spag9a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021470820.1	XP_021326495.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
XM_021470833.1	XP_021326508.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
XM_017354364.1	XP_017209853.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
XM_017354369.1	XP_017209858.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
XM_017354375.1	XP_017209864.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X8
XM_017354367.1	XP_017209856.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
XM_017354368.1	XP_017209857.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
XM_017354371.1	XP_017209860.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
XM_017354372.1	XP_017209861.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
XM_021470835.1	XP_021326510.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
XM_017354369.2	XP_017209858.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
XM_017354374.2	XP_017209863.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
XM_021470831.1	XP_021326506.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
XM_021470832.1	XP_021326507.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
XM_017354374.1	XP_017209863.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
XM_005163891.3	XP_005163948.2	C-Jun-amino-terminal kinase-interacting protein 4 isoform X9
XM_017354377.1	XP_017209866.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X10
NM_001320288.1	NP_001307217.1	C-Jun-amino-terminal kinase-interacting protein 4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa51594	XM_005163891.3 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa58063	XM_017354377.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa60513	NM_001320288.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa141583	XM_021470820.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa141584	XM_021470831.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa141585	XM_021470832.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa141586	XM_021470833.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa141587	XM_021470835.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58055	XM_017354364.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58056	XM_017354367.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58057	XM_017354368.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58058	XM_017354369.2 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58059	XM_017354371.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58060	XM_017354372.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58061	XM_017354374.2 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58062	XM_017354375.1 Latest version!	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
ODa58058	XM_017354369.1	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa58061	XM_017354374.1	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

spag9a ( XM_005163891.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163948.2 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa51594
Clone ID Related Accession (Same CDS sequence)	XM_005163891.3
Accession Version	XM_005163891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGA AGGGGCCGAT CTGTTGGTGG AGTACTCTGC TGTCCATTCG TGTTTCACTC 
TCCATGTGTC TGTCTCTCTG TCGAACACAC GCAGTGCATG CGCTTTCACA CCCGAGTCTG 
TCCGGCATGA GTCGTGAGGT GGAGAATCTC ATTCAGGAAA ATGTGCAGTT GCTTGAGACA 
AAAAATGCAT TGAATGTGGT AAAGAATGAT CTGATCGCTC GCGTGGACGA GCTCTCCTGC 
GAGAAGGAGG TCCTTCAGGG GGAACTGGAT GCGGTTTTGC AGGCCAAAGC CAAACTAGAG 
GAGAAATACA AGGAGCTGGA GGAGGAGCTC AAAAAAATAA AAACCGAGAC AGAGGAGGCC 
AAACAGAACA ATAATAAAGA AGAAGATGAG AGTGATGTGC CCACTGCTCA GAGACGGAGG 
TTCACGCGGG TGGAAATGGC TCGAGTGCTG ATGGAGAGAA ACCAGTACAA GGAGAGACTG 
ATGGAGCTGC AGGAGGCCGT CCGGTGGACC GAAATGATTC GAGCTTCAAG AGAAAATTCA 
GAAGTCGCAG AAAAGAAGAG GTCAAGCATC AGGCAATTCT TCAGTCGCCT CTTCAGTTCT 
TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC AGAGCTTTAC CGTCCAACGT CAAGTACAAC 
CCACCCAGCA TCATGAAGAA GAGCAACACC TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA 
GCTTTCGACT TCCTCAAAGA AGAGGTGGAC TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG 
AAGCGGGCTC AGTATCAGCA TGTGAAAGCA CACATGCAGA AAGAGGACGG CAGGGTGACC 
GCACATGGCT GGAGTCTGCC GGGAAAATAC AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC 
AAAATAAACC TGCCGGTTCC TGTGTGTCTC AAGCCACTGG AGCAGAAAGA TGCTTCTATG 
AAGCTGCTGT GTGCCACTGG AGTGAATCTC TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT 
GCTGAAGACT CCACAGTAGA GACAGACTCA GCCAAACAGA GGAAAGGCTC TCAGAGCAGC 
CTAGATCAGC TGGAGCACGA TCCTAAGGAG CGGGAGCAGA CGCTGCATGT GGAAATGTCC 
AGTCGCGTCT GGATCTGCAC GAGCACTCAC TCCTCTACTA AGGTGATGGT CATAGATGTG 
ACGCGACCCG ACGACCTCCT GGACAGCTTC TACGTCTGTA ACTCATGCGT GCTGTGTATC 
GCCAGCATAC CAGGAGTGTT GGAGACAGAT TATCCTGCGG GGGAGGAGAT TCCTCAGGAT 
CTGGAGGCGG GACCAGACGC TCTATTGGCC AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG 
TTGAGTGACG TGTCGGCGGT TGGCTGTGGT GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG 
GGCAGCACAG GAGCCCACGA ACAGTCTGCT GAACAGAGTC CCGCCAGAGG AAGTGATCTC 
TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT GCCGCTGAGG AGGCTATGGA GGCCACTGAG 
GAGGACCAGG AGAGTCCGGG ACAGGACAGC AGTCAGCCGG GTATCTACAC GGAGCATGTG 
TTTACAGACC CGCTGGGACC CGAGTCTTCT GGGGCGTCAG CAGGCCATGA TAGCCAGAGA 
GAGTCCGATC GGGACAGGGA TGACACGAGC TCAGCAGGGG GCAAGACGGA TCTGGCAAAA 
GAAGAAACGC AGCGGATGAG CAGTGCACTG CCCACCATGT GGCTGGGGGC TCAGAACGGC 
TGTTTATATG TGCACTCATC TGTTGCTCGA TGGAGGAAGT GTCTTCATGC TGTCAAGCTA 
AAGGATTCCA TCTTGAACAT TGTACATGTG AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC 
GGGACACTGG CCATCTTCCA TAGAGGTCTT GACAGCCAGT GGGATTTAAC CAACTACCAT 
CTTCTGGACC TGGGCCGGCC GCATCACTCA ATCCGCTGCA TGACTGTGGT TCACGATAAA 
GTGTGGTGCG GCTACAGGAA CAAGATCTAC ATCATTCAGC CCAAAGCTAT GAAAATCGAG 
AGGTCTTTTG ATGCCCATCC GCGTAAGGAG AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT 
GACGGGATCT GGGTGTCCAT CCGGCTGGAC TCAACGCTTC GCCTATTCCA TGCTCACACC 
TTCCAGCATC TGCAGGACGT AGACATTGAG CCTTACGTCA GCAAAATGTT AGGAACTGGA 
AAACTGGGCT TCTCTTTTGT GAGAATCACT GCTCTGATGG TTTCTTGTAA TCGGCTGTGG 
GTGGGAACAG GAAATGGTGT CATCATCTCC ATCCCTCTGT CAGACTCTGT AGGTCAGTGT 
GGATATCTAG GTCAGAGGGC AGCTAATAAG ACGGTGGGAG CGATTGCCTC TCGTCCTGGT 
GGAGCAGTGC GTGTATACAG CGAGGACGCT GTGGACGGTG GGGTGACCGG CTCATACGTC 
CCGTACTGCT CCATGGCACA TGCTCAGCTC TGCTTCCATG GCCACAGGGA CGCTGTTAAA 
TTTTTCGCTA CAGTTCCAGG TCAGGTTATT GTGCCTGGAG CTGAGGGTGT GACTGAGAGC 
TCTGAGTCTT CAGATGGAAA GAGCATGTTG ATCATGAGTG GAGGAGAGGG ATACATCGAC 
TTCAGAGTGG GTGATGAGGA TGGAGGATTG GGACGAGATG AAGATGAAGA ATCAGCTGAA 
CCCACACTGA AACTCCAGTC GGCTCCTGCC AAACCTGAGC GCAGTTATGT TATAGTCTGG 
CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163948.2
CDS	90..2930
Translation	MWMKGPICWWSTLLSIRVSLSMCLSLCRTHAVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_005163891.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163891.3

ORF Insert Sequence:

ATGTGGATGA AGGGGCCGAT CTGTTGGTGG AGTACTCTGC TGTCCATTCG TGTTTCACTC 
TCCATGTGTC TGTCTCTCTG TCGAACACAC GCAGTGCATG CGCTTTCACA CCCGAGTCTG 
TCCGGCATGA GTCGTGAGGT GGAGAATCTC ATTCAGGAAA ATGTGCAGTT GCTTGAGACA 
AAAAATGCAT TGAATGTGGT AAAGAATGAT CTGATCGCTC GCGTGGACGA GCTCTCCTGC 
GAGAAGGAGG TCCTTCAGGG GGAACTGGAT GCGGTTTTGC AGGCCAAAGC CAAACTAGAG 
GAGAAATACA AGGAGCTGGA GGAGGAGCTC AAAAAAATAA AAACCGAGAC AGAGGAGGCC 
AAACAGAACA ATAATAAAGA AGAAGATGAG AGTGATGTGC CCACTGCTCA GAGACGGAGG 
TTCACGCGGG TGGAAATGGC TCGAGTGCTG ATGGAGAGAA ACCAGTACAA GGAGAGACTG 
ATGGAGCTGC AGGAGGCCGT CCGGTGGACC GAAATGATTC GAGCTTCAAG AGAAAATTCA 
GAAGTCGCAG AAAAGAAGAG GTCAAGCATC AGGCAATTCT TCAGTCGCCT CTTCAGTTCT 
TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC AGAGCTTTAC CGTCCAACGT CAAGTACAAC 
CCACCCAGCA TCATGAAGAA GAGCAACACC TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA 
GCTTTCGACT TCCTCAAAGA AGAGGTGGAC TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG 
AAGCGGGCTC AGTATCAGCA TGTGAAAGCA CACATGCAGA AAGAGGACGG CAGGGTGACC 
GCACATGGCT GGAGTCTGCC GGGAAAATAC AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC 
AAAATAAACC TGCCGGTTCC TGTGTGTCTC AAGCCACTGG AGCAGAAAGA TGCTTCTATG 
AAGCTGCTGT GTGCCACTGG AGTGAATCTC TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT 
GCTGAAGACT CCACAGTAGA GACAGACTCA GCCAAACAGA GGAAAGGCTC TCAGAGCAGC 
CTAGATCAGC TGGAGCACGA TCCTAAGGAG CGGGAGCAGA CGCTGCATGT GGAAATGTCC 
AGTCGCGTCT GGATCTGCAC GAGCACTCAC TCCTCTACTA AGGTGATGGT CATAGATGTG 
ACGCGACCCG ACGACCTCCT GGACAGCTTC TACGTCTGTA ACTCATGCGT GCTGTGTATC 
GCCAGCATAC CAGGAGTGTT GGAGACAGAT TATCCTGCGG GGGAGGAGAT TCCTCAGGAT 
CTGGAGGCGG GACCAGACGC TCTATTGGCC AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG 
TTGAGTGACG TGTCGGCGGT TGGCTGTGGT GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG 
GGCAGCACAG GAGCCCACGA ACAGTCTGCT GAACAGAGTC CCGCCAGAGG AAGTGATCTC 
TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT GCCGCTGAGG AGGCTATGGA GGCCACTGAG 
GAGGACCAGG AGAGTCCGGG ACAGGACAGC AGTCAGCCGG GTATCTACAC GGAGCATGTG 
TTTACAGACC CGCTGGGACC CGAGTCTTCT GGGGCGTCAG CAGGCCATGA TAGCCAGAGA 
GAGTCCGATC GGGACAGGGA TGACACGAGC TCAGCAGGGG GCAAGACGGA TCTGGCAAAA 
GAAGAAACGC AGCGGATGAG CAGTGCACTG CCCACCATGT GGCTGGGGGC TCAGAACGGC 
TGTTTATATG TGCACTCATC TGTTGCTCGA TGGAGGAAGT GTCTTCATGC TGTCAAGCTA 
AAGGATTCCA TCTTGAACAT TGTACATGTG AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC 
GGGACACTGG CCATCTTCCA TAGAGGTCTT GACAGCCAGT GGGATTTAAC CAACTACCAT 
CTTCTGGACC TGGGCCGGCC GCATCACTCA ATCCGCTGCA TGACTGTGGT TCACGATAAA 
GTGTGGTGCG GCTACAGGAA CAAGATCTAC ATCATTCAGC CCAAAGCTAT GAAAATCGAG 
AGGTCTTTTG ATGCCCATCC GCGTAAGGAG AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT 
GACGGGATCT GGGTGTCCAT CCGGCTGGAC TCAACGCTTC GCCTATTCCA TGCTCACACC 
TTCCAGCATC TGCAGGACGT AGACATTGAG CCTTACGTCA GCAAAATGTT AGGAACTGGA 
AAACTGGGCT TCTCTTTTGT GAGAATCACT GCTCTGATGG TTTCTTGTAA TCGGCTGTGG 
GTGGGAACAG GAAATGGTGT CATCATCTCC ATCCCTCTGT CAGACTCTGT AGGTCAGTGT 
GGATATCTAG GTCAGAGGGC AGCTAATAAG ACGGTGGGAG CGATTGCCTC TCGTCCTGGT 
GGAGCAGTGC GTGTATACAG CGAGGACGCT GTGGACGGTG GGGTGACCGG CTCATACGTC 
CCGTACTGCT CCATGGCACA TGCTCAGCTC TGCTTCCATG GCCACAGGGA CGCTGTTAAA 
TTTTTCGCTA CAGTTCCAGG TCAGGTTATT GTGCCTGGAG CTGAGGGTGT GACTGAGAGC 
TCTGAGTCTT CAGATGGAAA GAGCATGTTG ATCATGAGTG GAGGAGAGGG ATACATCGAC 
TTCAGAGTGG GTGATGAGGA TGGAGGATTG GGACGAGATG AAGATGAAGA ATCAGCTGAA 
CCCACACTGA AACTCCAGTC GGCTCCTGCC AAACCTGAGC GCAGTTATGT TATAGTCTGG 
CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354377.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209866.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa58063
Clone ID Related Accession (Same CDS sequence)	XM_017354377.1
Accession Version	XM_017354377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGA AGGGGCCGAT CTGTTGGCTG TCCATTCGTG TTTCACTCTC CATGTGTCTG 
TCTCTCTGTC GAACACACGC AGTGCATGCG CTTTCACACC CGAGTCTGTC CGGCATGAGT 
CGTGAGGTGG AGAATCTCAT TCAGGAAAAT GTGCAGTTGC TTGAGACAAA AAATGCATTG 
AATGTGGTAA AGAATGATCT GATCGCTCGC GTGGACGAGC TCTCCTGCGA GAAGGAGGTC 
CTTCAGGGGG AACTGGATGC GGTTTTGCAG GCCAAAGCCA AACTAGAGGA GAAATACAAG 
GAGCTGGAGG AGGAGCTCAA AAAAATAAAA ACCGAGACAG AGGAGGCCAA ACAGAACAAT 
AATAAAGAAG AAGATGAGAG TGATGTGCCC ACTGCTCAGA GACGGAGGTT CACGCGGGTG 
GAAATGGCTC GAGTGCTGAT GGAGAGAAAC CAGTACAAGG AGAGACTGAT GGAGCTGCAG 
GAGGCCGTCC GGTGGACCGA AATGATTCGA GCTTCAAGAG AAAATTCAGA AGTCGCAGAA 
AAGAAGAGGT CAAGCATCAG GCAATTCTTC AGTCGCCTCT TCAGTTCTTC CAGTTCTGCT 
GCGTCCAGCA GCTCGTCCAG AGCTTTACCG TCCAACGTCA AGTACAACCC ACCCAGCATC 
ATGAAGAAGA GCAACACCTT CTCCCAGTTC CCCAGAGAAA AGTCCAAAGC TTTCGACTTC 
CTCAAAGAAG AGGTGGACTC GAGTGGCTCT CTGTCCCGCC GGGAGCAGAA GCGGGCTCAG 
TATCAGCATG TGAAAGCACA CATGCAGAAA GAGGACGGCA GGGTGACCGC ACATGGCTGG 
AGTCTGCCGG GAAAATACAA GGTTGCCAAT GGTGGCCCTG TAGAGAACAA AATAAACCTG 
CCGGTTCCTG TGTGTCTCAA GCCACTGGAG CAGAAAGATG CTTCTATGAA GCTGCTGTGT 
GCCACTGGAG TGAATCTCTC TGGAGGACGC TTGTGTGACA GAGGCTCTGC TGAAGACTCC 
ACAGTAGAGA CAGACTCAGC CAAACAGAGG AAAGGCTCTC AGAGCAGCCT AGATCAGCTG 
GAGCACGATC CTAAGGAGCG GGAGCAGACG CTGCATGTGG AAATGTCCAG TCGCGTCTGG 
ATCTGCACGA GCACTCACTC CTCTACTAAG GTGATGGTCA TAGATGTGAC GCGACCCGAC 
GACCTCCTGG ACAGCTTCTA CGTCTGTAAC TCATGCGTGC TGTGTATCGC CAGCATACCA 
GGAGTGTTGG AGACAGATTA TCCTGCGGGG GAGGAGATTC CTCAGGATCT GGAGGCGGGA 
CCAGACGCTC TATTGGCCAG CAGCAGCTCT CAGGGCAGTG AGGGAGTGTT GAGTGACGTG 
TCGGCGGTTG GCTGTGGTGT TGAGGAGTCC AGTTTAGGCT CCCAAGCGGG CAGCACAGGA 
GCCCACGAAC AGTCTGCTGA ACAGAGTCCC GCCAGAGGAA GTGATCTCTC TAGAGCTCCC 
AGTGAAGATG GTCTCCCTGC CGCTGAGGAG GCTATGGAGG CCACTGAGGA GGACCAGGAG 
AGTCCGGGAC AGGACAGCAG TCAGCCGGGT ATCTACACGG AGCATGTGTT TACAGACCCG 
CTGGGACCCG AGTCTTCTGG GGCGTCAGCA GGCCATGATA GCCAGAGAGA GTCCGATCGG 
GACAGGGATG ACACGAGCTC AGCAGGGGGC AAGACGGATC TGGCAAAAGA AGAAACGCAG 
CGGATGAGCA GTGCACTGCC CACCATGTGG CTGGGGGCTC AGAACGGCTG TTTATATGTG 
CACTCATCTG TTGCTCGATG GAGGAAGTGT CTTCATGCTG TCAAGCTAAA GGATTCCATC 
TTGAACATTG TACATGTGAA AGGCCGTGTT TTGGTGGCTC TAGCTGACGG GACACTGGCC 
ATCTTCCATA GAGGTCTTGA CAGCCAGTGG GATTTAACCA ACTACCATCT TCTGGACCTG 
GGCCGGCCGC ATCACTCAAT CCGCTGCATG ACTGTGGTTC ACGATAAAGT GTGGTGCGGC 
TACAGGAACA AGATCTACAT CATTCAGCCC AAAGCTATGA AAATCGAGAG GTCTTTTGAT 
GCCCATCCGC GTAAGGAGAG TCAGGTGCGT CAGCTGGCCT GGGTGGGTGA CGGGATCTGG 
GTGTCCATCC GGCTGGACTC AACGCTTCGC CTATTCCATG CTCACACCTT CCAGCATCTG 
CAGGACGTAG ACATTGAGCC TTACGTCAGC AAAATGTTAG GAACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATCACTGC TCTGATGGTT TCTTGTAATC GGCTGTGGGT GGGAACAGGA 
AATGGTGTCA TCATCTCCAT CCCTCTGTCA GACTCTGTAG GTCAGTGTGG ATATCTAGGT 
CAGAGGGCAG CTAATAAGAC GGTGGGAGCG ATTGCCTCTC GTCCTGGTGG AGCAGTGCGT 
GTATACAGCG AGGACGCTGT GGACGGTGGG GTGACCGGCT CATACGTCCC GTACTGCTCC 
ATGGCACATG CTCAGCTCTG CTTCCATGGC CACAGGGACG CTGTTAAATT TTTCGCTACA 
GTTCCAGGTC AGGTTATTGT GCCTGGAGCT GAGGGTGTGA CTGAGAGCTC TGAGTCTTCA 
GATGGAAAGA GCATGTTGAT CATGAGTGGA GGAGAGGGAT ACATCGACTT CAGAGTGGGT 
GATGAGGATG GAGGATTGGG ACGAGATGAA GATGAAGAAT CAGCTGAACC CACACTGAAA 
CTCCAGTCGG CTCCTGCCAA ACCTGAGCGC AGTTATGTTA TAGTCTGGCA GGTTGCGAGT 
GACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209866.1
CDS	90..2918
Translation	MWMKGPICWLSIRVSLSMCLSLCRTHAVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354377.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354377.1

