tjp3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tjp3
Entrez Gene ID	373089
Full Name	tight junction protein 3
Synonyms	cb766,wu:fj93c08
General protein information	Preferred Names tight junction protein 3 Names tight junction protein ZO-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328340.1	NP_001315269.1	tight junction protein ZO-3
XM_005170442.3	XP_005170499.1	tight junction protein ZO-3 isoform X1
XM_005170442.4	XP_005170499.1	tight junction protein ZO-3 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Rapid reverse genetic screening using CRISPR in zebrafish.
Shah AN, Davey CF, Whitebirch AC, Miller AC, Moens CB
Nature methods12(6)535-40(2015 Jun)

Temporal and spatial expression of tight junction genes during zebrafish pronephros development.
McKee R, Gerlach GF, Jou J, Cheng CN, Wingert RA
Gene expression patterns : GEP16(2)104-13(2014 Nov)

Unilateral dampening of Bmp activity by nodal generates cardiac left-right asymmetry.
Veerkamp J, Rudolph F, Cseresnyes Z, Priller F, Otten C, Renz M, Schaefer L, Abdelilah-Seyfried S
Developmental cell24(6)660-7(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tjp3/Zo-3 is critical for barrier function of the enveloping cell layer and osmoregulation in early stages of zebrafish development.
Title: Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos.

analysis of tissue distribution and developmental expression of tjp1/zo-1, tjp2/zo-2 and tjp3/zo-3 in the zebrafish, Danio rerio
Title: Identification, tissue distribution and developmental expression of tjp1/zo-1, tjp2/zo-2 and tjp3/zo-3 in the zebrafish, Danio rerio.

The following tjp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tjp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26853	NM_001328340.1 Latest version!	Danio rerio tight junction protein 3 (tjp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa26853	XM_005170442.4 Latest version!	Danio rerio tight junction protein 3 (tjp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa26853	XM_005170442.3	Danio rerio tight junction protein 3 (tjp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tjp3 ( NM_001328340.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315269.1 Danio rerio tight junction protein 3 (tjp3), mRNA.

Price & Availability↑

CloneID	ODa26853
Clone ID Related Accession (Same CDS sequence)	NM_001328340.1 , XM_005170442.3 , XM_005170442.4
