rims2a cDNA ORF clone, Danio rerio(zebrafish)

The following rims2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rims2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149450 XM_021467013.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa149451 XM_021467014.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa21891 XM_021467015.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa55524 XM_005170744.3
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa55525 XM_017351550.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa55526 XM_009292619.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa55527 XM_009292618.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa149447 XM_021467011.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa149448 XM_017351550.2
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa149449 XM_021467012.1
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa149446 XM_009292619.3
Latest version!
Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149450
    Clone ID Related Accession (Same CDS sequence) XM_021467013.1
    Accession Version XM_021467013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAACA GGCAAATGGG AACGGCGGCT GGAGGCAACA TGACCAAGAG CACCAGCATC 
    GGCGGAGACA TGTGCAGCCT GGAGAAGACC GACGGCAGCC AGTCGGACAC GGCCGTAAGC
    CTGGTGGGAA CGGACGACAA GAAACGGCGC TCCAGCATCG GAGCTAAGAT GGCGGCAGTG
    GTGGGATTGA GCCGAAAGAG TCGCAGCGCT TCACAGCTCG GTCAGACAGA AGCAGGAGGG
    AAGAAGCTGC GCAGCACCAT CCAGAGGAGC ACAGAGACGG GACTGGCTGT GGAGATGAGG
    AGCCGAATGA CAAGACAAGC CAGCCGAGAA TCCACTGACG GCAGCATGAA CAGCTACAGC
    TCTGAGGGAA ACCTGATCTT CCCTGGAGTG AGACTCTCTT CAGACAGTCA GTTCAGTGAT
    TTCCTGGATG GACTTGGCCC AGCCCAGCTG GTGGGCAGAC AGACATTAGC CACACCTCCT
    ATGGGTGACA TTCAGATCGG GATGGTGGAA AAGAAAGGAG CCCTAGAGGT GGAGGTGATC
    CGAGCCCGAG GCCTTGTGGG AAAACCAGGT TCAAAGGCAC TGCCAGCCCC TTACGTAAAG
    GTGTATCTAC TGGAGAACGG CGCCTGCATA GCCAAAAAGA AAACAAAAGT AGCAAGAAAA
    ACACTGGATC CTCTTTACCA GCAGCAGCTG TCATTCGAAG AGTCTCCAGG AGGAAAAGTT
    TTACAGATTA TAGTGTGGGG AGATTATGGA CGTATGGACC ACAAATCTTT TATGGGAGCT
    GCTCAGATAC TGTTAGATGA TCTGGACTTG TCCAACATGG TTATTGGCTG GTTTAAGCTG
    TTTCCCCCAT CTTCACTGGT CGACCCAACC TTAGCCCCTT TGACCAGAAG AGCTTCCCAG
    ACATCTCTGG ACAGTGGGTC CGGGCCATTT GCTCGCTCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322688.1
    CDS140..1081
    Translation

    Target ORF information:

    RefSeq Version XM_021467013.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAACA GGCAAATGGG AACGGCGGCT GGAGGCAACA TGACCAAGAG CACCAGCATC 
    GGCGGAGACA TGTGCAGCCT GGAGAAGACC GACGGCAGCC AGTCGGACAC GGCCGTAAGC
    CTGGTGGGAA CGGACGACAA GAAACGGCGC TCCAGCATCG GAGCTAAGAT GGCGGCAGTG
    GTGGGATTGA GCCGAAAGAG TCGCAGCGCT TCACAGCTCG GTCAGACAGA AGCAGGAGGG
    AAGAAGCTGC GCAGCACCAT CCAGAGGAGC ACAGAGACGG GACTGGCTGT GGAGATGAGG
    AGCCGAATGA CAAGACAAGC CAGCCGAGAA TCCACTGACG GCAGCATGAA CAGCTACAGC
    TCTGAGGGAA ACCTGATCTT CCCTGGAGTG AGACTCTCTT CAGACAGTCA GTTCAGTGAT
    TTCCTGGATG GACTTGGCCC AGCCCAGCTG GTGGGCAGAC AGACATTAGC CACACCTCCT
    ATGGGTGACA TTCAGATCGG GATGGTGGAA AAGAAAGGAG CCCTAGAGGT GGAGGTGATC
    CGAGCCCGAG GCCTTGTGGG AAAACCAGGT TCAAAGGCAC TGCCAGCCCC TTACGTAAAG
    GTGTATCTAC TGGAGAACGG CGCCTGCATA GCCAAAAAGA AAACAAAAGT AGCAAGAAAA
    ACACTGGATC CTCTTTACCA GCAGCAGCTG TCATTCGAAG AGTCTCCAGG AGGAAAAGTT
    TTACAGATTA TAGTGTGGGG AGATTATGGA CGTATGGACC ACAAATCTTT TATGGGAGCT
    GCTCAGATAC TGTTAGATGA TCTGGACTTG TCCAACATGG TTATTGGCTG GTTTAAGCTG
    TTTCCCCCAT CTTCACTGGT CGACCCAACC TTAGCCCCTT TGACCAGAAG AGCTTCCCAG
    ACATCTCTGG ACAGTGGGTC CGGGCCATTT GCTCGCTCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149451
    Clone ID Related Accession (Same CDS sequence) XM_021467014.1
    Accession Version XM_021467014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGAACGG CGGCTGGAGG CAACATGACC AAGAGCACCA GCATCGGCGG AGACATGTGC 
    AGCCTGGAGA AGACCGACGG CAGCCAGTCG GACACGGCCG TAAGCCTGGT GGGAACGGAC
    GACAAGAAAC GGCGCTCCAG CATCGGAGCT AAGATGGCGG CAGTGGTGGG ATTGAGCCGA
    AAGAGTCGCA GCGCTTCACA GCTCGGTCAG ACAGAAGCAG GAGGGAAGAA GCTGCGCAGC
    ACCATCCAGA GGAGCACAGA GACGGGACTG GCTGTGGAGA TGAGGAGCCG AATGACAAGA
    CAAGCCAGCC GAGAATCCAC TGACGGCAGC ATGAACAGCT ACAGCTCTGA GGGAAACCTG
    ATCTTCCCTG GAGTGAGACT CTCTTCAGAC AGTCAGTTCA GTGATTTCCT GGATGGACTT
    GGCCCAGCCC AGCTGGTGGG CAGACAGACA TTAGCCACAC CTCCTATGGG TGACATTCAG
    ATCGGGATGG TGGAAAAGAA AGGAGCCCTA GAGGTGGAGG TGATCCGAGC CCGAGGCCTT
    GTGGGAAAAC CAGGTTCAAA GGCACTGCCA GCCCCTTACG TAAAGGTGTA TCTACTGGAG
    AACGGCGCCT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACACT GGATCCTCTT
    TACCAGCAGC AGCTGTCATT CGAAGAGTCT CCAGGAGGAA AAGTTTTACA GATTATAGTG
    TGGGGAGATT ATGGACGTAT GGACCACAAA TCTTTTATGG GAGCTGCTCA GATACTGTTA
    GATGATCTGG ACTTGTCCAA CATGGTTATT GGCTGGTTTA AGCTGTTTCC CCCATCTTCA
    CTGGTCGACC CAACCTTAGC CCCTTTGACC AGAAGAGCTT CCCAGACATC TCTGGACAGT
    GGGTCCGGGC CATTTGCTCG CTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322689.1
    CDS128..1054
    Translation

    Target ORF information:

    RefSeq Version XM_021467014.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGAACGG CGGCTGGAGG CAACATGACC AAGAGCACCA GCATCGGCGG AGACATGTGC 
    AGCCTGGAGA AGACCGACGG CAGCCAGTCG GACACGGCCG TAAGCCTGGT GGGAACGGAC
    GACAAGAAAC GGCGCTCCAG CATCGGAGCT AAGATGGCGG CAGTGGTGGG ATTGAGCCGA
    AAGAGTCGCA GCGCTTCACA GCTCGGTCAG ACAGAAGCAG GAGGGAAGAA GCTGCGCAGC
    ACCATCCAGA GGAGCACAGA GACGGGACTG GCTGTGGAGA TGAGGAGCCG AATGACAAGA
    CAAGCCAGCC GAGAATCCAC TGACGGCAGC ATGAACAGCT ACAGCTCTGA GGGAAACCTG
    ATCTTCCCTG GAGTGAGACT CTCTTCAGAC AGTCAGTTCA GTGATTTCCT GGATGGACTT
    GGCCCAGCCC AGCTGGTGGG CAGACAGACA TTAGCCACAC CTCCTATGGG TGACATTCAG
    ATCGGGATGG TGGAAAAGAA AGGAGCCCTA GAGGTGGAGG TGATCCGAGC CCGAGGCCTT
    GTGGGAAAAC CAGGTTCAAA GGCACTGCCA GCCCCTTACG TAAAGGTGTA TCTACTGGAG
    AACGGCGCCT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACACT GGATCCTCTT
    TACCAGCAGC AGCTGTCATT CGAAGAGTCT CCAGGAGGAA AAGTTTTACA GATTATAGTG
    TGGGGAGATT ATGGACGTAT GGACCACAAA TCTTTTATGG GAGCTGCTCA GATACTGTTA
    GATGATCTGG ACTTGTCCAA CATGGTTATT GGCTGGTTTA AGCTGTTTCC CCCATCTTCA
    CTGGTCGACC CAACCTTAGC CCCTTTGACC AGAAGAGCTT CCCAGACATC TCTGGACAGT
    GGGTCCGGGC CATTTGCTCG CTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21891
    Clone ID Related Accession (Same CDS sequence) XM_021467015.1
    Accession Version XM_021467015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGTAGAC AGAGCCATGA TGCCACCTCG ACAGCGGGCA GCGGCAGTCG CATGCAGCGC 
    TCCCAGAGCC GAATGAGTCT GTCAGCCTCT TTCGAAGCTC TAGCTGTTTA TTTCCCATGC
    ATGAATTCTT TCGATGAGGA GGATGGAGAA GCAGGAGGGA AGAAGCTGCG CAGCACCATC
    CAGAGGAGCA CAGAGACGGG ACTGGCTGTG GAGATGAGGA GCCGAATGAC AAGACAAGCC
    AGCCGAGAAT CCACTGACGG CAGCATGAAC AGCTACAGCT CTGAGGGAAA CCTGATCTTC
    CCTGGAGTGA GACTCTCTTC AGACAGTCAG TTCAGTGATT TCCTGGATGG ACTTGGCCCA
    GCCCAGCTGG TGGGCAGACA GACATTAGCC ACACCTCCTA TGGGTGACAT TCAGATCGGG
    ATGGTGGAAA AGAAAGGAGC CCTAGAGGTG GAGGTGATCC GAGCCCGAGG CCTTGTGGGA
    AAACCAGGTT CAAAGGCACT GCCAGCCCCT TACGTAAAGG TGTATCTACT GGAGAACGGC
    GCCTGCATAG CCAAAAAGAA AACAAAAGTA GCAAGAAAAA CACTGGATCC TCTTTACCAG
    CAGCAGCTGT CATTCGAAGA GTCTCCAGGA GGAAAAGTTT TACAGATTAT AGTGTGGGGA
    GATTATGGAC GTATGGACCA CAAATCTTTT ATGGGAGCTG CTCAGATACT GTTAGATGAT
    CTGGACTTGT CCAACATGGT TATTGGCTGG TTTAAGCTGT TTCCCCCATC TTCACTGGTC
    GACCCAACCT TAGCCCCTTT GACCAGAAGA GCTTCCCAGA CATCTCTGGA CAGTGGGTCC
    GGGCCATTTG CTCGCTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322690.1
    CDS177..1037
    Translation

    Target ORF information:

    RefSeq Version XM_021467015.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGTAGAC AGAGCCATGA TGCCACCTCG ACAGCGGGCA GCGGCAGTCG CATGCAGCGC 
    TCCCAGAGCC GAATGAGTCT GTCAGCCTCT TTCGAAGCTC TAGCTGTTTA TTTCCCATGC
    ATGAATTCTT TCGATGAGGA GGATGGAGAA GCAGGAGGGA AGAAGCTGCG CAGCACCATC
    CAGAGGAGCA CAGAGACGGG ACTGGCTGTG GAGATGAGGA GCCGAATGAC AAGACAAGCC
    AGCCGAGAAT CCACTGACGG CAGCATGAAC AGCTACAGCT CTGAGGGAAA CCTGATCTTC
    CCTGGAGTGA GACTCTCTTC AGACAGTCAG TTCAGTGATT TCCTGGATGG ACTTGGCCCA
    GCCCAGCTGG TGGGCAGACA GACATTAGCC ACACCTCCTA TGGGTGACAT TCAGATCGGG
    ATGGTGGAAA AGAAAGGAGC CCTAGAGGTG GAGGTGATCC GAGCCCGAGG CCTTGTGGGA
    AAACCAGGTT CAAAGGCACT GCCAGCCCCT TACGTAAAGG TGTATCTACT GGAGAACGGC
    GCCTGCATAG CCAAAAAGAA AACAAAAGTA GCAAGAAAAA CACTGGATCC TCTTTACCAG
    CAGCAGCTGT CATTCGAAGA GTCTCCAGGA GGAAAAGTTT TACAGATTAT AGTGTGGGGA
    GATTATGGAC GTATGGACCA CAAATCTTTT ATGGGAGCTG CTCAGATACT GTTAGATGAT
    CTGGACTTGT CCAACATGGT TATTGGCTGG TTTAAGCTGT TTCCCCCATC TTCACTGGTC
    GACCCAACCT TAGCCCCTTT GACCAGAAGA GCTTCCCAGA CATCTCTGGA CAGTGGGTCC
    GGGCCATTTG CTCGCTCGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55524
    Clone ID Related Accession (Same CDS sequence) XM_005170744.3
    Accession Version XM_005170744.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) and transcript sequence (GDQH01043789.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170744.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 101 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GACCCAAGGC
    CCGTTTGTGT ACAGCTCAGG GTCTCAGAGG ATCAGCGACA GCGAGTTCTC AGACTATGAC
    TGTGAGGATG GCGTTGGCGT CGTATCAGAC TATAGAAACG GCAGGGACCT CCAGAGCTCC
    ACCCTCTCTG TGCCTGAGCA AGTCTTATCG TCCAATCACT GCTCTCGGTC TGCTGATATC
    AACAGAGCGC GCTCGCGATC TCCCAGCATG CCTCCTTCTC AGAGTCATTT TCTCACGTTA
    CCTCGAACCC GACACAGTCA GCCTCATGAC AGTGACCTGC AGGATCTCAG GAATTACGGA
    GGAGCCCCGG ACCGGCACGA GTACCACCAT CGATCTCGTT CTGCAGACCA ACGGGCCGCA
    ATGGAGCGTC CCATGTACAG TCGCTCTCGC TCCACAGAGC GTCCGCCCGA CGGACCGCAC
    ATGAGGTCCT CCATGCCCTC TCTGCCATCG GGACACTCTG CACCATCTTC CCCTGCCTTA
    TCGAGGGCTC ATCCTCGCGG TGGATCGGTT CAGACCAGCC CCACAGGGAC CCCCATAAAC
    AGCCGGAGAG GAAGACAGCT GCCACAACTG CCTCCAAAGG GGACGCTGGA GAGAAAAGAT
    GGAGAAGAAG AAGTTGAGGC TTGCCCAGAC TCGGACTCCC CCAGTCGGAC AAACTCAGGT
    GCTCTGGATG TTGAGGAGCG AACGCGGCAG ATGAAGCTGA AGATGAACAA ATATAAACAG
    GGGACCGGCT CTGATTCAAG ACTGGAGCAG GACTATCACA AGCGCTCCGG CAGGGATCCT
    CAGCGTGGAG AAAACCTGTC GGCCAAGTCT TCGGACAGTG ATGTGAGCGA CGTGTCCGCT
    GTGTCGAGAA CCAGCAGTGC ATCTCGATTC AGCAGCATGA GCTACATCTC TGTCCAGTCG
    GAGCGGCCAC GGGGCAGCAG GAAGATCAGC GGGCGGAGTC TCAGCAGGGA GGGGGAGGAG
    CTTCAGGAGG GGGAGGGTCT TCGGGAGGAG GTGGGGGGGT TAGAGGAGGA AAGGGGAAGC
    AGAAGGAAAA CAGGGAAACT CAGCAGACAG ACAGAAAGTG TAGAGCTGGA GGAGTCTGAG
    GAGGACAAGA ACCAGAGGAC GCTCTCTGAG ACCGACCTCA GGCAAATGGG AACGGCGGCT
    GGAGGCAACA TGACCAAGAG CACCAGCATC GGCGGAGACA TGTGCAGCCT GGAGAAGACC
    GACGGCAGCC AGTCGGACAC GGCCGTAAGC CTGGTGGGAA CGGACGACAA GAAACGGCGC
    TCCAGCATCG GAGCTAAGAT GGCGGCAGTG GTGGGATTGA GCCGAAAGAG TCGCAGCGCT
    TCACAGCTCG GTCAGACAGA AGCAGGAGGG AAGAAGCTGC GCAGCACCAT CCAGAGGAGC
    ACAGAGACGG GACTGGCTGT GGAGATGAGG AGCCGAATGA CAAGACAAGC CAGCCGAGAA
    TCCACTGACG GCAGCATGAA CAGCTACAGC TCTGAGGGAA ACCTGATCTT CCCTGGAGTG
    AGACTCTCTT CAGACAGTCA GTTCAGTGAT TTCCTGGATG GACTTGGCCC AGCCCAGCTG
    GTGGGCAGAC AGACATTAGC CACACCTCCT ATGGGTGACA TTCAGATCGG GATGGTGGAA
    AAGAAAGGAG CCCTAGAGGT GGAGGTGATC CGAGCCCGAG GCCTTGTGGG AAAACCAGGT
    TCAAAGGCAC TGCCAGCCCC TTACGTAAAG GTGTATCTAC TGGAGAACGG CGCCTGCATA
    GCCAAAAAGA AAACAAAAGT AGCAAGAAAA ACACTGGATC CTCTTTACCA GCAGCAGCTG
    TCATTCGAAG AGTCTCCAGG AGGAAAAGTT TTACAGATTA TAGTGTGGGG AGATTATGGA
    CGTATGGACC ACAAATCTTT TATGGGAGCT GCTCAGATAC TGTTAGATGA TCTGGACTTG
    TCCAACATGG TTATTGGCTG GTTTAAGCTG TTTCCCCCAT CTTCACTGGT CGACCCAACC
    TTAGCCCCTT TGACCAGAAG AGCTTCCCAG ACATCTCTGG ACAGTGGGTC CGGGCCATTT
    GCTCGCTCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170801.2
    CDS15..2666
    Translation

