gle1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gle1
Entrez Gene ID	445408
Full Name	GLE1 RNA export mediator homolog (yeast)
Synonyms	gle1l
General protein information	Preferred Names GLE1 RNA export mediator homolog (yeast) Names nucleoporin GLE1 GLE1-like protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166747.4	XP_005166804.1	nucleoporin GLE1 isoform X1
XM_005166747.3	XP_005166804.1	nucleoporin GLE1 isoform X1
NM_001003885.2	NP_001003885.2	nucleoporin GLE1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gle1	NP_083199.1
	GLE1	XP_001110844.1
Gallus gallus (chicken)	GLE1	XP_004945891.1
Homo sapiens (human)	GLE1	NP_001003722.1
Canis lupus familiaris (dog)	GLE1	XP_537825.3
Danio rerio (zebrafish)	gle1	NP_001003885.1
Xenopus tropicalis (tropical clawed frog)	gle1	XP_002935547.1
Pan troglodytes (chimpanzee)	GLE1	XP_001159347.1
Bos taurus (cattle)	GLE1	NP_001029890.1
Rattus norvegicus (Norway rat)	Gle1	NP_001020902.1

Gene Ontology
Bibliography

Related articles in PubMed

A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies.
Jao LE, Akef A, Wente SR
Molecular biology of the cell28(1)120-127(2017 Jan)

Deficiency in the mRNA export mediator Gle1 impairs Schwann cell development in the zebrafish embryo.
Seytanoglu A, Alsomali NI, Valori CF, McGown A, Kim HR, Ning K, Ramesh T, Sharrack B, Wood JD, Azzouz M
Neuroscience322287-97(2016 May)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate a critical requirement for normal gle1 gene function in Schwann cell development, thereby suggesting that myelination defects may be an important factor in lethal congenital contracture syndrome 1
Title: Deficiency in the mRNA export mediator Gle1 impairs Schwann cell development in the zebrafish embryo.

defective zebrafish GLE1 function in human LCCS1 results in both neurogenic and non-neurogenic defects linked to the apoptosis of proliferative organ precursors
Title: A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization.

