si:dkey-42p8.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:dkey-42p8.3
Entrez Gene ID	564019
Full Name	transducin like enhancer of split 2b
Synonyms	si:dkey-42p8.3,tle1
General protein information	Preferred Names transducin like enhancer of split 2b Names transducin-like enhancer protein 4 transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021473487.1	XP_021329162.1	transducin-like enhancer protein 4 isoform X3
XM_021473488.1	XP_021329163.1	transducin-like enhancer protein 4 isoform X4
XM_687379.6	XP_692471.4	transducin-like enhancer protein 4 isoform X1
XM_005170536.3	XP_005170593.1	transducin-like enhancer protein 4 isoform X2
XM_687379.7	XP_692471.4	transducin-like enhancer protein 4 isoform X1
XM_005170536.4	XP_005170593.1	transducin-like enhancer protein 4 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following si:dkey-42p8.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-42p8.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26974	XM_005170536.4 Latest version!	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa26974	XM_021473488.1 Latest version!	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa26975	XM_021473487.1 Latest version!	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa26975	XM_687379.7 Latest version!	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa26975	XM_687379.6	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa26974	XM_005170536.3	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:dkey-42p8.3 ( XM_021473488.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329163.1 Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa26974
Clone ID Related Accession (Same CDS sequence)	XM_021473488.1 , XM_005170536.3 , XM_005170536.4
Accession Version	XM_021473488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transducin-like enhancer protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395105.1) and transcript sequence (GFIL01022447.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 3521 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCTCCA GCACCTGTCT CACCACACTC CAGGTATTCC TCTCACTCCT 
CATCCTTCAG GTCTGTCTCT GGGTGGGACG TCCAGTCTGT TGGCCCTCAC TGGGGCCCTG 
GGAGTCACGG CACACCTGGC CAACAAGGAC GAACGCAACC ACCTAGACCC AGAGCACCTA 
CGAGAGGGTG CGCCCAGCAG GAGTAAATCA GTGTCGTCGA CTGACAGTCA GGCGGTGGAG 
GATCGTCAGG GTCCGTCAGG GGTTTATTCC TCTCAGGCCG GTGGTGATGC CAAACGGAGA 
CGCTGTGAGG AAAAAGACAT TCTGCAGTCC CACTACGACA GTGATGGAGA CAAGAGCGAG 
GACAATCTTG TAGTAGACGT CTCGAATGAG GAAGCTTCCT CTCCGGTCGG CAGTGCTCCT 
CTCTCTCCTC ATGGAAATGG ACTGGATCGA GCTCTACCAC TGAGGAAAGA GCTCCCAGGA 
TCCCCCAGTC CCCCCACAGC CTCCCCGCCA CACAGCCCAA CCCCTGGCAA AAACAAGGAC 
ACGGCACAAA CAGAAAAATC TCAGTCGCCT TCCTCAAAAT CAGGCAATTC CTCTCCCTCT 
CACCATGACC CTCTCACCCC TGGTCCACCC GGCCCACCGG GACCCAGCAC GTCCTGTTTA 
CGCCTGGTCA ACAAACCTGC CTCCTCGGCA GACCCTCTGG CTCTGCGGAG CCCCTTAACC 
ATGACGCCCG GTTCTTACCC AGCTCACTTT GGGGTGGTGT CTCACGCTGG GCTGAATGGA 
GAGCTGGCCA GTCCGGGCGG ATTCGGAGGC TCACTCACCC TCTCGCCCCA AATCAGTGCT 
GCAGCCAGTG CCTACACACG CAGCCCTATG GTAGCGTATG AATCCCGTTC ACATCTCAGG 
CCCTCAGGGC TGTCCTCCTC ACTTCCTGGC TCTACTAGTG GCAAACCTGC TTACTCGTTC 
CATGTGAGCG CAGATGGTCA AATGCAGCCG GTGCCCTTTC CTCCTGATGC CCTCCTCGGC 
CCTGGCATCC CGCGTCATGC CCGTCAGATC CACACTCTGA GTCATGGAGA GGTGGTGTGC 
GCCGTCACCA TCAGCACTTC CACACGCCAC GTCTACACCG GTGGCAAAGG TTGTGTCAAA 
GTATGGGACA TCAGCCAACC AGGCAGCAAG AGCCCGATGG CCCAACTGGA CTGTTTGAAT 
CGAGACAACT ACATCCGCTC ATGCAAGATT CTCCCAGATG GCCGGACCTT AATAGTTGGG 
GGCGAGGCCA GCACACTGTC CATCTGGGAC TTGGCCACAC CCACTCCACG CATCAAGGCA 
GAATTGACGT CTTCGGCTCC GGCCTGCTAC GCTCTGGCCA TCAGTCCTGA CAACAAGGTC 
TGCTTCTCCT GCTGCAGTGA CGGAAACATC GTAGTCTGGG ACCTGCACAA CCAGACACTC 
GTCAGGCAGT TCCAAGGCCA CACAGATGGA GCCAGTTGTA TTGATATTTC TAATGATGGG 
ACCAAATTGT GGACGGGAGG TCTGGACAAC ACTGTACGCT GCTGGGACCT GAGAGAAGGG 
CGACAGCTGC AGCAGCATGA CTTCACATCA CAGATCTTTT CTCTGGGTTA CTGTCCTACG 
GGTGAATGGC TTGCAGTGGG GATGGAGAGC AGTAATGTGG AAGTGCTGCA CGTCTCCAAA 
CCAGACAAAT ACCAGCTACA CCTTCATGAA AGCTGCGTTC TCTCGCTCAA GTTTGCCTAC 
TGCGGGAAGT GGTTTGTAAG CACAGGAAAG GACAATCTTC TCAATGCATG GAGGACGCCT 
TACGGAGCCA GTATTTTCCA GTCAAAGGAG ACCTCATCTG TCCTGAGCTG TGACGTGTCT 
CCAGATGACA AGTATATTGT AACTGGATCT GGGGACAAGA AAGCCACGGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329163.1
CDS	573..2861
Translation	MYPQGRHPVPLQPGQSFKFTVLETLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYIMYYEMSYGLNIEMHKQAEIVKRLSAICAQIIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQHLSHHTPGIPLTPHPSGLSLGGTSSLLALTGALGVTAHLANKDERNHLDPEHLREGAPSRSKSVSSTDSQAVEDRQGPSGVYSSQAGGDAKRRRCEEKDILQSHYDSDGDKSEDNLVVDVSNEEASSPVGSAPLSPHGNGLDRALPLRKELPGSPSPPTASPPHSPTPGKNKDTAQTEKSQSPSSKSGNSSPSHHDPLTPGPPGPPGPSTSCLRLVNKPASSADPLALRSPLTMTPGSYPAHFGVVSHAGLNGELASPGGFGGSLTLSPQISAAASAYTRSPMVAYESRSHLRPSGLSSSLPGSTSGKPAYSFHVSADGQMQPVPFPPDALLGPGIPRHARQIHTLSHGEVVCAVTISTSTRHVYTGGKGCVKVWDISQPGSKSPMAQLDCLNRDNYIRSCKILPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDNKVCFSCCSDGNIVVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRCWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDVSPDDKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_021473488.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473488.1

ORF Insert Sequence:

