COL4A3 cDNA ORF clone, Danio rerio(Zebrafish)

The following COL4A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa55287 XM_009291834.2
Latest version!
Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa55286 XM_688921.9
Latest version!
Danio rerio collagen, type IV, alpha 3 (col4a3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
ODa55286 XM_688921.8 Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa55287
    Clone ID Related Accession (Same CDS sequence) XM_009291834.2
    Accession Version XM_009291834.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-5(IV) chain isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
    ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA
    GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT
    CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT
    ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT
    GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC
    CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG
    CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT
    GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT
    CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT
    GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA
    ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA
    GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA
    GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA
    GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG
    TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG
    GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA
    AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA
    GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA
    GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG
    CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC
    CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA
    CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA
    GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA
    GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT
    AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT
    CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT
    GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA
    GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT
    GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA
    GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA
    GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT
    CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT
    GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG
    TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA
    AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT
    CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC
    CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA
    ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT
    GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC
    TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC
    CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA
    CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT
    TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG
    GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA
    CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA
    AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT
    ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT
    AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC
    AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG
    GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT
    AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA
    CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290109.2
    CDS22..3204
    Translation

    Target ORF information:

    RefSeq Version XM_009291834.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291834.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
    ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA
    GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT
    CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT
    ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT
    GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC
    CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG
    CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT
    GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT
    CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT
    GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA
    ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA
    GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA
    GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA
    GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG
    TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG
    GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA
    AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA
    GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA
    GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG
    CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC
    CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA
    CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA
    GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA
    GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT
    AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT
    CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT
    GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA
    GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT
    GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA
    GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA
    GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT
    CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT
    GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG
    TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA
    AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT
    CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC
    CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA
    ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT
    GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC
    TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC
    CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA
    CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT
    TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG
    GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA
    CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA
    AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT
    ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT
    AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC
    AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG
    GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT
    AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA
    CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55286
    Clone ID Related Accession (Same CDS sequence) XM_688921.9 , XM_688921.8
    Accession Version XM_688921.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-5(IV) chain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_688921.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
    ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA
    GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT
    CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT
    ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT
    GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC
    CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG
    CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT
    GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT
    CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT
    GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA
    ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA
    GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA
    GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA
    GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG
    TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG
    GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA
    AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA
    GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA
    GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG
    CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC
    CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA
    CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA
    GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA
    GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT
    AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT
    CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT
    GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA
    GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT
    GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA
    GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA
    GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT
    CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT
    GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG
    TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA
    AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT
    CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC
    CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA
    ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT
    GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC
    TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC
    CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA
    CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT
    TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG
    GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA
    CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA
    AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT
    ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT
    AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC
    AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG
    GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT
    AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA
    CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT
    GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT
    GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG
    ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT
    CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT
    GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA
    AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG
    AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC
    CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT
    GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT
    GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG
    GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC
    CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA
    AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT
    GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT
    GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT
    CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA
    GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG
    CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT
    GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC
    ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC
    ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA
    ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT
    GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA
    ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT
    TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA
    CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA
    GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC
    CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC
    TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA
    ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA
    GGTGACCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_694013.8
    CDS57..5051
    Translation

    Target ORF information:

    RefSeq Version XM_688921.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type IV, alpha 3 (col4a3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_688921.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
    ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA
    GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT
    CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT
    ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT
    GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC
    CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG
    CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT
    GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT
    CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT
    GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA
    ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA
    GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA
    GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA
    GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG
    TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG
    GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA
    AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA
    GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA
    GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG
    CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC
    CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA
    CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA
    GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA
    GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT
    AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT
    CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT
    GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA
    GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT
    GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA
    GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA
    GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT
    CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT
    GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG
    TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA
    AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT
    CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC
    CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA
    ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT
    GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC
    TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC
    CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA
    CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT
    TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG
    GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA
    CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA
    AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT
    ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT
    AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC
    AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG
    GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT
    AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA
    CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT
    GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT
    GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG
    ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT
    CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT
    GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA
    AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG
    AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC
    CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT
    GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT
    GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG
    GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC
    CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA
    AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT
    GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT
    GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT
    CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA
    GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG
    CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT
    GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC
    ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC
    ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA
    ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT
    GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA
    ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT
    TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA
    CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA
    GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC
    CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC
    TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA
    ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA
    GGTGACCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55286
    Clone ID Related Accession (Same CDS sequence) XM_688921.9 , XM_688921.8
    Accession Version XM_688921.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-5(IV) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_688921.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
    ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA
    GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT
    CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT
    ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT
    GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC
    CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG
    CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT
    GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT
    CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT
    GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA
    ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA
    GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA
    GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA
    GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG
    TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG
    GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA
    AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA
    GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA
    GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG
    CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC
    CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA
    CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA
    GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA
    GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT
    AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT
    CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT
    GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA
    GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT
    GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA
    GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA
    GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT
    CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT
    GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG
    TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA
    AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT
    CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC
    CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA
    ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT
    GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC
    TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC
    CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA
    CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT
    TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG
    GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA
    CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA
    AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT
    ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT
    AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC
    AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG
    GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT
    AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA
    CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT
    GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT
    GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG
    ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT
    CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT
    GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA
    AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG
    AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC
    CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT
    GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT
    GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG
    GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC
    CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA
    AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT
    GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT
    GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT
    CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA
    GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG
    CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT
    GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC
    ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC
    ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA
    ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT
    GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA
    ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT
    TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA
    CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA
    GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC
    CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC
    TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA
    ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA
    GGTGACCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_694013.8
    CDS22..5016
    Translation

