limch1a cDNA ORF clone, Danio rerio(zebrafish)

The following limch1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the limch1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa147262 XM_021475284.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa00739 NM_001145577.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa05614 XM_005168356.4
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa05616 XM_005168358.4
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa05611 XM_021475267.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa147259 XM_021475270.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa147260 XM_021475273.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa147261 XM_021475278.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa53710 XM_017355096.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa53711 XM_017355097.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa53712 XM_017355098.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa53713 XM_017355100.1
Latest version!
Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
ODa05614 XM_005168356.3 Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa05616 XM_005168358.3 Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa147262
    Clone ID Related Accession (Same CDS sequence) XM_021475284.1
    Accession Version XM_021475284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGATTC AGAGCTACTA GTGTTTCTGA TGAAGCGGAG
    AGCGTGAGTT TGATTGACAT GCGTGATGAA GAGGATGAGC TTGCATACCT TCGGCCGCAC
    AGCCAATCAC GACACGAGCA CCTACACAAC CAGTACAACA AAGTGAAGGA GGAGGAGGAC
    CAGTGGCAGG ACGATTTGGC TCGTTGGAAG AACCGGAGGC GAAGCGCTTC TCAAGATCTG
    ATCAAAAAGG AGGAGGAGAG GAAGATGATG GAGAAGCTAA TGACAACGGA TGGAGGACAC
    GGACATCGCA AGAAGAGCAT CAAAACATAC AAAGAAATTG TAGAGGAGAA AGAGAAAAGG
    GAACAGGAGC TGCATGAACA ATACTGTAAG GCCTCCAGTC CAGAAGAGAA AGCAGCTGTG
    CTTCAGCGAT ATGCTCTCCG TTTCACCATC AGCGATGCCA TACTGGAGAA ACTCCAGCTC
    CCCAAACTGC CTTCTGCCGC CTCACCGATG CACACGTCCC CCCTCCATCA GCCCGAGCAA
    AAGACCACAT CCACTACATT AGTAGAAGCA GAGGTTCCAG AACTTTCACA AACTACCCAA
    ACACAACAGA CTGTCCATGT AAAGCAGAAG ATTACACATG ATGAGGCACC GGCACAGCCT
    AAAATCCCCA AACCTACACC ACCTCCCAAG TGCATCAAAG CTCCAGAAGT CCTGTCGGCA
    CCTTCACGAG TTCCTCCAGC TCCATCAAAG CCTGTACCCC TGATCACCCC CAAACCCTAC
    AGCCAGCCCA AATTCAGCCA AGGCTTCCGC AAATCGTTGA AGAGTGATGG GCTGATACGT
    GTGAACGGAG ACCACAATCA GCAGGTGATA AGAGAGAAGG CTAATAGAGA TCCTCTGCAG
    AGCCCTGAAG TCAACTTCTC TTCCTCACCT CCAAAGCCAG AAATTCAAAG TCAACAGAAT
    GAGGATCCAA TCGTGTTAGA AACCACAGAC CACCAGCAAG ACCTACAGAC ACCTAAACCT
    GACCTGGAGA AAGAAGTCAT AAAGAAGGAG GAAGAAAGTC TGCTTAGTCC TCAGAAAGAG
    ATCAGCAAAG AAGACACCTC AACTACACTT TCAGTGTCAG AACCAGCAGG AAAGCAGCCA
    GTGCCTGACA CAGTGATGAA GCGCAACTTT GTTGTCACAA CAACCATAGT GACAGAACTC
    ACTCAGACGC ACCCCGTCCC ACTAGAGGAC ACGTCCAGCA CAGAGCAGCC TGATACAGTC
    CAAAATACAT GTGATCTGAG CTCAACTGCA GAGTCCTGTA AAGCTGATCA GTCTTCATAC
    TCGATCTCGT CGACAGAGGG GATCGACTAC TCCATTGAAT GTATTGAAAC CCCAATGTTG
    AACCTTGCAA AAAGGGTTGA CCACTGGACG TGGGATCCCA ATGAGGAGCG CAGGCGGCAG
    GAGAGGTGGC AGCAGGAGCA GGAGCGTCTC CTGCAGGAGA AATATCAGCG CGAGCAGGAG
    AAACTGAAGG AGGAATGGGA GAAAGCCCAG AGAGAAGTAG AAGAGGAAGA AAGGAGACAC
    CATGAAGAGG AGAGACGAAT TCTGGAGGAG ACAGTGACCC CGCTGACCCC TCGCACCTCA
    GCGTTATCAT CCAGCTGCGG GACAGAGGCT CCGTCCCTCA CACAGCCCTC AGCTCCACAT
    GAAACCATTG TCCTCTCATT GGCCGACTGG GAAAGGAAAC AGGAAATGCT GGAGAAACAG
    GCACAGACGG GCCAGAGTAA CGGATTGACA CACAGTCCTG ATCCAGTAAT TGCAGCAGCT
    CAGCAGAACG GACAAAGAGC TGAAGCTCAG GAGAGCCAGA TCACAGCTAC ACCACAACTG
    CAGTTCATAC AGGATGGCTC GTGGAGCTGC AAGTCTAAAG GCAGGCAGGA GGATCTGAAG
    AAAACCGCCT CGCTGGATCG TAAACAGAGT CCAGCCCCAA GCCAGACCAC AGGGATGAGG
    AGGACTGGGT CATGTGAGAA TGTTTTAGGA ACACACCCAT CAAAATCACC CACACCATCA
    CAAGACACGC CACCTCCATC ACCCAGCAGG TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT
    TGCGCTCATC CACTGGGTAA AGGAGCAGCA ATGATCATTG AAACCTTGGG GCTTTACTTC
    CACATCCAGT GTTTTAAGTG TGGCGTATGT AAAGGATTAC TTGGAGACAC AAGTGCTGGA
    ACTGACGTCA GGATTCGAAA TGGACTTCTT AATTGTCACG AGTGCTACAT AAAATCAAGG
    GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330959.1
    CDS353..3559
    Translation

    Target ORF information:

    RefSeq Version XM_021475284.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGATTC AGAGCTACTA GTGTTTCTGA TGAAGCGGAG
    AGCGTGAGTT TGATTGACAT GCGTGATGAA GAGGATGAGC TTGCATACCT TCGGCCGCAC
    AGCCAATCAC GACACGAGCA CCTACACAAC CAGTACAACA AAGTGAAGGA GGAGGAGGAC
    CAGTGGCAGG ACGATTTGGC TCGTTGGAAG AACCGGAGGC GAAGCGCTTC TCAAGATCTG
    ATCAAAAAGG AGGAGGAGAG GAAGATGATG GAGAAGCTAA TGACAACGGA TGGAGGACAC
    GGACATCGCA AGAAGAGCAT CAAAACATAC AAAGAAATTG TAGAGGAGAA AGAGAAAAGG
    GAACAGGAGC TGCATGAACA ATACTGTAAG GCCTCCAGTC CAGAAGAGAA AGCAGCTGTG
    CTTCAGCGAT ATGCTCTCCG TTTCACCATC AGCGATGCCA TACTGGAGAA ACTCCAGCTC
    CCCAAACTGC CTTCTGCCGC CTCACCGATG CACACGTCCC CCCTCCATCA GCCCGAGCAA
    AAGACCACAT CCACTACATT AGTAGAAGCA GAGGTTCCAG AACTTTCACA AACTACCCAA
    ACACAACAGA CTGTCCATGT AAAGCAGAAG ATTACACATG ATGAGGCACC GGCACAGCCT
    AAAATCCCCA AACCTACACC ACCTCCCAAG TGCATCAAAG CTCCAGAAGT CCTGTCGGCA
    CCTTCACGAG TTCCTCCAGC TCCATCAAAG CCTGTACCCC TGATCACCCC CAAACCCTAC
    AGCCAGCCCA AATTCAGCCA AGGCTTCCGC AAATCGTTGA AGAGTGATGG GCTGATACGT
    GTGAACGGAG ACCACAATCA GCAGGTGATA AGAGAGAAGG CTAATAGAGA TCCTCTGCAG
    AGCCCTGAAG TCAACTTCTC TTCCTCACCT CCAAAGCCAG AAATTCAAAG TCAACAGAAT
    GAGGATCCAA TCGTGTTAGA AACCACAGAC CACCAGCAAG ACCTACAGAC ACCTAAACCT
    GACCTGGAGA AAGAAGTCAT AAAGAAGGAG GAAGAAAGTC TGCTTAGTCC TCAGAAAGAG
    ATCAGCAAAG AAGACACCTC AACTACACTT TCAGTGTCAG AACCAGCAGG AAAGCAGCCA
    GTGCCTGACA CAGTGATGAA GCGCAACTTT GTTGTCACAA CAACCATAGT GACAGAACTC
    ACTCAGACGC ACCCCGTCCC ACTAGAGGAC ACGTCCAGCA CAGAGCAGCC TGATACAGTC
    CAAAATACAT GTGATCTGAG CTCAACTGCA GAGTCCTGTA AAGCTGATCA GTCTTCATAC
    TCGATCTCGT CGACAGAGGG GATCGACTAC TCCATTGAAT GTATTGAAAC CCCAATGTTG
    AACCTTGCAA AAAGGGTTGA CCACTGGACG TGGGATCCCA ATGAGGAGCG CAGGCGGCAG
    GAGAGGTGGC AGCAGGAGCA GGAGCGTCTC CTGCAGGAGA AATATCAGCG CGAGCAGGAG
    AAACTGAAGG AGGAATGGGA GAAAGCCCAG AGAGAAGTAG AAGAGGAAGA AAGGAGACAC
    CATGAAGAGG AGAGACGAAT TCTGGAGGAG ACAGTGACCC CGCTGACCCC TCGCACCTCA
    GCGTTATCAT CCAGCTGCGG GACAGAGGCT CCGTCCCTCA CACAGCCCTC AGCTCCACAT
    GAAACCATTG TCCTCTCATT GGCCGACTGG GAAAGGAAAC AGGAAATGCT GGAGAAACAG
    GCACAGACGG GCCAGAGTAA CGGATTGACA CACAGTCCTG ATCCAGTAAT TGCAGCAGCT
    CAGCAGAACG GACAAAGAGC TGAAGCTCAG GAGAGCCAGA TCACAGCTAC ACCACAACTG
    CAGTTCATAC AGGATGGCTC GTGGAGCTGC AAGTCTAAAG GCAGGCAGGA GGATCTGAAG
    AAAACCGCCT CGCTGGATCG TAAACAGAGT CCAGCCCCAA GCCAGACCAC AGGGATGAGG
    AGGACTGGGT CATGTGAGAA TGTTTTAGGA ACACACCCAT CAAAATCACC CACACCATCA
    CAAGACACGC CACCTCCATC ACCCAGCAGG TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT
    TGCGCTCATC CACTGGGTAA AGGAGCAGCA ATGATCATTG AAACCTTGGG GCTTTACTTC
    CACATCCAGT GTTTTAAGTG TGGCGTATGT AAAGGATTAC TTGGAGACAC AAGTGCTGGA
    ACTGACGTCA GGATTCGAAA TGGACTTCTT AATTGTCACG AGTGCTACAT AAAATCAAGG
    GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa00739
    Clone ID Related Accession (Same CDS sequence) NM_001145577.1
    Accession Version NM_001145577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX323984.7. On Mar 5, 2009 this sequence version replaced XM_685337.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGGAGC GTGAGTTTGA TTGACATGCG TGATGAAGAG
    GATGAGCTTG CATACCTTCG GCCGCACAGC CAATCACGAC ACGAGCACCT ACACAACCAG
    TACAACAAAG TGAAGGAGGA GGAGGACCAG TGGCAGGACG ATTTGGCTCG TTGGAAGAAC
    CGGAGGCGAA GCGCTTCTCA AGATCTGATC AAAAAGGAGG AGGAGAGGAA GATGATGGAG
    AAGCTAATGA CAACGGATGG AGGACACGGA CATCGCAAGA AGAGCATCAA AACATACAAA
    GAAATTGTAG AGGAGAAAGA GAAAAGGGAA CAGGAGCTGC ATGAACAATA CTGTAAGGCC
    TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT CAGCGATATG CTCTCCGTTT CACCATCAGC
    GATGCCATAC TGGAGAAACT CCAGCTCCCC AAACTGCCTT CTGCCGCCTC ACCGATGCAC
    ACGTCCCCCC TCCATCAGCC CGAGCAAAAG ACCACATCCA CTACATTAGT AGAAGCAGAG
    GTTCCAGAAC TTTCACAAAC TACCCAAACA CAACAGACTG TCCATGTAAA GCAGAAGATT
    ACACATGATG AGGCACCGGC ACAGCCTAAA ATCCCCAAAC CTACACCACC TCCCAAGTGC
    ATCAAAGCTC CAGAAGTCCT GTCGGCACCT TCACGAGTTC CTCCAGCTCC ATCAAAGCCT
    GTACCCCTGA TCACCCCCAA ACCCTACAGC CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA
    TCGTTGAAGA GTGATGGGCT GATACGTGTG AACGGAGACC ACAATCAGCA GGTGATAAGA
    GAGAAGGCTA ATAGAGATCC TCTGCAGAGC CCTGAAGTCA ACTTCTCTTC CTCACCTCCA
    AAGCCAGAAA TTCAAAGTCA ACAGAATGAG GATCCAATCG TGTTAGAAAC CACAGACCAC
    CAGCAAGACC TACAGACACC TAAACCTGAC CTGGAGAAAG AAGTCATAAA GAAGGAGGAA
    GAAAGTCTGC TTAGTCCTCA GAAAGAGATC AGCAAAGAAG ACACCTCAAC TACACTTTCA
    GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG CCTGACACAG TGATGAAGCG CAACTTTGTT
    GTCACAACAA CCATAGTGAC AGAACTCACT CAGACGCACC CCGTCCCACT AGAGGACACG
    TCCAGCACAG AGCAGATTGA AACCCCAATG TTGAACCTTG CAAAAAGGGT TGACCACTGG
    ACGTGGGATC CCAATGAGGA GCGCAGGCGG CAGGAGAGGT GGCAGCAGGA GCAGGAGCGT
    CTCCTGCAGG AGAAATATCA GCGCGAGCAG GAGAAACTGA AGGAGGAATG GGAGAAAGCC
    CAGAGAGAAG TAGAAGAGGA AGAAAGGAGA CACCATGAAG AGGAGAGACG AATTCTGGAG
    GAGACAGTGA CCCCGCTGAC CCCTCGCACC TCAGCGTTAT CATCCAGCTG CGGGACAGAG
    GCTCCGTCCC TCACACAGCC CTCAGCTCCA CATGAAACCA TTGTCCTCTC ATTGGCCGAC
    TGGGAAAGGA AACAGGAAAT GCTGGAGAAA CAGGCACAGA CGGGCCAGAG TAACGGATTG
    ACACACAGTC CTGATCCAGT AATTGCAGCA GCTCAGCAGA ACGGACAAAG AGCTGAAGCT
    CAGGAGAGCC AGATCACAGC TACACCACAA CTGCAGTTCA TACAGGATGG CTCGTGGAGC
    TGCAAGTCTA AAGGCAGGCA GGAGGATCTG AAGAAAACCG CCTCGCTGGA TCGTAAACAG
    AGTCCAGCCC CAAGCCAGAC CACAGGGATG AGGAGGACTG GGTCATGTGA GAATGTTTTA
    GGAACACACC CATCAAAATC ACCCACACCA TCACAAGACA CGCCACCTCC ATCACCCAGC
    AGGTCAGTCA GTGGAAAGAA GCTTTGCTCC AGTTGCGCTC ATCCACTGGG TAAAGGAGCA
    GCAATGATCA TTGAAACCTT GGGGCTTTAC TTCCACATCC AGTGTTTTAA GTGTGGCGTA
    TGTAAAGGAT TACTTGGAGA CACAAGTGCT GGAACTGACG TCAGGATTCG AAATGGACTT
    CTTAATTGTC ACGAGTGCTA CATAAAATCA AGGGCTGCTG GTCAGCCAAC AACGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139049.1
    CDS221..3280
    Translation

    Target ORF information:

    RefSeq Version NM_001145577.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGGAGC GTGAGTTTGA TTGACATGCG TGATGAAGAG
    GATGAGCTTG CATACCTTCG GCCGCACAGC CAATCACGAC ACGAGCACCT ACACAACCAG
    TACAACAAAG TGAAGGAGGA GGAGGACCAG TGGCAGGACG ATTTGGCTCG TTGGAAGAAC
    CGGAGGCGAA GCGCTTCTCA AGATCTGATC AAAAAGGAGG AGGAGAGGAA GATGATGGAG
    AAGCTAATGA CAACGGATGG AGGACACGGA CATCGCAAGA AGAGCATCAA AACATACAAA
    GAAATTGTAG AGGAGAAAGA GAAAAGGGAA CAGGAGCTGC ATGAACAATA CTGTAAGGCC
    TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT CAGCGATATG CTCTCCGTTT CACCATCAGC
    GATGCCATAC TGGAGAAACT CCAGCTCCCC AAACTGCCTT CTGCCGCCTC ACCGATGCAC
    ACGTCCCCCC TCCATCAGCC CGAGCAAAAG ACCACATCCA CTACATTAGT AGAAGCAGAG
    GTTCCAGAAC TTTCACAAAC TACCCAAACA CAACAGACTG TCCATGTAAA GCAGAAGATT
    ACACATGATG AGGCACCGGC ACAGCCTAAA ATCCCCAAAC CTACACCACC TCCCAAGTGC
    ATCAAAGCTC CAGAAGTCCT GTCGGCACCT TCACGAGTTC CTCCAGCTCC ATCAAAGCCT
    GTACCCCTGA TCACCCCCAA ACCCTACAGC CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA
    TCGTTGAAGA GTGATGGGCT GATACGTGTG AACGGAGACC ACAATCAGCA GGTGATAAGA
    GAGAAGGCTA ATAGAGATCC TCTGCAGAGC CCTGAAGTCA ACTTCTCTTC CTCACCTCCA
    AAGCCAGAAA TTCAAAGTCA ACAGAATGAG GATCCAATCG TGTTAGAAAC CACAGACCAC
    CAGCAAGACC TACAGACACC TAAACCTGAC CTGGAGAAAG AAGTCATAAA GAAGGAGGAA
    GAAAGTCTGC TTAGTCCTCA GAAAGAGATC AGCAAAGAAG ACACCTCAAC TACACTTTCA
    GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG CCTGACACAG TGATGAAGCG CAACTTTGTT
    GTCACAACAA CCATAGTGAC AGAACTCACT CAGACGCACC CCGTCCCACT AGAGGACACG
    TCCAGCACAG AGCAGATTGA AACCCCAATG TTGAACCTTG CAAAAAGGGT TGACCACTGG
    ACGTGGGATC CCAATGAGGA GCGCAGGCGG CAGGAGAGGT GGCAGCAGGA GCAGGAGCGT
    CTCCTGCAGG AGAAATATCA GCGCGAGCAG GAGAAACTGA AGGAGGAATG GGAGAAAGCC
    CAGAGAGAAG TAGAAGAGGA AGAAAGGAGA CACCATGAAG AGGAGAGACG AATTCTGGAG
    GAGACAGTGA CCCCGCTGAC CCCTCGCACC TCAGCGTTAT CATCCAGCTG CGGGACAGAG
    GCTCCGTCCC TCACACAGCC CTCAGCTCCA CATGAAACCA TTGTCCTCTC ATTGGCCGAC
    TGGGAAAGGA AACAGGAAAT GCTGGAGAAA CAGGCACAGA CGGGCCAGAG TAACGGATTG
    ACACACAGTC CTGATCCAGT AATTGCAGCA GCTCAGCAGA ACGGACAAAG AGCTGAAGCT
    CAGGAGAGCC AGATCACAGC TACACCACAA CTGCAGTTCA TACAGGATGG CTCGTGGAGC
    TGCAAGTCTA AAGGCAGGCA GGAGGATCTG AAGAAAACCG CCTCGCTGGA TCGTAAACAG
    AGTCCAGCCC CAAGCCAGAC CACAGGGATG AGGAGGACTG GGTCATGTGA GAATGTTTTA
    GGAACACACC CATCAAAATC ACCCACACCA TCACAAGACA CGCCACCTCC ATCACCCAGC
    AGGTCAGTCA GTGGAAAGAA GCTTTGCTCC AGTTGCGCTC ATCCACTGGG TAAAGGAGCA
    GCAATGATCA TTGAAACCTT GGGGCTTTAC TTCCACATCC AGTGTTTTAA GTGTGGCGTA
    TGTAAAGGAT TACTTGGAGA CACAAGTGCT GGAACTGACG TCAGGATTCG AAATGGACTT
    CTTAATTGTC ACGAGTGCTA CATAAAATCA AGGGCTGCTG GTCAGCCAAC AACGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05614
    Clone ID Related Accession (Same CDS sequence) XM_005168356.3 , XM_005168356.4
    Accession Version XM_005168356.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGCTTC ACCCTTAGCT CTGTGGCCCC GCCCCCAAGA
    GATGCTGAGG ACATGCCCAG GAAGTGGCCT CAGATGATGG ACAGGCAGGA GGCGGGCTCT
    GGGGGACAGG AAAGTCTTAT TAAACATGAG ACTATTACCA AAAGCCTGTC CCCTATGCCT
    GAGCAACCGT CCCGTCGGCC CCTTTGGCAG GAAGATGATG ATGATCTACC CCCAATATTC
    AGAGCTACTA GTGTTTCTGA TGAAGCGGAG AGCGTGAGTT TGATTGACAT GCGTGATGAA
    GAGGATGAGC TTGCATACCT TCGGCCGCAC AGCCAATCAC GACACGAGCA CCTACACAAC
    CAGTACAACA AAGTGAAGGA GGAGGAGGAC CAGTGGCAGG ACGATTTGGC TCGTTGGAAG
    AACCGGAGGC GAAGCGCTTC TCAAGATCTG ATCAAAAAGG AGGAGGAGAG GAAGATGATG
    GAGAAGCTAA TGACAACGGA TGGAGGACAC GGACATCGCA AGAAGAGCAT CAAAACATAC
    AAAGAAATTG TAGAGGAGAA AGAGAAAAGG GAACAGGAGC TGCATGAACA ATACTGTAAG
    GCCTCCAGTC CAGAAGAGAA AGCAGCTGTG CTTCAGCGAT ATGCTCTCCG TTTCACCATC
    AGCGATGCCA TACTGGAGAA ACTCCAGCTC CCCAAACTGC CTTCTGCCGC CTCACCGATG
    CACACGTCCC CCCTCCATCA GCCCGAGCAA AAGACCACAT CCACTACATT AGTAGAAGCA
    GAGGTTCCAG AACTTTCACA AACTACCCAA ACACAACAGA CTGTCCATGT AAAGCAGAAG
    ATTACACATG ATGAGGCACC GGCACAGCCT AAAATCCCCA AACCTACACC ACCTCCCAAG
    TGCATCAAAG CTCCAGAAGT CCTGTCGGCA CCTTCACGAG TTCCTCCAGC TCCATCAAAG
    CCTGTACCCC TGATCACCCC CAAACCCTAC AGCCAGCCCA AATTCAGCCA AGGCTTCCGC
    AAATCGTTGA AGAGTGATGG GCTGATACGT GTGAACGGAG ACCACAATCA GCAGGTGATA
    AGAGAGAAGG CTAATAGAGA TCCTCTGCAG AGCCCTGAAG TCAACTTCTC TTCCTCACCT
    CCAAAGCCAG AAATTCAAAG TCAACAGAAT GAGGATCCAA TCGTGTTAGA AACCACAGAC
    CACCAGCAAG ACCTACAGAC ACCTAAACCT GACCTGGAGA AAGAAGTCAT AAAGAAGGAG
    GAAGAAAGTC TGCTTAGTCC TCAGAAAGAG ATCAGCAAAG AAGACACCTC AACTACACTT
    TCAGTGTCAG AACCAGCAGG AAAGCAGCCA GTGCCTGACA CAGTGATGAA GCGCAACTTT
    GTTGTCACAA CAACCATAGT GACAGAACTC ACTCAGACGC ACCCCGTCCC ACTAGAGGAC
    ACGTCCAGCA CAGAGCAGCC TGATACAGTC CAAAATACAT GTGATCTGAG CTCAACTGCA
    GAGTCCTGTA AAGCTGATCA GTCTTCATAC TCGATCTCGT CGACAGAGGG GATCGACTAC
    TCCATTGAAT GTATTGAAAC CCCAATGTTG AACCTTGCAA AAAGGGTTGA CCACTGGACG
    TGGGATCCCA ATGAGGAGCG CAGGCGGCAG GAGAGGTGGC AGCAGGAGCA GGAGCGTCTC
    CTGCAGGAGA AATATCAGCG CGAGCAGGAG AAACTGAAGG AGGAATGGGA GAAAGCCCAG
    AGAGAAGTAG AAGAGGAAGA AAGGAGACAC CATGAAGAGG AGAGACGAAT TCTGGAGGAG
    ACAGTGACCC CGCTGACCCC TCGCACCTCA GCGTTATCAT CCAGCTGCGG GACAGAGGCT
    CCGTCCCTCA CACAGCCCTC AGCTCCACAT GAAACCATTG TCCTCTCATT GGCCGACTGG
    GAAAGGAAAC AGGAAATGCT GGAGAAACAG GCACAGACGG GCCAGAGTAA CGGATTGACA
    CACAGTCCTG ATCCAGTAAT TGCAGCAGCT CAGCAGAACG GACAAAGAGC TGAAGCTCAG
    GAGAGCCAGA TCACAGCTAC ACCACAACTG CAGTTCATAC AGGATGGCTC GTGGAGCTGC
    AAGTCTAAAG GCAGGCAGGA GGATCTGAAG AAAACCGCCT CGCTGGATCG TAAACAGAGT
    CCAGCCCCAA GCCAGACCAC AGGGATGAGG AGGACTGGGT CATGTGAGAA TGTTTTAGGA
    ACACACCCAT CAAAATCACC CACACCATCA CAAGACACGC CACCTCCATC ACCCAGCAGG
    TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT TGCGCTCATC CACTGGGTAA AGGAGCAGCA
    ATGATCATTG AAACCTTGGG GCTTTACTTC CACATCCAGT GTTTTAAGTG TGGCGTATGT
    AAAGGATTAC TTGGAGACAC AAGTGCTGGA ACTGACGTCA GGATTCGAAA TGGACTTCTT
    AATTGTCACG AGTGCTACAT AAAATCAAGG GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168413.1
    CDS368..3784
    Translation