    Target ORF information:

    RefSeq Version XM_005170744.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170744.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GACCCAAGGC
    CCGTTTGTGT ACAGCTCAGG GTCTCAGAGG ATCAGCGACA GCGAGTTCTC AGACTATGAC
    TGTGAGGATG GCGTTGGCGT CGTATCAGAC TATAGAAACG GCAGGGACCT CCAGAGCTCC
    ACCCTCTCTG TGCCTGAGCA AGTCTTATCG TCCAATCACT GCTCTCGGTC TGCTGATATC
    AACAGAGCGC GCTCGCGATC TCCCAGCATG CCTCCTTCTC AGAGTCATTT TCTCACGTTA
    CCTCGAACCC GACACAGTCA GCCTCATGAC AGTGACCTGC AGGATCTCAG GAATTACGGA
    GGAGCCCCGG ACCGGCACGA GTACCACCAT CGATCTCGTT CTGCAGACCA ACGGGCCGCA
    ATGGAGCGTC CCATGTACAG TCGCTCTCGC TCCACAGAGC GTCCGCCCGA CGGACCGCAC
    ATGAGGTCCT CCATGCCCTC TCTGCCATCG GGACACTCTG CACCATCTTC CCCTGCCTTA
    TCGAGGGCTC ATCCTCGCGG TGGATCGGTT CAGACCAGCC CCACAGGGAC CCCCATAAAC
    AGCCGGAGAG GAAGACAGCT GCCACAACTG CCTCCAAAGG GGACGCTGGA GAGAAAAGAT
    GGAGAAGAAG AAGTTGAGGC TTGCCCAGAC TCGGACTCCC CCAGTCGGAC AAACTCAGGT
    GCTCTGGATG TTGAGGAGCG AACGCGGCAG ATGAAGCTGA AGATGAACAA ATATAAACAG
    GGGACCGGCT CTGATTCAAG ACTGGAGCAG GACTATCACA AGCGCTCCGG CAGGGATCCT
    CAGCGTGGAG AAAACCTGTC GGCCAAGTCT TCGGACAGTG ATGTGAGCGA CGTGTCCGCT
    GTGTCGAGAA CCAGCAGTGC ATCTCGATTC AGCAGCATGA GCTACATCTC TGTCCAGTCG
    GAGCGGCCAC GGGGCAGCAG GAAGATCAGC GGGCGGAGTC TCAGCAGGGA GGGGGAGGAG
    CTTCAGGAGG GGGAGGGTCT TCGGGAGGAG GTGGGGGGGT TAGAGGAGGA AAGGGGAAGC
    AGAAGGAAAA CAGGGAAACT CAGCAGACAG ACAGAAAGTG TAGAGCTGGA GGAGTCTGAG
    GAGGACAAGA ACCAGAGGAC GCTCTCTGAG ACCGACCTCA GGCAAATGGG AACGGCGGCT
    GGAGGCAACA TGACCAAGAG CACCAGCATC GGCGGAGACA TGTGCAGCCT GGAGAAGACC
    GACGGCAGCC AGTCGGACAC GGCCGTAAGC CTGGTGGGAA CGGACGACAA GAAACGGCGC
    TCCAGCATCG GAGCTAAGAT GGCGGCAGTG GTGGGATTGA GCCGAAAGAG TCGCAGCGCT
    TCACAGCTCG GTCAGACAGA AGCAGGAGGG AAGAAGCTGC GCAGCACCAT CCAGAGGAGC
    ACAGAGACGG GACTGGCTGT GGAGATGAGG AGCCGAATGA CAAGACAAGC CAGCCGAGAA
    TCCACTGACG GCAGCATGAA CAGCTACAGC TCTGAGGGAA ACCTGATCTT CCCTGGAGTG
    AGACTCTCTT CAGACAGTCA GTTCAGTGAT TTCCTGGATG GACTTGGCCC AGCCCAGCTG
    GTGGGCAGAC AGACATTAGC CACACCTCCT ATGGGTGACA TTCAGATCGG GATGGTGGAA
    AAGAAAGGAG CCCTAGAGGT GGAGGTGATC CGAGCCCGAG GCCTTGTGGG AAAACCAGGT
    TCAAAGGCAC TGCCAGCCCC TTACGTAAAG GTGTATCTAC TGGAGAACGG CGCCTGCATA
    GCCAAAAAGA AAACAAAAGT AGCAAGAAAA ACACTGGATC CTCTTTACCA GCAGCAGCTG
    TCATTCGAAG AGTCTCCAGG AGGAAAAGTT TTACAGATTA TAGTGTGGGG AGATTATGGA
    CGTATGGACC ACAAATCTTT TATGGGAGCT GCTCAGATAC TGTTAGATGA TCTGGACTTG
    TCCAACATGG TTATTGGCTG GTTTAAGCTG TTTCCCCCAT CTTCACTGGT CGACCCAACC
    TTAGCCCCTT TGACCAGAAG AGCTTCCCAG ACATCTCTGG ACAGTGGGTC CGGGCCATTT
    GCTCGCTCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55525
    Clone ID Related Accession (Same CDS sequence) XM_017351550.1
    Accession Version XM_017351550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) and transcript sequence (GDQH01043789.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 101 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GACCCAAGGC
    CCGTTTGTGT ACAGCTCAGG GTCTCAGAGG ATCAGCGACA GCGAGTTCTC AGACTATGAC
    TGTGAGGATG GCGTTGGCGT CGTATCAGAC TATAGAAACG GCAGGGACCT CCAGAGCTCC
    ACCCTCTCTG TGCCTGAGCA AGTCTTATCG TCCAATCACT GCTCTCGGTC TGCTGATATC
    AACAGAGCGC GCTCGCGATC TCCCAGCATG CCTCCTTCTC AGAGTCATTT TCTCACGTTA
    CCTCGAACCC GACACAGTCA GCCTCATGAC AGTGACCTGC AGGATCTCAG GAATTACGGA
    GGAGCCCCGG ACCGGCACGA GTACCACCAT CGATCTCGTT CTGCAGACCA ACGGGCCGCA
    ATGGAGCGTC CCATGTACAG TCGCTCTCGC TCCACAGAGC GTCCGCCCGA CGGACCGCAC
    ATGAGGTCCT CCATGCCCTC TCTGCCATCG GGACACTCTG CACCATCTTC CCCTGCCTTA
    TCGAGGGCTC ATCCTCGCGG TGGATCGGTT CAGACCAGCC CCACAGGGAC CCCCATAAAC
    AGCCGGAGAG GAAGACAGCT GCCACAACTG CCTCCAAAGG GGACGCTGGA GAGAAAAGAT
    GGAGAAGAAG AAGTTGAGGC TTGCCCAGAC TCGGACTCCC CCAGTCGGAC AAACTCAGGT
    GCTCTGGATG TTGAGGAGCG AACGCGGCAG ATGAAGCTGA AGATGAACAA ATATAAACAG
    GGGACCGGCT CTGATTCAAG ACTGGAGCAG GACTATCACA AGCGCTCCGG CAGGGATCCT
    CAGCGTGGAG AAAACCTGTC GGCCAAGTCT TCGGACAGTG ATGTGAGCGA CGTGTCCGCT
    GTGTCGAGAA CCAGCAGTGC ATCTCGATTC AGCAGCATGA GCTACATCTC TGTCCAGTCG
    GAGCGGCCAC GGGGCAGCAG GAAGATCAGT GAATTTACAT CCAAAATGAA AAACAGGCAA
    ATGGGAACGG CGGCTGGAGG CAACATGACC AAGAGCACCA GCATCGGCGG AGACATGTGC
    AGCCTGGAGA AGACCGACGG CAGCCAGTCG GACACGGCCG TAAGCCTGGT GGGAACGGAC
    GACAAGAAAC GGCGCTCCAG CATCGGAGCT AAGATGGCGG CAGTGGTGGG ATTGAGCCGA
    AAGAGTCGCA GCGCTTCACA GCTCGGTCAG ACAGAAGCAG GAGGGAAGAA GCTGCGCAGC
    ACCATCCAGA GGAGCACAGA GACGGGACTG GCTGTGGAGA TGAGGAGCCG AATGACAAGA
    CAAGCCAGCC GAGAATCCAC TGACGGCAGC ATGAACAGCT ACAGCTCTGA GGGAAACCTG
    ATCTTCCCTG GAGTGAGACT CTCTTCAGAC AGTCAGTTCA GTGATTTCCT GGATGGACTT
    GGCCCAGCCC AGCTGGTGGG CAGACAGACA TTAGCCACAC CTCCTATGGG TGACATTCAG
    ATCGGGATGG TGGAAAAGAA AGGAGCCCTA GAGGTGGAGG TGATCCGAGC CCGAGGCCTT
    GTGGGAAAAC CAGGTTCAAA GGCACTGCCA GCCCCTTACG TAAAGGTGTA TCTACTGGAG
    AACGGCGCCT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACACT GGATCCTCTT
    TACCAGCAGC AGCTGTCATT CGAAGAGTCT CCAGGAGGAA AAGTTTTACA GATTATAGTG
    TGGGGAGATT ATGGACGTAT GGACCACAAA TCTTTTATGG GAGCTGCTCA GATACTGTTA
    GATGATCTGG ACTTGTCCAA CATGGTTATT GGCTGGTTTA AGCTGTTTCC CCCATCTTCA
    CTGGTCGACC CAACCTTAGC CCCTTTGACC AGAAGAGCTT CCCAGACATC TCTGGACAGT
    GGGTCCGGGC CATTTGCTCG CTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207039.1
    CDS15..2501
    Translation

    Target ORF information:

    RefSeq Version XM_017351550.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GACCCAAGGC
    CCGTTTGTGT ACAGCTCAGG GTCTCAGAGG ATCAGCGACA GCGAGTTCTC AGACTATGAC
    TGTGAGGATG GCGTTGGCGT CGTATCAGAC TATAGAAACG GCAGGGACCT CCAGAGCTCC
    ACCCTCTCTG TGCCTGAGCA AGTCTTATCG TCCAATCACT GCTCTCGGTC TGCTGATATC
    AACAGAGCGC GCTCGCGATC TCCCAGCATG CCTCCTTCTC AGAGTCATTT TCTCACGTTA
    CCTCGAACCC GACACAGTCA GCCTCATGAC AGTGACCTGC AGGATCTCAG GAATTACGGA
    GGAGCCCCGG ACCGGCACGA GTACCACCAT CGATCTCGTT CTGCAGACCA ACGGGCCGCA
    ATGGAGCGTC CCATGTACAG TCGCTCTCGC TCCACAGAGC GTCCGCCCGA CGGACCGCAC
    ATGAGGTCCT CCATGCCCTC TCTGCCATCG GGACACTCTG CACCATCTTC CCCTGCCTTA
    TCGAGGGCTC ATCCTCGCGG TGGATCGGTT CAGACCAGCC CCACAGGGAC CCCCATAAAC
    AGCCGGAGAG GAAGACAGCT GCCACAACTG CCTCCAAAGG GGACGCTGGA GAGAAAAGAT
    GGAGAAGAAG AAGTTGAGGC TTGCCCAGAC TCGGACTCCC CCAGTCGGAC AAACTCAGGT
    GCTCTGGATG TTGAGGAGCG AACGCGGCAG ATGAAGCTGA AGATGAACAA ATATAAACAG
    GGGACCGGCT CTGATTCAAG ACTGGAGCAG GACTATCACA AGCGCTCCGG CAGGGATCCT
    CAGCGTGGAG AAAACCTGTC GGCCAAGTCT TCGGACAGTG ATGTGAGCGA CGTGTCCGCT
    GTGTCGAGAA CCAGCAGTGC ATCTCGATTC AGCAGCATGA GCTACATCTC TGTCCAGTCG
    GAGCGGCCAC GGGGCAGCAG GAAGATCAGT GAATTTACAT CCAAAATGAA AAACAGGCAA
    ATGGGAACGG CGGCTGGAGG CAACATGACC AAGAGCACCA GCATCGGCGG AGACATGTGC
    AGCCTGGAGA AGACCGACGG CAGCCAGTCG GACACGGCCG TAAGCCTGGT GGGAACGGAC
    GACAAGAAAC GGCGCTCCAG CATCGGAGCT AAGATGGCGG CAGTGGTGGG ATTGAGCCGA
    AAGAGTCGCA GCGCTTCACA GCTCGGTCAG ACAGAAGCAG GAGGGAAGAA GCTGCGCAGC
    ACCATCCAGA GGAGCACAGA GACGGGACTG GCTGTGGAGA TGAGGAGCCG AATGACAAGA
    CAAGCCAGCC GAGAATCCAC TGACGGCAGC ATGAACAGCT ACAGCTCTGA GGGAAACCTG
    ATCTTCCCTG GAGTGAGACT CTCTTCAGAC AGTCAGTTCA GTGATTTCCT GGATGGACTT
    GGCCCAGCCC AGCTGGTGGG CAGACAGACA TTAGCCACAC CTCCTATGGG TGACATTCAG
    ATCGGGATGG TGGAAAAGAA AGGAGCCCTA GAGGTGGAGG TGATCCGAGC CCGAGGCCTT
    GTGGGAAAAC CAGGTTCAAA GGCACTGCCA GCCCCTTACG TAAAGGTGTA TCTACTGGAG
    AACGGCGCCT GCATAGCCAA AAAGAAAACA AAAGTAGCAA GAAAAACACT GGATCCTCTT
    TACCAGCAGC AGCTGTCATT CGAAGAGTCT CCAGGAGGAA AAGTTTTACA GATTATAGTG
    TGGGGAGATT ATGGACGTAT GGACCACAAA TCTTTTATGG GAGCTGCTCA GATACTGTTA
    GATGATCTGG ACTTGTCCAA CATGGTTATT GGCTGGTTTA AGCTGTTTCC CCCATCTTCA
    CTGGTCGACC CAACCTTAGC CCCTTTGACC AGAAGAGCTT CCCAGACATC TCTGGACAGT
    GGGTCCGGGC CATTTGCTCG CTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55526
    Clone ID Related Accession (Same CDS sequence) XM_009292619.2
    Accession Version XM_009292619.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2460bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) and transcript sequence (GDQH01043789.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 101 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GACCCAAGGC
    CCGTTTGTGT ACAGCTCAGG GTCTCAGAGG ATCAGCGACA GCGAGTTCTC AGACTATGAC
    TGTGAGGATG GCGTTGGCGT CGTATCAGAC TATAGAAACG GCAGGGACCT CCAGAGCTCC
    ACCCTCTCTG TGCCTGAGCA AGTCTTATCG TCCAATCACT GCTCTCGGTC TGCTGATATC
    AACAGAGCGC GCTCGCGATC TCCCAGCATG CCTCCTTCTC AGAGTCATTT TCTCACGTTA
    CCTCGAACCC GACACAGTCA GCCTCATGAC AGTGACCTGC AGGATCTCAG GAATTACGGA
    GGAGCCCCGG ACCGGCACGA GTACCACCAT CGATCTCGTT CTGCAGACCA ACGGGCCGCA
    ATGGAGCGTC CCATGTACAG TCGCTCTCGC TCCACAGAGC GTCCGCCCGA CGGACCGCAC
    ATGAGGTCCT CCATGCCCTC TCTGCCATCG GGACACTCTG CACCATCTTC CCCTGCCTTA
    TCGAGGGCTC ATCCTCGCGG TGGATCGGTT CAGACCAGCC CCACAGGGAC CCCCATAAAC
    AGCCGGAGAG GAAGACAGCT GCCACAACTG CCTCCAAAGG GGACGCTGGA GAGAAAAGAT
    GGAGAAGAAG AAGTTGAGGC TTGCCCAGAC TCGGACTCCC CCAGTCGGAC AAACTCAGGT
    GCTCTGGATG TTGAGGAGCG AACGCGGCAG ATGAAGCTGA AGATGAACAA ATATAAACAG
    GGGACCGGCT CTGATTCAAG ACTGGAGCAG GACTATCACA AGCGCTCCGG CAGGGATCCT
    CAGCGTGGAG AAAACCTGTC GGCCAAGTCT TCGGACAGTG ATGTGAGCGA CGTGTCCGCT
    GTGTCGAGAA CCAGCAGTGC ATCTCGATTC AGCAGCATGA GCTACATCTC TGTCCAGTCG
    GAGCGGCCAC GGGGCAGCAG GAAGATCAGG CAAATGGGAA CGGCGGCTGG AGGCAACATG
    ACCAAGAGCA CCAGCATCGG CGGAGACATG TGCAGCCTGG AGAAGACCGA CGGCAGCCAG
    TCGGACACGG CCGTAAGCCT GGTGGGAACG GACGACAAGA AACGGCGCTC CAGCATCGGA
    GCTAAGATGG CGGCAGTGGT GGGATTGAGC CGAAAGAGTC GCAGCGCTTC ACAGCTCGGT
    CAGACAGAAG CAGGAGGGAA GAAGCTGCGC AGCACCATCC AGAGGAGCAC AGAGACGGGA
    CTGGCTGTGG AGATGAGGAG CCGAATGACA AGACAAGCCA GCCGAGAATC CACTGACGGC
    AGCATGAACA GCTACAGCTC TGAGGGAAAC CTGATCTTCC CTGGAGTGAG ACTCTCTTCA
    GACAGTCAGT TCAGTGATTT CCTGGATGGA CTTGGCCCAG CCCAGCTGGT GGGCAGACAG
    ACATTAGCCA CACCTCCTAT GGGTGACATT CAGATCGGGA TGGTGGAAAA GAAAGGAGCC
    CTAGAGGTGG AGGTGATCCG AGCCCGAGGC CTTGTGGGAA AACCAGGTTC AAAGGCACTG
    CCAGCCCCTT ACGTAAAGGT GTATCTACTG GAGAACGGCG CCTGCATAGC CAAAAAGAAA
    ACAAAAGTAG CAAGAAAAAC ACTGGATCCT CTTTACCAGC AGCAGCTGTC ATTCGAAGAG
    TCTCCAGGAG GAAAAGTTTT ACAGATTATA GTGTGGGGAG ATTATGGACG TATGGACCAC
    AAATCTTTTA TGGGAGCTGC TCAGATACTG TTAGATGATC TGGACTTGTC CAACATGGTT
    ATTGGCTGGT TTAAGCTGTT TCCCCCATCT TCACTGGTCG ACCCAACCTT AGCCCCTTTG
    ACCAGAAGAG CTTCCCAGAC ATCTCTGGAC AGTGGGTCCG GGCCATTTGC TCGCTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290894.2
    CDS15..2474
    Translation

