NELFE cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFE
Entrez Gene ID	436648
Full Name	negative elongation factor complex member E
Synonyms	rdbp,zgc:92496
General protein information	Preferred Names negative elongation factor complex member E Names negative elongation factor E RD RNA binding protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170027.4	XP_005170084.1	negative elongation factor E isoform X2
XM_005170026.3	XP_005170083.1	negative elongation factor E isoform X1
XM_021468046.1	XP_021323721.1	negative elongation factor E isoform X1
NM_001002375.1	NP_001002375.1	negative elongation factor E
XM_005170027.3	XP_005170084.1	negative elongation factor E isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RDBP	XP_003311227.1
Danio rerio (zebrafish)	nelfe	NP_001002375.1
Rattus norvegicus (Norway rat)	Nelfe	NP_001159373.1
Gallus gallus (chicken)	RDBP	XP_003642781.2

Gene Ontology
Bibliography

Related articles in PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P
Nucleic acids research45(16)9547-9557(2017 Sep)

RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Yang Q, Liu X, Zhou T, Cook J, Nguyen K, Bai X
Blood128(13)1701-10(2016 09)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP.
Liu J, Sun YH, Wang YW, Wang N, Pei DS, Wang YP, Hu W, Zhu ZY
Theriogenology70(9)1525-35(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NELFE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30524	NM_001002375.1 Latest version!	Danio rerio negative elongation factor complex member E (nelfe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
ODa24130	XM_021468046.1 Latest version!	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
ODa24130	XM_005170026.3 Latest version!	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
ODa24131	XM_005170027.4 Latest version!	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
ODa24131	XM_005170027.3	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFE ( NM_001002375.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001002375.1 Danio rerio negative elongation factor complex member E (nelfe), mRNA.

Price & Availability↑

CloneID	ODa30524
Clone ID Related Accession (Same CDS sequence)	NM_001002375.1
Accession Version	NM_001002375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	negative elongation factor E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076037.1. ##Evidence-Data-START## Transcript exon combination :: BC076037.1, AY391454.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAATAT TTCCCAGTTC ATTAACAGAA GACGAAGAAG CGCTGCAAAA GAAGTATGCT 
AAACTGAAGA AAAAGAAAAA GGCCCTAATG GCTCTGAAGA AACAGAGCTC CACCAATCAG 
ACGAGTCAGA CTGGACTGAA ACGCACTTTG TCAGATCAGC CGGTGGTGGA CACAGCGACA 
GCCACAGAGC AGGCCAAAAT GCTAGTCAAA TCTGGGGCAA TCAGTGCCAT CAAATCAGAA 
AACAAGAACT CGGGGTTCAA GCGCTCCAGA ATGCTTGAGG GTAAACTGAA AGATCCTGAG 
AAAGGTCCTG CCCCGGCATT CCTGCCATTT CAGAGGAGCG TGTCCACAGA TGAGGAGCCT 
CCTGATTCCG CTAAACGAAT CCACAGGAAA TCCCTTTACG AGAGTTTTGT TCCTGGAGAT 
CGATCTCGTG ATGAAGAGGC AGGAATGCCA AGTCGAGATT CTGAGCGTGA CCGGGAAAGG 
GAGATGGACT GGGAGAGAGA TCGAGAAAGA GACAGAGACA GAGAAAGGGA CCGTGAAAGA 
GGTCGAGAGA GAGAACGGGA CCGGGAACGG GAACGAGACA GGAGCCGGGA TGGAGAAAGA 
GATCGAGAGA GGGACCGGGA TCGAGACAGA GAACGAGACA GAGAGAGAGA TGGACCCTAC 
AGACGGTCAG ACTCGTATCC AGAGCGCAGA GGTGTGCGCA AAGGAAACAC AGTGTATGTG 
TACGGCACCG GTTTAGTGGA GGACAGCTTG CGCACAGCCT TTGCTCAGCA CGGCAACATC 
ATTGACCTTT CAATGGACTC TCCACGCAAT TGCGCTTTTG TCACCTTTGA GAAGATCGAA 
TCAGCAGACC AGGCTGTGGC GGAGCTGAAT GGCACCACTG TGGGTGACGT CAACATCAAA 
GTCAGCATTG CCAGGAAGCA GCCCATGCTG GATGCCGCCA CAGGGAAATC TGTCTGGGCT 
TCTCTTGCGG TGCAGAACAG TGCCAAGGGC TCGTACAGAG ACAAACGGAG CCAGGTCGTC 
TACAGTGAAG ATTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002375.1
CDS	60..1100
Translation	MAIFPSSLTEDEEALQKKYAKLKKKKKALMALKKQSSTNQTSQTGLKRTLSDQPVVDTATATEQAKMLVKSGAISAIKSENKNSGFKRSRMLEGKLKDPEKGPAPAFLPFQRSVSTDEEPPDSAKRIHRKSLYESFVPGDRSRDEEAGMPSRDSERDREREMDWERDRERDRDRERDRERGRERERDRERERDRSRDGERDRERDRDRDRERDRERDGPYRRSDSYPERRGVRKGNTVYVYGTGLVEDSLRTAFAQHGNIIDLSMDSPRNCAFVTFEKIESADQAVAELNGTTVGDVNIKVSIARKQPMLDAATGKSVWASLAVQNSAKGSYRDKRSQVVYSEDFL