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCTCCA GCACCTGTCT CACCACACTC CAGGTATTCC TCTCACTCCT 
CATCCTTCAG GTCTGTCTCT GGGTGGGACG TCCAGTCTGT TGGCCCTCAC TGGGGCCCTG 
GGAGTCACGG CACACCTGGC CAACAAGGAC GAACGCAACC ACCTAGACCC AGAGCACCTA 
CGAGAGGGTG CGCCCAGCAG GAGTAAATCA GTGTCGTCGA CTGACAGTCA GGCGGTGGAG 
GATCGTCAGG GTCCGTCAGG GGTTTATTCC TCTCAGGCCG GTGGTGATGC CAAACGGAGA 
CGCTGTGAGG AAAAAGACAT TCTGCAGTCC CACTACGACA GTGATGGAGA CAAGAGCGAG 
GACAATCTTG TAGTAGACGT CTCGAATGAG GAAGCTTCCT CTCCGGTCGG CAGTGCTCCT 
CTCTCTCCTC ATGGAAATGG ACTGGATCGA GCTCTACCAC TGAGGAAAGA GCTCCCAGGA 
TCCCCCAGTC CCCCCACAGC CTCCCCGCCA CACAGCCCAA CCCCTGGCAA AAACAAGGAC 
ACGGCACAAA CAGAAAAATC TCAGTCGCCT TCCTCAAAAT CAGGCAATTC CTCTCCCTCT 
CACCATGACC CTCTCACCCC TGGTCCACCC GGCCCACCGG GACCCAGCAC GTCCTGTTTA 
CGCCTGGTCA ACAAACCTGC CTCCTCGGCA GACCCTCTGG CTCTGCGGAG CCCCTTAACC 
ATGACGCCCG GTTCTTACCC AGCTCACTTT GGGGTGGTGT CTCACGCTGG GCTGAATGGA 
GAGCTGGCCA GTCCGGGCGG ATTCGGAGGC TCACTCACCC TCTCGCCCCA AATCAGTGCT 
GCAGCCAGTG CCTACACACG CAGCCCTATG GTAGCGTATG AATCCCGTTC ACATCTCAGG 
CCCTCAGGGC TGTCCTCCTC ACTTCCTGGC TCTACTAGTG GCAAACCTGC TTACTCGTTC 
CATGTGAGCG CAGATGGTCA AATGCAGCCG GTGCCCTTTC CTCCTGATGC CCTCCTCGGC 
CCTGGCATCC CGCGTCATGC CCGTCAGATC CACACTCTGA GTCATGGAGA GGTGGTGTGC 
GCCGTCACCA TCAGCACTTC CACACGCCAC GTCTACACCG GTGGCAAAGG TTGTGTCAAA 
GTATGGGACA TCAGCCAACC AGGCAGCAAG AGCCCGATGG CCCAACTGGA CTGTTTGAAT 
CGAGACAACT ACATCCGCTC ATGCAAGATT CTCCCAGATG GCCGGACCTT AATAGTTGGG 
GGCGAGGCCA GCACACTGTC CATCTGGGAC TTGGCCACAC CCACTCCACG CATCAAGGCA 
GAATTGACGT CTTCGGCTCC GGCCTGCTAC GCTCTGGCCA TCAGTCCTGA CAACAAGGTC 
TGCTTCTCCT GCTGCAGTGA CGGAAACATC GTAGTCTGGG ACCTGCACAA CCAGACACTC 
GTCAGGCAGT TCCAAGGCCA CACAGATGGA GCCAGTTGTA TTGATATTTC TAATGATGGG 
ACCAAATTGT GGACGGGAGG TCTGGACAAC ACTGTACGCT GCTGGGACCT GAGAGAAGGG 
CGACAGCTGC AGCAGCATGA CTTCACATCA CAGATCTTTT CTCTGGGTTA CTGTCCTACG 
GGTGAATGGC TTGCAGTGGG GATGGAGAGC AGTAATGTGG AAGTGCTGCA CGTCTCCAAA 
CCAGACAAAT ACCAGCTACA CCTTCATGAA AGCTGCGTTC TCTCGCTCAA GTTTGCCTAC 
TGCGGGAAGT GGTTTGTAAG CACAGGAAAG GACAATCTTC TCAATGCATG GAGGACGCCT 
TACGGAGCCA GTATTTTCCA GTCAAAGGAG ACCTCATCTG TCCTGAGCTG TGACGTGTCT 
CCAGATGACA AGTATATTGT AACTGGATCT GGGGACAAGA AAGCCACGGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-42p8.3 ( XM_005170536.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170593.1 Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26974
Clone ID Related Accession (Same CDS sequence)	XM_021473488.1 , XM_005170536.3 , XM_005170536.4
Accession Version	XM_005170536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transducin-like enhancer protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCTCCA GCACCTGTCT CACCACACTC CAGGTATTCC TCTCACTCCT 
CATCCTTCAG GTCTGTCTCT GGGTGGGACG TCCAGTCTGT TGGCCCTCAC TGGGGCCCTG 
GGAGTCACGG CACACCTGGC CAACAAGGAC GAACGCAACC ACCTAGACCC AGAGCACCTA 
CGAGAGGGTG CGCCCAGCAG GAGTAAATCA GTGTCGTCGA CTGACAGTCA GGCGGTGGAG 
GATCGTCAGG GTCCGTCAGG GGTTTATTCC TCTCAGGCCG GTGGTGATGC CAAACGGAGA 
CGCTGTGAGG AAAAAGACAT TCTGCAGTCC CACTACGACA GTGATGGAGA CAAGAGCGAG 
GACAATCTTG TAGTAGACGT CTCGAATGAG GAAGCTTCCT CTCCGGTCGG CAGTGCTCCT 
CTCTCTCCTC ATGGAAATGG ACTGGATCGA GCTCTACCAC TGAGGAAAGA GCTCCCAGGA 
TCCCCCAGTC CCCCCACAGC CTCCCCGCCA CACAGCCCAA CCCCTGGCAA AAACAAGGAC 
ACGGCACAAA CAGAAAAATC TCAGTCGCCT TCCTCAAAAT CAGGCAATTC CTCTCCCTCT 
CACCATGACC CTCTCACCCC TGGTCCACCC GGCCCACCGG GACCCAGCAC GTCCTGTTTA 
CGCCTGGTCA ACAAACCTGC CTCCTCGGCA GACCCTCTGG CTCTGCGGAG CCCCTTAACC 
ATGACGCCCG GTTCTTACCC AGCTCACTTT GGGGTGGTGT CTCACGCTGG GCTGAATGGA 
GAGCTGGCCA GTCCGGGCGG ATTCGGAGGC TCACTCACCC TCTCGCCCCA AATCAGTGCT 
GCAGCCAGTG CCTACACACG CAGCCCTATG GTAGCGTATG AATCCCGTTC ACATCTCAGG 
CCCTCAGGGC TGTCCTCCTC ACTTCCTGGC TCTACTAGTG GCAAACCTGC TTACTCGTTC 
CATGTGAGCG CAGATGGTCA AATGCAGCCG GTGCCCTTTC CTCCTGATGC CCTCCTCGGC 
CCTGGCATCC CGCGTCATGC CCGTCAGATC CACACTCTGA GTCATGGAGA GGTGGTGTGC 
GCCGTCACCA TCAGCACTTC CACACGCCAC GTCTACACCG GTGGCAAAGG TTGTGTCAAA 
GTATGGGACA TCAGCCAACC AGGCAGCAAG AGCCCGATGG CCCAACTGGA CTGTTTGAAT 
CGAGACAACT ACATCCGCTC ATGCAAGATT CTCCCAGATG GCCGGACCTT AATAGTTGGG 
GGCGAGGCCA GCACACTGTC CATCTGGGAC TTGGCCACAC CCACTCCACG CATCAAGGCA 
GAATTGACGT CTTCGGCTCC GGCCTGCTAC GCTCTGGCCA TCAGTCCTGA CAACAAGGTC 
TGCTTCTCCT GCTGCAGTGA CGGAAACATC GTAGTCTGGG ACCTGCACAA CCAGACACTC 
GTCAGGCAGT TCCAAGGCCA CACAGATGGA GCCAGTTGTA TTGATATTTC TAATGATGGG 
ACCAAATTGT GGACGGGAGG TCTGGACAAC ACTGTACGCT GCTGGGACCT GAGAGAAGGG 
CGACAGCTGC AGCAGCATGA CTTCACATCA CAGATCTTTT CTCTGGGTTA CTGTCCTACG 
GGTGAATGGC TTGCAGTGGG GATGGAGAGC AGTAATGTGG AAGTGCTGCA CGTCTCCAAA 
CCAGACAAAT ACCAGCTACA CCTTCATGAA AGCTGCGTTC TCTCGCTCAA GTTTGCCTAC 
TGCGGGAAGT GGTTTGTAAG CACAGGAAAG GACAATCTTC TCAATGCATG GAGGACGCCT 
TACGGAGCCA GTATTTTCCA GTCAAAGGAG ACCTCATCTG TCCTGAGCTG TGACGTGTCT 
CCAGATGACA AGTATATTGT AACTGGATCT GGGGACAAGA AAGCCACGGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170593.1
CDS	579..2867
Translation	MYPQGRHPVPLQPGQSFKFTVLETLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYIMYYEMSYGLNIEMHKQAEIVKRLSAICAQIIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQHLSHHTPGIPLTPHPSGLSLGGTSSLLALTGALGVTAHLANKDERNHLDPEHLREGAPSRSKSVSSTDSQAVEDRQGPSGVYSSQAGGDAKRRRCEEKDILQSHYDSDGDKSEDNLVVDVSNEEASSPVGSAPLSPHGNGLDRALPLRKELPGSPSPPTASPPHSPTPGKNKDTAQTEKSQSPSSKSGNSSPSHHDPLTPGPPGPPGPSTSCLRLVNKPASSADPLALRSPLTMTPGSYPAHFGVVSHAGLNGELASPGGFGGSLTLSPQISAAASAYTRSPMVAYESRSHLRPSGLSSSLPGSTSGKPAYSFHVSADGQMQPVPFPPDALLGPGIPRHARQIHTLSHGEVVCAVTISTSTRHVYTGGKGCVKVWDISQPGSKSPMAQLDCLNRDNYIRSCKILPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDNKVCFSCCSDGNIVVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRCWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDVSPDDKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_005170536.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170536.3