The following gle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13801	XM_005166747.4 Latest version!	Danio rerio GLE1 RNA export mediator homolog (yeast) (gle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa53208	NM_001003885.2 Latest version!	Danio rerio GLE1 RNA export mediator (gle1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa13801	XM_005166747.3	Danio rerio GLE1 RNA export mediator homolog (yeast) (gle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gle1 ( XM_005166747.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166804.1 Danio rerio GLE1 RNA export mediator homolog (yeast) (gle1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13801
Clone ID Related Accession (Same CDS sequence)	XM_005166747.4 , XM_005166747.3
Accession Version	XM_005166747.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nucleoporin GLE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166747.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG AAAACCTTCG GTGGGAAACG TTAGAAGCGC TGAAAAACTC TCCGAAGGGG 
AAACTCAAAT ACAGCCCAGA CTGGGTGGAA AAAGGGGAGG ATGTTCTGGC CGGCTGTGTG 
GAGGTGCCCA GTCTGTCTCC TCTGTCAGGG CAGATCCTGA AGAGAATGAG TCCTCGAATG 
CTGCTCAAGA ACTGCTCTCG ATCCTCATCC GTCCGTGATA CGAGTCCCGG TCTTTCTGAA 
GAAGCTGCCG TCTGCAGATC AGCTCCCATC AGCCCCCGAC TGAAGCGTTC GTCCTGCTCT 
CTGCCTGCAG TCTCCATCCA AACTGAGGAG GAGGAGGAGA AGGAGAAGGA GAAAGCAGAA 
GTGGTTGCGG AGGCTCCTGC AGTGAGTCCT GAAGCATCGG TCAGTACTCC TCCGGCAGTC 
TCAGTCCTGT CTCCCAGAGC CACGCAGATC TCTGGTTGCA TCCGCATGTG TGAACAGAAA 
CACAAGGCCA AAGCCAAGAT GGAGCTGAGT CTCAGACAGG AGCAGCAGGA GCGTCTGGTG 
GCCACCGTTG CCAACCATGA GTCCGAGCAG CTGAAGCGCT TCGAGGAGTT CATGGAGCTC 
AAGCAGAGGC AGGAGCACCA GAGTATTAGA GACACCAATG AAAAAGAGGC GCAGGAGAGT 
CTCGGCCGCC AGGAGAAGCT GAGAGAGGAA CACAGGCACA GAATGAAAAT CCTGAACCTG 
CGTCTGCGTG AGATGGAGCA GCAGCGTCTG CGGGAGGTGG AGCTGGAGCG CCAGCGGCAG 
GTGGAGGGCA GAGAGCGGCA CAGAGCCATT AATGCCATCC AGGAGGAGGT TCTGCAGCTC 
AACCGGCTCC TGCAGCCGCA GCAGAGCACA CATGCGGAGG TGGAGCACGC GCCGTACATC 
ACACGGGGCA ACCAGCTCTG CTCACAGCTG TCTGAGGTGG TGCCGGCTGC AGCAGACGAT 
CAGTTTCCCA GCGTGGAGGA TTTGAGTGTG GCGGAGCGAG CTCTACAGGA AATGAGGTCA 
TTGGTCCGAA GCCTGCAGGA AGCAGTTTCG CAGGCTGCTG AGAGGAAGAA GAAGAAAGAA 