Target ORF information:

RefSeq Version	NM_001002375.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio negative elongation factor complex member E (nelfe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002375.1

ORF Insert Sequence:

ATGGCAATAT TTCCCAGTTC ATTAACAGAA GACGAAGAAG CGCTGCAAAA GAAGTATGCT 
AAACTGAAGA AAAAGAAAAA GGCCCTAATG GCTCTGAAGA AACAGAGCTC CACCAATCAG 
ACGAGTCAGA CTGGACTGAA ACGCACTTTG TCAGATCAGC CGGTGGTGGA CACAGCGACA 
GCCACAGAGC AGGCCAAAAT GCTAGTCAAA TCTGGGGCAA TCAGTGCCAT CAAATCAGAA 
AACAAGAACT CGGGGTTCAA GCGCTCCAGA ATGCTTGAGG GTAAACTGAA AGATCCTGAG 
AAAGGTCCTG CCCCGGCATT CCTGCCATTT CAGAGGAGCG TGTCCACAGA TGAGGAGCCT 
CCTGATTCCG CTAAACGAAT CCACAGGAAA TCCCTTTACG AGAGTTTTGT TCCTGGAGAT 
CGATCTCGTG ATGAAGAGGC AGGAATGCCA AGTCGAGATT CTGAGCGTGA CCGGGAAAGG 
GAGATGGACT GGGAGAGAGA TCGAGAAAGA GACAGAGACA GAGAAAGGGA CCGTGAAAGA 
GGTCGAGAGA GAGAACGGGA CCGGGAACGG GAACGAGACA GGAGCCGGGA TGGAGAAAGA 
GATCGAGAGA GGGACCGGGA TCGAGACAGA GAACGAGACA GAGAGAGAGA TGGACCCTAC 
AGACGGTCAG ACTCGTATCC AGAGCGCAGA GGTGTGCGCA AAGGAAACAC AGTGTATGTG 
TACGGCACCG GTTTAGTGGA GGACAGCTTG CGCACAGCCT TTGCTCAGCA CGGCAACATC 
ATTGACCTTT CAATGGACTC TCCACGCAAT TGCGCTTTTG TCACCTTTGA GAAGATCGAA 
TCAGCAGACC AGGCTGTGGC GGAGCTGAAT GGCACCACTG TGGGTGACGT CAACATCAAA 
GTCAGCATTG CCAGGAAGCA GCCCATGCTG GATGCCGCCA CAGGGAAATC TGTCTGGGCT 
TCTCTTGCGG TGCAGAACAG TGCCAAGGGC TCGTACAGAG ACAAACGGAG CCAGGTCGTC 
TACAGTGAAG ATTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_005170026.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005170083.1 Danio rerio negative elongation factor complex member E (nelfe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24130
Clone ID Related Accession (Same CDS sequence)	XM_005170026.3 , XM_021468046.1
Accession Version	XM_005170026.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	negative elongation factor E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170026.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACAG ATTACGTCAT ATTTACGCGC CAGGTGAGGA TTTACTGCAG ACGCTCATCG 
