCAAA CTACATCTTG CTCTGAACAG TCACCCTGTG GTAGGTTTGA AGACCCTTGG 
GTCCTCCGAC CGAGAAGTCA GAGTATCATA AGTGCAGCTA GCAGTGGGGT ATCTGCACCA 
GCTGCTGTAT GTCCGGTCAC ACCAACACAC AGTGACAGCA GCAGTCAGCG CTCAGACTAT 
GCAGAGTCAT GGGACTTCTA CATGGATTGC CCATGTACAC GGTCTGACCA GAGTCTTTCC 
TCACAAATCA CAAGGTCTGC AAGTGCTGAG GAGAATCATG GAGTCATAGG TAATGCATCC 
TACAACAGTG AATTCCCATC ATCCCCCTTC AGCAGTAGTC AGTCAAAATT GACCTCATCT 
CCTGACAAAG TCCATCGACT CACGTCACCA TCAAGTGGCT ACTCAAGTCA GTCTAACACT 
CCAACTGCAG GCACTCCAGT TACGTCTCTA ATAAGAGCAA AGTCTCCTGC GGGGAGACCG 
AAACCAAAAG TGCCAGAAAG AAAGTCCTCT CTACGATCTT CTATATCATC CTCTTCTACA 
TCCCTGTCCT CCAACACCTC AGATTCCATC AGGAACATTC CTCCACCTCC ACCTTTACCT 
GACATGACAA CTGCATTATG TGGGTCGCCC AGTTCTGCTT CTTTCTCTCC AACAGCGGCT 
AAAATAGACC CAATACCCTC TTTTACATCT CTACAGGATG TATCATCTGC CTTGGAAGAT 
GGGCAAAATA CACTTTCAGA TACATCTGCA ACACCTGAGG AATGTGATAG TCCTGATTTT 
CCCCCTCCCC ATTCAAATGT TTTTGAATCT CTGTCAGGCA ATGTAGATAC CAACAGCCTC 
CCACCTCCTC CACTTCTGCT TTGCAAACCT TCACTGTGCA TTTCATCTCT TCTTCCCCCT 
CCTCCTCCAC CTCCTCCACT ATTACTTGAG ACCAGTCTTC AACCAGCCAC CATTTTGAAG 
ACAGAGAGCA GTCTTACACC TACAACAGCT GTAAAATGTG AAAAAGAAGT AAACATCAGT 
CACACTGGAG AGCAAGAAAA GACTCAGAGA CCAATAATCA CTGCCCAGGC CCTTCAGATG 
GTGCAGTTGC GACCTGTTAA GCTCAAGCAA ATAGAAACTA TTTTTACATC TGTCAGTTTT 
GATCATTCTG ATCACCAAGC ACAAAACCAT TCTGATACAA ACTCAGGAAA GTCCCTGGAA 
ATTGATAATA CAGGGCAAAC AGTGACCGTC AATTGTGTTA CTCAGTCAGA AAATAGTATG 
GAAACATCTG AATGTGAATA TTCGGTGAAC AGATTCATAG ACGATCTCCC TGAGGTATCA 
ACTAGCACTC TACCCCCTGA CCTAGATGAA CAATTAAAAA AAACTGAGCA AAATAATCCA 
GTGCACCTCT CTTCCAAGTC CACAAATTCC TTAGCTTCTC CTCTGAGTTC TCCAATGAAA 
CAGAAGCCTC CTATATCACC CAAAAAACCC AATCTTTCCC TTATTATACC ACCCATAATG 
CACTTGCCAT TTTCCAACGG ACTCAAGCTT CAGCAGGTTT ATGAAGCACA AGAGACACTA 
GAGTCAGAGC AAGTGAATGG TGCATCCTCG ACAAACACAG TTAATTTGCA AGCAATTCCT 
TTAGACCTTG AGGTAGAGGA AGAGAGTGAC TCAGACTTAT CAACCCCTGT GGTCAAGAGC 
AGACTATCTT TCTGCAGCGA GTTATCATCA AGCAGTTTCC AAGACCTGCA ACTTTCTGAT 
TACATTCTTA ATGAGCATGA CCTCGGCCTG AGCGATAAAG ACTTTGGGCT CTGTGATGAT 
AAAAGCACAT CAGATGATGG TTCAAGCAGC AGCTCAGGAT CCATCAGCTT TAAAGAGGAT 
GAACATGAGG AAAACGGTGC TGTGTTTGAC TCAATCACTG AAAGCACATC TGACATTAAT 
GCTAGCGGAG AAGCTGTGGA GGGGATGGTG ACGCCTATTC GCCCTCGTAC TACAGAGGAC 
CTCTTTGCTG CAATTCATAG GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGAAGAT 
GAACGCTGCC GTAACCTCCT TCCTTCCCCA CCTGTCACTC CAACTGGCTC CTGCCCCAGC 
TTGCCACGTC AGACCAGCTC CATCCAGCGG AACCTACGTC GCTCCACCAC AAGCAATGAC 
AGTTTTAAAG CTTTGCTACT CAAGAAGGGC AGCCGCTCAG AGTCCAGCTT TCGCATGTCA 
GCCGCTGAGA TTCTCAAATG CACAGACCCC CGTTTCCAAA GGGCTAATTC AGAAAATGCA 
CTAGCTGACG GACTGTCCTC TCCGACAAGC AGCAGGAGAG CACATGAAGA GTGGGCACGG 
ACGGAGGGAG CTTTGCCTCG CTTTACCACT GGACAAACTA TCCCAAAATA TGGGCGCTCG 
CGCACACCAC CTTCCGCAGC CAGCAGCCGG TATAACAGCC GCAGCCGGAT TACCAGTAGG 
CCAATGACTG CCATCTGTGA ACGAGATGGA GAGGTGGCTG AATCTACAGA CTGCTACTTA 
AGTAGCGAGA ATCATTTTGT CTTGGCTTTA TCCTCCAGCA GCATGGTCTG CGCTCAAGGC 
AGCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_005158824.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158881.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa22655
Clone ID Related Accession (Same CDS sequence)	XM_005158824.3 , XM_021467442.1
Accession Version	XM_005158824.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGTCTA CGTAGACAGA ACATGAACAT CCAATTACAG 
GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158881.1
CDS	265..4560
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHSLRRQNMNIQLQVKKEGPGPWRF

Target ORF information:

RefSeq Version	XM_005158824.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158824.3

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGTCTA CGTAGACAGA ACATGAACAT CCAATTACAG 
GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467442.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323117.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.

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CloneID	ODa22655
Clone ID Related Accession (Same CDS sequence)	XM_005158824.3 , XM_021467442.1
Accession Version	XM_021467442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGTCTA CGTAGACAGA ACATGAACAT CCAATTACAG 
GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323117.1
CDS	292..4587
Translation	MIAYVDCLSTEPAGKCWNRTYYEDEDELLPLYTRKLNLQPKTEGSLRRRLLTSKIHQQQDALWAPRHWTGPPTRKGTPLQGQPNYTFSPSIHDWRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHSLRRQNMNIQLQVKKEGPGPWRF

Target ORF information:

RefSeq Version	XM_021467442.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467442.1

ORF Insert Sequence:

ATGATTGCCT ACGTAGACTG CCTTTCCACC GAACCAGCTG GGAAATGCTG GAATAGGACA 
TACTATGAGG ATGAGGATGA ACTTCTTCCT CTTTATACCC GCAAACTAAA CCTCCAACCC 
AAGACAGAGG GCAGTCTTCG TCGAAGACTC CTCACCTCTA AAATTCACCA GCAGCAGGAT 
GCTTTATGGG CACCTAGACA TTGGACGGGA CCACCAACTC GAAAGGGTAC CCCCCTGCAG 
GGTCAGCCTA ACTACACCTT TAGTCCATCC ATCCACGACT GGAGAGCGGT GTCCAGTCTT 
GATGAGGAGA GCAAATGGTC GGTGCACTAC ACAGCCCCCT GGCACCACCA AGAGAACGTC 
TTCCTGCCTG GGTCCAGACC AGCATGTGTT GAGGATCTTC ACCGTCAGGC CAAAGTCAAC 
CTGAAAACAG TCCTCAGAGA GTGTGACAAG CTCAGAAAAG ATGGCTTTCG CAGTTCTCAG 
TATTACTCCC AAAGCCCAGC ATTCTCTGCA ACCAGCCTTT GTGAAAGTGT ACATCTGGAT 
GAAGAGAAGA CAAAGAAAAA GAAGCGGTTT AAAGTCCTAG ACTGCCTATC ACAGTCTTGC 
CTCTCTCTCT GCTGTCATTT GAGATCTTGG AAAACAAAGG CTTCCGATTC ACCTAATGAA 
GAGGATAAAC TTGTGTACTC AATGCCGCCA CAAACCCCTC TGCTGGAGCA TGGCTCTGAC 
ATGGACATCC AGAGAAGCTG GACCAGCTGT CTACCTTTGC CCACTCCCGA GGAGAAGATG 
AGACTGCAGG CCCAATCGGT GGCTACTGAT ATAATTCCTA TCAACATTAC AGGTGAGAAC 
TTTGACCGCC AGGCCAGCTT TCGCCGCTCC ATGGCAAACA CTGATACTAT TATACGAAGA 
CCTAAGAGAG TTCAAAGAAG AAAGACAATA ACAGGAGTGC CTGACAACAT CCAGACTGAA 
CTAGCAAATA AAGAACAAGC TGACTCCAGA ATACATTCGA GTATGGCAGA TCCATACTCC 
ACCCTCAGTC GAGTGGGAAG TGTAAACTCC ACCCTGAAAC GCTCAGACAC CCGTGATTCC 
TGTTGTCAAA CAGAAGAGGT GAAGATTGTT CCTCCCTCAG TACGCAGAAT CAGAGCACAA 
AGAGGCCATG GAATAGCTGC CCAAATGGCC AGTGTCTCCT CCTCAAGCAG CATTTCCACC 
ATAAGTGACT GTAATGGCAT TAGCTACACA CAGCTTAATG ACAGTGATCA GAGCTTTCAA 
AGTTTACCAA GACAAGGTTC TCGCATCCCG CAACAACACA GTGAAACAAA GTACAATAGC 
TCCCCCTACA GGGCGAATTC TGGTTCTACA AGCCCCTTGT CCTACCATTT TAACATGCAG 
CATGACTCTT CATCATCGCT GCATATGCTG TCTGACTCGA GAAGCAAGCT TACTGAAAAT 
CTAAATTACG AAGGCTCTGA TAAAAATAAT ACCTCAAGCT TGTTTAGTCT CAGTGGTGCT 
CTACAGAACT CCATTCCTTA TGGATACAAA AGGACAGAGG ACAACCAAAT GCAATCACCT 
CATTCGTCAG TCCAGTTGCT CAATTCTGAG GATGCCTCAT TGTCGGGTGT CTCCTCGTGT 
TATGAATCCA CAGCGTCCCT CTGTACAGGT ATTCATACAG AGACTGAGTC CCAGTGCAGC 
ACACTTGATG GCAGAAACTG CTGCAGTCCT AACTGTGTCC GCAGGGATTC CAACTTCTCA 
GAGAGCAGCA ACCAAAGCTG CAGCACACTA ACCACGGACC AATGGACGTA TGAAGTTTCT 
CCAAAAGACA ATGTTACTTC AAGTTGCTCC TCACCTGTCA GCCATATATA CCACGCAGAG 
GAACATTCTC CCAGCAAGAC AGATACAAGC TCATTGTTCT CTGTTGACAA TGAAGGTTAC 
TACACCTCCA TGCGCCTAGA CTCTGGTTTG AAGTCACACA GTCACGGCTG CATCAATAAA 
GCAGGAGAAC AAAAGCACAG TTTGTATGAG TGCAAAGACC ACCACAGTCA AAGTGATACT 
GCAAGTCTGC ATAGCAACCG CTCCCTGACT CGTAGCATCT CTCTGCGAAA AGCCAGGAAG 
CCCCCACTCC CTCCAGAAAG AACCGACTCT TTAAGGCGTA AACCCCAGAG GAAGAGTCGC 
CACAGTGGTT CAGTCCTTAA TGAGCAGTTA ATCTCTAGTC TCCAGCAGTC CTTACAACTT 
AACCTAAATA CTCAAACTAC ATCTTGCTCT GAACAGTCAC CCTGTGGTAG GTTTGAAGAC 
CCTTGGGTCC TCCGACCGAG AAGTCAGAGT ATCATAAGTG CAGCTAGCAG TGGGGTATCT 
GCACCAGCTG CTGTATGTCC GGTCACACCA ACACACAGTG ACAGCAGCAG TCAGCGCTCA 
GACTATGCAG AGTCATGGGA CTTCTACATG GATTGCCCAT GTACACGGTC TGACCAGAGT 
CTTTCCTCAC AAATCACAAG GTCTGCAAGT GCTGAGGAGA ATCATGGAGT CATAGGTAAT 
GCATCCTACA ACAGTGAATT CCCATCATCC CCCTTCAGCA GTAGTCAGTC AAAATTGACC 
TCATCTCCTG ACAAAGTCCA TCGACTCACG TCACCATCAA GTGGCTACTC AAGTCAGTCT 
AACACTCCAA CTGCAGGCAC TCCAGTTACG TCTCTAATAA GAGCAAAGTC TCCTGCGGGG 
AGACCGAAAC CAAAAGTGCC AGAAAGAAAG TCCTCTCTAC GATCTTCTAT ATCATCCTCT 
TCTACATCCC TGTCCTCCAA CACCTCAGAT TCCATCAGGA ACATTCCTCC ACCTCCACCT 
TTACCTGACA TGACAACTGC ATTATGTGGG TCGCCCAGTT CTGCTTCTTT CTCTCCAACA 
GCGGCTAAAA TAGACCCAAT ACCCTCTTTT ACATCTCTAC AGGATGTATC ATCTGCCTTG 
GAAGATGGGC AAAATACACT TTCAGATACA TCTGCAACAC CTGAGGAATG TGATAGTCCT 
GATTTTCCCC CTCCCCATTC AAATGTTTTT GAATCTCTGT CAGGCAATGT AGATACCAAC 
AGCCTCCCAC CTCCTCCACT TCTGCTTTGC AAACCTTCAC TGTGCATTTC ATCTCTTCTT 
CCCCCTCCTC CTCCACCTCC TCCACTATTA CTTGAGACCA GTCTTCAACC AGCCACCATT 
TTGAAGACAG AGAGCAGTCT TACACCTACA ACAGCTGTAA AATGTGAAAA AGAAGTAAAC 
ATCAGTCACA CTGGAGAGCA AGAAAAGACT CAGAGACCAA TAATCACTGC CCAGGCCCTT 
CAGATGGTGC AGTTGCGACC TGTTAAGCTC AAGCAAATAG AAACTATTTT TACATCTGTC 
AGTTTTGATC ATTCTGATCA CCAAGCACAA AACCATTCTG ATACAAACTC AGGAAAGTCC 
CTGGAAATTG ATAATACAGG GCAAACAGTG ACCGTCAATT GTGTTACTCA GTCAGAAAAT 
AGTATGGAAA CATCTGAATG TGAATATTCG GTGAACAGAT TCATAGACGA TCTCCCTGAG 
GTATCAACTA GCACTCTACC CCCTGACCTA GATGAACAAT TAAAAAAAAC TGAGCAAAAT 
AATCCAGTGC ACCTCTCTTC CAAGTCCACA AATTCCTTAG CTTCTCCTCT GAGTTCTCCA 
ATGAAACAGA AGCCTCCTAT ATCACCCAAA AAACCCAATC TTTCCCTTAT TATACCACCC 
ATAATGCACT TGCCATTTTC CAACGGACTC AAGCTTCAGC AGGTTTATGA AGCACAAGAG 
ACACTAGAGT CAGAGCAAGT GAATGGTGCA TCCTCGACAA ACACAGTTAA TTTGCAAGCA 
ATTCCTTTAG ACCTTGAGGT AGAGGAAGAG AGTGACTCAG ACTTATCAAC CCCTGTGGTC 
AAGAGCAGAC TATCTTTCTG CAGCGAGTTA TCATCAAGCA GTTTCCAAGA CCTGCAACTT 
TCTGATTACA TTCTTAATGA GCATGACCTC GGCCTGAGCG ATAAAGACTT TGGGCTCTGT 
GATGATAAAA GCACATCAGA TGATGGTTCA AGCAGCAGCT CAGGATCCAT CAGCTTTAAA 
GAGGATGAAC ATGAGGAAAA CGGTGCTGTG TTTGACTCAA TCACTGAAAG CACATCTGAC 
ATTAATGCTA GCGGAGAAGC TGTGGAGGGG ATGGTGACGC CTATTCGCCC TCGTACTACA 
GAGGACCTCT TTGCTGCAAT TCATAGTCTA CGTAGACAGA ACATGAACAT CCAATTACAG 
GTCAAAAAGG AAGGTCCTGG GCCGTGGCGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_009293150.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291425.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.