ORF Insert Sequence:

ATGTGGATGA AGGGGCCGAT CTGTTGGCTG TCCATTCGTG TTTCACTCTC CATGTGTCTG 
TCTCTCTGTC GAACACACGC AGTGCATGCG CTTTCACACC CGAGTCTGTC CGGCATGAGT 
CGTGAGGTGG AGAATCTCAT TCAGGAAAAT GTGCAGTTGC TTGAGACAAA AAATGCATTG 
AATGTGGTAA AGAATGATCT GATCGCTCGC GTGGACGAGC TCTCCTGCGA GAAGGAGGTC 
CTTCAGGGGG AACTGGATGC GGTTTTGCAG GCCAAAGCCA AACTAGAGGA GAAATACAAG 
GAGCTGGAGG AGGAGCTCAA AAAAATAAAA ACCGAGACAG AGGAGGCCAA ACAGAACAAT 
AATAAAGAAG AAGATGAGAG TGATGTGCCC ACTGCTCAGA GACGGAGGTT CACGCGGGTG 
GAAATGGCTC GAGTGCTGAT GGAGAGAAAC CAGTACAAGG AGAGACTGAT GGAGCTGCAG 
GAGGCCGTCC GGTGGACCGA AATGATTCGA GCTTCAAGAG AAAATTCAGA AGTCGCAGAA 
AAGAAGAGGT CAAGCATCAG GCAATTCTTC AGTCGCCTCT TCAGTTCTTC CAGTTCTGCT 
GCGTCCAGCA GCTCGTCCAG AGCTTTACCG TCCAACGTCA AGTACAACCC ACCCAGCATC 
ATGAAGAAGA GCAACACCTT CTCCCAGTTC CCCAGAGAAA AGTCCAAAGC TTTCGACTTC 
CTCAAAGAAG AGGTGGACTC GAGTGGCTCT CTGTCCCGCC GGGAGCAGAA GCGGGCTCAG 
TATCAGCATG TGAAAGCACA CATGCAGAAA GAGGACGGCA GGGTGACCGC ACATGGCTGG 
AGTCTGCCGG GAAAATACAA GGTTGCCAAT GGTGGCCCTG TAGAGAACAA AATAAACCTG 
CCGGTTCCTG TGTGTCTCAA GCCACTGGAG CAGAAAGATG CTTCTATGAA GCTGCTGTGT 
GCCACTGGAG TGAATCTCTC TGGAGGACGC TTGTGTGACA GAGGCTCTGC TGAAGACTCC 
ACAGTAGAGA CAGACTCAGC CAAACAGAGG AAAGGCTCTC AGAGCAGCCT AGATCAGCTG 
GAGCACGATC CTAAGGAGCG GGAGCAGACG CTGCATGTGG AAATGTCCAG TCGCGTCTGG 
ATCTGCACGA GCACTCACTC CTCTACTAAG GTGATGGTCA TAGATGTGAC GCGACCCGAC 
GACCTCCTGG ACAGCTTCTA CGTCTGTAAC TCATGCGTGC TGTGTATCGC CAGCATACCA 
GGAGTGTTGG AGACAGATTA TCCTGCGGGG GAGGAGATTC CTCAGGATCT GGAGGCGGGA 
CCAGACGCTC TATTGGCCAG CAGCAGCTCT CAGGGCAGTG AGGGAGTGTT GAGTGACGTG 
TCGGCGGTTG GCTGTGGTGT TGAGGAGTCC AGTTTAGGCT CCCAAGCGGG CAGCACAGGA 
GCCCACGAAC AGTCTGCTGA ACAGAGTCCC GCCAGAGGAA GTGATCTCTC TAGAGCTCCC 
AGTGAAGATG GTCTCCCTGC CGCTGAGGAG GCTATGGAGG CCACTGAGGA GGACCAGGAG 
AGTCCGGGAC AGGACAGCAG TCAGCCGGGT ATCTACACGG AGCATGTGTT TACAGACCCG 
CTGGGACCCG AGTCTTCTGG GGCGTCAGCA GGCCATGATA GCCAGAGAGA GTCCGATCGG 
GACAGGGATG ACACGAGCTC AGCAGGGGGC AAGACGGATC TGGCAAAAGA AGAAACGCAG 
CGGATGAGCA GTGCACTGCC CACCATGTGG CTGGGGGCTC AGAACGGCTG TTTATATGTG 
CACTCATCTG TTGCTCGATG GAGGAAGTGT CTTCATGCTG TCAAGCTAAA GGATTCCATC 
TTGAACATTG TACATGTGAA AGGCCGTGTT TTGGTGGCTC TAGCTGACGG GACACTGGCC 
ATCTTCCATA GAGGTCTTGA CAGCCAGTGG GATTTAACCA ACTACCATCT TCTGGACCTG 
GGCCGGCCGC ATCACTCAAT CCGCTGCATG ACTGTGGTTC ACGATAAAGT GTGGTGCGGC 
TACAGGAACA AGATCTACAT CATTCAGCCC AAAGCTATGA AAATCGAGAG GTCTTTTGAT 
GCCCATCCGC GTAAGGAGAG TCAGGTGCGT CAGCTGGCCT GGGTGGGTGA CGGGATCTGG 
GTGTCCATCC GGCTGGACTC AACGCTTCGC CTATTCCATG CTCACACCTT CCAGCATCTG 
CAGGACGTAG ACATTGAGCC TTACGTCAGC AAAATGTTAG GAACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATCACTGC TCTGATGGTT TCTTGTAATC GGCTGTGGGT GGGAACAGGA 
AATGGTGTCA TCATCTCCAT CCCTCTGTCA GACTCTGTAG GTCAGTGTGG ATATCTAGGT 
CAGAGGGCAG CTAATAAGAC GGTGGGAGCG ATTGCCTCTC GTCCTGGTGG AGCAGTGCGT 
GTATACAGCG AGGACGCTGT GGACGGTGGG GTGACCGGCT CATACGTCCC GTACTGCTCC 
ATGGCACATG CTCAGCTCTG CTTCCATGGC CACAGGGACG CTGTTAAATT TTTCGCTACA 
GTTCCAGGTC AGGTTATTGT GCCTGGAGCT GAGGGTGTGA CTGAGAGCTC TGAGTCTTCA 
GATGGAAAGA GCATGTTGAT CATGAGTGGA GGAGAGGGAT ACATCGACTT CAGAGTGGGT 
GATGAGGATG GAGGATTGGG ACGAGATGAA GATGAAGAAT CAGCTGAACC CACACTGAAA 
CTCCAGTCGG CTCCTGCCAA ACCTGAGCGC AGTTATGTTA TAGTCTGGCA GGTTGCGAGT 
GACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( NM_001320288.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001307217.1 Danio rerio sperm associated antigen 9a (spag9a), mRNA.

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CloneID	ODa60513
Clone ID Related Accession (Same CDS sequence)	NM_001320288.1
Accession Version	NM_001320288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR392027.16 and BX649434.7. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by partial transcript alignments, RNA-seq data and orthologous alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGGTTT GACTGATAAA GAGGAGAAGT CAGATGTTCA GGCCATTATT 
GAATCCACAC CGGAGCTGGA CATGGACCTT GGATATAAAG GCCCAAGTAC GCCGACCCGA 
GGGATTGAGA ACATGGCGTT TGACCGCAAC ACAGAATCCC TGTTTGAAGA AGTGTCATCG 
GCGGGCACGG GACTGATCGG AGATGTGGAT GAAGGGGCCG ATCTGTTGGT GGAGTACTCT 
GTGCATGCGC TTTCACACCC GAGTCTGTCC GGCATGAGTC GTGAGGTGGA GAATCTCATT 
CAGGAAAATG TGCAGTTGCT TGAGACAAAA AATGCATTGA ATGTGGTAAA GAATGATCTG 
ATCGCTCGCG TGGACGAGCT CTCCTGCGAG AAGGAGGTCC TTCAGGGGGA ACTGGATGCG 
GTTTTGCAGG CCAAAGCCAA ACTAGAGGAG AAATACAAGG AGCTGGAGGA GGAGCTCAAA 
AAAATAAAAA CCGAGACAGA GGAGGCCAAA CAGAACAATA ATAAAGAAGA AGATGAGAGT 
GATGTGCCCA CTGCTCAGAG ACGGAGGTTC ACGCGGGTGG AAATGGCTCG AGTGCTGATG 
GAGAGAAACC AGTACAAGGA GAGACTGATG GAGCTGCAGG AGGCCGTCCG GTGGACCGAA 
ATGATTCGAG CTTCAAGAGA AAATTCAGAA GTCGCAGAAA AGAAGAGGTC AAGCATCAGG 
CAATTCTTCA GTCGCCTCTT CAGTTCTTCC AGTTCTGCTG CGTCCAGCAG CTCGTCCAGA 
GCTTTACCGT CCAACGTCAA GTACAACCCA CCCAGCATCA TGAAGAAGAG CAACACCTTC 
TCCCAGTTCC CCAGAGAAAA GTCCAAAGCT TTCGACTTCC TCAAAGAAGA GGTGGACTCG 
AGTGGCTCTC TGTCCCGCCG GGAGCAGAAG CGGGCTCAGT ATCAGCATGT GAAAGCACAC 
ATGCAGAAAG AGGACGGCAG GGTGACCGCA CATGGCTGGA GTCTGCCGGG AAAATACAAG 
GTTGCCAATG GTGGCCCTGT AGAGAACAAA ATAAACCTGC CGGTTCCTGT GTGTCTCAAG 
CCACTGGAGC AGAAAGATGC TTCTATGAAG CTGCTGTGTG CCACTGGAGT GAATCTCTCT 
GGAGGACGCT TGTGTGACAG AGGCTCTGCT GAAGACTCCA CAGTAGAGAC AGACTCAGCC 
AAACAGAGGA AAGGCTCTCA GAGCAGCCTA GATCAGCTGG AGCACGATCC TAAGGAGCGG 
GAGCAGACGC TGCATGTGGA AATGTCCAGT CGCGTCTGGA TCTGCACGAG CACTCACTCC 
TCTACTAAGG TGATGGTCAT AGATGTGACG CGACCCGACG ACCTCCTGGA CAGCTTCTAC 
GTCTGTAACT CATGCGTGCT GTGTATCGCC AGCATACCAG GAGTGTTGGA GACAGATTAT 
CCTGCGGGGG AGGAGATTCC TCAGGATCTG GAGGCGGGAC CAGACGCTCT ATTGGCCAGC 
AGCAGCTCTC AGGGCAGTGA GGGAGTGTTG AGTGACGTGT CGGCGGTTGG CTGTGGTGTT 
GAGGAGTCCA GTTTAGGCTC CCAAGCGGGC AGCACAGGAG CCCACGAACA GTCTGCTGAA 
CAGAGTCCCG CCAGAGGAAG TGATCTCTCT AGAGCTCCCA GTGAAGATGG TCTCCCTGCC 
GCTGAGGAGG CTATGGAGGC CACTGAGGAG GACCAGGAGA GTCCGGGACA GGACAGCAGT 
CAGCCGGGTA TCTACACGGA GCATGTGTTT ACAGACCCGC TGGGACCCGA GTCTTCTGGG 
GCGTCAGCAG GCCATGATAG CCAGAGAGAG TCCGATCGGG ACAGGGATGA CACGAGCTCA 
GCAGGGGGCA AGACGGATCT GGCAAAAGAA GAAACGCAGC GGATGAGCAG TGCACTGCCC 
ACCATGTGGC TGGGGGCTCA GAACGGCTGT TTATATGTGC ACTCATCTGT TGCTCGATGG 
AGGAAGTGTC TTCATGCTGT CAAGCTAAAG GATTCCATCT TGAACATTGT ACATGTGAAA 
GGCCGTGTTT TGGTGGCTCT AGCTGACGGG ACACTGGCCA TCTTCCATAG AGGTCTTGAC 
AGCCAGTGGG ATTTAACCAA CTACCATCTT CTGGACCTGG GCCGGCCGCA TCACTCAATC 
CGCTGCATGA CTGTGGTTCA CGATAAAGTG TGGTGCGGCT ACAGGAACAA GATCTACATC 
ATTCAGCCCA AAGCTATGAA AATCGAGAGG TCTTTTGATG CCCATCCGCG TAAGGAGAGT 
CAGGTGCGTC AGCTGGCCTG GGTGGGTGAC GGGATCTGGG TGTCCATCCG GCTGGACTCA 
ACGCTTCGCC TATTCCATGC TCACACCTTC CAGCATCTGC AGGACGTAGA CATTGAGCCT 
TACGTCAGCA AAATGTTAGG AACTGGAAAA CTGGGCTTCT CTTTTGTGAG AATCACTGCT 
CTGATGGTTT CTTGTAATCG GCTGTGGGTG GGAACAGGAA ATGGTGTCAT CATCTCCATC 
CCTCTGTCAG ACTCTGTAGG TCAGTGTGGA TATCTAGGTC AGAGGGCAGC TAATAAGACG 
GTGGGAGCGA TTGCCTCTCG TCCTGGTGGA GCAGTGCGTG TATACAGCGA GGACGCTGTG 
GACGGTGGGG TGACCGGCTC ATACGTCCCG TACTGCTCCA TGGCACATGC TCAGCTCTGC 
TTCCATGGCC ACAGGGACGC TGTTAAATTT TTCGCTACAG TTCCAGGTCA GGTTATTGTG 
CCTGGAGCTG AGGGTGTGAC TGAGAGCTCT GAGTCTTCAG ATGGAAAGAG CATGTTGATC 
ATGAGTGGAG GAGAGGGATA CATCGACTTC AGAGTGGGTG ATGAGGATGG AGGATTGGGA 
CGAGATGAAG ATGAAGAATC AGCTGAACCC ACACTGAAAC TCCAGTCGGC TCCTGCCAAA 
CCTGAGCGCA GTTATGTTAT AGTCTGGCAG GTTGCGAGTG ACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307217.1
CDS	180..4127
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAGLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	NM_001320288.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320288.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGGTTT GACTGATAAA GAGGAGAAGT CAGATGTTCA GGCCATTATT 
GAATCCACAC CGGAGCTGGA CATGGACCTT GGATATAAAG GCCCAAGTAC GCCGACCCGA 
GGGATTGAGA ACATGGCGTT TGACCGCAAC ACAGAATCCC TGTTTGAAGA AGTGTCATCG 
GCGGGCACGG GACTGATCGG AGATGTGGAT GAAGGGGCCG ATCTGTTGGT GGAGTACTCT 
GTGCATGCGC TTTCACACCC GAGTCTGTCC GGCATGAGTC GTGAGGTGGA GAATCTCATT 
CAGGAAAATG TGCAGTTGCT TGAGACAAAA AATGCATTGA ATGTGGTAAA GAATGATCTG 
ATCGCTCGCG TGGACGAGCT CTCCTGCGAG AAGGAGGTCC TTCAGGGGGA ACTGGATGCG 
GTTTTGCAGG CCAAAGCCAA ACTAGAGGAG AAATACAAGG AGCTGGAGGA GGAGCTCAAA 
AAAATAAAAA CCGAGACAGA GGAGGCCAAA CAGAACAATA ATAAAGAAGA AGATGAGAGT 
GATGTGCCCA CTGCTCAGAG ACGGAGGTTC ACGCGGGTGG AAATGGCTCG AGTGCTGATG 
GAGAGAAACC AGTACAAGGA GAGACTGATG GAGCTGCAGG AGGCCGTCCG GTGGACCGAA 
ATGATTCGAG CTTCAAGAGA AAATTCAGAA GTCGCAGAAA AGAAGAGGTC AAGCATCAGG 
CAATTCTTCA GTCGCCTCTT CAGTTCTTCC AGTTCTGCTG CGTCCAGCAG CTCGTCCAGA 
GCTTTACCGT CCAACGTCAA GTACAACCCA CCCAGCATCA TGAAGAAGAG CAACACCTTC 
TCCCAGTTCC CCAGAGAAAA GTCCAAAGCT TTCGACTTCC TCAAAGAAGA GGTGGACTCG 
AGTGGCTCTC TGTCCCGCCG GGAGCAGAAG CGGGCTCAGT ATCAGCATGT GAAAGCACAC 
ATGCAGAAAG AGGACGGCAG GGTGACCGCA CATGGCTGGA GTCTGCCGGG AAAATACAAG 
GTTGCCAATG GTGGCCCTGT AGAGAACAAA ATAAACCTGC CGGTTCCTGT GTGTCTCAAG 
CCACTGGAGC AGAAAGATGC TTCTATGAAG CTGCTGTGTG CCACTGGAGT GAATCTCTCT 
GGAGGACGCT TGTGTGACAG AGGCTCTGCT GAAGACTCCA CAGTAGAGAC AGACTCAGCC 
AAACAGAGGA AAGGCTCTCA GAGCAGCCTA GATCAGCTGG AGCACGATCC TAAGGAGCGG 
GAGCAGACGC TGCATGTGGA AATGTCCAGT CGCGTCTGGA TCTGCACGAG CACTCACTCC 
TCTACTAAGG TGATGGTCAT AGATGTGACG CGACCCGACG ACCTCCTGGA CAGCTTCTAC 
GTCTGTAACT CATGCGTGCT GTGTATCGCC AGCATACCAG GAGTGTTGGA GACAGATTAT 
CCTGCGGGGG AGGAGATTCC TCAGGATCTG GAGGCGGGAC CAGACGCTCT ATTGGCCAGC 
AGCAGCTCTC AGGGCAGTGA GGGAGTGTTG AGTGACGTGT CGGCGGTTGG CTGTGGTGTT 
GAGGAGTCCA GTTTAGGCTC CCAAGCGGGC AGCACAGGAG CCCACGAACA GTCTGCTGAA 
CAGAGTCCCG CCAGAGGAAG TGATCTCTCT AGAGCTCCCA GTGAAGATGG TCTCCCTGCC 
GCTGAGGAGG CTATGGAGGC CACTGAGGAG GACCAGGAGA GTCCGGGACA GGACAGCAGT 
CAGCCGGGTA TCTACACGGA GCATGTGTTT ACAGACCCGC TGGGACCCGA GTCTTCTGGG 
GCGTCAGCAG GCCATGATAG CCAGAGAGAG TCCGATCGGG ACAGGGATGA CACGAGCTCA 
GCAGGGGGCA AGACGGATCT GGCAAAAGAA GAAACGCAGC GGATGAGCAG TGCACTGCCC 
ACCATGTGGC TGGGGGCTCA GAACGGCTGT TTATATGTGC ACTCATCTGT TGCTCGATGG 
AGGAAGTGTC TTCATGCTGT CAAGCTAAAG GATTCCATCT TGAACATTGT ACATGTGAAA 
GGCCGTGTTT TGGTGGCTCT AGCTGACGGG ACACTGGCCA TCTTCCATAG AGGTCTTGAC 
AGCCAGTGGG ATTTAACCAA CTACCATCTT CTGGACCTGG GCCGGCCGCA TCACTCAATC 
CGCTGCATGA CTGTGGTTCA CGATAAAGTG TGGTGCGGCT ACAGGAACAA GATCTACATC 
ATTCAGCCCA AAGCTATGAA AATCGAGAGG TCTTTTGATG CCCATCCGCG TAAGGAGAGT 
CAGGTGCGTC AGCTGGCCTG GGTGGGTGAC GGGATCTGGG TGTCCATCCG GCTGGACTCA 
ACGCTTCGCC TATTCCATGC TCACACCTTC CAGCATCTGC AGGACGTAGA CATTGAGCCT 
TACGTCAGCA AAATGTTAGG AACTGGAAAA CTGGGCTTCT CTTTTGTGAG AATCACTGCT 
CTGATGGTTT CTTGTAATCG GCTGTGGGTG GGAACAGGAA ATGGTGTCAT CATCTCCATC 
CCTCTGTCAG ACTCTGTAGG TCAGTGTGGA TATCTAGGTC AGAGGGCAGC TAATAAGACG 
GTGGGAGCGA TTGCCTCTCG TCCTGGTGGA GCAGTGCGTG TATACAGCGA GGACGCTGTG 
GACGGTGGGG TGACCGGCTC ATACGTCCCG TACTGCTCCA TGGCACATGC TCAGCTCTGC 
TTCCATGGCC ACAGGGACGC TGTTAAATTT TTCGCTACAG TTCCAGGTCA GGTTATTGTG 
CCTGGAGCTG AGGGTGTGAC TGAGAGCTCT GAGTCTTCAG ATGGAAAGAG CATGTTGATC 
ATGAGTGGAG GAGAGGGATA CATCGACTTC AGAGTGGGTG ATGAGGATGG AGGATTGGGA 
CGAGATGAAG ATGAAGAATC AGCTGAACCC ACACTGAAAC TCCAGTCGGC TCCTGCCAAA 
CCTGAGCGCA GTTATGTTAT AGTCTGGCAG GTTGCGAGTG ACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_021470820.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326495.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141583
Clone ID Related Accession (Same CDS sequence)	XM_021470820.1
Accession Version	XM_021470820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
GTAGGTCAGT GTGGATATCT AGGTCAGAGG GCTAATAAGA CGGTGGGAGC GATTGCCTCT 
CGTCCTGGTG GAGCAGTGCG TGTATACAGC GAGGACGCTG TGGACGGTGG GGTGACCGGC 
TCATACGTCC CGTACTGCTC CATGGCACAT GCTCAGCTCT GCTTCCATGG CCACAGGGAC 
GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT CAGGTTATTG TGCCTGGAGC TGAGGGTGTG 
ACTGAGAGCT CTGAGTCTTC AGATGGAAAG AGCATGTTGA TCATGAGTGG AGGAGAGGGA 
TACATCGACT TCAGAGTGGG TGATGAGGAT GGAGGATTGG GACGAGATGA AGATGAAGAA 
TCAGCTGAAC CCACACTGAA ACTCCAGTCG GCTCCTGCCA AACCTGAGCG CAGTTATGTT 
ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326495.1
CDS	579..4508
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_021470820.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470820.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
GTAGGTCAGT GTGGATATCT AGGTCAGAGG GCTAATAAGA CGGTGGGAGC GATTGCCTCT 
CGTCCTGGTG GAGCAGTGCG TGTATACAGC GAGGACGCTG TGGACGGTGG GGTGACCGGC 
TCATACGTCC CGTACTGCTC CATGGCACAT GCTCAGCTCT GCTTCCATGG CCACAGGGAC 
GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT CAGGTTATTG TGCCTGGAGC TGAGGGTGTG 
ACTGAGAGCT CTGAGTCTTC AGATGGAAAG AGCATGTTGA TCATGAGTGG AGGAGAGGGA 
TACATCGACT TCAGAGTGGG TGATGAGGAT GGAGGATTGG GACGAGATGA AGATGAAGAA 
TCAGCTGAAC CCACACTGAA ACTCCAGTCG GCTCCTGCCA AACCTGAGCG CAGTTATGTT 
ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_021470831.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326506.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X4, mRNA.