CATAAAATTT ATTCTTTGAA TTTAGGGTCC AGCAAAATGG CAATATTTCC CAGTTCATTA 
ACAGAAGACG AAGAAGCGCT GCAAAAGAAG TATGCTAAAC TGAAGAAAAA GAAGAAGGCC 
CTAATGGCTT TGAAGAAACA GAGCTCCACC AATCAGACGA GTCAGACTGG ACTGAAACGC 
ACTTTGTCAG ATCAGCCGGT GGTGGACACA GCGACAGCCA CAGAGCAGGC CAAAATGCTA 
ATCAAATCTG GGGCAATCAG TGCCATCAAA TCAGAAAACA AGAACTCGGG GTTCAAGCGC 
TCCAGAATGC TTGAGGGTAA ACTGAAAGAT CCTGAGAAAG GTCCTGCCCC GGCATTCCTG 
CCATTTCAGA GGAGCGTGTC CACAGATGAG GAGCCTCCTG ATTCCGCTAA ACGAATCCAC 
AGGAAATCCC TTTACGAGAG TTTTGTTCCT GGAGATCGAT CTCGTGATGA AGAGGCAGGA 
ATGCCAAGTC GAGATTCTGA GCGTGACCGG GAAAGGGAGA TGGACTGGGA GAGAGATCGA 
GAAAGAGACA GAGACAGAGA AAGGGACCGT GAAAGAGGTC GAGAGAGAGA ACGGGACCGG 
GAACGGGAAC GAGACAGGAG CCGGGATGGA GAAAGAGATC GAGAGAGGGA CCGGGATCGA 
GACAGAGAAC GAGACAGAGA GAGAGATGGA CCCTACAGAC GGTCAGACTC GTATCCAGAG 
CGCAGAGGTG TGCGCAAAGG AAACACAGTG TATGTGTACG GCACCGGTTT AGTGGAGGAC 
AGCTTGCGCA CAGCCTTTGC TCAGCACGGC AACATCATTG ACCTTTCAAT GGACTCTCCA 
CGCAATTGCG CTTTTGTCAC CTTTGAGAAG ATCGAATCAG CAGACCAGGC TGTGGCGGAG 
CTGAATGGCA CCACTGTGGG TGACGTCAAC ATCAAAGTCA GCATTGCCAG GAAGCAGCCC 
ATGCTGGATG CCGCCACAGG GAAATCTGTC TGGGCTTCTC TTGCGGTGCA GAACAGTGCC 
AAGGGCTCGT ACAGAGACAA ACGGAGCCAG GTCGTCTACA GTGAAGATTT CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170083.1
CDS	1..1137
Translation	MTTDYVIFTRQVRIYCRRSSHKIYSLNLGSSKMAIFPSSLTEDEEALQKKYAKLKKKKKALMALKKQSSTNQTSQTGLKRTLSDQPVVDTATATEQAKMLIKSGAISAIKSENKNSGFKRSRMLEGKLKDPEKGPAPAFLPFQRSVSTDEEPPDSAKRIHRKSLYESFVPGDRSRDEEAGMPSRDSERDREREMDWERDRERDRDRERDRERGRERERDRERERDRSRDGERDRERDRDRDRERDRERDGPYRRSDSYPERRGVRKGNTVYVYGTGLVEDSLRTAFAQHGNIIDLSMDSPRNCAFVTFEKIESADQAVAELNGTTVGDVNIKVSIARKQPMLDAATGKSVWASLAVQNSAKGSYRDKRSQVVYSEDFL

Target ORF information:

RefSeq Version	XM_005170026.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170026.3

ORF Insert Sequence:

ATGACAACAG ATTACGTCAT ATTTACGCGC CAGGTGAGGA TTTACTGCAG ACGCTCATCG 
CATAAAATTT ATTCTTTGAA TTTAGGGTCC AGCAAAATGG CAATATTTCC CAGTTCATTA 
ACAGAAGACG AAGAAGCGCT GCAAAAGAAG TATGCTAAAC TGAAGAAAAA GAAGAAGGCC 
CTAATGGCTT TGAAGAAACA GAGCTCCACC AATCAGACGA GTCAGACTGG ACTGAAACGC 
ACTTTGTCAG ATCAGCCGGT GGTGGACACA GCGACAGCCA CAGAGCAGGC CAAAATGCTA 
ATCAAATCTG GGGCAATCAG TGCCATCAAA TCAGAAAACA AGAACTCGGG GTTCAAGCGC 
TCCAGAATGC TTGAGGGTAA ACTGAAAGAT CCTGAGAAAG GTCCTGCCCC GGCATTCCTG 
CCATTTCAGA GGAGCGTGTC CACAGATGAG GAGCCTCCTG ATTCCGCTAA ACGAATCCAC 
AGGAAATCCC TTTACGAGAG TTTTGTTCCT GGAGATCGAT CTCGTGATGA AGAGGCAGGA 
ATGCCAAGTC GAGATTCTGA GCGTGACCGG GAAAGGGAGA TGGACTGGGA GAGAGATCGA 
GAAAGAGACA GAGACAGAGA AAGGGACCGT GAAAGAGGTC GAGAGAGAGA ACGGGACCGG 
GAACGGGAAC GAGACAGGAG CCGGGATGGA GAAAGAGATC GAGAGAGGGA CCGGGATCGA 
GACAGAGAAC GAGACAGAGA GAGAGATGGA CCCTACAGAC GGTCAGACTC GTATCCAGAG 
CGCAGAGGTG TGCGCAAAGG AAACACAGTG TATGTGTACG GCACCGGTTT AGTGGAGGAC 
AGCTTGCGCA CAGCCTTTGC TCAGCACGGC AACATCATTG ACCTTTCAAT GGACTCTCCA 
CGCAATTGCG CTTTTGTCAC CTTTGAGAAG ATCGAATCAG CAGACCAGGC TGTGGCGGAG 
CTGAATGGCA CCACTGTGGG TGACGTCAAC ATCAAAGTCA GCATTGCCAG GAAGCAGCCC 
ATGCTGGATG CCGCCACAGG GAAATCTGTC TGGGCTTCTC TTGCGGTGCA GAACAGTGCC 
AAGGGCTCGT ACAGAGACAA ACGGAGCCAG GTCGTCTACA GTGAAGATTT CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_021468046.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323721.1 Danio rerio negative elongation factor complex member E (nelfe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24130
Clone ID Related Accession (Same CDS sequence)	XM_005170026.3 , XM_021468046.1
Accession Version	XM_021468046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	negative elongation factor E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACAG ATTACGTCAT ATTTACGCGC CAGGTGAGGA TTTACTGCAG ACGCTCATCG 