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CloneID	ODa44940
Clone ID Related Accession (Same CDS sequence)	XM_009293150.2 , XM_021467438.1
Accession Version	XM_009293150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGAG AGAGGAGTTT TCTGAGGTAC AAGGGGGAGT GGGCAGAGGG TAAGAGTCAC 
CCTGTACCTC GAAACATTGC TGCCATGGGT CTTTCTTCTC TGACAGAGCA GCGTGGACGC 
TTCTTTCCCT GCCACAAAGA TGACACTGAT GACGATTATG ATGAGTGGGT TTATGCACCT 
CTCCATCGCA GAGCGGTGTC CAGTCTTGAT GAGGAGAGCA AATGGTCGGT GCACTACACA 
GCCCCCTGGC ACCACCAAGA GAACGTCTTC CTGCCTGGGT CCAGACCAGC ATGTGTTGAG 
GATCTTCACC GTCAGGCCAA AGTCAACCTG AAAACAGTCC TCAGAGAGTG TGACAAGCTC 
AGAAAAGATG GCTTTCGCAG TTCTCAGTAT TACTCCCAAA GCCCAGCATT CTCTGCAACC 
AGCCTTTGTG AAAGTGTACA TCTGGATGAA GAGAAGACAA AGAAAAAGAA GCGGTTTAAA 
GTCCTAGACT GCCTATCACA GTCTTGCCTC TCTCTCTGCT GTCATTTGAG ATCTTGGAAA 
ACAAAGGCTT CCGATTCACC TAATGAAGAG GATAAACTTG TGTACTCAAT GCCGCCACAA 
ACCCCTCTGC TGGAGCATGG CTCTGACATG GACATCCAGA GAAGCTGGAC CAGCTGTCTA 
CCTTTGCCCA CTCCCGAGGA GAAGATGAGA CTGCAGGCCC AATCGGTGGC TACTGATATA 
ATTCCTATCA ACATTACAGG TGAGAACTTT GACCGCCAGG CCAGCTTTCG CCGCTCCATG 
GCAAACACTG ATACTATTAT ACGAAGACCT AAGAGAGTTC AAAGAAGAAA GACAATAACA 
GGAGTGCCTG ACAACATCCA GACTGAACTA GCAAATAAAG AACAAGCTGA CTCCAGAATA 
CATTCGAGTA TGGCAGATCC ATACTCCACC CTCAGTCGAG TGGGAAGTGT AAACTCCACC 
CTGAAACGCT CAGACACCCG TGATTCCTGT TGTCAAACAG AAGAGGTGAA GATTGTTCCT 
CCCTCAGTAC GCAGAATCAG AGCACAAAGA GGCCATGGAA TAGCTGCCCA AATGGCCAGT 
GTCTCCTCCT CAAGCAGCAT TTCCACCATA AGTGACTGTA ATGGCATTAG CTACACACAG 
CTTAATGACA GTGATCAGAG CTTTCAAAGT TTACCAAGAC AAGGTTCTCG CATCCCGCAA 
CAACACAGTG AAACAAAGTA CAATAGCTCC CCCTACAGGG CGAATTCTGG TTCTACAAGC 
CCCTTGTCCT ACCATTTTAA CATGCAGCAT GACTCTTCAT CATCGCTGCA TATGCTGTCT 
GACTCGAGAA GCAAGCTTAC TGAAAATCTA AATTACGAAG GCTCTGATAA AAATAATACC 
TCAAGCTTGT TTAGTCTCAG TGGTGCTCTA CAGAACTCCA TTCCTTATGG ATACAAAAGG 
ACAGAGGACA ACCAAATGCA ATCACCTCAT TCGTCAGTCC AGTTGCTCAA TTCTGAGGAT 
GCCTCATTGT CGGGTGTCTC CTCGTGTTAT GAATCCACAG CGTCCCTCTG TACAGGTATT 
CATACAGAGA CTGAGTCCCA GTGCAGCACA CTTGATGGCA GAAACTGCTG CAGTCCTAAC 
TGTGTCCGCA GGGATTCCAA CTTCTCAGAG AGCAGCAACC AAAGCTGCAG CACACTAACC 
ACGGACCAAT GGACGTATGA AGTTTCTCCA AAAGACAATG TTACTTCAAG TTGCTCCTCA 
CCTGTCAGCC ATATATACCA CGCAGAGGAA CATTCTCCCA GCAAGACAGA TACAAGCTCA 
TTGTTCTCTG TTGACAATGA AGGTTACTAC ACCTCCATGC GCCTAGACTC TGGTTTGAAG 
TCACACAGTC ACGGCTGCAT CAATAAAGCA GGAGAACAAA AGCACAGTTT GTATGAGTGC 
AAAGACCACC ACAGTCAAAG TGATACTGCA AGTCTGCATA GCAACCGCTC CCTGACTCGT 
AGCATCTCTC TGCGAAAAGC CAGGAAGCCC CCACTCCCTC CAGAAAGAAC CGACTCTTTA 
AGGCGTAAAC CCCAGAGGAA GAGTCGCCAC AGTGGTTCAG TCCTTAATGA GCAGTTAATC 
TCTAGTCTCC AGCAGTCCTT ACAACTTAAC CTAAATACTC AAACTACATC TTGCTCTGAA 
CAGTCACCCT GTGGTAGGTT TGAAGACCCT TGGGTCCTCC GACCGAGAAG TCAGAGTATC 
ATAAGTGCAG CTAGCAGTGG GGTATCTGCA CCAGCTGCTG TATGTCCGGT CACACCAACA 
CACAGTGACA GCAGCAGTCA GCGCTCAGAC TATGCAGAGT CATGGGACTT CTACATGGAT 
TGCCCATGTA CACGGTCTGA CCAGAGTCTT TCCTCACAAA TCACAAGGTC TGCAAGTGCT 
