thsd7aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	thsd7aa
Entrez Gene ID	557991
Full Name	thrombospondin, type I, domain containing 7Aa
Synonyms	si:dkey-12h3.1,thsd7a
General protein information	Preferred Names thrombospondin, type I, domain containing 7Aa Names thrombospondin type-1 domain-containing protein 7A thrombospondin, type I, domain containing 7A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159680.3	XP_005159737.1	thrombospondin type-1 domain-containing protein 7A isoform X2
NM_001122760.1	NP_001116232.1	thrombospondin type-1 domain-containing protein 7A precursor
XM_009294351.2	XP_009292626.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_009294351.3	XP_009292626.1	thrombospondin type-1 domain-containing protein 7A isoform X1
XM_005159680.4	XP_005159737.1	thrombospondin type-1 domain-containing protein 7A isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	THSD7A	NP_056019.1
Canis lupus familiaris (dog)	THSD7A	XP_003431913.2
Bos taurus (cattle)	THSD7A	NP_001193672.1
Danio rerio (zebrafish)	thsd7a	NP_001116232.1
Mus musculus (house mouse)	Thsd7a	NP_001158277.1
Pan troglodytes (chimpanzee)	THSD7A	XP_527669.2
Rattus norvegicus (Norway rat)	Thsd7a	NP_001178899.1
Gallus gallus (chicken)	THSD7A	XP_418686.3
Xenopus tropicalis (tropical clawed frog)	thsd7a	XP_002939058.2

Gene Ontology
Bibliography

Related articles in PubMed

A Heterologous Model of Thrombospondin Type 1 Domain-Containing 7A-Associated Membranous Nephropathy.
Tomas NM, Meyer-Schwesinger C, von Spiegel H, Kotb AM, Zahner G, Hoxha E, Helmchen U, Endlich N, Koch-Nolte F, Stahl RAK
Journal of the American Society of Nephrology : JASN28(11)3262-3277(2017 Nov)

Motor neuron-derived Thsd7a is essential for zebrafish vascular development via the Notch-dll4 signaling pathway.
Liu LY, Lin MH, Lai ZY, Jiang JP, Huang YC, Jao LE, Chuang YJ
Journal of biomedical science23(1)59(2016 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish Thsd7a is a neural protein required for angiogenic patterning during development.
Wang CH, Chen IH, Kuo MW, Su PT, Lai ZY, Wang CH, Huang WC, Hoffman J, Kuo CJ, You MS, Chuang YJ
Developmental dynamics : an official publication of the American Association of Anatomists240(6)1412-21(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An important role of Thsd7a in the neurovascular interaction during zebrafish development.
Title: Zebrafish Thsd7a is a neural protein required for angiogenic patterning during development.

The following thsd7aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thsd7aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34559	NM_001122760.1 Latest version!	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa24344	XM_005159680.4 Latest version!	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42716	XM_009294351.3 Latest version!	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa42716	XM_009294351.2	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa24344	XM_005159680.3	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thsd7aa ( NM_001122760.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116232.1 Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), mRNA.