CATAAAATTT ATTCTTTGAA TTTAGGGTCC AGCAAAATGG CAATATTTCC CAGTTCATTA 
ACAGAAGACG AAGAAGCGCT GCAAAAGAAG TATGCTAAAC TGAAGAAAAA GAAGAAGGCC 
CTAATGGCTT TGAAGAAACA GAGCTCCACC AATCAGACGA GTCAGACTGG ACTGAAACGC 
ACTTTGTCAG ATCAGCCGGT GGTGGACACA GCGACAGCCA CAGAGCAGGC CAAAATGCTA 
ATCAAATCTG GGGCAATCAG TGCCATCAAA TCAGAAAACA AGAACTCGGG GTTCAAGCGC 
TCCAGAATGC TTGAGGGTAA ACTGAAAGAT CCTGAGAAAG GTCCTGCCCC GGCATTCCTG 
CCATTTCAGA GGAGCGTGTC CACAGATGAG GAGCCTCCTG ATTCCGCTAA ACGAATCCAC 
AGGAAATCCC TTTACGAGAG TTTTGTTCCT GGAGATCGAT CTCGTGATGA AGAGGCAGGA 
ATGCCAAGTC GAGATTCTGA GCGTGACCGG GAAAGGGAGA TGGACTGGGA GAGAGATCGA 
GAAAGAGACA GAGACAGAGA AAGGGACCGT GAAAGAGGTC GAGAGAGAGA ACGGGACCGG 
GAACGGGAAC GAGACAGGAG CCGGGATGGA GAAAGAGATC GAGAGAGGGA CCGGGATCGA 
GACAGAGAAC GAGACAGAGA GAGAGATGGA CCCTACAGAC GGTCAGACTC GTATCCAGAG 
CGCAGAGGTG TGCGCAAAGG AAACACAGTG TATGTGTACG GCACCGGTTT AGTGGAGGAC 
AGCTTGCGCA CAGCCTTTGC TCAGCACGGC AACATCATTG ACCTTTCAAT GGACTCTCCA 
CGCAATTGCG CTTTTGTCAC CTTTGAGAAG ATCGAATCAG CAGACCAGGC TGTGGCGGAG 
CTGAATGGCA CCACTGTGGG TGACGTCAAC ATCAAAGTCA GCATTGCCAG GAAGCAGCCC 
ATGCTGGATG CCGCCACAGG GAAATCTGTC TGGGCTTCTC TTGCGGTGCA GAACAGTGCC 
AAGGGCTCGT ACAGAGACAA ACGGAGCCAG GTCGTCTACA GTGAAGATTT CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323721.1
CDS	18..1154
Translation	MTTDYVIFTRQVRIYCRRSSHKIYSLNLGSSKMAIFPSSLTEDEEALQKKYAKLKKKKKALMALKKQSSTNQTSQTGLKRTLSDQPVVDTATATEQAKMLIKSGAISAIKSENKNSGFKRSRMLEGKLKDPEKGPAPAFLPFQRSVSTDEEPPDSAKRIHRKSLYESFVPGDRSRDEEAGMPSRDSERDREREMDWERDRERDRDRERDRERGRERERDRERERDRSRDGERDRERDRDRDRERDRERDGPYRRSDSYPERRGVRKGNTVYVYGTGLVEDSLRTAFAQHGNIIDLSMDSPRNCAFVTFEKIESADQAVAELNGTTVGDVNIKVSIARKQPMLDAATGKSVWASLAVQNSAKGSYRDKRSQVVYSEDFL