GAGGAGAATC ATGGAGTCAT AGGTAATGCA TCCTACAACA GTGAATTCCC ATCATCCCCC 
TTCAGCAGTA GTCAGTCAAA ATTGACCTCA TCTCCTGACA AAGTCCATCG ACTCACGTCA 
CCATCAAGTG GCTACTCAAG TCAGTCTAAC ACTCCAACTG CAGGCACTCC AGTTACGTCT 
CTAATAAGAG CAAAGTCTCC TGCGGGGAGA CCGAAACCAA AAGTGCCAGA AAGAAAGTCC 
TCTCTACGAT CTTCTATATC ATCCTCTTCT ACATCCCTGT CCTCCAACAC CTCAGATTCC 
ATCAGGAACA TTCCTCCACC TCCACCTTTA CCTGACATGA CAACTGCATT ATGTGGGTCG 
CCCAGTTCTG CTTCTTTCTC TCCAACAGCG GCTAAAATAG ACCCAATACC CTCTTTTACA 
TCTCTACAGG ATGTATCATC TGCCTTGGAA GATGGGCAAA ATACACTTTC AGATACATCT 
GCAACACCTG AGGAATGTGA TAGTCCTGAT TTTCCCCCTC CCCATTCAAA TGTTTTTGAA 
TCTCTGTCAG GCAATGTAGA TACCAACAGC CTCCCACCTC CTCCACTTCT GCTTTGCAAA 
CCTTCACTGT GCATTTCATC TCTTCTTCCC CCTCCTCCTC CACCTCCTCC ACTATTACTT 
GAGACCAGTC TTCAACCAGC CACCATTTTG AAGACAGAGA GCAGTCTTAC ACCTACAACA 
GCTGTAAAAT GTGAAAAAGA AGTAAACATC AGTCACACTG GAGAGCAAGA AAAGACTCAG 
AGACCAATAA TCACTGCCCA GGCCCTTCAG ATGGTGCAGT TGCGACCTGT TAAGCTCAAG 
CAAATAGAAA CTATTTTTAC ATCTGTCAGT TTTGATCATT CTGATCACCA AGCACAAAAC 
CATTCTGATA CAAACTCAGG AAAGTCCCTG GAAATTGATA ATACAGGGCA AACAGTGACC 
GTCAATTGTG TTACTCAGTC AGAAAATAGT ATGGAAACAT CTGAATGTGA ATATTCGGTG 
AACAGATTCA TAGACGATCT CCCTGAGGTA TCAACTAGCA CTCTACCCCC TGACCTAGAT 
GAACAATTAA AAAAAACTGA GCAAAATAAT CCAGTGCACC TCTCTTCCAA GTCCACAAAT 
TCCTTAGCTT CTCCTCTGAG TTCTCCAATG AAACAGAAGC CTCCTATATC ACCCAAAAAA 
CCCAATCTTT CCCTTATTAT ACCACCCATA ATGCACTTGC CATTTTCCAA CGGACTCAAG 
CTTCAGCAGG TTTATGAAGC ACAAGAGACA CTAGAGTCAG AGCAAGTGAA TGGTGCATCC 
TCGACAAACA CAGTTAATTT GCAAGCAATT CCTTTAGACC TTGAGGTAGA GGAAGAGAGT 
GACTCAGACT TATCAACCCC TGTGGTCAAG AGCAGACTAT CTTTCTGCAG CGAGTTATCA 
TCAAGCAGTT TCCAAGACCT GCAACTTTCT GATTACATTC TTAATGAGCA TGACCTCGGC 
CTGAGCGATA AAGACTTTGG GCTCTGTGAT GATAAAAGCA CATCAGATGA TGGTTCAAGC 
AGCAGCTCAG GATCCATCAG CTTTAAAGAG GATGAACATG AGGAAAACGG TGCTGTGTTT 
GACTCAATCA CTGAAAGCAC ATCTGACATT AATGCTAGCG GAGAAGCTGT GGAGGGGATG 
GTGACGCCTA TTCGCCCTCG TACTACAGAG GACCTCTTTG CTGCAATTCA TAGGTCAAAA 
AGGAAGGTCC TGGGCCGTGG CGATTCTGAA GATGAACGCT GCCGTAACCT CCTTCCTTCC 
CCACCTGTCA CTCCAACTGG CTCCTGCCCC AGCTTGCCAC GTCAGACCAG CTCCATCCAG 
CGGAACCTAC GTCGCTCCAC CACAAGCAAT GACAGTTTTA AAGCTTTGCT ACTCAAGAAG 
GGCAGCCGCT CAGAGTCCAG CTTTCGCATG TCAGCCGCTG AGATTCTCAA ATGCACAGAC 
CCCCGTTTCC AAAGGGCTAA TTCAGAAAAT GCACTAGCTG ACGGACTGTC CTCTCCGACA 
AGCAGCAGGA GAGCACATGA AGAGTGGGCA CGGACGGAGG GAGCTTTGCC TCGCTTTACC 
ACTGGACAAA CTATCCCAAA ATATGGGCGC TCGCGCACAC CACCTTCCGC AGCCAGCAGC 
CGGTATAACA GCCGCAGCCG GATTACCAGT AGGCCAATGA CTGCCATCTG TGAACGAGAT 
GGAGAGGTGG CTGAATCTAC AGACTGCTAC TTAAGTAGCG AGAATCATTT TGTCTTGGCT 
TTATCCTCCA GCAGCATGGT CTGCGCTCAA GGCAGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291425.1
CDS	369..5090
Translation	MWGERSFLRYKGEWAEGKSHPVPRNIAAMGLSSLTEQRGRFFPCHKDDTDDDYDEWVYAPLHRRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_009293150.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293150.2