Accession Version	NM_001328340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU856138.6 and CU695074.11. On Jun 12, 2016 this sequence version replaced XM_005170441.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01011759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA TGACGATATG GGAGCAGCAC ACGGTCACGT TATCGAAAGA TTCCAAGGTG 
GGCTTTGGTT TTGCCGTCTC AGGTGGGCTG GACAAACCCA ACCCAGCAAA TGGGGACGCG 
GCAGTGGTGG TCTCTGATGT GATTCCCACC GGTCCCGCCA TGGGCCGACT TCAGACGCGA 
GATCAGATTG TTATGGTGAA TGGTGTTTCA ATGGAGAACG TCACTTCACT CTACACCATA 
CAGAATCTGA GGAAGTGCGG GAAGACCGCT AACATTACTG TGAAGAGGCC GCGCACGATC 
CAGATTCCTG CCAGCAGCAG ACCCACTCGC TCAGCCTCTC AGTCCAACAT TTTAGACGAT 
AAAGGACCTC AGTATAAGGG TCAGCGGGGC CCTGACACCA CACGCACCCG CCGGGCCCGA 
AGCGTCACTC CTGACCGCAA TGGCCACGAT CTGCCCATCA CATCCGGATA CAAGCGACTG 
CCCAATCAGG ACGTCCAGAA CAAACCCATC AAGACCACTC TGATCAAGAA GAAGCCCACC 
GACGAGTATG GAATGAAGCT GGGGAGCCAG ATCTTCATCA AGCACATGAC TCCCACCGGT 
CTGGCTGCCA AAGAAGGCTC GCTTCAGGAG GGAGATCTGG TTTTAAAGAT TAACGGCATG 
ACAACAGAAA ACCTGTCCCT GCTGGAGACC AAGCATCTGG TGGAGAAATC CAGAGGGAAC 
CTGACCATGC TGGTGCTGAG AGACGACAGA AAGTTTCTGG TCAGCATCCC TGAAGTGGAG 
GACAGTCCTC AAAACAGCTC AGACGAGAGG CGGGAGGACA GCAGCTCTGA GCTGGAGGAA 
ATCTCAGACC TGGATTCGGA CCTTCCCGTT ACAAACCGAG CATCTCGTCG CAAAGACCGA 
AAATCGAACA GAAACCGGGC TGATCCTGTG TCGAAAAAAC TTGAATCCCC TCCTCTGCGC 
TCAACTCTCA CAAGACCTGC TCAGTCTATG CCTCCTCGCA GAGTTCCCTC GGAGTCTGAA 
TCTGATCGCA GCAACTCTCC TCCACCAAGA CATGACACTC TCGATCACAG AAACAAGGTT 
CTCTCTGGGT ACTCCACACT GCCAAACCCT CGAGCTTCAC CCATCACCTC CTCCTCCACA 
TCCCGGCCTC GCAGAGCCGT TTCTGAGTCA GATTCAGACC GGAGCGCATC TCCACCGCCC 
AGACGTGAGA GTCCCAGACC TGATAACCAC TCCAGATACA AGGTGCTTCC TGATCTCCCC 
CCTCCAGGTT TGAAGCCTTC TTCTCTTAGC ATGTCTCAGG ATTTCCCTCG CCGCATGGCT 
TCACCTTACA GGCCTCCACC TAGAGATGCT TCAGAGTCTG AATCGGACGA AAGTGCCAGA 
CCTCAGCGCA GACAGGGGAC TCCGGCCAGC CAAGAGTCCC GCAATAGATA CAGGGCTGCC 
CCTGAAGTGA AGGCAGCAGC AGTAATGGAG CCCCCAAAAT GGGCGAAACC CTCAACGCCT 
GTACAGAGAG CTCCCTCAGA ATCTGAATCA GATGGAAGTT ACAACTCTCC ACCCAAACAA 
CCTCCGTCTC CAGTCAGTAA AAGCTCCAGA ATCAAAGGGC CTGAAATGCA AATGCCAATC 
ACCAGCATCC GGCAGGATCC TCCACTCAGA GCCGCTCCTG CCAGACCACC TCCCGAAGAT 
TCCTCTGACT CAGAAAGAGC CCAGTCACCT CTCAGGAGAT CTGACAGTCA CAACAGCGAT 
CACAGTTTCC CACGTGCTAA TGGGACTCTC CGCTCGAACG TCTCCATCCC CAACCACACT 
GCGCTGACTT CTAAAGCTGT AGAAGAACCA CTTTATGTGC TTCCACCAGA CGCAGTGCCA 
ACACCAAACC TGGGGTACAG CTCCGATCAG AACCATATCA GCTTTGTGAA GGAAGGCAGT 
GTCGGCTTGA GACTTGTGGG AGGAAATGAT GTTGGCATCT TTGTCGGTGG TGTCCAGCCT 
AACAGTCCAG CCCAAAAGCA AGGCATAACA GAGGGTGATC AGATTCTGGA GGTAAATGGC 
ATGGATTTTA ACCACTTTAC CAGAGAAGAA GCAGCAATGT ATCTTATGAA CATACGTGGT 
GGAGAGCGTA TCGATATGCG GACTCAGAAC AAAGCAGACA TCTACAAGAA GATTCTGAAG 