CAGGAGGAAG AGGAGGAGAA GAGGAGGCAG GAGCAGCTGA AAGCCCAGCA AGAGGAGCAG 
AAGAAGAGCG CTGCACTGTC GGCTAAAGAA AAAGCCAAAA AAGAGGGTCT GCAGACTGGA 
GCGGATGACA GCACTTTAAA GTGGTACAAC AGCCTGCAGG ATTTGGCCAA TCAATGCGCT 
CAGGCTTTCG ATGACCTCAA TAAAGCCAAA GACACACAGA CAAAAAAAAT TAAAATGGAG 
CTCCAAAAGG CTGCAACCAC ACCTGTGAGT CAGATCGCCA ACAGCTCAGG AGCTCCACTG 
AAGGAGGCCT TTGAGAAGAT TGATAAGCTT TTATCAGGAC GTCCCGTGAC ATCGGCTGGA 
AAGACAGTGT CTACATCCCA GCATCCTCAG GGGCTGGAGT TTGCCAGTTA TAGACTGGCA 
GAGAAGTTTG TGAAACAAGG AGAGGAGGAG GTTGCCTCTA ATCATTCGGC TGCTTTTCCC 
ATCGGCGCTG TGGCCTCAGG CATATGGGAA CTCCATCCCA AAATTGGAGA TCTTATACTC 
GCTCACCTGC ATAAGAAGTG CCCGTATGCT GTGCCGCACT ACCCACCCAT GGAGAGTGGC 
ACATCAGTGG AGGATTACCA GAAGATCCTT GGATATCGTG TAGATGAGGG GAAAGTGGAA 
GGACAGGACA GCTTTCTAAA GAGAATGTCG GGAATGATTC GCCTTTATGC TGCAATAATC 
CAGATGAGAT GGCCATACTC CAGCAAACAA GGGCCTCATC CTCATGGTCT GAATCACGGC 
TGGCGCTGGA TGGCACAGAT CCTCAACATG GAGCCGCTGG CCGACATCAC AGCCACTATT 
CTCTTTGACT TCCTGGAGGT CTGTGGAAAT GCCCTAATGA AACAATATCG CGTCCAGTTC 
TGGAAACTGA TATTGATTAT TAATGAAGAA TATTTCCCGA GGATTGAGGC TGTGACCAGC 
GCTGGACAGA TGGGCTCTGT TACCAGACTC AAACAGTTTC TAGAGACGTC ATTGAGAACC 
AAGCAGATTC CTGCTCCTAA GAGTGAGCTC GGCTCCGCCT TCTTCAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166804.1
CDS	113..2266
Translation	MPSENLRWETLEALKNSPKGKLKYSPDWVEKGEDVLAGCVEVPSLSPLSGQILKRMSPRMLLKNCSRSSSVRDTSPGLSEEAAVCRSAPISPRLKRSSCSLPAVSIQTEEEEEKEKEKAEVVAEAPAVSPEASVSTPPAVSVLSPRATQISGCIRMCEQKHKAKAKMELSLRQEQQERLVATVANHESEQLKRFEEFMELKQRQEHQSIRDTNEKEAQESLGRQEKLREEHRHRMKILNLRLREMEQQRLREVELERQRQVEGRERHRAINAIQEEVLQLNRLLQPQQSTHAEVEHAPYITRGNQLCSQLSEVVPAAADDQFPSVEDLSVAERALQEMRSLVRSLQEAVSQAAERKKKKEQEEEEEKRRQEQLKAQQEEQKKSAALSAKEKAKKEGLQTGADDSTLKWYNSLQDLANQCAQAFDDLNKAKDTQTKKIKMELQKAATTPVSQIANSSGAPLKEAFEKIDKLLSGRPVTSAGKTVSTSQHPQGLEFASYRLAEKFVKQGEEEVASNHSAAFPIGAVASGIWELHPKIGDLILAHLHKKCPYAVPHYPPMESGTSVEDYQKILGYRVDEGKVEGQDSFLKRMSGMIRLYAAIIQMRWPYSSKQGPHPHGLNHGWRWMAQILNMEPLADITATILFDFLEVCGNALMKQYRVQFWKLILIINEEYFPRIEAVTSAGQMGSVTRLKQFLETSLRTKQIPAPKSELGSAFFRS

Target ORF information:

RefSeq Version	XM_005166747.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLE1 RNA export mediator homolog (yeast) (gle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166747.4

ORF Insert Sequence:

ATGCCTTCTG AAAACCTTCG GTGGGAAACG TTAGAAGCGC TGAAAAACTC TCCGAAGGGG 
AAACTCAAAT ACAGCCCAGA CTGGGTGGAA AAAGGGGAGG ATGTTCTGGC CGGCTGTGTG 
GAGGTGCCCA GTCTGTCTCC TCTGTCAGGG CAGATCCTGA AGAGAATGAG TCCTCGAATG 
CTGCTCAAGA ACTGCTCTCG ATCCTCATCC GTCCGTGATA CGAGTCCCGG TCTTTCTGAA 
GAAGCTGCCG TCTGCAGATC AGCTCCCATC AGCCCCCGAC TGAAGCGTTC GTCCTGCTCT 
CTGCCTGCAG TCTCCATCCA AACTGAGGAG GAGGAGGAGA AGGAGAAGGA GAAAGCAGAA 
GTGGTTGCGG AGGCTCCTGC AGTGAGTCCT GAAGCATCGG TCAGTACTCC TCCGGCAGTC 
TCAGTCCTGT CTCCCAGAGC CACGCAGATC TCTGGTTGCA TCCGCATGTG TGAACAGAAA 
CACAAGGCCA AAGCCAAGAT GGAGCTGAGT CTCAGACAGG AGCAGCAGGA GCGTCTGGTG 
GCCACCGTTG CCAACCATGA GTCCGAGCAG CTGAAGCGCT TCGAGGAGTT CATGGAGCTC 
AAGCAGAGGC AGGAGCACCA GAGTATTAGA GACACCAATG AAAAAGAGGC GCAGGAGAGT 
CTCGGCCGCC AGGAGAAGCT GAGAGAGGAA CACAGGCACA GAATGAAAAT CCTGAACCTG 
CGTCTGCGTG AGATGGAGCA GCAGCGTCTG CGGGAGGTGG AGCTGGAGCG CCAGCGGCAG 
GTGGAGGGCA GAGAGCGGCA CAGAGCCATT AATGCCATCC AGGAGGAGGT TCTGCAGCTC 
AACCGGCTCC TGCAGCCGCA GCAGAGCACA CATGCGGAGG TGGAGCACGC GCCGTACATC 
ACACGGGGCA ACCAGCTCTG CTCACAGCTG TCTGAGGTGG TGCCGGCTGC AGCAGACGAT 
CAGTTTCCCA GCGTGGAGGA TTTGAGTGTG GCGGAGCGAG CTCTACAGGA AATGAGGTCA 
TTGGTCCGAA GCCTGCAGGA AGCAGTTTCG CAGGCTGCTG AGAGGAAGAA GAAGAAAGAA 
CAGGAGGAAG AGGAGGAGAA GAGGAGGCAG GAGCAGCTGA AAGCCCAGCA AGAGGAGCAG 
AAGAAGAGCG CTGCACTGTC GGCTAAAGAA AAAGCCAAAA AAGAGGGTCT GCAGACTGGA 
GCGGATGACA GCACTTTAAA GTGGTACAAC AGCCTGCAGG ATTTGGCCAA TCAATGCGCT 
CAGGCTTTCG ATGACCTCAA TAAAGCCAAA GACACACAGA CAAAAAAAAT TAAAATGGAG 
CTCCAAAAGG CTGCAACCAC ACCTGTGAGT CAGATCGCCA ACAGCTCAGG AGCTCCACTG 
AAGGAGGCCT TTGAGAAGAT TGATAAGCTT TTATCAGGAC GTCCCGTGAC ATCGGCTGGA 
AAGACAGTGT CTACATCCCA GCATCCTCAG GGGCTGGAGT TTGCCAGTTA TAGACTGGCA 
GAGAAGTTTG TGAAACAAGG AGAGGAGGAG GTTGCCTCTA ATCATTCGGC TGCTTTTCCC 
ATCGGCGCTG TGGCCTCAGG CATATGGGAA CTCCATCCCA AAATTGGAGA TCTTATACTC 
GCTCACCTGC ATAAGAAGTG CCCGTATGCT GTGCCGCACT ACCCACCCAT GGAGAGTGGC 
ACATCAGTGG AGGATTACCA GAAGATCCTT GGATATCGTG TAGATGAGGG GAAAGTGGAA 
GGACAGGACA GCTTTCTAAA GAGAATGTCG GGAATGATTC GCCTTTATGC TGCAATAATC 
CAGATGAGAT GGCCATACTC CAGCAAACAA GGGCCTCATC CTCATGGTCT GAATCACGGC 
TGGCGCTGGA TGGCACAGAT CCTCAACATG GAGCCGCTGG CCGACATCAC AGCCACTATT 
CTCTTTGACT TCCTGGAGGT CTGTGGAAAT GCCCTAATGA AACAATATCG CGTCCAGTTC 
TGGAAACTGA TATTGATTAT TAATGAAGAA TATTTCCCGA GGATTGAGGC TGTGACCAGC 
GCTGGACAGA TGGGCTCTGT TACCAGACTC AAACAGTTTC TAGAGACGTC ATTGAGAACC 
AAGCAGATTC CTGCTCCTAA GAGTGAGCTC GGCTCCGCCT TCTTCAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gle1 ( XM_005166747.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166804.1 Danio rerio GLE1 RNA export mediator homolog (yeast) (gle1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13801
Clone ID Related Accession (Same CDS sequence)	XM_005166747.4 , XM_005166747.3
Accession Version	XM_005166747.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleoporin GLE1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG AAAACCTTCG GTGGGAAACG TTAGAAGCGC TGAAAAACTC TCCGAAGGGG 
AAACTCAAAT ACAGCCCAGA CTGGGTGGAA AAAGGGGAGG ATGTTCTGGC CGGCTGTGTG 