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CloneID	ODa141584
Clone ID Related Accession (Same CDS sequence)	XM_021470831.1
Accession Version	XM_021470831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGGTTT GACTGATAAA GAGGAGAAGT CAGATGTTCA GGCCATTATT 
GAATCCACAC CGGAGCTGGA CATGGACCTT GGATATAAAG GCCCAAGTAC GCCGACCCGA 
GGGATTGAGA ACATGGCGTT TGACCGCAAC ACAGAATCCC TGTTTGAAGA AGTGTCATCG 
GCGGGCACGG GACTGATCGG AGATGTGGAT GAAGGGGCCG ATCTGTTGGT GGAGTACTCT 
GGCATGAGTC GTGAGGTGGA GAATCTCATT CAGGAAAATG TGCAGTTGCT TGAGACAAAA 
AATGCATTGA ATGTGGTAAA GAATGATCTG ATCGCTCGCG TGGACGAGCT CTCCTGCGAG 
AAGGAGGTCC TTCAGGGGGA ACTGGATGCG GTTTTGCAGG CCAAAGCCAA ACTAGAGGAG 
AAATACAAGG AGCTGGAGGA GGAGCTCAAA AAAATAAAAA CCGAGACAGA GGAGGCCAAA 
CAGAACAATA ATAAAGAAGA AGATGAGAGT GATGTGCCCA CTGCTCAGAG ACGGAGGTTC 
ACGCGGGTGG AAATGGCTCG AGTGCTGATG GAGAGAAACC AGTACAAGGA GAGACTGATG 
GAGCTGCAGG AGGCCGTCCG GTGGACCGAA ATGATTCGAG CTTCAAGAGA AAATTCAGAA 
GTCGCAGAAA AGAAGAGGTC AAGCATCAGG CAATTCTTCA GTCGCCTCTT CAGTTCTTCC 
AGTTCTGCTG CGTCCAGCAG CTCGTCCAGA GCTTTACCGT CCAACGTCAA GTACAACCCA 
CCCAGCATCA TGAAGAAGAG CAACACCTTC TCCCAGTTCC CCAGAGAAAA GTCCAAAGCT 
TTCGACTTCC TCAAAGAAGA GGTGGACTCG AGTGGCTCTC TGTCCCGCCG GGAGCAGAAG 
CGGGCTCAGT ATCAGCATGT GAAAGCACAC ATGCAGAAAG AGGACGGCAG GGTGACCGCA 
CATGGCTGGA GTCTGCCGGG AAAATACAAG GTTGCCAATG GTGGCCCTGT AGAGAACAAA 
ATAAACCTGC CGGTTCCTGT GTGTCTCAAG CCACTGGAGC AGAAAGATGC TTCTATGAAG 
CTGCTGTGTG CCACTGGAGT GAATCTCTCT GGAGGACGCT TGTGTGACAG AGGCTCTGCT 
GAAGACTCCA CAGTAGAGAC AGACTCAGCC AAACAGAGGA AAGGCTCTCA GAGCAGCCTA 
GATCAGCTGG AGCACGATCC TAAGGAGCGG GAGCAGACGC TGCATGTGGA AATGTCCAGT 
CGCGTCTGGA TCTGCACGAG CACTCACTCC TCTACTAAGG TGATGGTCAT AGATGTGACG 
CGACCCGACG ACCTCCTGGA CAGCTTCTAC GTCTGTAACT CATGCGTGCT GTGTATCGCC 
AGCATACCAG GAGTGTTGGA GACAGATTAT CCTGCGGGGG AGGAGATTCC TCAGGATCTG 
GAGGCGGGAC CAGACGCTCT ATTGGCCAGC AGCAGCTCTC AGGGCAGTGA GGGAGTGTTG 
AGTGACGTGT CGGCGGTTGG CTGTGGTGTT GAGGAGTCCA GTTTAGGCTC CCAAGCGGGC 
AGCACAGGAG CCCACGAACA GTCTGCTGAA CAGAGTCCCG CCAGAGGAAG TGATCTCTCT 
AGAGCTCCCA GTGAAGATGG TCTCCCTGCC GCTGAGGAGG CTATGGAGGC CACTGAGGAG 
GACCAGGAGA GTCCGGGACA GGACAGCAGT CAGCCGGGTA TCTACACGGA GCATGTGTTT 
ACAGACCCGC TGGGACCCGA GTCTTCTGGG GCGTCAGCAG GCCATGATAG CCAGAGAGAG 
TCCGATCGGG ACAGGGATGA CACGAGCTCA GCAGGGGGCA AGACGGATCT GGCAAAAGAA 
GAAACGCAGC GGATGAGCAG TGCACTGCCC ACCATGTGGC TGGGGGCTCA GAACGGCTGT 
TTATATGTGC ACTCATCTGT TGCTCGATGG AGGAAGTGTC TTCATGCTGT CAAGCTAAAG 
GATTCCATCT TGAACATTGT ACATGTGAAA GGCCGTGTTT TGGTGGCTCT AGCTGACGGG 
ACACTGGCCA TCTTCCATAG AGGTCTTGAC AGCCAGTGGG ATTTAACCAA CTACCATCTT 
CTGGACCTGG GCCGGCCGCA TCACTCAATC CGCTGCATGA CTGTGGTTCA CGATAAAGTG 
TGGTGCGGCT ACAGGAACAA GATCTACATC ATTCAGCCCA AAGCTATGAA AATCGAGAGG 
TCTTTTGATG CCCATCCGCG TAAGGAGAGT CAGGTGCGTC AGCTGGCCTG GGTGGGTGAC 
GGGATCTGGG TGTCCATCCG GCTGGACTCA ACGCTTCGCC TATTCCATGC TCACACCTTC 
CAGCATCTGC AGGACGTAGA CATTGAGCCT TACGTCAGCA AAATGTTAGG AACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATCACTGCT CTGATGGTTT CTTGTAATCG GCTGTGGGTG 
GGAACAGGAA ATGGTGTCAT CATCTCCATC CCTCTGTCAG ACTCTGTAGG TCAGTGTGGA 
TATCTAGGTC AGAGGGCAGC TAATAAGACG GTGGGAGCGA TTGCCTCTCG TCCTGGTGGA 
GCAGTGCGTG TATACAGCGA GGACGCTGTG GACGGTGGGG TGACCGGCTC ATACGTCCCG 
TACTGCTCCA TGGCACATGC TCAGCTCTGC TTCCATGGCC ACAGGGACGC TGTTAAATTT 
TTCGCTACAG TTCCAGGTCA GGTTATTGTG CCTGGAGCTG AGGGTGTGAC TGAGAGCTCT 
GAGTCTTCAG ATGGAAAGAG CATGTTGATC ATGAGTGGAG GAGAGGGATA CATCGACTTC 
AGAGTGGGTG ATGAGGATGG AGGATTGGGA CGAGATGAAG ATGAAGAATC AGCTGAACCC 
ACACTGAAAC TCCAGTCGGC TCCTGCCAAA CCTGAGCGCA GTTATGTTAT AGTCTGGCAG 
GTTGCGAGTG ACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326506.1
CDS	579..4496
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAGLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_021470831.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470831.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGGTTT GACTGATAAA GAGGAGAAGT CAGATGTTCA GGCCATTATT 
GAATCCACAC CGGAGCTGGA CATGGACCTT GGATATAAAG GCCCAAGTAC GCCGACCCGA 
GGGATTGAGA ACATGGCGTT TGACCGCAAC ACAGAATCCC TGTTTGAAGA AGTGTCATCG 
GCGGGCACGG GACTGATCGG AGATGTGGAT GAAGGGGCCG ATCTGTTGGT GGAGTACTCT 
GGCATGAGTC GTGAGGTGGA GAATCTCATT CAGGAAAATG TGCAGTTGCT TGAGACAAAA 
AATGCATTGA ATGTGGTAAA GAATGATCTG ATCGCTCGCG TGGACGAGCT CTCCTGCGAG 
AAGGAGGTCC TTCAGGGGGA ACTGGATGCG GTTTTGCAGG CCAAAGCCAA ACTAGAGGAG 
AAATACAAGG AGCTGGAGGA GGAGCTCAAA AAAATAAAAA CCGAGACAGA GGAGGCCAAA 
CAGAACAATA ATAAAGAAGA AGATGAGAGT GATGTGCCCA CTGCTCAGAG ACGGAGGTTC 
ACGCGGGTGG AAATGGCTCG AGTGCTGATG GAGAGAAACC AGTACAAGGA GAGACTGATG 
GAGCTGCAGG AGGCCGTCCG GTGGACCGAA ATGATTCGAG CTTCAAGAGA AAATTCAGAA 
GTCGCAGAAA AGAAGAGGTC AAGCATCAGG CAATTCTTCA GTCGCCTCTT CAGTTCTTCC 
AGTTCTGCTG CGTCCAGCAG CTCGTCCAGA GCTTTACCGT CCAACGTCAA GTACAACCCA 
CCCAGCATCA TGAAGAAGAG CAACACCTTC TCCCAGTTCC CCAGAGAAAA GTCCAAAGCT 
TTCGACTTCC TCAAAGAAGA GGTGGACTCG AGTGGCTCTC TGTCCCGCCG GGAGCAGAAG 
CGGGCTCAGT ATCAGCATGT GAAAGCACAC ATGCAGAAAG AGGACGGCAG GGTGACCGCA 
CATGGCTGGA GTCTGCCGGG AAAATACAAG GTTGCCAATG GTGGCCCTGT AGAGAACAAA 
ATAAACCTGC CGGTTCCTGT GTGTCTCAAG CCACTGGAGC AGAAAGATGC TTCTATGAAG 
CTGCTGTGTG CCACTGGAGT GAATCTCTCT GGAGGACGCT TGTGTGACAG AGGCTCTGCT 
GAAGACTCCA CAGTAGAGAC AGACTCAGCC AAACAGAGGA AAGGCTCTCA GAGCAGCCTA 
GATCAGCTGG AGCACGATCC TAAGGAGCGG GAGCAGACGC TGCATGTGGA AATGTCCAGT 
CGCGTCTGGA TCTGCACGAG CACTCACTCC TCTACTAAGG TGATGGTCAT AGATGTGACG 
CGACCCGACG ACCTCCTGGA CAGCTTCTAC GTCTGTAACT CATGCGTGCT GTGTATCGCC 
AGCATACCAG GAGTGTTGGA GACAGATTAT CCTGCGGGGG AGGAGATTCC TCAGGATCTG 
GAGGCGGGAC CAGACGCTCT ATTGGCCAGC AGCAGCTCTC AGGGCAGTGA GGGAGTGTTG 
AGTGACGTGT CGGCGGTTGG CTGTGGTGTT GAGGAGTCCA GTTTAGGCTC CCAAGCGGGC 
AGCACAGGAG CCCACGAACA GTCTGCTGAA CAGAGTCCCG CCAGAGGAAG TGATCTCTCT 
AGAGCTCCCA GTGAAGATGG TCTCCCTGCC GCTGAGGAGG CTATGGAGGC CACTGAGGAG 
GACCAGGAGA GTCCGGGACA GGACAGCAGT CAGCCGGGTA TCTACACGGA GCATGTGTTT 
ACAGACCCGC TGGGACCCGA GTCTTCTGGG GCGTCAGCAG GCCATGATAG CCAGAGAGAG 
TCCGATCGGG ACAGGGATGA CACGAGCTCA GCAGGGGGCA AGACGGATCT GGCAAAAGAA 
GAAACGCAGC GGATGAGCAG TGCACTGCCC ACCATGTGGC TGGGGGCTCA GAACGGCTGT 
TTATATGTGC ACTCATCTGT TGCTCGATGG AGGAAGTGTC TTCATGCTGT CAAGCTAAAG 
GATTCCATCT TGAACATTGT ACATGTGAAA GGCCGTGTTT TGGTGGCTCT AGCTGACGGG 
ACACTGGCCA TCTTCCATAG AGGTCTTGAC AGCCAGTGGG ATTTAACCAA CTACCATCTT 
CTGGACCTGG GCCGGCCGCA TCACTCAATC CGCTGCATGA CTGTGGTTCA CGATAAAGTG 
TGGTGCGGCT ACAGGAACAA GATCTACATC ATTCAGCCCA AAGCTATGAA AATCGAGAGG 
TCTTTTGATG CCCATCCGCG TAAGGAGAGT CAGGTGCGTC AGCTGGCCTG GGTGGGTGAC 
GGGATCTGGG TGTCCATCCG GCTGGACTCA ACGCTTCGCC TATTCCATGC TCACACCTTC 
CAGCATCTGC AGGACGTAGA CATTGAGCCT TACGTCAGCA AAATGTTAGG AACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATCACTGCT CTGATGGTTT CTTGTAATCG GCTGTGGGTG 
GGAACAGGAA ATGGTGTCAT CATCTCCATC CCTCTGTCAG ACTCTGTAGG TCAGTGTGGA 
TATCTAGGTC AGAGGGCAGC TAATAAGACG GTGGGAGCGA TTGCCTCTCG TCCTGGTGGA 
GCAGTGCGTG TATACAGCGA GGACGCTGTG GACGGTGGGG TGACCGGCTC ATACGTCCCG 
TACTGCTCCA TGGCACATGC TCAGCTCTGC TTCCATGGCC ACAGGGACGC TGTTAAATTT 
TTCGCTACAG TTCCAGGTCA GGTTATTGTG CCTGGAGCTG AGGGTGTGAC TGAGAGCTCT 
GAGTCTTCAG ATGGAAAGAG CATGTTGATC ATGAGTGGAG GAGAGGGATA CATCGACTTC 
AGAGTGGGTG ATGAGGATGG AGGATTGGGA CGAGATGAAG ATGAAGAATC AGCTGAACCC 
ACACTGAAAC TCCAGTCGGC TCCTGCCAAA CCTGAGCGCA GTTATGTTAT AGTCTGGCAG 
GTTGCGAGTG ACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_021470832.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326507.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X5, mRNA.