TCCAACCTTG GAGATTCTTT CTACATCCGC ACACACTATG ATCATGTGGC AGACAGCAGC 
GTCGGCTTAA GCTTTACCAG AGGCGAGGTG TTCAGAGTGG TGGACACTAT GCATCGAGGA 
AAGCTGGGGA CCTGGTTAGC TGTGCGCATG GGCAATGATC TACACGAACT GGACAAAGGG 
ACCATTCCTA ACCAGACCAG GGCTGAAACT CTGGCTAATT TGGAGCAGGC GCAGAGAATT 
AGTGCAGCTG AACGTCAGGC ATCTGGCCCC AGAGCTGAAT TCTGGAAGCT ACGGGGTCTC 
AGAGGGGCCA AAAAGAGTAA CCGAAGAACC CGTGATGACC TCCTGCAGTT GACAATTCAA 
GGCAAATTTC CAGCATACGA GAAGGTCCTG TTGAAAGAAG CCAATTTCAA ACGTCCTATT 
GTCATCTTGG GTCCTCTGAA TGACATTGCC AATGAAAAAC TGGCCCGAGA GTTGCCTGAT 
GAATTTGAAA TTGCAGAAAT GGTACCCAGG AGTGGAAATG ATAGTTCATC AAGTGTAATC 
AAACTGGACA CCGTAAGGCG GATAGCGGAG CAGGACAAGC ATCCCTTGCT GGACATTACA 
CCCACTGCCA TAGAAAGATT GAATTATATC CAGTACCATC CCATGGTGCT CTTCTTGGAT 
CCTCATAGTC GGAAGGACGT GAAGTCTATG AGGCAAAAAT ACATGCCAGG CTCCAGCAAG 
AGCTCTCGAC GCCTCTATGC CCAGGCCTTA AAGCTAAGGA AAAACTACAG CACTCTGTTT 
TCTGCACGCA TTGATCTTCA GCCAAATACA AACATGTGGT ATGATGTTTT GAAAGACAAG 
ATTCGGCAAC AGCAGTCTAA GCCTGTCTGG GTTTCTGAAG TAGCGATGGA AGGAGGAGCC 
GAAGAGGATC TTGACGCATT TAATCGAGCA AACAACTCTG ACTATCTCAG CGGTGCCAGT 
GACTACGAGG ACACTGACGG TGAGGCCTTC ACAGACGGTG AAGCCTACAC GGACAATGAA 
GACCTGGAGG AACCCTTAGG AGGCATTGCC AAACACCGCA GCTCTGCGCT GGCCCGTTCT 
TCGGAGCCTG CCAGTGTGTT AAATCATAGC CCTGAACCTC CCTACAACCT ACACGAGGAG 
GAGGAAGAGC CAGAGTACAC CCTACCGCCC AGTGAAGTTC CTCCCATCCT CCATGTTCCC 
GAGCCCCGAT CTCCACGCCT TGTTGCTGGA ATACCAGAAG AGCAAGAGGA TGTGCCCTCG 
CAGCGCAGTT TCACAGATTC AGACTTCAGC AATGATGATG TTGTTGCACC TCAAACACCT 
TCCGATGCGC CACCCAAATT CAGGGCACCA GATCCCAAAG ATGTCCAGTC AAAGACCCCT 
CCACTGTCAG CCATAGAGGA GAGACTACAA CAGGCTCGCA TGGCAGAACA GATTGAACCA 
GAAAGGAAAC CTTCGCCTTC TTTTATAGTA CTCGCACATC ATGAAGCCAT GCAAATGCGG 
AGAACGCAAA TCCGAGGAAG CGACAGTGAC AGCGACGAAA ACGACAGAGA TGAAGACGAA 
TATGACGAAG CAGAAGACAT TGAATGGGGA CCTGCTACAG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315269.1
CDS	191..3958
Translation	MEEMTIWEQHTVTLSKDSKVGFGFAVSGGLDKPNPANGDAAVVVSDVIPTGPAMGRLQTRDQIVMVNGVSMENVTSLYTIQNLRKCGKTANITVKRPRTIQIPASSRPTRSASQSNILDDKGPQYKGQRGPDTTRTRRARSVTPDRNGHDLPITSGYKRLPNQDVQNKPIKTTLIKKKPTDEYGMKLGSQIFIKHMTPTGLAAKEGSLQEGDLVLKINGMTTENLSLLETKHLVEKSRGNLTMLVLRDDRKFLVSIPEVEDSPQNSSDERREDSSSELEEISDLDSDLPVTNRASRRKDRKSNRNRADPVSKKLESPPLRSTLTRPAQSMPPRRVPSESESDRSNSPPPRHDTLDHRNKVLSGYSTLPNPRASPITSSSTSRPRRAVSESDSDRSASPPPRRESPRPDNHSRYKVLPDLPPPGLKPSSLSMSQDFPRRMASPYRPPPRDASESESDESARPQRRQGTPASQESRNRYRAAPEVKAAAVMEPPKWAKPSTPVQRAPSESESDGSYNSPPKQPPSPVSKSSRIKGPEMQMPITSIRQDPPLRAAPARPPPEDSSDSERAQSPLRRSDSHNSDHSFPRANGTLRSNVSIPNHTALTSKAVEEPLYVLPPDAVPTPNLGYSSDQNHISFVKEGSVGLRLVGGNDVGIFVGGVQPNSPAQKQGITEGDQILEVNGMDFNHFTREEAAMYLMNIRGGERIDMRTQNKADIYKKILKSNLGDSFYIRTHYDHVADSSVGLSFTRGEVFRVVDTMHRGKLGTWLAVRMGNDLHELDKGTIPNQTRAETLANLEQAQRISAAERQASGPRAEFWKLRGLRGAKKSNRRTRDDLLQLTIQGKFPAYEKVLLKEANFKRPIVILGPLNDIANEKLARELPDEFEIAEMVPRSGNDSSSSVIKLDTVRRIAEQDKHPLLDITPTAIERLNYIQYHPMVLFLDPHSRKDVKSMRQKYMPGSSKSSRRLYAQALKLRKNYSTLFSARIDLQPNTNMWYDVLKDKIRQQQSKPVWVSEVAMEGGAEEDLDAFNRANNSDYLSGASDYEDTDGEAFTDGEAYTDNEDLEEPLGGIAKHRSSALARSSEPASVLNHSPEPPYNLHEEEEEPEYTLPPSEVPPILHVPEPRSPRLVAGIPEEQEDVPSQRSFTDSDFSNDDVVAPQTPSDAPPKFRAPDPKDVQSKTPPLSAIEERLQQARMAEQIEPERKPSPSFIVLAHHEAMQMRRTQIRGSDSDSDENDRDEDEYDEAEDIEWGPATEL

Target ORF information:

RefSeq Version	NM_001328340.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 3 (tjp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328340.1

ORF Insert Sequence:

ATGGAGGAGA TGACGATATG GGAGCAGCAC ACGGTCACGT TATCGAAAGA TTCCAAGGTG 
GGCTTTGGTT TTGCCGTCTC AGGTGGGCTG GACAAACCCA ACCCAGCAAA TGGGGACGCG 
GCAGTGGTGG TCTCTGATGT GATTCCCACC GGTCCCGCCA TGGGCCGACT TCAGACGCGA 
GATCAGATTG TTATGGTGAA TGGTGTTTCA ATGGAGAACG TCACTTCACT CTACACCATA 
CAGAATCTGA GGAAGTGCGG GAAGACCGCT AACATTACTG TGAAGAGGCC GCGCACGATC 
CAGATTCCTG CCAGCAGCAG ACCCACTCGC TCAGCCTCTC AGTCCAACAT TTTAGACGAT 
AAAGGACCTC AGTATAAGGG TCAGCGGGGC CCTGACACCA CACGCACCCG CCGGGCCCGA 
AGCGTCACTC CTGACCGCAA TGGCCACGAT CTGCCCATCA CATCCGGATA CAAGCGACTG 
CCCAATCAGG ACGTCCAGAA CAAACCCATC AAGACCACTC TGATCAAGAA GAAGCCCACC 
GACGAGTATG GAATGAAGCT GGGGAGCCAG ATCTTCATCA AGCACATGAC TCCCACCGGT 
CTGGCTGCCA AAGAAGGCTC GCTTCAGGAG GGAGATCTGG TTTTAAAGAT TAACGGCATG 
ACAACAGAAA ACCTGTCCCT GCTGGAGACC AAGCATCTGG TGGAGAAATC CAGAGGGAAC 
CTGACCATGC TGGTGCTGAG AGACGACAGA AAGTTTCTGG TCAGCATCCC TGAAGTGGAG 
GACAGTCCTC AAAACAGCTC AGACGAGAGG CGGGAGGACA GCAGCTCTGA GCTGGAGGAA 
ATCTCAGACC TGGATTCGGA CCTTCCCGTT ACAAACCGAG CATCTCGTCG CAAAGACCGA 
AAATCGAACA GAAACCGGGC TGATCCTGTG TCGAAAAAAC TTGAATCCCC TCCTCTGCGC 
TCAACTCTCA CAAGACCTGC TCAGTCTATG CCTCCTCGCA GAGTTCCCTC GGAGTCTGAA 
TCTGATCGCA GCAACTCTCC TCCACCAAGA CATGACACTC TCGATCACAG AAACAAGGTT 
CTCTCTGGGT ACTCCACACT GCCAAACCCT CGAGCTTCAC CCATCACCTC CTCCTCCACA 
TCCCGGCCTC GCAGAGCCGT TTCTGAGTCA GATTCAGACC GGAGCGCATC TCCACCGCCC 
AGACGTGAGA GTCCCAGACC TGATAACCAC TCCAGATACA AGGTGCTTCC TGATCTCCCC 
CCTCCAGGTT TGAAGCCTTC TTCTCTTAGC ATGTCTCAGG ATTTCCCTCG CCGCATGGCT 
TCACCTTACA GGCCTCCACC TAGAGATGCT TCAGAGTCTG AATCGGACGA AAGTGCCAGA 
CCTCAGCGCA GACAGGGGAC TCCGGCCAGC CAAGAGTCCC GCAATAGATA CAGGGCTGCC 
CCTGAAGTGA AGGCAGCAGC AGTAATGGAG CCCCCAAAAT GGGCGAAACC CTCAACGCCT 