GAGGTGCCCA GTCTGTCTCC TCTGTCAGGG CAGATCCTGA AGAGAATGAG TCCTCGAATG 
CTGCTCAAGA ACTGCTCTCG ATCCTCATCC GTCCGTGATA CGAGTCCCGG TCTTTCTGAA 
GAAGCTGCCG TCTGCAGATC AGCTCCCATC AGCCCCCGAC TGAAGCGTTC GTCCTGCTCT 
CTGCCTGCAG TCTCCATCCA AACTGAGGAG GAGGAGGAGA AGGAGAAGGA GAAAGCAGAA 
GTGGTTGCGG AGGCTCCTGC AGTGAGTCCT GAAGCATCGG TCAGTACTCC TCCGGCAGTC 
TCAGTCCTGT CTCCCAGAGC CACGCAGATC TCTGGTTGCA TCCGCATGTG TGAACAGAAA 
CACAAGGCCA AAGCCAAGAT GGAGCTGAGT CTCAGACAGG AGCAGCAGGA GCGTCTGGTG 
GCCACCGTTG CCAACCATGA GTCCGAGCAG CTGAAGCGCT TCGAGGAGTT CATGGAGCTC 
AAGCAGAGGC AGGAGCACCA GAGTATTAGA GACACCAATG AAAAAGAGGC GCAGGAGAGT 
CTCGGCCGCC AGGAGAAGCT GAGAGAGGAA CACAGGCACA GAATGAAAAT CCTGAACCTG 
CGTCTGCGTG AGATGGAGCA GCAGCGTCTG CGGGAGGTGG AGCTGGAGCG CCAGCGGCAG 
GTGGAGGGCA GAGAGCGGCA CAGAGCCATT AATGCCATCC AGGAGGAGGT TCTGCAGCTC 
AACCGGCTCC TGCAGCCGCA GCAGAGCACA CATGCGGAGG TGGAGCACGC GCCGTACATC 
ACACGGGGCA ACCAGCTCTG CTCACAGCTG TCTGAGGTGG TGCCGGCTGC AGCAGACGAT 
CAGTTTCCCA GCGTGGAGGA TTTGAGTGTG GCGGAGCGAG CTCTACAGGA AATGAGGTCA 
TTGGTCCGAA GCCTGCAGGA AGCAGTTTCG CAGGCTGCTG AGAGGAAGAA GAAGAAAGAA 
CAGGAGGAAG AGGAGGAGAA GAGGAGGCAG GAGCAGCTGA AAGCCCAGCA AGAGGAGCAG 
AAGAAGAGCG CTGCACTGTC GGCTAAAGAA AAAGCCAAAA AAGAGGGTCT GCAGACTGGA 
GCGGATGACA GCACTTTAAA GTGGTACAAC AGCCTGCAGG ATTTGGCCAA TCAATGCGCT 
CAGGCTTTCG ATGACCTCAA TAAAGCCAAA GACACACAGA CAAAAAAAAT TAAAATGGAG 
CTCCAAAAGG CTGCAACCAC ACCTGTGAGT CAGATCGCCA ACAGCTCAGG AGCTCCACTG 
AAGGAGGCCT TTGAGAAGAT TGATAAGCTT TTATCAGGAC GTCCCGTGAC ATCGGCTGGA 
AAGACAGTGT CTACATCCCA GCATCCTCAG GGGCTGGAGT TTGCCAGTTA TAGACTGGCA 
GAGAAGTTTG TGAAACAAGG AGAGGAGGAG GTTGCCTCTA ATCATTCGGC TGCTTTTCCC 
ATCGGCGCTG TGGCCTCAGG CATATGGGAA CTCCATCCCA AAATTGGAGA TCTTATACTC 
GCTCACCTGC ATAAGAAGTG CCCGTATGCT GTGCCGCACT ACCCACCCAT GGAGAGTGGC 
ACATCAGTGG AGGATTACCA GAAGATCCTT GGATATCGTG TAGATGAGGG GAAAGTGGAA 
GGACAGGACA GCTTTCTAAA GAGAATGTCG GGAATGATTC GCCTTTATGC TGCAATAATC 
CAGATGAGAT GGCCATACTC CAGCAAACAA GGGCCTCATC CTCATGGTCT GAATCACGGC 
TGGCGCTGGA TGGCACAGAT CCTCAACATG GAGCCGCTGG CCGACATCAC AGCCACTATT 
CTCTTTGACT TCCTGGAGGT CTGTGGAAAT GCCCTAATGA AACAATATCG CGTCCAGTTC 
TGGAAACTGA TATTGATTAT TAATGAAGAA TATTTCCCGA GGATTGAGGC TGTGACCAGC 
GCTGGACAGA TGGGCTCTGT TACCAGACTC AAACAGTTTC TAGAGACGTC ATTGAGAACC 
AAGCAGATTC CTGCTCCTAA GAGTGAGCTC GGCTCCGCCT TCTTCAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166804.1
CDS	97..2250
Translation	MPSENLRWETLEALKNSPKGKLKYSPDWVEKGEDVLAGCVEVPSLSPLSGQILKRMSPRMLLKNCSRSSSVRDTSPGLSEEAAVCRSAPISPRLKRSSCSLPAVSIQTEEEEEKEKEKAEVVAEAPAVSPEASVSTPPAVSVLSPRATQISGCIRMCEQKHKAKAKMELSLRQEQQERLVATVANHESEQLKRFEEFMELKQRQEHQSIRDTNEKEAQESLGRQEKLREEHRHRMKILNLRLREMEQQRLREVELERQRQVEGRERHRAINAIQEEVLQLNRLLQPQQSTHAEVEHAPYITRGNQLCSQLSEVVPAAADDQFPSVEDLSVAERALQEMRSLVRSLQEAVSQAAERKKKKEQEEEEEKRRQEQLKAQQEEQKKSAALSAKEKAKKEGLQTGADDSTLKWYNSLQDLANQCAQAFDDLNKAKDTQTKKIKMELQKAATTPVSQIANSSGAPLKEAFEKIDKLLSGRPVTSAGKTVSTSQHPQGLEFASYRLAEKFVKQGEEEVASNHSAAFPIGAVASGIWELHPKIGDLILAHLHKKCPYAVPHYPPMESGTSVEDYQKILGYRVDEGKVEGQDSFLKRMSGMIRLYAAIIQMRWPYSSKQGPHPHGLNHGWRWMAQILNMEPLADITATILFDFLEVCGNALMKQYRVQFWKLILIINEEYFPRIEAVTSAGQMGSVTRLKQFLETSLRTKQIPAPKSELGSAFFRS