    Target ORF information:

    RefSeq Version XM_009292619.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292619.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GACCCAAGGC
    CCGTTTGTGT ACAGCTCAGG GTCTCAGAGG ATCAGCGACA GCGAGTTCTC AGACTATGAC
    TGTGAGGATG GCGTTGGCGT CGTATCAGAC TATAGAAACG GCAGGGACCT CCAGAGCTCC
    ACCCTCTCTG TGCCTGAGCA AGTCTTATCG TCCAATCACT GCTCTCGGTC TGCTGATATC
    AACAGAGCGC GCTCGCGATC TCCCAGCATG CCTCCTTCTC AGAGTCATTT TCTCACGTTA
    CCTCGAACCC GACACAGTCA GCCTCATGAC AGTGACCTGC AGGATCTCAG GAATTACGGA
    GGAGCCCCGG ACCGGCACGA GTACCACCAT CGATCTCGTT CTGCAGACCA ACGGGCCGCA
    ATGGAGCGTC CCATGTACAG TCGCTCTCGC TCCACAGAGC GTCCGCCCGA CGGACCGCAC
    ATGAGGTCCT CCATGCCCTC TCTGCCATCG GGACACTCTG CACCATCTTC CCCTGCCTTA
    TCGAGGGCTC ATCCTCGCGG TGGATCGGTT CAGACCAGCC CCACAGGGAC CCCCATAAAC
    AGCCGGAGAG GAAGACAGCT GCCACAACTG CCTCCAAAGG GGACGCTGGA GAGAAAAGAT
    GGAGAAGAAG AAGTTGAGGC TTGCCCAGAC TCGGACTCCC CCAGTCGGAC AAACTCAGGT
    GCTCTGGATG TTGAGGAGCG AACGCGGCAG ATGAAGCTGA AGATGAACAA ATATAAACAG
    GGGACCGGCT CTGATTCAAG ACTGGAGCAG GACTATCACA AGCGCTCCGG CAGGGATCCT
    CAGCGTGGAG AAAACCTGTC GGCCAAGTCT TCGGACAGTG ATGTGAGCGA CGTGTCCGCT
    GTGTCGAGAA CCAGCAGTGC ATCTCGATTC AGCAGCATGA GCTACATCTC TGTCCAGTCG
    GAGCGGCCAC GGGGCAGCAG GAAGATCAGG CAAATGGGAA CGGCGGCTGG AGGCAACATG
    ACCAAGAGCA CCAGCATCGG CGGAGACATG TGCAGCCTGG AGAAGACCGA CGGCAGCCAG
    TCGGACACGG CCGTAAGCCT GGTGGGAACG GACGACAAGA AACGGCGCTC CAGCATCGGA
    GCTAAGATGG CGGCAGTGGT GGGATTGAGC CGAAAGAGTC GCAGCGCTTC ACAGCTCGGT
    CAGACAGAAG CAGGAGGGAA GAAGCTGCGC AGCACCATCC AGAGGAGCAC AGAGACGGGA
    CTGGCTGTGG AGATGAGGAG CCGAATGACA AGACAAGCCA GCCGAGAATC CACTGACGGC
    AGCATGAACA GCTACAGCTC TGAGGGAAAC CTGATCTTCC CTGGAGTGAG ACTCTCTTCA
    GACAGTCAGT TCAGTGATTT CCTGGATGGA CTTGGCCCAG CCCAGCTGGT GGGCAGACAG
    ACATTAGCCA CACCTCCTAT GGGTGACATT CAGATCGGGA TGGTGGAAAA GAAAGGAGCC
    CTAGAGGTGG AGGTGATCCG AGCCCGAGGC CTTGTGGGAA AACCAGGTTC AAAGGCACTG
    CCAGCCCCTT ACGTAAAGGT GTATCTACTG GAGAACGGCG CCTGCATAGC CAAAAAGAAA
    ACAAAAGTAG CAAGAAAAAC ACTGGATCCT CTTTACCAGC AGCAGCTGTC ATTCGAAGAG
    TCTCCAGGAG GAAAAGTTTT ACAGATTATA GTGTGGGGAG ATTATGGACG TATGGACCAC
    AAATCTTTTA TGGGAGCTGC TCAGATACTG TTAGATGATC TGGACTTGTC CAACATGGTT
    ATTGGCTGGT TTAAGCTGTT TCCCCCATCT TCACTGGTCG ACCCAACCTT AGCCCCTTTG
    ACCAGAAGAG CTTCCCAGAC ATCTCTGGAC AGTGGGTCCG GGCCATTTGC TCGCTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55527
    Clone ID Related Accession (Same CDS sequence) XM_009292618.2
    Accession Version XM_009292618.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2460bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) and transcript sequence (GDQH01043789.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 101 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GAGGTCTCAG
    AGGATCAGCG ACAGCGAGTT CTCAGACTAT GACTGTGAGG ATGGCGTTGG CGTCGTATCA
    GACTATAGAA ACGGCAGGGA CCTCCAGAGC TCCACCCTCT CTGTGCCTGA GCAAGTCTTA
    TCGTCCAATC ACTGCTCTCG GTCTGCTGAT ATCAACAGAG CGCGCTCGCG ATCTCCCAGC
    ATGCCTCCTT CTCAGAGTCA TTTTCTCACG TTACCTCGAA CCCGACACAG TCAGCCTCAT
    GACAGTGACC TGCAGGATCT CAGGAATTAC GGAGGAGCCC CGGACCGGCA CGAGTACCAC
    CATCGATCTC GTTCTGCAGA CCAACGGGCC GCAATGGAGC GTCCCATGTA CAGTCGCTCT
    CGCTCCACAG AGCGTCCGCC CGACGGACCG CACATGAGGT CCTCCATGCC CTCTCTGCCA
    TCGGGACACT CTGCACCATC TTCCCCTGCC TTATCGAGGG CTCATCCTCG CGGTGGATCG
    GTTCAGACCA GCCCCACAGG GACCCCCATA AACAGCCGGA GAGGAAGACA GCTGCCACAA
    CTGCCTCCAA AGGGGACGCT GGAGAGAAAA GATGGAGAAG AAGAAGTTGA GGCTTGCCCA
    GACTCGGACT CCCCCAGTCG GACAAACTCA GGTGCTCTGG ATGTTGAGGA GCGAACGCGG
    CAGATGAAGC TGAAGATGAA CAAATATAAA CAGGGGACCG GCTCTGATTC AAGACTGGAG
    CAGGACTATC ACAAGCGCTC CGGCAGGGAT CCTCAGCGTG GAGAAAACCT GTCGGCCAAG
    TCTTCGGACA GTGATGTGAG CGACGTGTCC GCTGTGTCGA GAACCAGCAG TGCATCTCGA
    TTCAGCAGCA TGAGCTACAT CTCTGTCCAG TCGGAGCGGC CACGGGGCAG CAGGAAGATC
    AGTGAATTTA CATCCAAAAT GAAAAACAGG CAAATGGGAA CGGCGGCTGG AGGCAACATG
    ACCAAGAGCA CCAGCATCGG CGGAGACATG TGCAGCCTGG AGAAGACCGA CGGCAGCCAG
    TCGGACACGG CCGTAAGCCT GGTGGGAACG GACGACAAGA AACGGCGCTC CAGCATCGGA
    GCTAAGATGG CGGCAGTGGT GGGATTGAGC CGAAAGAGTC GCAGCGCTTC ACAGCTCGGT
    CAGACAGAAG CAGGAGGGAA GAAGCTGCGC AGCACCATCC AGAGGAGCAC AGAGACGGGA
    CTGGCTGTGG AGATGAGGAG CCGAATGACA AGACAAGCCA GCCGAGAATC CACTGACGGC
    AGCATGAACA GCTACAGCTC TGAGGGAAAC CTGATCTTCC CTGGAGTGAG ACTCTCTTCA
    GACAGTCAGT TCAGTGATTT CCTGGATGGA CTTGGCCCAG CCCAGCTGGT GGGCAGACAG
    ACATTAGCCA CACCTCCTAT GGGTGACATT CAGATCGGGA TGGTGGAAAA GAAAGGAGCC
    CTAGAGGTGG AGGTGATCCG AGCCCGAGGC CTTGTGGGAA AACCAGGTTC AAAGGCACTG
    CCAGCCCCTT ACGTAAAGGT GTATCTACTG GAGAACGGCG CCTGCATAGC CAAAAAGAAA
    ACAAAAGTAG CAAGAAAAAC ACTGGATCCT CTTTACCAGC AGCAGCTGTC ATTCGAAGAG
    TCTCCAGGAG GAAAAGTTTT ACAGATTATA GTGTGGGGAG ATTATGGACG TATGGACCAC
    AAATCTTTTA TGGGAGCTGC TCAGATACTG TTAGATGATC TGGACTTGTC CAACATGGTT
    ATTGGCTGGT TTAAGCTGTT TCCCCCATCT TCACTGGTCG ACCCAACCTT AGCCCCTTTG
    ACCAGAAGAG CTTCCCAGAC ATCTCTGGAC AGTGGGTCCG GGCCATTTGC TCGCTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290893.2
    CDS15..2474
    Translation

    Target ORF information:

    RefSeq Version XM_009292618.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGCGTC CCTCCATATC CGTCACGTCT CCCATGAGCC CCGGAGTGCT GCGGGACGCC 
    CCTCAGTACT TATCGGGACA GCTTACAAGC CAAAGCCTTA GTAGAAGAAT TGAGCCTTTT
    AACCCTAGAG TTCAGGTCAA ACTTTGGTAT GACAAAGTGG GCCATCAGCT TATAGTCACC
    ATACTGGGTG CAAAGGATCT GCCTTCAAGG GAAGACGGTC GACCTCGCAA TCCCTATGTC
    AAGATCTACT TCCTCCCTGA CAGAAGCGAC AAAAGCAAAA GAAGAACAAA AACGGTAAAG
    AAATCACTAG AGCCCAAATG GAACCAGACC TTTATGTACT CGCCCGTGCA CCGGCGGGAG
    TTCAGGGAGA GAATGCTGGA GATCACGCTC TGGGACCAGG CCCGGGTTCG GGAGGAGGAG
    AGCGAGTTCC TCGGGGAGAT CCTGATCGAG CTGGAAACGG CGCTGGTGGA CGACGAGCCA
    CACTGGTATA AACTGCAGAC GCATGACGTG TCGTCGGTCC CGCTGCCCAA GAGCTCCCCC
    TCCTTGCAGA GACGAGCACT GCATGGGGAC AGTCCCACAC GCAGACTGCA GAGGTCTCAG
    AGGATCAGCG ACAGCGAGTT CTCAGACTAT GACTGTGAGG ATGGCGTTGG CGTCGTATCA
    GACTATAGAA ACGGCAGGGA CCTCCAGAGC TCCACCCTCT CTGTGCCTGA GCAAGTCTTA
    TCGTCCAATC ACTGCTCTCG GTCTGCTGAT ATCAACAGAG CGCGCTCGCG ATCTCCCAGC
    ATGCCTCCTT CTCAGAGTCA TTTTCTCACG TTACCTCGAA CCCGACACAG TCAGCCTCAT
    GACAGTGACC TGCAGGATCT CAGGAATTAC GGAGGAGCCC CGGACCGGCA CGAGTACCAC
    CATCGATCTC GTTCTGCAGA CCAACGGGCC GCAATGGAGC GTCCCATGTA CAGTCGCTCT
    CGCTCCACAG AGCGTCCGCC CGACGGACCG CACATGAGGT CCTCCATGCC CTCTCTGCCA
    TCGGGACACT CTGCACCATC TTCCCCTGCC TTATCGAGGG CTCATCCTCG CGGTGGATCG
    GTTCAGACCA GCCCCACAGG GACCCCCATA AACAGCCGGA GAGGAAGACA GCTGCCACAA
    CTGCCTCCAA AGGGGACGCT GGAGAGAAAA GATGGAGAAG AAGAAGTTGA GGCTTGCCCA
    GACTCGGACT CCCCCAGTCG GACAAACTCA GGTGCTCTGG ATGTTGAGGA GCGAACGCGG
    CAGATGAAGC TGAAGATGAA CAAATATAAA CAGGGGACCG GCTCTGATTC AAGACTGGAG
    CAGGACTATC ACAAGCGCTC CGGCAGGGAT CCTCAGCGTG GAGAAAACCT GTCGGCCAAG
    TCTTCGGACA GTGATGTGAG CGACGTGTCC GCTGTGTCGA GAACCAGCAG TGCATCTCGA
    TTCAGCAGCA TGAGCTACAT CTCTGTCCAG TCGGAGCGGC CACGGGGCAG CAGGAAGATC
    AGTGAATTTA CATCCAAAAT GAAAAACAGG CAAATGGGAA CGGCGGCTGG AGGCAACATG
    ACCAAGAGCA CCAGCATCGG CGGAGACATG TGCAGCCTGG AGAAGACCGA CGGCAGCCAG
    TCGGACACGG CCGTAAGCCT GGTGGGAACG GACGACAAGA AACGGCGCTC CAGCATCGGA
    GCTAAGATGG CGGCAGTGGT GGGATTGAGC CGAAAGAGTC GCAGCGCTTC ACAGCTCGGT
    CAGACAGAAG CAGGAGGGAA GAAGCTGCGC AGCACCATCC AGAGGAGCAC AGAGACGGGA
    CTGGCTGTGG AGATGAGGAG CCGAATGACA AGACAAGCCA GCCGAGAATC CACTGACGGC
    AGCATGAACA GCTACAGCTC TGAGGGAAAC CTGATCTTCC CTGGAGTGAG ACTCTCTTCA
    GACAGTCAGT TCAGTGATTT CCTGGATGGA CTTGGCCCAG CCCAGCTGGT GGGCAGACAG
    ACATTAGCCA CACCTCCTAT GGGTGACATT CAGATCGGGA TGGTGGAAAA GAAAGGAGCC
    CTAGAGGTGG AGGTGATCCG AGCCCGAGGC CTTGTGGGAA AACCAGGTTC AAAGGCACTG
    CCAGCCCCTT ACGTAAAGGT GTATCTACTG GAGAACGGCG CCTGCATAGC CAAAAAGAAA
    ACAAAAGTAG CAAGAAAAAC ACTGGATCCT CTTTACCAGC AGCAGCTGTC ATTCGAAGAG
    TCTCCAGGAG GAAAAGTTTT ACAGATTATA GTGTGGGGAG ATTATGGACG TATGGACCAC
    AAATCTTTTA TGGGAGCTGC TCAGATACTG TTAGATGATC TGGACTTGTC CAACATGGTT
    ATTGGCTGGT TTAAGCTGTT TCCCCCATCT TCACTGGTCG ACCCAACCTT AGCCCCTTTG
    ACCAGAAGAG CTTCCCAGAC ATCTCTGGAC AGTGGGTCCG GGCCATTTGC TCGCTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149447
    Clone ID Related Accession (Same CDS sequence) XM_021467011.1
    Accession Version XM_021467011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGGTAT GCAACCTGTG CCGAAAACAA CAAGAAATCC TCACCAAGTC AGGGGCCTGG 
    TTCTTCAGTG GCCCAGGGGG AGCCCTGCCT GCAGGTGCGA GTGACCCTCG CCGCCGCCAC
    GAAGAGGCTC CGCAGGAGAA GAAGGCCAAA CTGCAGGAAG CTCCATTGCT GTACCAGAGC
    TCAGGAGACC GCAGCCGAGC TCCAGGACTC CCCCGACAGG CATCCCTCAA CAACAACTCT
    AGTCTGAAGC CCAACGACCC AACAAGCAAC AGGAAAAGAA GCCCATCCGC AACCAGGGAT
    CCAAACCAAA AATACAACCA AAGGGAGGAG AAGGCGGATC GTTCGCAGTA TGCACCAGGA
    GAGGGCGGCA TGCCACGATC GCCGTCTGAC TATGGAGCCG GAGATCGGCA GTGGCGGGGA
    GGCTGCGGGC GCTCCTATGA GGACGCGGAG GTAGCGCGAG GGGCGTACCG GGCTCGACGG
    GGCGGCTGGC ACTCTCAGGA GGAATACCCA CCCGAACCTG GCTTCCTACC CGATGGACCA
    CCCCTCAGCG AGCACGAGCT CCAGCGCCAG CGGCAGGAGG AGTACCAGAA CCGCTACCGC
    AGTGACCCAA ACCTGGCTCG ATACCCTGTC AAACCACAGC CCTACGAGGA GCAGATGCGC
    ATGCACGCGG AGGTCTCCAG ATTTAGGCAT GAACGGCGGC ACAGTGATGT CTCCCTGGCG
    TACACTGAGA TGGAAGAGCC CCTGGGGCCT CTGCGGGGTC CACACTACTC ACCGGGCTCC
    GGACAAACGC ACTGGAGGTC CAACCACAGT CCCCCTGGCA CAGACTCGGC CCACCGGCAG
    CAGCAGCTCC TGGATCCCGT CTCGGCTGTA CGGAAGAGTA AAAGGGAAAA GATGGAGAGC
    ATGTTGAGGA ACGACTCGCT CAGTTCCGAC CAATCGGAGT CCGTCAGGCC GCCGCCACCC
    AAACCTCACA AGACAAAGCG AGGCGGGAAA ATGAGGCAGG TTTCACTGAG CAGCTCTGAG
    GAGGAACTGG CCACAACGCC CGAGTACACC AGCTGCGAGG ATGTGGAGAT TGAGAGCGAG
    TCAGTCAGTG AGAAAGGGGA CAGTCAAAGG GGAAAAAGAC AAACTTTTGA GCAGTCACAC
    ACCTTATCTG AGGGCCAAAA GAAAATGGTG CGCTTTGGGG GCCACTCTTG GGAAGAAGAT
    GCTGAGTGGT GTGAGCCCCA GGTTAAAGAC TCGGGTGTTG ATACGTGCAG TAGCACAACC
    CTAAACGAGG AGCATAGCCA TAGTGAGAAG CACCCAGTTA CATGGCAGCC CTCCAAAGAC
    GGAGATCGGC TCATCGGCAG GATCCTGCTC AACAAGCGCA TGAAGGACGG CAGCGTTCCC
    CGCGATTCCG GAGCTCTTCT GGGACTCAAA GTGGTTGGTG GAAAGATGAC AGAGTCTGGG
    CGACTTTGTG CGTTCATTAC CAAAGTAAGG AAAGGGAGTT TAGCGGATAC AGTTGGGCAC
    CTCAGACCAG GTGACCAGGT CCTGGAGTGG AATGGCCGAC AGTTACAAGG AGCCACATTT
    AAAGAAGTTT ACAATATCAT TTTAGAATCA AAGCCAGAGC CTCAGGTGGA ACTAGTGGTC
    TCACGACCCA TAGGAGATAT ACCCAGGATA CCAGAAAGCA CACACGCACA ACTTGAGTCC
    AGTTCGAGTT CATTTGAGTC TCAGAAGATG GAGCGTCCCT CCATATCCGT CACGTCTCCC
    ATGAGCCCCG GAGTGCTGCG GGACGCCCCT CAGTACTTAT CGGGACAGCT TACAAGCCAA
    AGCCTTAGTA GAAGAATTGA GCCTTTTAAC CCTAGAGTTC AGGTCAAACT TTGGTATGAC
    AAAGTGGGCC ATCAGCTTAT AGTCACCATA CTGGGTGCAA AGGATCTGCC TTCAAGGGAA
    GACGGTCGAC CTCGCAATCC CTATGTCAAG ATCTACTTCC TCCCTGACAG AAGCGACAAA
    AGCAAAAGAA GAACAAAAAC GGTAAAGAAA TCACTAGAGC CCAAATGGAA CCAGACCTTT
    ATGTACTCGC CCGTGCACCG GCGGGAGTTC AGGGAGAGAA TGCTGGAGAT CACGCTCTGG
    GACCAGGCCC GGGTTCGGGA GGAGGAGAGC GAGTTCCTCG GGGAGATCCT GATCGAGCTG
    GAAACGGCGC TGGTGGACGA CGAGCCACAC TGGTATAAAC TGCAGACGCA TGACGTGTCG
    TCGGTCCCGC TGCCCAAGAG CTCCCCCTCC TTGCAGAGAC GAGCACTGCA TGGGGACAGT
    CCCACACGCA GACTGCAGAC CCAAGGCCCG TTTGTGTACA GCTCAGGGTC TCAGAGGATC
    AGCGACAGCG AGTTCTCAGA CTATGACTGT GAGGATGGCG TTGGCGTCGT ATCAGACTAT
    AGAAACGGCA GGGACCTCCA GAGCTCCACC CTCTCTGTGC CTGAGCAAGT CTTATCGTCC
    AATCACTGCT CTCGGTCTGC TGATATCAAC AGAGCGCGCT CGCGATCTCC CAGCATGCCT
    CCTTCTCAGA GTCATTTTCT CACGTTACCT CGAACCCGAC ACAGTCAGCC TCATGACAGT
    GACCTGCAGG ATCTCAGGAA TTACGGAGGA GCCCCGGACC GGCACGAGTA CCACCATCGA
    TCTCGTTCTG CAGACCAACG GGCCGCAATG GAGCGTCCCA TGTACAGTCG CTCTCGCTCC
    ACAGAGCGTC CGCCCGACGG ACCGCACATG AGGTCCTCCA TGCCCTCTCT GCCATCGGGA
    CACTCTGCAC CATCTTCCCC TGCCTTATCG AGGGCTCATC CTCGCGGTGG ATCGGTTCAG
    ACCAGCCCCA CAGGGACCCC CATAAACAGC CGGAGAGGAA GACAGCTGCC ACAACTGCCT
    CCAAAGGGGA CGCTGGAGAG AAAAGATGGA GAAGAAGAAG TTGAGGCTTG CCCAGACTCG
    GACTCCCCCA GTCGGACAAA CTCAGGTGCT CTGGATGTTG AGGAGCGAAC GCGGCAGATG
    AAGCTGAAGA TGAACAAATA TAAACAGGGG ACCGGCTCTG ATTCAAGACT GGAGCAGGAC
    TATCACAAGC GCTCCGGCAG GGATCCTCAG CGTGGAGAAA ACCTGTCGGC CAAGTCTTCG
    GACAGTGATG TGAGCGACGT GTCCGCTGTG TCGAGAACCA GCAGTGCATC TCGATTCAGC
    AGCATGAGCT ACATCTCTGT CCAGTCGGAG CGGCCACGGG GCAGCAGGAA GATCAGTGAA
    TTTACATCCA AAATGAAAAA CAGGCAAATG GGAACGGCGG CTGGAGGCAA CATGACCAAG
    AGCACCAGCA TCGGCGGAGA CATGTGCAGC CTGGAGAAGA CCGACGGCAG CCAGTCGGAC
    ACGGCCGTAA GCCTGGTGGG AACGGACGAC AAGAAACGGC GCTCCAGCAT CGGAGCTAAG
    ATGGCGGCAG TGGTGGGATT GAGCCGAAAG AGTCGCAGCG CTTCACAGCT CGGTCAGACA
    GAAGCAGGAG GGAAGAAGCT GCGCAGCACC ATCCAGAGGA GCACAGAGAC GGGACTGGCT
    GTGGAGATGA GGAGCCGAAT GACAAGACAA GCCAGCCGAG AATCCACTGA CGGCAGCATG
    AACAGCTACA GCTCTGAGGG AAACCTGATC TTCCCTGGAG TGAGACTCTC TTCAGACAGT
    CAGTTCAGTG ATTTCCTGGA TGGACTTGGC CCAGCCCAGC TGGTGGGCAG ACAGACATTA
    GCCACACCTC CTATGGGTGA CATTCAGATC GGGATGGTGG AAAAGAAAGG AGCCCTAGAG
    GTGGAGGTGA TCCGAGCCCG AGGCCTTGTG GGAAAACCAG GTTCAAAGGC ACTGCCAGCC
    CCTTACGTAA AGGTGTATCT ACTGGAGAAC GGCGCCTGCA TAGCCAAAAA GAAAACAAAA
    GTAGCAAGAA AAACACTGGA TCCTCTTTAC CAGCAGCAGC TGTCATTCGA AGAGTCTCCA
    GGAGGAAAAG TTTTACAGAT TATAGTGTGG GGAGATTATG GACGTATGGA CCACAAATCT
    TTTATGGGAG CTGCTCAGAT ACTGTTAGAT GATCTGGACT TGTCCAACAT GGTTATTGGC
    TGGTTTAAGC TGTTTCCCCC ATCTTCACTG GTCGACCCAA CCTTAGCCCC TTTGACCAGA
    AGAGCTTCCC AGACATCTCT GGACAGTGGG TCCGGGCCAT TTGCTCGCTC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322686.1
    CDS250..4443
    Translation

    Target ORF information:

    RefSeq Version XM_021467011.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGGTAT GCAACCTGTG CCGAAAACAA CAAGAAATCC TCACCAAGTC AGGGGCCTGG 
    TTCTTCAGTG GCCCAGGGGG AGCCCTGCCT GCAGGTGCGA GTGACCCTCG CCGCCGCCAC
    GAAGAGGCTC CGCAGGAGAA GAAGGCCAAA CTGCAGGAAG CTCCATTGCT GTACCAGAGC
    TCAGGAGACC GCAGCCGAGC TCCAGGACTC CCCCGACAGG CATCCCTCAA CAACAACTCT
    AGTCTGAAGC CCAACGACCC AACAAGCAAC AGGAAAAGAA GCCCATCCGC AACCAGGGAT
    CCAAACCAAA AATACAACCA AAGGGAGGAG AAGGCGGATC GTTCGCAGTA TGCACCAGGA
    GAGGGCGGCA TGCCACGATC GCCGTCTGAC TATGGAGCCG GAGATCGGCA GTGGCGGGGA
    GGCTGCGGGC GCTCCTATGA GGACGCGGAG GTAGCGCGAG GGGCGTACCG GGCTCGACGG
    GGCGGCTGGC ACTCTCAGGA GGAATACCCA CCCGAACCTG GCTTCCTACC CGATGGACCA
    CCCCTCAGCG AGCACGAGCT CCAGCGCCAG CGGCAGGAGG AGTACCAGAA CCGCTACCGC
    AGTGACCCAA ACCTGGCTCG ATACCCTGTC AAACCACAGC CCTACGAGGA GCAGATGCGC
    ATGCACGCGG AGGTCTCCAG ATTTAGGCAT GAACGGCGGC ACAGTGATGT CTCCCTGGCG
    TACACTGAGA TGGAAGAGCC CCTGGGGCCT CTGCGGGGTC CACACTACTC ACCGGGCTCC
    GGACAAACGC ACTGGAGGTC CAACCACAGT CCCCCTGGCA CAGACTCGGC CCACCGGCAG
    CAGCAGCTCC TGGATCCCGT CTCGGCTGTA CGGAAGAGTA AAAGGGAAAA GATGGAGAGC
    ATGTTGAGGA ACGACTCGCT CAGTTCCGAC CAATCGGAGT CCGTCAGGCC GCCGCCACCC
    AAACCTCACA AGACAAAGCG AGGCGGGAAA ATGAGGCAGG TTTCACTGAG CAGCTCTGAG
    GAGGAACTGG CCACAACGCC CGAGTACACC AGCTGCGAGG ATGTGGAGAT TGAGAGCGAG
    TCAGTCAGTG AGAAAGGGGA CAGTCAAAGG GGAAAAAGAC AAACTTTTGA GCAGTCACAC
    ACCTTATCTG AGGGCCAAAA GAAAATGGTG CGCTTTGGGG GCCACTCTTG GGAAGAAGAT
    GCTGAGTGGT GTGAGCCCCA GGTTAAAGAC TCGGGTGTTG ATACGTGCAG TAGCACAACC
    CTAAACGAGG AGCATAGCCA TAGTGAGAAG CACCCAGTTA CATGGCAGCC CTCCAAAGAC
    GGAGATCGGC TCATCGGCAG GATCCTGCTC AACAAGCGCA TGAAGGACGG CAGCGTTCCC
    CGCGATTCCG GAGCTCTTCT GGGACTCAAA GTGGTTGGTG GAAAGATGAC AGAGTCTGGG
    CGACTTTGTG CGTTCATTAC CAAAGTAAGG AAAGGGAGTT TAGCGGATAC AGTTGGGCAC
    CTCAGACCAG GTGACCAGGT CCTGGAGTGG AATGGCCGAC AGTTACAAGG AGCCACATTT
    AAAGAAGTTT ACAATATCAT TTTAGAATCA AAGCCAGAGC CTCAGGTGGA ACTAGTGGTC
    TCACGACCCA TAGGAGATAT ACCCAGGATA CCAGAAAGCA CACACGCACA ACTTGAGTCC
    AGTTCGAGTT CATTTGAGTC TCAGAAGATG GAGCGTCCCT CCATATCCGT CACGTCTCCC
    ATGAGCCCCG GAGTGCTGCG GGACGCCCCT CAGTACTTAT CGGGACAGCT TACAAGCCAA
    AGCCTTAGTA GAAGAATTGA GCCTTTTAAC CCTAGAGTTC AGGTCAAACT TTGGTATGAC
    AAAGTGGGCC ATCAGCTTAT AGTCACCATA CTGGGTGCAA AGGATCTGCC TTCAAGGGAA
    GACGGTCGAC CTCGCAATCC CTATGTCAAG ATCTACTTCC TCCCTGACAG AAGCGACAAA
    AGCAAAAGAA GAACAAAAAC GGTAAAGAAA TCACTAGAGC CCAAATGGAA CCAGACCTTT
    ATGTACTCGC CCGTGCACCG GCGGGAGTTC AGGGAGAGAA TGCTGGAGAT CACGCTCTGG
    GACCAGGCCC GGGTTCGGGA GGAGGAGAGC GAGTTCCTCG GGGAGATCCT GATCGAGCTG
    GAAACGGCGC TGGTGGACGA CGAGCCACAC TGGTATAAAC TGCAGACGCA TGACGTGTCG
    TCGGTCCCGC TGCCCAAGAG CTCCCCCTCC TTGCAGAGAC GAGCACTGCA TGGGGACAGT
    CCCACACGCA GACTGCAGAC CCAAGGCCCG TTTGTGTACA GCTCAGGGTC TCAGAGGATC
    AGCGACAGCG AGTTCTCAGA CTATGACTGT GAGGATGGCG TTGGCGTCGT ATCAGACTAT
    AGAAACGGCA GGGACCTCCA GAGCTCCACC CTCTCTGTGC CTGAGCAAGT CTTATCGTCC
    AATCACTGCT CTCGGTCTGC TGATATCAAC AGAGCGCGCT CGCGATCTCC CAGCATGCCT
    CCTTCTCAGA GTCATTTTCT CACGTTACCT CGAACCCGAC ACAGTCAGCC TCATGACAGT
    GACCTGCAGG ATCTCAGGAA TTACGGAGGA GCCCCGGACC GGCACGAGTA CCACCATCGA
    TCTCGTTCTG CAGACCAACG GGCCGCAATG GAGCGTCCCA TGTACAGTCG CTCTCGCTCC
    ACAGAGCGTC CGCCCGACGG ACCGCACATG AGGTCCTCCA TGCCCTCTCT GCCATCGGGA
    CACTCTGCAC CATCTTCCCC TGCCTTATCG AGGGCTCATC CTCGCGGTGG ATCGGTTCAG
    ACCAGCCCCA CAGGGACCCC CATAAACAGC CGGAGAGGAA GACAGCTGCC ACAACTGCCT
    CCAAAGGGGA CGCTGGAGAG AAAAGATGGA GAAGAAGAAG TTGAGGCTTG CCCAGACTCG
    GACTCCCCCA GTCGGACAAA CTCAGGTGCT CTGGATGTTG AGGAGCGAAC GCGGCAGATG
    AAGCTGAAGA TGAACAAATA TAAACAGGGG ACCGGCTCTG ATTCAAGACT GGAGCAGGAC
    TATCACAAGC GCTCCGGCAG GGATCCTCAG CGTGGAGAAA ACCTGTCGGC CAAGTCTTCG
    GACAGTGATG TGAGCGACGT GTCCGCTGTG TCGAGAACCA GCAGTGCATC TCGATTCAGC
    AGCATGAGCT ACATCTCTGT CCAGTCGGAG CGGCCACGGG GCAGCAGGAA GATCAGTGAA
    TTTACATCCA AAATGAAAAA CAGGCAAATG GGAACGGCGG CTGGAGGCAA CATGACCAAG
    AGCACCAGCA TCGGCGGAGA CATGTGCAGC CTGGAGAAGA CCGACGGCAG CCAGTCGGAC
    ACGGCCGTAA GCCTGGTGGG AACGGACGAC AAGAAACGGC GCTCCAGCAT CGGAGCTAAG
    ATGGCGGCAG TGGTGGGATT GAGCCGAAAG AGTCGCAGCG CTTCACAGCT CGGTCAGACA
    GAAGCAGGAG GGAAGAAGCT GCGCAGCACC ATCCAGAGGA GCACAGAGAC GGGACTGGCT
    GTGGAGATGA GGAGCCGAAT GACAAGACAA GCCAGCCGAG AATCCACTGA CGGCAGCATG
    AACAGCTACA GCTCTGAGGG AAACCTGATC TTCCCTGGAG TGAGACTCTC TTCAGACAGT
    CAGTTCAGTG ATTTCCTGGA TGGACTTGGC CCAGCCCAGC TGGTGGGCAG ACAGACATTA
    GCCACACCTC CTATGGGTGA CATTCAGATC GGGATGGTGG AAAAGAAAGG AGCCCTAGAG
    GTGGAGGTGA TCCGAGCCCG AGGCCTTGTG GGAAAACCAG GTTCAAAGGC ACTGCCAGCC
    CCTTACGTAA AGGTGTATCT ACTGGAGAAC GGCGCCTGCA TAGCCAAAAA GAAAACAAAA
    GTAGCAAGAA AAACACTGGA TCCTCTTTAC CAGCAGCAGC TGTCATTCGA AGAGTCTCCA
    GGAGGAAAAG TTTTACAGAT TATAGTGTGG GGAGATTATG GACGTATGGA CCACAAATCT
    TTTATGGGAG CTGCTCAGAT ACTGTTAGAT GATCTGGACT TGTCCAACAT GGTTATTGGC
    TGGTTTAAGC TGTTTCCCCC ATCTTCACTG GTCGACCCAA CCTTAGCCCC TTTGACCAGA
    AGAGCTTCCC AGACATCTCT GGACAGTGGG TCCGGGCCAT TTGCTCGCTC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149448
    Clone ID Related Accession (Same CDS sequence) XM_017351550.2
    Accession Version XM_017351550.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAGTTTG AGACATTGCG TCAGTTCTGT AGCTCGGTTT TATCTCATTT TAATGGGGCT 
    TTCACGCCGC CGCAGAATAT TCTGCAGACA GAGCTCCTGG AGCAGGCGCT CAGCACCATC
    GGGAAAAGAA GCCCATCCGC AACCAGGGAT CCAAACCAAA AATACAACCA AAGGGAGGAG
    AAGGCGGATC GTTCGCAGTA TGCACCAGGA GAGGGCGGCA TGCCACGATC GCCGTCTGAC
    TATGGAGCCG GAGATCGGCA GTGGCGGGGA GGCTGCGGGC GCTCCTATGA GGACGCGGAG
    GTAGCGCGAG GGGCGTACCG GGCTCGACGG GGCGGCTGGC ACTCTCAGGA GGAATACCCA
    CCCGAACCTG GCTTCCTACC CGATGGACCA CCCCTCAGCG AGCACGAGCT CCAGCGCCAG
    CGGCAGGAGG AGTACCAGAA CCGCTACCGC AGTGACCCAA ACCTGGCTCG ATACCCTGTC
    AAACCACAGC CCTACGAGGA GCAGATGCGC ATGCACGCGG AGGTCTCCAG ATTTAGGCAT
    GAACGGCGGC ACAGTGATGT CTCCCTGGCG TACACTGAGA TGGAAGAGCC CCTGGGGCCT
    CTGCGGGGTC CACACTACTC ACCGGGCTCC GGACAAACGC ACTGGAGGTC CAACCACAGT
    CCCCCTGGCA CAGACTCGGC CCACCGGCAG CAGCAGCTCC TGGATCCCGT CTCGGCTGTA
    CGGAAGAGTA AAAGGGAAAA GATGGAGAGC ATGTTGAGGA ACGACTCGCT CAGTTCCGAC
    CAATCGGAGT CCGTCAGGCC GCCGCCACCC AAACCTCACA AGACAAAGCG AGGCGGGAAA
    ATGAGGCAGG TTTCACTGAG CAGCTCTGAG GAGGAACTGG CCACAACGCC CGAGTACACC
    AGCTGCGAGG ATGTGGAGAT TGAGAGCGAG TCAGTCAGTG AGAAAGGGGA CAGTCAAAGG
    GGAAAAAGAC AAACTTTTGA GCAGTCACAC ACCTTATCTG AGGGCCAAAA GAAAATGGTG
    CGCTTTGGGG GCCACTCTTG GGAAGAAGAT GCTGAGTGGT GTGAGCCCCA GGTTAAAGAC
    TCGGGTGTTG ATACGTGCAG TAGCACAACC CTAAACGAGG AGCATAGCCA TAGTGAGAAG
    CACCCAGTTA CATGGCAGCC CTCCAAAGAC GGAGATCGGC TCATCGGCAG GATCCTGCTC
    AACAAGCGCA TGAAGGACGG CAGCGTTCCC CGCGATTCCG GAGCTCTTCT GGGACTCAAA
    GTGGTTGGTG GAAAGATGAC AGAGTCTGGG CGACTTTGTG CGTTCATTAC CAAAGTAAGG
    AAAGGGAGTT TAGCGGATAC AGTTGGGCAC CTCAGACCAG GTGACCAGGT CCTGGAGTGG
    AATGGCCGAC AGTTACAAGG AGCCACATTT AAAGAAGTTT ACAATATCAT TTTAGAATCA
    AAGCCAGAGC CTCAGGTGGA ACTAGTGGTC TCACGACCCA TAGGAGATAT ACCCAGGATA
    CCAGAAAGCA CACACGCACA ACTTGAGTCC AGTTCGAGTT CATTTGAGTC TCAGAAGATG
    GAGCGTCCCT CCATATCCGT CACGTCTCCC ATGAGCCCCG GAGTGCTGCG GGACGCCCCT
    CAGTACTTAT CGGGACAGCT TACAAGCCAA AGCCTTAGTA GAAGAATTGA GCCTTTTAAC
    CCTAGAGTTC AGGTCAAACT TTGGTATGAC AAAGTGGGCC ATCAGCTTAT AGTCACCATA
    CTGGGTGCAA AGGATCTGCC TTCAAGGGAA GACGGTCGAC CTCGCAATCC CTATGTCAAG
    ATCTACTTCC TCCCTGACAG AAGCGACAAA AGCAAAAGAA GAACAAAAAC GGTAAAGAAA
    TCACTAGAGC CCAAATGGAA CCAGACCTTT ATGTACTCGC CCGTGCACCG GCGGGAGTTC
    AGGGAGAGAA TGCTGGAGAT CACGCTCTGG GACCAGGCCC GGGTTCGGGA GGAGGAGAGC
    GAGTTCCTCG GGGAGATCCT GATCGAGCTG GAAACGGCGC TGGTGGACGA CGAGCCACAC
    TGGTATAAAC TGCAGACGCA TGACGTGTCG TCGGTCCCGC TGCCCAAGAG CTCCCCCTCC
    TTGCAGAGAC GAGCACTGCA TGGGGACAGT CCCACACGCA GACTGCAGAC CCAAGGCCCG
    TTTGTGTACA GCTCAGGGTC TCAGAGGATC AGCGACAGCG AGTTCTCAGA CTATGACTGT
    GAGGATGGCG TTGGCGTCGT ATCAGACTAT AGAAACGGCA GGGACCTCCA GAGCTCCACC
    CTCTCTGTGC CTGAGCAAGT CTTATCGTCC AATCACTGCT CTCGGTCTGC TGATATCAAC
    AGAGCGCGCT CGCGATCTCC CAGCATGCCT CCTTCTCAGA GTCATTTTCT CACGTTACCT
    CGAACCCGAC ACAGTCAGCC TCATGACAGT GACCTGCAGG ATCTCAGGAA TTACGGAGGA
    GCCCCGGACC GGCACGAGTA CCACCATCGA TCTCGTTCTG CAGACCAACG GGCCGCAATG
    GAGCGTCCCA TGTACAGTCG CTCTCGCTCC ACAGAGCGTC CGCCCGACGG ACCGCACATG
    AGGTCCTCCA TGCCCTCTCT GCCATCGGGA CACTCTGCAC CATCTTCCCC TGCCTTATCG
    AGGGCTCATC CTCGCGGTGG ATCGGTTCAG ACCAGCCCCA CAGGGACCCC CATAAACAGC
    CGGAGAGGAA GACAGCTGCC ACAACTGCCT CCAAAGGGGA CGCTGGAGAG AAAAGATGGA
    GAAGAAGAAG TTGAGGCTTG CCCAGACTCG GACTCCCCCA GTCGGACAAA CTCAGGTGCT
    CTGGATGTTG AGGAGCGAAC GCGGCAGATG AAGCTGAAGA TGAACAAATA TAAACAGGGG
    ACCGGCTCTG ATTCAAGACT GGAGCAGGAC TATCACAAGC GCTCCGGCAG GGATCCTCAG
    CGTGGAGAAA ACCTGTCGGC CAAGTCTTCG GACAGTGATG TGAGCGACGT GTCCGCTGTG
    TCGAGAACCA GCAGTGCATC TCGATTCAGC AGCATGAGCT ACATCTCTGT CCAGTCGGAG
    CGGCCACGGG GCAGCAGGAA GATCAGTGAA TTTACATCCA AAATGAAAAA CAGGCAAATG
    GGAACGGCGG CTGGAGGCAA CATGACCAAG AGCACCAGCA TCGGCGGAGA CATGTGCAGC
    CTGGAGAAGA CCGACGGCAG CCAGTCGGAC ACGGCCGTAA GCCTGGTGGG AACGGACGAC
    AAGAAACGGC GCTCCAGCAT CGGAGCTAAG ATGGCGGCAG TGGTGGGATT GAGCCGAAAG
    AGTCGCAGCG CTTCACAGCT CGGTCAGACA GAAGCAGGAG GGAAGAAGCT GCGCAGCACC
    ATCCAGAGGA GCACAGAGAC GGGACTGGCT GTGGAGATGA GGAGCCGAAT GACAAGACAA
    GCCAGCCGAG AATCCACTGA CGGCAGCATG AACAGCTACA GCTCTGAGGG AAACCTGATC
    TTCCCTGGAG TGAGACTCTC TTCAGACAGT CAGTTCAGTG ATTTCCTGGA TGGACTTGGC
    CCAGCCCAGC TGGTGGGCAG ACAGACATTA GCCACACCTC CTATGGGTGA CATTCAGATC
    GGGATGGTGG AAAAGAAAGG AGCCCTAGAG GTGGAGGTGA TCCGAGCCCG AGGCCTTGTG
    GGAAAACCAG GTTCAAAGGC ACTGCCAGCC CCTTACGTAA AGGTGTATCT ACTGGAGAAC
    GGCGCCTGCA TAGCCAAAAA GAAAACAAAA GTAGCAAGAA AAACACTGGA TCCTCTTTAC
    CAGCAGCAGC TGTCATTCGA AGAGTCTCCA GGAGGAAAAG TTTTACAGAT TATAGTGTGG
    GGAGATTATG GACGTATGGA CCACAAATCT TTTATGGGAG CTGCTCAGAT ACTGTTAGAT
    GATCTGGACT TGTCCAACAT GGTTATTGGC TGGTTTAAGC TGTTTCCCCC ATCTTCACTG
    GTCGACCCAA CCTTAGCCCC TTTGACCAGA AGAGCTTCCC AGACATCTCT GGACAGTGGG
    TCCGGGCCAT TTGCTCGCTC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207039.2
    CDS470..4513
    Translation

    Target ORF information:

    RefSeq Version XM_017351550.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351550.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAGTTTG AGACATTGCG TCAGTTCTGT AGCTCGGTTT TATCTCATTT TAATGGGGCT 
    TTCACGCCGC CGCAGAATAT TCTGCAGACA GAGCTCCTGG AGCAGGCGCT CAGCACCATC
    GGGAAAAGAA GCCCATCCGC AACCAGGGAT CCAAACCAAA AATACAACCA AAGGGAGGAG
    AAGGCGGATC GTTCGCAGTA TGCACCAGGA GAGGGCGGCA TGCCACGATC GCCGTCTGAC
    TATGGAGCCG GAGATCGGCA GTGGCGGGGA GGCTGCGGGC GCTCCTATGA GGACGCGGAG
    GTAGCGCGAG GGGCGTACCG GGCTCGACGG GGCGGCTGGC ACTCTCAGGA GGAATACCCA
    CCCGAACCTG GCTTCCTACC CGATGGACCA CCCCTCAGCG AGCACGAGCT CCAGCGCCAG
    CGGCAGGAGG AGTACCAGAA CCGCTACCGC AGTGACCCAA ACCTGGCTCG ATACCCTGTC
    AAACCACAGC CCTACGAGGA GCAGATGCGC ATGCACGCGG AGGTCTCCAG ATTTAGGCAT
    GAACGGCGGC ACAGTGATGT CTCCCTGGCG TACACTGAGA TGGAAGAGCC CCTGGGGCCT
    CTGCGGGGTC CACACTACTC ACCGGGCTCC GGACAAACGC ACTGGAGGTC CAACCACAGT
    CCCCCTGGCA CAGACTCGGC CCACCGGCAG CAGCAGCTCC TGGATCCCGT CTCGGCTGTA
    CGGAAGAGTA AAAGGGAAAA GATGGAGAGC ATGTTGAGGA ACGACTCGCT CAGTTCCGAC
    CAATCGGAGT CCGTCAGGCC GCCGCCACCC AAACCTCACA AGACAAAGCG AGGCGGGAAA
    ATGAGGCAGG TTTCACTGAG CAGCTCTGAG GAGGAACTGG CCACAACGCC CGAGTACACC
    AGCTGCGAGG ATGTGGAGAT TGAGAGCGAG TCAGTCAGTG AGAAAGGGGA CAGTCAAAGG
    GGAAAAAGAC AAACTTTTGA GCAGTCACAC ACCTTATCTG AGGGCCAAAA GAAAATGGTG
    CGCTTTGGGG GCCACTCTTG GGAAGAAGAT GCTGAGTGGT GTGAGCCCCA GGTTAAAGAC
    TCGGGTGTTG ATACGTGCAG TAGCACAACC CTAAACGAGG AGCATAGCCA TAGTGAGAAG
    CACCCAGTTA CATGGCAGCC CTCCAAAGAC GGAGATCGGC TCATCGGCAG GATCCTGCTC
    AACAAGCGCA TGAAGGACGG CAGCGTTCCC CGCGATTCCG GAGCTCTTCT GGGACTCAAA
    GTGGTTGGTG GAAAGATGAC AGAGTCTGGG CGACTTTGTG CGTTCATTAC CAAAGTAAGG
    AAAGGGAGTT TAGCGGATAC AGTTGGGCAC CTCAGACCAG GTGACCAGGT CCTGGAGTGG
    AATGGCCGAC AGTTACAAGG AGCCACATTT AAAGAAGTTT ACAATATCAT TTTAGAATCA
    AAGCCAGAGC CTCAGGTGGA ACTAGTGGTC TCACGACCCA TAGGAGATAT ACCCAGGATA
    CCAGAAAGCA CACACGCACA ACTTGAGTCC AGTTCGAGTT CATTTGAGTC TCAGAAGATG
    GAGCGTCCCT CCATATCCGT CACGTCTCCC ATGAGCCCCG GAGTGCTGCG GGACGCCCCT
    CAGTACTTAT CGGGACAGCT TACAAGCCAA AGCCTTAGTA GAAGAATTGA GCCTTTTAAC
    CCTAGAGTTC AGGTCAAACT TTGGTATGAC AAAGTGGGCC ATCAGCTTAT AGTCACCATA
    CTGGGTGCAA AGGATCTGCC TTCAAGGGAA GACGGTCGAC CTCGCAATCC CTATGTCAAG
    ATCTACTTCC TCCCTGACAG AAGCGACAAA AGCAAAAGAA GAACAAAAAC GGTAAAGAAA
    TCACTAGAGC CCAAATGGAA CCAGACCTTT ATGTACTCGC CCGTGCACCG GCGGGAGTTC
    AGGGAGAGAA TGCTGGAGAT CACGCTCTGG GACCAGGCCC GGGTTCGGGA GGAGGAGAGC
    GAGTTCCTCG GGGAGATCCT GATCGAGCTG GAAACGGCGC TGGTGGACGA CGAGCCACAC
    TGGTATAAAC TGCAGACGCA TGACGTGTCG TCGGTCCCGC TGCCCAAGAG CTCCCCCTCC
    TTGCAGAGAC GAGCACTGCA TGGGGACAGT CCCACACGCA GACTGCAGAC CCAAGGCCCG
    TTTGTGTACA GCTCAGGGTC TCAGAGGATC AGCGACAGCG AGTTCTCAGA CTATGACTGT
    GAGGATGGCG TTGGCGTCGT ATCAGACTAT AGAAACGGCA GGGACCTCCA GAGCTCCACC
    CTCTCTGTGC CTGAGCAAGT CTTATCGTCC AATCACTGCT CTCGGTCTGC TGATATCAAC
    AGAGCGCGCT CGCGATCTCC CAGCATGCCT CCTTCTCAGA GTCATTTTCT CACGTTACCT
    CGAACCCGAC ACAGTCAGCC TCATGACAGT GACCTGCAGG ATCTCAGGAA TTACGGAGGA
    GCCCCGGACC GGCACGAGTA CCACCATCGA TCTCGTTCTG CAGACCAACG GGCCGCAATG
    GAGCGTCCCA TGTACAGTCG CTCTCGCTCC ACAGAGCGTC CGCCCGACGG ACCGCACATG
    AGGTCCTCCA TGCCCTCTCT GCCATCGGGA CACTCTGCAC CATCTTCCCC TGCCTTATCG
    AGGGCTCATC CTCGCGGTGG ATCGGTTCAG ACCAGCCCCA CAGGGACCCC CATAAACAGC
    CGGAGAGGAA GACAGCTGCC ACAACTGCCT CCAAAGGGGA CGCTGGAGAG AAAAGATGGA
    GAAGAAGAAG TTGAGGCTTG CCCAGACTCG GACTCCCCCA GTCGGACAAA CTCAGGTGCT
    CTGGATGTTG AGGAGCGAAC GCGGCAGATG AAGCTGAAGA TGAACAAATA TAAACAGGGG
    ACCGGCTCTG ATTCAAGACT GGAGCAGGAC TATCACAAGC GCTCCGGCAG GGATCCTCAG
    CGTGGAGAAA ACCTGTCGGC CAAGTCTTCG GACAGTGATG TGAGCGACGT GTCCGCTGTG
    TCGAGAACCA GCAGTGCATC TCGATTCAGC AGCATGAGCT ACATCTCTGT CCAGTCGGAG
    CGGCCACGGG GCAGCAGGAA GATCAGTGAA TTTACATCCA AAATGAAAAA CAGGCAAATG
    GGAACGGCGG CTGGAGGCAA CATGACCAAG AGCACCAGCA TCGGCGGAGA CATGTGCAGC
    CTGGAGAAGA CCGACGGCAG CCAGTCGGAC ACGGCCGTAA GCCTGGTGGG AACGGACGAC
    AAGAAACGGC GCTCCAGCAT CGGAGCTAAG ATGGCGGCAG TGGTGGGATT GAGCCGAAAG
    AGTCGCAGCG CTTCACAGCT CGGTCAGACA GAAGCAGGAG GGAAGAAGCT GCGCAGCACC
    ATCCAGAGGA GCACAGAGAC GGGACTGGCT GTGGAGATGA GGAGCCGAAT GACAAGACAA
    GCCAGCCGAG AATCCACTGA CGGCAGCATG AACAGCTACA GCTCTGAGGG AAACCTGATC
    TTCCCTGGAG TGAGACTCTC TTCAGACAGT CAGTTCAGTG ATTTCCTGGA TGGACTTGGC
    CCAGCCCAGC TGGTGGGCAG ACAGACATTA GCCACACCTC CTATGGGTGA CATTCAGATC
    GGGATGGTGG AAAAGAAAGG AGCCCTAGAG GTGGAGGTGA TCCGAGCCCG AGGCCTTGTG
    GGAAAACCAG GTTCAAAGGC ACTGCCAGCC CCTTACGTAA AGGTGTATCT ACTGGAGAAC
    GGCGCCTGCA TAGCCAAAAA GAAAACAAAA GTAGCAAGAA AAACACTGGA TCCTCTTTAC
    CAGCAGCAGC TGTCATTCGA AGAGTCTCCA GGAGGAAAAG TTTTACAGAT TATAGTGTGG
    GGAGATTATG GACGTATGGA CCACAAATCT TTTATGGGAG CTGCTCAGAT ACTGTTAGAT
    GATCTGGACT TGTCCAACAT GGTTATTGGC TGGTTTAAGC TGTTTCCCCC ATCTTCACTG
    GTCGACCCAA CCTTAGCCCC TTTGACCAGA AGAGCTTCCC AGACATCTCT GGACAGTGGG
    TCCGGGCCAT TTGCTCGCTC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149449
    Clone ID Related Accession (Same CDS sequence) XM_021467012.1
    Accession Version XM_021467012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAATTGC ACCAGCAGTT TGAAATGTAC AAAGATCAAG TGCAGAAGAT GGGTGAGGAG 
    GCTCAGAGCG GCCCGGAGCA GAAGAGCGAT GCTCCTACCT GCGGAATCTG CCATAAAACC
    AAGTTTGCCG ACGGCTGTGG CCACCTCTGT TCTTATTGCC AAACCAAGTT CTGTGCTCGC
    TGTGGAGGAA GAGTTTCACT GCGCTCTAAC AAGGAGGATA AAGTGGTGAT GTGGGTATGC
    AACCTGTGCC GAAAACAACA AGAAATCCTC ACCAAGTCAG GGGCCTGGTT CTTCAGTGGC
    CCAGGGGGAG CCCTGCCTGC AGGTGCGAGT GACCCTCGCC GCCGCCACGA AGAGGCTCCG
    CAGGAGAAGA AGGCCAAACT GCAGGAAGCT CCATTGCTGT ACCAGAGCTC AGGAGACCGC
    AGCCGAGCTC CAGGACTCCC CCGACAGGCA TCCCTCAACA ACAACTCTAG TCTGAAGCCC
    AACGACCCAA CAAGCAACAG GAAAAGAAGC CCATCCGCAA CCAGGGATCC AAACCAAAAA
    TACAACCAAA GGGAGGAGAA GGCGGATCGT TCGCAGTATG CACCAGGAGA GGGCGGCATG
    CCACGATCGC CGTCTGACTA TGGAGCCGGA GATCGGCAGT GGCGGGGAGG CTGCGGGCGC
    TCCTATGAGG ACGCGGAGGT AGCGCGAGGG GCGTACCGGG CTCGACGGGG CGGCTGGCAC
    TCTCAGGAGG AATACCCACC CGAACCTGGC TTCCTACCCG ATGGACCACC CCTCAGCGAG
    CACGAGCTCC AGCGCCAGCG GCAGGAGGAG TACCAGAACC GCTACCGCAG TGACCCAAAC
    CTGGCTCGAT ACCCTGTCAA ACCACAGCCC TACGAGGAGC AGATGCGCAT GCACGCGGAG
    GTCTCCAGAT TTAGGCATGA ACGGCGGCAC AGTGATGTCT CCCTGGCGTA CACTGAGATG
    GAAGAGCCCC TGGGGCCTCT GCGGGGTCCA CACTACTCAC CGGGCTCCGG ACAAACGCAC
    TGGAGGTCCA ACCACAGTCC CCCTGGCACA GACTCGGCCC ACCGGCAGCA GCAGCTCCTG
    GATCCCGTCT CGGCTGTACG GAAGAGTAAA AGGGAAAAGA TGGAGAGCAT GTTGAGGAAC
    GACTCGCTCA GTTCCGACCA ATCGGAGTCC GTCAGGCCGC CGCCACCCAA ACCTCACAAG
    ACAAAGCGAG GCGGGAAAAT GAGGCAGGTT TCACTGAGCA GCTCTGAGGA GGAACTGGCC
    ACAACGCCCG AGTACACCAG CTGCGAGGAT GTGGAGATTG AGAGCGAGTC AGTCAGTGAG
    AAAGGGGACA GTCAAAGGGG AAAAAGACAA ACTTTTGAGC AGTCACACAC CTTATCTGAG
    GGCCAAAAGA AAATGGTGCG CTTTGGGGGC CACTCTTGGG AAGAAGATGC TGAGTGGTGT
    GAGCCCCAGG TTAAAGACTC GGGTGTTGAT ACGTGCAGTA GCACAACCCT AAACGAGGAG
    CATAGCCATA GTGAGAAGCA CCCAGTTACA TGGCAGCCCT CCAAAGACGG AGATCGGCTC
    ATCGGCAGGA TCCTGCTCAA CAAGCGCATG AAGGACGGCA GCGTTCCCCG CGATTCCGGA
    GCTCTTCTGG GACTCAAAGT GGTTGGTGGA AAGATGACAG AGTCTGGGCG ACTTTGTGCG
    TTCATTACCA AAGTAAGGAA AGGGAGTTTA GCGGATACAG TTGGGCACCT CAGACCAGGT
    GACCAGGTCC TGGAGTGGAA TGGCCGACAG TTACAAGGAG CCACATTTAA AGAAGTTTAC
    AATATCATTT TAGAATCAAA GCCAGAGCCT CAGGTGGAAC TAGTGGTCTC ACGACCCATA
    GGAGATATAC CCAGGATACC AGAAAGCACA CACGCACAAC TTGAGTCCAG TTCGAGTTCA
    TTTGAGTCTC AGAAGATGGA GCGTCCCTCC ATATCCGTCA CGTCTCCCAT GAGCCCCGGA
    GTGCTGCGGG ACGCCCCTCA GTACTTATCG GGACAGCTTA CAAGCCAAAG CCTTAGTAGA
    AGAATTGAGC CTTTTAACCC TAGAGTTCAG GTCAAACTTT GGTATGACAA AGTGGGCCAT
    CAGCTTATAG TCACCATACT GGGTGCAAAG GATCTGCCTT CAAGGGAAGA CGGTCGACCT
    CGCAATCCCT ATGTCAAGAT CTACTTCCTC CCTGACAGAA GCGACAAAAG CAAAAGAAGA
    ACAAAAACGG TAAAGAAATC ACTAGAGCCC AAATGGAACC AGACCTTTAT GTACTCGCCC
    GTGCACCGGC GGGAGTTCAG GGAGAGAATG CTGGAGATCA CGCTCTGGGA CCAGGCCCGG
    GTTCGGGAGG AGGAGAGCGA GTTCCTCGGG GAGATCCTGA TCGAGCTGGA AACGGCGCTG
    GTGGACGACG AGCCACACTG GTATAAACTG CAGACGCATG ACGTGTCGTC GGTCCCGCTG
    CCCAAGAGCT CCCCCTCCTT GCAGAGACGA GCACTGCATG GGGACAGTCC CACACGCAGA
    CTGCAGACCC AAGGCCCGTT TGTGTACAGC TCAGGGTCTC AGAGGATCAG CGACAGCGAG
    TTCTCAGACT ATGACTGTGA GGATGGCGTT GGCGTCGTAT CAGACTATAG AAACGGCAGG
    GACCTCCAGA GCTCCACCCT CTCTGTGCCT GAGCAAGTCT TATCGTCCAA TCACTGCTCT
    CGGTCTGCTG ATATCAACAG AGCGCGCTCG CGATCTCCCA GCATGCCTCC TTCTCAGAGT
    CATTTTCTCA CGTTACCTCG AACCCGACAC AGTCAGCCTC ATGACAGTGA CCTGCAGGAT
    CTCAGGAATT ACGGAGGAGC CCCGGACCGG CACGAGTACC ACCATCGATC TCGTTCTGCA
    GACCAACGGG CCGCAATGGA GCGTCCCATG TACAGTCGCT CTCGCTCCAC AGAGCGTCCG
    CCCGACGGAC CGCACATGAG GTCCTCCATG CCCTCTCTGC CATCGGGACA CTCTGCACCA
    TCTTCCCCTG CCTTATCGAG GGCTCATCCT CGCGGTGGAT CGGTTCAGAC CAGCCCCACA
    GGGACCCCCA TAAACAGCCG GAGAGGAAGA CAGCTGCCAC AACTGCCTCC AAAGGGGACG
    CTGGAGAGAA AAGATGGAGA AGAAGAAGTT GAGGCTTGCC CAGACTCGGA CTCCCCCAGT
    CGGACAAACT CAGGTGCTCT GGATGTTGAG GAGCGAACGC GGCAGATGAA GCTGAAGATG
    AACAAATATA AACAGGGGAC CGGCTCTGAT TCAAGACTGG AGCAGGACTA TCACAAGCGC
    TCCGGCAGGG ATCCTCAGCG TGGAGAAAAC CTGTCGGCCA AGTCTTCGGA CAGTGATGTG
    AGCGACGTGT CCGCTGTGTC GAGAACCAGC AGTGCATCTC GATTCAGCAG CATGAGCTAC
    ATCTCTGTCC AGTCGGAGCG GCCACGGGGC AGCAGGAAGA TCAGTGAATT TACATCCAAA
    ATGAAAAACA GGCAAATGGG AACGGCGGCT GGAGGCAACA TGACCAAGAG CACCAGCATC
    GGCGGAGACA TGTGCAGCCT GGAGAAGACC GACGGCAGCC AGTCGGACAC GGCCGTAAGC
    CTGGTGGGAA CGGACGACAA GAAACGGCGC TCCAGCATCG GAGCTAAGAT GGCGGCAGTG
    GTGGGATTGA GCCGAAAGAG TCGCAGCGCT TCACAGCTCG GTCAGACAGA AGCAGGAGGG
    AAGAAGCTGC GCAGCACCAT CCAGAGGAGC ACAGAGACGG GACTGGCTGT GGAGATGAGG
    AGCCGAATGA CAAGACAAGC CAGCCGAGAA TCCACTGACG GCAGCATGAA CAGCTACAGC
    TCTGAGGGAA ACCTGATCTT CCCTGGAGTG AGACTCTCTT CAGACAGTCA GTTCAGTGAT
    TTCCTGGATG GACTTGGCCC AGCCCAGCTG GTGGGCAGAC AGACATTAGC CACACCTCCT
    ATGGGTGACA TTCAGATCGG GATGGTGGAA AAGAAAGGAG CCCTAGAGGT GGAGGTGATC
    CGAGCCCGAG GCCTTGTGGG AAAACCAGGT TCAAAGGCAC TGCCAGCCCC TTACGTAAAG
    GTGTATCTAC TGGAGAACGG CGCCTGCATA GCCAAAAAGA AAACAAAAGT AGCAAGAAAA
    ACACTGGATC CTCTTTACCA GCAGCAGCTG TCATTCGAAG AGTCTCCAGG AGGAAAAGTT
    TTACAGATTA TAGTGTGGGG AGATTATGGA CGTATGGACC ACAAATCTTT TATGGGAGCT
    GCTCAGATAC TGTTAGATGA TCTGGACTTG TCCAACATGG TTATTGGCTG GTTTAAGCTG
    TTTCCCCCAT CTTCACTGGT CGACCCAACC TTAGCCCCTT TGACCAGAAG AGCTTCCCAG
    ACATCTCTGG ACAGTGGGTC CGGGCCATTT GCTCGCTCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322687.1
    CDS139..4560
    Translation