GTACAGAGAG CTCCCTCAGA ATCTGAATCA GATGGAAGTT ACAACTCTCC ACCCAAACAA 
CCTCCGTCTC CAGTCAGTAA AAGCTCCAGA ATCAAAGGGC CTGAAATGCA AATGCCAATC 
ACCAGCATCC GGCAGGATCC TCCACTCAGA GCCGCTCCTG CCAGACCACC TCCCGAAGAT 
TCCTCTGACT CAGAAAGAGC CCAGTCACCT CTCAGGAGAT CTGACAGTCA CAACAGCGAT 
CACAGTTTCC CACGTGCTAA TGGGACTCTC CGCTCGAACG TCTCCATCCC CAACCACACT 
GCGCTGACTT CTAAAGCTGT AGAAGAACCA CTTTATGTGC TTCCACCAGA CGCAGTGCCA 
ACACCAAACC TGGGGTACAG CTCCGATCAG AACCATATCA GCTTTGTGAA GGAAGGCAGT 
GTCGGCTTGA GACTTGTGGG AGGAAATGAT GTTGGCATCT TTGTCGGTGG TGTCCAGCCT 
AACAGTCCAG CCCAAAAGCA AGGCATAACA GAGGGTGATC AGATTCTGGA GGTAAATGGC 
ATGGATTTTA ACCACTTTAC CAGAGAAGAA GCAGCAATGT ATCTTATGAA CATACGTGGT 
GGAGAGCGTA TCGATATGCG GACTCAGAAC AAAGCAGACA TCTACAAGAA GATTCTGAAG 
TCCAACCTTG GAGATTCTTT CTACATCCGC ACACACTATG ATCATGTGGC AGACAGCAGC 
GTCGGCTTAA GCTTTACCAG AGGCGAGGTG TTCAGAGTGG TGGACACTAT GCATCGAGGA 
AAGCTGGGGA CCTGGTTAGC TGTGCGCATG GGCAATGATC TACACGAACT GGACAAAGGG 
ACCATTCCTA ACCAGACCAG GGCTGAAACT CTGGCTAATT TGGAGCAGGC GCAGAGAATT 
AGTGCAGCTG AACGTCAGGC ATCTGGCCCC AGAGCTGAAT TCTGGAAGCT ACGGGGTCTC 
AGAGGGGCCA AAAAGAGTAA CCGAAGAACC CGTGATGACC TCCTGCAGTT GACAATTCAA 
GGCAAATTTC CAGCATACGA GAAGGTCCTG TTGAAAGAAG CCAATTTCAA ACGTCCTATT 
GTCATCTTGG GTCCTCTGAA TGACATTGCC AATGAAAAAC TGGCCCGAGA GTTGCCTGAT 
GAATTTGAAA TTGCAGAAAT GGTACCCAGG AGTGGAAATG ATAGTTCATC AAGTGTAATC 
AAACTGGACA CCGTAAGGCG GATAGCGGAG CAGGACAAGC ATCCCTTGCT GGACATTACA 
CCCACTGCCA TAGAAAGATT GAATTATATC CAGTACCATC CCATGGTGCT CTTCTTGGAT 
CCTCATAGTC GGAAGGACGT GAAGTCTATG AGGCAAAAAT ACATGCCAGG CTCCAGCAAG 
AGCTCTCGAC GCCTCTATGC CCAGGCCTTA AAGCTAAGGA AAAACTACAG CACTCTGTTT 
TCTGCACGCA TTGATCTTCA GCCAAATACA AACATGTGGT ATGATGTTTT GAAAGACAAG 
ATTCGGCAAC AGCAGTCTAA GCCTGTCTGG GTTTCTGAAG TAGCGATGGA AGGAGGAGCC 
GAAGAGGATC TTGACGCATT TAATCGAGCA AACAACTCTG ACTATCTCAG CGGTGCCAGT 
GACTACGAGG ACACTGACGG TGAGGCCTTC ACAGACGGTG AAGCCTACAC GGACAATGAA 
GACCTGGAGG AACCCTTAGG AGGCATTGCC AAACACCGCA GCTCTGCGCT GGCCCGTTCT 
TCGGAGCCTG CCAGTGTGTT AAATCATAGC CCTGAACCTC CCTACAACCT ACACGAGGAG 
GAGGAAGAGC CAGAGTACAC CCTACCGCCC AGTGAAGTTC CTCCCATCCT CCATGTTCCC 
GAGCCCCGAT CTCCACGCCT TGTTGCTGGA ATACCAGAAG AGCAAGAGGA TGTGCCCTCG 
CAGCGCAGTT TCACAGATTC AGACTTCAGC AATGATGATG TTGTTGCACC TCAAACACCT 
TCCGATGCGC CACCCAAATT CAGGGCACCA GATCCCAAAG ATGTCCAGTC AAAGACCCCT 
CCACTGTCAG CCATAGAGGA GAGACTACAA CAGGCTCGCA TGGCAGAACA GATTGAACCA 
GAAAGGAAAC CTTCGCCTTC TTTTATAGTA CTCGCACATC ATGAAGCCAT GCAAATGCGG 
AGAACGCAAA TCCGAGGAAG CGACAGTGAC AGCGACGAAA ACGACAGAGA TGAAGACGAA 