Target ORF information:

RefSeq Version	XM_005166747.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GLE1 RNA export mediator homolog (yeast) (gle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166747.3

ORF Insert Sequence:

ATGCCTTCTG AAAACCTTCG GTGGGAAACG TTAGAAGCGC TGAAAAACTC TCCGAAGGGG 
AAACTCAAAT ACAGCCCAGA CTGGGTGGAA AAAGGGGAGG ATGTTCTGGC CGGCTGTGTG 
GAGGTGCCCA GTCTGTCTCC TCTGTCAGGG CAGATCCTGA AGAGAATGAG TCCTCGAATG 
CTGCTCAAGA ACTGCTCTCG ATCCTCATCC GTCCGTGATA CGAGTCCCGG TCTTTCTGAA 
GAAGCTGCCG TCTGCAGATC AGCTCCCATC AGCCCCCGAC TGAAGCGTTC GTCCTGCTCT 
CTGCCTGCAG TCTCCATCCA AACTGAGGAG GAGGAGGAGA AGGAGAAGGA GAAAGCAGAA 
GTGGTTGCGG AGGCTCCTGC AGTGAGTCCT GAAGCATCGG TCAGTACTCC TCCGGCAGTC 
TCAGTCCTGT CTCCCAGAGC CACGCAGATC TCTGGTTGCA TCCGCATGTG TGAACAGAAA 
CACAAGGCCA AAGCCAAGAT GGAGCTGAGT CTCAGACAGG AGCAGCAGGA GCGTCTGGTG 
GCCACCGTTG CCAACCATGA GTCCGAGCAG CTGAAGCGCT TCGAGGAGTT CATGGAGCTC 
AAGCAGAGGC AGGAGCACCA GAGTATTAGA GACACCAATG AAAAAGAGGC GCAGGAGAGT 
CTCGGCCGCC AGGAGAAGCT GAGAGAGGAA CACAGGCACA GAATGAAAAT CCTGAACCTG 
CGTCTGCGTG AGATGGAGCA GCAGCGTCTG CGGGAGGTGG AGCTGGAGCG CCAGCGGCAG 
GTGGAGGGCA GAGAGCGGCA CAGAGCCATT AATGCCATCC AGGAGGAGGT TCTGCAGCTC 
AACCGGCTCC TGCAGCCGCA GCAGAGCACA CATGCGGAGG TGGAGCACGC GCCGTACATC 
ACACGGGGCA ACCAGCTCTG CTCACAGCTG TCTGAGGTGG TGCCGGCTGC AGCAGACGAT 
CAGTTTCCCA GCGTGGAGGA TTTGAGTGTG GCGGAGCGAG CTCTACAGGA AATGAGGTCA 
TTGGTCCGAA GCCTGCAGGA AGCAGTTTCG CAGGCTGCTG AGAGGAAGAA GAAGAAAGAA 
CAGGAGGAAG AGGAGGAGAA GAGGAGGCAG GAGCAGCTGA AAGCCCAGCA AGAGGAGCAG 
AAGAAGAGCG CTGCACTGTC GGCTAAAGAA AAAGCCAAAA AAGAGGGTCT GCAGACTGGA 
GCGGATGACA GCACTTTAAA GTGGTACAAC AGCCTGCAGG ATTTGGCCAA TCAATGCGCT 
CAGGCTTTCG ATGACCTCAA TAAAGCCAAA GACACACAGA CAAAAAAAAT TAAAATGGAG 
CTCCAAAAGG CTGCAACCAC ACCTGTGAGT CAGATCGCCA ACAGCTCAGG AGCTCCACTG 
AAGGAGGCCT TTGAGAAGAT TGATAAGCTT TTATCAGGAC GTCCCGTGAC ATCGGCTGGA 
AAGACAGTGT CTACATCCCA GCATCCTCAG GGGCTGGAGT TTGCCAGTTA TAGACTGGCA 
GAGAAGTTTG TGAAACAAGG AGAGGAGGAG GTTGCCTCTA ATCATTCGGC TGCTTTTCCC 
ATCGGCGCTG TGGCCTCAGG CATATGGGAA CTCCATCCCA AAATTGGAGA TCTTATACTC 
GCTCACCTGC ATAAGAAGTG CCCGTATGCT GTGCCGCACT ACCCACCCAT GGAGAGTGGC 
ACATCAGTGG AGGATTACCA GAAGATCCTT GGATATCGTG TAGATGAGGG GAAAGTGGAA 
GGACAGGACA GCTTTCTAAA GAGAATGTCG GGAATGATTC GCCTTTATGC TGCAATAATC 
CAGATGAGAT GGCCATACTC CAGCAAACAA GGGCCTCATC CTCATGGTCT GAATCACGGC 
TGGCGCTGGA TGGCACAGAT CCTCAACATG GAGCCGCTGG CCGACATCAC AGCCACTATT 
CTCTTTGACT TCCTGGAGGT CTGTGGAAAT GCCCTAATGA AACAATATCG CGTCCAGTTC 
TGGAAACTGA TATTGATTAT TAATGAAGAA TATTTCCCGA GGATTGAGGC TGTGACCAGC 
GCTGGACAGA TGGGCTCTGT TACCAGACTC AAACAGTTTC TAGAGACGTC ATTGAGAACC 
AAGCAGATTC CTGCTCCTAA GAGTGAGCTC GGCTCCGCCT TCTTCAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gle1 ( NM_001003885.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003885.2 Danio rerio GLE1 RNA export mediator (gle1), mRNA.