ORF Insert Sequence:

ATGTGGGGAG AGAGGAGTTT TCTGAGGTAC AAGGGGGAGT GGGCAGAGGG TAAGAGTCAC 
CCTGTACCTC GAAACATTGC TGCCATGGGT CTTTCTTCTC TGACAGAGCA GCGTGGACGC 
TTCTTTCCCT GCCACAAAGA TGACACTGAT GACGATTATG ATGAGTGGGT TTATGCACCT 
CTCCATCGCA GAGCGGTGTC CAGTCTTGAT GAGGAGAGCA AATGGTCGGT GCACTACACA 
GCCCCCTGGC ACCACCAAGA GAACGTCTTC CTGCCTGGGT CCAGACCAGC ATGTGTTGAG 
GATCTTCACC GTCAGGCCAA AGTCAACCTG AAAACAGTCC TCAGAGAGTG TGACAAGCTC 
AGAAAAGATG GCTTTCGCAG TTCTCAGTAT TACTCCCAAA GCCCAGCATT CTCTGCAACC 
AGCCTTTGTG AAAGTGTACA TCTGGATGAA GAGAAGACAA AGAAAAAGAA GCGGTTTAAA 
GTCCTAGACT GCCTATCACA GTCTTGCCTC TCTCTCTGCT GTCATTTGAG ATCTTGGAAA 
ACAAAGGCTT CCGATTCACC TAATGAAGAG GATAAACTTG TGTACTCAAT GCCGCCACAA 
ACCCCTCTGC TGGAGCATGG CTCTGACATG GACATCCAGA GAAGCTGGAC CAGCTGTCTA 
CCTTTGCCCA CTCCCGAGGA GAAGATGAGA CTGCAGGCCC AATCGGTGGC TACTGATATA 
ATTCCTATCA ACATTACAGG TGAGAACTTT GACCGCCAGG CCAGCTTTCG CCGCTCCATG 
GCAAACACTG ATACTATTAT ACGAAGACCT AAGAGAGTTC AAAGAAGAAA GACAATAACA 
GGAGTGCCTG ACAACATCCA GACTGAACTA GCAAATAAAG AACAAGCTGA CTCCAGAATA 
CATTCGAGTA TGGCAGATCC ATACTCCACC CTCAGTCGAG TGGGAAGTGT AAACTCCACC 
CTGAAACGCT CAGACACCCG TGATTCCTGT TGTCAAACAG AAGAGGTGAA GATTGTTCCT 
CCCTCAGTAC GCAGAATCAG AGCACAAAGA GGCCATGGAA TAGCTGCCCA AATGGCCAGT 
GTCTCCTCCT CAAGCAGCAT TTCCACCATA AGTGACTGTA ATGGCATTAG CTACACACAG 
CTTAATGACA GTGATCAGAG CTTTCAAAGT TTACCAAGAC AAGGTTCTCG CATCCCGCAA 
CAACACAGTG AAACAAAGTA CAATAGCTCC CCCTACAGGG CGAATTCTGG TTCTACAAGC 
CCCTTGTCCT ACCATTTTAA CATGCAGCAT GACTCTTCAT CATCGCTGCA TATGCTGTCT 
GACTCGAGAA GCAAGCTTAC TGAAAATCTA AATTACGAAG GCTCTGATAA AAATAATACC 
TCAAGCTTGT TTAGTCTCAG TGGTGCTCTA CAGAACTCCA TTCCTTATGG ATACAAAAGG 
ACAGAGGACA ACCAAATGCA ATCACCTCAT TCGTCAGTCC AGTTGCTCAA TTCTGAGGAT 
GCCTCATTGT CGGGTGTCTC CTCGTGTTAT GAATCCACAG CGTCCCTCTG TACAGGTATT 
CATACAGAGA CTGAGTCCCA GTGCAGCACA CTTGATGGCA GAAACTGCTG CAGTCCTAAC 
TGTGTCCGCA GGGATTCCAA CTTCTCAGAG AGCAGCAACC AAAGCTGCAG CACACTAACC 
ACGGACCAAT GGACGTATGA AGTTTCTCCA AAAGACAATG TTACTTCAAG TTGCTCCTCA 
CCTGTCAGCC ATATATACCA CGCAGAGGAA CATTCTCCCA GCAAGACAGA TACAAGCTCA 
TTGTTCTCTG TTGACAATGA AGGTTACTAC ACCTCCATGC GCCTAGACTC TGGTTTGAAG 
TCACACAGTC ACGGCTGCAT CAATAAAGCA GGAGAACAAA AGCACAGTTT GTATGAGTGC 
AAAGACCACC ACAGTCAAAG TGATACTGCA AGTCTGCATA GCAACCGCTC CCTGACTCGT 
AGCATCTCTC TGCGAAAAGC CAGGAAGCCC CCACTCCCTC CAGAAAGAAC CGACTCTTTA 
AGGCGTAAAC CCCAGAGGAA GAGTCGCCAC AGTGGTTCAG TCCTTAATGA GCAGTTAATC 
TCTAGTCTCC AGCAGTCCTT ACAACTTAAC CTAAATACTC AAACTACATC TTGCTCTGAA 
CAGTCACCCT GTGGTAGGTT TGAAGACCCT TGGGTCCTCC GACCGAGAAG TCAGAGTATC 
ATAAGTGCAG CTAGCAGTGG GGTATCTGCA CCAGCTGCTG TATGTCCGGT CACACCAACA 
CACAGTGACA GCAGCAGTCA GCGCTCAGAC TATGCAGAGT CATGGGACTT CTACATGGAT 
TGCCCATGTA CACGGTCTGA CCAGAGTCTT TCCTCACAAA TCACAAGGTC TGCAAGTGCT 
GAGGAGAATC ATGGAGTCAT AGGTAATGCA TCCTACAACA GTGAATTCCC ATCATCCCCC 
TTCAGCAGTA GTCAGTCAAA ATTGACCTCA TCTCCTGACA AAGTCCATCG ACTCACGTCA 
CCATCAAGTG GCTACTCAAG TCAGTCTAAC ACTCCAACTG CAGGCACTCC AGTTACGTCT 
CTAATAAGAG CAAAGTCTCC TGCGGGGAGA CCGAAACCAA AAGTGCCAGA AAGAAAGTCC 
TCTCTACGAT CTTCTATATC ATCCTCTTCT ACATCCCTGT CCTCCAACAC CTCAGATTCC 
ATCAGGAACA TTCCTCCACC TCCACCTTTA CCTGACATGA CAACTGCATT ATGTGGGTCG 
CCCAGTTCTG CTTCTTTCTC TCCAACAGCG GCTAAAATAG ACCCAATACC CTCTTTTACA 
TCTCTACAGG ATGTATCATC TGCCTTGGAA GATGGGCAAA ATACACTTTC AGATACATCT 
GCAACACCTG AGGAATGTGA TAGTCCTGAT TTTCCCCCTC CCCATTCAAA TGTTTTTGAA 
TCTCTGTCAG GCAATGTAGA TACCAACAGC CTCCCACCTC CTCCACTTCT GCTTTGCAAA 
CCTTCACTGT GCATTTCATC TCTTCTTCCC CCTCCTCCTC CACCTCCTCC ACTATTACTT 
GAGACCAGTC TTCAACCAGC CACCATTTTG AAGACAGAGA GCAGTCTTAC ACCTACAACA 
GCTGTAAAAT GTGAAAAAGA AGTAAACATC AGTCACACTG GAGAGCAAGA AAAGACTCAG 
AGACCAATAA TCACTGCCCA GGCCCTTCAG ATGGTGCAGT TGCGACCTGT TAAGCTCAAG 
CAAATAGAAA CTATTTTTAC ATCTGTCAGT TTTGATCATT CTGATCACCA AGCACAAAAC 
CATTCTGATA CAAACTCAGG AAAGTCCCTG GAAATTGATA ATACAGGGCA AACAGTGACC 
GTCAATTGTG TTACTCAGTC AGAAAATAGT ATGGAAACAT CTGAATGTGA ATATTCGGTG 
AACAGATTCA TAGACGATCT CCCTGAGGTA TCAACTAGCA CTCTACCCCC TGACCTAGAT 
GAACAATTAA AAAAAACTGA GCAAAATAAT CCAGTGCACC TCTCTTCCAA GTCCACAAAT 
TCCTTAGCTT CTCCTCTGAG TTCTCCAATG AAACAGAAGC CTCCTATATC ACCCAAAAAA 
CCCAATCTTT CCCTTATTAT ACCACCCATA ATGCACTTGC CATTTTCCAA CGGACTCAAG 
CTTCAGCAGG TTTATGAAGC ACAAGAGACA CTAGAGTCAG AGCAAGTGAA TGGTGCATCC 
TCGACAAACA CAGTTAATTT GCAAGCAATT CCTTTAGACC TTGAGGTAGA GGAAGAGAGT 
GACTCAGACT TATCAACCCC TGTGGTCAAG AGCAGACTAT CTTTCTGCAG CGAGTTATCA 
TCAAGCAGTT TCCAAGACCT GCAACTTTCT GATTACATTC TTAATGAGCA TGACCTCGGC 
CTGAGCGATA AAGACTTTGG GCTCTGTGAT GATAAAAGCA CATCAGATGA TGGTTCAAGC 
AGCAGCTCAG GATCCATCAG CTTTAAAGAG GATGAACATG AGGAAAACGG TGCTGTGTTT 
GACTCAATCA CTGAAAGCAC ATCTGACATT AATGCTAGCG GAGAAGCTGT GGAGGGGATG 
GTGACGCCTA TTCGCCCTCG TACTACAGAG GACCTCTTTG CTGCAATTCA TAGGTCAAAA 
AGGAAGGTCC TGGGCCGTGG CGATTCTGAA GATGAACGCT GCCGTAACCT CCTTCCTTCC 
CCACCTGTCA CTCCAACTGG CTCCTGCCCC AGCTTGCCAC GTCAGACCAG CTCCATCCAG 
CGGAACCTAC GTCGCTCCAC CACAAGCAAT GACAGTTTTA AAGCTTTGCT ACTCAAGAAG 
GGCAGCCGCT CAGAGTCCAG CTTTCGCATG TCAGCCGCTG AGATTCTCAA ATGCACAGAC 
CCCCGTTTCC AAAGGGCTAA TTCAGAAAAT GCACTAGCTG ACGGACTGTC CTCTCCGACA 
AGCAGCAGGA GAGCACATGA AGAGTGGGCA CGGACGGAGG GAGCTTTGCC TCGCTTTACC 
ACTGGACAAA CTATCCCAAA ATATGGGCGC TCGCGCACAC CACCTTCCGC AGCCAGCAGC 
CGGTATAACA GCCGCAGCCG GATTACCAGT AGGCCAATGA CTGCCATCTG TGAACGAGAT 
GGAGAGGTGG CTGAATCTAC AGACTGCTAC TTAAGTAGCG AGAATCATTT TGTCTTGGCT 
TTATCCTCCA GCAGCATGGT CTGCGCTCAA GGCAGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1a ( XM_021467438.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323113.1 Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.