Price & Availability↑

CloneID	ODa34559
Clone ID Related Accession (Same CDS sequence)	NM_001122760.1
Accession Version	NM_001122760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR735126.7, CR627498.9 and CR381594.6. On Mar 19, 2008 this sequence version replaced XM_681154.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505390, SAMEA3505391 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGTG TATGAAGTGG TTCCGTGTCT GAGTGACTGC 
AGTCAGTATG TCTGGGTTGC TGAGCCATGG AGCGTATGGA AAGTCAGCAA TGTCGATCTG 
AAGGAGAACT GTGGAGAAGG TGTACAGACT AGGAAAGTCA GGTGCATGCA GAACACTGTT 
GATGGACCAT CAGACCCTGT TGAAGACTAT CTGTGTGACC CAGAGGAGAT GCCGCTTGGA 
GCCAGAGAAA GCAAACTGCC CTGTCCTGAG GACTGCGTTC TGACCGACTG GGGCTCCTGG 
AGTCGATGCC CGCTGCCGTG TAATGTGAAT AGTACAAGGC AGAGGAGCGC CAGTCCTATC 
CGTCAGCCCA GTGAGAGGAA ACAGTGTCCT TCCACCACAG AGAAAGAAAT ATGCACACTC 
AACTCCAACT GCTTCCATTA CTCCTATAAT ATCACAGACT GGAGCACGTG TCAACTGAGT 
GAGCGAGCCG TGTGCGGGGT TGGATTCAAG ACGCGAATGC TGGACTGTGT TCGCAGCGAC 
AGCAAATCTG TGGACCTCAA GTTTTGTGAA GAGCTCGGTC TGGAGAAGAA ATGGCAGATG 
AACGCGTCTT GTGTGGTCGA GTGTCCTGTG AACTGCCAGC TGTCCGACTG GTCTTCTTGG 
TCTGAATGCT CTCACACTTG TGGTCTTGCA GGGAAGCTGT GGAGAAGGAG GACTGTGATA 
CAGGCGTCTC AGGGAGATGG ACGACCGTGT TCCTCTCAGC TGGAGCAGTG GAAACCCTGT 
CCTGTCAAAC CCTGCTTCAG CTGGAGGTAC AGTGTCTGGT CCCCATGCAA ATCTGAGGGT 
GCACGGTGTG GTGAAGGCCT TCGCTTCAGA AACGTGTCCT GCTTCGTGTC CGATGGCTCA 
GGGAAGGATG CGGGCAGTAT GGTGGATGAA GAACTGTGTG GAGATCTGGA GCAGACAGTG 
GATGGAGACA AACAGATCAT CCTGCAGGAG TCCTGCACTG TGCCCTGCCC AGGTGAGTGT 
TACCTGACAG ACTGGACGAT GTGGAGCCCT TGTCAACTCA GCTGTATCGG CGGGGATGAT 
CTGGGCTTCG GGTCAGTGCA GGTTCGTTCT CGCGCTGTTC TCGCTCAGGA ACCCGAAAAC 
CTTCTTCAGT GTCCAGAACA AGAATGGGAG GCCAGACCCT GTACAGAGGG TCAGTGTTAT 
GATTACAAAT GGATGACCGG CGCATGGAGG GGCTCGTCTC GACAAGTTTG GTGCCAGCGC 
TCGGATGGAT TAAACGTCAC AGGTGGATGC CAATCGACGA CCGAGCCGGT GTCAGACAGA 
TCCTGTGACC CAGCCTGCGA CAAGCCACGC TCCATCTGCA CTGAGGCCGG GATCTGCGGC 
TGTGAGGAAG GCTACACTGA GGTCATGACA TCTGATGGAG TTTTAGATCA GTGTACGGTC 
ATCCCAGTGC TGGAGATTCC CACCGCAGGA GACAGTAAAG CTGACGTCAA GACCATAAGA 
GCCCTGAACC CCACGGAGCC CACCGCAAAC ATGCCCGGAC GCGCCGGACG CACGTGGTTC 
CTGCAACCTT TTGGTCCCGA TGGGAAACTG AAGACCTGGG TTTATGGTGT GGCAGCTGGA 
GCTTTTGTCC TGCTAGTGTT CATTGTCTCT ATGACTTACT TAGCCTGCAA AAAGCCAAAG 
AAGCCCCAGA GACGACAGAT GAACAACAGA CTCAAGCCTC TGACTCTGGC TTATGACGGC 
GATGCTGACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116232.1
CDS	370..5424
Translation	MGLASRAPGKGGTSAGALASLFRVALLFFGLSDVQTQTVANTRPTYIWQTGPWGRCMGSECGPGGSQSRAVWCAHSEGWTTLHTNCQQSERPSNQQSCFRVCDWHKELYDWQLGAWNQCVPVSMRNAGVPRPAVCTRGEEGIQTREVGCVHKSDGVPAEDAICEYFEPKPRLEQACLIPCPRDCVVSEFSPWTSCSKSCGMGLQNRLRSVLAPPLFGGSACPNLTEFRTCQPGKCEGVESLHSLRVGPWGQCMASPIRQARDTGEARVPKAERKAKRDRQARQERQGKRRKNKEKKELRESKGERVRERVKEKKRMRDPETRELIKKKRTRNRQNRQGLKFWDLQVGYQTREVTCVHRSGSTASISQCTQQTLPVTYQACVISKDCEVSEWSDWSVCSKECYDLNGRKGQRTRTRQVQQFPVGGGAECPELEESEPCSPQGEGIPPCVVYNWRSTEWSDCRVDVLLSQQDRRRSNQTGLCGGGVQTREVYCVQAPSETSSNLGSLKSKDALRPVNSDLCLGVPHNTTQLCHIPCPVECEVSAWSAWGPCTFENCQDQSTKKGFKLRKRKIMNEPTGGTGNCPHLTEAIPCEEPSCYDWLLVKLEECVPDNDKVCGPGTQNPQVQCINSDGEFVDRQLCRDAILPMPVLCEVSCPKDCVLSPWTSWSLCSNTCSGKNSEGKQTRARSILAYNAGDGGVQCPNSSALQEVRSCNDHPCTVYHWQTGPWGQCIEDTSVPSANSSISRAVPGTAVNDAFCSVGMQTRKVICVRVNVGQVPPKKCPESLRPETVRPCLLPCKRDCVVTPYSDWTPCPSICQTGGSVKKKQSRKRIIIQLPANGGQDCPEVLFQEKDCDASSVCPGYRWKTHKWRRCQLVPWSIRQDSPGAQETCGPGLQARAVSCKKLDSGPADVAACLKFAGPMPQLTQSCQLPCQDDCQLTAWSKFSTCAADCVGVRTRKRTLVGKSKKREQCKNTQMYPLSETQYCPCNKYNAQPVGNWSDCILPEGRVEGLLGMKVQGDIKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQVYEVVPCLSDCSQYVWVAEPWSVWKVSNVDLKENCGEGVQTRKVRCMQNTVDGPSDPVEDYLCDPEEMPLGARESKLPCPEDCVLTDWGSWSRCPLPCNVNSTRQRSASPIRQPSERKQCPSTTEKEICTLNSNCFHYSYNITDWSTCQLSERAVCGVGFKTRMLDCVRSDSKSVDLKFCEELGLEKKWQMNASCVVECPVNCQLSDWSSWSECSHTCGLAGKLWRRRTVIQASQGDGRPCSSQLEQWKPCPVKPCFSWRYSVWSPCKSEGARCGEGLRFRNVSCFVSDGSGKDAGSMVDEELCGDLEQTVDGDKQIILQESCTVPCPGECYLTDWTMWSPCQLSCIGGDDLGFGSVQVRSRAVLAQEPENLLQCPEQEWEARPCTEGQCYDYKWMTGAWRGSSRQVWCQRSDGLNVTGGCQSTTEPVSDRSCDPACDKPRSICTEAGICGCEEGYTEVMTSDGVLDQCTVIPVLEIPTAGDSKADVKTIRALNPTEPTANMPGRAGRTWFLQPFGPDGKLKTWVYGVAAGAFVLLVFIVSMTYLACKKPKKPQRRQMNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	NM_001122760.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122760.1

ORF Insert Sequence:

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGTG TATGAAGTGG TTCCGTGTCT GAGTGACTGC 
AGTCAGTATG TCTGGGTTGC TGAGCCATGG AGCGTATGGA AAGTCAGCAA TGTCGATCTG 
AAGGAGAACT GTGGAGAAGG TGTACAGACT AGGAAAGTCA GGTGCATGCA GAACACTGTT 
GATGGACCAT CAGACCCTGT TGAAGACTAT CTGTGTGACC CAGAGGAGAT GCCGCTTGGA 
GCCAGAGAAA GCAAACTGCC CTGTCCTGAG GACTGCGTTC TGACCGACTG GGGCTCCTGG 
AGTCGATGCC CGCTGCCGTG TAATGTGAAT AGTACAAGGC AGAGGAGCGC CAGTCCTATC 
CGTCAGCCCA GTGAGAGGAA ACAGTGTCCT TCCACCACAG AGAAAGAAAT ATGCACACTC 
AACTCCAACT GCTTCCATTA CTCCTATAAT ATCACAGACT GGAGCACGTG TCAACTGAGT 
GAGCGAGCCG TGTGCGGGGT TGGATTCAAG ACGCGAATGC TGGACTGTGT TCGCAGCGAC 
AGCAAATCTG TGGACCTCAA GTTTTGTGAA GAGCTCGGTC TGGAGAAGAA ATGGCAGATG 
AACGCGTCTT GTGTGGTCGA GTGTCCTGTG AACTGCCAGC TGTCCGACTG GTCTTCTTGG 
TCTGAATGCT CTCACACTTG TGGTCTTGCA GGGAAGCTGT GGAGAAGGAG GACTGTGATA 
CAGGCGTCTC AGGGAGATGG ACGACCGTGT TCCTCTCAGC TGGAGCAGTG GAAACCCTGT 
CCTGTCAAAC CCTGCTTCAG CTGGAGGTAC AGTGTCTGGT CCCCATGCAA ATCTGAGGGT 
GCACGGTGTG GTGAAGGCCT TCGCTTCAGA AACGTGTCCT GCTTCGTGTC CGATGGCTCA 
GGGAAGGATG CGGGCAGTAT GGTGGATGAA GAACTGTGTG GAGATCTGGA GCAGACAGTG 
GATGGAGACA AACAGATCAT CCTGCAGGAG TCCTGCACTG TGCCCTGCCC AGGTGAGTGT 
TACCTGACAG ACTGGACGAT GTGGAGCCCT TGTCAACTCA GCTGTATCGG CGGGGATGAT 
CTGGGCTTCG GGTCAGTGCA GGTTCGTTCT CGCGCTGTTC TCGCTCAGGA ACCCGAAAAC 
CTTCTTCAGT GTCCAGAACA AGAATGGGAG GCCAGACCCT GTACAGAGGG TCAGTGTTAT 
GATTACAAAT GGATGACCGG CGCATGGAGG GGCTCGTCTC GACAAGTTTG GTGCCAGCGC 
TCGGATGGAT TAAACGTCAC AGGTGGATGC CAATCGACGA CCGAGCCGGT GTCAGACAGA 
TCCTGTGACC CAGCCTGCGA CAAGCCACGC TCCATCTGCA CTGAGGCCGG GATCTGCGGC 
TGTGAGGAAG GCTACACTGA GGTCATGACA TCTGATGGAG TTTTAGATCA GTGTACGGTC 
ATCCCAGTGC TGGAGATTCC CACCGCAGGA GACAGTAAAG CTGACGTCAA GACCATAAGA 
GCCCTGAACC CCACGGAGCC CACCGCAAAC ATGCCCGGAC GCGCCGGACG CACGTGGTTC 
CTGCAACCTT TTGGTCCCGA TGGGAAACTG AAGACCTGGG TTTATGGTGT GGCAGCTGGA 
GCTTTTGTCC TGCTAGTGTT CATTGTCTCT ATGACTTACT TAGCCTGCAA AAAGCCAAAG 
AAGCCCCAGA GACGACAGAT GAACAACAGA CTCAAGCCTC TGACTCTGGC TTATGACGGC 
GATGCTGACA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7aa ( XM_005159680.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159737.1 Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X2, mRNA.

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CloneID	ODa24344
Clone ID Related Accession (Same CDS sequence)	XM_005159680.3 , XM_005159680.4
Accession Version	XM_005159680.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGCAAAAAG 
CCAAAGAAGC CCCAGAGACG ACAGATGAAC AACAGACTCA AGCCTCTGAC TCTGGCTTAT 
GACGGCGATG CTGACATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159737.1
CDS	366..5426
Translation	MGLASRAPGKGGTSAGALASLFRVALLFFGLSDVQTQTVANTRPTYIWQTGPWGRCMGSECGPGGSQSRAVWCAHSEGWTTLHTNCQQSERPSNQQSCFRVCDWHKELYDWQLGAWNQCVPVSMRNAGVPRPAVCTRGEEGIQTREVGCVHKSDGVPAEDAICEYFEPKPRLEQACLIPCPRDCVVSEFSPWTSCSKSCGMGLQNRLRSVLAPPLFGGSACPNLTEFRTCQPGKCEGVESLHSLRVGPWGQCMASPIRQARDTGEARVPKAERKAKRDRQARQERQGKRRKNKEKKELRESKGERVRERVKEKKRMRDPETRELIKKKRTRNRQNRQGLKFWDLQVGYQTREVTCVHRSGSTASISQCTQQTLPVTYQACVISKDCEVSEWSDWSVCSKECYDLNGRKGQRTRTRQVQQFPVGGGAECPELEESEPCSPQGEGIPPCVVYNWRSTEWSDCRVDVLLSQQDRRRSNQTGLCGGGVQTREVYCVQAPSETSSNLGSLKSKDALRPVNSDLCLGVPHNTTQLCHIPCPVECEVSAWSAWGPCTFENCQDQSTKKGFKLRKRKIMNEPTGGTGNCPHLTEAIPCEEPSCYDWLLVKLEECVPDNDKVCGPGTQNPQVQCINSDGEFVDRQLCRDAILPMPVLCEVSCPKDCVLSPWTSWSLCSNTCSGKNSEGKQTRARSILAYNAGDGGVQCPNSSALQEVRSCNDHPCTVYHWQTGPWGQCIEDTSVPSANSSISRAVPGTAVNDAFCSVGMQTRKVICVRVNVGQVPPKKCPESLRPETVRPCLLPCKRDCVVTPYSDWTPCPSICQTGGSVKKKQSRKRIIIQLPANGGQDCPEVLFQEKDCDASSVCPGYRWKTHKWRRCQLVPWSIRQDSPGAQETCGPGLQARAVSCKKLDSGPADVAACLKFAGPMPQLTQSCQLPCQDDCQLTAWSKFSTCAADCVGVRTRKRTLVGKSKKREQCKNTQMYPLSETQYCPCNKYNAQPVGNWSDCILPEGRVEGLLGMKVQGDIKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCLSDCSQYVWVAEPWSVWKVSNVDLKENCGEGVQTRKVRCMQNTVDGPSDPVEDYLCDPEEMPLGARESKLPCPEDCVLTDWGSWSRCPLPCNVNSTRQRSASPIRQPSERKQCPSTTEKEICTLNSNCFHYSYNITDWSTCQLSERAVCGVGFKTRMLDCVRSDSKSVDLKFCEELGLEKKWQMNASCVVECPVNCQLSDWSSWSECSHTCGLAGKLWRRRTVIQASQGDGRPCSSQLEQWKPCPVKPCFSWRYSVWSPCKSEGARCGEGLRFRNVSCFVSDGSGKDAGSMVDEELCGDLEQTVDGDKQIILQESCTVPCPGECYLTDWTMWSPCQLSCIGGDDLGFGSVQVRSRAVLAQEPENLLQCPEQEWEARPCTEGQCYDYKWMTGAWRGSSRQVWCQRSDGLNVTGGCQSTTEPVSDRSCDPACDKPRSICTEAGICGCEEGYTEVMTSDGVLDQCTVIPVLEIPTAGDSKADVKTIRALNPTEPTANMPGRAGRTWFLQPFGPDGKLKTWVYGVAAGAFVLLVFIVSMTYLACKKPKKPQRRQMNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_005159680.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159680.3