    Target ORF information:

    RefSeq Version XM_005168356.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168356.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGCTTC ACCCTTAGCT CTGTGGCCCC GCCCCCAAGA
    GATGCTGAGG ACATGCCCAG GAAGTGGCCT CAGATGATGG ACAGGCAGGA GGCGGGCTCT
    GGGGGACAGG AAAGTCTTAT TAAACATGAG ACTATTACCA AAAGCCTGTC CCCTATGCCT
    GAGCAACCGT CCCGTCGGCC CCTTTGGCAG GAAGATGATG ATGATCTACC CCCAATATTC
    AGAGCTACTA GTGTTTCTGA TGAAGCGGAG AGCGTGAGTT TGATTGACAT GCGTGATGAA
    GAGGATGAGC TTGCATACCT TCGGCCGCAC AGCCAATCAC GACACGAGCA CCTACACAAC
    CAGTACAACA AAGTGAAGGA GGAGGAGGAC CAGTGGCAGG ACGATTTGGC TCGTTGGAAG
    AACCGGAGGC GAAGCGCTTC TCAAGATCTG ATCAAAAAGG AGGAGGAGAG GAAGATGATG
    GAGAAGCTAA TGACAACGGA TGGAGGACAC GGACATCGCA AGAAGAGCAT CAAAACATAC
    AAAGAAATTG TAGAGGAGAA AGAGAAAAGG GAACAGGAGC TGCATGAACA ATACTGTAAG
    GCCTCCAGTC CAGAAGAGAA AGCAGCTGTG CTTCAGCGAT ATGCTCTCCG TTTCACCATC
    AGCGATGCCA TACTGGAGAA ACTCCAGCTC CCCAAACTGC CTTCTGCCGC CTCACCGATG
    CACACGTCCC CCCTCCATCA GCCCGAGCAA AAGACCACAT CCACTACATT AGTAGAAGCA
    GAGGTTCCAG AACTTTCACA AACTACCCAA ACACAACAGA CTGTCCATGT AAAGCAGAAG
    ATTACACATG ATGAGGCACC GGCACAGCCT AAAATCCCCA AACCTACACC ACCTCCCAAG
    TGCATCAAAG CTCCAGAAGT CCTGTCGGCA CCTTCACGAG TTCCTCCAGC TCCATCAAAG
    CCTGTACCCC TGATCACCCC CAAACCCTAC AGCCAGCCCA AATTCAGCCA AGGCTTCCGC
    AAATCGTTGA AGAGTGATGG GCTGATACGT GTGAACGGAG ACCACAATCA GCAGGTGATA
    AGAGAGAAGG CTAATAGAGA TCCTCTGCAG AGCCCTGAAG TCAACTTCTC TTCCTCACCT
    CCAAAGCCAG AAATTCAAAG TCAACAGAAT GAGGATCCAA TCGTGTTAGA AACCACAGAC
    CACCAGCAAG ACCTACAGAC ACCTAAACCT GACCTGGAGA AAGAAGTCAT AAAGAAGGAG
    GAAGAAAGTC TGCTTAGTCC TCAGAAAGAG ATCAGCAAAG AAGACACCTC AACTACACTT
    TCAGTGTCAG AACCAGCAGG AAAGCAGCCA GTGCCTGACA CAGTGATGAA GCGCAACTTT
    GTTGTCACAA CAACCATAGT GACAGAACTC ACTCAGACGC ACCCCGTCCC ACTAGAGGAC
    ACGTCCAGCA CAGAGCAGCC TGATACAGTC CAAAATACAT GTGATCTGAG CTCAACTGCA
    GAGTCCTGTA AAGCTGATCA GTCTTCATAC TCGATCTCGT CGACAGAGGG GATCGACTAC
    TCCATTGAAT GTATTGAAAC CCCAATGTTG AACCTTGCAA AAAGGGTTGA CCACTGGACG
    TGGGATCCCA ATGAGGAGCG CAGGCGGCAG GAGAGGTGGC AGCAGGAGCA GGAGCGTCTC
    CTGCAGGAGA AATATCAGCG CGAGCAGGAG AAACTGAAGG AGGAATGGGA GAAAGCCCAG
    AGAGAAGTAG AAGAGGAAGA AAGGAGACAC CATGAAGAGG AGAGACGAAT TCTGGAGGAG
    ACAGTGACCC CGCTGACCCC TCGCACCTCA GCGTTATCAT CCAGCTGCGG GACAGAGGCT
    CCGTCCCTCA CACAGCCCTC AGCTCCACAT GAAACCATTG TCCTCTCATT GGCCGACTGG
    GAAAGGAAAC AGGAAATGCT GGAGAAACAG GCACAGACGG GCCAGAGTAA CGGATTGACA
    CACAGTCCTG ATCCAGTAAT TGCAGCAGCT CAGCAGAACG GACAAAGAGC TGAAGCTCAG
    GAGAGCCAGA TCACAGCTAC ACCACAACTG CAGTTCATAC AGGATGGCTC GTGGAGCTGC
    AAGTCTAAAG GCAGGCAGGA GGATCTGAAG AAAACCGCCT CGCTGGATCG TAAACAGAGT
    CCAGCCCCAA GCCAGACCAC AGGGATGAGG AGGACTGGGT CATGTGAGAA TGTTTTAGGA
    ACACACCCAT CAAAATCACC CACACCATCA CAAGACACGC CACCTCCATC ACCCAGCAGG
    TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT TGCGCTCATC CACTGGGTAA AGGAGCAGCA
    ATGATCATTG AAACCTTGGG GCTTTACTTC CACATCCAGT GTTTTAAGTG TGGCGTATGT
    AAAGGATTAC TTGGAGACAC AAGTGCTGGA ACTGACGTCA GGATTCGAAA TGGACTTCTT
    AATTGTCACG AGTGCTACAT AAAATCAAGG GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05614
    Clone ID Related Accession (Same CDS sequence) XM_005168356.3 , XM_005168356.4
    Accession Version XM_005168356.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168356.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGCTTC ACCCTTAGCT CTGTGGCCCC GCCCCCAAGA
    GATGCTGAGG ACATGCCCAG GAAGTGGCCT CAGATGATGG ACAGGCAGGA GGCGGGCTCT
    GGGGGACAGG AAAGTCTTAT TAAACATGAG ACTATTACCA AAAGCCTGTC CCCTATGCCT
    GAGCAACCGT CCCGTCGGCC CCTTTGGCAG GAAGATGATG ATGATCTACC CCCAATATTC
    AGAGCTACTA GTGTTTCTGA TGAAGCGGAG AGCGTGAGTT TGATTGACAT GCGTGATGAA
    GAGGATGAGC TTGCATACCT TCGGCCGCAC AGCCAATCAC GACACGAGCA CCTACACAAC
    CAGTACAACA AAGTGAAGGA GGAGGAGGAC CAGTGGCAGG ACGATTTGGC TCGTTGGAAG
    AACCGGAGGC GAAGCGCTTC TCAAGATCTG ATCAAAAAGG AGGAGGAGAG GAAGATGATG
    GAGAAGCTAA TGACAACGGA TGGAGGACAC GGACATCGCA AGAAGAGCAT CAAAACATAC
    AAAGAAATTG TAGAGGAGAA AGAGAAAAGG GAACAGGAGC TGCATGAACA ATACTGTAAG
    GCCTCCAGTC CAGAAGAGAA AGCAGCTGTG CTTCAGCGAT ATGCTCTCCG TTTCACCATC
    AGCGATGCCA TACTGGAGAA ACTCCAGCTC CCCAAACTGC CTTCTGCCGC CTCACCGATG
    CACACGTCCC CCCTCCATCA GCCCGAGCAA AAGACCACAT CCACTACATT AGTAGAAGCA
    GAGGTTCCAG AACTTTCACA AACTACCCAA ACACAACAGA CTGTCCATGT AAAGCAGAAG
    ATTACACATG ATGAGGCACC GGCACAGCCT AAAATCCCCA AACCTACACC ACCTCCCAAG
    TGCATCAAAG CTCCAGAAGT CCTGTCGGCA CCTTCACGAG TTCCTCCAGC TCCATCAAAG
    CCTGTACCCC TGATCACCCC CAAACCCTAC AGCCAGCCCA AATTCAGCCA AGGCTTCCGC
    AAATCGTTGA AGAGTGATGG GCTGATACGT GTGAACGGAG ACCACAATCA GCAGGTGATA
    AGAGAGAAGG CTAATAGAGA TCCTCTGCAG AGCCCTGAAG TCAACTTCTC TTCCTCACCT
    CCAAAGCCAG AAATTCAAAG TCAACAGAAT GAGGATCCAA TCGTGTTAGA AACCACAGAC
    CACCAGCAAG ACCTACAGAC ACCTAAACCT GACCTGGAGA AAGAAGTCAT AAAGAAGGAG
    GAAGAAAGTC TGCTTAGTCC TCAGAAAGAG ATCAGCAAAG AAGACACCTC AACTACACTT
    TCAGTGTCAG AACCAGCAGG AAAGCAGCCA GTGCCTGACA CAGTGATGAA GCGCAACTTT
    GTTGTCACAA CAACCATAGT GACAGAACTC ACTCAGACGC ACCCCGTCCC ACTAGAGGAC
    ACGTCCAGCA CAGAGCAGCC TGATACAGTC CAAAATACAT GTGATCTGAG CTCAACTGCA
    GAGTCCTGTA AAGCTGATCA GTCTTCATAC TCGATCTCGT CGACAGAGGG GATCGACTAC
    TCCATTGAAT GTATTGAAAC CCCAATGTTG AACCTTGCAA AAAGGGTTGA CCACTGGACG
    TGGGATCCCA ATGAGGAGCG CAGGCGGCAG GAGAGGTGGC AGCAGGAGCA GGAGCGTCTC
    CTGCAGGAGA AATATCAGCG CGAGCAGGAG AAACTGAAGG AGGAATGGGA GAAAGCCCAG
    AGAGAAGTAG AAGAGGAAGA AAGGAGACAC CATGAAGAGG AGAGACGAAT TCTGGAGGAG
    ACAGTGACCC CGCTGACCCC TCGCACCTCA GCGTTATCAT CCAGCTGCGG GACAGAGGCT
    CCGTCCCTCA CACAGCCCTC AGCTCCACAT GAAACCATTG TCCTCTCATT GGCCGACTGG
    GAAAGGAAAC AGGAAATGCT GGAGAAACAG GCACAGACGG GCCAGAGTAA CGGATTGACA
    CACAGTCCTG ATCCAGTAAT TGCAGCAGCT CAGCAGAACG GACAAAGAGC TGAAGCTCAG
    GAGAGCCAGA TCACAGCTAC ACCACAACTG CAGTTCATAC AGGATGGCTC GTGGAGCTGC
    AAGTCTAAAG GCAGGCAGGA GGATCTGAAG AAAACCGCCT CGCTGGATCG TAAACAGAGT
    CCAGCCCCAA GCCAGACCAC AGGGATGAGG AGGACTGGGT CATGTGAGAA TGTTTTAGGA
    ACACACCCAT CAAAATCACC CACACCATCA CAAGACACGC CACCTCCATC ACCCAGCAGG
    TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT TGCGCTCATC CACTGGGTAA AGGAGCAGCA
    ATGATCATTG AAACCTTGGG GCTTTACTTC CACATCCAGT GTTTTAAGTG TGGCGTATGT
    AAAGGATTAC TTGGAGACAC AAGTGCTGGA ACTGACGTCA GGATTCGAAA TGGACTTCTT
    AATTGTCACG AGTGCTACAT AAAATCAAGG GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168413.1
    CDS355..3771
    Translation

    Target ORF information:

    RefSeq Version XM_005168356.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168356.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGCTTC ACCCTTAGCT CTGTGGCCCC GCCCCCAAGA
    GATGCTGAGG ACATGCCCAG GAAGTGGCCT CAGATGATGG ACAGGCAGGA GGCGGGCTCT
    GGGGGACAGG AAAGTCTTAT TAAACATGAG ACTATTACCA AAAGCCTGTC CCCTATGCCT
    GAGCAACCGT CCCGTCGGCC CCTTTGGCAG GAAGATGATG ATGATCTACC CCCAATATTC
    AGAGCTACTA GTGTTTCTGA TGAAGCGGAG AGCGTGAGTT TGATTGACAT GCGTGATGAA
    GAGGATGAGC TTGCATACCT TCGGCCGCAC AGCCAATCAC GACACGAGCA CCTACACAAC
    CAGTACAACA AAGTGAAGGA GGAGGAGGAC CAGTGGCAGG ACGATTTGGC TCGTTGGAAG
    AACCGGAGGC GAAGCGCTTC TCAAGATCTG ATCAAAAAGG AGGAGGAGAG GAAGATGATG
    GAGAAGCTAA TGACAACGGA TGGAGGACAC GGACATCGCA AGAAGAGCAT CAAAACATAC
    AAAGAAATTG TAGAGGAGAA AGAGAAAAGG GAACAGGAGC TGCATGAACA ATACTGTAAG
    GCCTCCAGTC CAGAAGAGAA AGCAGCTGTG CTTCAGCGAT ATGCTCTCCG TTTCACCATC
    AGCGATGCCA TACTGGAGAA ACTCCAGCTC CCCAAACTGC CTTCTGCCGC CTCACCGATG
    CACACGTCCC CCCTCCATCA GCCCGAGCAA AAGACCACAT CCACTACATT AGTAGAAGCA
    GAGGTTCCAG AACTTTCACA AACTACCCAA ACACAACAGA CTGTCCATGT AAAGCAGAAG
    ATTACACATG ATGAGGCACC GGCACAGCCT AAAATCCCCA AACCTACACC ACCTCCCAAG
    TGCATCAAAG CTCCAGAAGT CCTGTCGGCA CCTTCACGAG TTCCTCCAGC TCCATCAAAG
    CCTGTACCCC TGATCACCCC CAAACCCTAC AGCCAGCCCA AATTCAGCCA AGGCTTCCGC
    AAATCGTTGA AGAGTGATGG GCTGATACGT GTGAACGGAG ACCACAATCA GCAGGTGATA
    AGAGAGAAGG CTAATAGAGA TCCTCTGCAG AGCCCTGAAG TCAACTTCTC TTCCTCACCT
    CCAAAGCCAG AAATTCAAAG TCAACAGAAT GAGGATCCAA TCGTGTTAGA AACCACAGAC
    CACCAGCAAG ACCTACAGAC ACCTAAACCT GACCTGGAGA AAGAAGTCAT AAAGAAGGAG
    GAAGAAAGTC TGCTTAGTCC TCAGAAAGAG ATCAGCAAAG AAGACACCTC AACTACACTT
    TCAGTGTCAG AACCAGCAGG AAAGCAGCCA GTGCCTGACA CAGTGATGAA GCGCAACTTT
    GTTGTCACAA CAACCATAGT GACAGAACTC ACTCAGACGC ACCCCGTCCC ACTAGAGGAC
    ACGTCCAGCA CAGAGCAGCC TGATACAGTC CAAAATACAT GTGATCTGAG CTCAACTGCA
    GAGTCCTGTA AAGCTGATCA GTCTTCATAC TCGATCTCGT CGACAGAGGG GATCGACTAC
    TCCATTGAAT GTATTGAAAC CCCAATGTTG AACCTTGCAA AAAGGGTTGA CCACTGGACG
    TGGGATCCCA ATGAGGAGCG CAGGCGGCAG GAGAGGTGGC AGCAGGAGCA GGAGCGTCTC
    CTGCAGGAGA AATATCAGCG CGAGCAGGAG AAACTGAAGG AGGAATGGGA GAAAGCCCAG
    AGAGAAGTAG AAGAGGAAGA AAGGAGACAC CATGAAGAGG AGAGACGAAT TCTGGAGGAG
    ACAGTGACCC CGCTGACCCC TCGCACCTCA GCGTTATCAT CCAGCTGCGG GACAGAGGCT
    CCGTCCCTCA CACAGCCCTC AGCTCCACAT GAAACCATTG TCCTCTCATT GGCCGACTGG
    GAAAGGAAAC AGGAAATGCT GGAGAAACAG GCACAGACGG GCCAGAGTAA CGGATTGACA
    CACAGTCCTG ATCCAGTAAT TGCAGCAGCT CAGCAGAACG GACAAAGAGC TGAAGCTCAG
    GAGAGCCAGA TCACAGCTAC ACCACAACTG CAGTTCATAC AGGATGGCTC GTGGAGCTGC
    AAGTCTAAAG GCAGGCAGGA GGATCTGAAG AAAACCGCCT CGCTGGATCG TAAACAGAGT
    CCAGCCCCAA GCCAGACCAC AGGGATGAGG AGGACTGGGT CATGTGAGAA TGTTTTAGGA
    ACACACCCAT CAAAATCACC CACACCATCA CAAGACACGC CACCTCCATC ACCCAGCAGG
    TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT TGCGCTCATC CACTGGGTAA AGGAGCAGCA
    ATGATCATTG AAACCTTGGG GCTTTACTTC CACATCCAGT GTTTTAAGTG TGGCGTATGT
    AAAGGATTAC TTGGAGACAC AAGTGCTGGA ACTGACGTCA GGATTCGAAA TGGACTTCTT
    AATTGTCACG AGTGCTACAT AAAATCAAGG GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05616
    Clone ID Related Accession (Same CDS sequence) XM_005168358.3 , XM_005168358.4
    Accession Version XM_005168358.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGGAGC GTGAGTTTGA TTGACATGCG TGATGAAGAG
    GATGAGCTTG CATACCTTCG GCCGCACAGC CAATCACGAC ACGAGCACCT ACACAACCAG
    TACAACAAAG TGAAGGAGGA GGAGGACCAG TGGCAGGACG ATTTGGCTCG TTGGAAGAAC
    CGGAGGCGAA GCGCTTCTCA AGATCTGATC AAAAAGGAGG AGGAGAGGAA GATGATGGAG
    AAGCTAATGA CAACGGATGG AGGACACGGA CATCGCAAGA AGAGCATCAA AACATACAAA
    GAAATTGTAG AGGAGAAAGA GAAAAGGGAA CAGGAGCTGC ATGAACAATA CTGTAAGGCC
    TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT CAGCGATATG CTCTCCGTTT CACCATCAGC
    GATGCCATAC TGGAGAAACT CCAGCTCCCC AAACTGCCTT CTGCCGCCTC ACCGATGCAC
    ACGTCCCCCC TCCATCAGCC CGAGCAAAAG ACCACATCCA CTACATTAGT AGAAGCAGAG
    GTTCCAGAAC TTTCACAAAC TACCCAAACA CAACAGACTG TCCATGTAAA GCAGAAGATT
    ACACATGATG AGGCACCGGC ACAGCCTAAA ATCCCCAAAC CTACACCACC TCCCAAGTGC
    ATCAAAGCTC CAGAAGTCCT GTCGGCACCT TCACGAGTTC CTCCAGCTCC ATCAAAGCCT
    GTACCCCTGA TCACCCCCAA ACCCTACAGC CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA
    TCGTTGAAGA GTGATGGGCT GATACGTGTG AACGGAGACC ACAATCAGCA GGTGATAAGA
    GAGAAGGCTA ATAGAGATCC TCTGCAGAGC CCTGAAGTCA ACTTCTCTTC CTCACCTCCA
    AAGCCAGAAA TTCAAAGTCA ACAGAATGAG GATCCAATCG TGTTAGAAAC CACAGACCAC
    CAGCAAGACC TACAGACACC TAAACCTGAC CTGGAGAAAG AAGTCATAAA GAAGGAGGAA
    GAAAGTCTGC TTAGTCCTCA GAAAGAGATC AGCAAAGAAG ACACCTCAAC TACACTTTCA
    GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG CCTGACACAG TGATGAAGCG CAACTTTGTT
    GTCACAACAA CCATAGTGAC AGAACTCACT CAGACGCACC CCGTCCCACT AGAGGACACG
    TCCAGCACAG AGCAGCCTGA TACAGTCCAA AATACATGTG ATCTGAGCTC AACTGCAGAG
    TCCTGTAAAG CTGATCAGTC TTCATACTCG ATCTCGTCGA CAGAGGGGAT CGACTACTCC
    ATTGAATGTA TTGAAACCCC AATGTTGAAC CTTGCAAAAA GGGTTGACCA CTGGACGTGG
    GATCCCAATG AGGAGCGCAG GCGGCAGGAG AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG
    CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA CTGAAGGAGG AATGGGAGAA AGCCCAGAGA
    GAAGTAGAAG AGGAAGAAAG GAGACACCAT GAAGAGGAGA GACGAATTCT GGAGGAGACA
    GTGACCCCGC TGACCCCTCG CACCTCAGCG TTATCATCCA GCTGCGGGAC AGAGGCTCCG
    TCCCTCACAC AGCCCTCAGC TCCACATGAA ACCATTGTCC TCTCATTGGC CGACTGGGAA
    AGGAAACAGG AAATGCTGGA GAAACAGGCA CAGACGGGCC AGAGTAACGG ATTGACACAC
    AGTCCTGATC CAGTAATTGC AGCAGCTCAG CAGAACGGAC AAAGAGCTGA AGCTCAGGAG
    AGCCAGATCA CAGCTACACC ACAACTGCAG TTCATACAGG ATGGCTCGTG GAGCTGCAAG
    TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA ACCGCCTCGC TGGATCGTAA ACAGAGTCCA
    GCCCCAAGCC AGACCACAGG GATGAGGAGG ACTGGGTCAT GTGAGAATGT TTTAGGAACA
    CACCCATCAA AATCACCCAC ACCATCACAA GACACGCCAC CTCCATCACC CAGCAGGTCA
    GTCAGTGGAA AGAAGCTTTG CTCCAGTTGC GCTCATCCAC TGGGTAAAGG AGCAGCAATG
    ATCATTGAAA CCTTGGGGCT TTACTTCCAC ATCCAGTGTT TTAAGTGTGG CGTATGTAAA
    GGATTACTTG GAGACACAAG TGCTGGAACT GACGTCAGGA TTCGAAATGG ACTTCTTAAT
    TGTCACGAGT GCTACATAAA ATCAAGGGCT GCTGGTCAGC CAACAACGTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168415.1
    CDS367..3540
    Translation