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CloneID	ODa44940
Clone ID Related Accession (Same CDS sequence)	XM_009293150.2 , XM_021467438.1
Accession Version	XM_021467438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGAG AGAGGAGTTT TCTGAGGTAC AAGGGGGAGT GGGCAGAGGG TAAGAGTCAC 
CCTGTACCTC GAAACATTGC TGCCATGGGT CTTTCTTCTC TGACAGAGCA GCGTGGACGC 
TTCTTTCCCT GCCACAAAGA TGACACTGAT GACGATTATG ATGAGTGGGT TTATGCACCT 
CTCCATCGCA GAGCGGTGTC CAGTCTTGAT GAGGAGAGCA AATGGTCGGT GCACTACACA 
GCCCCCTGGC ACCACCAAGA GAACGTCTTC CTGCCTGGGT CCAGACCAGC ATGTGTTGAG 
GATCTTCACC GTCAGGCCAA AGTCAACCTG AAAACAGTCC TCAGAGAGTG TGACAAGCTC 
AGAAAAGATG GCTTTCGCAG TTCTCAGTAT TACTCCCAAA GCCCAGCATT CTCTGCAACC 
AGCCTTTGTG AAAGTGTACA TCTGGATGAA GAGAAGACAA AGAAAAAGAA GCGGTTTAAA 
GTCCTAGACT GCCTATCACA GTCTTGCCTC TCTCTCTGCT GTCATTTGAG ATCTTGGAAA 
ACAAAGGCTT CCGATTCACC TAATGAAGAG GATAAACTTG TGTACTCAAT GCCGCCACAA 
ACCCCTCTGC TGGAGCATGG CTCTGACATG GACATCCAGA GAAGCTGGAC CAGCTGTCTA 
CCTTTGCCCA CTCCCGAGGA GAAGATGAGA CTGCAGGCCC AATCGGTGGC TACTGATATA 
ATTCCTATCA ACATTACAGG TGAGAACTTT GACCGCCAGG CCAGCTTTCG CCGCTCCATG 
GCAAACACTG ATACTATTAT ACGAAGACCT AAGAGAGTTC AAAGAAGAAA GACAATAACA 
GGAGTGCCTG ACAACATCCA GACTGAACTA GCAAATAAAG AACAAGCTGA CTCCAGAATA 
CATTCGAGTA TGGCAGATCC ATACTCCACC CTCAGTCGAG TGGGAAGTGT AAACTCCACC 
CTGAAACGCT CAGACACCCG TGATTCCTGT TGTCAAACAG AAGAGGTGAA GATTGTTCCT 
CCCTCAGTAC GCAGAATCAG AGCACAAAGA GGCCATGGAA TAGCTGCCCA AATGGCCAGT 
GTCTCCTCCT CAAGCAGCAT TTCCACCATA AGTGACTGTA ATGGCATTAG CTACACACAG 
CTTAATGACA GTGATCAGAG CTTTCAAAGT TTACCAAGAC AAGGTTCTCG CATCCCGCAA 
CAACACAGTG AAACAAAGTA CAATAGCTCC CCCTACAGGG CGAATTCTGG TTCTACAAGC 
CCCTTGTCCT ACCATTTTAA CATGCAGCAT GACTCTTCAT CATCGCTGCA TATGCTGTCT 
GACTCGAGAA GCAAGCTTAC TGAAAATCTA AATTACGAAG GCTCTGATAA AAATAATACC 
TCAAGCTTGT TTAGTCTCAG TGGTGCTCTA CAGAACTCCA TTCCTTATGG ATACAAAAGG 
ACAGAGGACA ACCAAATGCA ATCACCTCAT TCGTCAGTCC AGTTGCTCAA TTCTGAGGAT 
GCCTCATTGT CGGGTGTCTC CTCGTGTTAT GAATCCACAG CGTCCCTCTG TACAGGTATT 
CATACAGAGA CTGAGTCCCA GTGCAGCACA CTTGATGGCA GAAACTGCTG CAGTCCTAAC 
TGTGTCCGCA GGGATTCCAA CTTCTCAGAG AGCAGCAACC AAAGCTGCAG CACACTAACC 
ACGGACCAAT GGACGTATGA AGTTTCTCCA AAAGACAATG TTACTTCAAG TTGCTCCTCA 
CCTGTCAGCC ATATATACCA CGCAGAGGAA CATTCTCCCA GCAAGACAGA TACAAGCTCA 
TTGTTCTCTG TTGACAATGA AGGTTACTAC ACCTCCATGC GCCTAGACTC TGGTTTGAAG 
TCACACAGTC ACGGCTGCAT CAATAAAGCA GGAGAACAAA AGCACAGTTT GTATGAGTGC 
AAAGACCACC ACAGTCAAAG TGATACTGCA AGTCTGCATA GCAACCGCTC CCTGACTCGT 
AGCATCTCTC TGCGAAAAGC CAGGAAGCCC CCACTCCCTC CAGAAAGAAC CGACTCTTTA 
AGGCGTAAAC CCCAGAGGAA GAGTCGCCAC AGTGGTTCAG TCCTTAATGA GCAGTTAATC 
TCTAGTCTCC AGCAGTCCTT ACAACTTAAC CTAAATACTC AAACTACATC TTGCTCTGAA 
CAGTCACCCT GTGGTAGGTT TGAAGACCCT TGGGTCCTCC GACCGAGAAG TCAGAGTATC 
ATAAGTGCAG CTAGCAGTGG GGTATCTGCA CCAGCTGCTG TATGTCCGGT CACACCAACA 
CACAGTGACA GCAGCAGTCA GCGCTCAGAC TATGCAGAGT CATGGGACTT CTACATGGAT 
TGCCCATGTA CACGGTCTGA CCAGAGTCTT TCCTCACAAA TCACAAGGTC TGCAAGTGCT 
GAGGAGAATC ATGGAGTCAT AGGTAATGCA TCCTACAACA GTGAATTCCC ATCATCCCCC 
TTCAGCAGTA GTCAGTCAAA ATTGACCTCA TCTCCTGACA AAGTCCATCG ACTCACGTCA 
CCATCAAGTG GCTACTCAAG TCAGTCTAAC ACTCCAACTG CAGGCACTCC AGTTACGTCT 
CTAATAAGAG CAAAGTCTCC TGCGGGGAGA CCGAAACCAA AAGTGCCAGA AAGAAAGTCC 
TCTCTACGAT CTTCTATATC ATCCTCTTCT ACATCCCTGT CCTCCAACAC CTCAGATTCC 
ATCAGGAACA TTCCTCCACC TCCACCTTTA CCTGACATGA CAACTGCATT ATGTGGGTCG 
CCCAGTTCTG CTTCTTTCTC TCCAACAGCG GCTAAAATAG ACCCAATACC CTCTTTTACA 
TCTCTACAGG ATGTATCATC TGCCTTGGAA GATGGGCAAA ATACACTTTC AGATACATCT 
GCAACACCTG AGGAATGTGA TAGTCCTGAT TTTCCCCCTC CCCATTCAAA TGTTTTTGAA 
TCTCTGTCAG GCAATGTAGA TACCAACAGC CTCCCACCTC CTCCACTTCT GCTTTGCAAA 