    Target ORF information:

    RefSeq Version XM_688921.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type IV, alpha 3 (col4a3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_688921.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGATCATT GGGTATTATT GCGCGTGACA CTTGTCGGAA TGATGCTGCT GGGACTTCTG 
    ACACCGACGC AAAGTAAAGG GGCTTGTGAT TGCAGTGGAA AATCAGGATG TGACTGCCAA
    GGAGTCAAAG GACAAAAGGG TGAGAAGGGT TCTCTTGGTT TGGTTGGTGA TGTTGGAGTT
    CAGGGGCATC AAGGAAAGGA AGGCCCTCCT GGATTTCCTG GTCTGAAGGG TTATTTGGGT
    ATACCAGGCA TCCCGGGACG TAAAGGTTCT AGGGGAATTT CAGGGACTCC TGGATTCTTT
    GGCCCTCCGG GACTACCGGG GCTTCCTGGG ATTCAGGGAC CAAGGGGGCC TCCTGGTCTC
    CCAGGATGCA ATGGAACAAA GGGTGAGCCT GGTCCTCCAG GTTTAATTGG GCCAATGGGG
    CGTCCTGGAA TCCAGGGAAG GCCAGGAATC CAAGGACCTA AGGGGGAGAC CATTTTTATT
    GCCGCTGCCG AACAAATGAT AGGACTTACG GGTCAACAAG GCAGAGCTGG AGATCCAGGT
    CCACCTGGGT TGCCAGGTGT TCAGGGAAAT ATTGGACCAG AGGGGCCTAA AGGTAACCCT
    GGACCACAAG GCATTCCTGG ATTTCCAGGA GAGAAGGGTA AAGAAGGTCG GAGCATGGGA
    ATACCTGGAC CCAGCGGTCA AAAGGGGGAG AGAGGACCCC CTGGTATACC TGGACAAAAA
    GGCATAAAGT TATTTGCATC AGGACCAAAG GAGCCTAAGG GTGACACTGG GGATTCTGGA
    GACAGAGGCA GCCCTGGAAT AATTGGTCTA CGAGGTCGTC CCGGTCCTAA GGGGGAGAAA
    GGGGAAAGAG GAGACCCTGG GGGTCCGGGT AAACCTGGAA AAGAGGGGCT ACAAGGAGAG
    TTAGGCTCTA AGGGAGGGAA GGGAGATAAA GGCTATCCCG GAGATACAGG CATAGAAGGG
    GAGAAGGGTC AGAAGGGTAA CCCTGGCCCC CCTGGTACGC CTGGAGAGTT CATTGTAATA
    AATACAGAGG TCAAAGGTCA AAAAGGGGAT CCTGGGCCAC CAGGACTTCT TGGTTTACGA
    GGTAGTGAAG GTTTCTATGG TTTTCCTGGA TATCAAGGAC TTCCAGGGAC CCCCATACAA
    GGACCTAATG GAAAACCAGG TCTACCAGGG TTTAAAGGTT TGAAAGGGGA TAAAGGAGAG
    CCTGGACCTG ACATCACAGG ACCCAAAGGT CGTGATGGCC AACGAGGGGA ACAAGGACCC
    CCTGGTGATC CTGGACTTCC GGGACCTTCA AATGTCATAA GCCGTTTGGG ACTCAAAGGA
    CTCAAGGGTG ATAAAGGTCT AATAGGCCCT GAGGGTCTGC GTGGGGATCC TGGGAACCAA
    GGAGAGCAAG GAGATCTGTG CAGGCTATGT GCTGCTTCTG CTGCTGATCC TGGTCCTCCA
    GGACAACCTG GGGATTCAGG CGAACCCGGC AAACCAGGTG CTGTTGGAAT TCAAGGTAAT
    AAAGGATCCA GAGGCTTGAA AGGTCCAATT GGTCCAGCTG GTCCTCCGGG TTTCTCAGGT