TATGACGAAG CAGAAGACAT TGAATGGGGA CCTGCTACAG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_005170442.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170499.1 Danio rerio tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26853
Clone ID Related Accession (Same CDS sequence)	NM_001328340.1 , XM_005170442.3 , XM_005170442.4
Accession Version	XM_005170442.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction protein ZO-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA TGACGATATG GGAGCAGCAC ACGGTCACGT TATCGAAAGA TTCCAAGGTG 
GGCTTTGGTT TTGCCGTCTC AGGTGGGCTG GACAAACCCA ACCCAGCAAA TGGGGACGCG 
GCAGTGGTGG TCTCTGATGT GATTCCCACC GGTCCCGCCA TGGGCCGACT TCAGACGCGA 
GATCAGATTG TTATGGTGAA TGGTGTTTCA ATGGAGAACG TCACTTCACT CTACACCATA 
CAGAATCTGA GGAAGTGCGG GAAGACCGCT AACATTACTG TGAAGAGGCC GCGCACGATC 
CAGATTCCTG CCAGCAGCAG ACCCACTCGC TCAGCCTCTC AGTCCAACAT TTTAGACGAT 
AAAGGACCTC AGTATAAGGG TCAGCGGGGC CCTGACACCA CACGCACCCG CCGGGCCCGA 
AGCGTCACTC CTGACCGCAA TGGCCACGAT CTGCCCATCA CATCCGGATA CAAGCGACTG 
CCCAATCAGG ACGTCCAGAA CAAACCCATC AAGACCACTC TGATCAAGAA GAAGCCCACC 
GACGAGTATG GAATGAAGCT GGGGAGCCAG ATCTTCATCA AGCACATGAC TCCCACCGGT 
CTGGCTGCCA AAGAAGGCTC GCTTCAGGAG GGAGATCTGG TTTTAAAGAT TAACGGCATG 
ACAACAGAAA ACCTGTCCCT GCTGGAGACC AAGCATCTGG TGGAGAAATC CAGAGGGAAC 
CTGACCATGC TGGTGCTGAG AGACGACAGA AAGTTTCTGG TCAGCATCCC TGAAGTGGAG 
GACAGTCCTC AAAACAGCTC AGACGAGAGG CGGGAGGACA GCAGCTCTGA GCTGGAGGAA 
ATCTCAGACC TGGATTCGGA CCTTCCCGTT ACAAACCGAG CATCTCGTCG CAAAGACCGA 
AAATCGAACA GAAACCGGGC TGATCCTGTG TCGAAAAAAC TTGAATCCCC TCCTCTGCGC 
TCAACTCTCA CAAGACCTGC TCAGTCTATG CCTCCTCGCA GAGTTCCCTC GGAGTCTGAA 
TCTGATCGCA GCAACTCTCC TCCACCAAGA CATGACACTC TCGATCACAG AAACAAGGTT 
CTCTCTGGGT ACTCCACACT GCCAAACCCT CGAGCTTCAC CCATCACCTC CTCCTCCACA 
TCCCGGCCTC GCAGAGCCGT TTCTGAGTCA GATTCAGACC GGAGCGCATC TCCACCGCCC 
AGACGTGAGA GTCCCAGACC TGATAACCAC TCCAGATACA AGGTGCTTCC TGATCTCCCC 
CCTCCAGGTT TGAAGCCTTC TTCTCTTAGC ATGTCTCAGG ATTTCCCTCG CCGCATGGCT 
TCACCTTACA GGCCTCCACC TAGAGATGCT TCAGAGTCTG AATCGGACGA AAGTGCCAGA 
CCTCAGCGCA GACAGGGGAC TCCGGCCAGC CAAGAGTCCC GCAATAGATA CAGGGCTGCC 
CCTGAAGTGA AGGCAGCAGC AGTAATGGAG CCCCCAAAAT GGGCGAAACC CTCAACGCCT 
GTACAGAGAG CTCCCTCAGA ATCTGAATCA GATGGAAGTT ACAACTCTCC ACCCAAACAA 
CCTCCGTCTC CAGTCAGTAA AAGCTCCAGA ATCAAAGGGC CTGAAATGCA AATGCCAATC 
ACCAGCATCC GGCAGGATCC TCCACTCAGA GCCGCTCCTG CCAGACCACC TCCCGAAGAT 
TCCTCTGACT CAGAAAGAGC CCAGTCACCT CTCAGGAGAT CTGACAGTCA CAACAGCGAT 
CACAGTTTCC CACGTGCTAA TGGGACTCTC CGCTCGAACG TCTCCATCCC CAACCACACT 
GCGCTGACTT CTAAAGCTGT AGAAGAACCA CTTTATGTGC TTCCACCAGA CGCAGTGCCA 