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CloneID	ODa141585
Clone ID Related Accession (Same CDS sequence)	XM_021470832.1
Accession Version	XM_021470832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCA 
GCTAATAAGA CGGTGGGAGC GATTGCCTCT CGTCCTGGTG GAGCAGTGCG TGTATACAGC 
GAGGACGCTG TGGACGGTGG GGTGACCGGC TCATACGTCC CGTACTGCTC CATGGCACAT 
GCTCAGCTCT GCTTCCATGG CCACAGGGAC GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT 
CAGGTTATTG TGCCTGGAGC TGAGGGTGTG ACTGAGAGCT CTGAGTCTTC AGATGGAAAG 
AGCATGTTGA TCATGAGTGG AGGAGAGGGA TACATCGACT TCAGAGTGGG TGATGAGGAT 
GGAGGATTGG GACGAGATGA AGATGAAGAA TCAGCTGAAC CCACACTGAA ACTCCAGTCG 
GCTCCTGCCA AACCTGAGCG CAGTTATGTT ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326507.1
CDS	579..4478
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_021470832.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470832.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCA 
GCTAATAAGA CGGTGGGAGC GATTGCCTCT CGTCCTGGTG GAGCAGTGCG TGTATACAGC 
GAGGACGCTG TGGACGGTGG GGTGACCGGC TCATACGTCC CGTACTGCTC CATGGCACAT 
GCTCAGCTCT GCTTCCATGG CCACAGGGAC GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT 
CAGGTTATTG TGCCTGGAGC TGAGGGTGTG ACTGAGAGCT CTGAGTCTTC AGATGGAAAG 
AGCATGTTGA TCATGAGTGG AGGAGAGGGA TACATCGACT TCAGAGTGGG TGATGAGGAT 
GGAGGATTGG GACGAGATGA AGATGAAGAA TCAGCTGAAC CCACACTGAA ACTCCAGTCG 
GCTCCTGCCA AACCTGAGCG CAGTTATGTT ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_021470833.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326508.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa141586
Clone ID Related Accession (Same CDS sequence)	XM_021470833.1
Accession Version	XM_021470833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
AATAAGACGG TGGGAGCGAT TGCCTCTCGT CCTGGTGGAG CAGTGCGTGT ATACAGCGAG 
GACGCTGTGG ACGGTGGGGT GACCGGCTCA TACGTCCCGT ACTGCTCCAT GGCACATGCT 
CAGCTCTGCT TCCATGGCCA CAGGGACGCT GTTAAATTTT TCGCTACAGT TCCAGGTCAG 
GTTATTGTGC CTGGAGCTGA GGGTGTGACT GAGAGCTCTG AGTCTTCAGA TGGAAAGAGC 
ATGTTGATCA TGAGTGGAGG AGAGGGATAC ATCGACTTCA GAGTGGGTGA TGAGGATGGA 
GGATTGGGAC GAGATGAAGA TGAAGAATCA GCTGAACCCA CACTGAAACT CCAGTCGGCT 
CCTGCCAAAC CTGAGCGCAG TTATGTTATA GTCTGGCAGG TTGCGAGTGA CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326508.1
CDS	579..4475
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSNKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_021470833.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470833.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
AATAAGACGG TGGGAGCGAT TGCCTCTCGT CCTGGTGGAG CAGTGCGTGT ATACAGCGAG 
GACGCTGTGG ACGGTGGGGT GACCGGCTCA TACGTCCCGT ACTGCTCCAT GGCACATGCT 
CAGCTCTGCT TCCATGGCCA CAGGGACGCT GTTAAATTTT TCGCTACAGT TCCAGGTCAG 
GTTATTGTGC CTGGAGCTGA GGGTGTGACT GAGAGCTCTG AGTCTTCAGA TGGAAAGAGC 
ATGTTGATCA TGAGTGGAGG AGAGGGATAC ATCGACTTCA GAGTGGGTGA TGAGGATGGA 
GGATTGGGAC GAGATGAAGA TGAAGAATCA GCTGAACCCA CACTGAAACT CCAGTCGGCT 
CCTGCCAAAC CTGAGCGCAG TTATGTTATA GTCTGGCAGG TTGCGAGTGA CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_021470835.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326510.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa141587
Clone ID Related Accession (Same CDS sequence)	XM_021470835.1
Accession Version	XM_021470835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGGATGA GCTGTCTGAC CTCAGCCAGG GCGGCTCCAA GTCTGTGACC 
CCCATGTCCA CCACAGCCTC TGACCTGGCC CTGGATTCAC ACAGAGAAGA CGGAGACGCT 
TTCTGGAAGA ACACAGAGGT GCAGGCTGTT CCCGGGACCA GAAGCGTCTC TGCAGAAGTG 
CGAGTAAATA GTTTGACTGA TAAAGAGGAG AAGTCAGATG TTCAGGCCAT TATTGAATCC 
ACACCGGAGC TGGACATGGA CCTTGGATAT AAAGGCCCAA GTACGCCGAC CCGAGGGATT 
GAGAACATGG CGTTTGACCG CAACACAGAA TCCCTGTTTG AAGAAGTGTC ATCGGCGGGC 
ACGGGACTGA TCGGAGATGT GGATGAAGGG GCCGATCTGT TGGTGGAGTA CTCTGGCATG 
AGTCGTGAGG TGGAGAATCT CATTCAGGAA AATGTGCAGT TGCTTGAGAC AAAAAATGCA 
TTGAATGTGG TAAAGAATGA TCTGATCGCT CGCGTGGACG AGCTCTCCTG CGAGAAGGAG 
GTCCTTCAGG GGGAACTGGA TGCGGTTTTG CAGGCCAAAG CCAAACTAGA GGAGAAATAC 
AAGGAGCTGG AGGAGGAGCT CAAAAAAATA AAAACCGAGA CAGAGGAGGC CAAACAGAAC 
AATAATAAAG AAGAAGATGA GAGTGATGTG CCCACTGCTC AGAGACGGAG GTTCACGCGG 
GTGGAAATGG CTCGAGTGCT GATGGAGAGA AACCAGTACA AGGAGAGACT GATGGAGCTG 
CAGGAGGCCG TCCGGTGGAC CGAAATGATT CGAGCTTCAA GAGAAAATTC AGAAGTCGCA 
GAAAAGAAGA GGTCAAGCAT CAGGCAATTC TTCAGTCGCC TCTTCAGTTC TTCCAGTTCT 
GCTGCGTCCA GCAGCTCGTC CAGAGCTTTA CCGTCCAACG TCAAGTACAA CCCACCCAGC 
ATCATGAAGA AGAGCAACAC CTTCTCCCAG TTCCCCAGAG AAAAGTCCAA AGCTTTCGAC 
TTCCTCAAAG AAGAGGTGGA CTCGAGTGGC TCTCTGTCCC GCCGGGAGCA GAAGCGGGCT 
CAGTATCAGC ATGTGAAAGC ACACATGCAG AAAGAGGACG GCAGGGTGAC CGCACATGGC 
TGGAGTCTGC CGGGAAAATA CAAGGTTGCC AATGGTGGCC CTGTAGAGAA CAAAATAAAC 
CTGCCGGTTC CTGTGTGTCT CAAGCCACTG GAGCAGAAAG ATGCTTCTAT GAAGCTGCTG 
TGTGCCACTG GAGTGAATCT CTCTGGAGGA CGCTTGTGTG ACAGAGGCTC TGCTGAAGAC 
TCCACAGTAG AGACAGACTC AGCCAAACAG AGGAAAGGCT CTCAGAGCAG CCTAGATCAG 
CTGGAGCACG ATCCTAAGGA GCGGGAGCAG ACGCTGCATG TGGAAATGTC CAGTCGCGTC 
TGGATCTGCA CGAGCACTCA CTCCTCTACT AAGGTGATGG TCATAGATGT GACGCGACCC 
GACGACCTCC TGGACAGCTT CTACGTCTGT AACTCATGCG TGCTGTGTAT CGCCAGCATA 
CCAGGAGTGT TGGAGACAGA TTATCCTGCG GGGGAGGAGA TTCCTCAGGA TCTGGAGGCG 
GGACCAGACG CTCTATTGGC CAGCAGCAGC TCTCAGGGCA GTGAGGGAGT GTTGAGTGAC 
GTGTCGGCGG TTGGCTGTGG TGTTGAGGAG TCCAGTTTAG GCTCCCAAGC GGGCAGCACA 
GGAGCCCACG AACAGTCTGC TGAACAGAGT CCCGCCAGAG GAAGTGATCT CTCTAGAGCT 
CCCAGTGAAG ATGGTCTCCC TGCCGCTGAG GAGGCTATGG AGGCCACTGA GGAGGACCAG 
GAGAGTCCGG GACAGGACAG CAGTCAGCCG GGTATCTACA CGGAGCATGT GTTTACAGAC 
CCGCTGGGAC CCGAGTCTTC TGGGGCGTCA GCAGGCCATG ATAGCCAGAG AGAGTCCGAT 
CGGGACAGGG ATGACACGAG CTCAGCAGGG GGCAAGACGG ATCTGGCAAA AGAAGAAACG 
CAGCGGATGA GCAGTGCACT GCCCACCATG TGGCTGGGGG CTCAGAACGG CTGTTTATAT 
GTGCACTCAT CTGTTGCTCG ATGGAGGAAG TGTCTTCATG CTGTCAAGCT AAAGGATTCC 
ATCTTGAACA TTGTACATGT GAAAGGCCGT GTTTTGGTGG CTCTAGCTGA CGGGACACTG 
GCCATCTTCC ATAGAGGTCT TGACAGCCAG TGGGATTTAA CCAACTACCA TCTTCTGGAC 
CTGGGCCGGC CGCATCACTC AATCCGCTGC ATGACTGTGG TTCACGATAA AGTGTGGTGC 
GGCTACAGGA ACAAGATCTA CATCATTCAG CCCAAAGCTA TGAAAATCGA GAGGTCTTTT 
GATGCCCATC CGCGTAAGGA GAGTCAGGTG CGTCAGCTGG CCTGGGTGGG TGACGGGATC 
TGGGTGTCCA TCCGGCTGGA CTCAACGCTT CGCCTATTCC ATGCTCACAC CTTCCAGCAT 
CTGCAGGACG TAGACATTGA GCCTTACGTC AGCAAAATGT TAGGAACTGG AAAACTGGGC 
TTCTCTTTTG TGAGAATCAC TGCTCTGATG GTTTCTTGTA ATCGGCTGTG GGTGGGAACA 
GGAAATGGTG TCATCATCTC CATCCCTCTG TCAGACTCTG TAGGTCAGTG TGGATATCTA 
GGTCAGAGGG CAGCTAATAA GACGGTGGGA GCGATTGCCT CTCGTCCTGG TGGAGCAGTG 
CGTGTATACA GCGAGGACGC TGTGGACGGT GGGGTGACCG GCTCATACGT CCCGTACTGC 
TCCATGGCAC ATGCTCAGCT CTGCTTCCAT GGCCACAGGG ACGCTGTTAA ATTTTTCGCT 
ACAGTTCCAG GTCAGGTTAT TGTGCCTGGA GCTGAGGGTG TGACTGAGAG CTCTGAGTCT 
TCAGATGGAA AGAGCATGTT GATCATGAGT GGAGGAGAGG GATACATCGA CTTCAGAGTG 
GGTGATGAGG ATGGAGGATT GGGACGAGAT GAAGATGAAG AATCAGCTGA ACCCACACTG 
AAACTCCAGT CGGCTCCTGC CAAACCTGAG CGCAGTTATG TTATAGTCTG GCAGGTTGCG 
AGTGACCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326510.1
CDS	579..4430
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_021470835.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470835.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGGATGA GCTGTCTGAC CTCAGCCAGG GCGGCTCCAA GTCTGTGACC 
CCCATGTCCA CCACAGCCTC TGACCTGGCC CTGGATTCAC ACAGAGAAGA CGGAGACGCT 
TTCTGGAAGA ACACAGAGGT GCAGGCTGTT CCCGGGACCA GAAGCGTCTC TGCAGAAGTG 
CGAGTAAATA GTTTGACTGA TAAAGAGGAG AAGTCAGATG TTCAGGCCAT TATTGAATCC 
ACACCGGAGC TGGACATGGA CCTTGGATAT AAAGGCCCAA GTACGCCGAC CCGAGGGATT 
GAGAACATGG CGTTTGACCG CAACACAGAA TCCCTGTTTG AAGAAGTGTC ATCGGCGGGC 
ACGGGACTGA TCGGAGATGT GGATGAAGGG GCCGATCTGT TGGTGGAGTA CTCTGGCATG 
AGTCGTGAGG TGGAGAATCT CATTCAGGAA AATGTGCAGT TGCTTGAGAC AAAAAATGCA 
TTGAATGTGG TAAAGAATGA TCTGATCGCT CGCGTGGACG AGCTCTCCTG CGAGAAGGAG 
GTCCTTCAGG GGGAACTGGA TGCGGTTTTG CAGGCCAAAG CCAAACTAGA GGAGAAATAC 
AAGGAGCTGG AGGAGGAGCT CAAAAAAATA AAAACCGAGA CAGAGGAGGC CAAACAGAAC 
AATAATAAAG AAGAAGATGA GAGTGATGTG CCCACTGCTC AGAGACGGAG GTTCACGCGG 
GTGGAAATGG CTCGAGTGCT GATGGAGAGA AACCAGTACA AGGAGAGACT GATGGAGCTG 
CAGGAGGCCG TCCGGTGGAC CGAAATGATT CGAGCTTCAA GAGAAAATTC AGAAGTCGCA 
GAAAAGAAGA GGTCAAGCAT CAGGCAATTC TTCAGTCGCC TCTTCAGTTC TTCCAGTTCT 
GCTGCGTCCA GCAGCTCGTC CAGAGCTTTA CCGTCCAACG TCAAGTACAA CCCACCCAGC 
ATCATGAAGA AGAGCAACAC CTTCTCCCAG TTCCCCAGAG AAAAGTCCAA AGCTTTCGAC 
TTCCTCAAAG AAGAGGTGGA CTCGAGTGGC TCTCTGTCCC GCCGGGAGCA GAAGCGGGCT 
CAGTATCAGC ATGTGAAAGC ACACATGCAG AAAGAGGACG GCAGGGTGAC CGCACATGGC 
TGGAGTCTGC CGGGAAAATA CAAGGTTGCC AATGGTGGCC CTGTAGAGAA CAAAATAAAC 
CTGCCGGTTC CTGTGTGTCT CAAGCCACTG GAGCAGAAAG ATGCTTCTAT GAAGCTGCTG 
TGTGCCACTG GAGTGAATCT CTCTGGAGGA CGCTTGTGTG ACAGAGGCTC TGCTGAAGAC 
TCCACAGTAG AGACAGACTC AGCCAAACAG AGGAAAGGCT CTCAGAGCAG CCTAGATCAG 
CTGGAGCACG ATCCTAAGGA GCGGGAGCAG ACGCTGCATG TGGAAATGTC CAGTCGCGTC 
TGGATCTGCA CGAGCACTCA CTCCTCTACT AAGGTGATGG TCATAGATGT GACGCGACCC 
GACGACCTCC TGGACAGCTT CTACGTCTGT AACTCATGCG TGCTGTGTAT CGCCAGCATA 
CCAGGAGTGT TGGAGACAGA TTATCCTGCG GGGGAGGAGA TTCCTCAGGA TCTGGAGGCG 
GGACCAGACG CTCTATTGGC CAGCAGCAGC TCTCAGGGCA GTGAGGGAGT GTTGAGTGAC 
GTGTCGGCGG TTGGCTGTGG TGTTGAGGAG TCCAGTTTAG GCTCCCAAGC GGGCAGCACA 
GGAGCCCACG AACAGTCTGC TGAACAGAGT CCCGCCAGAG GAAGTGATCT CTCTAGAGCT 
CCCAGTGAAG ATGGTCTCCC TGCCGCTGAG GAGGCTATGG AGGCCACTGA GGAGGACCAG 
GAGAGTCCGG GACAGGACAG CAGTCAGCCG GGTATCTACA CGGAGCATGT GTTTACAGAC 
CCGCTGGGAC CCGAGTCTTC TGGGGCGTCA GCAGGCCATG ATAGCCAGAG AGAGTCCGAT 
CGGGACAGGG ATGACACGAG CTCAGCAGGG GGCAAGACGG ATCTGGCAAA AGAAGAAACG 
CAGCGGATGA GCAGTGCACT GCCCACCATG TGGCTGGGGG CTCAGAACGG CTGTTTATAT 
GTGCACTCAT CTGTTGCTCG ATGGAGGAAG TGTCTTCATG CTGTCAAGCT AAAGGATTCC 
ATCTTGAACA TTGTACATGT GAAAGGCCGT GTTTTGGTGG CTCTAGCTGA CGGGACACTG 
GCCATCTTCC ATAGAGGTCT TGACAGCCAG TGGGATTTAA CCAACTACCA TCTTCTGGAC 
CTGGGCCGGC CGCATCACTC AATCCGCTGC ATGACTGTGG TTCACGATAA AGTGTGGTGC 
GGCTACAGGA ACAAGATCTA CATCATTCAG CCCAAAGCTA TGAAAATCGA GAGGTCTTTT 
GATGCCCATC CGCGTAAGGA GAGTCAGGTG CGTCAGCTGG CCTGGGTGGG TGACGGGATC 
TGGGTGTCCA TCCGGCTGGA CTCAACGCTT CGCCTATTCC ATGCTCACAC CTTCCAGCAT 
CTGCAGGACG TAGACATTGA GCCTTACGTC AGCAAAATGT TAGGAACTGG AAAACTGGGC 
TTCTCTTTTG TGAGAATCAC TGCTCTGATG GTTTCTTGTA ATCGGCTGTG GGTGGGAACA 
GGAAATGGTG TCATCATCTC CATCCCTCTG TCAGACTCTG TAGGTCAGTG TGGATATCTA 
GGTCAGAGGG CAGCTAATAA GACGGTGGGA GCGATTGCCT CTCGTCCTGG TGGAGCAGTG 
CGTGTATACA GCGAGGACGC TGTGGACGGT GGGGTGACCG GCTCATACGT CCCGTACTGC 
TCCATGGCAC ATGCTCAGCT CTGCTTCCAT GGCCACAGGG ACGCTGTTAA ATTTTTCGCT 
ACAGTTCCAG GTCAGGTTAT TGTGCCTGGA GCTGAGGGTG TGACTGAGAG CTCTGAGTCT 
TCAGATGGAA AGAGCATGTT GATCATGAGT GGAGGAGAGG GATACATCGA CTTCAGAGTG 
GGTGATGAGG ATGGAGGATT GGGACGAGAT GAAGATGAAG AATCAGCTGA ACCCACACTG 
AAACTCCAGT CGGCTCCTGC CAAACCTGAG CGCAGTTATG TTATAGTCTG GCAGGTTGCG 
AGTGACCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354364.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209853.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X1, mRNA.