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CloneID	ODa53208
Clone ID Related Accession (Same CDS sequence)	NM_001003885.2
Accession Version	NM_001003885.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-13
Organism	Danio rerio(zebrafish)
Product	nucleoporin GLE1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX088526.6. On Sep 5, 2015 this sequence version replaced NM_001003885.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AY648848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCTTCTG AAAACCTTCG GTGGGAAACG TTAGAAGCGC TGAAAAACTC TCCGAAGGGG 
AAACTCAAAT ACAGCCCAGA CTGGGTGGAA AAAGGGGAGG ATGTTCTGGC CGGCTGTGTG 
GAGGTGCCCA GTCTGTCTCC TCTGTCAGGG CAGATCCTGA AGAGAATGAG TCCTCGAATG 
CTGCTCAAGA ACTGCTCTCG ATCCTCATCC GTCCGTGATA CGAGTCCCGG TCTTTCTGAA 
GAAGCTGCCG TCTGCAGATC AGCTCCCATC AGCCCCCGAC TGAAGCGTTC GTCCTGCTCT 
CTGCCTGCAG TCTCCATCCA AACTGAGGAG GAGGAGGAGA AGGAGAAGGA GAAAGCAGAA 
GTGGTTGCGG AGGCTCCTGC AGTGAGTCCT GAAGCATCGG TCAGTACTCC TCCGGCAGTC 
TCAGTCCTGT CTCCCAGAGC CACGCAGATC TCTGGTTGCA TCCGCATGTG TGAACAGAAA 
CACAAGGCCA AAGCCAAGAT GGAGCTGAGT CTCAGACAGG AGCAGCAGGA GCGTCTGGTG 
GCCACCGTTG CCAACCATGA GTCCGAGCAG CTGAAGCGCT TCGAGGAGTT CATGGAGCTC 
AAGCAGAGGC AGGAGCACCA GAGTATTAGA GACACCAATG AAAAAGAGGC GCAGGAGAGT 
CTCGGCCGCC AGGAGAAGCT GAGAGAGGAA CACAGGCACA GAATGAAAAT CCTGAACCTG 
CGTCTGCGTG AGATGGAGCA GCAGCGTCTG CGGGAGGTGG AGCTGGAGCG CCAGCGGCAG 
GTGGAGGGCA GAGAGCGGCA CAGAGCCATT AATGCCATCC AGGAGGAGGT TCTGCAGCTC 
AACCGGCTCC TGCAGCCGCA GCAGAGCACA CATGCGGAGG TGGAGCACGC GCCGTACATC 
ACACGGGGCA ACCAGCTCTG CTCACAGCTG TCTGAGGTGG TGCCGGCTGC AGCAGACGAT 
CAGTTTCCCA GCGTGGAGGA TTTGAGTGTG GCGGAGCGAG CTCTACAGGA AATGAGGTCA 
TTGGTCCGAA GCCTGCAGGA AGCAGTTTCG CAGGCTGCTG AGAGGAAGAA GAAGAAAGAA 
CAGGAGGAAG AGGAGGAGAA GAGGAGGCAG GAGCAGCTGA AAGCCCAGCA AGAGGAGCAG 
AAGAAGAGCG CTGCACTGTC GGCTAAAGAA AAAGCCAAAA AAGAGGGTCT GCAGACTGGA 
GCGGATGACA GCACTTTAAA GTGGTACAAC AGCCTGCAGG ATTTGGCCAA TCAATGCGCT 
CAGGCTTTCG ATGACCTCAA TAAAGCCAAA GACACACAGA CAAAAAAAAT TAAAATGGAG 
CTCCAAAAGG CTGCAACCAC ACCTGTGAGT CAGATCGCCA ACAGCTCAGG AGCTCCACTG 
AAGGAGGCCT TTGAGAAGAT TGATAAGCTT TTATCAGGAC GTCCCGTGAC ATCGGCTGGA 
AAGACAGTGT CTACATCCCA GCATCCTCAG GGGCTGGAGT TTGCCAGTTA TAGACTGGCA 
GAGAAGTTTG TGAAACAAGG AGAGGAGGAG GTTGCCTCTA ATCATTCGGC TGCTTTTCCC 
ATCGGCGCTG TGGCCTCAGG CATATGGGAA CTCCATCCCA AAATTGGAGA TCTTATACTC 
GCTCACCTGC ATAAGAAGTG CCCGTATGCT GTGCCGCACT ACCCACCCAT GGAGAGTGGC 
ACATCAGTGG AGGATTACCA GAAGATCCTT GGATATCGTG TAGATGAGGG GAAAGTGGAA 
GGACAGGACA GCTTTCTAAA GAGAATGTCG GGAATGATTC GCCTTTATGC TGCAATAATC 
CAGATGAGAT GGCCATACTC CAGCAAACAA GGGCCTCATC CTCATGGTCT GAATCACGGC 
TGGCGCTGGA TGGCACAGAT CCTCAACATG GAGCCGCTGG CCGACATCAC AGCCACTATT 
CTCTTTGACT TCCTGGAGGT CTGTGGAAAT GCCCTAATGA AACAATATCG CGTCCAGTTC 
TGGAAACTGA TATTGATTAT TAATGAAGAA TATTTCCCGA GGTATTTATG CTTTGCTTCC 
ATATTACACT TTACATGCAT TCACTGGGTT CACAATATAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003885.2
CDS	102..2186
Translation	MPSENLRWETLEALKNSPKGKLKYSPDWVEKGEDVLAGCVEVPSLSPLSGQILKRMSPRMLLKNCSRSSSVRDTSPGLSEEAAVCRSAPISPRLKRSSCSLPAVSIQTEEEEEKEKEKAEVVAEAPAVSPEASVSTPPAVSVLSPRATQISGCIRMCEQKHKAKAKMELSLRQEQQERLVATVANHESEQLKRFEEFMELKQRQEHQSIRDTNEKEAQESLGRQEKLREEHRHRMKILNLRLREMEQQRLREVELERQRQVEGRERHRAINAIQEEVLQLNRLLQPQQSTHAEVEHAPYITRGNQLCSQLSEVVPAAADDQFPSVEDLSVAERALQEMRSLVRSLQEAVSQAAERKKKKEQEEEEEKRRQEQLKAQQEEQKKSAALSAKEKAKKEGLQTGADDSTLKWYNSLQDLANQCAQAFDDLNKAKDTQTKKIKMELQKAATTPVSQIANSSGAPLKEAFEKIDKLLSGRPVTSAGKTVSTSQHPQGLEFASYRLAEKFVKQGEEEVASNHSAAFPIGAVASGIWELHPKIGDLILAHLHKKCPYAVPHYPPMESGTSVEDYQKILGYRVDEGKVEGQDSFLKRMSGMIRLYAAIIQMRWPYSSKQGPHPHGLNHGWRWMAQILNMEPLADITATILFDFLEVCGNALMKQYRVQFWKLILIINEEYFPRYLCFASILHFTCIHWVHNIE