    Target ORF information:

    RefSeq Version XM_021467012.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAATTGC ACCAGCAGTT TGAAATGTAC AAAGATCAAG TGCAGAAGAT GGGTGAGGAG 
    GCTCAGAGCG GCCCGGAGCA GAAGAGCGAT GCTCCTACCT GCGGAATCTG CCATAAAACC
    AAGTTTGCCG ACGGCTGTGG CCACCTCTGT TCTTATTGCC AAACCAAGTT CTGTGCTCGC
    TGTGGAGGAA GAGTTTCACT GCGCTCTAAC AAGGAGGATA AAGTGGTGAT GTGGGTATGC
    AACCTGTGCC GAAAACAACA AGAAATCCTC ACCAAGTCAG GGGCCTGGTT CTTCAGTGGC
    CCAGGGGGAG CCCTGCCTGC AGGTGCGAGT GACCCTCGCC GCCGCCACGA AGAGGCTCCG
    CAGGAGAAGA AGGCCAAACT GCAGGAAGCT CCATTGCTGT ACCAGAGCTC AGGAGACCGC
    AGCCGAGCTC CAGGACTCCC CCGACAGGCA TCCCTCAACA ACAACTCTAG TCTGAAGCCC
    AACGACCCAA CAAGCAACAG GAAAAGAAGC CCATCCGCAA CCAGGGATCC AAACCAAAAA
    TACAACCAAA GGGAGGAGAA GGCGGATCGT TCGCAGTATG CACCAGGAGA GGGCGGCATG
    CCACGATCGC CGTCTGACTA TGGAGCCGGA GATCGGCAGT GGCGGGGAGG CTGCGGGCGC
    TCCTATGAGG ACGCGGAGGT AGCGCGAGGG GCGTACCGGG CTCGACGGGG CGGCTGGCAC
    TCTCAGGAGG AATACCCACC CGAACCTGGC TTCCTACCCG ATGGACCACC CCTCAGCGAG
    CACGAGCTCC AGCGCCAGCG GCAGGAGGAG TACCAGAACC GCTACCGCAG TGACCCAAAC
    CTGGCTCGAT ACCCTGTCAA ACCACAGCCC TACGAGGAGC AGATGCGCAT GCACGCGGAG
    GTCTCCAGAT TTAGGCATGA ACGGCGGCAC AGTGATGTCT CCCTGGCGTA CACTGAGATG
    GAAGAGCCCC TGGGGCCTCT GCGGGGTCCA CACTACTCAC CGGGCTCCGG ACAAACGCAC
    TGGAGGTCCA ACCACAGTCC CCCTGGCACA GACTCGGCCC ACCGGCAGCA GCAGCTCCTG
    GATCCCGTCT CGGCTGTACG GAAGAGTAAA AGGGAAAAGA TGGAGAGCAT GTTGAGGAAC
    GACTCGCTCA GTTCCGACCA ATCGGAGTCC GTCAGGCCGC CGCCACCCAA ACCTCACAAG
    ACAAAGCGAG GCGGGAAAAT GAGGCAGGTT TCACTGAGCA GCTCTGAGGA GGAACTGGCC
    ACAACGCCCG AGTACACCAG CTGCGAGGAT GTGGAGATTG AGAGCGAGTC AGTCAGTGAG
    AAAGGGGACA GTCAAAGGGG AAAAAGACAA ACTTTTGAGC AGTCACACAC CTTATCTGAG
    GGCCAAAAGA AAATGGTGCG CTTTGGGGGC CACTCTTGGG AAGAAGATGC TGAGTGGTGT
    GAGCCCCAGG TTAAAGACTC GGGTGTTGAT ACGTGCAGTA GCACAACCCT AAACGAGGAG
    CATAGCCATA GTGAGAAGCA CCCAGTTACA TGGCAGCCCT CCAAAGACGG AGATCGGCTC
    ATCGGCAGGA TCCTGCTCAA CAAGCGCATG AAGGACGGCA GCGTTCCCCG CGATTCCGGA
    GCTCTTCTGG GACTCAAAGT GGTTGGTGGA AAGATGACAG AGTCTGGGCG ACTTTGTGCG
    TTCATTACCA AAGTAAGGAA AGGGAGTTTA GCGGATACAG TTGGGCACCT CAGACCAGGT
    GACCAGGTCC TGGAGTGGAA TGGCCGACAG TTACAAGGAG CCACATTTAA AGAAGTTTAC
    AATATCATTT TAGAATCAAA GCCAGAGCCT CAGGTGGAAC TAGTGGTCTC ACGACCCATA
    GGAGATATAC CCAGGATACC AGAAAGCACA CACGCACAAC TTGAGTCCAG TTCGAGTTCA
    TTTGAGTCTC AGAAGATGGA GCGTCCCTCC ATATCCGTCA CGTCTCCCAT GAGCCCCGGA
    GTGCTGCGGG ACGCCCCTCA GTACTTATCG GGACAGCTTA CAAGCCAAAG CCTTAGTAGA
    AGAATTGAGC CTTTTAACCC TAGAGTTCAG GTCAAACTTT GGTATGACAA AGTGGGCCAT
    CAGCTTATAG TCACCATACT GGGTGCAAAG GATCTGCCTT CAAGGGAAGA CGGTCGACCT
    CGCAATCCCT ATGTCAAGAT CTACTTCCTC CCTGACAGAA GCGACAAAAG CAAAAGAAGA
    ACAAAAACGG TAAAGAAATC ACTAGAGCCC AAATGGAACC AGACCTTTAT GTACTCGCCC
    GTGCACCGGC GGGAGTTCAG GGAGAGAATG CTGGAGATCA CGCTCTGGGA CCAGGCCCGG
    GTTCGGGAGG AGGAGAGCGA GTTCCTCGGG GAGATCCTGA TCGAGCTGGA AACGGCGCTG
    GTGGACGACG AGCCACACTG GTATAAACTG CAGACGCATG ACGTGTCGTC GGTCCCGCTG
    CCCAAGAGCT CCCCCTCCTT GCAGAGACGA GCACTGCATG GGGACAGTCC CACACGCAGA
    CTGCAGACCC AAGGCCCGTT TGTGTACAGC TCAGGGTCTC AGAGGATCAG CGACAGCGAG
    TTCTCAGACT ATGACTGTGA GGATGGCGTT GGCGTCGTAT CAGACTATAG AAACGGCAGG
    GACCTCCAGA GCTCCACCCT CTCTGTGCCT GAGCAAGTCT TATCGTCCAA TCACTGCTCT
    CGGTCTGCTG ATATCAACAG AGCGCGCTCG CGATCTCCCA GCATGCCTCC TTCTCAGAGT
    CATTTTCTCA CGTTACCTCG AACCCGACAC AGTCAGCCTC ATGACAGTGA CCTGCAGGAT
    CTCAGGAATT ACGGAGGAGC CCCGGACCGG CACGAGTACC ACCATCGATC TCGTTCTGCA
    GACCAACGGG CCGCAATGGA GCGTCCCATG TACAGTCGCT CTCGCTCCAC AGAGCGTCCG
    CCCGACGGAC CGCACATGAG GTCCTCCATG CCCTCTCTGC CATCGGGACA CTCTGCACCA
    TCTTCCCCTG CCTTATCGAG GGCTCATCCT CGCGGTGGAT CGGTTCAGAC CAGCCCCACA
    GGGACCCCCA TAAACAGCCG GAGAGGAAGA CAGCTGCCAC AACTGCCTCC AAAGGGGACG
    CTGGAGAGAA AAGATGGAGA AGAAGAAGTT GAGGCTTGCC CAGACTCGGA CTCCCCCAGT
    CGGACAAACT CAGGTGCTCT GGATGTTGAG GAGCGAACGC GGCAGATGAA GCTGAAGATG
    AACAAATATA AACAGGGGAC CGGCTCTGAT TCAAGACTGG AGCAGGACTA TCACAAGCGC
    TCCGGCAGGG ATCCTCAGCG TGGAGAAAAC CTGTCGGCCA AGTCTTCGGA CAGTGATGTG
    AGCGACGTGT CCGCTGTGTC GAGAACCAGC AGTGCATCTC GATTCAGCAG CATGAGCTAC
    ATCTCTGTCC AGTCGGAGCG GCCACGGGGC AGCAGGAAGA TCAGTGAATT TACATCCAAA
    ATGAAAAACA GGCAAATGGG AACGGCGGCT GGAGGCAACA TGACCAAGAG CACCAGCATC
    GGCGGAGACA TGTGCAGCCT GGAGAAGACC GACGGCAGCC AGTCGGACAC GGCCGTAAGC
    CTGGTGGGAA CGGACGACAA GAAACGGCGC TCCAGCATCG GAGCTAAGAT GGCGGCAGTG
    GTGGGATTGA GCCGAAAGAG TCGCAGCGCT TCACAGCTCG GTCAGACAGA AGCAGGAGGG
    AAGAAGCTGC GCAGCACCAT CCAGAGGAGC ACAGAGACGG GACTGGCTGT GGAGATGAGG
    AGCCGAATGA CAAGACAAGC CAGCCGAGAA TCCACTGACG GCAGCATGAA CAGCTACAGC
    TCTGAGGGAA ACCTGATCTT CCCTGGAGTG AGACTCTCTT CAGACAGTCA GTTCAGTGAT
    TTCCTGGATG GACTTGGCCC AGCCCAGCTG GTGGGCAGAC AGACATTAGC CACACCTCCT
    ATGGGTGACA TTCAGATCGG GATGGTGGAA AAGAAAGGAG CCCTAGAGGT GGAGGTGATC
    CGAGCCCGAG GCCTTGTGGG AAAACCAGGT TCAAAGGCAC TGCCAGCCCC TTACGTAAAG
    GTGTATCTAC TGGAGAACGG CGCCTGCATA GCCAAAAAGA AAACAAAAGT AGCAAGAAAA
    ACACTGGATC CTCTTTACCA GCAGCAGCTG TCATTCGAAG AGTCTCCAGG AGGAAAAGTT
    TTACAGATTA TAGTGTGGGG AGATTATGGA CGTATGGACC ACAAATCTTT TATGGGAGCT
    GCTCAGATAC TGTTAGATGA TCTGGACTTG TCCAACATGG TTATTGGCTG GTTTAAGCTG
    TTTCCCCCAT CTTCACTGGT CGACCCAACC TTAGCCCCTT TGACCAGAAG AGCTTCCCAG
    ACATCTCTGG ACAGTGGGTC CGGGCCATTT GCTCGCTCGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149446
    Clone ID Related Accession (Same CDS sequence) XM_009292619.3
    Accession Version XM_009292619.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product regulating synaptic membrane exocytosis protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCGGCTC CAGCCGGGCC CCGCAGCGGG CCTGTCCTGC CGGAGATGCC GGACCTGAGC 
    CACCTCACCG AGGAGGAGAG GAAGATCATC CTCGGTGTCA TGGACAGGCA GAAGAAAGAG
    GAGGAGAAGG AGCAGTCAAT GTTGAAGAAA TTGCACCAGC AGTTTGAAAT GTACAAAGAT
    CAAGTGCAGA AGATGGGTGA GGAGGCTCAG AGCGGCCCGG AGCAGAAGAG CGATGCTCCT
    ACCTGCGGAA TCTGCCATAA AACCAAGTTT GCCGACGGCT GTGGCCACCT CTGTTCTTAT
    TGCCAAACCA AGTTCTGTGC TCGCTGTGGA GGAAGAGTTT CACTGCGCTC TAACAAGGAG
    GATAAAGTGG TGATGTGGGT ATGCAACCTG TGCCGAAAAC AACAAGAAAT CCTCACCAAG
    TCAGGGGCCT GGTTCTTCAG TGGCCCAGGG GGAGCCCTGC CTGCAGGTGC GAGTGACCCT
    CGCCGCCGCC ACGAAGAGGC TCCGCAGGAG AAGAAGGCCA AACTGCAGGA AGCTCCATTG
    CTGTACCAGA GCTCAGGAGA CCGCAGCCGA GCTCCAGGAC TCCCCCGACA GGCATCCCTC
    AACAACAACT CTAGTCTGAA GCCCAACGAC CCAACAAGCA ACAGGAAAAG AAGCCCATCC
    GCAACCAGGG ATCCAAACCA AAAATACAAC CAAAGGGAGG AGAAGGCGGA TCGTTCGCAG
    TATGCACCAG GAGAGGGCGG CATGCCACGA TCGCCGTCTG ACTATGGAGC CGGAGATCGG
    CAGTGGCGGG GAGGCTGCGG GCGCTCCTAT GAGGACGCGG AGGTAGCGCG AGGGGCGTAC
    CGGGCTCGAC GGGGCGGCTG GCACTCTCAG GAGGAATACC CACCCGAACC TGGCTTCCTA
    CCCGATGGAC CACCCCTCAG CGAGCACGAG CTCCAGCGCC AGCGGCAGGA GGAGTACCAG
    AACCGCTACC GCAGTGACCC AAACCTGGCT CGATACCCTG TCAAACCACA GCCCTACGAG
    GAGCAGATGC GCATGCACGC GGAGGTCTCC AGATTTAGGC ATGAACGGCG GCACAGTGAT
    GTCTCCCTGG CGTACACTGA GATGGAAGAG CCCCTGGGGC CTCTGCGGGG TCCACACTAC
    TCACCGGGCT CCGGACAAAC GCACTGGAGG TCCAACCACA GTCCCCCTGG CACAGACTCG
    GCCCACCGGC AGCAGCAGCT CCTGGATCCC GTCTCGGCTG TACGGAAGAG TAAAAGGGAA
    AAGATGGAGA GCATGTTGAG GAACGACTCG CTCAGTTCCG ACCAATCGGA GTCCGTCAGG
    CCGCCGCCAC CCAAACCTCA CAAGACAAAG CGAGGCGGGA AAATGAGGCA GGTTTCACTG
    AGCAGCTCTG AGGAGGAACT GGCCACAACG CCCGAGTACA CCAGCTGCGA GGATGTGGAG
    ATTGAGAGCG AGTCAGTCAG TGAGAAAGGG GACAGTCAAA GGGGAAAAAG ACAAACTTTT
    GAGCAGTCAC ACACCTTATC TGAGGGCCAA AAGAAAATGG TGCGCTTTGG GGGCCACTCT
    TGGGAAGAAG ATGCTGAGTG GTGTGAGCCC CAGGTTAAAG ACTCGGGTGT TGATACGTGC
    AGTAGCACAA CCCTAAACGA GGAGCATAGC CATAGTGAGA AGCACCCAGT TACATGGCAG
    CCCTCCAAAG ACGGAGATCG GCTCATCGGC AGGATCCTGC TCAACAAGCG CATGAAGGAC
    GGCAGCGTTC CCCGCGATTC CGGAGCTCTT CTGGGACTCA AAGTGGTTGG TGGAAAGATG
    ACAGAGTCTG GGCGACTTTG TGCGTTCATT ACCAAAGTAA GGAAAGGGAG TTTAGCGGAT
    ACAGTTGGGC ACCTCAGACC AGGTGACCAG GTCCTGGAGT GGAATGGCCG ACAGTTACAA
    GGAGCCACAT TTAAAGAAGT TTACAATATC ATTTTAGAAT CAAAGCCAGA GCCTCAGGTG
    GAACTAGTGG TCTCACGACC CATAGGAGAT ATACCCAGGA TACCAGAAAG CACACACGCA
    CAACTTGAGT CCAGTTCGAG TTCATTTGAG TCTCAGAAGA TGGAGCGTCC CTCCATATCC
    GTCACGTCTC CCATGAGCCC CGGAGTGCTG CGGGACGCCC CTCAGTACTT ATCGGGACAG
    CTTACAAGCC AAAGCCTTAG TAGAAGAATT GAGCCTTTTA ACCCTAGAGT TCAGGTCAAA
    CTTTGGTATG ACAAAGTGGG CCATCAGCTT ATAGTCACCA TACTGGGTGC AAAGGATCTG
    CCTTCAAGGG AAGACGGTCG ACCTCGCAAT CCCTATGTCA AGATCTACTT CCTCCCTGAC
    AGAAGCGACA AAAGCAAAAG AAGAACAAAA ACGGTAAAGA AATCACTAGA GCCCAAATGG
    AACCAGACCT TTATGTACTC GCCCGTGCAC CGGCGGGAGT TCAGGGAGAG AATGCTGGAG
    ATCACGCTCT GGGACCAGGC CCGGGTTCGG GAGGAGGAGA GCGAGTTCCT CGGGGAGATC
    CTGATCGAGC TGGAAACGGC GCTGGTGGAC GACGAGCCAC ACTGGTATAA ACTGCAGACG
    CATGACGTGT CGTCGGTCCC GCTGCCCAAG AGCTCCCCCT CCTTGCAGAG ACGAGCACTG
    CATGGGGACA GTCCCACACG CAGACTGCAG ACCCAAGGCC CGTTTGTGTA CAGCTCAGGG
    TCTCAGAGGA TCAGCGACAG CGAGTTCTCA GACTATGACT GTGAGGATGG CGTTGGCGTC
    GTATCAGACT ATAGAAACGG CAGGGACCTC CAGAGCTCCA CCCTCTCTGT GCCTGAGCAA
    GTCTTATCGT CCAATCACTG CTCTCGGTCT GCTGATATCA ACAGAGCGCG CTCGCGATCT
    CCCAGCATGC CTCCTTCTCA GAGTCATTTT CTCACGTTAC CTCGAACCCG ACACAGTCAG
    CCTCATGACA GTGACCTGCA GGATCTCAGG AATTACGGAG GAGCCCCGGA CCGGCACGAG
    TACCACCATC GATCTCGTTC TGCAGACCAA CGGGCCGCAA TGGAGCGTCC CATGTACAGT
    CGCTCTCGCT CCACAGAGCG TCCGCCCGAC GGACCGCACA TGAGGTCCTC CATGCCCTCT
    CTGCCATCGG GACACTCTGC ACCATCTTCC CCTGCCTTAT CGAGGGCTCA TCCTCGCGGT
    GGATCGGTTC AGACCAGCCC CACAGGGACC CCCATAAACA GCCGGAGAGG AAGACAGCTG
    CCACAACTGC CTCCAAAGGG GACGCTGGAG AGAAAAGATG GAGAAGAAGA AGTTGAGGCT
    TGCCCAGACT CGGACTCCCC CAGTCGGACA AACTCAGGTG CTCTGGATGT TGAGGAGCGA
    ACGCGGCAGA TGAAGCTGAA GATGAACAAA TATAAACAGG GGACCGGCTC TGATTCAAGA
    CTGGAGCAGG ACTATCACAA GCGCTCCGGC AGGGATCCTC AGCGTGGAGA AAACCTGTCG
    GCCAAGTCTT CGGACAGTGA TGTGAGCGAC GTGTCCGCTG TGTCGAGAAC CAGCAGTGCA
    TCTCGATTCA GCAGCATGAG CTACATCTCT GTCCAGTCGG AGCGGCCACG GGGCAGCAGG
    AAGATCAGGC AAATGGGAAC GGCGGCTGGA GGCAACATGA CCAAGAGCAC CAGCATCGGC
    GGAGACATGT GCAGCCTGGA GAAGACCGAC GGCAGCCAGT CGGACACGGC CGTAAGCCTG
    GTGGGAACGG ACGACAAGAA ACGGCGCTCC AGCATCGGAG CTAAGATGGC GGCAGTGGTG
    GGATTGAGCC GAAAGAGTCG CAGCGCTTCA CAGCTCGGTC AGACAGAAGC AGGAGGGAAG
    AAGCTGCGCA GCACCATCCA GAGGAGCACA GAGACGGGAC TGGCTGTGGA GATGAGGAGC
    CGAATGACAA GACAAGCCAG CCGAGAATCC ACTGACGGCA GCATGAACAG CTACAGCTCT
    GAGGGAAACC TGATCTTCCC TGGAGTGAGA CTCTCTTCAG ACAGTCAGTT CAGTGATTTC
    CTGGATGGAC TTGGCCCAGC CCAGCTGGTG GGCAGACAGA CATTAGCCAC ACCTCCTATG
    GGTGACATTC AGATCGGGAT GGTGGAAAAG AAAGGAGCCC TAGAGGTGGA GGTGATCCGA
    GCCCGAGGCC TTGTGGGAAA ACCAGGTTCA AAGGCACTGC CAGCCCCTTA CGTAAAGGTG
    TATCTACTGG AGAACGGCGC CTGCATAGCC AAAAAGAAAA CAAAAGTAGC AAGAAAAACA
    CTGGATCCTC TTTACCAGCA GCAGCTGTCA TTCGAAGAGT CTCCAGGAGG AAAAGTTTTA
    CAGATTATAG TGTGGGGAGA TTATGGACGT ATGGACCACA AATCTTTTAT GGGAGCTGCT
    CAGATACTGT TAGATGATCT GGACTTGTCC AACATGGTTA TTGGCTGGTT TAAGCTGTTT
    CCCCCATCTT CACTGGTCGA CCCAACCTTA GCCCCTTTGA CCAGAAGAGC TTCCCAGACA
    TCTCTGGACA GTGGGTCCGG GCCATTTGCT CGCTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290894.3
    CDS647..5185
    Translation