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CloneID	ODa58055
Clone ID Related Accession (Same CDS sequence)	XM_017354364.1
Accession Version	XM_017354364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCT GTAGGTCAGT GTGGATATCT AGGTCAGAGG 
GCAGCTAATA AGACGGTGGG AGCGATTGCC TCTCGTCCTG GTGGAGCAGT GCGTGTATAC 
AGCGAGGACG CTGTGGACGG TGGGGTGACC GGCTCATACG TCCCGTACTG CTCCATGGCA 
CATGCTCAGC TCTGCTTCCA TGGCCACAGG GACGCTGTTA AATTTTTCGC TACAGTTCCA 
GGTCAGGTTA TTGTGCCTGG AGCTGAGGGT GTGACTGAGA GCTCTGAGTC TTCAGATGGA 
AAGAGCATGT TGATCATGAG TGGAGGAGAG GGATACATCG ACTTCAGAGT GGGTGATGAG 
GATGGAGGAT TGGGACGAGA TGAAGATGAA GAATCAGCTG AACCCACACT GAAACTCCAG 
TCGGCTCCTG CCAAACCTGA GCGCAGTTAT GTTATAGTCT GGCAGGTTGC GAGTGACCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209853.1
CDS	579..4541
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354364.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354364.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCT GTAGGTCAGT GTGGATATCT AGGTCAGAGG 
GCAGCTAATA AGACGGTGGG AGCGATTGCC TCTCGTCCTG GTGGAGCAGT GCGTGTATAC 
AGCGAGGACG CTGTGGACGG TGGGGTGACC GGCTCATACG TCCCGTACTG CTCCATGGCA 
CATGCTCAGC TCTGCTTCCA TGGCCACAGG GACGCTGTTA AATTTTTCGC TACAGTTCCA 
GGTCAGGTTA TTGTGCCTGG AGCTGAGGGT GTGACTGAGA GCTCTGAGTC TTCAGATGGA 
AAGAGCATGT TGATCATGAG TGGAGGAGAG GGATACATCG ACTTCAGAGT GGGTGATGAG 
GATGGAGGAT TGGGACGAGA TGAAGATGAA GAATCAGCTG AACCCACACT GAAACTCCAG 
TCGGCTCCTG CCAAACCTGA GCGCAGTTAT GTTATAGTCT GGCAGGTTGC GAGTGACCGA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354367.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209856.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa58056
Clone ID Related Accession (Same CDS sequence)	XM_017354367.1
Accession Version	XM_017354367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCT GTAGGTCAGT GTGGATATCT AGGTCAGAGG 
GCTAATAAGA CGGTGGGAGC GATTGCCTCT CGTCCTGGTG GAGCAGTGCG TGTATACAGC 
GAGGACGCTG TGGACGGTGG GGTGACCGGC TCATACGTCC CGTACTGCTC CATGGCACAT 
GCTCAGCTCT GCTTCCATGG CCACAGGGAC GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT 
CAGGTTATTG TGCCTGGAGC TGAGGGTGTG ACTGAGAGCT CTGAGTCTTC AGATGGAAAG 
AGCATGTTGA TCATGAGTGG AGGAGAGGGA TACATCGACT TCAGAGTGGG TGATGAGGAT 
GGAGGATTGG GACGAGATGA AGATGAAGAA TCAGCTGAAC CCACACTGAA ACTCCAGTCG 
GCTCCTGCCA AACCTGAGCG CAGTTATGTT ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209856.1
CDS	579..4538
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354367.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354367.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCT GTAGGTCAGT GTGGATATCT AGGTCAGAGG 
GCTAATAAGA CGGTGGGAGC GATTGCCTCT CGTCCTGGTG GAGCAGTGCG TGTATACAGC 
GAGGACGCTG TGGACGGTGG GGTGACCGGC TCATACGTCC CGTACTGCTC CATGGCACAT 
GCTCAGCTCT GCTTCCATGG CCACAGGGAC GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT 
CAGGTTATTG TGCCTGGAGC TGAGGGTGTG ACTGAGAGCT CTGAGTCTTC AGATGGAAAG 
AGCATGTTGA TCATGAGTGG AGGAGAGGGA TACATCGACT TCAGAGTGGG TGATGAGGAT 
GGAGGATTGG GACGAGATGA AGATGAAGAA TCAGCTGAAC CCACACTGAA ACTCCAGTCG 
GCTCCTGCCA AACCTGAGCG CAGTTATGTT ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354368.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209857.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa58057
Clone ID Related Accession (Same CDS sequence)	XM_017354368.1
Accession Version	XM_017354368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGCATG CGCTTTCACA CCCGAGTCTG TCCGGCATGA GTCGTGAGGT GGAGAATCTC 
ATTCAGGAAA ATGTGCAGTT GCTTGAGACA AAAAATGCAT TGAATGTGGT AAAGAATGAT 
CTGATCGCTC GCGTGGACGA GCTCTCCTGC GAGAAGGAGG TCCTTCAGGG GGAACTGGAT 
GCGGTTTTGC AGGCCAAAGC CAAACTAGAG GAGAAATACA AGGAGCTGGA GGAGGAGCTC 
AAAAAAATAA AAACCGAGAC AGAGGAGGCC AAACAGAACA ATAATAAAGA AGAAGATGAG 
AGTGATGTGC CCACTGCTCA GAGACGGAGG TTCACGCGGG TGGAAATGGC TCGAGTGCTG 
ATGGAGAGAA ACCAGTACAA GGAGAGACTG ATGGAGCTGC AGGAGGCCGT CCGGTGGACC 
GAAATGATTC GAGCTTCAAG AGAAAATTCA GAAGTCGCAG AAAAGAAGAG GTCAAGCATC 
AGGCAATTCT TCAGTCGCCT CTTCAGTTCT TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC 
AGAGCTTTAC CGTCCAACGT CAAGTACAAC CCACCCAGCA TCATGAAGAA GAGCAACACC 
TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA GCTTTCGACT TCCTCAAAGA AGAGGTGGAC 
TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG AAGCGGGCTC AGTATCAGCA TGTGAAAGCA 
CACATGCAGA AAGAGGACGG CAGGGTGACC GCACATGGCT GGAGTCTGCC GGGAAAATAC 
AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC AAAATAAACC TGCCGGTTCC TGTGTGTCTC 
AAGCCACTGG AGCAGAAAGA TGCTTCTATG AAGCTGCTGT GTGCCACTGG AGTGAATCTC 
TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT GCTGAAGACT CCACAGTAGA GACAGACTCA 
GCCAAACAGA GGAAAGGCTC TCAGAGCAGC CTAGATCAGC TGGAGCACGA TCCTAAGGAG 
CGGGAGCAGA CGCTGCATGT GGAAATGTCC AGTCGCGTCT GGATCTGCAC GAGCACTCAC 
TCCTCTACTA AGGTGATGGT CATAGATGTG ACGCGACCCG ACGACCTCCT GGACAGCTTC 
TACGTCTGTA ACTCATGCGT GCTGTGTATC GCCAGCATAC CAGGAGTGTT GGAGACAGAT 
TATCCTGCGG GGGAGGAGAT TCCTCAGGAT CTGGAGGCGG GACCAGACGC TCTATTGGCC 
AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG TTGAGTGACG TGTCGGCGGT TGGCTGTGGT 
GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG GGCAGCACAG GAGCCCACGA ACAGTCTGCT 
GAACAGAGTC CCGCCAGAGG AAGTGATCTC TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT 
GCCGCTGAGG AGGCTATGGA GGCCACTGAG GAGGACCAGG AGAGTCCGGG ACAGGACAGC 
AGTCAGCCGG GTATCTACAC GGAGCATGTG TTTACAGACC CGCTGGGACC CGAGTCTTCT 
GGGGCGTCAG CAGGCCATGA TAGCCAGAGA GAGTCCGATC GGGACAGGGA TGACACGAGC 
TCAGCAGGGG GCAAGACGGA TCTGGCAAAA GAAGAAACGC AGCGGATGAG CAGTGCACTG 
CCCACCATGT GGCTGGGGGC TCAGAACGGC TGTTTATATG TGCACTCATC TGTTGCTCGA 
TGGAGGAAGT GTCTTCATGC TGTCAAGCTA AAGGATTCCA TCTTGAACAT TGTACATGTG 
AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC GGGACACTGG CCATCTTCCA TAGAGGTCTT 
GACAGCCAGT GGGATTTAAC CAACTACCAT CTTCTGGACC TGGGCCGGCC GCATCACTCA 
ATCCGCTGCA TGACTGTGGT TCACGATAAA GTGTGGTGCG GCTACAGGAA CAAGATCTAC 
ATCATTCAGC CCAAAGCTAT GAAAATCGAG AGGTCTTTTG ATGCCCATCC GCGTAAGGAG 
AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT GACGGGATCT GGGTGTCCAT CCGGCTGGAC 
TCAACGCTTC GCCTATTCCA TGCTCACACC TTCCAGCATC TGCAGGACGT AGACATTGAG 
CCTTACGTCA GCAAAATGTT AGGAACTGGA AAACTGGGCT TCTCTTTTGT GAGAATCACT 
GCTCTGATGG TTTCTTGTAA TCGGCTGTGG GTGGGAACAG GAAATGGTGT CATCATCTCC 
ATCCCTCTGT CAGACTCTGT AGGTCAGTGT GGATATCTAG GTCAGAGGGC AGCTAATAAG 
ACGGTGGGAG CGATTGCCTC TCGTCCTGGT GGAGCAGTGC GTGTATACAG CGAGGACGCT 
GTGGACGGTG GGGTGACCGG CTCATACGTC CCGTACTGCT CCATGGCACA TGCTCAGCTC 
TGCTTCCATG GCCACAGGGA CGCTGTTAAA TTTTTCGCTA CAGTTCCAGG TCAGGTTATT 
GTGCCTGGAG CTGAGGGTGT GACTGAGAGC TCTGAGTCTT CAGATGGAAA GAGCATGTTG 
ATCATGAGTG GAGGAGAGGG ATACATCGAC TTCAGAGTGG GTGATGAGGA TGGAGGATTG 
GGACGAGATG AAGATGAAGA ATCAGCTGAA CCCACACTGA AACTCCAGTC GGCTCCTGCC 
AAACCTGAGC GCAGTTATGT TATAGTCTGG CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209857.1
CDS	579..4529
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354368.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354368.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGCATG CGCTTTCACA CCCGAGTCTG TCCGGCATGA GTCGTGAGGT GGAGAATCTC 
ATTCAGGAAA ATGTGCAGTT GCTTGAGACA AAAAATGCAT TGAATGTGGT AAAGAATGAT 
CTGATCGCTC GCGTGGACGA GCTCTCCTGC GAGAAGGAGG TCCTTCAGGG GGAACTGGAT 
GCGGTTTTGC AGGCCAAAGC CAAACTAGAG GAGAAATACA AGGAGCTGGA GGAGGAGCTC 
AAAAAAATAA AAACCGAGAC AGAGGAGGCC AAACAGAACA ATAATAAAGA AGAAGATGAG 
AGTGATGTGC CCACTGCTCA GAGACGGAGG TTCACGCGGG TGGAAATGGC TCGAGTGCTG 
ATGGAGAGAA ACCAGTACAA GGAGAGACTG ATGGAGCTGC AGGAGGCCGT CCGGTGGACC 
GAAATGATTC GAGCTTCAAG AGAAAATTCA GAAGTCGCAG AAAAGAAGAG GTCAAGCATC 
AGGCAATTCT TCAGTCGCCT CTTCAGTTCT TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC 
AGAGCTTTAC CGTCCAACGT CAAGTACAAC CCACCCAGCA TCATGAAGAA GAGCAACACC 
TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA GCTTTCGACT TCCTCAAAGA AGAGGTGGAC 
TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG AAGCGGGCTC AGTATCAGCA TGTGAAAGCA 
CACATGCAGA AAGAGGACGG CAGGGTGACC GCACATGGCT GGAGTCTGCC GGGAAAATAC 
AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC AAAATAAACC TGCCGGTTCC TGTGTGTCTC 
AAGCCACTGG AGCAGAAAGA TGCTTCTATG AAGCTGCTGT GTGCCACTGG AGTGAATCTC 
TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT GCTGAAGACT CCACAGTAGA GACAGACTCA 
GCCAAACAGA GGAAAGGCTC TCAGAGCAGC CTAGATCAGC TGGAGCACGA TCCTAAGGAG 
CGGGAGCAGA CGCTGCATGT GGAAATGTCC AGTCGCGTCT GGATCTGCAC GAGCACTCAC 
TCCTCTACTA AGGTGATGGT CATAGATGTG ACGCGACCCG ACGACCTCCT GGACAGCTTC 
TACGTCTGTA ACTCATGCGT GCTGTGTATC GCCAGCATAC CAGGAGTGTT GGAGACAGAT 
TATCCTGCGG GGGAGGAGAT TCCTCAGGAT CTGGAGGCGG GACCAGACGC TCTATTGGCC 
AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG TTGAGTGACG TGTCGGCGGT TGGCTGTGGT 
GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG GGCAGCACAG GAGCCCACGA ACAGTCTGCT 
GAACAGAGTC CCGCCAGAGG AAGTGATCTC TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT 
GCCGCTGAGG AGGCTATGGA GGCCACTGAG GAGGACCAGG AGAGTCCGGG ACAGGACAGC 
AGTCAGCCGG GTATCTACAC GGAGCATGTG TTTACAGACC CGCTGGGACC CGAGTCTTCT 
GGGGCGTCAG CAGGCCATGA TAGCCAGAGA GAGTCCGATC GGGACAGGGA TGACACGAGC 
TCAGCAGGGG GCAAGACGGA TCTGGCAAAA GAAGAAACGC AGCGGATGAG CAGTGCACTG 
CCCACCATGT GGCTGGGGGC TCAGAACGGC TGTTTATATG TGCACTCATC TGTTGCTCGA 
TGGAGGAAGT GTCTTCATGC TGTCAAGCTA AAGGATTCCA TCTTGAACAT TGTACATGTG 
AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC GGGACACTGG CCATCTTCCA TAGAGGTCTT 
GACAGCCAGT GGGATTTAAC CAACTACCAT CTTCTGGACC TGGGCCGGCC GCATCACTCA 
ATCCGCTGCA TGACTGTGGT TCACGATAAA GTGTGGTGCG GCTACAGGAA CAAGATCTAC 
ATCATTCAGC CCAAAGCTAT GAAAATCGAG AGGTCTTTTG ATGCCCATCC GCGTAAGGAG 
AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT GACGGGATCT GGGTGTCCAT CCGGCTGGAC 
TCAACGCTTC GCCTATTCCA TGCTCACACC TTCCAGCATC TGCAGGACGT AGACATTGAG 
CCTTACGTCA GCAAAATGTT AGGAACTGGA AAACTGGGCT TCTCTTTTGT GAGAATCACT 
GCTCTGATGG TTTCTTGTAA TCGGCTGTGG GTGGGAACAG GAAATGGTGT CATCATCTCC 
ATCCCTCTGT CAGACTCTGT AGGTCAGTGT GGATATCTAG GTCAGAGGGC AGCTAATAAG 
ACGGTGGGAG CGATTGCCTC TCGTCCTGGT GGAGCAGTGC GTGTATACAG CGAGGACGCT 
GTGGACGGTG GGGTGACCGG CTCATACGTC CCGTACTGCT CCATGGCACA TGCTCAGCTC 
TGCTTCCATG GCCACAGGGA CGCTGTTAAA TTTTTCGCTA CAGTTCCAGG TCAGGTTATT 
GTGCCTGGAG CTGAGGGTGT GACTGAGAGC TCTGAGTCTT CAGATGGAAA GAGCATGTTG 
ATCATGAGTG GAGGAGAGGG ATACATCGAC TTCAGAGTGG GTGATGAGGA TGGAGGATTG 
GGACGAGATG AAGATGAAGA ATCAGCTGAA CCCACACTGA AACTCCAGTC GGCTCCTGCC 
AAACCTGAGC GCAGTTATGT TATAGTCTGG CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354369.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209858.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X4, mRNA.

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CloneID	ODa58058
Clone ID Related Accession (Same CDS sequence)	XM_017354369.1 , XM_017354369.2
Accession Version	XM_017354369.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
GTAGGTCAGT GTGGATATCT AGGTCAGAGG GCAGCTAATA AGACGGTGGG AGCGATTGCC 
TCTCGTCCTG GTGGAGCAGT GCGTGTATAC AGCGAGGACG CTGTGGACGG TGGGGTGACC 
GGCTCATACG TCCCGTACTG CTCCATGGCA CATGCTCAGC TCTGCTTCCA TGGCCACAGG 
GACGCTGTTA AATTTTTCGC TACAGTTCCA GGTCAGGTTA TTGTGCCTGG AGCTGAGGGT 
GTGACTGAGA GCTCTGAGTC TTCAGATGGA AAGAGCATGT TGATCATGAG TGGAGGAGAG 
GGATACATCG ACTTCAGAGT GGGTGATGAG GATGGAGGAT TGGGACGAGA TGAAGATGAA 
GAATCAGCTG AACCCACACT GAAACTCCAG TCGGCTCCTG CCAAACCTGA GCGCAGTTAT 
GTTATAGTCT GGCAGGTTGC GAGTGACCGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209858.1
CDS	579..4511
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354369.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354369.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
GTAGGTCAGT GTGGATATCT AGGTCAGAGG GCAGCTAATA AGACGGTGGG AGCGATTGCC 
TCTCGTCCTG GTGGAGCAGT GCGTGTATAC AGCGAGGACG CTGTGGACGG TGGGGTGACC 
GGCTCATACG TCCCGTACTG CTCCATGGCA CATGCTCAGC TCTGCTTCCA TGGCCACAGG 
GACGCTGTTA AATTTTTCGC TACAGTTCCA GGTCAGGTTA TTGTGCCTGG AGCTGAGGGT 
GTGACTGAGA GCTCTGAGTC TTCAGATGGA AAGAGCATGT TGATCATGAG TGGAGGAGAG 
GGATACATCG ACTTCAGAGT GGGTGATGAG GATGGAGGAT TGGGACGAGA TGAAGATGAA 
GAATCAGCTG AACCCACACT GAAACTCCAG TCGGCTCCTG CCAAACCTGA GCGCAGTTAT 
GTTATAGTCT GGCAGGTTGC GAGTGACCGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354369.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209858.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58058
Clone ID Related Accession (Same CDS sequence)	XM_017354369.1 , XM_017354369.2
Accession Version	XM_017354369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
GTAGGTCAGT GTGGATATCT AGGTCAGAGG GCAGCTAATA AGACGGTGGG AGCGATTGCC 
TCTCGTCCTG GTGGAGCAGT GCGTGTATAC AGCGAGGACG CTGTGGACGG TGGGGTGACC 
GGCTCATACG TCCCGTACTG CTCCATGGCA CATGCTCAGC TCTGCTTCCA TGGCCACAGG 
GACGCTGTTA AATTTTTCGC TACAGTTCCA GGTCAGGTTA TTGTGCCTGG AGCTGAGGGT 
GTGACTGAGA GCTCTGAGTC TTCAGATGGA AAGAGCATGT TGATCATGAG TGGAGGAGAG 
GGATACATCG ACTTCAGAGT GGGTGATGAG GATGGAGGAT TGGGACGAGA TGAAGATGAA 
GAATCAGCTG AACCCACACT GAAACTCCAG TCGGCTCCTG CCAAACCTGA GCGCAGTTAT 
GTTATAGTCT GGCAGGTTGC GAGTGACCGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209858.1
CDS	579..4511
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354369.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354369.2