    CTTACAGGTT TTCCAGGGCC TTCAGGCCCC AAGGGAGAAA TGGGGATGAA ATATGAGATT
    GGGGAAAAAG GCTTTAAAGG AGACACTGGT GCAGTAGGAC TATTGGGATT AAGTGGACAA
    GATGGTTTAC CAGGCATCCC TGGCTTCAAA GGGCCACCGG GAGAAAAAGG AAAGCCAAGT
    GCTTTAGCTC CTAAAGGCGA TCAAGGATAT CCAGGTGTTG GTGGGGAACA AGGTAGCACA
    GGTCCAATGG GAAGTCAAGG GTCAATGGGT GTTGGGTCAC CAGGGGCACC TGGAGAAAAA
    GGAAATTTGG GAGTAGTGGG CCGGCAAGGA AATCTTGGAG ATCCAGGTGA AAAGGGATCT
    CCAGGACCAG TACAAACAAT ACCTGGAGAC CCAGGAGAGC CTGGACTGCC TGGTTCACCT
    GGTCTACCAG GACCTCCAGG TAATGAAGGT TGTGCTGGGG TTCAAGGATT CCCAGGATTG
    TGTGGGTTAA AAGGAGAGAA AGGACTCTCA ACACCAGGAG ATACTGGACT TACTGGTCCA
    AAAGGTGATA AGGGTGTGAA AGGGAGGCAA GGTTCACCTG GTTGTGGAAG TATAGGCCGT
    CCTGGACCTC CAGGGCCACC TGGAATGCCT GGCCCAAAGG GTGAAATAGC ATTTGGCTGC
    CCAGGGGCAA GGGGTAGAGA AGGTCTGCCA GGAGATGAAG GTTGCCTTGG TCCACCAGGA
    ATCTCTGGTC CTCCTGGATT ATCTGGATGT CGAGGTCAAA AAGGACAGGA TGGACCACCT
    GGAGAATTTG GTGTGCAGGG TTTTGATGGT TTTCCTGGAG TGGCTGGTGT TCCAGGATGC
    TGTATATCAG GGGCCAAAGG AGATGAAGGT CCCTCAGGAA TCAGAGGAGC AGATGGACCC
    CCTGGTTTGC CAGGTTTGAA AGGGCCACAA GGTGAAACTG GACTACCTGG ATTGAGCATA
    CCTGGTGTAA CAGGTGTGGT TGGGATACCT GGGATCTTTG GACCAGTTGG TGCAGTTGGT
    TTTCGGGGAC CAAAAGGTTT CCAGGGTCGA GATGGTTCCC CAGGATTTCC AGGTATACGG
    GGAGATCAGG GCAGTCCTGG AATGACTGGC CCACCTGGAG TAGATGGAAA TCCTGGACCA
    CCAGGGCTCC GGGGACCTAA AGGTTTAATG GGAAGGAATG GGGTCCCAGG TGATGAAGGA
    AAGCCTGGGG AGCCAGGAGA CCCTGGGCCC CGTGGCCCAA AGGGACAATC ATCAAATGTT
    ACGTCTCCAG GAGAACTTGG TCCTCCTGGC CTACCAGGTC TCCTTGGTCT GCCTGGTAGT
    AAAGGGGAAA TAGGACTTCC AGGCATTTGT GGGCAATGTG GGAAGAATGG CGTTCCAGGC
    AGTTCTGGTC CACCAGGCCC TCCTGGTGAC CCTGGCATAA AGGGGCTTCC TGGAAATCAG
    GGTCTTAATG GAAACTTTGG AGATCAAGGA GTTATGGGGT TTCCAGGAGT GAAAGGTGTT
    AAAGGTTCAC CTGGTTTTCA CGGGGAGCCT GGGATTCCTG GTGTTCCAGG ACCAAATGGA
    CCAAAGGGGA TCAAAGGTGA TCCTAGCCCC CCTGGGCCAG AGACTCCAGG ACTACTTGGT
    GAGAAGGGTG AACCAGGGTG TGCTGGGGGT ACAGGGAAGA AAGGTGACAA AGGTATATGT
    GGAATAAATG GGATCCCAGG TTTCTCTGGA GTACCTGGGG AAAGAGGAGT AACTGGAGTG