ACACCAAACC TGGGGTACAG CTCCGATCAG AACCATATCA GCTTTGTGAA GGAAGGCAGT 
GTCGGCTTGA GACTTGTGGG AGGAAATGAT GTTGGCATCT TTGTCGGTGG TGTCCAGCCT 
AACAGTCCAG CCCAAAAGCA AGGCATAACA GAGGGTGATC AGATTCTGGA GGTAAATGGC 
ATGGATTTTA ACCACTTTAC CAGAGAAGAA GCAGCAATGT ATCTTATGAA CATACGTGGT 
GGAGAGCGTA TCGATATGCG GACTCAGAAC AAAGCAGACA TCTACAAGAA GATTCTGAAG 
TCCAACCTTG GAGATTCTTT CTACATCCGC ACACACTATG ATCATGTGGC AGACAGCAGC 
GTCGGCTTAA GCTTTACCAG AGGCGAGGTG TTCAGAGTGG TGGACACTAT GCATCGAGGA 
AAGCTGGGGA CCTGGTTAGC TGTGCGCATG GGCAATGATC TACACGAACT GGACAAAGGG 
ACCATTCCTA ACCAGACCAG GGCTGAAACT CTGGCTAATT TGGAGCAGGC GCAGAGAATT 
AGTGCAGCTG AACGTCAGGC ATCTGGCCCC AGAGCTGAAT TCTGGAAGCT ACGGGGTCTC 
AGAGGGGCCA AAAAGAGTAA CCGAAGAACC CGTGATGACC TCCTGCAGTT GACAATTCAA 
GGCAAATTTC CAGCATACGA GAAGGTCCTG TTGAAAGAAG CCAATTTCAA ACGTCCTATT 
GTCATCTTGG GTCCTCTGAA TGACATTGCC AATGAAAAAC TGGCCCGAGA GTTGCCTGAT 
GAATTTGAAA TTGCAGAAAT GGTACCCAGG AGTGGAAATG ATAGTTCATC AAGTGTAATC 
AAACTGGACA CCGTAAGGCG GATAGCGGAG CAGGACAAGC ATCCCTTGCT GGACATTACA 
CCCACTGCCA TAGAAAGATT GAATTATATC CAGTACCATC CCATGGTGCT CTTCTTGGAT 
CCTCATAGTC GGAAGGACGT GAAGTCTATG AGGCAAAAAT ACATGCCAGG CTCCAGCAAG 
AGCTCTCGAC GCCTCTATGC CCAGGCCTTA AAGCTAAGGA AAAACTACAG CACTCTGTTT 
TCTGCACGCA TTGATCTTCA GCCAAATACA AACATGTGGT ATGATGTTTT GAAAGACAAG 
ATTCGGCAAC AGCAGTCTAA GCCTGTCTGG GTTTCTGAAG TAGCGATGGA AGGAGGAGCC 
GAAGAGGATC TTGACGCATT TAATCGAGCA AACAACTCTG ACTATCTCAG CGGTGCCAGT 
GACTACGAGG ACACTGACGG TGAGGCCTTC ACAGACGGTG AAGCCTACAC GGACAATGAA 
GACCTGGAGG AACCCTTAGG AGGCATTGCC AAACACCGCA GCTCTGCGCT GGCCCGTTCT 
TCGGAGCCTG CCAGTGTGTT AAATCATAGC CCTGAACCTC CCTACAACCT ACACGAGGAG 
GAGGAAGAGC CAGAGTACAC CCTACCGCCC AGTGAAGTTC CTCCCATCCT CCATGTTCCC 
GAGCCCCGAT CTCCACGCCT TGTTGCTGGA ATACCAGAAG AGCAAGAGGA TGTGCCCTCG 
CAGCGCAGTT TCACAGATTC AGACTTCAGC AATGATGATG TTGTTGCACC TCAAACACCT 
TCCGATGCGC CACCCAAATT CAGGGCACCA GATCCCAAAG ATGTCCAGTC AAAGACCCCT 
CCACTGTCAG CCATAGAGGA GAGACTACAA CAGGCTCGCA TGGCAGAACA GATTGAACCA 
GAAAGGAAAC CTTCGCCTTC TTTTATAGTA CTCGCACATC ATGAAGCCAT GCAAATGCGG 
AGAACGCAAA TCCGAGGAAG CGACAGTGAC AGCGACGAAA ACGACAGAGA TGAAGACGAA 
TATGACGAAG CAGAAGACAT TGAATGGGGA CCTGCTACAG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170499.1
CDS	188..3955