CCTTCACTGT GCATTTCATC TCTTCTTCCC CCTCCTCCTC CACCTCCTCC ACTATTACTT 
GAGACCAGTC TTCAACCAGC CACCATTTTG AAGACAGAGA GCAGTCTTAC ACCTACAACA 
GCTGTAAAAT GTGAAAAAGA AGTAAACATC AGTCACACTG GAGAGCAAGA AAAGACTCAG 
AGACCAATAA TCACTGCCCA GGCCCTTCAG ATGGTGCAGT TGCGACCTGT TAAGCTCAAG 
CAAATAGAAA CTATTTTTAC ATCTGTCAGT TTTGATCATT CTGATCACCA AGCACAAAAC 
CATTCTGATA CAAACTCAGG AAAGTCCCTG GAAATTGATA ATACAGGGCA AACAGTGACC 
GTCAATTGTG TTACTCAGTC AGAAAATAGT ATGGAAACAT CTGAATGTGA ATATTCGGTG 
AACAGATTCA TAGACGATCT CCCTGAGGTA TCAACTAGCA CTCTACCCCC TGACCTAGAT 
GAACAATTAA AAAAAACTGA GCAAAATAAT CCAGTGCACC TCTCTTCCAA GTCCACAAAT 
TCCTTAGCTT CTCCTCTGAG TTCTCCAATG AAACAGAAGC CTCCTATATC ACCCAAAAAA 
CCCAATCTTT CCCTTATTAT ACCACCCATA ATGCACTTGC CATTTTCCAA CGGACTCAAG 
CTTCAGCAGG TTTATGAAGC ACAAGAGACA CTAGAGTCAG AGCAAGTGAA TGGTGCATCC 
TCGACAAACA CAGTTAATTT GCAAGCAATT CCTTTAGACC TTGAGGTAGA GGAAGAGAGT 
GACTCAGACT TATCAACCCC TGTGGTCAAG AGCAGACTAT CTTTCTGCAG CGAGTTATCA 
TCAAGCAGTT TCCAAGACCT GCAACTTTCT GATTACATTC TTAATGAGCA TGACCTCGGC 
CTGAGCGATA AAGACTTTGG GCTCTGTGAT GATAAAAGCA CATCAGATGA TGGTTCAAGC 
AGCAGCTCAG GATCCATCAG CTTTAAAGAG GATGAACATG AGGAAAACGG TGCTGTGTTT 
GACTCAATCA CTGAAAGCAC ATCTGACATT AATGCTAGCG GAGAAGCTGT GGAGGGGATG 
GTGACGCCTA TTCGCCCTCG TACTACAGAG GACCTCTTTG CTGCAATTCA TAGGTCAAAA 
AGGAAGGTCC TGGGCCGTGG CGATTCTGAA GATGAACGCT GCCGTAACCT CCTTCCTTCC 
CCACCTGTCA CTCCAACTGG CTCCTGCCCC AGCTTGCCAC GTCAGACCAG CTCCATCCAG 
CGGAACCTAC GTCGCTCCAC CACAAGCAAT GACAGTTTTA AAGCTTTGCT ACTCAAGAAG 
GGCAGCCGCT CAGAGTCCAG CTTTCGCATG TCAGCCGCTG AGATTCTCAA ATGCACAGAC 
CCCCGTTTCC AAAGGGCTAA TTCAGAAAAT GCACTAGCTG ACGGACTGTC CTCTCCGACA 
AGCAGCAGGA GAGCACATGA AGAGTGGGCA CGGACGGAGG GAGCTTTGCC TCGCTTTACC 
ACTGGACAAA CTATCCCAAA ATATGGGCGC TCGCGCACAC CACCTTCCGC AGCCAGCAGC 
CGGTATAACA GCCGCAGCCG GATTACCAGT AGGCCAATGA CTGCCATCTG TGAACGAGAT 
GGAGAGGTGG CTGAATCTAC AGACTGCTAC TTAAGTAGCG AGAATCATTT TGTCTTGGCT 
TTATCCTCCA GCAGCATGGT CTGCGCTCAA GGCAGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323113.1
CDS	119..4840
Translation	MWGERSFLRYKGEWAEGKSHPVPRNIAAMGLSSLTEQRGRFFPCHKDDTDDDYDEWVYAPLHRRAVSSLDEESKWSVHYTAPWHHQENVFLPGSRPACVEDLHRQAKVNLKTVLRECDKLRKDGFRSSQYYSQSPAFSATSLCESVHLDEEKTKKKKRFKVLDCLSQSCLSLCCHLRSWKTKASDSPNEEDKLVYSMPPQTPLLEHGSDMDIQRSWTSCLPLPTPEEKMRLQAQSVATDIIPINITGENFDRQASFRRSMANTDTIIRRPKRVQRRKTITGVPDNIQTELANKEQADSRIHSSMADPYSTLSRVGSVNSTLKRSDTRDSCCQTEEVKIVPPSVRRIRAQRGHGIAAQMASVSSSSSISTISDCNGISYTQLNDSDQSFQSLPRQGSRIPQQHSETKYNSSPYRANSGSTSPLSYHFNMQHDSSSSLHMLSDSRSKLTENLNYEGSDKNNTSSLFSLSGALQNSIPYGYKRTEDNQMQSPHSSVQLLNSEDASLSGVSSCYESTASLCTGIHTETESQCSTLDGRNCCSPNCVRRDSNFSESSNQSCSTLTTDQWTYEVSPKDNVTSSCSSPVSHIYHAEEHSPSKTDTSSLFSVDNEGYYTSMRLDSGLKSHSHGCINKAGEQKHSLYECKDHHSQSDTASLHSNRSLTRSISLRKARKPPLPPERTDSLRRKPQRKSRHSGSVLNEQLISSLQQSLQLNLNTQTTSCSEQSPCGRFEDPWVLRPRSQSIISAASSGVSAPAAVCPVTPTHSDSSSQRSDYAESWDFYMDCPCTRSDQSLSSQITRSASAEENHGVIGNASYNSEFPSSPFSSSQSKLTSSPDKVHRLTSPSSGYSSQSNTPTAGTPVTSLIRAKSPAGRPKPKVPERKSSLRSSISSSSTSLSSNTSDSIRNIPPPPPLPDMTTALCGSPSSASFSPTAAKIDPIPSFTSLQDVSSALEDGQNTLSDTSATPEECDSPDFPPPHSNVFESLSGNVDTNSLPPPPLLLCKPSLCISSLLPPPPPPPPLLLETSLQPATILKTESSLTPTTAVKCEKEVNISHTGEQEKTQRPIITAQALQMVQLRPVKLKQIETIFTSVSFDHSDHQAQNHSDTNSGKSLEIDNTGQTVTVNCVTQSENSMETSECEYSVNRFIDDLPEVSTSTLPPDLDEQLKKTEQNNPVHLSSKSTNSLASPLSSPMKQKPPISPKKPNLSLIIPPIMHLPFSNGLKLQQVYEAQETLESEQVNGASSTNTVNLQAIPLDLEVEEESDSDLSTPVVKSRLSFCSELSSSSFQDLQLSDYILNEHDLGLSDKDFGLCDDKSTSDDGSSSSSGSISFKEDEHEENGAVFDSITESTSDINASGEAVEGMVTPIRPRTTEDLFAAIHRSKRKVLGRGDSEDERCRNLLPSPPVTPTGSCPSLPRQTSSIQRNLRRSTTSNDSFKALLLKKGSRSESSFRMSAAEILKCTDPRFQRANSENALADGLSSPTSSRRAHEEWARTEGALPRFTTGQTIPKYGRSRTPPSAASSRYNSRSRITSRPMTAICERDGEVAESTDCYLSSENHFVLALSSSSMVCAQGST