    Target ORF information:

    RefSeq Version XM_005168358.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168358.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGGAGC GTGAGTTTGA TTGACATGCG TGATGAAGAG
    GATGAGCTTG CATACCTTCG GCCGCACAGC CAATCACGAC ACGAGCACCT ACACAACCAG
    TACAACAAAG TGAAGGAGGA GGAGGACCAG TGGCAGGACG ATTTGGCTCG TTGGAAGAAC
    CGGAGGCGAA GCGCTTCTCA AGATCTGATC AAAAAGGAGG AGGAGAGGAA GATGATGGAG
    AAGCTAATGA CAACGGATGG AGGACACGGA CATCGCAAGA AGAGCATCAA AACATACAAA
    GAAATTGTAG AGGAGAAAGA GAAAAGGGAA CAGGAGCTGC ATGAACAATA CTGTAAGGCC
    TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT CAGCGATATG CTCTCCGTTT CACCATCAGC
    GATGCCATAC TGGAGAAACT CCAGCTCCCC AAACTGCCTT CTGCCGCCTC ACCGATGCAC
    ACGTCCCCCC TCCATCAGCC CGAGCAAAAG ACCACATCCA CTACATTAGT AGAAGCAGAG
    GTTCCAGAAC TTTCACAAAC TACCCAAACA CAACAGACTG TCCATGTAAA GCAGAAGATT
    ACACATGATG AGGCACCGGC ACAGCCTAAA ATCCCCAAAC CTACACCACC TCCCAAGTGC
    ATCAAAGCTC CAGAAGTCCT GTCGGCACCT TCACGAGTTC CTCCAGCTCC ATCAAAGCCT
    GTACCCCTGA TCACCCCCAA ACCCTACAGC CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA
    TCGTTGAAGA GTGATGGGCT GATACGTGTG AACGGAGACC ACAATCAGCA GGTGATAAGA
    GAGAAGGCTA ATAGAGATCC TCTGCAGAGC CCTGAAGTCA ACTTCTCTTC CTCACCTCCA
    AAGCCAGAAA TTCAAAGTCA ACAGAATGAG GATCCAATCG TGTTAGAAAC CACAGACCAC
    CAGCAAGACC TACAGACACC TAAACCTGAC CTGGAGAAAG AAGTCATAAA GAAGGAGGAA
    GAAAGTCTGC TTAGTCCTCA GAAAGAGATC AGCAAAGAAG ACACCTCAAC TACACTTTCA
    GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG CCTGACACAG TGATGAAGCG CAACTTTGTT
    GTCACAACAA CCATAGTGAC AGAACTCACT CAGACGCACC CCGTCCCACT AGAGGACACG
    TCCAGCACAG AGCAGCCTGA TACAGTCCAA AATACATGTG ATCTGAGCTC AACTGCAGAG
    TCCTGTAAAG CTGATCAGTC TTCATACTCG ATCTCGTCGA CAGAGGGGAT CGACTACTCC
    ATTGAATGTA TTGAAACCCC AATGTTGAAC CTTGCAAAAA GGGTTGACCA CTGGACGTGG
    GATCCCAATG AGGAGCGCAG GCGGCAGGAG AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG
    CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA CTGAAGGAGG AATGGGAGAA AGCCCAGAGA
    GAAGTAGAAG AGGAAGAAAG GAGACACCAT GAAGAGGAGA GACGAATTCT GGAGGAGACA
    GTGACCCCGC TGACCCCTCG CACCTCAGCG TTATCATCCA GCTGCGGGAC AGAGGCTCCG
    TCCCTCACAC AGCCCTCAGC TCCACATGAA ACCATTGTCC TCTCATTGGC CGACTGGGAA
    AGGAAACAGG AAATGCTGGA GAAACAGGCA CAGACGGGCC AGAGTAACGG ATTGACACAC
    AGTCCTGATC CAGTAATTGC AGCAGCTCAG CAGAACGGAC AAAGAGCTGA AGCTCAGGAG
    AGCCAGATCA CAGCTACACC ACAACTGCAG TTCATACAGG ATGGCTCGTG GAGCTGCAAG
    TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA ACCGCCTCGC TGGATCGTAA ACAGAGTCCA
    GCCCCAAGCC AGACCACAGG GATGAGGAGG ACTGGGTCAT GTGAGAATGT TTTAGGAACA
    CACCCATCAA AATCACCCAC ACCATCACAA GACACGCCAC CTCCATCACC CAGCAGGTCA
    GTCAGTGGAA AGAAGCTTTG CTCCAGTTGC GCTCATCCAC TGGGTAAAGG AGCAGCAATG
    ATCATTGAAA CCTTGGGGCT TTACTTCCAC ATCCAGTGTT TTAAGTGTGG CGTATGTAAA
    GGATTACTTG GAGACACAAG TGCTGGAACT GACGTCAGGA TTCGAAATGG ACTTCTTAAT
    TGTCACGAGT GCTACATAAA ATCAAGGGCT GCTGGTCAGC CAACAACGTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05616
    Clone ID Related Accession (Same CDS sequence) XM_005168358.3 , XM_005168358.4
    Accession Version XM_005168358.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168358.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGGAGC GTGAGTTTGA TTGACATGCG TGATGAAGAG
    GATGAGCTTG CATACCTTCG GCCGCACAGC CAATCACGAC ACGAGCACCT ACACAACCAG
    TACAACAAAG TGAAGGAGGA GGAGGACCAG TGGCAGGACG ATTTGGCTCG TTGGAAGAAC
    CGGAGGCGAA GCGCTTCTCA AGATCTGATC AAAAAGGAGG AGGAGAGGAA GATGATGGAG
    AAGCTAATGA CAACGGATGG AGGACACGGA CATCGCAAGA AGAGCATCAA AACATACAAA
    GAAATTGTAG AGGAGAAAGA GAAAAGGGAA CAGGAGCTGC ATGAACAATA CTGTAAGGCC
    TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT CAGCGATATG CTCTCCGTTT CACCATCAGC
    GATGCCATAC TGGAGAAACT CCAGCTCCCC AAACTGCCTT CTGCCGCCTC ACCGATGCAC
    ACGTCCCCCC TCCATCAGCC CGAGCAAAAG ACCACATCCA CTACATTAGT AGAAGCAGAG
    GTTCCAGAAC TTTCACAAAC TACCCAAACA CAACAGACTG TCCATGTAAA GCAGAAGATT
    ACACATGATG AGGCACCGGC ACAGCCTAAA ATCCCCAAAC CTACACCACC TCCCAAGTGC
    ATCAAAGCTC CAGAAGTCCT GTCGGCACCT TCACGAGTTC CTCCAGCTCC ATCAAAGCCT
    GTACCCCTGA TCACCCCCAA ACCCTACAGC CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA
    TCGTTGAAGA GTGATGGGCT GATACGTGTG AACGGAGACC ACAATCAGCA GGTGATAAGA
    GAGAAGGCTA ATAGAGATCC TCTGCAGAGC CCTGAAGTCA ACTTCTCTTC CTCACCTCCA
    AAGCCAGAAA TTCAAAGTCA ACAGAATGAG GATCCAATCG TGTTAGAAAC CACAGACCAC
    CAGCAAGACC TACAGACACC TAAACCTGAC CTGGAGAAAG AAGTCATAAA GAAGGAGGAA
    GAAAGTCTGC TTAGTCCTCA GAAAGAGATC AGCAAAGAAG ACACCTCAAC TACACTTTCA
    GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG CCTGACACAG TGATGAAGCG CAACTTTGTT
    GTCACAACAA CCATAGTGAC AGAACTCACT CAGACGCACC CCGTCCCACT AGAGGACACG
    TCCAGCACAG AGCAGCCTGA TACAGTCCAA AATACATGTG ATCTGAGCTC AACTGCAGAG
    TCCTGTAAAG CTGATCAGTC TTCATACTCG ATCTCGTCGA CAGAGGGGAT CGACTACTCC
    ATTGAATGTA TTGAAACCCC AATGTTGAAC CTTGCAAAAA GGGTTGACCA CTGGACGTGG
    GATCCCAATG AGGAGCGCAG GCGGCAGGAG AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG
    CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA CTGAAGGAGG AATGGGAGAA AGCCCAGAGA
    GAAGTAGAAG AGGAAGAAAG GAGACACCAT GAAGAGGAGA GACGAATTCT GGAGGAGACA
    GTGACCCCGC TGACCCCTCG CACCTCAGCG TTATCATCCA GCTGCGGGAC AGAGGCTCCG
    TCCCTCACAC AGCCCTCAGC TCCACATGAA ACCATTGTCC TCTCATTGGC CGACTGGGAA
    AGGAAACAGG AAATGCTGGA GAAACAGGCA CAGACGGGCC AGAGTAACGG ATTGACACAC
    AGTCCTGATC CAGTAATTGC AGCAGCTCAG CAGAACGGAC AAAGAGCTGA AGCTCAGGAG
    AGCCAGATCA CAGCTACACC ACAACTGCAG TTCATACAGG ATGGCTCGTG GAGCTGCAAG
    TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA ACCGCCTCGC TGGATCGTAA ACAGAGTCCA
    GCCCCAAGCC AGACCACAGG GATGAGGAGG ACTGGGTCAT GTGAGAATGT TTTAGGAACA
    CACCCATCAA AATCACCCAC ACCATCACAA GACACGCCAC CTCCATCACC CAGCAGGTCA
    GTCAGTGGAA AGAAGCTTTG CTCCAGTTGC GCTCATCCAC TGGGTAAAGG AGCAGCAATG
    ATCATTGAAA CCTTGGGGCT TTACTTCCAC ATCCAGTGTT TTAAGTGTGG CGTATGTAAA
    GGATTACTTG GAGACACAAG TGCTGGAACT GACGTCAGGA TTCGAAATGG ACTTCTTAAT
    TGTCACGAGT GCTACATAAA ATCAAGGGCT GCTGGTCAGC CAACAACGTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168415.1
    CDS353..3526
    Translation

    Target ORF information:

    RefSeq Version XM_005168358.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168358.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGGAGC GTGAGTTTGA TTGACATGCG TGATGAAGAG
    GATGAGCTTG CATACCTTCG GCCGCACAGC CAATCACGAC ACGAGCACCT ACACAACCAG
    TACAACAAAG TGAAGGAGGA GGAGGACCAG TGGCAGGACG ATTTGGCTCG TTGGAAGAAC
    CGGAGGCGAA GCGCTTCTCA AGATCTGATC AAAAAGGAGG AGGAGAGGAA GATGATGGAG
    AAGCTAATGA CAACGGATGG AGGACACGGA CATCGCAAGA AGAGCATCAA AACATACAAA
    GAAATTGTAG AGGAGAAAGA GAAAAGGGAA CAGGAGCTGC ATGAACAATA CTGTAAGGCC
    TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT CAGCGATATG CTCTCCGTTT CACCATCAGC
    GATGCCATAC TGGAGAAACT CCAGCTCCCC AAACTGCCTT CTGCCGCCTC ACCGATGCAC
    ACGTCCCCCC TCCATCAGCC CGAGCAAAAG ACCACATCCA CTACATTAGT AGAAGCAGAG
    GTTCCAGAAC TTTCACAAAC TACCCAAACA CAACAGACTG TCCATGTAAA GCAGAAGATT
    ACACATGATG AGGCACCGGC ACAGCCTAAA ATCCCCAAAC CTACACCACC TCCCAAGTGC
    ATCAAAGCTC CAGAAGTCCT GTCGGCACCT TCACGAGTTC CTCCAGCTCC ATCAAAGCCT
    GTACCCCTGA TCACCCCCAA ACCCTACAGC CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA
    TCGTTGAAGA GTGATGGGCT GATACGTGTG AACGGAGACC ACAATCAGCA GGTGATAAGA
    GAGAAGGCTA ATAGAGATCC TCTGCAGAGC CCTGAAGTCA ACTTCTCTTC CTCACCTCCA
    AAGCCAGAAA TTCAAAGTCA ACAGAATGAG GATCCAATCG TGTTAGAAAC CACAGACCAC
    CAGCAAGACC TACAGACACC TAAACCTGAC CTGGAGAAAG AAGTCATAAA GAAGGAGGAA
    GAAAGTCTGC TTAGTCCTCA GAAAGAGATC AGCAAAGAAG ACACCTCAAC TACACTTTCA
    GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG CCTGACACAG TGATGAAGCG CAACTTTGTT
    GTCACAACAA CCATAGTGAC AGAACTCACT CAGACGCACC CCGTCCCACT AGAGGACACG
    TCCAGCACAG AGCAGCCTGA TACAGTCCAA AATACATGTG ATCTGAGCTC AACTGCAGAG
    TCCTGTAAAG CTGATCAGTC TTCATACTCG ATCTCGTCGA CAGAGGGGAT CGACTACTCC
    ATTGAATGTA TTGAAACCCC AATGTTGAAC CTTGCAAAAA GGGTTGACCA CTGGACGTGG
    GATCCCAATG AGGAGCGCAG GCGGCAGGAG AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG
    CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA CTGAAGGAGG AATGGGAGAA AGCCCAGAGA
    GAAGTAGAAG AGGAAGAAAG GAGACACCAT GAAGAGGAGA GACGAATTCT GGAGGAGACA
    GTGACCCCGC TGACCCCTCG CACCTCAGCG TTATCATCCA GCTGCGGGAC AGAGGCTCCG
    TCCCTCACAC AGCCCTCAGC TCCACATGAA ACCATTGTCC TCTCATTGGC CGACTGGGAA
    AGGAAACAGG AAATGCTGGA GAAACAGGCA CAGACGGGCC AGAGTAACGG ATTGACACAC
    AGTCCTGATC CAGTAATTGC AGCAGCTCAG CAGAACGGAC AAAGAGCTGA AGCTCAGGAG
    AGCCAGATCA CAGCTACACC ACAACTGCAG TTCATACAGG ATGGCTCGTG GAGCTGCAAG
    TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA ACCGCCTCGC TGGATCGTAA ACAGAGTCCA
    GCCCCAAGCC AGACCACAGG GATGAGGAGG ACTGGGTCAT GTGAGAATGT TTTAGGAACA
    CACCCATCAA AATCACCCAC ACCATCACAA GACACGCCAC CTCCATCACC CAGCAGGTCA
    GTCAGTGGAA AGAAGCTTTG CTCCAGTTGC GCTCATCCAC TGGGTAAAGG AGCAGCAATG
    ATCATTGAAA CCTTGGGGCT TTACTTCCAC ATCCAGTGTT TTAAGTGTGG CGTATGTAAA
    GGATTACTTG GAGACACAAG TGCTGGAACT GACGTCAGGA TTCGAAATGG ACTTCTTAAT
    TGTCACGAGT GCTACATAAA ATCAAGGGCT GCTGGTCAGC CAACAACGTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05611
    Clone ID Related Accession (Same CDS sequence) XM_021475267.1
    Accession Version XM_021475267.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGCGA TGAGCCTATT GTCCCGCTAT GTCAGACGTG TCTTGAGAAA
    GGCTGCCTCT CTGTCGCCTC CAAGACGAAG TTTGCCAAGA ATGATCCGGC TGGTTGCTGG
    GATCATTCCT CCGGTGAACG CCGGCGTTTT GTGACATTTG GTGGTGTGAC AGAGATGGAG
    AGCACTTCCT GGAAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGATGGGGA GGATGAGGAA
    AAGGGAGAGA GAGAGGGGAA TGAGGGAGAG AAGCTTCGAT ATCTCCTGTC TGTGGCACCT
    GTTGCTGTGC CCACTATAGG GTTGAGCTCC AGACTGACAA AGAGAGCGGT GGGCAGTGGA
    GACGTAAACC CTGAGCCTGC TCCTATCATG CCTCCTGAAC CTCCAAACCC GTTCATGTTT
    GACCTTAAAC GGCCTTTGAC CTCCACCGAG CGCAGGGTGC TGGAGGATGA ACTGGTTGAG
    AAAGCAAAGG ATGAGAAGGA TGATGATGAA GAAGACGAAG CCGAAGAAAG GCAGCCGGAT
    ATTGAAAAGG ATGATATGCT AGCTCGCAGA ACAAGAGCAT TTAAGAAGAC AGCGGGTGGG
    ACTTCGTTTA ACCAGTTCCT CCCGCTGCCG GGGTCCAAGA GAGAGGGAAC TGTAGGTGGT
    TCTTTAGCCC AGAAAGGAGG TGTACTGATA GGCTCGAGTG GAGACAGAGA CCTGCACTAC
    CTGGAGAGAA ATATAAGGAC TAAGAGAACC AAGATTGGAC AGAGATTTGA ACCAGTCGAT
    CAAGTAGCAA AGACACCGGT TGCTGTAGTC ACCCAGCAGA GCCCTGATAT TGTGCGCTCT
    ACTGCATCTA ACACTGATCG CTCTGCAAGT TGCGATTATG ATGATGATGA TGATGATGAA
    TATGTGGATG ACGGTCAATC TCCATATGTT GAGAAGGATG ATATGCTTGC GCGAAGAACT
    GGATCCTATC AAAAGCCAAA CGCAGGGGCA GGACAGTCTT TTAATGCATT TCTGCCCAAA
    CCTGGTGCTG TCAAACATAA AATCACCCCT GTCAGCGGAT CACAGTCTTG CGTTAAGCCA
    AGAGAGCATG AGACAGCCCC ATCACAGGAC AGCTTCACCC TTAGCTCTGT GGCCCCGCCC
    CCAAGAGATG CTGAGGACAT GCCCAGGAAG TGGCCTCAGA TGATGGACAG GCAGGAGGCG
    GGCTCTGGGG GACAGGAAAG TCTTATTAAA CATGAGACTA TTACCAAAAG CCTGTCCCCT
    ATGCCTGAGC AACCGTCCCG TCGGCCCCTT TGGCAGGAAG ATGATGATGA TCTACCCCCA
    ATATTCAGAG CTACTAGTGT TTCTGATGAA GCGGAGAGCG TGAGTTTGAT TGACATGCGT
    GATGAAGAGG ATGAGCTTGC ATACCTTCGG CCGCACAGCC AATCACGACA CGAGCACCTA
    CACAACCAGT ACAACAAAGT GAAGGAGGAG GAGGACCAGT GGCAGGACGA TTTGGCTCGT
    TGGAAGAACC GGAGGCGAAG CGCTTCTCAA GATCTGATCA AAAAGGAGGA GGAGAGGAAG
    ATGATGGAGA AGCTAATGAC AACGGATGGA GGACACGGAC ATCGCAAGAA GAGCATCAAA
    ACATACAAAG AAATTGTAGA GGAGAAAGAG AAAAGGGAAC AGGAGCTGCA TGAACAATAC
    TGTAAGGCCT CCAGTCCAGA AGAGAAAGCA GCTGTGCTTC AGCGATATGC TCTCCGTTTC
    ACCATCAGCG ATGCCATACT GGAGAAACTC CAGCTCCCCA AACTGCCTTC TGCCGCCTCA
    CCGATGCACA CGTCCCCCCT CCATCAGCCC GAGCAAAAGA CCACATCCAC TACATTAGTA
    GAAGCAGAGG TTCCAGAACT TTCACAAACT ACCCAAACAC AACAGACTGT CCATGTAAAG
    CAGAAGATTA CACATGATGA GGCACCGGCA CAGCCTAAAA TCCCCAAACC TACACCACCT
    CCCAAGTGCA TCAAAGCTCC AGAAGTCCTG TCGGCACCTT CACGAGTTCC TCCAGCTCCA
    TCAAAGCCTG TACCCCTGAT CACCCCCAAA CCCTACAGCC AGCCCAAATT CAGCCAAGGC
    TTCCGCAAAT CGTTGAAGAG TGATGGGCTG ATACGTGTGA ACGGAGACCA CAATCAGCAG
    GTGATAAGAG AGAAGGCTAA TAGAGATCCT CTGCAGAGCC CTGAAGTCAA CTTCTCTTCC
    TCACCTCCAA AGCCAGAAAT TCAAAGTCAA CAGAATGAGG ATCCAATCGT GTTAGAAACC
    ACAGACCACC AGCAAGACCT ACAGACACCT AAACCTGACC TGGAGAAAGA AGTCATAAAG
    AAGGAGGAAG AAAGTCTGCT TAGTCCTCAG AAAGAGATCA GCAAAGAAGA CACCTCAACT
    ACACTTTCAG TGTCAGAACC AGCAGGAAAG CAGCCAGTGC CTGACACAGT GATGAAGCGC
    AACTTTGTTG TCACAACAAC CATAGTGACA GAACTCACTC AGACGCACCC CGTCCCACTA
    GAGGACACGT CCAGCACAGA GCAGCCTGAT ACAGTCCAAA ATACATGTGA TCTGAGCTCA
    ACTGCAGAGT CCTGTAAAGC TGATCAGTCT TCATACTCGA TCTCGTCGAC AGAGGGGATC
    GACTACTCCA TTGAATGTAT TGAAACCCCA ATGTTGAACC TTGCAAAAAG GGTTGACCAC
    TGGACGTGGG ATCCCAATGA GGAGCGCAGG CGGCAGGAGA GGTGGCAGCA GGAGCAGGAG
    CGTCTCCTGC AGGAGAAATA TCAGCGCGAG CAGGAGAAAC TGAAGGAGGA ATGGGAGAAA
    GCCCAGAGAG AAGTAGAAGA GGAAGAAAGG AGACACCATG AAGAGGAGAG ACGAATTCTG
    GAGGAGACAG TGACCCCGCT GACCCCTCGC ACCTCAGCGT TATCATCCAG CTGCGGGACA
    GAGGCTCCGT CCCTCACACA GCCCTCAGCT CCACATGAAA CCATTGTCCT CTCATTGGCC
    GACTGGGAAA GGAAACAGGA AATGCTGGAG AAACAGGCAC AGACGGGCCA GAGTAACGGA
    TTGACACACA GTCCTGATCC AGTAATTGCA GCAGCTCAGC AGAACGGACA AAGAGCTGAA
    GCTCAGGAGA GCCAGATCAC AGCTACACCA CAACTGCAGT TCATACAGGA TGGCTCGTGG
    AGCTGCAAGT CTAAAGGCAG GCAGGAGGAT CTGAAGAAAA CCGCCTCGCT GGATCGTAAA
    CAGAGTCCAG CCCCAAGCCA GACCACAGGG ATGAGGAGGA CTGGGTCATG TGAGAATGTT
    TTAGGAACAC ACCCATCAAA ATCACCCACA CCATCACAAG ACACGCCACC TCCATCACCC
    AGCAGGTCAG TCAGTGGAAA GAAGCTTTGC TCCAGTTGCG CTCATCCACT GGGTAAAGGA
    GCAGCAATGA TCATTGAAAC CTTGGGGCTT TACTTCCACA TCCAGTGTTT TAAGTGTGGC
    GTATGTAAAG GATTACTTGG AGACACAAGT GCTGGAACTG ACGTCAGGAT TCGAAATGGA
    CTTCTTAATT GTCACGAGTG CTACATAAAA TCAAGGGCTG CTGGTCAGCC AACAACGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330942.1
    CDS370..6252
    Translation