ORF Insert Sequence:

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCTCCA GCACCTGTCT CACCACACTC CAGGTATTCC TCTCACTCCT 
CATCCTTCAG GTCTGTCTCT GGGTGGGACG TCCAGTCTGT TGGCCCTCAC TGGGGCCCTG 
GGAGTCACGG CACACCTGGC CAACAAGGAC GAACGCAACC ACCTAGACCC AGAGCACCTA 
CGAGAGGGTG CGCCCAGCAG GAGTAAATCA GTGTCGTCGA CTGACAGTCA GGCGGTGGAG 
GATCGTCAGG GTCCGTCAGG GGTTTATTCC TCTCAGGCCG GTGGTGATGC CAAACGGAGA 
CGCTGTGAGG AAAAAGACAT TCTGCAGTCC CACTACGACA GTGATGGAGA CAAGAGCGAG 
GACAATCTTG TAGTAGACGT CTCGAATGAG GAAGCTTCCT CTCCGGTCGG CAGTGCTCCT 
CTCTCTCCTC ATGGAAATGG ACTGGATCGA GCTCTACCAC TGAGGAAAGA GCTCCCAGGA 
TCCCCCAGTC CCCCCACAGC CTCCCCGCCA CACAGCCCAA CCCCTGGCAA AAACAAGGAC 
ACGGCACAAA CAGAAAAATC TCAGTCGCCT TCCTCAAAAT CAGGCAATTC CTCTCCCTCT 
CACCATGACC CTCTCACCCC TGGTCCACCC GGCCCACCGG GACCCAGCAC GTCCTGTTTA 
CGCCTGGTCA ACAAACCTGC CTCCTCGGCA GACCCTCTGG CTCTGCGGAG CCCCTTAACC 
ATGACGCCCG GTTCTTACCC AGCTCACTTT GGGGTGGTGT CTCACGCTGG GCTGAATGGA 
GAGCTGGCCA GTCCGGGCGG ATTCGGAGGC TCACTCACCC TCTCGCCCCA AATCAGTGCT 
GCAGCCAGTG CCTACACACG CAGCCCTATG GTAGCGTATG AATCCCGTTC ACATCTCAGG 
CCCTCAGGGC TGTCCTCCTC ACTTCCTGGC TCTACTAGTG GCAAACCTGC TTACTCGTTC 
CATGTGAGCG CAGATGGTCA AATGCAGCCG GTGCCCTTTC CTCCTGATGC CCTCCTCGGC 
CCTGGCATCC CGCGTCATGC CCGTCAGATC CACACTCTGA GTCATGGAGA GGTGGTGTGC 
GCCGTCACCA TCAGCACTTC CACACGCCAC GTCTACACCG GTGGCAAAGG TTGTGTCAAA 
GTATGGGACA TCAGCCAACC AGGCAGCAAG AGCCCGATGG CCCAACTGGA CTGTTTGAAT 
CGAGACAACT ACATCCGCTC ATGCAAGATT CTCCCAGATG GCCGGACCTT AATAGTTGGG 
GGCGAGGCCA GCACACTGTC CATCTGGGAC TTGGCCACAC CCACTCCACG CATCAAGGCA 
GAATTGACGT CTTCGGCTCC GGCCTGCTAC GCTCTGGCCA TCAGTCCTGA CAACAAGGTC 
TGCTTCTCCT GCTGCAGTGA CGGAAACATC GTAGTCTGGG ACCTGCACAA CCAGACACTC 
GTCAGGCAGT TCCAAGGCCA CACAGATGGA GCCAGTTGTA TTGATATTTC TAATGATGGG 
ACCAAATTGT GGACGGGAGG TCTGGACAAC ACTGTACGCT GCTGGGACCT GAGAGAAGGG 
CGACAGCTGC AGCAGCATGA CTTCACATCA CAGATCTTTT CTCTGGGTTA CTGTCCTACG 
GGTGAATGGC TTGCAGTGGG GATGGAGAGC AGTAATGTGG AAGTGCTGCA CGTCTCCAAA 
CCAGACAAAT ACCAGCTACA CCTTCATGAA AGCTGCGTTC TCTCGCTCAA GTTTGCCTAC 
TGCGGGAAGT GGTTTGTAAG CACAGGAAAG GACAATCTTC TCAATGCATG GAGGACGCCT 
TACGGAGCCA GTATTTTCCA GTCAAAGGAG ACCTCATCTG TCCTGAGCTG TGACGTGTCT 
CCAGATGACA AGTATATTGT AACTGGATCT GGGGACAAGA AAGCCACGGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-42p8.3 ( XM_005170536.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170593.1 Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26974
Clone ID Related Accession (Same CDS sequence)	XM_021473488.1 , XM_005170536.3 , XM_005170536.4
Accession Version	XM_005170536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transducin-like enhancer protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170536.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCTCCA GCACCTGTCT CACCACACTC CAGGTATTCC TCTCACTCCT 
CATCCTTCAG GTCTGTCTCT GGGTGGGACG TCCAGTCTGT TGGCCCTCAC TGGGGCCCTG 
GGAGTCACGG CACACCTGGC CAACAAGGAC GAACGCAACC ACCTAGACCC AGAGCACCTA 
CGAGAGGGTG CGCCCAGCAG GAGTAAATCA GTGTCGTCGA CTGACAGTCA GGCGGTGGAG 
GATCGTCAGG GTCCGTCAGG GGTTTATTCC TCTCAGGCCG GTGGTGATGC CAAACGGAGA 
CGCTGTGAGG AAAAAGACAT TCTGCAGTCC CACTACGACA GTGATGGAGA CAAGAGCGAG 
GACAATCTTG TAGTAGACGT CTCGAATGAG GAAGCTTCCT CTCCGGTCGG CAGTGCTCCT 
CTCTCTCCTC ATGGAAATGG ACTGGATCGA GCTCTACCAC TGAGGAAAGA GCTCCCAGGA 
TCCCCCAGTC CCCCCACAGC CTCCCCGCCA CACAGCCCAA CCCCTGGCAA AAACAAGGAC 
ACGGCACAAA CAGAAAAATC TCAGTCGCCT TCCTCAAAAT CAGGCAATTC CTCTCCCTCT 
CACCATGACC CTCTCACCCC TGGTCCACCC GGCCCACCGG GACCCAGCAC GTCCTGTTTA 
CGCCTGGTCA ACAAACCTGC CTCCTCGGCA GACCCTCTGG CTCTGCGGAG CCCCTTAACC 
ATGACGCCCG GTTCTTACCC AGCTCACTTT GGGGTGGTGT CTCACGCTGG GCTGAATGGA 
GAGCTGGCCA GTCCGGGCGG ATTCGGAGGC TCACTCACCC TCTCGCCCCA AATCAGTGCT 
GCAGCCAGTG CCTACACACG CAGCCCTATG GTAGCGTATG AATCCCGTTC ACATCTCAGG 
CCCTCAGGGC TGTCCTCCTC ACTTCCTGGC TCTACTAGTG GCAAACCTGC TTACTCGTTC 
CATGTGAGCG CAGATGGTCA AATGCAGCCG GTGCCCTTTC CTCCTGATGC CCTCCTCGGC 
CCTGGCATCC CGCGTCATGC CCGTCAGATC CACACTCTGA GTCATGGAGA GGTGGTGTGC 
GCCGTCACCA TCAGCACTTC CACACGCCAC GTCTACACCG GTGGCAAAGG TTGTGTCAAA 
GTATGGGACA TCAGCCAACC AGGCAGCAAG AGCCCGATGG CCCAACTGGA CTGTTTGAAT 
CGAGACAACT ACATCCGCTC ATGCAAGATT CTCCCAGATG GCCGGACCTT AATAGTTGGG 
GGCGAGGCCA GCACACTGTC CATCTGGGAC TTGGCCACAC CCACTCCACG CATCAAGGCA 
GAATTGACGT CTTCGGCTCC GGCCTGCTAC GCTCTGGCCA TCAGTCCTGA CAACAAGGTC 
TGCTTCTCCT GCTGCAGTGA CGGAAACATC GTAGTCTGGG ACCTGCACAA CCAGACACTC 
GTCAGGCAGT TCCAAGGCCA CACAGATGGA GCCAGTTGTA TTGATATTTC TAATGATGGG 
ACCAAATTGT GGACGGGAGG TCTGGACAAC ACTGTACGCT GCTGGGACCT GAGAGAAGGG 
CGACAGCTGC AGCAGCATGA CTTCACATCA CAGATCTTTT CTCTGGGTTA CTGTCCTACG 
GGTGAATGGC TTGCAGTGGG GATGGAGAGC AGTAATGTGG AAGTGCTGCA CGTCTCCAAA 
CCAGACAAAT ACCAGCTACA CCTTCATGAA AGCTGCGTTC TCTCGCTCAA GTTTGCCTAC 
TGCGGGAAGT GGTTTGTAAG CACAGGAAAG GACAATCTTC TCAATGCATG GAGGACGCCT 
TACGGAGCCA GTATTTTCCA GTCAAAGGAG ACCTCATCTG TCCTGAGCTG TGACGTGTCT 
CCAGATGACA AGTATATTGT AACTGGATCT GGGGACAAGA AAGCCACGGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170593.1
CDS	572..2860
Translation	MYPQGRHPVPLQPGQSFKFTVLETLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYIMYYEMSYGLNIEMHKQAEIVKRLSAICAQIIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQHLSHHTPGIPLTPHPSGLSLGGTSSLLALTGALGVTAHLANKDERNHLDPEHLREGAPSRSKSVSSTDSQAVEDRQGPSGVYSSQAGGDAKRRRCEEKDILQSHYDSDGDKSEDNLVVDVSNEEASSPVGSAPLSPHGNGLDRALPLRKELPGSPSPPTASPPHSPTPGKNKDTAQTEKSQSPSSKSGNSSPSHHDPLTPGPPGPPGPSTSCLRLVNKPASSADPLALRSPLTMTPGSYPAHFGVVSHAGLNGELASPGGFGGSLTLSPQISAAASAYTRSPMVAYESRSHLRPSGLSSSLPGSTSGKPAYSFHVSADGQMQPVPFPPDALLGPGIPRHARQIHTLSHGEVVCAVTISTSTRHVYTGGKGCVKVWDISQPGSKSPMAQLDCLNRDNYIRSCKILPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDNKVCFSCCSDGNIVVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRCWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDVSPDDKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_005170536.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170536.4