ORF Insert Sequence:

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGCAAAAAG 
CCAAAGAAGC CCCAGAGACG ACAGATGAAC AACAGACTCA AGCCTCTGAC TCTGGCTTAT 
GACGGCGATG CTGACATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7aa ( XM_005159680.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159737.1 Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24344
Clone ID Related Accession (Same CDS sequence)	XM_005159680.3 , XM_005159680.4
Accession Version	XM_005159680.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159680.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGCAAAAAG 
CCAAAGAAGC CCCAGAGACG ACAGATGAAC AACAGACTCA AGCCTCTGAC TCTGGCTTAT 
GACGGCGATG CTGACATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159737.1
CDS	364..5424
Translation	MGLASRAPGKGGTSAGALASLFRVALLFFGLSDVQTQTVANTRPTYIWQTGPWGRCMGSECGPGGSQSRAVWCAHSEGWTTLHTNCQQSERPSNQQSCFRVCDWHKELYDWQLGAWNQCVPVSMRNAGVPRPAVCTRGEEGIQTREVGCVHKSDGVPAEDAICEYFEPKPRLEQACLIPCPRDCVVSEFSPWTSCSKSCGMGLQNRLRSVLAPPLFGGSACPNLTEFRTCQPGKCEGVESLHSLRVGPWGQCMASPIRQARDTGEARVPKAERKAKRDRQARQERQGKRRKNKEKKELRESKGERVRERVKEKKRMRDPETRELIKKKRTRNRQNRQGLKFWDLQVGYQTREVTCVHRSGSTASISQCTQQTLPVTYQACVISKDCEVSEWSDWSVCSKECYDLNGRKGQRTRTRQVQQFPVGGGAECPELEESEPCSPQGEGIPPCVVYNWRSTEWSDCRVDVLLSQQDRRRSNQTGLCGGGVQTREVYCVQAPSETSSNLGSLKSKDALRPVNSDLCLGVPHNTTQLCHIPCPVECEVSAWSAWGPCTFENCQDQSTKKGFKLRKRKIMNEPTGGTGNCPHLTEAIPCEEPSCYDWLLVKLEECVPDNDKVCGPGTQNPQVQCINSDGEFVDRQLCRDAILPMPVLCEVSCPKDCVLSPWTSWSLCSNTCSGKNSEGKQTRARSILAYNAGDGGVQCPNSSALQEVRSCNDHPCTVYHWQTGPWGQCIEDTSVPSANSSISRAVPGTAVNDAFCSVGMQTRKVICVRVNVGQVPPKKCPESLRPETVRPCLLPCKRDCVVTPYSDWTPCPSICQTGGSVKKKQSRKRIIIQLPANGGQDCPEVLFQEKDCDASSVCPGYRWKTHKWRRCQLVPWSIRQDSPGAQETCGPGLQARAVSCKKLDSGPADVAACLKFAGPMPQLTQSCQLPCQDDCQLTAWSKFSTCAADCVGVRTRKRTLVGKSKKREQCKNTQMYPLSETQYCPCNKYNAQPVGNWSDCILPEGRVEGLLGMKVQGDIKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCLSDCSQYVWVAEPWSVWKVSNVDLKENCGEGVQTRKVRCMQNTVDGPSDPVEDYLCDPEEMPLGARESKLPCPEDCVLTDWGSWSRCPLPCNVNSTRQRSASPIRQPSERKQCPSTTEKEICTLNSNCFHYSYNITDWSTCQLSERAVCGVGFKTRMLDCVRSDSKSVDLKFCEELGLEKKWQMNASCVVECPVNCQLSDWSSWSECSHTCGLAGKLWRRRTVIQASQGDGRPCSSQLEQWKPCPVKPCFSWRYSVWSPCKSEGARCGEGLRFRNVSCFVSDGSGKDAGSMVDEELCGDLEQTVDGDKQIILQESCTVPCPGECYLTDWTMWSPCQLSCIGGDDLGFGSVQVRSRAVLAQEPENLLQCPEQEWEARPCTEGQCYDYKWMTGAWRGSSRQVWCQRSDGLNVTGGCQSTTEPVSDRSCDPACDKPRSICTEAGICGCEEGYTEVMTSDGVLDQCTVIPVLEIPTAGDSKADVKTIRALNPTEPTANMPGRAGRTWFLQPFGPDGKLKTWVYGVAAGAFVLLVFIVSMTYLACKKPKKPQRRQMNNRLKPLTLAYDGDADM