    ACAGGAGATC CAGGACCCCC AGGAGAAGCT GGTGATGATG GGTTTGATGG ATTTCCAGGT
    CCACCTGGAT GTGAAGGACC CTGTGGTCCA CCAGGTGTTC CTGGCTCGCC TGGAGATCCT
    GGACCTCCAG GACCTCGGGG AGAACAAGGC AGAGATGGCA TTCCAGGGTC AGCCGGAGAA
    AAAGGAGACA GAGGGTGCAC TATGACTGGG GATTCTGGCC TACCTGGCAT CAAGGGTGAG
    AAAGGCATTA AAGGCACCCG TGGACCACCA GGGCCATGTG GGCCACCAGG ACCCTGTGGC
    CCATCTGGAG AAATTGGTTC TCCAGGCAAA CCCGGAGAAA AAGGGCAACG TGGGTGTTCT
    GGACCACCTG GTGTCTGTAT TAGTGGCCCA AAAGGAGAGC GAGGACCTAA TGGGTTTCCT
    GGTCCACCAG GCCTCCAAGG ACCATGTGGG CCTCCTGGAG ACCCAGGTCC AGGCCTTAAG
    GGAGATAAAG GCTGTGTCGG GCCCCAAGGA CGCGTAGGAC CACCTGGTTG TACTGGTGAC
    CGGGGAACTC CAGGTGAACC AGGTAAAGAT GGAGATTGTG GTTTAAGGGG TCCAAAAGGA
    AATATAGGGC CTTTGGGTAG AGTTGGACCA GATGGACAAA AAGGACAGTG TGGTCCTGTT
    GGACCTGCTG GATTAAAAGG TTGTAAAGGA TCTCCTGGAA CTAAAGGGCC AAAAGGCTGT
    GATTACACTG TTGAGATCTT TTCTACACCA GGAGATCGCG GATTTCGTGG AAAAACCGGT
    CAGAAAGGAG AACCAGGGGA TAATGGAGAT AAAGGATTGC GAGGTCTAAA AGGAACAAAA
    GGTCAAAAAG GAGATATAGG AGCAAATGGA CCTGATGGAT CTAAGGGGAT GCTCGGGCTG
    CAGGGAGACA GTGGAGAAAA AGGACTAAAA GGTGCTCGGG GTTTGACAGG CGAAGTGGGT
    GCCAAGGGTG CACCAGGTGC AAAAGGTCCA CAACTGAGAA AAAGAGACGG ATTCCTTTTC
    ACCAGACATA GTCAGACAAC GGTTATACCA GAATGTCCTG CGGGGTCGAA ACGTTTATAC
    ACTGGATATT CACTTCTCTT CATCAACGGC AATAACCGAG GACATGGACA AGACCTAGGA
    ACTCTGGGAA GTTGTTTGCC GATGTTCAAC ACCATGCCTT TTATGGTGTG TAACCGTGAT
    GAAACTTGTC GCTACGCCTC TCGAAATGAC TACTCCTATT GGCTGTCAAC AGACACACCA
    ATGTTACCTG ACCAGCAAAT GATGTCTGGT GAAATACTGA AATGGTACAT CAGCAGGTGT
    TCCGTATGCG AGGCAATAGC TAATGTTATA GCAATTCACA GTCAGACCAT TAACATCCCA
    CAATGCCCTG TTGGGTGGCT TTCACTATGG GAAGGTTACT CCTTTGTAAT GCAAACAGGA
    GTTGGCGCAG AAGGTTCTGG GCAACCATTG GTCTCTCCTG GATCCTGTCT GGAACAATTC
    CGGAAGATTC CATTCATAGA GTGTCATGGT CGCGGCACCT GCAACTTCTA TCCTGATTCC
    TACAGCTACT GGCTTGCTTC ACTCGACCAC ACCAATATGT TCAGTATGCC AAACCGCCAA
    ACAGCAAAAC AGAAAGAAAT CATCAGCCGC TGTCAGGTCT GTATGAAGCA ACAGCCTCAA
    GGTGACCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.