ORF Insert Sequence:

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCTCCA GCACCTGTCT CACCACACTC CAGGTATTCC TCTCACTCCT 
CATCCTTCAG GTCTGTCTCT GGGTGGGACG TCCAGTCTGT TGGCCCTCAC TGGGGCCCTG 
GGAGTCACGG CACACCTGGC CAACAAGGAC GAACGCAACC ACCTAGACCC AGAGCACCTA 
CGAGAGGGTG CGCCCAGCAG GAGTAAATCA GTGTCGTCGA CTGACAGTCA GGCGGTGGAG 
GATCGTCAGG GTCCGTCAGG GGTTTATTCC TCTCAGGCCG GTGGTGATGC CAAACGGAGA 
CGCTGTGAGG AAAAAGACAT TCTGCAGTCC CACTACGACA GTGATGGAGA CAAGAGCGAG 
GACAATCTTG TAGTAGACGT CTCGAATGAG GAAGCTTCCT CTCCGGTCGG CAGTGCTCCT 
CTCTCTCCTC ATGGAAATGG ACTGGATCGA GCTCTACCAC TGAGGAAAGA GCTCCCAGGA 
TCCCCCAGTC CCCCCACAGC CTCCCCGCCA CACAGCCCAA CCCCTGGCAA AAACAAGGAC 
ACGGCACAAA CAGAAAAATC TCAGTCGCCT TCCTCAAAAT CAGGCAATTC CTCTCCCTCT 
CACCATGACC CTCTCACCCC TGGTCCACCC GGCCCACCGG GACCCAGCAC GTCCTGTTTA 
CGCCTGGTCA ACAAACCTGC CTCCTCGGCA GACCCTCTGG CTCTGCGGAG CCCCTTAACC 
ATGACGCCCG GTTCTTACCC AGCTCACTTT GGGGTGGTGT CTCACGCTGG GCTGAATGGA 
GAGCTGGCCA GTCCGGGCGG ATTCGGAGGC TCACTCACCC TCTCGCCCCA AATCAGTGCT 
GCAGCCAGTG CCTACACACG CAGCCCTATG GTAGCGTATG AATCCCGTTC ACATCTCAGG 
CCCTCAGGGC TGTCCTCCTC ACTTCCTGGC TCTACTAGTG GCAAACCTGC TTACTCGTTC 
CATGTGAGCG CAGATGGTCA AATGCAGCCG GTGCCCTTTC CTCCTGATGC CCTCCTCGGC 
CCTGGCATCC CGCGTCATGC CCGTCAGATC CACACTCTGA GTCATGGAGA GGTGGTGTGC 
GCCGTCACCA TCAGCACTTC CACACGCCAC GTCTACACCG GTGGCAAAGG TTGTGTCAAA 
GTATGGGACA TCAGCCAACC AGGCAGCAAG AGCCCGATGG CCCAACTGGA CTGTTTGAAT 
CGAGACAACT ACATCCGCTC ATGCAAGATT CTCCCAGATG GCCGGACCTT AATAGTTGGG 
GGCGAGGCCA GCACACTGTC CATCTGGGAC TTGGCCACAC CCACTCCACG CATCAAGGCA 
GAATTGACGT CTTCGGCTCC GGCCTGCTAC GCTCTGGCCA TCAGTCCTGA CAACAAGGTC 
TGCTTCTCCT GCTGCAGTGA CGGAAACATC GTAGTCTGGG ACCTGCACAA CCAGACACTC 
GTCAGGCAGT TCCAAGGCCA CACAGATGGA GCCAGTTGTA TTGATATTTC TAATGATGGG 
ACCAAATTGT GGACGGGAGG TCTGGACAAC ACTGTACGCT GCTGGGACCT GAGAGAAGGG 
CGACAGCTGC AGCAGCATGA CTTCACATCA CAGATCTTTT CTCTGGGTTA CTGTCCTACG 
GGTGAATGGC TTGCAGTGGG GATGGAGAGC AGTAATGTGG AAGTGCTGCA CGTCTCCAAA 
CCAGACAAAT ACCAGCTACA CCTTCATGAA AGCTGCGTTC TCTCGCTCAA GTTTGCCTAC 
TGCGGGAAGT GGTTTGTAAG CACAGGAAAG GACAATCTTC TCAATGCATG GAGGACGCCT 
TACGGAGCCA GTATTTTCCA GTCAAAGGAG ACCTCATCTG TCCTGAGCTG TGACGTGTCT 
CCAGATGACA AGTATATTGT AACTGGATCT GGGGACAAGA AAGCCACGGT GTATGAGGTG 
GTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-42p8.3 ( XM_021473487.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329162.1 Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa26975
Clone ID Related Accession (Same CDS sequence)	XM_021473487.1 , XM_687379.6 , XM_687379.7
Accession Version	XM_021473487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transducin-like enhancer protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395105.1) and transcript sequence (GFIL01022447.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 3521 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCAGCT CCAGCACCTG TCTCACCACA CTCCAGGTAT TCCTCTCACT 
CCTCATCCTT CAGGTCTGTC TCTGGGTGGG ACGTCCAGTC TGTTGGCCCT CACTGGGGCC 
CTGGGAGTCA CGGCACACCT GGCCAACAAG GACGAACGCA ACCACCTAGA CCCAGAGCAC 
CTACGAGAGG GTGCGCCCAG CAGGAGTAAA TCAGTGTCGT CGACTGACAG TCAGGCGGTG 
GAGGATCGTC AGGGTCCGTC AGGGGTTTAT TCCTCTCAGG CCGGTGGTGA TGCCAAACGG 
AGACGCTGTG AGGAAAAAGA CATTCTGCAG TCCCACTACG ACAGTGATGG AGACAAGAGC 
GAGGACAATC TTGTAGTAGA CGTCTCGAAT GAGGAAGCTT CCTCTCCGGT CGGCAGTGCT 
CCTCTCTCTC CTCATGGAAA TGGACTGGAT CGAGCTCTAC CACTGAGGAA AGAGCTCCCA 
GGATCCCCCA GTCCCCCCAC AGCCTCCCCG CCACACAGCC CAACCCCTGG CAAAAACAAG 
GACACGGCAC AAACAGAAAA ATCTCAGTCG CCTTCCTCAA AATCAGGCAA TTCCTCTCCC 
TCTCACCATG ACCCTCTCAC CCCTGGTCCA CCCGGCCCAC CGGGACCCAG CACGTCCTGT 
TTACGCCTGG TCAACAAACC TGCCTCCTCG GCAGACCCTC TGGCTCTGCG GAGCCCCTTA 
ACCATGACGC CCGGTTCTTA CCCAGCTCAC TTTGGGGTGG TGTCTCACGC TGGGCTGAAT 
GGAGAGCTGG CCAGTCCGGG CGGATTCGGA GGCTCACTCA CCCTCTCGCC CCAAATCAGT 
GCTGCAGCCA GTGCCTACAC ACGCAGCCCT ATGGTAGCGT ATGAATCCCG TTCACATCTC 
AGGCCCTCAG GGCTGTCCTC CTCACTTCCT GGCTCTACTA GTGGCAAACC TGCTTACTCG 
TTCCATGTGA GCGCAGATGG TCAAATGCAG CCGGTGCCCT TTCCTCCTGA TGCCCTCCTC 
GGCCCTGGCA TCCCGCGTCA TGCCCGTCAG ATCCACACTC TGAGTCATGG AGAGGTGGTG 
TGCGCCGTCA CCATCAGCAC TTCCACACGC CACGTCTACA CCGGTGGCAA AGGTTGTGTC 
AAAGTATGGG ACATCAGCCA ACCAGGCAGC AAGAGCCCGA TGGCCCAACT GGACTGTTTG 
AATCGAGACA ACTACATCCG CTCATGCAAG ATTCTCCCAG ATGGCCGGAC CTTAATAGTT 
GGGGGCGAGG CCAGCACACT GTCCATCTGG GACTTGGCCA CACCCACTCC ACGCATCAAG 
GCAGAATTGA CGTCTTCGGC TCCGGCCTGC TACGCTCTGG CCATCAGTCC TGACAACAAG 
GTCTGCTTCT CCTGCTGCAG TGACGGAAAC ATCGTAGTCT GGGACCTGCA CAACCAGACA 
CTCGTCAGGC AGTTCCAAGG CCACACAGAT GGAGCCAGTT GTATTGATAT TTCTAATGAT 
GGGACCAAAT TGTGGACGGG AGGTCTGGAC AACACTGTAC GCTGCTGGGA CCTGAGAGAA 
GGGCGACAGC TGCAGCAGCA TGACTTCACA TCACAGATCT TTTCTCTGGG TTACTGTCCT 
ACGGGTGAAT GGCTTGCAGT GGGGATGGAG AGCAGTAATG TGGAAGTGCT GCACGTCTCC 
AAACCAGACA AATACCAGCT ACACCTTCAT GAAAGCTGCG TTCTCTCGCT CAAGTTTGCC 
TACTGCGGGA AGTGGTTTGT AAGCACAGGA AAGGACAATC TTCTCAATGC ATGGAGGACG 
CCTTACGGAG CCAGTATTTT CCAGTCAAAG GAGACCTCAT CTGTCCTGAG CTGTGACGTG 
TCTCCAGATG ACAAGTATAT TGTAACTGGA TCTGGGGACA AGAAAGCCAC GGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329162.1
CDS	573..2864
Translation	MYPQGRHPVPLQPGQSFKFTVLETLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYIMYYEMSYGLNIEMHKQAEIVKRLSAICAQIIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQQLQHLSHHTPGIPLTPHPSGLSLGGTSSLLALTGALGVTAHLANKDERNHLDPEHLREGAPSRSKSVSSTDSQAVEDRQGPSGVYSSQAGGDAKRRRCEEKDILQSHYDSDGDKSEDNLVVDVSNEEASSPVGSAPLSPHGNGLDRALPLRKELPGSPSPPTASPPHSPTPGKNKDTAQTEKSQSPSSKSGNSSPSHHDPLTPGPPGPPGPSTSCLRLVNKPASSADPLALRSPLTMTPGSYPAHFGVVSHAGLNGELASPGGFGGSLTLSPQISAAASAYTRSPMVAYESRSHLRPSGLSSSLPGSTSGKPAYSFHVSADGQMQPVPFPPDALLGPGIPRHARQIHTLSHGEVVCAVTISTSTRHVYTGGKGCVKVWDISQPGSKSPMAQLDCLNRDNYIRSCKILPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDNKVCFSCCSDGNIVVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRCWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDVSPDDKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_021473487.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473487.1

ORF Insert Sequence:

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCAGCT CCAGCACCTG TCTCACCACA CTCCAGGTAT TCCTCTCACT 
CCTCATCCTT CAGGTCTGTC TCTGGGTGGG ACGTCCAGTC TGTTGGCCCT CACTGGGGCC 
CTGGGAGTCA CGGCACACCT GGCCAACAAG GACGAACGCA ACCACCTAGA CCCAGAGCAC 
CTACGAGAGG GTGCGCCCAG CAGGAGTAAA TCAGTGTCGT CGACTGACAG TCAGGCGGTG 
GAGGATCGTC AGGGTCCGTC AGGGGTTTAT TCCTCTCAGG CCGGTGGTGA TGCCAAACGG 
AGACGCTGTG AGGAAAAAGA CATTCTGCAG TCCCACTACG ACAGTGATGG AGACAAGAGC 
GAGGACAATC TTGTAGTAGA CGTCTCGAAT GAGGAAGCTT CCTCTCCGGT CGGCAGTGCT 
CCTCTCTCTC CTCATGGAAA TGGACTGGAT CGAGCTCTAC CACTGAGGAA AGAGCTCCCA 
GGATCCCCCA GTCCCCCCAC AGCCTCCCCG CCACACAGCC CAACCCCTGG CAAAAACAAG 
GACACGGCAC AAACAGAAAA ATCTCAGTCG CCTTCCTCAA AATCAGGCAA TTCCTCTCCC 
TCTCACCATG ACCCTCTCAC CCCTGGTCCA CCCGGCCCAC CGGGACCCAG CACGTCCTGT 
TTACGCCTGG TCAACAAACC TGCCTCCTCG GCAGACCCTC TGGCTCTGCG GAGCCCCTTA 
ACCATGACGC CCGGTTCTTA CCCAGCTCAC TTTGGGGTGG TGTCTCACGC TGGGCTGAAT 
GGAGAGCTGG CCAGTCCGGG CGGATTCGGA GGCTCACTCA CCCTCTCGCC CCAAATCAGT 
GCTGCAGCCA GTGCCTACAC ACGCAGCCCT ATGGTAGCGT ATGAATCCCG TTCACATCTC 
AGGCCCTCAG GGCTGTCCTC CTCACTTCCT GGCTCTACTA GTGGCAAACC TGCTTACTCG 
TTCCATGTGA GCGCAGATGG TCAAATGCAG CCGGTGCCCT TTCCTCCTGA TGCCCTCCTC 
GGCCCTGGCA TCCCGCGTCA TGCCCGTCAG ATCCACACTC TGAGTCATGG AGAGGTGGTG 
TGCGCCGTCA CCATCAGCAC TTCCACACGC CACGTCTACA CCGGTGGCAA AGGTTGTGTC 
AAAGTATGGG ACATCAGCCA ACCAGGCAGC AAGAGCCCGA TGGCCCAACT GGACTGTTTG 
AATCGAGACA ACTACATCCG CTCATGCAAG ATTCTCCCAG ATGGCCGGAC CTTAATAGTT 
GGGGGCGAGG CCAGCACACT GTCCATCTGG GACTTGGCCA CACCCACTCC ACGCATCAAG 
GCAGAATTGA CGTCTTCGGC TCCGGCCTGC TACGCTCTGG CCATCAGTCC TGACAACAAG 
GTCTGCTTCT CCTGCTGCAG TGACGGAAAC ATCGTAGTCT GGGACCTGCA CAACCAGACA 
CTCGTCAGGC AGTTCCAAGG CCACACAGAT GGAGCCAGTT GTATTGATAT TTCTAATGAT 
GGGACCAAAT TGTGGACGGG AGGTCTGGAC AACACTGTAC GCTGCTGGGA CCTGAGAGAA 
GGGCGACAGC TGCAGCAGCA TGACTTCACA TCACAGATCT TTTCTCTGGG TTACTGTCCT 
ACGGGTGAAT GGCTTGCAGT GGGGATGGAG AGCAGTAATG TGGAAGTGCT GCACGTCTCC 
AAACCAGACA AATACCAGCT ACACCTTCAT GAAAGCTGCG TTCTCTCGCT CAAGTTTGCC 
TACTGCGGGA AGTGGTTTGT AAGCACAGGA AAGGACAATC TTCTCAATGC ATGGAGGACG 
CCTTACGGAG CCAGTATTTT CCAGTCAAAG GAGACCTCAT CTGTCCTGAG CTGTGACGTG 
TCTCCAGATG ACAAGTATAT TGTAACTGGA TCTGGGGACA AGAAAGCCAC GGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-42p8.3 ( XM_687379.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692471.4 Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26975
Clone ID Related Accession (Same CDS sequence)	XM_021473487.1 , XM_687379.6 , XM_687379.7
Accession Version	XM_687379.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transducin-like enhancer protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_687379.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCAGCT CCAGCACCTG TCTCACCACA CTCCAGGTAT TCCTCTCACT 
CCTCATCCTT CAGGTCTGTC TCTGGGTGGG ACGTCCAGTC TGTTGGCCCT CACTGGGGCC 
CTGGGAGTCA CGGCACACCT GGCCAACAAG GACGAACGCA ACCACCTAGA CCCAGAGCAC 
CTACGAGAGG GTGCGCCCAG CAGGAGTAAA TCAGTGTCGT CGACTGACAG TCAGGCGGTG 
GAGGATCGTC AGGGTCCGTC AGGGGTTTAT TCCTCTCAGG CCGGTGGTGA TGCCAAACGG 
AGACGCTGTG AGGAAAAAGA CATTCTGCAG TCCCACTACG ACAGTGATGG AGACAAGAGC 
GAGGACAATC TTGTAGTAGA CGTCTCGAAT GAGGAAGCTT CCTCTCCGGT CGGCAGTGCT 
CCTCTCTCTC CTCATGGAAA TGGACTGGAT CGAGCTCTAC CACTGAGGAA AGAGCTCCCA 
GGATCCCCCA GTCCCCCCAC AGCCTCCCCG CCACACAGCC CAACCCCTGG CAAAAACAAG 
GACACGGCAC AAACAGAAAA ATCTCAGTCG CCTTCCTCAA AATCAGGCAA TTCCTCTCCC 
TCTCACCATG ACCCTCTCAC CCCTGGTCCA CCCGGCCCAC CGGGACCCAG CACGTCCTGT 
TTACGCCTGG TCAACAAACC TGCCTCCTCG GCAGACCCTC TGGCTCTGCG GAGCCCCTTA 
ACCATGACGC CCGGTTCTTA CCCAGCTCAC TTTGGGGTGG TGTCTCACGC TGGGCTGAAT 
GGAGAGCTGG CCAGTCCGGG CGGATTCGGA GGCTCACTCA CCCTCTCGCC CCAAATCAGT 
GCTGCAGCCA GTGCCTACAC ACGCAGCCCT ATGGTAGCGT ATGAATCCCG TTCACATCTC 
AGGCCCTCAG GGCTGTCCTC CTCACTTCCT GGCTCTACTA GTGGCAAACC TGCTTACTCG 
TTCCATGTGA GCGCAGATGG TCAAATGCAG CCGGTGCCCT TTCCTCCTGA TGCCCTCCTC 
GGCCCTGGCA TCCCGCGTCA TGCCCGTCAG ATCCACACTC TGAGTCATGG AGAGGTGGTG 
TGCGCCGTCA CCATCAGCAC TTCCACACGC CACGTCTACA CCGGTGGCAA AGGTTGTGTC 
AAAGTATGGG ACATCAGCCA ACCAGGCAGC AAGAGCCCGA TGGCCCAACT GGACTGTTTG 
AATCGAGACA ACTACATCCG CTCATGCAAG ATTCTCCCAG ATGGCCGGAC CTTAATAGTT 
GGGGGCGAGG CCAGCACACT GTCCATCTGG GACTTGGCCA CACCCACTCC ACGCATCAAG 
GCAGAATTGA CGTCTTCGGC TCCGGCCTGC TACGCTCTGG CCATCAGTCC TGACAACAAG 
GTCTGCTTCT CCTGCTGCAG TGACGGAAAC ATCGTAGTCT GGGACCTGCA CAACCAGACA 
CTCGTCAGGC AGTTCCAAGG CCACACAGAT GGAGCCAGTT GTATTGATAT TTCTAATGAT 
GGGACCAAAT TGTGGACGGG AGGTCTGGAC AACACTGTAC GCTGCTGGGA CCTGAGAGAA 
GGGCGACAGC TGCAGCAGCA TGACTTCACA TCACAGATCT TTTCTCTGGG TTACTGTCCT 
ACGGGTGAAT GGCTTGCAGT GGGGATGGAG AGCAGTAATG TGGAAGTGCT GCACGTCTCC 
AAACCAGACA AATACCAGCT ACACCTTCAT GAAAGCTGCG TTCTCTCGCT CAAGTTTGCC 
TACTGCGGGA AGTGGTTTGT AAGCACAGGA AAGGACAATC TTCTCAATGC ATGGAGGACG 
CCTTACGGAG CCAGTATTTT CCAGTCAAAG GAGACCTCAT CTGTCCTGAG CTGTGACGTG 
TCTCCAGATG ACAAGTATAT TGTAACTGGA TCTGGGGACA AGAAAGCCAC GGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692471.4
CDS	579..2870
Translation	MYPQGRHPVPLQPGQSFKFTVLETLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYIMYYEMSYGLNIEMHKQAEIVKRLSAICAQIIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQQLQHLSHHTPGIPLTPHPSGLSLGGTSSLLALTGALGVTAHLANKDERNHLDPEHLREGAPSRSKSVSSTDSQAVEDRQGPSGVYSSQAGGDAKRRRCEEKDILQSHYDSDGDKSEDNLVVDVSNEEASSPVGSAPLSPHGNGLDRALPLRKELPGSPSPPTASPPHSPTPGKNKDTAQTEKSQSPSSKSGNSSPSHHDPLTPGPPGPPGPSTSCLRLVNKPASSADPLALRSPLTMTPGSYPAHFGVVSHAGLNGELASPGGFGGSLTLSPQISAAASAYTRSPMVAYESRSHLRPSGLSSSLPGSTSGKPAYSFHVSADGQMQPVPFPPDALLGPGIPRHARQIHTLSHGEVVCAVTISTSTRHVYTGGKGCVKVWDISQPGSKSPMAQLDCLNRDNYIRSCKILPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDNKVCFSCCSDGNIVVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRCWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDVSPDDKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_687379.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687379.6