Target ORF information:

RefSeq Version	XM_005159680.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159680.4

ORF Insert Sequence:

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGCAAAAAG 
CCAAAGAAGC CCCAGAGACG ACAGATGAAC AACAGACTCA AGCCTCTGAC TCTGGCTTAT 
GACGGCGATG CTGACATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7aa ( XM_009294351.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292626.1 Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X1, mRNA.

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CloneID	ODa42716
Clone ID Related Accession (Same CDS sequence)	XM_009294351.2 , XM_009294351.3
Accession Version	XM_009294351.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGTTTGTGC 
ATGCTGTCCC ATCGCTCTCC TCCGAGCCCC GACCCGTCCA TCTCCTCTGT GGTTTGGGTC 
GGACCCAATG ATCATGAGCT GATATACACC TGTCCTGACT GTCAGCGCAC TGAGACTCAG 
TTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292626.1
CDS	366..5474
Translation	MGLASRAPGKGGTSAGALASLFRVALLFFGLSDVQTQTVANTRPTYIWQTGPWGRCMGSECGPGGSQSRAVWCAHSEGWTTLHTNCQQSERPSNQQSCFRVCDWHKELYDWQLGAWNQCVPVSMRNAGVPRPAVCTRGEEGIQTREVGCVHKSDGVPAEDAICEYFEPKPRLEQACLIPCPRDCVVSEFSPWTSCSKSCGMGLQNRLRSVLAPPLFGGSACPNLTEFRTCQPGKCEGVESLHSLRVGPWGQCMASPIRQARDTGEARVPKAERKAKRDRQARQERQGKRRKNKEKKELRESKGERVRERVKEKKRMRDPETRELIKKKRTRNRQNRQGLKFWDLQVGYQTREVTCVHRSGSTASISQCTQQTLPVTYQACVISKDCEVSEWSDWSVCSKECYDLNGRKGQRTRTRQVQQFPVGGGAECPELEESEPCSPQGEGIPPCVVYNWRSTEWSDCRVDVLLSQQDRRRSNQTGLCGGGVQTREVYCVQAPSETSSNLGSLKSKDALRPVNSDLCLGVPHNTTQLCHIPCPVECEVSAWSAWGPCTFENCQDQSTKKGFKLRKRKIMNEPTGGTGNCPHLTEAIPCEEPSCYDWLLVKLEECVPDNDKVCGPGTQNPQVQCINSDGEFVDRQLCRDAILPMPVLCEVSCPKDCVLSPWTSWSLCSNTCSGKNSEGKQTRARSILAYNAGDGGVQCPNSSALQEVRSCNDHPCTVYHWQTGPWGQCIEDTSVPSANSSISRAVPGTAVNDAFCSVGMQTRKVICVRVNVGQVPPKKCPESLRPETVRPCLLPCKRDCVVTPYSDWTPCPSICQTGGSVKKKQSRKRIIIQLPANGGQDCPEVLFQEKDCDASSVCPGYRWKTHKWRRCQLVPWSIRQDSPGAQETCGPGLQARAVSCKKLDSGPADVAACLKFAGPMPQLTQSCQLPCQDDCQLTAWSKFSTCAADCVGVRTRKRTLVGKSKKREQCKNTQMYPLSETQYCPCNKYNAQPVGNWSDCILPEGRVEGLLGMKVQGDIKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCLSDCSQYVWVAEPWSVWKVSNVDLKENCGEGVQTRKVRCMQNTVDGPSDPVEDYLCDPEEMPLGARESKLPCPEDCVLTDWGSWSRCPLPCNVNSTRQRSASPIRQPSERKQCPSTTEKEICTLNSNCFHYSYNITDWSTCQLSERAVCGVGFKTRMLDCVRSDSKSVDLKFCEELGLEKKWQMNASCVVECPVNCQLSDWSSWSECSHTCGLAGKLWRRRTVIQASQGDGRPCSSQLEQWKPCPVKPCFSWRYSVWSPCKSEGARCGEGLRFRNVSCFVSDGSGKDAGSMVDEELCGDLEQTVDGDKQIILQESCTVPCPGECYLTDWTMWSPCQLSCIGGDDLGFGSVQVRSRAVLAQEPENLLQCPEQEWEARPCTEGQCYDYKWMTGAWRGSSRQVWCQRSDGLNVTGGCQSTTEPVSDRSCDPACDKPRSICTEAGICGCEEGYTEVMTSDGVLDQCTVIPVLEIPTAGDSKADVKTIRALNPTEPTANMPGRAGRTWFLQPFGPDGKLKTWVYGVAAGAFVLLVFIVSMTYLACLCMLSHRSPPSPDPSISSVVWVGPNDHELIYTCPDCQRTETQFS

Target ORF information:

RefSeq Version	XM_009294351.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294351.2

ORF Insert Sequence:

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGTTTGTGC 
ATGCTGTCCC ATCGCTCTCC TCCGAGCCCC GACCCGTCCA TCTCCTCTGT GGTTTGGGTC 
GGACCCAATG ATCATGAGCT GATATACACC TGTCCTGACT GTCAGCGCAC TGAGACTCAG 
TTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7aa ( XM_009294351.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292626.1 Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X1, mRNA.