Target ORF information:

RefSeq Version	XM_021468046.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468046.1

ORF Insert Sequence:

ATGACAACAG ATTACGTCAT ATTTACGCGC CAGGTGAGGA TTTACTGCAG ACGCTCATCG 
CATAAAATTT ATTCTTTGAA TTTAGGGTCC AGCAAAATGG CAATATTTCC CAGTTCATTA 
ACAGAAGACG AAGAAGCGCT GCAAAAGAAG TATGCTAAAC TGAAGAAAAA GAAGAAGGCC 
CTAATGGCTT TGAAGAAACA GAGCTCCACC AATCAGACGA GTCAGACTGG ACTGAAACGC 
ACTTTGTCAG ATCAGCCGGT GGTGGACACA GCGACAGCCA CAGAGCAGGC CAAAATGCTA 
ATCAAATCTG GGGCAATCAG TGCCATCAAA TCAGAAAACA AGAACTCGGG GTTCAAGCGC 
TCCAGAATGC TTGAGGGTAA ACTGAAAGAT CCTGAGAAAG GTCCTGCCCC GGCATTCCTG 
CCATTTCAGA GGAGCGTGTC CACAGATGAG GAGCCTCCTG ATTCCGCTAA ACGAATCCAC 
AGGAAATCCC TTTACGAGAG TTTTGTTCCT GGAGATCGAT CTCGTGATGA AGAGGCAGGA 
ATGCCAAGTC GAGATTCTGA GCGTGACCGG GAAAGGGAGA TGGACTGGGA GAGAGATCGA 
GAAAGAGACA GAGACAGAGA AAGGGACCGT GAAAGAGGTC GAGAGAGAGA ACGGGACCGG 
GAACGGGAAC GAGACAGGAG CCGGGATGGA GAAAGAGATC GAGAGAGGGA CCGGGATCGA 
GACAGAGAAC GAGACAGAGA GAGAGATGGA CCCTACAGAC GGTCAGACTC GTATCCAGAG 
CGCAGAGGTG TGCGCAAAGG AAACACAGTG TATGTGTACG GCACCGGTTT AGTGGAGGAC 
AGCTTGCGCA CAGCCTTTGC TCAGCACGGC AACATCATTG ACCTTTCAAT GGACTCTCCA 
CGCAATTGCG CTTTTGTCAC CTTTGAGAAG ATCGAATCAG CAGACCAGGC TGTGGCGGAG 
CTGAATGGCA CCACTGTGGG TGACGTCAAC ATCAAAGTCA GCATTGCCAG GAAGCAGCCC 
ATGCTGGATG CCGCCACAGG GAAATCTGTC TGGGCTTCTC TTGCGGTGCA GAACAGTGCC 
AAGGGCTCGT ACAGAGACAA ACGGAGCCAG GTCGTCTACA GTGAAGATTT CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_005170027.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005170084.1 Danio rerio negative elongation factor complex member E (nelfe), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24131
Clone ID Related Accession (Same CDS sequence)	XM_005170027.4 , XM_005170027.3
Accession Version	XM_005170027.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	negative elongation factor E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170027.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATAT TTCCCAGTTC ATTAACAGAA GACGAAGAAG CGCTGCAAAA GAAGTATGCT 
AAACTGAAGA AAAAGAAGAA GGCCCTAATG GCTTTGAAGA AACAGAGCTC CACCAATCAG 
ACGAGTCAGA CTGGACTGAA ACGCACTTTG TCAGATCAGC CGGTGGTGGA CACAGCGACA 
GCCACAGAGC AGGCCAAAAT GCTAATCAAA TCTGGGGCAA TCAGTGCCAT CAAATCAGAA 
AACAAGAACT CGGGGTTCAA GCGCTCCAGA ATGCTTGAGG GTAAACTGAA AGATCCTGAG 
AAAGGTCCTG CCCCGGCATT CCTGCCATTT CAGAGGAGCG TGTCCACAGA TGAGGAGCCT 
CCTGATTCCG CTAAACGAAT CCACAGGAAA TCCCTTTACG AGAGTTTTGT TCCTGGAGAT 
CGATCTCGTG ATGAAGAGGC AGGAATGCCA AGTCGAGATT CTGAGCGTGA CCGGGAAAGG 
GAGATGGACT GGGAGAGAGA TCGAGAAAGA GACAGAGACA GAGAAAGGGA CCGTGAAAGA 
GGTCGAGAGA GAGAACGGGA CCGGGAACGG GAACGAGACA GGAGCCGGGA TGGAGAAAGA 
GATCGAGAGA GGGACCGGGA TCGAGACAGA GAACGAGACA GAGAGAGAGA TGGACCCTAC 
AGACGGTCAG ACTCGTATCC AGAGCGCAGA GGTGTGCGCA AAGGAAACAC AGTGTATGTG 
TACGGCACCG GTTTAGTGGA GGACAGCTTG CGCACAGCCT TTGCTCAGCA CGGCAACATC 
ATTGACCTTT CAATGGACTC TCCACGCAAT TGCGCTTTTG TCACCTTTGA GAAGATCGAA 
TCAGCAGACC AGGCTGTGGC GGAGCTGAAT GGCACCACTG TGGGTGACGT CAACATCAAA 
GTCAGCATTG CCAGGAAGCA GCCCATGCTG GATGCCGCCA CAGGGAAATC TGTCTGGGCT 
TCTCTTGCGG TGCAGAACAG TGCCAAGGGC TCGTACAGAG ACAAACGGAG CCAGGTCGTC 
TACAGTGAAG ATTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170084.1
CDS	178..1218
Translation	MAIFPSSLTEDEEALQKKYAKLKKKKKALMALKKQSSTNQTSQTGLKRTLSDQPVVDTATATEQAKMLIKSGAISAIKSENKNSGFKRSRMLEGKLKDPEKGPAPAFLPFQRSVSTDEEPPDSAKRIHRKSLYESFVPGDRSRDEEAGMPSRDSERDREREMDWERDRERDRDRERDRERGRERERDRERERDRSRDGERDRERDRDRDRERDRERDGPYRRSDSYPERRGVRKGNTVYVYGTGLVEDSLRTAFAQHGNIIDLSMDSPRNCAFVTFEKIESADQAVAELNGTTVGDVNIKVSIARKQPMLDAATGKSVWASLAVQNSAKGSYRDKRSQVVYSEDFL

Target ORF information:

RefSeq Version	XM_005170027.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170027.4

ORF Insert Sequence:

ATGGCAATAT TTCCCAGTTC ATTAACAGAA GACGAAGAAG CGCTGCAAAA GAAGTATGCT 
AAACTGAAGA AAAAGAAGAA GGCCCTAATG GCTTTGAAGA AACAGAGCTC CACCAATCAG 
ACGAGTCAGA CTGGACTGAA ACGCACTTTG TCAGATCAGC CGGTGGTGGA CACAGCGACA 
GCCACAGAGC AGGCCAAAAT GCTAATCAAA TCTGGGGCAA TCAGTGCCAT CAAATCAGAA 
AACAAGAACT CGGGGTTCAA GCGCTCCAGA ATGCTTGAGG GTAAACTGAA AGATCCTGAG 
AAAGGTCCTG CCCCGGCATT CCTGCCATTT CAGAGGAGCG TGTCCACAGA TGAGGAGCCT 
CCTGATTCCG CTAAACGAAT CCACAGGAAA TCCCTTTACG AGAGTTTTGT TCCTGGAGAT 
CGATCTCGTG ATGAAGAGGC AGGAATGCCA AGTCGAGATT CTGAGCGTGA CCGGGAAAGG 
GAGATGGACT GGGAGAGAGA TCGAGAAAGA GACAGAGACA GAGAAAGGGA CCGTGAAAGA 
GGTCGAGAGA GAGAACGGGA CCGGGAACGG GAACGAGACA GGAGCCGGGA TGGAGAAAGA 
GATCGAGAGA GGGACCGGGA TCGAGACAGA GAACGAGACA GAGAGAGAGA TGGACCCTAC 
AGACGGTCAG ACTCGTATCC AGAGCGCAGA GGTGTGCGCA AAGGAAACAC AGTGTATGTG 
TACGGCACCG GTTTAGTGGA GGACAGCTTG CGCACAGCCT TTGCTCAGCA CGGCAACATC 
ATTGACCTTT CAATGGACTC TCCACGCAAT TGCGCTTTTG TCACCTTTGA GAAGATCGAA 
TCAGCAGACC AGGCTGTGGC GGAGCTGAAT GGCACCACTG TGGGTGACGT CAACATCAAA 
GTCAGCATTG CCAGGAAGCA GCCCATGCTG GATGCCGCCA CAGGGAAATC TGTCTGGGCT 
TCTCTTGCGG TGCAGAACAG TGCCAAGGGC TCGTACAGAG ACAAACGGAG CCAGGTCGTC 
TACAGTGAAG ATTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NELFE ( XM_005170027.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005170084.1 Danio rerio negative elongation factor complex member E (nelfe), transcript variant X2, mRNA.

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CloneID	ODa24131
Clone ID Related Accession (Same CDS sequence)	XM_005170027.4 , XM_005170027.3
Accession Version	XM_005170027.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	negative elongation factor E isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATAT TTCCCAGTTC ATTAACAGAA GACGAAGAAG CGCTGCAAAA GAAGTATGCT 
AAACTGAAGA AAAAGAAGAA GGCCCTAATG GCTTTGAAGA AACAGAGCTC CACCAATCAG 
ACGAGTCAGA CTGGACTGAA ACGCACTTTG TCAGATCAGC CGGTGGTGGA CACAGCGACA 
GCCACAGAGC AGGCCAAAAT GCTAATCAAA TCTGGGGCAA TCAGTGCCAT CAAATCAGAA 
AACAAGAACT CGGGGTTCAA GCGCTCCAGA ATGCTTGAGG GTAAACTGAA AGATCCTGAG 
AAAGGTCCTG CCCCGGCATT CCTGCCATTT CAGAGGAGCG TGTCCACAGA TGAGGAGCCT 
CCTGATTCCG CTAAACGAAT CCACAGGAAA TCCCTTTACG AGAGTTTTGT TCCTGGAGAT 
CGATCTCGTG ATGAAGAGGC AGGAATGCCA AGTCGAGATT CTGAGCGTGA CCGGGAAAGG 
GAGATGGACT GGGAGAGAGA TCGAGAAAGA GACAGAGACA GAGAAAGGGA CCGTGAAAGA 
GGTCGAGAGA GAGAACGGGA CCGGGAACGG GAACGAGACA GGAGCCGGGA TGGAGAAAGA 
GATCGAGAGA GGGACCGGGA TCGAGACAGA GAACGAGACA GAGAGAGAGA TGGACCCTAC 
AGACGGTCAG ACTCGTATCC AGAGCGCAGA GGTGTGCGCA AAGGAAACAC AGTGTATGTG 
TACGGCACCG GTTTAGTGGA GGACAGCTTG CGCACAGCCT TTGCTCAGCA CGGCAACATC 
ATTGACCTTT CAATGGACTC TCCACGCAAT TGCGCTTTTG TCACCTTTGA GAAGATCGAA 
TCAGCAGACC AGGCTGTGGC GGAGCTGAAT GGCACCACTG TGGGTGACGT CAACATCAAA 
GTCAGCATTG CCAGGAAGCA GCCCATGCTG GATGCCGCCA CAGGGAAATC TGTCTGGGCT 
TCTCTTGCGG TGCAGAACAG TGCCAAGGGC TCGTACAGAG ACAAACGGAG CCAGGTCGTC 
TACAGTGAAG ATTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170084.1
CDS	178..1218
Translation	MAIFPSSLTEDEEALQKKYAKLKKKKKALMALKKQSSTNQTSQTGLKRTLSDQPVVDTATATEQAKMLIKSGAISAIKSENKNSGFKRSRMLEGKLKDPEKGPAPAFLPFQRSVSTDEEPPDSAKRIHRKSLYESFVPGDRSRDEEAGMPSRDSERDREREMDWERDRERDRDRERDRERGRERERDRERERDRSRDGERDRERDRDRDRERDRERDGPYRRSDSYPERRGVRKGNTVYVYGTGLVEDSLRTAFAQHGNIIDLSMDSPRNCAFVTFEKIESADQAVAELNGTTVGDVNIKVSIARKQPMLDAATGKSVWASLAVQNSAKGSYRDKRSQVVYSEDFL