ORF Insert Sequence:

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCAGCT CCAGCACCTG TCTCACCACA CTCCAGGTAT TCCTCTCACT 
CCTCATCCTT CAGGTCTGTC TCTGGGTGGG ACGTCCAGTC TGTTGGCCCT CACTGGGGCC 
CTGGGAGTCA CGGCACACCT GGCCAACAAG GACGAACGCA ACCACCTAGA CCCAGAGCAC 
CTACGAGAGG GTGCGCCCAG CAGGAGTAAA TCAGTGTCGT CGACTGACAG TCAGGCGGTG 
GAGGATCGTC AGGGTCCGTC AGGGGTTTAT TCCTCTCAGG CCGGTGGTGA TGCCAAACGG 
AGACGCTGTG AGGAAAAAGA CATTCTGCAG TCCCACTACG ACAGTGATGG AGACAAGAGC 
GAGGACAATC TTGTAGTAGA CGTCTCGAAT GAGGAAGCTT CCTCTCCGGT CGGCAGTGCT 
CCTCTCTCTC CTCATGGAAA TGGACTGGAT CGAGCTCTAC CACTGAGGAA AGAGCTCCCA 
GGATCCCCCA GTCCCCCCAC AGCCTCCCCG CCACACAGCC CAACCCCTGG CAAAAACAAG 
GACACGGCAC AAACAGAAAA ATCTCAGTCG CCTTCCTCAA AATCAGGCAA TTCCTCTCCC 
TCTCACCATG ACCCTCTCAC CCCTGGTCCA CCCGGCCCAC CGGGACCCAG CACGTCCTGT 
TTACGCCTGG TCAACAAACC TGCCTCCTCG GCAGACCCTC TGGCTCTGCG GAGCCCCTTA 
ACCATGACGC CCGGTTCTTA CCCAGCTCAC TTTGGGGTGG TGTCTCACGC TGGGCTGAAT 
GGAGAGCTGG CCAGTCCGGG CGGATTCGGA GGCTCACTCA CCCTCTCGCC CCAAATCAGT 
GCTGCAGCCA GTGCCTACAC ACGCAGCCCT ATGGTAGCGT ATGAATCCCG TTCACATCTC 
AGGCCCTCAG GGCTGTCCTC CTCACTTCCT GGCTCTACTA GTGGCAAACC TGCTTACTCG 
TTCCATGTGA GCGCAGATGG TCAAATGCAG CCGGTGCCCT TTCCTCCTGA TGCCCTCCTC 
GGCCCTGGCA TCCCGCGTCA TGCCCGTCAG ATCCACACTC TGAGTCATGG AGAGGTGGTG 
TGCGCCGTCA CCATCAGCAC TTCCACACGC CACGTCTACA CCGGTGGCAA AGGTTGTGTC 
AAAGTATGGG ACATCAGCCA ACCAGGCAGC AAGAGCCCGA TGGCCCAACT GGACTGTTTG 
AATCGAGACA ACTACATCCG CTCATGCAAG ATTCTCCCAG ATGGCCGGAC CTTAATAGTT 
GGGGGCGAGG CCAGCACACT GTCCATCTGG GACTTGGCCA CACCCACTCC ACGCATCAAG 
GCAGAATTGA CGTCTTCGGC TCCGGCCTGC TACGCTCTGG CCATCAGTCC TGACAACAAG 
GTCTGCTTCT CCTGCTGCAG TGACGGAAAC ATCGTAGTCT GGGACCTGCA CAACCAGACA 
CTCGTCAGGC AGTTCCAAGG CCACACAGAT GGAGCCAGTT GTATTGATAT TTCTAATGAT 
GGGACCAAAT TGTGGACGGG AGGTCTGGAC AACACTGTAC GCTGCTGGGA CCTGAGAGAA 
GGGCGACAGC TGCAGCAGCA TGACTTCACA TCACAGATCT TTTCTCTGGG TTACTGTCCT 
ACGGGTGAAT GGCTTGCAGT GGGGATGGAG AGCAGTAATG TGGAAGTGCT GCACGTCTCC 
AAACCAGACA AATACCAGCT ACACCTTCAT GAAAGCTGCG TTCTCTCGCT CAAGTTTGCC 
TACTGCGGGA AGTGGTTTGT AAGCACAGGA AAGGACAATC TTCTCAATGC ATGGAGGACG 
CCTTACGGAG CCAGTATTTT CCAGTCAAAG GAGACCTCAT CTGTCCTGAG CTGTGACGTG 
TCTCCAGATG ACAAGTATAT TGTAACTGGA TCTGGGGACA AGAAAGCCAC GGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-42p8.3 ( XM_687379.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692471.4 Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X1, mRNA.

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CloneID	ODa26975
Clone ID Related Accession (Same CDS sequence)	XM_021473487.1 , XM_687379.6 , XM_687379.7
Accession Version	XM_687379.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transducin-like enhancer protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_687379.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCAGCT CCAGCACCTG TCTCACCACA CTCCAGGTAT TCCTCTCACT 
CCTCATCCTT CAGGTCTGTC TCTGGGTGGG ACGTCCAGTC TGTTGGCCCT CACTGGGGCC 