    Target ORF information:

    RefSeq Version XM_021475267.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475267.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGCGA TGAGCCTATT GTCCCGCTAT GTCAGACGTG TCTTGAGAAA
    GGCTGCCTCT CTGTCGCCTC CAAGACGAAG TTTGCCAAGA ATGATCCGGC TGGTTGCTGG
    GATCATTCCT CCGGTGAACG CCGGCGTTTT GTGACATTTG GTGGTGTGAC AGAGATGGAG
    AGCACTTCCT GGAAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGATGGGGA GGATGAGGAA
    AAGGGAGAGA GAGAGGGGAA TGAGGGAGAG AAGCTTCGAT ATCTCCTGTC TGTGGCACCT
    GTTGCTGTGC CCACTATAGG GTTGAGCTCC AGACTGACAA AGAGAGCGGT GGGCAGTGGA
    GACGTAAACC CTGAGCCTGC TCCTATCATG CCTCCTGAAC CTCCAAACCC GTTCATGTTT
    GACCTTAAAC GGCCTTTGAC CTCCACCGAG CGCAGGGTGC TGGAGGATGA ACTGGTTGAG
    AAAGCAAAGG ATGAGAAGGA TGATGATGAA GAAGACGAAG CCGAAGAAAG GCAGCCGGAT
    ATTGAAAAGG ATGATATGCT AGCTCGCAGA ACAAGAGCAT TTAAGAAGAC AGCGGGTGGG
    ACTTCGTTTA ACCAGTTCCT CCCGCTGCCG GGGTCCAAGA GAGAGGGAAC TGTAGGTGGT
    TCTTTAGCCC AGAAAGGAGG TGTACTGATA GGCTCGAGTG GAGACAGAGA CCTGCACTAC
    CTGGAGAGAA ATATAAGGAC TAAGAGAACC AAGATTGGAC AGAGATTTGA ACCAGTCGAT
    CAAGTAGCAA AGACACCGGT TGCTGTAGTC ACCCAGCAGA GCCCTGATAT TGTGCGCTCT
    ACTGCATCTA ACACTGATCG CTCTGCAAGT TGCGATTATG ATGATGATGA TGATGATGAA
    TATGTGGATG ACGGTCAATC TCCATATGTT GAGAAGGATG ATATGCTTGC GCGAAGAACT
    GGATCCTATC AAAAGCCAAA CGCAGGGGCA GGACAGTCTT TTAATGCATT TCTGCCCAAA
    CCTGGTGCTG TCAAACATAA AATCACCCCT GTCAGCGGAT CACAGTCTTG CGTTAAGCCA
    AGAGAGCATG AGACAGCCCC ATCACAGGAC AGCTTCACCC TTAGCTCTGT GGCCCCGCCC
    CCAAGAGATG CTGAGGACAT GCCCAGGAAG TGGCCTCAGA TGATGGACAG GCAGGAGGCG
    GGCTCTGGGG GACAGGAAAG TCTTATTAAA CATGAGACTA TTACCAAAAG CCTGTCCCCT
    ATGCCTGAGC AACCGTCCCG TCGGCCCCTT TGGCAGGAAG ATGATGATGA TCTACCCCCA
    ATATTCAGAG CTACTAGTGT TTCTGATGAA GCGGAGAGCG TGAGTTTGAT TGACATGCGT
    GATGAAGAGG ATGAGCTTGC ATACCTTCGG CCGCACAGCC AATCACGACA CGAGCACCTA
    CACAACCAGT ACAACAAAGT GAAGGAGGAG GAGGACCAGT GGCAGGACGA TTTGGCTCGT
    TGGAAGAACC GGAGGCGAAG CGCTTCTCAA GATCTGATCA AAAAGGAGGA GGAGAGGAAG
    ATGATGGAGA AGCTAATGAC AACGGATGGA GGACACGGAC ATCGCAAGAA GAGCATCAAA
    ACATACAAAG AAATTGTAGA GGAGAAAGAG AAAAGGGAAC AGGAGCTGCA TGAACAATAC
    TGTAAGGCCT CCAGTCCAGA AGAGAAAGCA GCTGTGCTTC AGCGATATGC TCTCCGTTTC
    ACCATCAGCG ATGCCATACT GGAGAAACTC CAGCTCCCCA AACTGCCTTC TGCCGCCTCA
    CCGATGCACA CGTCCCCCCT CCATCAGCCC GAGCAAAAGA CCACATCCAC TACATTAGTA
    GAAGCAGAGG TTCCAGAACT TTCACAAACT ACCCAAACAC AACAGACTGT CCATGTAAAG
    CAGAAGATTA CACATGATGA GGCACCGGCA CAGCCTAAAA TCCCCAAACC TACACCACCT
    CCCAAGTGCA TCAAAGCTCC AGAAGTCCTG TCGGCACCTT CACGAGTTCC TCCAGCTCCA
    TCAAAGCCTG TACCCCTGAT CACCCCCAAA CCCTACAGCC AGCCCAAATT CAGCCAAGGC
    TTCCGCAAAT CGTTGAAGAG TGATGGGCTG ATACGTGTGA ACGGAGACCA CAATCAGCAG
    GTGATAAGAG AGAAGGCTAA TAGAGATCCT CTGCAGAGCC CTGAAGTCAA CTTCTCTTCC
    TCACCTCCAA AGCCAGAAAT TCAAAGTCAA CAGAATGAGG ATCCAATCGT GTTAGAAACC
    ACAGACCACC AGCAAGACCT ACAGACACCT AAACCTGACC TGGAGAAAGA AGTCATAAAG
    AAGGAGGAAG AAAGTCTGCT TAGTCCTCAG AAAGAGATCA GCAAAGAAGA CACCTCAACT
    ACACTTTCAG TGTCAGAACC AGCAGGAAAG CAGCCAGTGC CTGACACAGT GATGAAGCGC
    AACTTTGTTG TCACAACAAC CATAGTGACA GAACTCACTC AGACGCACCC CGTCCCACTA
    GAGGACACGT CCAGCACAGA GCAGCCTGAT ACAGTCCAAA ATACATGTGA TCTGAGCTCA
    ACTGCAGAGT CCTGTAAAGC TGATCAGTCT TCATACTCGA TCTCGTCGAC AGAGGGGATC
    GACTACTCCA TTGAATGTAT TGAAACCCCA ATGTTGAACC TTGCAAAAAG GGTTGACCAC
    TGGACGTGGG ATCCCAATGA GGAGCGCAGG CGGCAGGAGA GGTGGCAGCA GGAGCAGGAG
    CGTCTCCTGC AGGAGAAATA TCAGCGCGAG CAGGAGAAAC TGAAGGAGGA ATGGGAGAAA
    GCCCAGAGAG AAGTAGAAGA GGAAGAAAGG AGACACCATG AAGAGGAGAG ACGAATTCTG
    GAGGAGACAG TGACCCCGCT GACCCCTCGC ACCTCAGCGT TATCATCCAG CTGCGGGACA
    GAGGCTCCGT CCCTCACACA GCCCTCAGCT CCACATGAAA CCATTGTCCT CTCATTGGCC
    GACTGGGAAA GGAAACAGGA AATGCTGGAG AAACAGGCAC AGACGGGCCA GAGTAACGGA
    TTGACACACA GTCCTGATCC AGTAATTGCA GCAGCTCAGC AGAACGGACA AAGAGCTGAA
    GCTCAGGAGA GCCAGATCAC AGCTACACCA CAACTGCAGT TCATACAGGA TGGCTCGTGG
    AGCTGCAAGT CTAAAGGCAG GCAGGAGGAT CTGAAGAAAA CCGCCTCGCT GGATCGTAAA
    CAGAGTCCAG CCCCAAGCCA GACCACAGGG ATGAGGAGGA CTGGGTCATG TGAGAATGTT
    TTAGGAACAC ACCCATCAAA ATCACCCACA CCATCACAAG ACACGCCACC TCCATCACCC
    AGCAGGTCAG TCAGTGGAAA GAAGCTTTGC TCCAGTTGCG CTCATCCACT GGGTAAAGGA
    GCAGCAATGA TCATTGAAAC CTTGGGGCTT TACTTCCACA TCCAGTGTTT TAAGTGTGGC
    GTATGTAAAG GATTACTTGG AGACACAAGT GCTGGAACTG ACGTCAGGAT TCGAAATGGA
    CTTCTTAATT GTCACGAGTG CTACATAAAA TCAAGGGCTG CTGGTCAGCC AACAACGTTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147259
    Clone ID Related Accession (Same CDS sequence) XM_021475270.1
    Accession Version XM_021475270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGTAACCAT
    GGCCACTCTC TCAGCTTCAC CCTTAGCTCT GTGGCCCCGC CCCCAAGAGA TGCTGAGGAC
    ATGCCCAGGA AGTGGCCTCA GATGATGGAC AGGCAGGAGG CGGGCTCTGG GGGACAGGAA
    AGTCTTATTA AACATGAGAC TATTACCAAA AGCCTGTCCC CTATGCCTGA GCAACCGTCC
    CGTCGGCCCC TTTGGCAGGA AGATGATGAT GATCTACCCC CAATATTCAG AGCTACTAGT
    GTTTCTGATG AAGCGGAGAG CGTGAGTTTG ATTGACATGC GTGATGAAGA GGATGAGCTT
    GCATACCTTC GGCCGCACAG CCAATCACGA CACGAGCACC TACACAACCA GTACAACAAA
    GTGAAGGAGG AGGAGGACCA GTGGCAGGAC GATTTGGCTC GTTGGAAGAA CCGGAGGCGA
    AGCGCTTCTC AAGATCTGAT CAAAAAGGAG GAGGAGAGGA AGATGATGGA GAAGCTAATG
    ACAACGGATG GAGGACACGG ACATCGCAAG AAGAGCATCA AAACATACAA AGAAATTGTA
    GAGGAGAAAG AGAAAAGGGA ACAGGAGCTG CATGAACAAT ACTGTAAGGC CTCCAGTCCA
    GAAGAGAAAG CAGCTGTGCT TCAGCGATAT GCTCTCCGTT TCACCATCAG CGATGCCATA
    CTGGAGAAAC TCCAGCTCCC CAAACTGCCT TCTGCCGCCT CACCGATGCA CACGTCCCCC
    CTCCATCAGC CCGAGCAAAA GACCACATCC ACTACATTAG TAGAAGCAGA GGTTCCAGAA
    CTTTCACAAA CTACCCAAAC ACAACAGACT GTCCATGTAA AGCAGAAGAT TACACATGAT
    GAGGCACCGG CACAGCCTAA AATCCCCAAA CCTACACCAC CTCCCAAGTG CATCAAAGCT
    CCAGAAGTCC TGTCGGCACC TTCACGAGTT CCTCCAGCTC CATCAAAGCC TGTACCCCTG
    ATCACCCCCA AACCCTACAG CCAGCCCAAA TTCAGCCAAG GCTTCCGCAA ATCGTTGAAG
    AGTGATGGGC TGATACGTGT GAACGGAGAC CACAATCAGC AGGTGATAAG AGAGAAGGCT
    AATAGAGATC CTCTGCAGAG CCCTGAAGTC AACTTCTCTT CCTCACCTCC AAAGCCAGAA
    ATTCAAAGTC AACAGAATGA GGATCCAATC GTGTTAGAAA CCACAGACCA CCAGCAAGAC
    CTACAGACAC CTAAACCTGA CCTGGAGAAA GAAGTCATAA AGAAGGAGGA AGAAAGTCTG
    CTTAGTCCTC AGAAAGAGAT CAGCAAAGAA GACACCTCAA CTACACTTTC AGTGTCAGAA
    CCAGCAGGAA AGCAGCCAGT GCCTGACACA GTGATGAAGC GCAACTTTGT TGTCACAACA
    ACCATAGTGA CAGAACTCAC TCAGACGCAC CCCGTCCCAC TAGAGGACAC GTCCAGCACA
    GAGCAGCCTG ATACAGTCCA AAATACATGT GATCTGAGCT CAACTGCAGA GTCCTGTAAA
    GCTGATCAGT CTTCATACTC GATCTCGTCG ACAGAGGGGA TCGACTACTC CATTGAATGT
    ATTGAAACCC CAATGTTGAA CCTTGCAAAA AGGGTTGACC ACTGGACGTG GGATCCCAAT
    GAGGAGCGCA GGCGGCAGGA GAGGTGGCAG CAGGAGCAGG AGCGTCTCCT GCAGGAGAAA
    TATCAGCGCG AGCAGGAGAA ACTGAAGGAG GAATGGGAGA AAGCCCAGAG AGAAGTAGAA
    GAGGAAGAAA GGAGACACCA TGAAGAGGAG AGACGAATTC TGGAGGAGAC AGTGACCCCG
    CTGACCCCTC GCACCTCAGC GTTATCATCC AGCTGCGGGA CAGAGGCTCC GTCCCTCACA
    CAGCCCTCAG CTCCACATGA AACCATTGTC CTCTCATTGG CCGACTGGGA AAGGAAACAG
    GAAATGCTGG AGAAACAGGC ACAGACGGGC CAGAGTAACG GATTGACACA CAGTCCTGAT
    CCAGTAATTG CAGCAGCTCA GCAGAACGGA CAAAGAGCTG AAGCTCAGGA GAGCCAGATC
    ACAGCTACAC CACAACTGCA GTTCATACAG GATGGCTCGT GGAGCTGCAA GTCTAAAGGC
    AGGCAGGAGG ATCTGAAGAA AACCGCCTCG CTGGATCGTA AACAGAGTCC AGCCCCAAGC
    CAGACCACAG GGATGAGGAG GACTGGGTCA TGTGAGAATG TTTTAGGAAC ACACCCATCA
    AAATCACCCA CACCATCACA AGACACGCCA CCTCCATCAC CCAGCAGGTC AGTCAGTGGA
    AAGAAGCTTT GCTCCAGTTG CGCTCATCCA CTGGGTAAAG GAGCAGCAAT GATCATTGAA
    ACCTTGGGGC TTTACTTCCA CATCCAGTGT TTTAAGTGTG GCGTATGTAA AGGATTACTT
    GGAGACACAA GTGCTGGAAC TGACGTCAGG ATTCGAAATG GACTTCTTAA TTGTCACGAG
    TGCTACATAA AATCAAGGGC TGCTGGTCAG CCAACAACGT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330945.1
    CDS369..5933
    Translation

    Target ORF information:

    RefSeq Version XM_021475270.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGTAACCAT
    GGCCACTCTC TCAGCTTCAC CCTTAGCTCT GTGGCCCCGC CCCCAAGAGA TGCTGAGGAC
    ATGCCCAGGA AGTGGCCTCA GATGATGGAC AGGCAGGAGG CGGGCTCTGG GGGACAGGAA
    AGTCTTATTA AACATGAGAC TATTACCAAA AGCCTGTCCC CTATGCCTGA GCAACCGTCC
    CGTCGGCCCC TTTGGCAGGA AGATGATGAT GATCTACCCC CAATATTCAG AGCTACTAGT
    GTTTCTGATG AAGCGGAGAG CGTGAGTTTG ATTGACATGC GTGATGAAGA GGATGAGCTT
    GCATACCTTC GGCCGCACAG CCAATCACGA CACGAGCACC TACACAACCA GTACAACAAA
    GTGAAGGAGG AGGAGGACCA GTGGCAGGAC GATTTGGCTC GTTGGAAGAA CCGGAGGCGA
    AGCGCTTCTC AAGATCTGAT CAAAAAGGAG GAGGAGAGGA AGATGATGGA GAAGCTAATG
    ACAACGGATG GAGGACACGG ACATCGCAAG AAGAGCATCA AAACATACAA AGAAATTGTA
    GAGGAGAAAG AGAAAAGGGA ACAGGAGCTG CATGAACAAT ACTGTAAGGC CTCCAGTCCA
    GAAGAGAAAG CAGCTGTGCT TCAGCGATAT GCTCTCCGTT TCACCATCAG CGATGCCATA
    CTGGAGAAAC TCCAGCTCCC CAAACTGCCT TCTGCCGCCT CACCGATGCA CACGTCCCCC
    CTCCATCAGC CCGAGCAAAA GACCACATCC ACTACATTAG TAGAAGCAGA GGTTCCAGAA
    CTTTCACAAA CTACCCAAAC ACAACAGACT GTCCATGTAA AGCAGAAGAT TACACATGAT
    GAGGCACCGG CACAGCCTAA AATCCCCAAA CCTACACCAC CTCCCAAGTG CATCAAAGCT
    CCAGAAGTCC TGTCGGCACC TTCACGAGTT CCTCCAGCTC CATCAAAGCC TGTACCCCTG
    ATCACCCCCA AACCCTACAG CCAGCCCAAA TTCAGCCAAG GCTTCCGCAA ATCGTTGAAG
    AGTGATGGGC TGATACGTGT GAACGGAGAC CACAATCAGC AGGTGATAAG AGAGAAGGCT
    AATAGAGATC CTCTGCAGAG CCCTGAAGTC AACTTCTCTT CCTCACCTCC AAAGCCAGAA
    ATTCAAAGTC AACAGAATGA GGATCCAATC GTGTTAGAAA CCACAGACCA CCAGCAAGAC
    CTACAGACAC CTAAACCTGA CCTGGAGAAA GAAGTCATAA AGAAGGAGGA AGAAAGTCTG
    CTTAGTCCTC AGAAAGAGAT CAGCAAAGAA GACACCTCAA CTACACTTTC AGTGTCAGAA
    CCAGCAGGAA AGCAGCCAGT GCCTGACACA GTGATGAAGC GCAACTTTGT TGTCACAACA
    ACCATAGTGA CAGAACTCAC TCAGACGCAC CCCGTCCCAC TAGAGGACAC GTCCAGCACA
    GAGCAGCCTG ATACAGTCCA AAATACATGT GATCTGAGCT CAACTGCAGA GTCCTGTAAA
    GCTGATCAGT CTTCATACTC GATCTCGTCG ACAGAGGGGA TCGACTACTC CATTGAATGT
    ATTGAAACCC CAATGTTGAA CCTTGCAAAA AGGGTTGACC ACTGGACGTG GGATCCCAAT
    GAGGAGCGCA GGCGGCAGGA GAGGTGGCAG CAGGAGCAGG AGCGTCTCCT GCAGGAGAAA
    TATCAGCGCG AGCAGGAGAA ACTGAAGGAG GAATGGGAGA AAGCCCAGAG AGAAGTAGAA
    GAGGAAGAAA GGAGACACCA TGAAGAGGAG AGACGAATTC TGGAGGAGAC AGTGACCCCG
    CTGACCCCTC GCACCTCAGC GTTATCATCC AGCTGCGGGA CAGAGGCTCC GTCCCTCACA
    CAGCCCTCAG CTCCACATGA AACCATTGTC CTCTCATTGG CCGACTGGGA AAGGAAACAG
    GAAATGCTGG AGAAACAGGC ACAGACGGGC CAGAGTAACG GATTGACACA CAGTCCTGAT
    CCAGTAATTG CAGCAGCTCA GCAGAACGGA CAAAGAGCTG AAGCTCAGGA GAGCCAGATC
    ACAGCTACAC CACAACTGCA GTTCATACAG GATGGCTCGT GGAGCTGCAA GTCTAAAGGC
    AGGCAGGAGG ATCTGAAGAA AACCGCCTCG CTGGATCGTA AACAGAGTCC AGCCCCAAGC
    CAGACCACAG GGATGAGGAG GACTGGGTCA TGTGAGAATG TTTTAGGAAC ACACCCATCA
    AAATCACCCA CACCATCACA AGACACGCCA CCTCCATCAC CCAGCAGGTC AGTCAGTGGA
    AAGAAGCTTT GCTCCAGTTG CGCTCATCCA CTGGGTAAAG GAGCAGCAAT GATCATTGAA
    ACCTTGGGGC TTTACTTCCA CATCCAGTGT TTTAAGTGTG GCGTATGTAA AGGATTACTT
    GGAGACACAA GTGCTGGAAC TGACGTCAGG ATTCGAAATG GACTTCTTAA TTGTCACGAG
    TGCTACATAA AATCAAGGGC TGCTGGTCAG CCAACAACGT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147260
    Clone ID Related Accession (Same CDS sequence) XM_021475273.1
    Accession Version XM_021475273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGCGA TGAGCCTATT GTCCCGCTAT GTCAGACGTG TCTTGAGAAA
    GGCTGCCTCT CTGTCGCCTC CAAGACGAAG TTTGCCAAGA ATGATCCGGC TGGTTGCTGG
    GATCATTCCT CCGGTGAACG CCGGCGTTTT GTGACATTTG GTGGTGTGAC AGAGATGGAG
    AGCACTTCCT GGAAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGATGGGGA GGATGAGGAA
    AAGGGAGAGA GAGAGGGGAA TGAGGGAGAG AAGCTTCGAT ATCTCCTGTC TGTGGCACCT
    GTTGCTGTGC CCACTATAGG GTTGAGCTCC AGACTGACAA AGAGAGCGGT GGGCAGTGGA
    GACGTAAACC CTGAGCCTGC TCCTATCATG CCTCCTGAAC CTCCAAACCC GTTCATGTTT
    GACCTTAAAC GGCCTTTGAC CTCCACCGAG CGCAGGGTGC TGGAGGATGA ACTGGTTGAG
    AAAGCAAAGG ATGAGAAGGA TGATGATGAA GAAGACGAAG CCGAAGAAAG GCAGCCGGAT
    ATTGAAAAGG ATGATATGCT AGCTCGCAGA ACAAGAGCAT TTAAGAAGAC AGCGGGTGGG
    ACTTCGTTTA ACCAGTTCCT CCCGCTGCCG GGGTCCAAGA GAGAGGGAAC TGTAGGTGGT
    TCTTTAGCCC AGAAAGGAGG TGTACTGATA GGCTCGAGTG GAGACAGAGA CCTGCACTAC
    CTGGAGAGAA ATATAAGGAC TAAGAGAACC AAGATTGGAC AGAGCTTCAC CCTTAGCTCT
    GTGGCCCCGC CCCCAAGAGA TGCTGAGGAC ATGCCCAGGA AGTGGCCTCA GATGATGGAC
    AGGCAGGAGG CGGGCTCTGG GGGACAGGAA AGTCTTATTA AACATGAGAC TATTACCAAA
    AGCCTGTCCC CTATGCCTGA GCAACCGTCC CGTCGGCCCC TTTGGCAGGA AGATGATGAT
    GATCTACCCC CAATATTCAG AGCTACTAGT GTTTCTGATG AAGCGGAGAG CGTGAGTTTG
    ATTGACATGC GTGATGAAGA GGATGAGCTT GCATACCTTC GGCCGCACAG CCAATCACGA
    CACGAGCACC TACACAACCA GTACAACAAA GTGAAGGAGG AGGAGGACCA GTGGCAGGAC
    GATTTGGCTC GTTGGAAGAA CCGGAGGCGA AGCGCTTCTC AAGATCTGAT CAAAAAGGAG
    GAGGAGAGGA AGATGATGGA GAAGCTAATG ACAACGGATG GAGGACACGG ACATCGCAAG
    AAGAGCATCA AAACATACAA AGAAATTGTA GAGGAGAAAG AGAAAAGGGA ACAGGAGCTG
    CATGAACAAT ACTGTAAGGC CTCCAGTCCA GAAGAGAAAG CAGCTGTGCT TCAGCGATAT
    GCTCTCCGTT TCACCATCAG CGATGCCATA CTGGAGAAAC TCCAGCTCCC CAAACTGCCT
    TCTGCCGCCT CACCGATGCA CACGTCCCCC CTCCATCAGC CCGAGCAAAA GACCACATCC
    ACTACATTAG TAGAAGCAGA GGTTCCAGAA CTTTCACAAA CTACCCAAAC ACAACAGACT
    GTCCATGTAA AGCAGAAGAT TACACATGAT GAGGCACCGG CACAGCCTAA AATCCCCAAA
    CCTACACCAC CTCCCAAGTG CATCAAAGCT CCAGAAGTCC TGTCGGCACC TTCACGAGTT
    CCTCCAGCTC CATCAAAGCC TGTACCCCTG ATCACCCCCA AACCCTACAG CCAGCCCAAA
    TTCAGCCAAG GCTTCCGCAA ATCGTTGAAG AGTGATGGGC TGATACGTGT GAACGGAGAC
    CACAATCAGC AGGTGATAAG AGAGAAGGCT AATAGAGATC CTCTGCAGAG CCCTGAAGTC
    AACTTCTCTT CCTCACCTCC AAAGCCAGAA ATTCAAAGTC AACAGAATGA GGATCCAATC
    GTGTTAGAAA CCACAGACCA CCAGCAAGAC CTACAGACAC CTAAACCTGA CCTGGAGAAA
    GAAGTCATAA AGAAGGAGGA AGAAAGTCTG CTTAGTCCTC AGAAAGAGAT CAGCAAAGAA
    GACACCTCAA CTACACTTTC AGTGTCAGAA CCAGCAGGAA AGCAGCCAGT GCCTGACACA
    GTGATGAAGC GCAACTTTGT TGTCACAACA ACCATAGTGA CAGAACTCAC TCAGACGCAC
    CCCGTCCCAC TAGAGGACAC GTCCAGCACA GAGCAGCCTG ATACAGTCCA AAATACATGT
    GATCTGAGCT CAACTGCAGA GTCCTGTAAA GCTGATCAGT CTTCATACTC GATCTCGTCG
    ACAGAGGGGA TCGACTACTC CATTGAATGT ATTGAAACCC CAATGTTGAA CCTTGCAAAA
    AGGGTTGACC ACTGGACGTG GGATCCCAAT GAGGAGCGCA GGCGGCAGGA GAGGTGGCAG
    CAGGAGCAGG AGCGTCTCCT GCAGGAGAAA TATCAGCGCG AGCAGGAGAA ACTGAAGGAG
    GAATGGGAGA AAGCCCAGAG AGAAGTAGAA GAGGAAGAAA GGAGACACCA TGAAGAGGAG
    AGACGAATTC TGGAGGAGAC AGTGACCCCG CTGACCCCTC GCACCTCAGC GTTATCATCC
    AGCTGCGGGA CAGAGGCTCC GTCCCTCACA CAGCCCTCAG CTCCACATGA AACCATTGTC
    CTCTCATTGG CCGACTGGGA AAGGAAACAG GAAATGCTGG AGAAACAGGC ACAGACGGGC
    CAGAGTAACG GATTGACACA CAGTCCTGAT CCAGTAATTG CAGCAGCTCA GCAGAACGGA
    CAAAGAGCTG AAGCTCAGGA GAGCCAGATC ACAGCTACAC CACAACTGCA GTTCATACAG
    GATGGCTCGT GGAGCTGCAA GTCTAAAGGC AGGCAGGAGG ATCTGAAGAA AACCGCCTCG
    CTGGATCGTA AACAGAGTCC AGCCCCAAGC CAGACCACAG GGATGAGGAG GACTGGGTCA
    TGTGAGAATG TTTTAGGAAC ACACCCATCA AAATCACCCA CACCATCACA AGACACGCCA
    CCTCCATCAC CCAGCAGGTC AGTCAGTGGA AAGAAGCTTT GCTCCAGTTG CGCTCATCCA
    CTGGGTAAAG GAGCAGCAAT GATCATTGAA ACCTTGGGGC TTTACTTCCA CATCCAGTGT
    TTTAAGTGTG GCGTATGTAA AGGATTACTT GGAGACACAA GTGCTGGAAC TGACGTCAGG
    ATTCGAAATG GACTTCTTAA TTGTCACGAG TGCTACATAA AATCAAGGGC TGCTGGTCAG
    CCAACAACGT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330948.1
    CDS369..5903
    Translation

    Target ORF information:

    RefSeq Version XM_021475273.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGCGA TGAGCCTATT GTCCCGCTAT GTCAGACGTG TCTTGAGAAA
    GGCTGCCTCT CTGTCGCCTC CAAGACGAAG TTTGCCAAGA ATGATCCGGC TGGTTGCTGG
    GATCATTCCT CCGGTGAACG CCGGCGTTTT GTGACATTTG GTGGTGTGAC AGAGATGGAG
    AGCACTTCCT GGAAAGAGGA GGAGGAGGAG GAGGAGGAGG AGGATGGGGA GGATGAGGAA
    AAGGGAGAGA GAGAGGGGAA TGAGGGAGAG AAGCTTCGAT ATCTCCTGTC TGTGGCACCT
    GTTGCTGTGC CCACTATAGG GTTGAGCTCC AGACTGACAA AGAGAGCGGT GGGCAGTGGA
    GACGTAAACC CTGAGCCTGC TCCTATCATG CCTCCTGAAC CTCCAAACCC GTTCATGTTT
    GACCTTAAAC GGCCTTTGAC CTCCACCGAG CGCAGGGTGC TGGAGGATGA ACTGGTTGAG
    AAAGCAAAGG ATGAGAAGGA TGATGATGAA GAAGACGAAG CCGAAGAAAG GCAGCCGGAT
    ATTGAAAAGG ATGATATGCT AGCTCGCAGA ACAAGAGCAT TTAAGAAGAC AGCGGGTGGG
    ACTTCGTTTA ACCAGTTCCT CCCGCTGCCG GGGTCCAAGA GAGAGGGAAC TGTAGGTGGT
    TCTTTAGCCC AGAAAGGAGG TGTACTGATA GGCTCGAGTG GAGACAGAGA CCTGCACTAC
    CTGGAGAGAA ATATAAGGAC TAAGAGAACC AAGATTGGAC AGAGCTTCAC CCTTAGCTCT
    GTGGCCCCGC CCCCAAGAGA TGCTGAGGAC ATGCCCAGGA AGTGGCCTCA GATGATGGAC
    AGGCAGGAGG CGGGCTCTGG GGGACAGGAA AGTCTTATTA AACATGAGAC TATTACCAAA
    AGCCTGTCCC CTATGCCTGA GCAACCGTCC CGTCGGCCCC TTTGGCAGGA AGATGATGAT
    GATCTACCCC CAATATTCAG AGCTACTAGT GTTTCTGATG AAGCGGAGAG CGTGAGTTTG
    ATTGACATGC GTGATGAAGA GGATGAGCTT GCATACCTTC GGCCGCACAG CCAATCACGA
    CACGAGCACC TACACAACCA GTACAACAAA GTGAAGGAGG AGGAGGACCA GTGGCAGGAC
    GATTTGGCTC GTTGGAAGAA CCGGAGGCGA AGCGCTTCTC AAGATCTGAT CAAAAAGGAG
    GAGGAGAGGA AGATGATGGA GAAGCTAATG ACAACGGATG GAGGACACGG ACATCGCAAG
    AAGAGCATCA AAACATACAA AGAAATTGTA GAGGAGAAAG AGAAAAGGGA ACAGGAGCTG
    CATGAACAAT ACTGTAAGGC CTCCAGTCCA GAAGAGAAAG CAGCTGTGCT TCAGCGATAT
    GCTCTCCGTT TCACCATCAG CGATGCCATA CTGGAGAAAC TCCAGCTCCC CAAACTGCCT
    TCTGCCGCCT CACCGATGCA CACGTCCCCC CTCCATCAGC CCGAGCAAAA GACCACATCC
    ACTACATTAG TAGAAGCAGA GGTTCCAGAA CTTTCACAAA CTACCCAAAC ACAACAGACT
    GTCCATGTAA AGCAGAAGAT TACACATGAT GAGGCACCGG CACAGCCTAA AATCCCCAAA
    CCTACACCAC CTCCCAAGTG CATCAAAGCT CCAGAAGTCC TGTCGGCACC TTCACGAGTT
    CCTCCAGCTC CATCAAAGCC TGTACCCCTG ATCACCCCCA AACCCTACAG CCAGCCCAAA
    TTCAGCCAAG GCTTCCGCAA ATCGTTGAAG AGTGATGGGC TGATACGTGT GAACGGAGAC
    CACAATCAGC AGGTGATAAG AGAGAAGGCT AATAGAGATC CTCTGCAGAG CCCTGAAGTC
    AACTTCTCTT CCTCACCTCC AAAGCCAGAA ATTCAAAGTC AACAGAATGA GGATCCAATC
    GTGTTAGAAA CCACAGACCA CCAGCAAGAC CTACAGACAC CTAAACCTGA CCTGGAGAAA
    GAAGTCATAA AGAAGGAGGA AGAAAGTCTG CTTAGTCCTC AGAAAGAGAT CAGCAAAGAA
    GACACCTCAA CTACACTTTC AGTGTCAGAA CCAGCAGGAA AGCAGCCAGT GCCTGACACA
    GTGATGAAGC GCAACTTTGT TGTCACAACA ACCATAGTGA CAGAACTCAC TCAGACGCAC
    CCCGTCCCAC TAGAGGACAC GTCCAGCACA GAGCAGCCTG ATACAGTCCA AAATACATGT
    GATCTGAGCT CAACTGCAGA GTCCTGTAAA GCTGATCAGT CTTCATACTC GATCTCGTCG
    ACAGAGGGGA TCGACTACTC CATTGAATGT ATTGAAACCC CAATGTTGAA CCTTGCAAAA
    AGGGTTGACC ACTGGACGTG GGATCCCAAT GAGGAGCGCA GGCGGCAGGA GAGGTGGCAG
    CAGGAGCAGG AGCGTCTCCT GCAGGAGAAA TATCAGCGCG AGCAGGAGAA ACTGAAGGAG
    GAATGGGAGA AAGCCCAGAG AGAAGTAGAA GAGGAAGAAA GGAGACACCA TGAAGAGGAG
    AGACGAATTC TGGAGGAGAC AGTGACCCCG CTGACCCCTC GCACCTCAGC GTTATCATCC
    AGCTGCGGGA CAGAGGCTCC GTCCCTCACA CAGCCCTCAG CTCCACATGA AACCATTGTC
    CTCTCATTGG CCGACTGGGA AAGGAAACAG GAAATGCTGG AGAAACAGGC ACAGACGGGC
    CAGAGTAACG GATTGACACA CAGTCCTGAT CCAGTAATTG CAGCAGCTCA GCAGAACGGA
    CAAAGAGCTG AAGCTCAGGA GAGCCAGATC ACAGCTACAC CACAACTGCA GTTCATACAG
    GATGGCTCGT GGAGCTGCAA GTCTAAAGGC AGGCAGGAGG ATCTGAAGAA AACCGCCTCG
    CTGGATCGTA AACAGAGTCC AGCCCCAAGC CAGACCACAG GGATGAGGAG GACTGGGTCA
    TGTGAGAATG TTTTAGGAAC ACACCCATCA AAATCACCCA CACCATCACA AGACACGCCA
    CCTCCATCAC CCAGCAGGTC AGTCAGTGGA AAGAAGCTTT GCTCCAGTTG CGCTCATCCA
    CTGGGTAAAG GAGCAGCAAT GATCATTGAA ACCTTGGGGC TTTACTTCCA CATCCAGTGT
    TTTAAGTGTG GCGTATGTAA AGGATTACTT GGAGACACAA GTGCTGGAAC TGACGTCAGG
    ATTCGAAATG GACTTCTTAA TTGTCACGAG TGCTACATAA AATCAAGGGC TGCTGGTCAG
    CCAACAACGT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147261
    Clone ID Related Accession (Same CDS sequence) XM_021475278.1
    Accession Version XM_021475278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTGGAGACGT AAACCCTGAG CCTGCTCCTA TCATGCCTCC TGAACCTCCA
    AACCCGTTCA TGTTTGACCT TAAACGGCCT TTGACCTCCA CCGAGCGCAG GGTGCTGGAG
    GATGAACTGG TTGAGAAAGC AAAGGATGAG AAGGATGATG ATGAAGAAGA CGAAGCCGAA
    GAAAGGCAGC CGGATATTGA AAAGGATGAT ATGCTAGCTC GCAGAACAAG AGCATTTAAG
    AAGACAGCGG GTGGGACTTC GTTTAACCAG TTCCTCCCGC TGCCGGGGTC CAAGAGAGAG
    GGAACTGTAG GTGGTTCTTT AGCCCAGAAA GGAGGTGTAC TGATAGGCTC GAGTGGAGAC
    AGAGACCTGC ACTACCTGGA GAGAAATATA AGGACTAAGA GAACCAAGAT TGGACAGAGA
    TTTGAACCAG TCGATCAAGT AGCAAAGACA CCGGTTGCTG TAGTCACCCA GCAGAGCCCT
    GATATTGTGC GCTCTACTGC ATCTAACACT GATCGCTCTG CAAGTTGCGA TTATGATGAT
    GATGATGATG ATGAATATGT GGATGACGGT CAATCTCCAT ATGTTGAGAA GGATGATATG
    CTTGCGCGAA GAACTGGATC CTATCAAAAG CCAAACGCAG GGGCAGGACA GTCTTTTAAT
    GCATTTCTGC CCAAACCTGG TGCTGTCAAA CATAAAATCA CCCCTGTCAG CGGATCACAG
    TCTTGCGTTA AGCCAAGAGA GCATGAGACA GCCCCATCAC AGGACAGTAA CCATGGCCAC
    TCTCTCAGCT TCACCCTTAG CTCTGTGGCC CCGCCCCCAA GAGATGCTGA GGACATGCCC
    AGGAAGTGGC CTCAGATGAT GGACAGGCAG GAGGCGGGCT CTGGGGGACA GGAAAGTCTT
    ATTAAACATG AGACTATTAC CAAAAGCCTG TCCCCTATGC CTGAGCAACC GTCCCGTCGG
    CCCCTTTGGC AGGAAGATGA TGATGATCTA CCCCCAATAT TCAGAGCTAC TAGTGTTTCT
    GATGAAGCGG AGAGCGTGAG TTTGATTGAC ATGCGTGATG AAGAGGATGA GCTTGCATAC
    CTTCGGCCGC ACAGCCAATC ACGACACGAG CACCTACACA ACCAGTACAA CAAAGTGAAG
    GAGGAGGAGG ACCAGTGGCA GGACGATTTG GCTCGTTGGA AGAACCGGAG GCGAAGCGCT
    TCTCAAGATC TGATCAAAAA GGAGGAGGAG AGGAAGATGA TGGAGAAGCT AATGACAACG
    GATGGAGGAC ACGGACATCG CAAGAAGAGC ATCAAAACAT ACAAAGAAAT TGTAGAGGAG
    AAAGAGAAAA GGGAACAGGA GCTGCATGAA CAATACTGTA AGGCCTCCAG TCCAGAAGAG
    AAAGCAGCTG TGCTTCAGCG ATATGCTCTC CGTTTCACCA TCAGCGATGC CATACTGGAG
    AAACTCCAGC TCCCCAAACT GCCTTCTGCC GCCTCACCGA TGCACACGTC CCCCCTCCAT
    CAGCCCGAGC AAAAGACCAC ATCCACTACA TTAGTAGAAG CAGAGGTTCC AGAACTTTCA
    CAAACTACCC AAACACAACA GACTGTCCAT GTAAAGCAGA AGATTACACA TGATGAGGCA
    CCGGCACAGC CTAAAATCCC CAAACCTACA CCACCTCCCA AGTGCATCAA AGCTCCAGAA
    GTCCTGTCGG CACCTTCACG AGTTCCTCCA GCTCCATCAA AGCCTGTACC CCTGATCACC
    CCCAAACCCT ACAGCCAGCC CAAATTCAGC CAAGGCTTCC GCAAATCGTT GAAGAGTGAT
    GGGCTGATAC GTGTGAACGG AGACCACAAT CAGCAGGTGA TAAGAGAGAA GGCTAATAGA
    GATCCTCTGC AGAGCCCTGA AGTCAACTTC TCTTCCTCAC CTCCAAAGCC AGAAATTCAA
    AGTCAACAGA ATGAGGATCC AATCGTGTTA GAAACCACAG ACCACCAGCA AGACCTACAG
    ACACCTAAAC CTGACCTGGA GAAAGAAGTC ATAAAGAAGG AGGAAGAAAG TCTGCTTAGT
    CCTCAGAAAG AGATCAGCAA AGAAGACACC TCAACTACAC TTTCAGTGTC AGAACCAGCA
    GGAAAGCAGC CAGTGCCTGA CACAGTGATG AAGCGCAACT TTGTTGTCAC AACAACCATA
    GTGACAGAAC TCACTCAGAC GCACCCCGTC CCACTAGAGG ACACGTCCAG CACAGAGCAG
    CCTGATACAG TCCAAAATAC ATGTGATCTG AGCTCAACTG CAGAGTCCTG TAAAGCTGAT
    CAGTCTTCAT ACTCGATCTC GTCGACAGAG GGGATCGACT ACTCCATTGA ATGTATTGAA
    ACCCCAATGT TGAACCTTGC AAAAAGGGTT GACCACTGGA CGTGGGATCC CAATGAGGAG
    CGCAGGCGGC AGGAGAGGTG GCAGCAGGAG CAGGAGCGTC TCCTGCAGGA GAAATATCAG
    CGCGAGCAGG AGAAACTGAA GGAGGAATGG GAGAAAGCCC AGAGAGAAGT AGAAGAGGAA
    GAAAGGAGAC ACCATGAAGA GGAGAGACGA ATTCTGGAGG AGACAGTGAC CCCGCTGACC
    CCTCGCACCT CAGCGTTATC ATCCAGCTGC GGGACAGAGG CTCCGTCCCT CACACAGCCC
    TCAGCTCCAC ATGAAACCAT TGTCCTCTCA TTGGCCGACT GGGAAAGGAA ACAGGAAATG
    CTGGAGAAAC AGGCACAGAC GGGCCAGAGT AACGGATTGA CACACAGTCC TGATCCAGTA
    ATTGCAGCAG CTCAGCAGAA CGGACAAAGA GCTGAAGCTC AGGAGAGCCA GATCACAGCT
    ACACCACAAC TGCAGTTCAT ACAGGATGGC TCGTGGAGCT GCAAGTCTAA AGGCAGGCAG
    GAGGATCTGA AGAAAACCGC CTCGCTGGAT CGTAAACAGA GTCCAGCCCC AAGCCAGACC
    ACAGGGATGA GGAGGACTGG GTCATGTGAG AATGTTTTAG GAACACACCC ATCAAAATCA
    CCCACACCAT CACAAGACAC GCCACCTCCA TCACCCAGCA GGTCAGTCAG TGGAAAGAAG
    CTTTGCTCCA GTTGCGCTCA TCCACTGGGT AAAGGAGCAG CAATGATCAT TGAAACCTTG
    GGGCTTTACT TCCACATCCA GTGTTTTAAG TGTGGCGTAT GTAAAGGATT ACTTGGAGAC
    ACAAGTGCTG GAACTGACGT CAGGATTCGA AATGGACTTC TTAATTGTCA CGAGTGCTAC
    ATAAAATCAA GGGCTGCTGG TCAGCCAACA ACGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330953.1
    CDS365..5083
    Translation