Target ORF information:

RefSeq Version	XM_005170027.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio negative elongation factor complex member E (nelfe), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170027.3

ORF Insert Sequence:

ATGGCAATAT TTCCCAGTTC ATTAACAGAA GACGAAGAAG CGCTGCAAAA GAAGTATGCT 
AAACTGAAGA AAAAGAAGAA GGCCCTAATG GCTTTGAAGA AACAGAGCTC CACCAATCAG 
ACGAGTCAGA CTGGACTGAA ACGCACTTTG TCAGATCAGC CGGTGGTGGA CACAGCGACA 
GCCACAGAGC AGGCCAAAAT GCTAATCAAA TCTGGGGCAA TCAGTGCCAT CAAATCAGAA 
AACAAGAACT CGGGGTTCAA GCGCTCCAGA ATGCTTGAGG GTAAACTGAA AGATCCTGAG 
AAAGGTCCTG CCCCGGCATT CCTGCCATTT CAGAGGAGCG TGTCCACAGA TGAGGAGCCT 
CCTGATTCCG CTAAACGAAT CCACAGGAAA TCCCTTTACG AGAGTTTTGT TCCTGGAGAT 
CGATCTCGTG ATGAAGAGGC AGGAATGCCA AGTCGAGATT CTGAGCGTGA CCGGGAAAGG 
GAGATGGACT GGGAGAGAGA TCGAGAAAGA GACAGAGACA GAGAAAGGGA CCGTGAAAGA 
GGTCGAGAGA GAGAACGGGA CCGGGAACGG GAACGAGACA GGAGCCGGGA TGGAGAAAGA 
GATCGAGAGA GGGACCGGGA TCGAGACAGA GAACGAGACA GAGAGAGAGA TGGACCCTAC 
AGACGGTCAG ACTCGTATCC AGAGCGCAGA GGTGTGCGCA AAGGAAACAC AGTGTATGTG 
TACGGCACCG GTTTAGTGGA GGACAGCTTG CGCACAGCCT TTGCTCAGCA CGGCAACATC 
ATTGACCTTT CAATGGACTC TCCACGCAAT TGCGCTTTTG TCACCTTTGA GAAGATCGAA 
TCAGCAGACC AGGCTGTGGC GGAGCTGAAT GGCACCACTG TGGGTGACGT CAACATCAAA 
GTCAGCATTG CCAGGAAGCA GCCCATGCTG GATGCCGCCA CAGGGAAATC TGTCTGGGCT 
TCTCTTGCGG TGCAGAACAG TGCCAAGGGC TCGTACAGAG ACAAACGGAG CCAGGTCGTC 
TACAGTGAAG ATTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Nucleic acids research45(16)9547-9557(2017 Sep)
Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Blood128(13)1701-10(2016 09)
Yang Q,Liu X,Zhou T,Cook J,Nguyen K,Bai X

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP.
Theriogenology70(9)1525-35(2008 Dec)
Liu J,Sun YH,Wang YW,Wang N,Pei DS,Wang YP,Hu W,Zhu ZY

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NELFE cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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