Target ORF information:

RefSeq Version	XM_021467438.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1a (nhsl1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467438.1

ORF Insert Sequence:

ATGTGGGGAG AGAGGAGTTT TCTGAGGTAC AAGGGGGAGT GGGCAGAGGG TAAGAGTCAC 
CCTGTACCTC GAAACATTGC TGCCATGGGT CTTTCTTCTC TGACAGAGCA GCGTGGACGC 
TTCTTTCCCT GCCACAAAGA TGACACTGAT GACGATTATG ATGAGTGGGT TTATGCACCT 
CTCCATCGCA GAGCGGTGTC CAGTCTTGAT GAGGAGAGCA AATGGTCGGT GCACTACACA 
GCCCCCTGGC ACCACCAAGA GAACGTCTTC CTGCCTGGGT CCAGACCAGC ATGTGTTGAG 
GATCTTCACC GTCAGGCCAA AGTCAACCTG AAAACAGTCC TCAGAGAGTG TGACAAGCTC 
AGAAAAGATG GCTTTCGCAG TTCTCAGTAT TACTCCCAAA GCCCAGCATT CTCTGCAACC 
AGCCTTTGTG AAAGTGTACA TCTGGATGAA GAGAAGACAA AGAAAAAGAA GCGGTTTAAA 
GTCCTAGACT GCCTATCACA GTCTTGCCTC TCTCTCTGCT GTCATTTGAG ATCTTGGAAA 
ACAAAGGCTT CCGATTCACC TAATGAAGAG GATAAACTTG TGTACTCAAT GCCGCCACAA 
ACCCCTCTGC TGGAGCATGG CTCTGACATG GACATCCAGA GAAGCTGGAC CAGCTGTCTA 
CCTTTGCCCA CTCCCGAGGA GAAGATGAGA CTGCAGGCCC AATCGGTGGC TACTGATATA 
ATTCCTATCA ACATTACAGG TGAGAACTTT GACCGCCAGG CCAGCTTTCG CCGCTCCATG 
GCAAACACTG ATACTATTAT ACGAAGACCT AAGAGAGTTC AAAGAAGAAA GACAATAACA 
GGAGTGCCTG ACAACATCCA GACTGAACTA GCAAATAAAG AACAAGCTGA CTCCAGAATA 
CATTCGAGTA TGGCAGATCC ATACTCCACC CTCAGTCGAG TGGGAAGTGT AAACTCCACC 
CTGAAACGCT CAGACACCCG TGATTCCTGT TGTCAAACAG AAGAGGTGAA GATTGTTCCT 
CCCTCAGTAC GCAGAATCAG AGCACAAAGA GGCCATGGAA TAGCTGCCCA AATGGCCAGT 
GTCTCCTCCT CAAGCAGCAT TTCCACCATA AGTGACTGTA ATGGCATTAG CTACACACAG 
CTTAATGACA GTGATCAGAG CTTTCAAAGT TTACCAAGAC AAGGTTCTCG CATCCCGCAA 
CAACACAGTG AAACAAAGTA CAATAGCTCC CCCTACAGGG CGAATTCTGG TTCTACAAGC 
CCCTTGTCCT ACCATTTTAA CATGCAGCAT GACTCTTCAT CATCGCTGCA TATGCTGTCT 
GACTCGAGAA GCAAGCTTAC TGAAAATCTA AATTACGAAG GCTCTGATAA AAATAATACC 
TCAAGCTTGT TTAGTCTCAG TGGTGCTCTA CAGAACTCCA TTCCTTATGG ATACAAAAGG 
ACAGAGGACA ACCAAATGCA ATCACCTCAT TCGTCAGTCC AGTTGCTCAA TTCTGAGGAT 
GCCTCATTGT CGGGTGTCTC CTCGTGTTAT GAATCCACAG CGTCCCTCTG TACAGGTATT 
CATACAGAGA CTGAGTCCCA GTGCAGCACA CTTGATGGCA GAAACTGCTG CAGTCCTAAC 
TGTGTCCGCA GGGATTCCAA CTTCTCAGAG AGCAGCAACC AAAGCTGCAG CACACTAACC 
ACGGACCAAT GGACGTATGA AGTTTCTCCA AAAGACAATG TTACTTCAAG TTGCTCCTCA 
CCTGTCAGCC ATATATACCA CGCAGAGGAA CATTCTCCCA GCAAGACAGA TACAAGCTCA 
TTGTTCTCTG TTGACAATGA AGGTTACTAC ACCTCCATGC GCCTAGACTC TGGTTTGAAG 
TCACACAGTC ACGGCTGCAT CAATAAAGCA GGAGAACAAA AGCACAGTTT GTATGAGTGC 
AAAGACCACC ACAGTCAAAG TGATACTGCA AGTCTGCATA GCAACCGCTC CCTGACTCGT 
AGCATCTCTC TGCGAAAAGC CAGGAAGCCC CCACTCCCTC CAGAAAGAAC CGACTCTTTA 
AGGCGTAAAC CCCAGAGGAA GAGTCGCCAC AGTGGTTCAG TCCTTAATGA GCAGTTAATC 
TCTAGTCTCC AGCAGTCCTT ACAACTTAAC CTAAATACTC AAACTACATC TTGCTCTGAA 
CAGTCACCCT GTGGTAGGTT TGAAGACCCT TGGGTCCTCC GACCGAGAAG TCAGAGTATC 
ATAAGTGCAG CTAGCAGTGG GGTATCTGCA CCAGCTGCTG TATGTCCGGT CACACCAACA 
CACAGTGACA GCAGCAGTCA GCGCTCAGAC TATGCAGAGT CATGGGACTT CTACATGGAT 
TGCCCATGTA CACGGTCTGA CCAGAGTCTT TCCTCACAAA TCACAAGGTC TGCAAGTGCT 
GAGGAGAATC ATGGAGTCAT AGGTAATGCA TCCTACAACA GTGAATTCCC ATCATCCCCC 
TTCAGCAGTA GTCAGTCAAA ATTGACCTCA TCTCCTGACA AAGTCCATCG ACTCACGTCA 
CCATCAAGTG GCTACTCAAG TCAGTCTAAC ACTCCAACTG CAGGCACTCC AGTTACGTCT 
CTAATAAGAG CAAAGTCTCC TGCGGGGAGA CCGAAACCAA AAGTGCCAGA AAGAAAGTCC 
TCTCTACGAT CTTCTATATC ATCCTCTTCT ACATCCCTGT CCTCCAACAC CTCAGATTCC 
ATCAGGAACA TTCCTCCACC TCCACCTTTA CCTGACATGA CAACTGCATT ATGTGGGTCG 
CCCAGTTCTG CTTCTTTCTC TCCAACAGCG GCTAAAATAG ACCCAATACC CTCTTTTACA 
TCTCTACAGG ATGTATCATC TGCCTTGGAA GATGGGCAAA ATACACTTTC AGATACATCT 
GCAACACCTG AGGAATGTGA TAGTCCTGAT TTTCCCCCTC CCCATTCAAA TGTTTTTGAA 
TCTCTGTCAG GCAATGTAGA TACCAACAGC CTCCCACCTC CTCCACTTCT GCTTTGCAAA 
CCTTCACTGT GCATTTCATC TCTTCTTCCC CCTCCTCCTC CACCTCCTCC ACTATTACTT 
GAGACCAGTC TTCAACCAGC CACCATTTTG AAGACAGAGA GCAGTCTTAC ACCTACAACA 
GCTGTAAAAT GTGAAAAAGA AGTAAACATC AGTCACACTG GAGAGCAAGA AAAGACTCAG 
AGACCAATAA TCACTGCCCA GGCCCTTCAG ATGGTGCAGT TGCGACCTGT TAAGCTCAAG 
CAAATAGAAA CTATTTTTAC ATCTGTCAGT TTTGATCATT CTGATCACCA AGCACAAAAC 
CATTCTGATA CAAACTCAGG AAAGTCCCTG GAAATTGATA ATACAGGGCA AACAGTGACC 
GTCAATTGTG TTACTCAGTC AGAAAATAGT ATGGAAACAT CTGAATGTGA ATATTCGGTG 
AACAGATTCA TAGACGATCT CCCTGAGGTA TCAACTAGCA CTCTACCCCC TGACCTAGAT 
GAACAATTAA AAAAAACTGA GCAAAATAAT CCAGTGCACC TCTCTTCCAA GTCCACAAAT 
TCCTTAGCTT CTCCTCTGAG TTCTCCAATG AAACAGAAGC CTCCTATATC ACCCAAAAAA 
CCCAATCTTT CCCTTATTAT ACCACCCATA ATGCACTTGC CATTTTCCAA CGGACTCAAG 
CTTCAGCAGG TTTATGAAGC ACAAGAGACA CTAGAGTCAG AGCAAGTGAA TGGTGCATCC 
TCGACAAACA CAGTTAATTT GCAAGCAATT CCTTTAGACC TTGAGGTAGA GGAAGAGAGT 
GACTCAGACT TATCAACCCC TGTGGTCAAG AGCAGACTAT CTTTCTGCAG CGAGTTATCA 
TCAAGCAGTT TCCAAGACCT GCAACTTTCT GATTACATTC TTAATGAGCA TGACCTCGGC 
CTGAGCGATA AAGACTTTGG GCTCTGTGAT GATAAAAGCA CATCAGATGA TGGTTCAAGC 
AGCAGCTCAG GATCCATCAG CTTTAAAGAG GATGAACATG AGGAAAACGG TGCTGTGTTT 
GACTCAATCA CTGAAAGCAC ATCTGACATT AATGCTAGCG GAGAAGCTGT GGAGGGGATG 
GTGACGCCTA TTCGCCCTCG TACTACAGAG GACCTCTTTG CTGCAATTCA TAGGTCAAAA 
AGGAAGGTCC TGGGCCGTGG CGATTCTGAA GATGAACGCT GCCGTAACCT CCTTCCTTCC 
CCACCTGTCA CTCCAACTGG CTCCTGCCCC AGCTTGCCAC GTCAGACCAG CTCCATCCAG 
CGGAACCTAC GTCGCTCCAC CACAAGCAAT GACAGTTTTA AAGCTTTGCT ACTCAAGAAG 
GGCAGCCGCT CAGAGTCCAG CTTTCGCATG TCAGCCGCTG AGATTCTCAA ATGCACAGAC 
CCCCGTTTCC AAAGGGCTAA TTCAGAAAAT GCACTAGCTG ACGGACTGTC CTCTCCGACA 
AGCAGCAGGA GAGCACATGA AGAGTGGGCA CGGACGGAGG GAGCTTTGCC TCGCTTTACC 
ACTGGACAAA CTATCCCAAA ATATGGGCGC TCGCGCACAC CACCTTCCGC AGCCAGCAGC 
CGGTATAACA GCCGCAGCCG GATTACCAGT AGGCCAATGA CTGCCATCTG TGAACGAGAT 
GGAGAGGTGG CTGAATCTAC AGACTGCTAC TTAAGTAGCG AGAATCATTT TGTCTTGGCT 
TTATCCTCCA GCAGCATGGT CTGCGCTCAA GGCAGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nhsl1a
Entrez Gene ID	566695
Full Name	NHS-like 1a
Synonyms	im:7139028
General protein information	Preferred Names NHS-like 1a Names NHS-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nhsl1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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