    Target ORF information:

    RefSeq Version XM_021475278.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTGGAGACGT AAACCCTGAG CCTGCTCCTA TCATGCCTCC TGAACCTCCA
    AACCCGTTCA TGTTTGACCT TAAACGGCCT TTGACCTCCA CCGAGCGCAG GGTGCTGGAG
    GATGAACTGG TTGAGAAAGC AAAGGATGAG AAGGATGATG ATGAAGAAGA CGAAGCCGAA
    GAAAGGCAGC CGGATATTGA AAAGGATGAT ATGCTAGCTC GCAGAACAAG AGCATTTAAG
    AAGACAGCGG GTGGGACTTC GTTTAACCAG TTCCTCCCGC TGCCGGGGTC CAAGAGAGAG
    GGAACTGTAG GTGGTTCTTT AGCCCAGAAA GGAGGTGTAC TGATAGGCTC GAGTGGAGAC
    AGAGACCTGC ACTACCTGGA GAGAAATATA AGGACTAAGA GAACCAAGAT TGGACAGAGA
    TTTGAACCAG TCGATCAAGT AGCAAAGACA CCGGTTGCTG TAGTCACCCA GCAGAGCCCT
    GATATTGTGC GCTCTACTGC ATCTAACACT GATCGCTCTG CAAGTTGCGA TTATGATGAT
    GATGATGATG ATGAATATGT GGATGACGGT CAATCTCCAT ATGTTGAGAA GGATGATATG
    CTTGCGCGAA GAACTGGATC CTATCAAAAG CCAAACGCAG GGGCAGGACA GTCTTTTAAT
    GCATTTCTGC CCAAACCTGG TGCTGTCAAA CATAAAATCA CCCCTGTCAG CGGATCACAG
    TCTTGCGTTA AGCCAAGAGA GCATGAGACA GCCCCATCAC AGGACAGTAA CCATGGCCAC
    TCTCTCAGCT TCACCCTTAG CTCTGTGGCC CCGCCCCCAA GAGATGCTGA GGACATGCCC
    AGGAAGTGGC CTCAGATGAT GGACAGGCAG GAGGCGGGCT CTGGGGGACA GGAAAGTCTT
    ATTAAACATG AGACTATTAC CAAAAGCCTG TCCCCTATGC CTGAGCAACC GTCCCGTCGG
    CCCCTTTGGC AGGAAGATGA TGATGATCTA CCCCCAATAT TCAGAGCTAC TAGTGTTTCT
    GATGAAGCGG AGAGCGTGAG TTTGATTGAC ATGCGTGATG AAGAGGATGA GCTTGCATAC
    CTTCGGCCGC ACAGCCAATC ACGACACGAG CACCTACACA ACCAGTACAA CAAAGTGAAG
    GAGGAGGAGG ACCAGTGGCA GGACGATTTG GCTCGTTGGA AGAACCGGAG GCGAAGCGCT
    TCTCAAGATC TGATCAAAAA GGAGGAGGAG AGGAAGATGA TGGAGAAGCT AATGACAACG
    GATGGAGGAC ACGGACATCG CAAGAAGAGC ATCAAAACAT ACAAAGAAAT TGTAGAGGAG
    AAAGAGAAAA GGGAACAGGA GCTGCATGAA CAATACTGTA AGGCCTCCAG TCCAGAAGAG
    AAAGCAGCTG TGCTTCAGCG ATATGCTCTC CGTTTCACCA TCAGCGATGC CATACTGGAG
    AAACTCCAGC TCCCCAAACT GCCTTCTGCC GCCTCACCGA TGCACACGTC CCCCCTCCAT
    CAGCCCGAGC AAAAGACCAC ATCCACTACA TTAGTAGAAG CAGAGGTTCC AGAACTTTCA
    CAAACTACCC AAACACAACA GACTGTCCAT GTAAAGCAGA AGATTACACA TGATGAGGCA
    CCGGCACAGC CTAAAATCCC CAAACCTACA CCACCTCCCA AGTGCATCAA AGCTCCAGAA
    GTCCTGTCGG CACCTTCACG AGTTCCTCCA GCTCCATCAA AGCCTGTACC CCTGATCACC
    CCCAAACCCT ACAGCCAGCC CAAATTCAGC CAAGGCTTCC GCAAATCGTT GAAGAGTGAT
    GGGCTGATAC GTGTGAACGG AGACCACAAT CAGCAGGTGA TAAGAGAGAA GGCTAATAGA
    GATCCTCTGC AGAGCCCTGA AGTCAACTTC TCTTCCTCAC CTCCAAAGCC AGAAATTCAA
    AGTCAACAGA ATGAGGATCC AATCGTGTTA GAAACCACAG ACCACCAGCA AGACCTACAG
    ACACCTAAAC CTGACCTGGA GAAAGAAGTC ATAAAGAAGG AGGAAGAAAG TCTGCTTAGT
    CCTCAGAAAG AGATCAGCAA AGAAGACACC TCAACTACAC TTTCAGTGTC AGAACCAGCA
    GGAAAGCAGC CAGTGCCTGA CACAGTGATG AAGCGCAACT TTGTTGTCAC AACAACCATA
    GTGACAGAAC TCACTCAGAC GCACCCCGTC CCACTAGAGG ACACGTCCAG CACAGAGCAG
    CCTGATACAG TCCAAAATAC ATGTGATCTG AGCTCAACTG CAGAGTCCTG TAAAGCTGAT
    CAGTCTTCAT ACTCGATCTC GTCGACAGAG GGGATCGACT ACTCCATTGA ATGTATTGAA
    ACCCCAATGT TGAACCTTGC AAAAAGGGTT GACCACTGGA CGTGGGATCC CAATGAGGAG
    CGCAGGCGGC AGGAGAGGTG GCAGCAGGAG CAGGAGCGTC TCCTGCAGGA GAAATATCAG
    CGCGAGCAGG AGAAACTGAA GGAGGAATGG GAGAAAGCCC AGAGAGAAGT AGAAGAGGAA
    GAAAGGAGAC ACCATGAAGA GGAGAGACGA ATTCTGGAGG AGACAGTGAC CCCGCTGACC
    CCTCGCACCT CAGCGTTATC ATCCAGCTGC GGGACAGAGG CTCCGTCCCT CACACAGCCC
    TCAGCTCCAC ATGAAACCAT TGTCCTCTCA TTGGCCGACT GGGAAAGGAA ACAGGAAATG
    CTGGAGAAAC AGGCACAGAC GGGCCAGAGT AACGGATTGA CACACAGTCC TGATCCAGTA
    ATTGCAGCAG CTCAGCAGAA CGGACAAAGA GCTGAAGCTC AGGAGAGCCA GATCACAGCT
    ACACCACAAC TGCAGTTCAT ACAGGATGGC TCGTGGAGCT GCAAGTCTAA AGGCAGGCAG
    GAGGATCTGA AGAAAACCGC CTCGCTGGAT CGTAAACAGA GTCCAGCCCC AAGCCAGACC
    ACAGGGATGA GGAGGACTGG GTCATGTGAG AATGTTTTAG GAACACACCC ATCAAAATCA
    CCCACACCAT CACAAGACAC GCCACCTCCA TCACCCAGCA GGTCAGTCAG TGGAAAGAAG
    CTTTGCTCCA GTTGCGCTCA TCCACTGGGT AAAGGAGCAG CAATGATCAT TGAAACCTTG
    GGGCTTTACT TCCACATCCA GTGTTTTAAG TGTGGCGTAT GTAAAGGATT ACTTGGAGAC
    ACAAGTGCTG GAACTGACGT CAGGATTCGA AATGGACTTC TTAATTGTCA CGAGTGCTAC
    ATAAAATCAA GGGCTGCTGG TCAGCCAACA ACGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53710
    Clone ID Related Accession (Same CDS sequence) XM_017355096.1
    Accession Version XM_017355096.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGCTTCACC
    CTTAGCTCTG TGGCCCCGCC CCCAAGAGAT GCTGAGGACA TGCCCAGGAA GTGGCCTCAG
    ATGATGGACA GGCAGGAGGC GGGCTCTGGG GGACAGGAAA GTCTTATTAA ACATGAGACT
    ATTACCAAAA GCCTGTCCCC TATGCCTGAG CAACCGTCCC GTCGGCCCCT TTGGCAGGAA
    GATGATGATG ATCTACCCCC AATATTCAGA GCTACTAGTG TTTCTGATGA AGCGGAGAGC
    GTGAGTTTGA TTGACATGCG TGATGAAGAG GATGAGCTTG CATACCTTCG GCCGCACAGC
    CAATCACGAC ACGAGCACCT ACACAACCAG TACAACAAAG TGAAGGAGGA GGAGGACCAG
    TGGCAGGACG ATTTGGCTCG TTGGAAGAAC CGGAGGCGAA GCGCTTCTCA AGATCTGATC
    AAAAAGGAGG AGGAGAGGAA GATGATGGAG AAGCTAATGA CAACGGATGG AGGACACGGA
    CATCGCAAGA AGAGCATCAA AACATACAAA GAAATTGTAG AGGAGAAAGA GAAAAGGGAA
    CAGGAGCTGC ATGAACAATA CTGTAAGGCC TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT
    CAGCGATATG CTCTCCGTTT CACCATCAGC GATGCCATAC TGGAGAAACT CCAGCTCCCC
    AAACTGCCTT CTGCCGCCTC ACCGATGCAC ACGTCCCCCC TCCATCAGCC CGAGCAAAAG
    ACCACATCCA CTACATTAGT AGAAGCAGAG GTTCCAGAAC TTTCACAAAC TACCCAAACA
    CAACAGACTG TCCATGTAAA GCAGAAGATT ACACATGATG AGGCACCGGC ACAGCCTAAA
    ATCCCCAAAC CTACACCACC TCCCAAGTGC ATCAAAGCTC CAGAAGTCCT GTCGGCACCT
    TCACGAGTTC CTCCAGCTCC ATCAAAGCCT GTACCCCTGA TCACCCCCAA ACCCTACAGC
    CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA TCGTTGAAGA GTGATGGGCT GATACGTGTG
    AACGGAGACC ACAATCAGCA GGTGATAAGA GAGAAGGCTA ATAGAGATCC TCTGCAGAGC
    CCTGAAGTCA ACTTCTCTTC CTCACCTCCA AAGCCAGAAA TTCAAAGTCA ACAGAATGAG
    GATCCAATCG TGTTAGAAAC CACAGACCAC CAGCAAGACC TACAGACACC TAAACCTGAC
    CTGGAGAAAG AAGTCATAAA GAAGGAGGAA GAAAGTCTGC TTAGTCCTCA GAAAGAGATC
    AGCAAAGAAG ACACCTCAAC TACACTTTCA GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG
    CCTGACACAG TGATGAAGCG CAACTTTGTT GTCACAACAA CCATAGTGAC AGAACTCACT
    CAGACGCACC CCGTCCCACT AGAGGACACG TCCAGCACAG AGCAGCCTGA TACAGTCCAA
    AATACATGTG ATCTGAGCTC AACTGCAGAG TCCTGTAAAG CTGATCAGTC TTCATACTCG
    ATCTCGTCGA CAGAGGGGAT CGACTACTCC ATTGAATGTA TTGAAACCCC AATGTTGAAC
    CTTGCAAAAA GGGTTGACCA CTGGACGTGG GATCCCAATG AGGAGCGCAG GCGGCAGGAG
    AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA
    CTGAAGGAGG AATGGGAGAA AGCCCAGAGA GAAGTAGAAG AGGAAGAAAG GAGACACCAT
    GAAGAGGAGA GACGAATTCT GGAGGAGACA GTGACCCCGC TGACCCCTCG CACCTCAGCG
    TTATCATCCA GCTGCGGGAC AGAGGCTCCG TCCCTCACAC AGCCCTCAGC TCCACATGAA
    ACCATTGTCC TCTCATTGGC CGACTGGGAA AGGAAACAGG AAATGCTGGA GAAACAGGCA
    CAGACGGGCC AGAGTAACGG ATTGACACAC AGTCCTGATC CAGTAATTGC AGCAGCTCAG
    CAGAACGGAC AAAGAGCTGA AGCTCAGGAG AGCCAGATCA CAGCTACACC ACAACTGCAG
    TTCATACAGG ATGGCTCGTG GAGCTGCAAG TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA
    ACCGCCTCGC TGGATCGTAA ACAGAGTCCA GCCCCAAGCC AGACCACAGG GATGAGGAGG
    ACTGGGTCAT GTGAGAATGT TTTAGGAACA CACCCATCAA AATCACCCAC ACCATCACAA
    GACACGCCAC CTCCATCACC CAGCAGGTCA GTCAGTGGAA AGAAGCTTTG CTCCAGTTGC
    GCTCATCCAC TGGGTAAAGG AGCAGCAATG ATCATTGAAA CCTTGGGGCT TTACTTCCAC
    ATCCAGTGTT TTAAGTGTGG CGTATGTAAA GGATTACTTG GAGACACAAG TGCTGGAACT
    GACGTCAGGA TTCGAAATGG ACTTCTTAAT TGTCACGAGT GCTACATAAA ATCAAGGGCT
    GCTGGTCAGC CAACAACGTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210585.1
    CDS375..5918
    Translation

    Target ORF information:

    RefSeq Version XM_017355096.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355096.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGCTTCACC
    CTTAGCTCTG TGGCCCCGCC CCCAAGAGAT GCTGAGGACA TGCCCAGGAA GTGGCCTCAG
    ATGATGGACA GGCAGGAGGC GGGCTCTGGG GGACAGGAAA GTCTTATTAA ACATGAGACT
    ATTACCAAAA GCCTGTCCCC TATGCCTGAG CAACCGTCCC GTCGGCCCCT TTGGCAGGAA
    GATGATGATG ATCTACCCCC AATATTCAGA GCTACTAGTG TTTCTGATGA AGCGGAGAGC
    GTGAGTTTGA TTGACATGCG TGATGAAGAG GATGAGCTTG CATACCTTCG GCCGCACAGC
    CAATCACGAC ACGAGCACCT ACACAACCAG TACAACAAAG TGAAGGAGGA GGAGGACCAG
    TGGCAGGACG ATTTGGCTCG TTGGAAGAAC CGGAGGCGAA GCGCTTCTCA AGATCTGATC
    AAAAAGGAGG AGGAGAGGAA GATGATGGAG AAGCTAATGA CAACGGATGG AGGACACGGA
    CATCGCAAGA AGAGCATCAA AACATACAAA GAAATTGTAG AGGAGAAAGA GAAAAGGGAA
    CAGGAGCTGC ATGAACAATA CTGTAAGGCC TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT
    CAGCGATATG CTCTCCGTTT CACCATCAGC GATGCCATAC TGGAGAAACT CCAGCTCCCC
    AAACTGCCTT CTGCCGCCTC ACCGATGCAC ACGTCCCCCC TCCATCAGCC CGAGCAAAAG
    ACCACATCCA CTACATTAGT AGAAGCAGAG GTTCCAGAAC TTTCACAAAC TACCCAAACA
    CAACAGACTG TCCATGTAAA GCAGAAGATT ACACATGATG AGGCACCGGC ACAGCCTAAA
    ATCCCCAAAC CTACACCACC TCCCAAGTGC ATCAAAGCTC CAGAAGTCCT GTCGGCACCT
    TCACGAGTTC CTCCAGCTCC ATCAAAGCCT GTACCCCTGA TCACCCCCAA ACCCTACAGC
    CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA TCGTTGAAGA GTGATGGGCT GATACGTGTG
    AACGGAGACC ACAATCAGCA GGTGATAAGA GAGAAGGCTA ATAGAGATCC TCTGCAGAGC
    CCTGAAGTCA ACTTCTCTTC CTCACCTCCA AAGCCAGAAA TTCAAAGTCA ACAGAATGAG
    GATCCAATCG TGTTAGAAAC CACAGACCAC CAGCAAGACC TACAGACACC TAAACCTGAC
    CTGGAGAAAG AAGTCATAAA GAAGGAGGAA GAAAGTCTGC TTAGTCCTCA GAAAGAGATC
    AGCAAAGAAG ACACCTCAAC TACACTTTCA GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG
    CCTGACACAG TGATGAAGCG CAACTTTGTT GTCACAACAA CCATAGTGAC AGAACTCACT
    CAGACGCACC CCGTCCCACT AGAGGACACG TCCAGCACAG AGCAGCCTGA TACAGTCCAA
    AATACATGTG ATCTGAGCTC AACTGCAGAG TCCTGTAAAG CTGATCAGTC TTCATACTCG
    ATCTCGTCGA CAGAGGGGAT CGACTACTCC ATTGAATGTA TTGAAACCCC AATGTTGAAC
    CTTGCAAAAA GGGTTGACCA CTGGACGTGG GATCCCAATG AGGAGCGCAG GCGGCAGGAG
    AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA
    CTGAAGGAGG AATGGGAGAA AGCCCAGAGA GAAGTAGAAG AGGAAGAAAG GAGACACCAT
    GAAGAGGAGA GACGAATTCT GGAGGAGACA GTGACCCCGC TGACCCCTCG CACCTCAGCG
    TTATCATCCA GCTGCGGGAC AGAGGCTCCG TCCCTCACAC AGCCCTCAGC TCCACATGAA
    ACCATTGTCC TCTCATTGGC CGACTGGGAA AGGAAACAGG AAATGCTGGA GAAACAGGCA
    CAGACGGGCC AGAGTAACGG ATTGACACAC AGTCCTGATC CAGTAATTGC AGCAGCTCAG
    CAGAACGGAC AAAGAGCTGA AGCTCAGGAG AGCCAGATCA CAGCTACACC ACAACTGCAG
    TTCATACAGG ATGGCTCGTG GAGCTGCAAG TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA
    ACCGCCTCGC TGGATCGTAA ACAGAGTCCA GCCCCAAGCC AGACCACAGG GATGAGGAGG
    ACTGGGTCAT GTGAGAATGT TTTAGGAACA CACCCATCAA AATCACCCAC ACCATCACAA
    GACACGCCAC CTCCATCACC CAGCAGGTCA GTCAGTGGAA AGAAGCTTTG CTCCAGTTGC
    GCTCATCCAC TGGGTAAAGG AGCAGCAATG ATCATTGAAA CCTTGGGGCT TTACTTCCAC
    ATCCAGTGTT TTAAGTGTGG CGTATGTAAA GGATTACTTG GAGACACAAG TGCTGGAACT
    GACGTCAGGA TTCGAAATGG ACTTCTTAAT TGTCACGAGT GCTACATAAA ATCAAGGGCT
    GCTGGTCAGC CAACAACGTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53711
    Clone ID Related Accession (Same CDS sequence) XM_017355097.1
    Accession Version XM_017355097.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGCTTCACC
    CTTAGCTCTG TGGCCCCGCC CCCAAGAGAT GCTGAGGACA TGCCCAGGAA GTGGCCTCAG
    ATGATGGACA GGCAGGAGGC GGGCTCTGGG GGACAGGAAA GTCTTATTAA ACATGAGACT
    ATTACCAAAA GCCTGTCCCC TATGCCTGAG CAACCGTCCC GTCGGCCCCT TTGGCAGGAA
    GATGATGATG ATCTACCCCC AATATTCAGA GCTACTAGTG TTTCTGATGA AGCGGAGAGC
    GTGAGTTTGA TTGACATGCG TGATGAAGAG GATGAGCTTG CATACCTTCG GCCGCACAGC
    CAATCACGAC ACGAGCACCT ACACAACCAG TACAACAAAG TGAAGGAGGA GGAGGACCAG
    TGGCAGGACG ATTTGGCTCG TTGGAAGAAC CGGAGGCGAA GCGCTTCTCA AGATCTGATC
    AAAAAGGAGG AGGAGAGGAA GATGATGGAG AAGCTAATGA CAACGGATGG AGGACACGGA
    CATCGCAAGA AGAGCATCAA AACATACAAA GAAATTGTAG AGGAGAAAGA GAAAAGGGAA
    CAGGAGCTGC ATGAACAATA CTGTAAGGCC TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT
    CAGCGATATG CTCTCCGTTT CACCATCAGC GATGCCATAC TGGAGAAACT CCAGCTCCCC
    AAACTGCCTT CTGCCGCCTC ACCGATGCAC ACGTCCCCCC TCCATCAGCC CGAGCAAAAG
    ACCACATCCA CTACATTAGT AGAAGCAGAG GTTCCAGAAC TTTCACAAAC TACCCAAACA
    CAACAGACTG TCCATGTAAA GCAGAAGATT ACACATGATG AGGCACCGGC ACAGCCTAAA
    ATCCCCAAAC CTACACCACC TCCCAAGTGC ATCAAAGCTC CAGAAGTCCT GTCGGCACCT
    TCACGAGTTC CTCCAGCTCC ATCAAAGCCT GTACCCCTGA TCACCCCCAA ACCCTACAGC
    CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA TCGTTGAAGA GTGATGGGCT GATACGTGTG
    AACGGAGACC ACAATCAGCA GGTGATAAGA GAGAAGGCTA ATAGAGATCC TCTGCAGAGC
    CCTGAAGTCA ACTTCTCTTC CTCACCTCCA AAGCCAGAAA TTCAAAGTCA ACAGAATGAG
    GATCCAATCG TGTTAGAAAC CACAGACCAC CAGCAAGACC TACAGACACC TAAACCTGAC
    CTGGAGAAAG AAGTCATAAA GAAGGAGGAA GAAAGTCTGC TTAGTCCTCA GAAAGAGATC
    AGCAAAGAAG ACACCTCAAC TACACTTTCA GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG
    CCTGACACAG TGATGAAGCG CAACTTTGTT GTCACAACAA CCATAGTGAC AGAACTCACT
    CAGACGCACC CCGTCCCACT AGAGGACACG TCCAGCACAG AGCAGCCTGA TACAGTCCAA
    AATACATGTG ATCTGAGCTC AACTGCAGAG TCCTGTAAAG CTGATCAGTC TTCATACTCG
    ATCTCGTCGA CAGAGGGGAT CGACTACTCC ATTGAATGTA TTGAAACCCC AATGTTGAAC
    CTTGCAAAAA GGGTTGACCA CTGGACGTGG GATCCCAATG AGGAGCGCAG GCGGCAGGAG
    AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA
    CTGAAGGAGG AATGGGAGAA AGCCCAGAGA GAAGTAGAAG AGGAAGAAAG GAGACACCAT
    GAAGAGGAGA GACGAATTCT GGAGGAGACA GTGACCCCGC TGACCCCTCG CACCTCAGCG
    TTATCATCCA GCTGCGGGAC AGAGGCTCCG TCCCTCACAC AGCCCTCAGC TCCACATGAA
    ACCATTGTCC TCTCATTGGC CGACTGGGAA AGGAAACAGG AAATGCTGGA GAAACAGGCA
    CAGACGGGCC AGAGTAACGG ATTGACACAC AGTCCTGATC CAGTAATTGC AGCAGCTCAG
    CAGAACGGAC AAAGAGCTGA AGCTCAGGAG AGCCAGATCA CAGCTACACC ACAACTGCAG
    TTCATACAGG ATGGCTCGTG GAGCTGCAAG TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA
    ACCGCCTCGC TGGATCGTAA ACAGAGTCCA GCCCCAAGCC AGACCACAGG GATGAGGAGG
    TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT TGCGCTCATC CACTGGGTAA AGGAGCAGCA
    ATGATCATTG AAACCTTGGG GCTTTACTTC CACATCCAGT GTTTTAAGTG TGGCGTATGT
    AAAGGATTAC TTGGAGACAC AAGTGCTGGA ACTGACGTCA GGATTCGAAA TGGACTTCTT
    AATTGTCACG AGTGCTACAT AAAATCAAGG GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210586.1
    CDS376..5832
    Translation

    Target ORF information:

    RefSeq Version XM_017355097.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355097.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGCTTCACC
    CTTAGCTCTG TGGCCCCGCC CCCAAGAGAT GCTGAGGACA TGCCCAGGAA GTGGCCTCAG
    ATGATGGACA GGCAGGAGGC GGGCTCTGGG GGACAGGAAA GTCTTATTAA ACATGAGACT
    ATTACCAAAA GCCTGTCCCC TATGCCTGAG CAACCGTCCC GTCGGCCCCT TTGGCAGGAA
    GATGATGATG ATCTACCCCC AATATTCAGA GCTACTAGTG TTTCTGATGA AGCGGAGAGC
    GTGAGTTTGA TTGACATGCG TGATGAAGAG GATGAGCTTG CATACCTTCG GCCGCACAGC
    CAATCACGAC ACGAGCACCT ACACAACCAG TACAACAAAG TGAAGGAGGA GGAGGACCAG
    TGGCAGGACG ATTTGGCTCG TTGGAAGAAC CGGAGGCGAA GCGCTTCTCA AGATCTGATC
    AAAAAGGAGG AGGAGAGGAA GATGATGGAG AAGCTAATGA CAACGGATGG AGGACACGGA
    CATCGCAAGA AGAGCATCAA AACATACAAA GAAATTGTAG AGGAGAAAGA GAAAAGGGAA
    CAGGAGCTGC ATGAACAATA CTGTAAGGCC TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT
    CAGCGATATG CTCTCCGTTT CACCATCAGC GATGCCATAC TGGAGAAACT CCAGCTCCCC
    AAACTGCCTT CTGCCGCCTC ACCGATGCAC ACGTCCCCCC TCCATCAGCC CGAGCAAAAG
    ACCACATCCA CTACATTAGT AGAAGCAGAG GTTCCAGAAC TTTCACAAAC TACCCAAACA
    CAACAGACTG TCCATGTAAA GCAGAAGATT ACACATGATG AGGCACCGGC ACAGCCTAAA
    ATCCCCAAAC CTACACCACC TCCCAAGTGC ATCAAAGCTC CAGAAGTCCT GTCGGCACCT
    TCACGAGTTC CTCCAGCTCC ATCAAAGCCT GTACCCCTGA TCACCCCCAA ACCCTACAGC
    CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA TCGTTGAAGA GTGATGGGCT GATACGTGTG
    AACGGAGACC ACAATCAGCA GGTGATAAGA GAGAAGGCTA ATAGAGATCC TCTGCAGAGC
    CCTGAAGTCA ACTTCTCTTC CTCACCTCCA AAGCCAGAAA TTCAAAGTCA ACAGAATGAG
    GATCCAATCG TGTTAGAAAC CACAGACCAC CAGCAAGACC TACAGACACC TAAACCTGAC
    CTGGAGAAAG AAGTCATAAA GAAGGAGGAA GAAAGTCTGC TTAGTCCTCA GAAAGAGATC
    AGCAAAGAAG ACACCTCAAC TACACTTTCA GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG
    CCTGACACAG TGATGAAGCG CAACTTTGTT GTCACAACAA CCATAGTGAC AGAACTCACT
    CAGACGCACC CCGTCCCACT AGAGGACACG TCCAGCACAG AGCAGCCTGA TACAGTCCAA
    AATACATGTG ATCTGAGCTC AACTGCAGAG TCCTGTAAAG CTGATCAGTC TTCATACTCG
    ATCTCGTCGA CAGAGGGGAT CGACTACTCC ATTGAATGTA TTGAAACCCC AATGTTGAAC
    CTTGCAAAAA GGGTTGACCA CTGGACGTGG GATCCCAATG AGGAGCGCAG GCGGCAGGAG
    AGGTGGCAGC AGGAGCAGGA GCGTCTCCTG CAGGAGAAAT ATCAGCGCGA GCAGGAGAAA
    CTGAAGGAGG AATGGGAGAA AGCCCAGAGA GAAGTAGAAG AGGAAGAAAG GAGACACCAT
    GAAGAGGAGA GACGAATTCT GGAGGAGACA GTGACCCCGC TGACCCCTCG CACCTCAGCG
    TTATCATCCA GCTGCGGGAC AGAGGCTCCG TCCCTCACAC AGCCCTCAGC TCCACATGAA
    ACCATTGTCC TCTCATTGGC CGACTGGGAA AGGAAACAGG AAATGCTGGA GAAACAGGCA
    CAGACGGGCC AGAGTAACGG ATTGACACAC AGTCCTGATC CAGTAATTGC AGCAGCTCAG
    CAGAACGGAC AAAGAGCTGA AGCTCAGGAG AGCCAGATCA CAGCTACACC ACAACTGCAG
    TTCATACAGG ATGGCTCGTG GAGCTGCAAG TCTAAAGGCA GGCAGGAGGA TCTGAAGAAA
    ACCGCCTCGC TGGATCGTAA ACAGAGTCCA GCCCCAAGCC AGACCACAGG GATGAGGAGG
    TCAGTCAGTG GAAAGAAGCT TTGCTCCAGT TGCGCTCATC CACTGGGTAA AGGAGCAGCA
    ATGATCATTG AAACCTTGGG GCTTTACTTC CACATCCAGT GTTTTAAGTG TGGCGTATGT
    AAAGGATTAC TTGGAGACAC AAGTGCTGGA ACTGACGTCA GGATTCGAAA TGGACTTCTT
    AATTGTCACG AGTGCTACAT AAAATCAAGG GCTGCTGGTC AGCCAACAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53712
    Clone ID Related Accession (Same CDS sequence) XM_017355098.1
    Accession Version XM_017355098.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGCTTCACC
    CTTAGCTCTG TGGCCCCGCC CCCAAGAGAT GCTGAGGACA TGCCCAGGAA GTGGCCTCAG
    ATGATGGACA GGCAGGAGGC GGGCTCTGGG GGACAGGAAA GTCTTATTAA ACATGAGACT
    ATTACCAAAA GCCTGTCCCC TATGCCTGAG CAACCGTCCC GTCGGCCCCT TTGGCAGGAA
    GATGATGATG ATCTACCCCC AATATTCAGA GCTACTAGTG TTTCTGATGA AGCGGAGAGC
    GTGAGTTTGA TTGACATGCG TGATGAAGAG GATGAGCTTG CATACCTTCG GCCGCACAGC
    CAATCACGAC ACGAGCACCT ACACAACCAG TACAACAAAG TGAAGGAGGA GGAGGACCAG
    TGGCAGGACG ATTTGGCTCG TTGGAAGAAC CGGAGGCGAA GCGCTTCTCA AGATCTGATC
    AAAAAGGAGG AGGAGAGGAA GATGATGGAG AAGCTAATGA CAACGGATGG AGGACACGGA
    CATCGCAAGA AGAGCATCAA AACATACAAA GAAATTGTAG AGGAGAAAGA GAAAAGGGAA
    CAGGAGCTGC ATGAACAATA CTGTAAGGCC TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT
    CAGCGATATG CTCTCCGTTT CACCATCAGC GATGCCATAC TGGAGAAACT CCAGCTCCCC
    AAACTGCCTT CTGCCGCCTC ACCGATGCAC ACGTCCCCCC TCCATCAGCC CGAGCAAAAG
    ACCACATCCA CTACATTAGT AGAAGCAGAG GTTCCAGAAC TTTCACAAAC TACCCAAACA
    CAACAGACTG TCCATGTAAA GCAGAAGATT ACACATGATG AGGCACCGGC ACAGCCTAAA
    ATCCCCAAAC CTACACCACC TCCCAAGTGC ATCAAAGCTC CAGAAGTCCT GTCGGCACCT
    TCACGAGTTC CTCCAGCTCC ATCAAAGCCT GTACCCCTGA TCACCCCCAA ACCCTACAGC
    CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA TCGTTGAAGA GTGATGGGCT GATACGTGTG
    AACGGAGACC ACAATCAGCA GGTGATAAGA GAGAAGGCTA ATAGAGATCC TCTGCAGAGC
    CCTGAAGTCA ACTTCTCTTC CTCACCTCCA AAGCCAGAAA TTCAAAGTCA ACAGAATGAG
    GATCCAATCG TGTTAGAAAC CACAGACCAC CAGCAAGACC TACAGACACC TAAACCTGAC
    CTGGAGAAAG AAGTCATAAA GAAGGAGGAA GAAAGTCTGC TTAGTCCTCA GAAAGAGATC
    AGCAAAGAAG ACACCTCAAC TACACTTTCA GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG
    CCTGACACAG TGATGAAGCG CAACTTTGTT GTCACAACAA CCATAGTGAC AGAACTCACT
    CAGACGCACC CCGTCCCACT AGAGGACACG TCCAGCACAG AGCAGCCTGA TACAGTCCAA
    AATACATGTG ATCTGAGCTC AACTGCAGAG TCCTGTAAAG CTGATCAGTC TTCATACTCG
    ATCTCGTCGA CAGAGGGGAT CGACTACTCC ATTGAATGTG AGAAATATCA GCGCGAGCAG
    GAGAAACTGA AGGAGGAATG GGAGAAAGCC CAGAGAGAAG TAGAAGAGGA AGAAAGGAGA
    CACCATGAAG AGGAGAGACG AATTCTGGAG GAGACAGTGA CCCCGCTGAC CCCTCGCACC
    TCAGCGTTAT CATCCAGCTG CGGGACAGAG GCTCCGTCCC TCACACAGCC CTCAGCTCCA
    CATGAAACCA TTGTCCTCTC ATTGGCCGAC TGGGAAAGGA AACAGGAAAT GCTGGAGAAA
    CAGGCACAGA CGGGCCAGAG TAACGGATTG ACACACAGTC CTGATCCAGT AATTGCAGCA
    GCTCAGCAGA ACGGACAAAG AGCTGAAGCT CAGGAGAGCC AGATCACAGC TACACCACAA
    CTGCAGTTCA TACAGGATGG CTCGTGGAGC TGCAAGTCTA AAGGCAGGCA GGAGGATCTG
    AAGAAAACCG CCTCGCTGGA TCGTAAACAG AGTCCAGCCC CAAGCCAGAC CACAGGGATG
    AGGAGGACTG GGTCATGTGA GAATGTTTTA GGAACACACC CATCAAAATC ACCCACACCA
    TCACAAGACA CGCCACCTCC ATCACCCAGC AGGTCAGTCA GTGGAAAGAA GCTTTGCTCC
    AGTTGCGCTC ATCCACTGGG TAAAGGAGCA GCAATGATCA TTGAAACCTT GGGGCTTTAC
    TTCCACATCC AGTGTTTTAA GTGTGGCGTA TGTAAAGGAT TACTTGGAGA CACAAGTGCT
    GGAACTGACG TCAGGATTCG AAATGGACTT CTTAATTGTC ACGAGTGCTA CATAAAATCA
    AGGGCTGCTG GTCAGCCAAC AACGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210587.1
    CDS376..5805
    Translation

    Target ORF information:

    RefSeq Version XM_017355098.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTCAGACTGA GTCCGCCTCA CCTGAGGTGC CAATCATAAC TCGAAAGGAC
    AATCCTTTCC TGAGTCTAGA AAAAGACAGG GCAAAAGAGG GAGATGAGGA AGATGAAAGA
    GAGGAAGAGG GGAAGACAGT CATCCCAAAC CCACACAAAG ATGATCTGGC ACGGAGACGG
    ACTGGGGGAA GGCCACGCAT TCCCAAAGAT CCTTACCAGT CACTGGTCCA GACTTCTATC
    ACTCAGTCAG ACCTGGAGAC ATGGCAGAGA CTGAAGATGA ATACAGAGAG CAGTGAGTCT
    GACTCCGCCC CTGCTGAAGT GTCAATCATA ACTCGCAAGG AAAACCCCTT CCTAACCCCT
    GAGAAACAAA GTGAGCAGGA GACTGTAGAG GGGGCAAGCA GAGAGGAAGG GGAGAGGGTG
    ACGACACCCA ATGTAAAGGC AGATGACCTG GCCAGGAGGC GGAGCCAGAG CGGGTCCCTC
    CCACTGAAGG AGCCTCGACA GTCATTGGCT CAAACAACCA TCACTCAGTC AGATCTGGAG
    AAATGGCAGA AGCTCAAGAT GAGTACAGAC AGCAGTGAGT CTGACTCCGC CCCTGCTGAA
    GTGTCAATTA TAACTCGCAA GGAAAACCCC TTCCTGACCC CTGAGAAACA AAGTGAGCAG
    GAGAACGAAG AGAGTGCAAA CAGAGAGGAA GGGGAAAGGG TGATGACGCC CAATGTAAAG
    ACAGATGACC TGGCCAGGAG GCGGAGCCAG AGCGGGTCCC TCCTACAGAA GGAGCCTCGG
    CAGTCATTGG CTCAAACAAC CATCACTCAG ACAGATCTGG AGAAATGGCA GAAGCTCAAG
    ATGAGTACAG ACAGCAGTGG AGACGTAAAC CCTGAGCCTG CTCCTATCAT GCCTCCTGAA
    CCTCCAAACC CGTTCATGTT TGACCTTAAA CGGCCTTTGA CCTCCACCGA GCGCAGGGTG
    CTGGAGGATG AACTGGTTGA GAAAGCAAAG GATGAGAAGG ATGATGATGA AGAAGACGAA
    GCCGAAGAAA GGCAGCCGGA TATTGAAAAG GATGATATGC TAGCTCGCAG AACAAGAGCA
    TTTAAGAAGA CAGCGGGTGG GACTTCGTTT AACCAGTTCC TCCCGCTGCC GGGGTCCAAG
    AGAGAGGGAA CTGTAGGTGG TTCTTTAGCC CAGAAAGGAG GTGTACTGAT AGGCTCGAGT
    GGAGACAGAG ACCTGCACTA CCTGGAGAGA AATATAAGGA CTAAGAGAAC CAAGATTGGA
    CAGAGATTTG AACCAGTCGA TCAAGTAGCA AAGACACCGG TTGCTGTAGT CACCCAGCAG
    AGCCCTGATA TTGTGCGCTC TACTGCATCT AACACTGATC GCTCTGCAAG TTGCGATTAT
    GATGATGATG ATGATGATGA ATATGTGGAT GACGGTCAAT CTCCATATGT TGAGAAGGAT
    GATATGCTTG CGCGAAGAAC TGGATCCTAT CAAAAGCCAA ACGCAGGGGC AGGACAGTCT
    TTTAATGCAT TTCTGCCCAA ACCTGGTGCT GTCAAACATA AAATCACCCC TGTCAGCGGA
    TCACAGTCTT GCGTTAAGCC AAGAGAGCAT GAGACAGCCC CATCACAGGA CAGCTTCACC
    CTTAGCTCTG TGGCCCCGCC CCCAAGAGAT GCTGAGGACA TGCCCAGGAA GTGGCCTCAG
    ATGATGGACA GGCAGGAGGC GGGCTCTGGG GGACAGGAAA GTCTTATTAA ACATGAGACT
    ATTACCAAAA GCCTGTCCCC TATGCCTGAG CAACCGTCCC GTCGGCCCCT TTGGCAGGAA
    GATGATGATG ATCTACCCCC AATATTCAGA GCTACTAGTG TTTCTGATGA AGCGGAGAGC
    GTGAGTTTGA TTGACATGCG TGATGAAGAG GATGAGCTTG CATACCTTCG GCCGCACAGC
    CAATCACGAC ACGAGCACCT ACACAACCAG TACAACAAAG TGAAGGAGGA GGAGGACCAG
    TGGCAGGACG ATTTGGCTCG TTGGAAGAAC CGGAGGCGAA GCGCTTCTCA AGATCTGATC
    AAAAAGGAGG AGGAGAGGAA GATGATGGAG AAGCTAATGA CAACGGATGG AGGACACGGA
    CATCGCAAGA AGAGCATCAA AACATACAAA GAAATTGTAG AGGAGAAAGA GAAAAGGGAA
    CAGGAGCTGC ATGAACAATA CTGTAAGGCC TCCAGTCCAG AAGAGAAAGC AGCTGTGCTT
    CAGCGATATG CTCTCCGTTT CACCATCAGC GATGCCATAC TGGAGAAACT CCAGCTCCCC
    AAACTGCCTT CTGCCGCCTC ACCGATGCAC ACGTCCCCCC TCCATCAGCC CGAGCAAAAG
    ACCACATCCA CTACATTAGT AGAAGCAGAG GTTCCAGAAC TTTCACAAAC TACCCAAACA
    CAACAGACTG TCCATGTAAA GCAGAAGATT ACACATGATG AGGCACCGGC ACAGCCTAAA
    ATCCCCAAAC CTACACCACC TCCCAAGTGC ATCAAAGCTC CAGAAGTCCT GTCGGCACCT
    TCACGAGTTC CTCCAGCTCC ATCAAAGCCT GTACCCCTGA TCACCCCCAA ACCCTACAGC
    CAGCCCAAAT TCAGCCAAGG CTTCCGCAAA TCGTTGAAGA GTGATGGGCT GATACGTGTG
    AACGGAGACC ACAATCAGCA GGTGATAAGA GAGAAGGCTA ATAGAGATCC TCTGCAGAGC
    CCTGAAGTCA ACTTCTCTTC CTCACCTCCA AAGCCAGAAA TTCAAAGTCA ACAGAATGAG
    GATCCAATCG TGTTAGAAAC CACAGACCAC CAGCAAGACC TACAGACACC TAAACCTGAC
    CTGGAGAAAG AAGTCATAAA GAAGGAGGAA GAAAGTCTGC TTAGTCCTCA GAAAGAGATC
    AGCAAAGAAG ACACCTCAAC TACACTTTCA GTGTCAGAAC CAGCAGGAAA GCAGCCAGTG
    CCTGACACAG TGATGAAGCG CAACTTTGTT GTCACAACAA CCATAGTGAC AGAACTCACT
    CAGACGCACC CCGTCCCACT AGAGGACACG TCCAGCACAG AGCAGCCTGA TACAGTCCAA
    AATACATGTG ATCTGAGCTC AACTGCAGAG TCCTGTAAAG CTGATCAGTC TTCATACTCG
    ATCTCGTCGA CAGAGGGGAT CGACTACTCC ATTGAATGTG AGAAATATCA GCGCGAGCAG
    GAGAAACTGA AGGAGGAATG GGAGAAAGCC CAGAGAGAAG TAGAAGAGGA AGAAAGGAGA
    CACCATGAAG AGGAGAGACG AATTCTGGAG GAGACAGTGA CCCCGCTGAC CCCTCGCACC
    TCAGCGTTAT CATCCAGCTG CGGGACAGAG GCTCCGTCCC TCACACAGCC CTCAGCTCCA
    CATGAAACCA TTGTCCTCTC ATTGGCCGAC TGGGAAAGGA AACAGGAAAT GCTGGAGAAA
    CAGGCACAGA CGGGCCAGAG TAACGGATTG ACACACAGTC CTGATCCAGT AATTGCAGCA
    GCTCAGCAGA ACGGACAAAG AGCTGAAGCT CAGGAGAGCC AGATCACAGC TACACCACAA
    CTGCAGTTCA TACAGGATGG CTCGTGGAGC TGCAAGTCTA AAGGCAGGCA GGAGGATCTG
    AAGAAAACCG CCTCGCTGGA TCGTAAACAG AGTCCAGCCC CAAGCCAGAC CACAGGGATG
    AGGAGGACTG GGTCATGTGA GAATGTTTTA GGAACACACC CATCAAAATC ACCCACACCA
    TCACAAGACA CGCCACCTCC ATCACCCAGC AGGTCAGTCA GTGGAAAGAA GCTTTGCTCC
    AGTTGCGCTC ATCCACTGGG TAAAGGAGCA GCAATGATCA TTGAAACCTT GGGGCTTTAC
    TTCCACATCC AGTGTTTTAA GTGTGGCGTA TGTAAAGGAT TACTTGGAGA CACAAGTGCT
    GGAACTGACG TCAGGATTCG AAATGGACTT CTTAATTGTC ACGAGTGCTA CATAAAATCA
    AGGGCTGCTG GTCAGCCAAC AACGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53713
    Clone ID Related Accession (Same CDS sequence) XM_017355100.1
    Accession Version XM_017355100.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product LIM and calponin homology domains-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTGGAGACGT AAACCCTGAG CCTGCTCCTA TCATGCCTCC TGAACCTCCA
    AACCCGTTCA TGTTTGACCT TAAACGGCCT TTGACCTCCA CCGAGCGCAG GGTGCTGGAG
    GATGAACTGG TTGAGAAAGC AAAGGATGAG AAGGATGATG ATGAAGAAGA CGAAGCCGAA
    GAAAGGCAGC CGGATATTGA AAAGGATGAT ATGCTAGCTC GCAGAACAAG AGCATTTAAG
    AAGACAGCGG GTGGGACTTC GTTTAACCAG TTCCTCCCGC TGCCGGGGTC CAAGAGAGAG
    GGAACTGTAG GTGGTTCTTT AGCCCAGAAA GGAGGTGTAC TGATAGGCTC GAGTGGAGAC
    AGAGACCTGC ACTACCTGGA GAGAAATATA AGGACTAAGA GAACCAAGAT TGGACAGAGA
    TTTGAACCAG TCGATCAAGT AGCAAAGACA CCGGTTGCTG TAGTCACCCA GCAGAGCCCT
    GATATTGTGC GCTCTACTGC ATCTAACACT GATCGCTCTG CAAGTTGCGA TTATGATGAT
    GATGATGATG ATGAATATGT GGATGACGGT CAATCTCCAT ATGTTGAGAA GGATGATATG
    CTTGCGCGAA GAACTGGATC CTATCAAAAG CCAAACGCAG GGGCAGGACA GTCTTTTAAT
    GCATTTCTGC CCAAACCTGG TGCTGTCAAA CATAAAATCA CCCCTGTCAG CGGATCACAG
    TCTTGCGTTA AGCCAAGAGA GCATGAGACA GCCCCATCAC AGGACAGCTT CACCCTTAGC
    TCTGTGGCCC CGCCCCCAAG AGATGCTGAG GACATGCCCA GGAAGTGGCC TCAGATGATG
    GACAGGCAGG AGGCGGGCTC TGGGGGACAG GAAAGTCTTA TTAAACATGA GACTATTACC
    AAAAGCCTGT CCCCTATGCC TGAGCAACCG TCCCGTCGGC CCCTTTGGCA GGAAGATGAT
    GATGATCTAC CCCCAATATT CAGAGCTACT AGTGTTTCTG ATGAAGCGGA GAGCGTGAGT
    TTGATTGACA TGCGTGATGA AGAGGATGAG CTTGCATACC TTCGGCCGCA CAGCCAATCA
    CGACACGAGC ACCTACACAA CCAGTACAAC AAAGTGAAGG AGGAGGAGGA CCAGTGGCAG
    GACGATTTGG CTCGTTGGAA GAACCGGAGG CGAAGCGCTT CTCAAGATCT GATCAAAAAG
    GAGGAGGAGA GGAAGATGAT GGAGAAGCTA ATGACAACGG ATGGAGGACA CGGACATCGC
    AAGAAGAGCA TCAAAACATA CAAAGAAATT GTAGAGGAGA AAGAGAAAAG GGAACAGGAG
    CTGCATGAAC AATACTGTAA GGCCTCCAGT CCAGAAGAGA AAGCAGCTGT GCTTCAGCGA
    TATGCTCTCC GTTTCACCAT CAGCGATGCC ATACTGGAGA AACTCCAGCT CCCCAAACTG
    CCTTCTGCCG CCTCACCGAT GCACACGTCC CCCCTCCATC AGCCCGAGCA AAAGACCACA
    TCCACTACAT TAGTAGAAGC AGAGGTTCCA GAACTTTCAC AAACTACCCA AACACAACAG
    ACTGTCCATG TAAAGCAGAA GATTACACAT GATGAGGCAC CGGCACAGCC TAAAATCCCC
    AAACCTACAC CACCTCCCAA GTGCATCAAA GCTCCAGAAG TCCTGTCGGC ACCTTCACGA
    GTTCCTCCAG CTCCATCAAA GCCTGTACCC CTGATCACCC CCAAACCCTA CAGCCAGCCC
    AAATTCAGCC AAGGCTTCCG CAAATCGTTG AAGAGTGATG GGCTGATACG TGTGAACGGA
    GACCACAATC AGCAGGTGAT AAGAGAGAAG GCTAATAGAG ATCCTCTGCA GAGCCCTGAA
    GTCAACTTCT CTTCCTCACC TCCAAAGCCA GAAATTCAAA GTCAACAGAA TGAGGATCCA
    ATCGTGTTAG AAACCACAGA CCACCAGCAA GACCTACAGA CACCTAAACC TGACCTGGAG
    AAAGAAGTCA TAAAGAAGGA GGAAGAAAGT CTGCTTAGTC CTCAGAAAGA GATCAGCAAA
    GAAGACACCT CAACTACACT TTCAGTGTCA GAACCAGCAG GAAAGCAGCC AGTGCCTGAC
    ACAGTGATGA AGCGCAACTT TGTTGTCACA ACAACCATAG TGACAGAACT CACTCAGACG
    CACCCCGTCC CACTAGAGGA CACGTCCAGC ACAGAGCAGC CTGATACAGT CCAAAATACA
    TGTGATCTGA GCTCAACTGC AGAGTCCTGT AAAGCTGATC AGTCTTCATA CTCGATCTCG
    TCGACAGAGG GGATCGACTA CTCCATTGAA TGTATTGAAA CCCCAATGTT GAACCTTGCA
    AAAAGGGTTG ACCACTGGAC GTGGGATCCC AATGAGGAGC GCAGGCGGCA GGAGAGGTGG
    CAGCAGGAGC AGGAGCGTCT CCTGCAGGAG AAATATCAGC GCGAGCAGGA GAAACTGAAG
    GAGGAATGGG AGAAAGCCCA GAGAGAAGTA GAAGAGGAAG AAAGGAGACA CCATGAAGAG
    GAGAGACGAA TTCTGGAGGA GACAGTGACC CCGCTGACCC CTCGCACCTC AGCGTTATCA
    TCCAGCTGCG GGACAGAGGC TCCGTCCCTC ACACAGCCCT CAGCTCCACA TGAAACCATT
    GTCCTCTCAT TGGCCGACTG GGAAAGGAAA CAGGAAATGC TGGAGAAACA GGCACAGACG
    GGCCAGAGTA ACGGATTGAC ACACAGTCCT GATCCAGTAA TTGCAGCAGC TCAGCAGAAC
    GGACAAAGAG CTGAAGCTCA GGAGAGCCAG ATCACAGCTA CACCACAACT GCAGTTCATA
    CAGGATGGCT CGTGGAGCTG CAAGTCTAAA GGCAGGCAGG AGGATCTGAA GAAAACCGCC
    TCGCTGGATC GTAAACAGAG TCCAGCCCCA AGCCAGACCA CAGGGATGAG GAGGACTGGG
    TCATGTGAGA ATGTTTTAGG AACACACCCA TCAAAATCAC CCACACCATC ACAAGACACG
    CCACCTCCAT CACCCAGCAG GTCAGTCAGT GGAAAGAAGC TTTGCTCCAG TTGCGCTCAT
    CCACTGGGTA AAGGAGCAGC AATGATCATT GAAACCTTGG GGCTTTACTT CCACATCCAG
    TGTTTTAAGT GTGGCGTATG TAAAGGATTA CTTGGAGACA CAAGTGCTGG AACTGACGTC
    AGGATTCGAA ATGGACTTCT TAATTGTCAC GAGTGCTACA TAAAATCAAG GGCTGCTGGT
    CAGCCAACAA CGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210589.1
    CDS373..5070
    Translation

    Target ORF information:

    RefSeq Version XM_017355100.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio LIM and calponin homology domains 1a (limch1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCAGTT CTGGAGACGA CTCGGCTTCA AACGCCCAGC TGGATCAGCC TGCAGAAGCG 
    ACCCCCGAAT CAGCCTTTCA AGAAGCTCAG AAATGGATCG AGGCAGTGAC AGGAAGAAGC
    TTCGGGGATA AGGACTTCAG AAGTGGACTA GAGAATGGGA TTTTGCTTTG CGAGCTGCTG
    AGTTCAATCA AGCCAGGTCT TGTGAAAAAG ATAAATAGAC TTCCAACACC CATCGCAGGA
    CTGGATAATC TGACAATGTT TCTGAGAGGA TGTGAAGAGG TGGGATTGAA GGGGTCACAG
    CTGTTCGATC CTGGAGACCT GCAAGATACA TCTATACGAG CAAACTTAAC GGGCTCTGAC
    TGCAGTAGAA AACTAAAGAA TGTGCTCATA ACCATATACT GGCTTGGGAA AGCTGCTAAC
    AGCTGTGCGA CATATAACGG CCCCACACTC GACCTGAAAG AGTTTGAGGG ACTGCTTTCC
    ATGATGCGAA AGGAGTGTGT AAATGACGAG TCAGACTCTC CTAAGCGCAG CATACGGGAC
    AGTGGTTACA TTGACAGCTG GGAATCGGAG CGCTCTGATT CGCTGTCTCC TCCAAGACAC
    GGCCGTGATG ATTCGTTCGA CAGTCTCGAC TCGTTTGGCT CACGATCACA GAACACACCA
    TCGCCGGACA CAGTCATTCG CTGCAACAGT GACGACTCTG AAGCTGATAG GAAGTTGCCA
    GATGTGCGTA AGGATGACAT GTCTGCCCGT CGTGTATCCT ACAAAGAGCC TCGTGCCGCT
    TTACCCTTCA ACCAGTATCT GCCGAACAAG AGCAACCAGA CTAACTACCT TCCTGCCCCA
    CTGAGGAAGA GGAGGAGCGA CAGGGAGGAA GACCGGAGGA GTGTGAGCAA CTCGACATCT
    CCAGTGGGAG GAGAACGACC CCTCAGACAA GAGTGTGTAG AAGTGTTAAA AGAAGGGGCT
    GTCGTGTCTC AAAAAAAAGT CCAGAAACAT TTCACCTGGA TGGTTGAGAA AGGGGCGGAG
    CTTGATGAGG CGGAGCTTAA GAAAATGAGG AAGCTGGAAA AAGCGGGAAT TAAAGTGTTA
    CCCGCCGCTG TTCGATACAA CAGTCCAATC GTGAGTCCTG CTGAAGCCAG CAAACCGAAA
    TCCCCTTCAC CCGACATCAT CCTCCGGCGT GACAATGACT TCATGCGCGC TGCCGTTCCA
    CATCAGTGGG ACTCCGATGA GGAAGAGGAA GATGAAGAAG CAGAGCAGAA GGTTCCAGAT
    GTTCAAAAGG ACGATCTTGC TTCTCGGAGA GCACGCATGA ATCAGTTCAA ACCAAGTGTG
    GCACATCACT TTCTGCCAAG CTCCTGCAGC TGGAAGGACC GAGAGAAATG GGAGGGAATC
    AGGATGGCAT CCCAACATGC CGTGTTGGAG AGGATGGAGA AGATGGAACA GGAGAAACTG
    GAGAAAAGGA GTGGAGACGT AAACCCTGAG CCTGCTCCTA TCATGCCTCC TGAACCTCCA
    AACCCGTTCA TGTTTGACCT TAAACGGCCT TTGACCTCCA CCGAGCGCAG GGTGCTGGAG
    GATGAACTGG TTGAGAAAGC AAAGGATGAG AAGGATGATG ATGAAGAAGA CGAAGCCGAA
    GAAAGGCAGC CGGATATTGA AAAGGATGAT ATGCTAGCTC GCAGAACAAG AGCATTTAAG
    AAGACAGCGG GTGGGACTTC GTTTAACCAG TTCCTCCCGC TGCCGGGGTC CAAGAGAGAG
    GGAACTGTAG GTGGTTCTTT AGCCCAGAAA GGAGGTGTAC TGATAGGCTC GAGTGGAGAC
    AGAGACCTGC ACTACCTGGA GAGAAATATA AGGACTAAGA GAACCAAGAT TGGACAGAGA
    TTTGAACCAG TCGATCAAGT AGCAAAGACA CCGGTTGCTG TAGTCACCCA GCAGAGCCCT
    GATATTGTGC GCTCTACTGC ATCTAACACT GATCGCTCTG CAAGTTGCGA TTATGATGAT
    GATGATGATG ATGAATATGT GGATGACGGT CAATCTCCAT ATGTTGAGAA GGATGATATG
    CTTGCGCGAA GAACTGGATC CTATCAAAAG CCAAACGCAG GGGCAGGACA GTCTTTTAAT
    GCATTTCTGC CCAAACCTGG TGCTGTCAAA CATAAAATCA CCCCTGTCAG CGGATCACAG
    TCTTGCGTTA AGCCAAGAGA GCATGAGACA GCCCCATCAC AGGACAGCTT CACCCTTAGC
    TCTGTGGCCC CGCCCCCAAG AGATGCTGAG GACATGCCCA GGAAGTGGCC TCAGATGATG
    GACAGGCAGG AGGCGGGCTC TGGGGGACAG GAAAGTCTTA TTAAACATGA GACTATTACC
    AAAAGCCTGT CCCCTATGCC TGAGCAACCG TCCCGTCGGC CCCTTTGGCA GGAAGATGAT
    GATGATCTAC CCCCAATATT CAGAGCTACT AGTGTTTCTG ATGAAGCGGA GAGCGTGAGT
    TTGATTGACA TGCGTGATGA AGAGGATGAG CTTGCATACC TTCGGCCGCA CAGCCAATCA
    CGACACGAGC ACCTACACAA CCAGTACAAC AAAGTGAAGG AGGAGGAGGA CCAGTGGCAG
    GACGATTTGG CTCGTTGGAA GAACCGGAGG CGAAGCGCTT CTCAAGATCT GATCAAAAAG
    GAGGAGGAGA GGAAGATGAT GGAGAAGCTA ATGACAACGG ATGGAGGACA CGGACATCGC
    AAGAAGAGCA TCAAAACATA CAAAGAAATT GTAGAGGAGA AAGAGAAAAG GGAACAGGAG
    CTGCATGAAC AATACTGTAA GGCCTCCAGT CCAGAAGAGA AAGCAGCTGT GCTTCAGCGA
    TATGCTCTCC GTTTCACCAT CAGCGATGCC ATACTGGAGA AACTCCAGCT CCCCAAACTG
    CCTTCTGCCG CCTCACCGAT GCACACGTCC CCCCTCCATC AGCCCGAGCA AAAGACCACA
    TCCACTACAT TAGTAGAAGC AGAGGTTCCA GAACTTTCAC AAACTACCCA AACACAACAG
    ACTGTCCATG TAAAGCAGAA GATTACACAT GATGAGGCAC CGGCACAGCC TAAAATCCCC
    AAACCTACAC CACCTCCCAA GTGCATCAAA GCTCCAGAAG TCCTGTCGGC ACCTTCACGA
    GTTCCTCCAG CTCCATCAAA GCCTGTACCC CTGATCACCC CCAAACCCTA CAGCCAGCCC
    AAATTCAGCC AAGGCTTCCG CAAATCGTTG AAGAGTGATG GGCTGATACG TGTGAACGGA
    GACCACAATC AGCAGGTGAT AAGAGAGAAG GCTAATAGAG ATCCTCTGCA GAGCCCTGAA
    GTCAACTTCT CTTCCTCACC TCCAAAGCCA GAAATTCAAA GTCAACAGAA TGAGGATCCA
    ATCGTGTTAG AAACCACAGA CCACCAGCAA GACCTACAGA CACCTAAACC TGACCTGGAG
    AAAGAAGTCA TAAAGAAGGA GGAAGAAAGT CTGCTTAGTC CTCAGAAAGA GATCAGCAAA
    GAAGACACCT CAACTACACT TTCAGTGTCA GAACCAGCAG GAAAGCAGCC AGTGCCTGAC
    ACAGTGATGA AGCGCAACTT TGTTGTCACA ACAACCATAG TGACAGAACT CACTCAGACG
    CACCCCGTCC CACTAGAGGA CACGTCCAGC ACAGAGCAGC CTGATACAGT CCAAAATACA
    TGTGATCTGA GCTCAACTGC AGAGTCCTGT AAAGCTGATC AGTCTTCATA CTCGATCTCG
    TCGACAGAGG GGATCGACTA CTCCATTGAA TGTATTGAAA CCCCAATGTT GAACCTTGCA
    AAAAGGGTTG ACCACTGGAC GTGGGATCCC AATGAGGAGC GCAGGCGGCA GGAGAGGTGG
    CAGCAGGAGC AGGAGCGTCT CCTGCAGGAG AAATATCAGC GCGAGCAGGA GAAACTGAAG
    GAGGAATGGG AGAAAGCCCA GAGAGAAGTA GAAGAGGAAG AAAGGAGACA CCATGAAGAG
    GAGAGACGAA TTCTGGAGGA GACAGTGACC CCGCTGACCC CTCGCACCTC AGCGTTATCA
    TCCAGCTGCG GGACAGAGGC TCCGTCCCTC ACACAGCCCT CAGCTCCACA TGAAACCATT
    GTCCTCTCAT TGGCCGACTG GGAAAGGAAA CAGGAAATGC TGGAGAAACA GGCACAGACG
    GGCCAGAGTA ACGGATTGAC ACACAGTCCT GATCCAGTAA TTGCAGCAGC TCAGCAGAAC
    GGACAAAGAG CTGAAGCTCA GGAGAGCCAG ATCACAGCTA CACCACAACT GCAGTTCATA
    CAGGATGGCT CGTGGAGCTG CAAGTCTAAA GGCAGGCAGG AGGATCTGAA GAAAACCGCC
    TCGCTGGATC GTAAACAGAG TCCAGCCCCA AGCCAGACCA CAGGGATGAG GAGGACTGGG
    TCATGTGAGA ATGTTTTAGG AACACACCCA TCAAAATCAC CCACACCATC ACAAGACACG
    CCACCTCCAT CACCCAGCAG GTCAGTCAGT GGAAAGAAGC TTTGCTCCAG TTGCGCTCAT
    CCACTGGGTA AAGGAGCAGC AATGATCATT GAAACCTTGG GGCTTTACTT CCACATCCAG
    TGTTTTAAGT GTGGCGTATG TAAAGGATTA CTTGGAGACA CAAGTGCTGG AACTGACGTC
    AGGATTCGAA ATGGACTTCT TAATTGTCAC GAGTGCTACA TAAAATCAAG GGCTGCTGGT
    CAGCCAACAA CGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.