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CloneID	ODa42716
Clone ID Related Accession (Same CDS sequence)	XM_009294351.2 , XM_009294351.3
Accession Version	XM_009294351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGTTTGTGC 
ATGCTGTCCC ATCGCTCTCC TCCGAGCCCC GACCCGTCCA TCTCCTCTGT GGTTTGGGTC 
GGACCCAATG ATCATGAGCT GATATACACC TGTCCTGACT GTCAGCGCAC TGAGACTCAG 
TTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292626.1
CDS	364..5472
Translation	MGLASRAPGKGGTSAGALASLFRVALLFFGLSDVQTQTVANTRPTYIWQTGPWGRCMGSECGPGGSQSRAVWCAHSEGWTTLHTNCQQSERPSNQQSCFRVCDWHKELYDWQLGAWNQCVPVSMRNAGVPRPAVCTRGEEGIQTREVGCVHKSDGVPAEDAICEYFEPKPRLEQACLIPCPRDCVVSEFSPWTSCSKSCGMGLQNRLRSVLAPPLFGGSACPNLTEFRTCQPGKCEGVESLHSLRVGPWGQCMASPIRQARDTGEARVPKAERKAKRDRQARQERQGKRRKNKEKKELRESKGERVRERVKEKKRMRDPETRELIKKKRTRNRQNRQGLKFWDLQVGYQTREVTCVHRSGSTASISQCTQQTLPVTYQACVISKDCEVSEWSDWSVCSKECYDLNGRKGQRTRTRQVQQFPVGGGAECPELEESEPCSPQGEGIPPCVVYNWRSTEWSDCRVDVLLSQQDRRRSNQTGLCGGGVQTREVYCVQAPSETSSNLGSLKSKDALRPVNSDLCLGVPHNTTQLCHIPCPVECEVSAWSAWGPCTFENCQDQSTKKGFKLRKRKIMNEPTGGTGNCPHLTEAIPCEEPSCYDWLLVKLEECVPDNDKVCGPGTQNPQVQCINSDGEFVDRQLCRDAILPMPVLCEVSCPKDCVLSPWTSWSLCSNTCSGKNSEGKQTRARSILAYNAGDGGVQCPNSSALQEVRSCNDHPCTVYHWQTGPWGQCIEDTSVPSANSSISRAVPGTAVNDAFCSVGMQTRKVICVRVNVGQVPPKKCPESLRPETVRPCLLPCKRDCVVTPYSDWTPCPSICQTGGSVKKKQSRKRIIIQLPANGGQDCPEVLFQEKDCDASSVCPGYRWKTHKWRRCQLVPWSIRQDSPGAQETCGPGLQARAVSCKKLDSGPADVAACLKFAGPMPQLTQSCQLPCQDDCQLTAWSKFSTCAADCVGVRTRKRTLVGKSKKREQCKNTQMYPLSETQYCPCNKYNAQPVGNWSDCILPEGRVEGLLGMKVQGDIKECGQGYRYQAMVCYDQDNRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKSCGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCLSDCSQYVWVAEPWSVWKVSNVDLKENCGEGVQTRKVRCMQNTVDGPSDPVEDYLCDPEEMPLGARESKLPCPEDCVLTDWGSWSRCPLPCNVNSTRQRSASPIRQPSERKQCPSTTEKEICTLNSNCFHYSYNITDWSTCQLSERAVCGVGFKTRMLDCVRSDSKSVDLKFCEELGLEKKWQMNASCVVECPVNCQLSDWSSWSECSHTCGLAGKLWRRRTVIQASQGDGRPCSSQLEQWKPCPVKPCFSWRYSVWSPCKSEGARCGEGLRFRNVSCFVSDGSGKDAGSMVDEELCGDLEQTVDGDKQIILQESCTVPCPGECYLTDWTMWSPCQLSCIGGDDLGFGSVQVRSRAVLAQEPENLLQCPEQEWEARPCTEGQCYDYKWMTGAWRGSSRQVWCQRSDGLNVTGGCQSTTEPVSDRSCDPACDKPRSICTEAGICGCEEGYTEVMTSDGVLDQCTVIPVLEIPTAGDSKADVKTIRALNPTEPTANMPGRAGRTWFLQPFGPDGKLKTWVYGVAAGAFVLLVFIVSMTYLACLCMLSHRSPPSPDPSISSVVWVGPNDHELIYTCPDCQRTETQFS

Target ORF information:

RefSeq Version	XM_009294351.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Aa (thsd7aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294351.3

ORF Insert Sequence:

ATGGGGCTGG CCAGCAGGGC GCCAGGCAAA GGGGGCACTT CAGCAGGGGC TCTTGCTTCC 
CTCTTCAGAG TAGCTCTGCT CTTTTTTGGG CTTTCGGATG TCCAGACACA AACAGTCGCT 
AACACAAGAC CCACCTACAT CTGGCAAACA GGACCATGGG GAAGGTGTAT GGGTAGTGAG 
TGTGGTCCGG GAGGAAGTCA AAGCCGAGCA GTATGGTGTG CCCACTCTGA AGGCTGGACA 
ACGTTACACA CCAACTGCCA ACAGTCGGAG CGGCCCAGCA ACCAACAGAG CTGCTTCCGG 
GTGTGTGATT GGCACAAAGA GCTGTATGAC TGGCAACTAG GGGCCTGGAA CCAATGTGTC 
CCGGTGTCCA TGCGCAATGC TGGCGTGCCA AGGCCAGCGG TGTGCACTCG GGGCGAAGAG 
GGCATCCAGA CGCGGGAAGT GGGCTGTGTG CACAAGTCGG ACGGCGTCCC GGCGGAAGAT 
GCCATCTGTG AGTACTTTGA GCCCAAGCCA CGTTTAGAGC AGGCCTGCCT TATCCCGTGT 
CCACGTGACT GTGTTGTGTC CGAGTTTTCT CCATGGACGT CCTGTTCTAA GAGTTGTGGG 
ATGGGGCTCC AGAACCGCTT GCGTTCGGTT CTTGCTCCTC CACTTTTCGG GGGTTCGGCT 
TGCCCTAATC TTACGGAGTT TCGCACTTGC CAGCCGGGCA AGTGCGAGGG TGTGGAGAGC 
CTGCACAGTT TGAGAGTGGG ACCTTGGGGT CAATGCATGG CTTCACCTAT AAGACAAGCC 
AGGGATACAG GAGAAGCCAG AGTGCCCAAA GCAGAACGGA AAGCTAAGCG GGATCGGCAG 
GCCAGACAAG AGCGTCAGGG AAAACGGCGC AAGAATAAGG AGAAAAAGGA GTTGAGAGAA 
AGCAAAGGAG AACGCGTGAG GGAAAGGGTC AAGGAGAAGA AGAGGATGAG GGATCCAGAG 
ACCCGAGAGC TCATCAAGAA GAAGAGGACG AGGAACCGTC AGAACCGACA GGGACTTAAG 
TTTTGGGACC TGCAGGTGGG CTACCAGACT CGGGAGGTGA CCTGTGTGCA TCGAAGTGGA 
AGCACAGCAT CAATCAGCCA GTGCACCCAG CAAACTCTTC CAGTGACATA TCAGGCCTGT 
GTGATCTCCA AGGACTGTGA AGTGAGCGAG TGGTCAGACT GGTCTGTCTG CTCGAAGGAG 
TGTTACGATC TGAACGGCCG AAAGGGCCAG CGGACGCGCA CCCGTCAAGT GCAGCAGTTC 
CCTGTGGGTG GAGGAGCCGA GTGTCCCGAG CTGGAGGAGA GCGAACCTTG CTCACCACAA 
GGAGAAGGCA TCCCGCCGTG TGTCGTGTAT AATTGGAGAA GCACAGAGTG GAGCGACTGC 
AGGGTGGATG TGCTCCTCAG CCAACAGGAC CGCCGCAGGA GCAATCAGAC CGGGCTCTGT 
GGAGGAGGAG TTCAGACAAG AGAGGTCTAC TGTGTTCAGG CCCCAAGTGA AACCTCATCC 
AACCTCGGCT CCCTCAAGAG CAAAGACGCC TTGCGTCCAG TAAACAGTGA CCTGTGTTTG 
GGTGTTCCTC ATAACACCAC CCAGCTCTGC CATATCCCCT GTCCTGTGGA GTGTGAGGTG 
TCCGCCTGGA GCGCATGGGG GCCCTGCACC TTCGAAAACT GCCAGGATCA ATCCACAAAG 
AAAGGTTTCA AACTGAGGAA AAGGAAAATC ATGAATGAGC CCACAGGTGG CACAGGGAAT 
TGCCCACACC TGACGGAGGC CATTCCCTGC GAGGAGCCCA GTTGCTACGA CTGGCTGCTG 
GTGAAGCTGG AGGAGTGTGT GCCTGACAAT GACAAAGTGT GTGGACCAGG AACCCAAAAC 
CCACAAGTCC AGTGCATTAA CAGTGACGGT GAATTTGTGG ACCGGCAGCT GTGTCGCGAT 
GCCATACTTC CCATGCCTGT GCTGTGTGAA GTGTCCTGTC CTAAAGACTG TGTGCTCAGT 
CCCTGGACGT CCTGGTCTCT ATGCTCCAAC ACCTGCTCAG GGAAGAACTC AGAGGGGAAG 
CAGACCAGAG CGCGCTCCAT CCTGGCCTAC AATGCTGGAG ATGGTGGAGT TCAGTGCCCC 
AACAGCAGTG CCTTGCAGGA GGTGCGCAGC TGCAATGATC ACCCCTGCAC TGTGTACCAC 
TGGCAGACGG GGCCCTGGGG CCAGTGCATA GAGGACACCT CCGTCCCGTC CGCCAACAGC 
TCTATCAGCC GGGCCGTACC AGGAACTGCT GTTAATGATG CCTTCTGCTC AGTCGGCATG 
CAGACGCGGA AAGTCATCTG TGTCCGGGTC AATGTGGGCC AGGTGCCGCC TAAAAAGTGT 
CCAGAGAGTC TGCGTCCAGA GACAGTAAGA CCGTGTCTTC TGCCCTGCAA GAGAGACTGT 
GTGGTCACTC CATACAGTGA CTGGACTCCC TGCCCATCTA TCTGCCAGAC AGGTGGTTCT 
GTGAAAAAAA AGCAATCAAG GAAACGCATT ATCATTCAGC TTCCGGCAAA CGGTGGCCAA 
GACTGTCCAG AAGTGCTCTT CCAGGAGAAG GACTGTGATG CTTCATCTGT TTGTCCAGGC 
TACAGGTGGA AAACCCACAA GTGGCGCAGG TGTCAACTGG TGCCTTGGTC CATTCGCCAG 
GACAGTCCCG GAGCACAAGA GACTTGTGGA CCAGGGCTAC AAGCCAGAGC CGTCTCGTGT 
AAAAAACTAG ATAGTGGACC AGCAGATGTG GCGGCGTGTC TGAAGTTTGC AGGTCCAATG 
CCTCAACTCA CTCAGTCCTG CCAGCTTCCA TGTCAGGACG ACTGCCAGCT GACCGCCTGG 
TCCAAGTTCT CCACATGCGC CGCTGATTGT GTGGGCGTCA GAACCAGGAA AAGGACTCTA 
GTTGGTAAAA GCAAAAAGAG GGAGCAGTGT AAAAACACAC AGATGTATCC TCTGAGCGAG 
ACGCAGTACT GTCCGTGTAA CAAGTACAAC GCTCAGCCGG TGGGCAACTG GTCTGACTGC 