CTGGGAGTCA CGGCACACCT GGCCAACAAG GACGAACGCA ACCACCTAGA CCCAGAGCAC 
CTACGAGAGG GTGCGCCCAG CAGGAGTAAA TCAGTGTCGT CGACTGACAG TCAGGCGGTG 
GAGGATCGTC AGGGTCCGTC AGGGGTTTAT TCCTCTCAGG CCGGTGGTGA TGCCAAACGG 
AGACGCTGTG AGGAAAAAGA CATTCTGCAG TCCCACTACG ACAGTGATGG AGACAAGAGC 
GAGGACAATC TTGTAGTAGA CGTCTCGAAT GAGGAAGCTT CCTCTCCGGT CGGCAGTGCT 
CCTCTCTCTC CTCATGGAAA TGGACTGGAT CGAGCTCTAC CACTGAGGAA AGAGCTCCCA 
GGATCCCCCA GTCCCCCCAC AGCCTCCCCG CCACACAGCC CAACCCCTGG CAAAAACAAG 
GACACGGCAC AAACAGAAAA ATCTCAGTCG CCTTCCTCAA AATCAGGCAA TTCCTCTCCC 
TCTCACCATG ACCCTCTCAC CCCTGGTCCA CCCGGCCCAC CGGGACCCAG CACGTCCTGT 
TTACGCCTGG TCAACAAACC TGCCTCCTCG GCAGACCCTC TGGCTCTGCG GAGCCCCTTA 
ACCATGACGC CCGGTTCTTA CCCAGCTCAC TTTGGGGTGG TGTCTCACGC TGGGCTGAAT 
GGAGAGCTGG CCAGTCCGGG CGGATTCGGA GGCTCACTCA CCCTCTCGCC CCAAATCAGT 
GCTGCAGCCA GTGCCTACAC ACGCAGCCCT ATGGTAGCGT ATGAATCCCG TTCACATCTC 
AGGCCCTCAG GGCTGTCCTC CTCACTTCCT GGCTCTACTA GTGGCAAACC TGCTTACTCG 
TTCCATGTGA GCGCAGATGG TCAAATGCAG CCGGTGCCCT TTCCTCCTGA TGCCCTCCTC 
GGCCCTGGCA TCCCGCGTCA TGCCCGTCAG ATCCACACTC TGAGTCATGG AGAGGTGGTG 
TGCGCCGTCA CCATCAGCAC TTCCACACGC CACGTCTACA CCGGTGGCAA AGGTTGTGTC 
AAAGTATGGG ACATCAGCCA ACCAGGCAGC AAGAGCCCGA TGGCCCAACT GGACTGTTTG 
AATCGAGACA ACTACATCCG CTCATGCAAG ATTCTCCCAG ATGGCCGGAC CTTAATAGTT 
GGGGGCGAGG CCAGCACACT GTCCATCTGG GACTTGGCCA CACCCACTCC ACGCATCAAG 
GCAGAATTGA CGTCTTCGGC TCCGGCCTGC TACGCTCTGG CCATCAGTCC TGACAACAAG 
GTCTGCTTCT CCTGCTGCAG TGACGGAAAC ATCGTAGTCT GGGACCTGCA CAACCAGACA 
CTCGTCAGGC AGTTCCAAGG CCACACAGAT GGAGCCAGTT GTATTGATAT TTCTAATGAT 
GGGACCAAAT TGTGGACGGG AGGTCTGGAC AACACTGTAC GCTGCTGGGA CCTGAGAGAA 
GGGCGACAGC TGCAGCAGCA TGACTTCACA TCACAGATCT TTTCTCTGGG TTACTGTCCT 
ACGGGTGAAT GGCTTGCAGT GGGGATGGAG AGCAGTAATG TGGAAGTGCT GCACGTCTCC 
AAACCAGACA AATACCAGCT ACACCTTCAT GAAAGCTGCG TTCTCTCGCT CAAGTTTGCC 
TACTGCGGGA AGTGGTTTGT AAGCACAGGA AAGGACAATC TTCTCAATGC ATGGAGGACG 
CCTTACGGAG CCAGTATTTT CCAGTCAAAG GAGACCTCAT CTGTCCTGAG CTGTGACGTG 
TCTCCAGATG ACAAGTATAT TGTAACTGGA TCTGGGGACA AGAAAGCCAC GGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692471.4
CDS	572..2863
Translation	MYPQGRHPVPLQPGQSFKFTVLETLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYIMYYEMSYGLNIEMHKQAEIVKRLSAICAQIIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQQLQHLSHHTPGIPLTPHPSGLSLGGTSSLLALTGALGVTAHLANKDERNHLDPEHLREGAPSRSKSVSSTDSQAVEDRQGPSGVYSSQAGGDAKRRRCEEKDILQSHYDSDGDKSEDNLVVDVSNEEASSPVGSAPLSPHGNGLDRALPLRKELPGSPSPPTASPPHSPTPGKNKDTAQTEKSQSPSSKSGNSSPSHHDPLTPGPPGPPGPSTSCLRLVNKPASSADPLALRSPLTMTPGSYPAHFGVVSHAGLNGELASPGGFGGSLTLSPQISAAASAYTRSPMVAYESRSHLRPSGLSSSLPGSTSGKPAYSFHVSADGQMQPVPFPPDALLGPGIPRHARQIHTLSHGEVVCAVTISTSTRHVYTGGKGCVKVWDISQPGSKSPMAQLDCLNRDNYIRSCKILPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDNKVCFSCCSDGNIVVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRCWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKETSSVLSCDVSPDDKYIVTGSGDKKATVYEVVY