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGGCAT GAGTCGTGAG GTGGAGAATC TCATTCAGGA AAATGTGCAG 
TTGCTTGAGA CAAAAAATGC ATTGAATGTG GTAAAGAATG ATCTGATCGC TCGCGTGGAC 
GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG GGGGAACTGG ATGCGGTTTT GCAGGCCAAA 
GCCAAACTAG AGGAGAAATA CAAGGAGCTG GAGGAGGAGC TCAAAAAAAT AAAAACCGAG 
ACAGAGGAGG CCAAACAGAA CAATAATAAA GAAGAAGATG AGAGTGATGT GCCCACTGCT 
CAGAGACGGA GGTTCACGCG GGTGGAAATG GCTCGAGTGC TGATGGAGAG AAACCAGTAC 
AAGGAGAGAC TGATGGAGCT GCAGGAGGCC GTCCGGTGGA CCGAAATGAT TCGAGCTTCA 
AGAGAAAATT CAGAAGTCGC AGAAAAGAAG AGGTCAAGCA TCAGGCAATT CTTCAGTCGC 
CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC 
GTCAAGTACA ACCCACCCAG CATCATGAAG AAGAGCAACA CCTTCTCCCA GTTCCCCAGA 
GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC 
CGCCGGGAGC AGAAGCGGGC TCAGTATCAG CATGTGAAAG CACACATGCA GAAAGAGGAC 
GGCAGGGTGA CCGCACATGG CTGGAGTCTG CCGGGAAAAT ACAAGGTTGC CAATGGTGGC 
CCTGTAGAGA ACAAAATAAA CCTGCCGGTT CCTGTGTGTC TCAAGCCACT GGAGCAGAAA 
GATGCTTCTA TGAAGCTGCT GTGTGCCACT GGAGTGAATC TCTCTGGAGG ACGCTTGTGT 
GACAGAGGCT CTGCTGAAGA CTCCACAGTA GAGACAGACT CAGCCAAACA GAGGAAAGGC 
TCTCAGAGCA GCCTAGATCA GCTGGAGCAC GATCCTAAGG AGCGGGAGCA GACGCTGCAT 
GTGGAAATGT CCAGTCGCGT CTGGATCTGC ACGAGCACTC ACTCCTCTAC TAAGGTGATG 
GTCATAGATG TGACGCGACC CGACGACCTC CTGGACAGCT TCTACGTCTG TAACTCATGC 
GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG TTGGAGACAG ATTATCCTGC GGGGGAGGAG 
ATTCCTCAGG ATCTGGAGGC GGGACCAGAC GCTCTATTGG CCAGCAGCAG CTCTCAGGGC 
AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA 
GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC GAACAGTCTG CTGAACAGAG TCCCGCCAGA 
GGAAGTGATC TCTCTAGAGC TCCCAGTGAA GATGGTCTCC CTGCCGCTGA GGAGGCTATG 
GAGGCCACTG AGGAGGACCA GGAGAGTCCG GGACAGGACA GCAGTCAGCC GGGTATCTAC 
ACGGAGCATG TGTTTACAGA CCCGCTGGGA CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT 
GATAGCCAGA GAGAGTCCGA TCGGGACAGG GATGACACGA GCTCAGCAGG GGGCAAGACG 
GATCTGGCAA AAGAAGAAAC GCAGCGGATG AGCAGTGCAC TGCCCACCAT GTGGCTGGGG 
GCTCAGAACG GCTGTTTATA TGTGCACTCA TCTGTTGCTC GATGGAGGAA GTGTCTTCAT 
GCTGTCAAGC TAAAGGATTC CATCTTGAAC ATTGTACATG TGAAAGGCCG TGTTTTGGTG 
GCTCTAGCTG ACGGGACACT GGCCATCTTC CATAGAGGTC TTGACAGCCA GTGGGATTTA 
ACCAACTACC ATCTTCTGGA CCTGGGCCGG CCGCATCACT CAATCCGCTG CATGACTGTG 
GTTCACGATA AAGTGTGGTG CGGCTACAGG AACAAGATCT ACATCATTCA GCCCAAAGCT 
ATGAAAATCG AGAGGTCTTT TGATGCCCAT CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG 
GCCTGGGTGG GTGACGGGAT CTGGGTGTCC ATCCGGCTGG ACTCAACGCT TCGCCTATTC 
CATGCTCACA CCTTCCAGCA TCTGCAGGAC GTAGACATTG AGCCTTACGT CAGCAAAATG 
TTAGGAACTG GAAAACTGGG CTTCTCTTTT GTGAGAATCA CTGCTCTGAT GGTTTCTTGT 
AATCGGCTGT GGGTGGGAAC AGGAAATGGT GTCATCATCT CCATCCCTCT GTCAGACTCT 
GTAGGTCAGT GTGGATATCT AGGTCAGAGG GCAGCTAATA AGACGGTGGG AGCGATTGCC 
TCTCGTCCTG GTGGAGCAGT GCGTGTATAC AGCGAGGACG CTGTGGACGG TGGGGTGACC 
GGCTCATACG TCCCGTACTG CTCCATGGCA CATGCTCAGC TCTGCTTCCA TGGCCACAGG 
GACGCTGTTA AATTTTTCGC TACAGTTCCA GGTCAGGTTA TTGTGCCTGG AGCTGAGGGT 
GTGACTGAGA GCTCTGAGTC TTCAGATGGA AAGAGCATGT TGATCATGAG TGGAGGAGAG 
GGATACATCG ACTTCAGAGT GGGTGATGAG GATGGAGGAT TGGGACGAGA TGAAGATGAA 
GAATCAGCTG AACCCACACT GAAACTCCAG TCGGCTCCTG CCAAACCTGA GCGCAGTTAT 
GTTATAGTCT GGCAGGTTGC GAGTGACCGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354371.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209860.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa58059
Clone ID Related Accession (Same CDS sequence)	XM_017354371.1
Accession Version	XM_017354371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCA GCTAATAAGA CGGTGGGAGC GATTGCCTCT 
CGTCCTGGTG GAGCAGTGCG TGTATACAGC GAGGACGCTG TGGACGGTGG GGTGACCGGC 
TCATACGTCC CGTACTGCTC CATGGCACAT GCTCAGCTCT GCTTCCATGG CCACAGGGAC 
GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT CAGGTTATTG TGCCTGGAGC TGAGGGTGTG 
ACTGAGAGCT CTGAGTCTTC AGATGGAAAG AGCATGTTGA TCATGAGTGG AGGAGAGGGA 
TACATCGACT TCAGAGTGGG TGATGAGGAT GGAGGATTGG GACGAGATGA AGATGAAGAA 
TCAGCTGAAC CCACACTGAA ACTCCAGTCG GCTCCTGCCA AACCTGAGCG CAGTTATGTT 
ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209860.1
CDS	579..4508
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354371.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354371.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCA GCTAATAAGA CGGTGGGAGC GATTGCCTCT 
CGTCCTGGTG GAGCAGTGCG TGTATACAGC GAGGACGCTG TGGACGGTGG GGTGACCGGC 
TCATACGTCC CGTACTGCTC CATGGCACAT GCTCAGCTCT GCTTCCATGG CCACAGGGAC 
GCTGTTAAAT TTTTCGCTAC AGTTCCAGGT CAGGTTATTG TGCCTGGAGC TGAGGGTGTG 
ACTGAGAGCT CTGAGTCTTC AGATGGAAAG AGCATGTTGA TCATGAGTGG AGGAGAGGGA 
TACATCGACT TCAGAGTGGG TGATGAGGAT GGAGGATTGG GACGAGATGA AGATGAAGAA 
TCAGCTGAAC CCACACTGAA ACTCCAGTCG GCTCCTGCCA AACCTGAGCG CAGTTATGTT 
ATAGTCTGGC AGGTTGCGAG TGACCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354372.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209861.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X6, mRNA.

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CloneID	ODa58060
Clone ID Related Accession (Same CDS sequence)	XM_017354372.1
Accession Version	XM_017354372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCT AATAAGACGG TGGGAGCGAT TGCCTCTCGT 
CCTGGTGGAG CAGTGCGTGT ATACAGCGAG GACGCTGTGG ACGGTGGGGT GACCGGCTCA 
TACGTCCCGT ACTGCTCCAT GGCACATGCT CAGCTCTGCT TCCATGGCCA CAGGGACGCT 
GTTAAATTTT TCGCTACAGT TCCAGGTCAG GTTATTGTGC CTGGAGCTGA GGGTGTGACT 
GAGAGCTCTG AGTCTTCAGA TGGAAAGAGC ATGTTGATCA TGAGTGGAGG AGAGGGATAC 
ATCGACTTCA GAGTGGGTGA TGAGGATGGA GGATTGGGAC GAGATGAAGA TGAAGAATCA 
GCTGAACCCA CACTGAAACT CCAGTCGGCT CCTGCCAAAC CTGAGCGCAG TTATGTTATA 
GTCTGGCAGG TTGCGAGTGA CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209861.1
CDS	579..4505
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSNKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354372.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354372.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGTGGAGT ACTCTGTGCA TGCGCTTTCA CACCCGAGTC TGTCCGGCAT GAGTCGTGAG 
GTGGAGAATC TCATTCAGGA AAATGTGCAG TTGCTTGAGA CAAAAAATGC ATTGAATGTG 
GTAAAGAATG ATCTGATCGC TCGCGTGGAC GAGCTCTCCT GCGAGAAGGA GGTCCTTCAG 
GGGGAACTGG ATGCGGTTTT GCAGGCCAAA GCCAAACTAG AGGAGAAATA CAAGGAGCTG 
GAGGAGGAGC TCAAAAAAAT AAAAACCGAG ACAGAGGAGG CCAAACAGAA CAATAATAAA 
GAAGAAGATG AGAGTGATGT GCCCACTGCT CAGAGACGGA GGTTCACGCG GGTGGAAATG 
GCTCGAGTGC TGATGGAGAG AAACCAGTAC AAGGAGAGAC TGATGGAGCT GCAGGAGGCC 
GTCCGGTGGA CCGAAATGAT TCGAGCTTCA AGAGAAAATT CAGAAGTCGC AGAAAAGAAG 
AGGTCAAGCA TCAGGCAATT CTTCAGTCGC CTCTTCAGTT CTTCCAGTTC TGCTGCGTCC 
AGCAGCTCGT CCAGAGCTTT ACCGTCCAAC GTCAAGTACA ACCCACCCAG CATCATGAAG 
AAGAGCAACA CCTTCTCCCA GTTCCCCAGA GAAAAGTCCA AAGCTTTCGA CTTCCTCAAA 
GAAGAGGTGG ACTCGAGTGG CTCTCTGTCC CGCCGGGAGC AGAAGCGGGC TCAGTATCAG 
CATGTGAAAG CACACATGCA GAAAGAGGAC GGCAGGGTGA CCGCACATGG CTGGAGTCTG 
CCGGGAAAAT ACAAGGTTGC CAATGGTGGC CCTGTAGAGA ACAAAATAAA CCTGCCGGTT 
CCTGTGTGTC TCAAGCCACT GGAGCAGAAA GATGCTTCTA TGAAGCTGCT GTGTGCCACT 
GGAGTGAATC TCTCTGGAGG ACGCTTGTGT GACAGAGGCT CTGCTGAAGA CTCCACAGTA 
GAGACAGACT CAGCCAAACA GAGGAAAGGC TCTCAGAGCA GCCTAGATCA GCTGGAGCAC 
GATCCTAAGG AGCGGGAGCA GACGCTGCAT GTGGAAATGT CCAGTCGCGT CTGGATCTGC 
ACGAGCACTC ACTCCTCTAC TAAGGTGATG GTCATAGATG TGACGCGACC CGACGACCTC 
CTGGACAGCT TCTACGTCTG TAACTCATGC GTGCTGTGTA TCGCCAGCAT ACCAGGAGTG 
TTGGAGACAG ATTATCCTGC GGGGGAGGAG ATTCCTCAGG ATCTGGAGGC GGGACCAGAC 
GCTCTATTGG CCAGCAGCAG CTCTCAGGGC AGTGAGGGAG TGTTGAGTGA CGTGTCGGCG 
GTTGGCTGTG GTGTTGAGGA GTCCAGTTTA GGCTCCCAAG CGGGCAGCAC AGGAGCCCAC 
GAACAGTCTG CTGAACAGAG TCCCGCCAGA GGAAGTGATC TCTCTAGAGC TCCCAGTGAA 
GATGGTCTCC CTGCCGCTGA GGAGGCTATG GAGGCCACTG AGGAGGACCA GGAGAGTCCG 
GGACAGGACA GCAGTCAGCC GGGTATCTAC ACGGAGCATG TGTTTACAGA CCCGCTGGGA 
CCCGAGTCTT CTGGGGCGTC AGCAGGCCAT GATAGCCAGA GAGAGTCCGA TCGGGACAGG 
GATGACACGA GCTCAGCAGG GGGCAAGACG GATCTGGCAA AAGAAGAAAC GCAGCGGATG 
AGCAGTGCAC TGCCCACCAT GTGGCTGGGG GCTCAGAACG GCTGTTTATA TGTGCACTCA 
TCTGTTGCTC GATGGAGGAA GTGTCTTCAT GCTGTCAAGC TAAAGGATTC CATCTTGAAC 
ATTGTACATG TGAAAGGCCG TGTTTTGGTG GCTCTAGCTG ACGGGACACT GGCCATCTTC 
CATAGAGGTC TTGACAGCCA GTGGGATTTA ACCAACTACC ATCTTCTGGA CCTGGGCCGG 
CCGCATCACT CAATCCGCTG CATGACTGTG GTTCACGATA AAGTGTGGTG CGGCTACAGG 
AACAAGATCT ACATCATTCA GCCCAAAGCT ATGAAAATCG AGAGGTCTTT TGATGCCCAT 
CCGCGTAAGG AGAGTCAGGT GCGTCAGCTG GCCTGGGTGG GTGACGGGAT CTGGGTGTCC 
ATCCGGCTGG ACTCAACGCT TCGCCTATTC CATGCTCACA CCTTCCAGCA TCTGCAGGAC 
GTAGACATTG AGCCTTACGT CAGCAAAATG TTAGGAACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATCA CTGCTCTGAT GGTTTCTTGT AATCGGCTGT GGGTGGGAAC AGGAAATGGT 
GTCATCATCT CCATCCCTCT GTCAGACTCT AATAAGACGG TGGGAGCGAT TGCCTCTCGT 
CCTGGTGGAG CAGTGCGTGT ATACAGCGAG GACGCTGTGG ACGGTGGGGT GACCGGCTCA 
TACGTCCCGT ACTGCTCCAT GGCACATGCT CAGCTCTGCT TCCATGGCCA CAGGGACGCT 
GTTAAATTTT TCGCTACAGT TCCAGGTCAG GTTATTGTGC CTGGAGCTGA GGGTGTGACT 
GAGAGCTCTG AGTCTTCAGA TGGAAAGAGC ATGTTGATCA TGAGTGGAGG AGAGGGATAC 
ATCGACTTCA GAGTGGGTGA TGAGGATGGA GGATTGGGAC GAGATGAAGA TGAAGAATCA 
GCTGAACCCA CACTGAAACT CCAGTCGGCT CCTGCCAAAC CTGAGCGCAG TTATGTTATA 
GTCTGGCAGG TTGCGAGTGA CCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354374.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209863.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa58061
Clone ID Related Accession (Same CDS sequence)	XM_017354374.2 , XM_017354374.1
Accession Version	XM_017354374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGGCATGA GTCGTGAGGT GGAGAATCTC ATTCAGGAAA ATGTGCAGTT GCTTGAGACA 
AAAAATGCAT TGAATGTGGT AAAGAATGAT CTGATCGCTC GCGTGGACGA GCTCTCCTGC 
GAGAAGGAGG TCCTTCAGGG GGAACTGGAT GCGGTTTTGC AGGCCAAAGC CAAACTAGAG 
GAGAAATACA AGGAGCTGGA GGAGGAGCTC AAAAAAATAA AAACCGAGAC AGAGGAGGCC 
AAACAGAACA ATAATAAAGA AGAAGATGAG AGTGATGTGC CCACTGCTCA GAGACGGAGG 
TTCACGCGGG TGGAAATGGC TCGAGTGCTG ATGGAGAGAA ACCAGTACAA GGAGAGACTG 
ATGGAGCTGC AGGAGGCCGT CCGGTGGACC GAAATGATTC GAGCTTCAAG AGAAAATTCA 
GAAGTCGCAG AAAAGAAGAG GTCAAGCATC AGGCAATTCT TCAGTCGCCT CTTCAGTTCT 
TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC AGAGCTTTAC CGTCCAACGT CAAGTACAAC 
CCACCCAGCA TCATGAAGAA GAGCAACACC TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA 
GCTTTCGACT TCCTCAAAGA AGAGGTGGAC TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG 
AAGCGGGCTC AGTATCAGCA TGTGAAAGCA CACATGCAGA AAGAGGACGG CAGGGTGACC 
GCACATGGCT GGAGTCTGCC GGGAAAATAC AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC 
AAAATAAACC TGCCGGTTCC TGTGTGTCTC AAGCCACTGG AGCAGAAAGA TGCTTCTATG 
AAGCTGCTGT GTGCCACTGG AGTGAATCTC TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT 
GCTGAAGACT CCACAGTAGA GACAGACTCA GCCAAACAGA GGAAAGGCTC TCAGAGCAGC 
CTAGATCAGC TGGAGCACGA TCCTAAGGAG CGGGAGCAGA CGCTGCATGT GGAAATGTCC 
AGTCGCGTCT GGATCTGCAC GAGCACTCAC TCCTCTACTA AGGTGATGGT CATAGATGTG 
ACGCGACCCG ACGACCTCCT GGACAGCTTC TACGTCTGTA ACTCATGCGT GCTGTGTATC 
GCCAGCATAC CAGGAGTGTT GGAGACAGAT TATCCTGCGG GGGAGGAGAT TCCTCAGGAT 
CTGGAGGCGG GACCAGACGC TCTATTGGCC AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG 
TTGAGTGACG TGTCGGCGGT TGGCTGTGGT GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG 
GGCAGCACAG GAGCCCACGA ACAGTCTGCT GAACAGAGTC CCGCCAGAGG AAGTGATCTC 
TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT GCCGCTGAGG AGGCTATGGA GGCCACTGAG 
GAGGACCAGG AGAGTCCGGG ACAGGACAGC AGTCAGCCGG GTATCTACAC GGAGCATGTG 
TTTACAGACC CGCTGGGACC CGAGTCTTCT GGGGCGTCAG CAGGCCATGA TAGCCAGAGA 
GAGTCCGATC GGGACAGGGA TGACACGAGC TCAGCAGGGG GCAAGACGGA TCTGGCAAAA 
GAAGAAACGC AGCGGATGAG CAGTGCACTG CCCACCATGT GGCTGGGGGC TCAGAACGGC 
TGTTTATATG TGCACTCATC TGTTGCTCGA TGGAGGAAGT GTCTTCATGC TGTCAAGCTA 
AAGGATTCCA TCTTGAACAT TGTACATGTG AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC 
GGGACACTGG CCATCTTCCA TAGAGGTCTT GACAGCCAGT GGGATTTAAC CAACTACCAT 
CTTCTGGACC TGGGCCGGCC GCATCACTCA ATCCGCTGCA TGACTGTGGT TCACGATAAA 
GTGTGGTGCG GCTACAGGAA CAAGATCTAC ATCATTCAGC CCAAAGCTAT GAAAATCGAG 
AGGTCTTTTG ATGCCCATCC GCGTAAGGAG AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT 
GACGGGATCT GGGTGTCCAT CCGGCTGGAC TCAACGCTTC GCCTATTCCA TGCTCACACC 
TTCCAGCATC TGCAGGACGT AGACATTGAG CCTTACGTCA GCAAAATGTT AGGAACTGGA 
AAACTGGGCT TCTCTTTTGT GAGAATCACT GCTCTGATGG TTTCTTGTAA TCGGCTGTGG 
GTGGGAACAG GAAATGGTGT CATCATCTCC ATCCCTCTGT CAGACTCTGT AGGTCAGTGT 
GGATATCTAG GTCAGAGGGC AGCTAATAAG ACGGTGGGAG CGATTGCCTC TCGTCCTGGT 
GGAGCAGTGC GTGTATACAG CGAGGACGCT GTGGACGGTG GGGTGACCGG CTCATACGTC 
CCGTACTGCT CCATGGCACA TGCTCAGCTC TGCTTCCATG GCCACAGGGA CGCTGTTAAA 
TTTTTCGCTA CAGTTCCAGG TCAGGTTATT GTGCCTGGAG CTGAGGGTGT GACTGAGAGC 
TCTGAGTCTT CAGATGGAAA GAGCATGTTG ATCATGAGTG GAGGAGAGGG ATACATCGAC 
TTCAGAGTGG GTGATGAGGA TGGAGGATTG GGACGAGATG AAGATGAAGA ATCAGCTGAA 
CCCACACTGA AACTCCAGTC GGCTCCTGCC AAACCTGAGC GCAGTTATGT TATAGTCTGG 
CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209863.1
CDS	579..4499
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354374.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354374.2