Target ORF information:

RefSeq Version	NM_001003885.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLE1 RNA export mediator (gle1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003885.2

ORF Insert Sequence:

ATGCCTTCTG AAAACCTTCG GTGGGAAACG TTAGAAGCGC TGAAAAACTC TCCGAAGGGG 
AAACTCAAAT ACAGCCCAGA CTGGGTGGAA AAAGGGGAGG ATGTTCTGGC CGGCTGTGTG 
GAGGTGCCCA GTCTGTCTCC TCTGTCAGGG CAGATCCTGA AGAGAATGAG TCCTCGAATG 
CTGCTCAAGA ACTGCTCTCG ATCCTCATCC GTCCGTGATA CGAGTCCCGG TCTTTCTGAA 
GAAGCTGCCG TCTGCAGATC AGCTCCCATC AGCCCCCGAC TGAAGCGTTC GTCCTGCTCT 
CTGCCTGCAG TCTCCATCCA AACTGAGGAG GAGGAGGAGA AGGAGAAGGA GAAAGCAGAA 
GTGGTTGCGG AGGCTCCTGC AGTGAGTCCT GAAGCATCGG TCAGTACTCC TCCGGCAGTC 
TCAGTCCTGT CTCCCAGAGC CACGCAGATC TCTGGTTGCA TCCGCATGTG TGAACAGAAA 
CACAAGGCCA AAGCCAAGAT GGAGCTGAGT CTCAGACAGG AGCAGCAGGA GCGTCTGGTG 
GCCACCGTTG CCAACCATGA GTCCGAGCAG CTGAAGCGCT TCGAGGAGTT CATGGAGCTC 
AAGCAGAGGC AGGAGCACCA GAGTATTAGA GACACCAATG AAAAAGAGGC GCAGGAGAGT 
CTCGGCCGCC AGGAGAAGCT GAGAGAGGAA CACAGGCACA GAATGAAAAT CCTGAACCTG 
CGTCTGCGTG AGATGGAGCA GCAGCGTCTG CGGGAGGTGG AGCTGGAGCG CCAGCGGCAG 
GTGGAGGGCA GAGAGCGGCA CAGAGCCATT AATGCCATCC AGGAGGAGGT TCTGCAGCTC 
AACCGGCTCC TGCAGCCGCA GCAGAGCACA CATGCGGAGG TGGAGCACGC GCCGTACATC 
ACACGGGGCA ACCAGCTCTG CTCACAGCTG TCTGAGGTGG TGCCGGCTGC AGCAGACGAT 
CAGTTTCCCA GCGTGGAGGA TTTGAGTGTG GCGGAGCGAG CTCTACAGGA AATGAGGTCA 
TTGGTCCGAA GCCTGCAGGA AGCAGTTTCG CAGGCTGCTG AGAGGAAGAA GAAGAAAGAA 
CAGGAGGAAG AGGAGGAGAA GAGGAGGCAG GAGCAGCTGA AAGCCCAGCA AGAGGAGCAG 
AAGAAGAGCG CTGCACTGTC GGCTAAAGAA AAAGCCAAAA AAGAGGGTCT GCAGACTGGA 
GCGGATGACA GCACTTTAAA GTGGTACAAC AGCCTGCAGG ATTTGGCCAA TCAATGCGCT 
CAGGCTTTCG ATGACCTCAA TAAAGCCAAA GACACACAGA CAAAAAAAAT TAAAATGGAG 
CTCCAAAAGG CTGCAACCAC ACCTGTGAGT CAGATCGCCA ACAGCTCAGG AGCTCCACTG 
AAGGAGGCCT TTGAGAAGAT TGATAAGCTT TTATCAGGAC GTCCCGTGAC ATCGGCTGGA 
AAGACAGTGT CTACATCCCA GCATCCTCAG GGGCTGGAGT TTGCCAGTTA TAGACTGGCA 
GAGAAGTTTG TGAAACAAGG AGAGGAGGAG GTTGCCTCTA ATCATTCGGC TGCTTTTCCC 
ATCGGCGCTG TGGCCTCAGG CATATGGGAA CTCCATCCCA AAATTGGAGA TCTTATACTC 
GCTCACCTGC ATAAGAAGTG CCCGTATGCT GTGCCGCACT ACCCACCCAT GGAGAGTGGC 
ACATCAGTGG AGGATTACCA GAAGATCCTT GGATATCGTG TAGATGAGGG GAAAGTGGAA 
GGACAGGACA GCTTTCTAAA GAGAATGTCG GGAATGATTC GCCTTTATGC TGCAATAATC 
CAGATGAGAT GGCCATACTC CAGCAAACAA GGGCCTCATC CTCATGGTCT GAATCACGGC 
TGGCGCTGGA TGGCACAGAT CCTCAACATG GAGCCGCTGG CCGACATCAC AGCCACTATT 
CTCTTTGACT TCCTGGAGGT CTGTGGAAAT GCCCTAATGA AACAATATCG CGTCCAGTTC 
TGGAAACTGA TATTGATTAT TAATGAAGAA TATTTCCCGA GGTATTTATG CTTTGCTTCC 
ATATTACACT TTACATGCAT TCACTGGGTT CACAATATAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies.
Molecular biology of the cell28(1)120-127(2017 Jan)
Jao LE,Akef A,Wente SR

Deficiency in the mRNA export mediator Gle1 impairs Schwann cell development in the zebrafish embryo.
Neuroscience322287-97(2016 May)
Seytanoglu A,Alsomali NI,Valori CF,McGown A,Kim HR,Ning K,Ramesh T,Sharrack B,Wood JD,Azzouz M

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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gle1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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