ATCCTGCCTG AAGGACGTGT GGAGGGTCTG CTGGGGATGA AGGTTCAGGG CGATATCAAG 
GAGTGCGGTC AGGGTTATCG CTATCAGGCT ATGGTGTGCT ATGACCAGGA CAACCGACTC 
GTGGAGACGT CACGCTGCAA CAGTCATGGT TACATCGAGG AAGCATGCAT CATCCCCTGC 
CCATCAGACT GTAAACTCAG CGAATGGTCA AACTGGTCCC GGTGCAGTAA ATCCTGTGGC 
AGTGGAGTCA AAGTACGGTC CAAGTGGCTT CGGGAAAAAC CTTATAACGG CGGGAGGCCT 
TGTCCCAAAC TAGACCATGT GAATCAGGCT CAGGTGTATG AAGTGGTTCC GTGTCTGAGT 
GACTGCAGTC AGTATGTCTG GGTTGCTGAG CCATGGAGCG TATGGAAAGT CAGCAATGTC 
GATCTGAAGG AGAACTGTGG AGAAGGTGTA CAGACTAGGA AAGTCAGGTG CATGCAGAAC 
ACTGTTGATG GACCATCAGA CCCTGTTGAA GACTATCTGT GTGACCCAGA GGAGATGCCG 
CTTGGAGCCA GAGAAAGCAA ACTGCCCTGT CCTGAGGACT GCGTTCTGAC CGACTGGGGC 
TCCTGGAGTC GATGCCCGCT GCCGTGTAAT GTGAATAGTA CAAGGCAGAG GAGCGCCAGT 
CCTATCCGTC AGCCCAGTGA GAGGAAACAG TGTCCTTCCA CCACAGAGAA AGAAATATGC 
ACACTCAACT CCAACTGCTT CCATTACTCC TATAATATCA CAGACTGGAG CACGTGTCAA 
CTGAGTGAGC GAGCCGTGTG CGGGGTTGGA TTCAAGACGC GAATGCTGGA CTGTGTTCGC 
AGCGACAGCA AATCTGTGGA CCTCAAGTTT TGTGAAGAGC TCGGTCTGGA GAAGAAATGG 
CAGATGAACG CGTCTTGTGT GGTCGAGTGT CCTGTGAACT GCCAGCTGTC CGACTGGTCT 
TCTTGGTCTG AATGCTCTCA CACTTGTGGT CTTGCAGGGA AGCTGTGGAG AAGGAGGACT 
GTGATACAGG CGTCTCAGGG AGATGGACGA CCGTGTTCCT CTCAGCTGGA GCAGTGGAAA 
CCCTGTCCTG TCAAACCCTG CTTCAGCTGG AGGTACAGTG TCTGGTCCCC ATGCAAATCT 
GAGGGTGCAC GGTGTGGTGA AGGCCTTCGC TTCAGAAACG TGTCCTGCTT CGTGTCCGAT 
GGCTCAGGGA AGGATGCGGG CAGTATGGTG GATGAAGAAC TGTGTGGAGA TCTGGAGCAG 
ACAGTGGATG GAGACAAACA GATCATCCTG CAGGAGTCCT GCACTGTGCC CTGCCCAGGT 
GAGTGTTACC TGACAGACTG GACGATGTGG AGCCCTTGTC AACTCAGCTG TATCGGCGGG 
GATGATCTGG GCTTCGGGTC AGTGCAGGTT CGTTCTCGCG CTGTTCTCGC TCAGGAACCC 
GAAAACCTTC TTCAGTGTCC AGAACAAGAA TGGGAGGCCA GACCCTGTAC AGAGGGTCAG 
TGTTATGATT ACAAATGGAT GACCGGCGCA TGGAGGGGCT CGTCTCGACA AGTTTGGTGC 
CAGCGCTCGG ATGGATTAAA CGTCACAGGT GGATGCCAAT CGACGACCGA GCCGGTGTCA 
GACAGATCCT GTGACCCAGC CTGCGACAAG CCACGCTCCA TCTGCACTGA GGCCGGGATC 
TGCGGCTGTG AGGAAGGCTA CACTGAGGTC ATGACATCTG ATGGAGTTTT AGATCAGTGT 
ACGGTCATCC CAGTGCTGGA GATTCCCACC GCAGGAGACA GTAAAGCTGA CGTCAAGACC 
ATAAGAGCCC TGAACCCCAC GGAGCCCACC GCAAACATGC CCGGACGCGC CGGACGCACG 
TGGTTCCTGC AACCTTTTGG TCCCGATGGG AAACTGAAGA CCTGGGTTTA TGGTGTGGCA 
GCTGGAGCTT TTGTCCTGCT AGTGTTCATT GTCTCTATGA CTTACTTAGC CTGTTTGTGC 
ATGCTGTCCC ATCGCTCTCC TCCGAGCCCC GACCCGTCCA TCTCCTCTGT GGTTTGGGTC 
GGACCCAATG ATCATGAGCT GATATACACC TGTCCTGACT GTCAGCGCAC TGAGACTCAG 
TTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Heterologous Model of Thrombospondin Type 1 Domain-Containing 7A-Associated Membranous Nephropathy.
Journal of the American Society of Nephrology : JASN28(11)3262-3277(2017 Nov)
Tomas NM,Meyer-Schwesinger C,von Spiegel H,Kotb AM,Zahner G,Hoxha E,Helmchen U,Endlich N,Koch-Nolte F,Stahl RAK

Motor neuron-derived Thsd7a is essential for zebrafish vascular development via the Notch-dll4 signaling pathway.
Journal of biomedical science23(1)59(2016 Aug)
Liu LY,Lin MH,Lai ZY,Jiang JP,Huang YC,Jao LE,Chuang YJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish Thsd7a is a neural protein required for angiogenic patterning during development.
Developmental dynamics : an official publication of the American Association of Anatomists240(6)1412-21(2011 Jun)
Wang CH,Chen IH,Kuo MW,Su PT,Lai ZY,Wang CH,Huang WC,Hoffman J,Kuo CJ,You MS,Chuang YJ

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thsd7aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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