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGGCATGA GTCGTGAGGT GGAGAATCTC ATTCAGGAAA ATGTGCAGTT GCTTGAGACA 
AAAAATGCAT TGAATGTGGT AAAGAATGAT CTGATCGCTC GCGTGGACGA GCTCTCCTGC 
GAGAAGGAGG TCCTTCAGGG GGAACTGGAT GCGGTTTTGC AGGCCAAAGC CAAACTAGAG 
GAGAAATACA AGGAGCTGGA GGAGGAGCTC AAAAAAATAA AAACCGAGAC AGAGGAGGCC 
AAACAGAACA ATAATAAAGA AGAAGATGAG AGTGATGTGC CCACTGCTCA GAGACGGAGG 
TTCACGCGGG TGGAAATGGC TCGAGTGCTG ATGGAGAGAA ACCAGTACAA GGAGAGACTG 
ATGGAGCTGC AGGAGGCCGT CCGGTGGACC GAAATGATTC GAGCTTCAAG AGAAAATTCA 
GAAGTCGCAG AAAAGAAGAG GTCAAGCATC AGGCAATTCT TCAGTCGCCT CTTCAGTTCT 
TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC AGAGCTTTAC CGTCCAACGT CAAGTACAAC 
CCACCCAGCA TCATGAAGAA GAGCAACACC TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA 
GCTTTCGACT TCCTCAAAGA AGAGGTGGAC TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG 
AAGCGGGCTC AGTATCAGCA TGTGAAAGCA CACATGCAGA AAGAGGACGG CAGGGTGACC 
GCACATGGCT GGAGTCTGCC GGGAAAATAC AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC 
AAAATAAACC TGCCGGTTCC TGTGTGTCTC AAGCCACTGG AGCAGAAAGA TGCTTCTATG 
AAGCTGCTGT GTGCCACTGG AGTGAATCTC TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT 
GCTGAAGACT CCACAGTAGA GACAGACTCA GCCAAACAGA GGAAAGGCTC TCAGAGCAGC 
CTAGATCAGC TGGAGCACGA TCCTAAGGAG CGGGAGCAGA CGCTGCATGT GGAAATGTCC 
AGTCGCGTCT GGATCTGCAC GAGCACTCAC TCCTCTACTA AGGTGATGGT CATAGATGTG 
ACGCGACCCG ACGACCTCCT GGACAGCTTC TACGTCTGTA ACTCATGCGT GCTGTGTATC 
GCCAGCATAC CAGGAGTGTT GGAGACAGAT TATCCTGCGG GGGAGGAGAT TCCTCAGGAT 
CTGGAGGCGG GACCAGACGC TCTATTGGCC AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG 
TTGAGTGACG TGTCGGCGGT TGGCTGTGGT GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG 
GGCAGCACAG GAGCCCACGA ACAGTCTGCT GAACAGAGTC CCGCCAGAGG AAGTGATCTC 
TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT GCCGCTGAGG AGGCTATGGA GGCCACTGAG 
GAGGACCAGG AGAGTCCGGG ACAGGACAGC AGTCAGCCGG GTATCTACAC GGAGCATGTG 
TTTACAGACC CGCTGGGACC CGAGTCTTCT GGGGCGTCAG CAGGCCATGA TAGCCAGAGA 
GAGTCCGATC GGGACAGGGA TGACACGAGC TCAGCAGGGG GCAAGACGGA TCTGGCAAAA 
GAAGAAACGC AGCGGATGAG CAGTGCACTG CCCACCATGT GGCTGGGGGC TCAGAACGGC 
TGTTTATATG TGCACTCATC TGTTGCTCGA TGGAGGAAGT GTCTTCATGC TGTCAAGCTA 
AAGGATTCCA TCTTGAACAT TGTACATGTG AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC 
GGGACACTGG CCATCTTCCA TAGAGGTCTT GACAGCCAGT GGGATTTAAC CAACTACCAT 
CTTCTGGACC TGGGCCGGCC GCATCACTCA ATCCGCTGCA TGACTGTGGT TCACGATAAA 
GTGTGGTGCG GCTACAGGAA CAAGATCTAC ATCATTCAGC CCAAAGCTAT GAAAATCGAG 
AGGTCTTTTG ATGCCCATCC GCGTAAGGAG AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT 
GACGGGATCT GGGTGTCCAT CCGGCTGGAC TCAACGCTTC GCCTATTCCA TGCTCACACC 
TTCCAGCATC TGCAGGACGT AGACATTGAG CCTTACGTCA GCAAAATGTT AGGAACTGGA 
AAACTGGGCT TCTCTTTTGT GAGAATCACT GCTCTGATGG TTTCTTGTAA TCGGCTGTGG 
GTGGGAACAG GAAATGGTGT CATCATCTCC ATCCCTCTGT CAGACTCTGT AGGTCAGTGT 
GGATATCTAG GTCAGAGGGC AGCTAATAAG ACGGTGGGAG CGATTGCCTC TCGTCCTGGT 
GGAGCAGTGC GTGTATACAG CGAGGACGCT GTGGACGGTG GGGTGACCGG CTCATACGTC 
CCGTACTGCT CCATGGCACA TGCTCAGCTC TGCTTCCATG GCCACAGGGA CGCTGTTAAA 
TTTTTCGCTA CAGTTCCAGG TCAGGTTATT GTGCCTGGAG CTGAGGGTGT GACTGAGAGC 
TCTGAGTCTT CAGATGGAAA GAGCATGTTG ATCATGAGTG GAGGAGAGGG ATACATCGAC 
TTCAGAGTGG GTGATGAGGA TGGAGGATTG GGACGAGATG AAGATGAAGA ATCAGCTGAA 
CCCACACTGA AACTCCAGTC GGCTCCTGCC AAACCTGAGC GCAGTTATGT TATAGTCTGG 
CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354374.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209863.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa58061
Clone ID Related Accession (Same CDS sequence)	XM_017354374.2 , XM_017354374.1
Accession Version	XM_017354374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGGCATGA GTCGTGAGGT GGAGAATCTC ATTCAGGAAA ATGTGCAGTT GCTTGAGACA 
AAAAATGCAT TGAATGTGGT AAAGAATGAT CTGATCGCTC GCGTGGACGA GCTCTCCTGC 
GAGAAGGAGG TCCTTCAGGG GGAACTGGAT GCGGTTTTGC AGGCCAAAGC CAAACTAGAG 
GAGAAATACA AGGAGCTGGA GGAGGAGCTC AAAAAAATAA AAACCGAGAC AGAGGAGGCC 
AAACAGAACA ATAATAAAGA AGAAGATGAG AGTGATGTGC CCACTGCTCA GAGACGGAGG 
TTCACGCGGG TGGAAATGGC TCGAGTGCTG ATGGAGAGAA ACCAGTACAA GGAGAGACTG 
ATGGAGCTGC AGGAGGCCGT CCGGTGGACC GAAATGATTC GAGCTTCAAG AGAAAATTCA 
GAAGTCGCAG AAAAGAAGAG GTCAAGCATC AGGCAATTCT TCAGTCGCCT CTTCAGTTCT 
TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC AGAGCTTTAC CGTCCAACGT CAAGTACAAC 
CCACCCAGCA TCATGAAGAA GAGCAACACC TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA 
GCTTTCGACT TCCTCAAAGA AGAGGTGGAC TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG 
AAGCGGGCTC AGTATCAGCA TGTGAAAGCA CACATGCAGA AAGAGGACGG CAGGGTGACC 
GCACATGGCT GGAGTCTGCC GGGAAAATAC AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC 
AAAATAAACC TGCCGGTTCC TGTGTGTCTC AAGCCACTGG AGCAGAAAGA TGCTTCTATG 
AAGCTGCTGT GTGCCACTGG AGTGAATCTC TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT 
GCTGAAGACT CCACAGTAGA GACAGACTCA GCCAAACAGA GGAAAGGCTC TCAGAGCAGC 
CTAGATCAGC TGGAGCACGA TCCTAAGGAG CGGGAGCAGA CGCTGCATGT GGAAATGTCC 
AGTCGCGTCT GGATCTGCAC GAGCACTCAC TCCTCTACTA AGGTGATGGT CATAGATGTG 
ACGCGACCCG ACGACCTCCT GGACAGCTTC TACGTCTGTA ACTCATGCGT GCTGTGTATC 
GCCAGCATAC CAGGAGTGTT GGAGACAGAT TATCCTGCGG GGGAGGAGAT TCCTCAGGAT 
CTGGAGGCGG GACCAGACGC TCTATTGGCC AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG 
TTGAGTGACG TGTCGGCGGT TGGCTGTGGT GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG 
GGCAGCACAG GAGCCCACGA ACAGTCTGCT GAACAGAGTC CCGCCAGAGG AAGTGATCTC 
TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT GCCGCTGAGG AGGCTATGGA GGCCACTGAG 
GAGGACCAGG AGAGTCCGGG ACAGGACAGC AGTCAGCCGG GTATCTACAC GGAGCATGTG 
TTTACAGACC CGCTGGGACC CGAGTCTTCT GGGGCGTCAG CAGGCCATGA TAGCCAGAGA 
GAGTCCGATC GGGACAGGGA TGACACGAGC TCAGCAGGGG GCAAGACGGA TCTGGCAAAA 
GAAGAAACGC AGCGGATGAG CAGTGCACTG CCCACCATGT GGCTGGGGGC TCAGAACGGC 
TGTTTATATG TGCACTCATC TGTTGCTCGA TGGAGGAAGT GTCTTCATGC TGTCAAGCTA 
AAGGATTCCA TCTTGAACAT TGTACATGTG AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC 
GGGACACTGG CCATCTTCCA TAGAGGTCTT GACAGCCAGT GGGATTTAAC CAACTACCAT 
CTTCTGGACC TGGGCCGGCC GCATCACTCA ATCCGCTGCA TGACTGTGGT TCACGATAAA 
GTGTGGTGCG GCTACAGGAA CAAGATCTAC ATCATTCAGC CCAAAGCTAT GAAAATCGAG 
AGGTCTTTTG ATGCCCATCC GCGTAAGGAG AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT 
GACGGGATCT GGGTGTCCAT CCGGCTGGAC TCAACGCTTC GCCTATTCCA TGCTCACACC 
TTCCAGCATC TGCAGGACGT AGACATTGAG CCTTACGTCA GCAAAATGTT AGGAACTGGA 
AAACTGGGCT TCTCTTTTGT GAGAATCACT GCTCTGATGG TTTCTTGTAA TCGGCTGTGG 
GTGGGAACAG GAAATGGTGT CATCATCTCC ATCCCTCTGT CAGACTCTGT AGGTCAGTGT 
GGATATCTAG GTCAGAGGGC AGCTAATAAG ACGGTGGGAG CGATTGCCTC TCGTCCTGGT 
GGAGCAGTGC GTGTATACAG CGAGGACGCT GTGGACGGTG GGGTGACCGG CTCATACGTC 
CCGTACTGCT CCATGGCACA TGCTCAGCTC TGCTTCCATG GCCACAGGGA CGCTGTTAAA 
TTTTTCGCTA CAGTTCCAGG TCAGGTTATT GTGCCTGGAG CTGAGGGTGT GACTGAGAGC 
TCTGAGTCTT CAGATGGAAA GAGCATGTTG ATCATGAGTG GAGGAGAGGG ATACATCGAC 
TTCAGAGTGG GTGATGAGGA TGGAGGATTG GGACGAGATG AAGATGAAGA ATCAGCTGAA 
CCCACACTGA AACTCCAGTC GGCTCCTGCC AAACCTGAGC GCAGTTATGT TATAGTCTGG 
CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209863.1
CDS	579..4499
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKLEAEDPPKKREGKDVLEAWDSLANDCKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354374.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354374.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGCTGGA GGCCGAGGAC CCTCCTAAGA AAAGGGAGGG TAAGGATGTT 
CTGGAAGCTT GGGATTCGCT GGCAAACGAC TGCAAGGATG AGCTGTCTGA CCTCAGCCAG 
GGCGGCTCCA AGTCTGTGAC CCCCATGTCC ACCACAGCCT CTGACCTGGC CCTGGATTCA 
CACAGAGAAG ACGGAGACGC TTTCTGGAAG AACACAGAGG TGCAGGCTGT TCCCGGGACC 
AGAAGCGTCT CTGCAGAAGT GCGAGTAAAT AGTTTGACTG ATAAAGAGGA GAAGTCAGAT 
GTTCAGGCCA TTATTGAATC CACACCGGAG CTGGACATGG ACCTTGGATA TAAAGGCCCA 
AGTACGCCGA CCCGAGGGAT TGAGAACATG GCGTTTGACC GCAACACAGA ATCCCTGTTT 
GAAGAAGTGT CATCGGCGGG CACGGGACTG ATCGGAGATG TGGATGAAGG GGCCGATCTG 
TTGGGCATGA GTCGTGAGGT GGAGAATCTC ATTCAGGAAA ATGTGCAGTT GCTTGAGACA 
AAAAATGCAT TGAATGTGGT AAAGAATGAT CTGATCGCTC GCGTGGACGA GCTCTCCTGC 
GAGAAGGAGG TCCTTCAGGG GGAACTGGAT GCGGTTTTGC AGGCCAAAGC CAAACTAGAG 
GAGAAATACA AGGAGCTGGA GGAGGAGCTC AAAAAAATAA AAACCGAGAC AGAGGAGGCC 
AAACAGAACA ATAATAAAGA AGAAGATGAG AGTGATGTGC CCACTGCTCA GAGACGGAGG 
TTCACGCGGG TGGAAATGGC TCGAGTGCTG ATGGAGAGAA ACCAGTACAA GGAGAGACTG 
ATGGAGCTGC AGGAGGCCGT CCGGTGGACC GAAATGATTC GAGCTTCAAG AGAAAATTCA 
GAAGTCGCAG AAAAGAAGAG GTCAAGCATC AGGCAATTCT TCAGTCGCCT CTTCAGTTCT 
TCCAGTTCTG CTGCGTCCAG CAGCTCGTCC AGAGCTTTAC CGTCCAACGT CAAGTACAAC 
CCACCCAGCA TCATGAAGAA GAGCAACACC TTCTCCCAGT TCCCCAGAGA AAAGTCCAAA 
GCTTTCGACT TCCTCAAAGA AGAGGTGGAC TCGAGTGGCT CTCTGTCCCG CCGGGAGCAG 
AAGCGGGCTC AGTATCAGCA TGTGAAAGCA CACATGCAGA AAGAGGACGG CAGGGTGACC 
GCACATGGCT GGAGTCTGCC GGGAAAATAC AAGGTTGCCA ATGGTGGCCC TGTAGAGAAC 
AAAATAAACC TGCCGGTTCC TGTGTGTCTC AAGCCACTGG AGCAGAAAGA TGCTTCTATG 
AAGCTGCTGT GTGCCACTGG AGTGAATCTC TCTGGAGGAC GCTTGTGTGA CAGAGGCTCT 
GCTGAAGACT CCACAGTAGA GACAGACTCA GCCAAACAGA GGAAAGGCTC TCAGAGCAGC 
CTAGATCAGC TGGAGCACGA TCCTAAGGAG CGGGAGCAGA CGCTGCATGT GGAAATGTCC 
AGTCGCGTCT GGATCTGCAC GAGCACTCAC TCCTCTACTA AGGTGATGGT CATAGATGTG 
ACGCGACCCG ACGACCTCCT GGACAGCTTC TACGTCTGTA ACTCATGCGT GCTGTGTATC 
GCCAGCATAC CAGGAGTGTT GGAGACAGAT TATCCTGCGG GGGAGGAGAT TCCTCAGGAT 
CTGGAGGCGG GACCAGACGC TCTATTGGCC AGCAGCAGCT CTCAGGGCAG TGAGGGAGTG 
TTGAGTGACG TGTCGGCGGT TGGCTGTGGT GTTGAGGAGT CCAGTTTAGG CTCCCAAGCG 
GGCAGCACAG GAGCCCACGA ACAGTCTGCT GAACAGAGTC CCGCCAGAGG AAGTGATCTC 
TCTAGAGCTC CCAGTGAAGA TGGTCTCCCT GCCGCTGAGG AGGCTATGGA GGCCACTGAG 
GAGGACCAGG AGAGTCCGGG ACAGGACAGC AGTCAGCCGG GTATCTACAC GGAGCATGTG 
TTTACAGACC CGCTGGGACC CGAGTCTTCT GGGGCGTCAG CAGGCCATGA TAGCCAGAGA 
GAGTCCGATC GGGACAGGGA TGACACGAGC TCAGCAGGGG GCAAGACGGA TCTGGCAAAA 
GAAGAAACGC AGCGGATGAG CAGTGCACTG CCCACCATGT GGCTGGGGGC TCAGAACGGC 
TGTTTATATG TGCACTCATC TGTTGCTCGA TGGAGGAAGT GTCTTCATGC TGTCAAGCTA 
AAGGATTCCA TCTTGAACAT TGTACATGTG AAAGGCCGTG TTTTGGTGGC TCTAGCTGAC 
GGGACACTGG CCATCTTCCA TAGAGGTCTT GACAGCCAGT GGGATTTAAC CAACTACCAT 
CTTCTGGACC TGGGCCGGCC GCATCACTCA ATCCGCTGCA TGACTGTGGT TCACGATAAA 
GTGTGGTGCG GCTACAGGAA CAAGATCTAC ATCATTCAGC CCAAAGCTAT GAAAATCGAG 
AGGTCTTTTG ATGCCCATCC GCGTAAGGAG AGTCAGGTGC GTCAGCTGGC CTGGGTGGGT 
GACGGGATCT GGGTGTCCAT CCGGCTGGAC TCAACGCTTC GCCTATTCCA TGCTCACACC 
TTCCAGCATC TGCAGGACGT AGACATTGAG CCTTACGTCA GCAAAATGTT AGGAACTGGA 
AAACTGGGCT TCTCTTTTGT GAGAATCACT GCTCTGATGG TTTCTTGTAA TCGGCTGTGG 
GTGGGAACAG GAAATGGTGT CATCATCTCC ATCCCTCTGT CAGACTCTGT AGGTCAGTGT 
GGATATCTAG GTCAGAGGGC AGCTAATAAG ACGGTGGGAG CGATTGCCTC TCGTCCTGGT 
GGAGCAGTGC GTGTATACAG CGAGGACGCT GTGGACGGTG GGGTGACCGG CTCATACGTC 
CCGTACTGCT CCATGGCACA TGCTCAGCTC TGCTTCCATG GCCACAGGGA CGCTGTTAAA 
TTTTTCGCTA CAGTTCCAGG TCAGGTTATT GTGCCTGGAG CTGAGGGTGT GACTGAGAGC 
TCTGAGTCTT CAGATGGAAA GAGCATGTTG ATCATGAGTG GAGGAGAGGG ATACATCGAC 
TTCAGAGTGG GTGATGAGGA TGGAGGATTG GGACGAGATG AAGATGAAGA ATCAGCTGAA 
CCCACACTGA AACTCCAGTC GGCTCCTGCC AAACCTGAGC GCAGTTATGT TATAGTCTGG 
CAGGTTGCGA GTGACCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spag9a ( XM_017354375.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209864.1 Danio rerio sperm associated antigen 9a (spag9a), transcript variant X8, mRNA.