    Target ORF information:

    RefSeq Version XM_009292619.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio regulating synaptic membrane exocytosis 2a (rims2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292619.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTCGGCTC CAGCCGGGCC CCGCAGCGGG CCTGTCCTGC CGGAGATGCC GGACCTGAGC 
    CACCTCACCG AGGAGGAGAG GAAGATCATC CTCGGTGTCA TGGACAGGCA GAAGAAAGAG
    GAGGAGAAGG AGCAGTCAAT GTTGAAGAAA TTGCACCAGC AGTTTGAAAT GTACAAAGAT
    CAAGTGCAGA AGATGGGTGA GGAGGCTCAG AGCGGCCCGG AGCAGAAGAG CGATGCTCCT
    ACCTGCGGAA TCTGCCATAA AACCAAGTTT GCCGACGGCT GTGGCCACCT CTGTTCTTAT
    TGCCAAACCA AGTTCTGTGC TCGCTGTGGA GGAAGAGTTT CACTGCGCTC TAACAAGGAG
    GATAAAGTGG TGATGTGGGT ATGCAACCTG TGCCGAAAAC AACAAGAAAT CCTCACCAAG
    TCAGGGGCCT GGTTCTTCAG TGGCCCAGGG GGAGCCCTGC CTGCAGGTGC GAGTGACCCT
    CGCCGCCGCC ACGAAGAGGC TCCGCAGGAG AAGAAGGCCA AACTGCAGGA AGCTCCATTG
    CTGTACCAGA GCTCAGGAGA CCGCAGCCGA GCTCCAGGAC TCCCCCGACA GGCATCCCTC
    AACAACAACT CTAGTCTGAA GCCCAACGAC CCAACAAGCA ACAGGAAAAG AAGCCCATCC
    GCAACCAGGG ATCCAAACCA AAAATACAAC CAAAGGGAGG AGAAGGCGGA TCGTTCGCAG
    TATGCACCAG GAGAGGGCGG CATGCCACGA TCGCCGTCTG ACTATGGAGC CGGAGATCGG
    CAGTGGCGGG GAGGCTGCGG GCGCTCCTAT GAGGACGCGG AGGTAGCGCG AGGGGCGTAC
    CGGGCTCGAC GGGGCGGCTG GCACTCTCAG GAGGAATACC CACCCGAACC TGGCTTCCTA
    CCCGATGGAC CACCCCTCAG CGAGCACGAG CTCCAGCGCC AGCGGCAGGA GGAGTACCAG
    AACCGCTACC GCAGTGACCC AAACCTGGCT CGATACCCTG TCAAACCACA GCCCTACGAG
    GAGCAGATGC GCATGCACGC GGAGGTCTCC AGATTTAGGC ATGAACGGCG GCACAGTGAT
    GTCTCCCTGG CGTACACTGA GATGGAAGAG CCCCTGGGGC CTCTGCGGGG TCCACACTAC
    TCACCGGGCT CCGGACAAAC GCACTGGAGG TCCAACCACA GTCCCCCTGG CACAGACTCG
    GCCCACCGGC AGCAGCAGCT CCTGGATCCC GTCTCGGCTG TACGGAAGAG TAAAAGGGAA
    AAGATGGAGA GCATGTTGAG GAACGACTCG CTCAGTTCCG ACCAATCGGA GTCCGTCAGG
    CCGCCGCCAC CCAAACCTCA CAAGACAAAG CGAGGCGGGA AAATGAGGCA GGTTTCACTG
    AGCAGCTCTG AGGAGGAACT GGCCACAACG CCCGAGTACA CCAGCTGCGA GGATGTGGAG
    ATTGAGAGCG AGTCAGTCAG TGAGAAAGGG GACAGTCAAA GGGGAAAAAG ACAAACTTTT
    GAGCAGTCAC ACACCTTATC TGAGGGCCAA AAGAAAATGG TGCGCTTTGG GGGCCACTCT
    TGGGAAGAAG ATGCTGAGTG GTGTGAGCCC CAGGTTAAAG ACTCGGGTGT TGATACGTGC
    AGTAGCACAA CCCTAAACGA GGAGCATAGC CATAGTGAGA AGCACCCAGT TACATGGCAG
    CCCTCCAAAG ACGGAGATCG GCTCATCGGC AGGATCCTGC TCAACAAGCG CATGAAGGAC
    GGCAGCGTTC CCCGCGATTC CGGAGCTCTT CTGGGACTCA AAGTGGTTGG TGGAAAGATG
    ACAGAGTCTG GGCGACTTTG TGCGTTCATT ACCAAAGTAA GGAAAGGGAG TTTAGCGGAT
    ACAGTTGGGC ACCTCAGACC AGGTGACCAG GTCCTGGAGT GGAATGGCCG ACAGTTACAA
    GGAGCCACAT TTAAAGAAGT TTACAATATC ATTTTAGAAT CAAAGCCAGA GCCTCAGGTG
    GAACTAGTGG TCTCACGACC CATAGGAGAT ATACCCAGGA TACCAGAAAG CACACACGCA
    CAACTTGAGT CCAGTTCGAG TTCATTTGAG TCTCAGAAGA TGGAGCGTCC CTCCATATCC
    GTCACGTCTC CCATGAGCCC CGGAGTGCTG CGGGACGCCC CTCAGTACTT ATCGGGACAG
    CTTACAAGCC AAAGCCTTAG TAGAAGAATT GAGCCTTTTA ACCCTAGAGT TCAGGTCAAA
    CTTTGGTATG ACAAAGTGGG CCATCAGCTT ATAGTCACCA TACTGGGTGC AAAGGATCTG
    CCTTCAAGGG AAGACGGTCG ACCTCGCAAT CCCTATGTCA AGATCTACTT CCTCCCTGAC
    AGAAGCGACA AAAGCAAAAG AAGAACAAAA ACGGTAAAGA AATCACTAGA GCCCAAATGG
    AACCAGACCT TTATGTACTC GCCCGTGCAC CGGCGGGAGT TCAGGGAGAG AATGCTGGAG
    ATCACGCTCT GGGACCAGGC CCGGGTTCGG GAGGAGGAGA GCGAGTTCCT CGGGGAGATC
    CTGATCGAGC TGGAAACGGC GCTGGTGGAC GACGAGCCAC ACTGGTATAA ACTGCAGACG
    CATGACGTGT CGTCGGTCCC GCTGCCCAAG AGCTCCCCCT CCTTGCAGAG ACGAGCACTG
    CATGGGGACA GTCCCACACG CAGACTGCAG ACCCAAGGCC CGTTTGTGTA CAGCTCAGGG
    TCTCAGAGGA TCAGCGACAG CGAGTTCTCA GACTATGACT GTGAGGATGG CGTTGGCGTC
    GTATCAGACT ATAGAAACGG CAGGGACCTC CAGAGCTCCA CCCTCTCTGT GCCTGAGCAA
    GTCTTATCGT CCAATCACTG CTCTCGGTCT GCTGATATCA ACAGAGCGCG CTCGCGATCT
    CCCAGCATGC CTCCTTCTCA GAGTCATTTT CTCACGTTAC CTCGAACCCG ACACAGTCAG
    CCTCATGACA GTGACCTGCA GGATCTCAGG AATTACGGAG GAGCCCCGGA CCGGCACGAG
    TACCACCATC GATCTCGTTC TGCAGACCAA CGGGCCGCAA TGGAGCGTCC CATGTACAGT
    CGCTCTCGCT CCACAGAGCG TCCGCCCGAC GGACCGCACA TGAGGTCCTC CATGCCCTCT
    CTGCCATCGG GACACTCTGC ACCATCTTCC CCTGCCTTAT CGAGGGCTCA TCCTCGCGGT
    GGATCGGTTC AGACCAGCCC CACAGGGACC CCCATAAACA GCCGGAGAGG AAGACAGCTG
    CCACAACTGC CTCCAAAGGG GACGCTGGAG AGAAAAGATG GAGAAGAAGA AGTTGAGGCT
    TGCCCAGACT CGGACTCCCC CAGTCGGACA AACTCAGGTG CTCTGGATGT TGAGGAGCGA
    ACGCGGCAGA TGAAGCTGAA GATGAACAAA TATAAACAGG GGACCGGCTC TGATTCAAGA
    CTGGAGCAGG ACTATCACAA GCGCTCCGGC AGGGATCCTC AGCGTGGAGA AAACCTGTCG
    GCCAAGTCTT CGGACAGTGA TGTGAGCGAC GTGTCCGCTG TGTCGAGAAC CAGCAGTGCA
    TCTCGATTCA GCAGCATGAG CTACATCTCT GTCCAGTCGG AGCGGCCACG GGGCAGCAGG
    AAGATCAGGC AAATGGGAAC GGCGGCTGGA GGCAACATGA CCAAGAGCAC CAGCATCGGC
    GGAGACATGT GCAGCCTGGA GAAGACCGAC GGCAGCCAGT CGGACACGGC CGTAAGCCTG
    GTGGGAACGG ACGACAAGAA ACGGCGCTCC AGCATCGGAG CTAAGATGGC GGCAGTGGTG
    GGATTGAGCC GAAAGAGTCG CAGCGCTTCA CAGCTCGGTC AGACAGAAGC AGGAGGGAAG
    AAGCTGCGCA GCACCATCCA GAGGAGCACA GAGACGGGAC TGGCTGTGGA GATGAGGAGC
    CGAATGACAA GACAAGCCAG CCGAGAATCC ACTGACGGCA GCATGAACAG CTACAGCTCT
    GAGGGAAACC TGATCTTCCC TGGAGTGAGA CTCTCTTCAG ACAGTCAGTT CAGTGATTTC
    CTGGATGGAC TTGGCCCAGC CCAGCTGGTG GGCAGACAGA CATTAGCCAC ACCTCCTATG
    GGTGACATTC AGATCGGGAT GGTGGAAAAG AAAGGAGCCC TAGAGGTGGA GGTGATCCGA
    GCCCGAGGCC TTGTGGGAAA ACCAGGTTCA AAGGCACTGC CAGCCCCTTA CGTAAAGGTG
    TATCTACTGG AGAACGGCGC CTGCATAGCC AAAAAGAAAA CAAAAGTAGC AAGAAAAACA
    CTGGATCCTC TTTACCAGCA GCAGCTGTCA TTCGAAGAGT CTCCAGGAGG AAAAGTTTTA
    CAGATTATAG TGTGGGGAGA TTATGGACGT ATGGACCACA AATCTTTTAT GGGAGCTGCT
    CAGATACTGT TAGATGATCT GGACTTGTCC AACATGGTTA TTGGCTGGTT TAAGCTGTTT
    CCCCCATCTT CACTGGTCGA CCCAACCTTA GCCCCTTTGA CCAGAAGAGC TTCCCAGACA
    TCTCTGGACA GTGGGTCCGG GCCATTTGCT CGCTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.