Target ORF information:

RefSeq Version	XM_687379.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-42p8.3 (si:dkey-42p8.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687379.7

ORF Insert Sequence:

ATGTACCCAC AGGGTAGACA CCCGGTACCT CTGCAGCCTG GACAGTCATT TAAGTTTACA 
GTTCTGGAGA CCCTGGACCG CATTAAAGAG GAGTTTCAGT TCCTTCAGGC TCAGTACCAC 
AGTCTCAAGT TGGAGTGTGA GAAGTTGGCC AGTGAGAAAA CTGAGATGCA GCGCCACTAT 
ATCATGTACT ACGAGATGTC CTACGGGCTC AACATTGAGA TGCACAAACA GGCGGAGATC 
GTGAAGCGAC TCAGTGCTAT CTGTGCTCAA ATCATCCCTT TCCTCTCTCA GGAGCATCAA 
CAGCAGGTTG TTCAGGCGGT GGAGCGAGCT AAACAAGTGA CCATGGCAGA GCTCAACGCC 
ATCATAGGGC AGCAGCAGCT CCAGCACCTG TCTCACCACA CTCCAGGTAT TCCTCTCACT 
CCTCATCCTT CAGGTCTGTC TCTGGGTGGG ACGTCCAGTC TGTTGGCCCT CACTGGGGCC 
CTGGGAGTCA CGGCACACCT GGCCAACAAG GACGAACGCA ACCACCTAGA CCCAGAGCAC 
CTACGAGAGG GTGCGCCCAG CAGGAGTAAA TCAGTGTCGT CGACTGACAG TCAGGCGGTG 
GAGGATCGTC AGGGTCCGTC AGGGGTTTAT TCCTCTCAGG CCGGTGGTGA TGCCAAACGG 
AGACGCTGTG AGGAAAAAGA CATTCTGCAG TCCCACTACG ACAGTGATGG AGACAAGAGC 
GAGGACAATC TTGTAGTAGA CGTCTCGAAT GAGGAAGCTT CCTCTCCGGT CGGCAGTGCT 
CCTCTCTCTC CTCATGGAAA TGGACTGGAT CGAGCTCTAC CACTGAGGAA AGAGCTCCCA 
GGATCCCCCA GTCCCCCCAC AGCCTCCCCG CCACACAGCC CAACCCCTGG CAAAAACAAG 
GACACGGCAC AAACAGAAAA ATCTCAGTCG CCTTCCTCAA AATCAGGCAA TTCCTCTCCC 
TCTCACCATG ACCCTCTCAC CCCTGGTCCA CCCGGCCCAC CGGGACCCAG CACGTCCTGT 
TTACGCCTGG TCAACAAACC TGCCTCCTCG GCAGACCCTC TGGCTCTGCG GAGCCCCTTA 
ACCATGACGC CCGGTTCTTA CCCAGCTCAC TTTGGGGTGG TGTCTCACGC TGGGCTGAAT 
GGAGAGCTGG CCAGTCCGGG CGGATTCGGA GGCTCACTCA CCCTCTCGCC CCAAATCAGT 
GCTGCAGCCA GTGCCTACAC ACGCAGCCCT ATGGTAGCGT ATGAATCCCG TTCACATCTC 
AGGCCCTCAG GGCTGTCCTC CTCACTTCCT GGCTCTACTA GTGGCAAACC TGCTTACTCG 
TTCCATGTGA GCGCAGATGG TCAAATGCAG CCGGTGCCCT TTCCTCCTGA TGCCCTCCTC 
GGCCCTGGCA TCCCGCGTCA TGCCCGTCAG ATCCACACTC TGAGTCATGG AGAGGTGGTG 
TGCGCCGTCA CCATCAGCAC TTCCACACGC CACGTCTACA CCGGTGGCAA AGGTTGTGTC 
AAAGTATGGG ACATCAGCCA ACCAGGCAGC AAGAGCCCGA TGGCCCAACT GGACTGTTTG 
AATCGAGACA ACTACATCCG CTCATGCAAG ATTCTCCCAG ATGGCCGGAC CTTAATAGTT 
GGGGGCGAGG CCAGCACACT GTCCATCTGG GACTTGGCCA CACCCACTCC ACGCATCAAG 
GCAGAATTGA CGTCTTCGGC TCCGGCCTGC TACGCTCTGG CCATCAGTCC TGACAACAAG 
GTCTGCTTCT CCTGCTGCAG TGACGGAAAC ATCGTAGTCT GGGACCTGCA CAACCAGACA 
CTCGTCAGGC AGTTCCAAGG CCACACAGAT GGAGCCAGTT GTATTGATAT TTCTAATGAT 
GGGACCAAAT TGTGGACGGG AGGTCTGGAC AACACTGTAC GCTGCTGGGA CCTGAGAGAA 
GGGCGACAGC TGCAGCAGCA TGACTTCACA TCACAGATCT TTTCTCTGGG TTACTGTCCT 
ACGGGTGAAT GGCTTGCAGT GGGGATGGAG AGCAGTAATG TGGAAGTGCT GCACGTCTCC 
AAACCAGACA AATACCAGCT ACACCTTCAT GAAAGCTGCG TTCTCTCGCT CAAGTTTGCC 
TACTGCGGGA AGTGGTTTGT AAGCACAGGA AAGGACAATC TTCTCAATGC ATGGAGGACG 
CCTTACGGAG CCAGTATTTT CCAGTCAAAG GAGACCTCAT CTGTCCTGAG CTGTGACGTG 
TCTCCAGATG ACAAGTATAT TGTAACTGGA TCTGGGGACA AGAAAGCCAC GGTGTATGAG 
GTGGTGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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si:dkey-42p8.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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