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CloneID	ODa58062
Clone ID Related Accession (Same CDS sequence)	XM_017354375.1
Accession Version	XM_017354375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGGATGA GCTGTCTGAC CTCAGCCAGG GCGGCTCCAA GTCTGTGACC 
CCCATGTCCA CCACAGCCTC TGACCTGGCC CTGGATTCAC ACAGAGAAGA CGGAGACGCT 
TTCTGGAAGA ACACAGAGGT GCAGGCTGTT CCCGGGACCA GAAGCGTCTC TGCAGAAGTG 
CGAGTAAATA GTTTGACTGA TAAAGAGGAG AAGTCAGATG TTCAGGCCAT TATTGAATCC 
ACACCGGAGC TGGACATGGA CCTTGGATAT AAAGGCCCAA GTACGCCGAC CCGAGGGATT 
GAGAACATGG CGTTTGACCG CAACACAGAA TCCCTGTTTG AAGAAGTGTC ATCGGCGGGC 
ACGGGACTGA TCGGAGATGT GGATGAAGGG GCCGATCTGT TGGTGGAGTA CTCTGTGCAT 
GCGCTTTCAC ACCCGAGTCT GTCCGGCATG AGTCGTGAGG TGGAGAATCT CATTCAGGAA 
AATGTGCAGT TGCTTGAGAC AAAAAATGCA TTGAATGTGG TAAAGAATGA TCTGATCGCT 
CGCGTGGACG AGCTCTCCTG CGAGAAGGAG GTCCTTCAGG GGGAACTGGA TGCGGTTTTG 
CAGGCCAAAG CCAAACTAGA GGAGAAATAC AAGGAGCTGG AGGAGGAGCT CAAAAAAATA 
AAAACCGAGA CAGAGGAGGC CAAACAGAAC AATAATAAAG AAGAAGATGA GAGTGATGTG 
CCCACTGCTC AGAGACGGAG GTTCACGCGG GTGGAAATGG CTCGAGTGCT GATGGAGAGA 
AACCAGTACA AGGAGAGACT GATGGAGCTG CAGGAGGCCG TCCGGTGGAC CGAAATGATT 
CGAGCTTCAA GAGAAAATTC AGAAGTCGCA GAAAAGAAGA GGTCAAGCAT CAGGCAATTC 
TTCAGTCGCC TCTTCAGTTC TTCCAGTTCT GCTGCGTCCA GCAGCTCGTC CAGAGCTTTA 
CCGTCCAACG TCAAGTACAA CCCACCCAGC ATCATGAAGA AGAGCAACAC CTTCTCCCAG 
TTCCCCAGAG AAAAGTCCAA AGCTTTCGAC TTCCTCAAAG AAGAGGTGGA CTCGAGTGGC 
TCTCTGTCCC GCCGGGAGCA GAAGCGGGCT CAGTATCAGC ATGTGAAAGC ACACATGCAG 
AAAGAGGACG GCAGGGTGAC CGCACATGGC TGGAGTCTGC CGGGAAAATA CAAGGTTGCC 
AATGGTGGCC CTGTAGAGAA CAAAATAAAC CTGCCGGTTC CTGTGTGTCT CAAGCCACTG 
GAGCAGAAAG ATGCTTCTAT GAAGCTGCTG TGTGCCACTG GAGTGAATCT CTCTGGAGGA 
CGCTTGTGTG ACAGAGGCTC TGCTGAAGAC TCCACAGTAG AGACAGACTC AGCCAAACAG 
AGGAAAGGCT CTCAGAGCAG CCTAGATCAG CTGGAGCACG ATCCTAAGGA GCGGGAGCAG 
ACGCTGCATG TGGAAATGTC CAGTCGCGTC TGGATCTGCA CGAGCACTCA CTCCTCTACT 
AAGGTGATGG TCATAGATGT GACGCGACCC GACGACCTCC TGGACAGCTT CTACGTCTGT 
AACTCATGCG TGCTGTGTAT CGCCAGCATA CCAGGAGTGT TGGAGACAGA TTATCCTGCG 
GGGGAGGAGA TTCCTCAGGA TCTGGAGGCG GGACCAGACG CTCTATTGGC CAGCAGCAGC 
TCTCAGGGCA GTGAGGGAGT GTTGAGTGAC GTGTCGGCGG TTGGCTGTGG TGTTGAGGAG 
TCCAGTTTAG GCTCCCAAGC GGGCAGCACA GGAGCCCACG AACAGTCTGC TGAACAGAGT 
CCCGCCAGAG GAAGTGATCT CTCTAGAGCT CCCAGTGAAG ATGGTCTCCC TGCCGCTGAG 
GAGGCTATGG AGGCCACTGA GGAGGACCAG GAGAGTCCGG GACAGGACAG CAGTCAGCCG 
GGTATCTACA CGGAGCATGT GTTTACAGAC CCGCTGGGAC CCGAGTCTTC TGGGGCGTCA 
GCAGGCCATG ATAGCCAGAG AGAGTCCGAT CGGGACAGGG ATGACACGAG CTCAGCAGGG 
GGCAAGACGG ATCTGGCAAA AGAAGAAACG CAGCGGATGA GCAGTGCACT GCCCACCATG 
TGGCTGGGGG CTCAGAACGG CTGTTTATAT GTGCACTCAT CTGTTGCTCG ATGGAGGAAG 
TGTCTTCATG CTGTCAAGCT AAAGGATTCC ATCTTGAACA TTGTACATGT GAAAGGCCGT 
GTTTTGGTGG CTCTAGCTGA CGGGACACTG GCCATCTTCC ATAGAGGTCT TGACAGCCAG 
TGGGATTTAA CCAACTACCA TCTTCTGGAC CTGGGCCGGC CGCATCACTC AATCCGCTGC 
ATGACTGTGG TTCACGATAA AGTGTGGTGC GGCTACAGGA ACAAGATCTA CATCATTCAG 
CCCAAAGCTA TGAAAATCGA GAGGTCTTTT GATGCCCATC CGCGTAAGGA GAGTCAGGTG 
CGTCAGCTGG CCTGGGTGGG TGACGGGATC TGGGTGTCCA TCCGGCTGGA CTCAACGCTT 
CGCCTATTCC ATGCTCACAC CTTCCAGCAT CTGCAGGACG TAGACATTGA GCCTTACGTC 
AGCAAAATGT TAGGAACTGG AAAACTGGGC TTCTCTTTTG TGAGAATCAC TGCTCTGATG 
GTTTCTTGTA ATCGGCTGTG GGTGGGAACA GGAAATGGTG TCATCATCTC CATCCCTCTG 
TCAGACTCTG TAGGTCAGTG TGGATATCTA GGTCAGAGGG CAGCTAATAA GACGGTGGGA 
GCGATTGCCT CTCGTCCTGG TGGAGCAGTG CGTGTATACA GCGAGGACGC TGTGGACGGT 
GGGGTGACCG GCTCATACGT CCCGTACTGC TCCATGGCAC ATGCTCAGCT CTGCTTCCAT 
GGCCACAGGG ACGCTGTTAA ATTTTTCGCT ACAGTTCCAG GTCAGGTTAT TGTGCCTGGA 
GCTGAGGGTG TGACTGAGAG CTCTGAGTCT TCAGATGGAA AGAGCATGTT GATCATGAGT 
GGAGGAGAGG GATACATCGA CTTCAGAGTG GGTGATGAGG ATGGAGGATT GGGACGAGAT 
GAAGATGAAG AATCAGCTGA ACCCACACTG AAACTCCAGT CGGCTCCTGC CAAACCTGAG 
CGCAGTTATG TTATAGTCTG GCAGGTTGCG AGTGACCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209864.1
CDS	579..4460
Translation	MELDDGVVYQDDPGTSAVMSERISGLANSIYREFERLIRKYDEDVVKELMPLVVAVLENLDTVFAENQEHEVELELLKEDNEQLVTQYEREKALRKHTEERFIEFEDSQEQEKKDLQNHLESLGSHSRQLELKIKNYSDQISRLEEREAELKREYNSLHLRHTEMIHNYMEHVERIRMQQTVGEASDTGTMGRVRKERPLSLGIFPMSAGGSSLTPDVQGRAETPGMEGWRFNNLSHQRSNASLKDELSDLSQGGSKSVTPMSTTASDLALDSHREDGDAFWKNTEVQAVPGTRSVSAEVRVNSLTDKEEKSDVQAIIESTPELDMDLGYKGPSTPTRGIENMAFDRNTESLFEEVSSAGTGLIGDVDEGADLLVEYSVHALSHPSLSGMSREVENLIQENVQLLETKNALNVVKNDLIARVDELSCEKEVLQGELDAVLQAKAKLEEKYKELEEELKKIKTETEEAKQNNNKEEDESDVPTAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENSEVAEKKRSSIRQFFSRLFSSSSSAASSSSSRALPSNVKYNPPSIMKKSNTFSQFPREKSKAFDFLKEEVDSSGSLSRREQKRAQYQHVKAHMQKEDGRVTAHGWSLPGKYKVANGGPVENKINLPVPVCLKPLEQKDASMKLLCATGVNLSGGRLCDRGSAEDSTVETDSAKQRKGSQSSLDQLEHDPKEREQTLHVEMSSRVWICTSTHSSTKVMVIDVTRPDDLLDSFYVCNSCVLCIASIPGVLETDYPAGEEIPQDLEAGPDALLASSSSQGSEGVLSDVSAVGCGVEESSLGSQAGSTGAHEQSAEQSPARGSDLSRAPSEDGLPAAEEAMEATEEDQESPGQDSSQPGIYTEHVFTDPLGPESSGASAGHDSQRESDRDRDDTSSAGGKTDLAKEETQRMSSALPTMWLGAQNGCLYVHSSVARWRKCLHAVKLKDSILNIVHVKGRVLVALADGTLAIFHRGLDSQWDLTNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYIIQPKAMKIERSFDAHPRKESQVRQLAWVGDGIWVSIRLDSTLRLFHAHTFQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLSDSVGQCGYLGQRAANKTVGAIASRPGGAVRVYSEDAVDGGVTGSYVPYCSMAHAQLCFHGHRDAVKFFATVPGQVIVPGAEGVTESSESSDGKSMLIMSGGEGYIDFRVGDEDGGLGRDEDEESAEPTLKLQSAPAKPERSYVIVWQVASDR

Target ORF information:

RefSeq Version	XM_017354375.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sperm associated antigen 9a (spag9a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354375.1

ORF Insert Sequence:

ATGGAGCTGG ATGACGGAGT TGTGTACCAG GACGACCCGG GGACATCGGC GGTGATGTCG 
GAGCGGATCT CGGGCCTGGC CAACTCCATC TACCGCGAGT TCGAGAGACT CATCCGCAAA 
TACGACGAGG ACGTGGTGAA GGAGCTGATG CCGCTGGTGG TGGCGGTGCT GGAGAACCTG 
GACACGGTGT TCGCCGAAAA CCAGGAGCAC GAAGTGGAGC TCGAGCTGCT GAAGGAGGAC 
AACGAGCAGC TCGTCACTCA GTACGAGCGC GAGAAAGCGC TGAGGAAACA CACGGAGGAG 
AGGTTCATTG AATTTGAAGA TTCCCAAGAG CAAGAGAAGA AGGACTTGCA GAACCATCTG 
GAGAGCCTGG GATCACATTC CCGTCAGCTG GAGCTGAAGA TCAAAAACTA TTCTGACCAG 
ATAAGTCGAT TAGAGGAGCG AGAGGCTGAA CTTAAGCGAG AGTATAATTC CCTTCACCTG 
CGACACACAG AGATGATCCA CAATTACATG GAGCATGTGG AACGTATTAG GATGCAGCAG 
ACGGTAGGAG AGGCTTCTGA CACAGGAACA ATGGGCAGAG TTCGGAAAGA GCGGCCTCTG 
TCTCTGGGGA TCTTCCCCAT GTCTGCCGGA GGCTCTTCAC TGACGCCTGA TGTCCAGGGC 
CGAGCAGAGA CTCCTGGGAT GGAGGGCTGG AGATTCAACA ATCTCAGCCA TCAGCGCTCT 
AATGCCAGCC TCAAGGATGA GCTGTCTGAC CTCAGCCAGG GCGGCTCCAA GTCTGTGACC 
CCCATGTCCA CCACAGCCTC TGACCTGGCC CTGGATTCAC ACAGAGAAGA CGGAGACGCT 
TTCTGGAAGA ACACAGAGGT GCAGGCTGTT CCCGGGACCA GAAGCGTCTC TGCAGAAGTG 
CGAGTAAATA GTTTGACTGA TAAAGAGGAG AAGTCAGATG TTCAGGCCAT TATTGAATCC 
ACACCGGAGC TGGACATGGA CCTTGGATAT AAAGGCCCAA GTACGCCGAC CCGAGGGATT 
GAGAACATGG CGTTTGACCG CAACACAGAA TCCCTGTTTG AAGAAGTGTC ATCGGCGGGC 
ACGGGACTGA TCGGAGATGT GGATGAAGGG GCCGATCTGT TGGTGGAGTA CTCTGTGCAT 
GCGCTTTCAC ACCCGAGTCT GTCCGGCATG AGTCGTGAGG TGGAGAATCT CATTCAGGAA 
AATGTGCAGT TGCTTGAGAC AAAAAATGCA TTGAATGTGG TAAAGAATGA TCTGATCGCT 
CGCGTGGACG AGCTCTCCTG CGAGAAGGAG GTCCTTCAGG GGGAACTGGA TGCGGTTTTG 
CAGGCCAAAG CCAAACTAGA GGAGAAATAC AAGGAGCTGG AGGAGGAGCT CAAAAAAATA 
AAAACCGAGA CAGAGGAGGC CAAACAGAAC AATAATAAAG AAGAAGATGA GAGTGATGTG 
CCCACTGCTC AGAGACGGAG GTTCACGCGG GTGGAAATGG CTCGAGTGCT GATGGAGAGA 
AACCAGTACA AGGAGAGACT GATGGAGCTG CAGGAGGCCG TCCGGTGGAC CGAAATGATT 
CGAGCTTCAA GAGAAAATTC AGAAGTCGCA GAAAAGAAGA GGTCAAGCAT CAGGCAATTC 
TTCAGTCGCC TCTTCAGTTC TTCCAGTTCT GCTGCGTCCA GCAGCTCGTC CAGAGCTTTA 
CCGTCCAACG TCAAGTACAA CCCACCCAGC ATCATGAAGA AGAGCAACAC CTTCTCCCAG 
TTCCCCAGAG AAAAGTCCAA AGCTTTCGAC TTCCTCAAAG AAGAGGTGGA CTCGAGTGGC 
TCTCTGTCCC GCCGGGAGCA GAAGCGGGCT CAGTATCAGC ATGTGAAAGC ACACATGCAG 
AAAGAGGACG GCAGGGTGAC CGCACATGGC TGGAGTCTGC CGGGAAAATA CAAGGTTGCC 
AATGGTGGCC CTGTAGAGAA CAAAATAAAC CTGCCGGTTC CTGTGTGTCT CAAGCCACTG 
GAGCAGAAAG ATGCTTCTAT GAAGCTGCTG TGTGCCACTG GAGTGAATCT CTCTGGAGGA 
CGCTTGTGTG ACAGAGGCTC TGCTGAAGAC TCCACAGTAG AGACAGACTC AGCCAAACAG 
AGGAAAGGCT CTCAGAGCAG CCTAGATCAG CTGGAGCACG ATCCTAAGGA GCGGGAGCAG 
ACGCTGCATG TGGAAATGTC CAGTCGCGTC TGGATCTGCA CGAGCACTCA CTCCTCTACT 
AAGGTGATGG TCATAGATGT GACGCGACCC GACGACCTCC TGGACAGCTT CTACGTCTGT 
AACTCATGCG TGCTGTGTAT CGCCAGCATA CCAGGAGTGT TGGAGACAGA TTATCCTGCG 
GGGGAGGAGA TTCCTCAGGA TCTGGAGGCG GGACCAGACG CTCTATTGGC CAGCAGCAGC 
TCTCAGGGCA GTGAGGGAGT GTTGAGTGAC GTGTCGGCGG TTGGCTGTGG TGTTGAGGAG 
TCCAGTTTAG GCTCCCAAGC GGGCAGCACA GGAGCCCACG AACAGTCTGC TGAACAGAGT 
CCCGCCAGAG GAAGTGATCT CTCTAGAGCT CCCAGTGAAG ATGGTCTCCC TGCCGCTGAG 
GAGGCTATGG AGGCCACTGA GGAGGACCAG GAGAGTCCGG GACAGGACAG CAGTCAGCCG 
GGTATCTACA CGGAGCATGT GTTTACAGAC CCGCTGGGAC CCGAGTCTTC TGGGGCGTCA 
GCAGGCCATG ATAGCCAGAG AGAGTCCGAT CGGGACAGGG ATGACACGAG CTCAGCAGGG 
GGCAAGACGG ATCTGGCAAA AGAAGAAACG CAGCGGATGA GCAGTGCACT GCCCACCATG 
TGGCTGGGGG CTCAGAACGG CTGTTTATAT GTGCACTCAT CTGTTGCTCG ATGGAGGAAG 
TGTCTTCATG CTGTCAAGCT AAAGGATTCC ATCTTGAACA TTGTACATGT GAAAGGCCGT 
GTTTTGGTGG CTCTAGCTGA CGGGACACTG GCCATCTTCC ATAGAGGTCT TGACAGCCAG 
TGGGATTTAA CCAACTACCA TCTTCTGGAC CTGGGCCGGC CGCATCACTC AATCCGCTGC 
ATGACTGTGG TTCACGATAA AGTGTGGTGC GGCTACAGGA ACAAGATCTA CATCATTCAG 
CCCAAAGCTA TGAAAATCGA GAGGTCTTTT GATGCCCATC CGCGTAAGGA GAGTCAGGTG 
CGTCAGCTGG CCTGGGTGGG TGACGGGATC TGGGTGTCCA TCCGGCTGGA CTCAACGCTT 
CGCCTATTCC ATGCTCACAC CTTCCAGCAT CTGCAGGACG TAGACATTGA GCCTTACGTC 
AGCAAAATGT TAGGAACTGG AAAACTGGGC TTCTCTTTTG TGAGAATCAC TGCTCTGATG 
GTTTCTTGTA ATCGGCTGTG GGTGGGAACA GGAAATGGTG TCATCATCTC CATCCCTCTG 
TCAGACTCTG TAGGTCAGTG TGGATATCTA GGTCAGAGGG CAGCTAATAA GACGGTGGGA 
GCGATTGCCT CTCGTCCTGG TGGAGCAGTG CGTGTATACA GCGAGGACGC TGTGGACGGT 
GGGGTGACCG GCTCATACGT CCCGTACTGC TCCATGGCAC ATGCTCAGCT CTGCTTCCAT 
GGCCACAGGG ACGCTGTTAA ATTTTTCGCT ACAGTTCCAG GTCAGGTTAT TGTGCCTGGA 
GCTGAGGGTG TGACTGAGAG CTCTGAGTCT TCAGATGGAA AGAGCATGTT GATCATGAGT 
GGAGGAGAGG GATACATCGA CTTCAGAGTG GGTGATGAGG ATGGAGGATT GGGACGAGAT 
GAAGATGAAG AATCAGCTGA ACCCACACTG AAACTCCAGT CGGCTCCTGC CAAACCTGAG 
CGCAGTTATG TTATAGTCTG GCAGGTTGCG AGTGACCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	spag9a
Entrez Gene ID	558833
Full Name	sperm associated antigen 9a
Synonyms	zgc:165467
General protein information	Preferred Names sperm associated antigen 9a Names C-Jun-amino-terminal kinase-interacting protein 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

spag9a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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