Translation	MEEMTIWEQHTVTLSKDSKVGFGFAVSGGLDKPNPANGDAAVVVSDVIPTGPAMGRLQTRDQIVMVNGVSMENVTSLYTIQNLRKCGKTANITVKRPRTIQIPASSRPTRSASQSNILDDKGPQYKGQRGPDTTRTRRARSVTPDRNGHDLPITSGYKRLPNQDVQNKPIKTTLIKKKPTDEYGMKLGSQIFIKHMTPTGLAAKEGSLQEGDLVLKINGMTTENLSLLETKHLVEKSRGNLTMLVLRDDRKFLVSIPEVEDSPQNSSDERREDSSSELEEISDLDSDLPVTNRASRRKDRKSNRNRADPVSKKLESPPLRSTLTRPAQSMPPRRVPSESESDRSNSPPPRHDTLDHRNKVLSGYSTLPNPRASPITSSSTSRPRRAVSESDSDRSASPPPRRESPRPDNHSRYKVLPDLPPPGLKPSSLSMSQDFPRRMASPYRPPPRDASESESDESARPQRRQGTPASQESRNRYRAAPEVKAAAVMEPPKWAKPSTPVQRAPSESESDGSYNSPPKQPPSPVSKSSRIKGPEMQMPITSIRQDPPLRAAPARPPPEDSSDSERAQSPLRRSDSHNSDHSFPRANGTLRSNVSIPNHTALTSKAVEEPLYVLPPDAVPTPNLGYSSDQNHISFVKEGSVGLRLVGGNDVGIFVGGVQPNSPAQKQGITEGDQILEVNGMDFNHFTREEAAMYLMNIRGGERIDMRTQNKADIYKKILKSNLGDSFYIRTHYDHVADSSVGLSFTRGEVFRVVDTMHRGKLGTWLAVRMGNDLHELDKGTIPNQTRAETLANLEQAQRISAAERQASGPRAEFWKLRGLRGAKKSNRRTRDDLLQLTIQGKFPAYEKVLLKEANFKRPIVILGPLNDIANEKLARELPDEFEIAEMVPRSGNDSSSSVIKLDTVRRIAEQDKHPLLDITPTAIERLNYIQYHPMVLFLDPHSRKDVKSMRQKYMPGSSKSSRRLYAQALKLRKNYSTLFSARIDLQPNTNMWYDVLKDKIRQQQSKPVWVSEVAMEGGAEEDLDAFNRANNSDYLSGASDYEDTDGEAFTDGEAYTDNEDLEEPLGGIAKHRSSALARSSEPASVLNHSPEPPYNLHEEEEEPEYTLPPSEVPPILHVPEPRSPRLVAGIPEEQEDVPSQRSFTDSDFSNDDVVAPQTPSDAPPKFRAPDPKDVQSKTPPLSAIEERLQQARMAEQIEPERKPSPSFIVLAHHEAMQMRRTQIRGSDSDSDENDRDEDEYDEAEDIEWGPATEL

Target ORF information:

RefSeq Version	XM_005170442.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170442.3

ORF Insert Sequence:

ATGGAGGAGA TGACGATATG GGAGCAGCAC ACGGTCACGT TATCGAAAGA TTCCAAGGTG 
GGCTTTGGTT TTGCCGTCTC AGGTGGGCTG GACAAACCCA ACCCAGCAAA TGGGGACGCG 
GCAGTGGTGG TCTCTGATGT GATTCCCACC GGTCCCGCCA TGGGCCGACT TCAGACGCGA 
GATCAGATTG TTATGGTGAA TGGTGTTTCA ATGGAGAACG TCACTTCACT CTACACCATA 
CAGAATCTGA GGAAGTGCGG GAAGACCGCT AACATTACTG TGAAGAGGCC GCGCACGATC 
CAGATTCCTG CCAGCAGCAG ACCCACTCGC TCAGCCTCTC AGTCCAACAT TTTAGACGAT 
AAAGGACCTC AGTATAAGGG TCAGCGGGGC CCTGACACCA CACGCACCCG CCGGGCCCGA 
AGCGTCACTC CTGACCGCAA TGGCCACGAT CTGCCCATCA CATCCGGATA CAAGCGACTG 
CCCAATCAGG ACGTCCAGAA CAAACCCATC AAGACCACTC TGATCAAGAA GAAGCCCACC 
GACGAGTATG GAATGAAGCT GGGGAGCCAG ATCTTCATCA AGCACATGAC TCCCACCGGT 
CTGGCTGCCA AAGAAGGCTC GCTTCAGGAG GGAGATCTGG TTTTAAAGAT TAACGGCATG 
ACAACAGAAA ACCTGTCCCT GCTGGAGACC AAGCATCTGG TGGAGAAATC CAGAGGGAAC 
CTGACCATGC TGGTGCTGAG AGACGACAGA AAGTTTCTGG TCAGCATCCC TGAAGTGGAG 
GACAGTCCTC AAAACAGCTC AGACGAGAGG CGGGAGGACA GCAGCTCTGA GCTGGAGGAA 
ATCTCAGACC TGGATTCGGA CCTTCCCGTT ACAAACCGAG CATCTCGTCG CAAAGACCGA 
AAATCGAACA GAAACCGGGC TGATCCTGTG TCGAAAAAAC TTGAATCCCC TCCTCTGCGC 
TCAACTCTCA CAAGACCTGC TCAGTCTATG CCTCCTCGCA GAGTTCCCTC GGAGTCTGAA 
TCTGATCGCA GCAACTCTCC TCCACCAAGA CATGACACTC TCGATCACAG AAACAAGGTT 
CTCTCTGGGT ACTCCACACT GCCAAACCCT CGAGCTTCAC CCATCACCTC CTCCTCCACA 
TCCCGGCCTC GCAGAGCCGT TTCTGAGTCA GATTCAGACC GGAGCGCATC TCCACCGCCC 
AGACGTGAGA GTCCCAGACC TGATAACCAC TCCAGATACA AGGTGCTTCC TGATCTCCCC 
CCTCCAGGTT TGAAGCCTTC TTCTCTTAGC ATGTCTCAGG ATTTCCCTCG CCGCATGGCT 
TCACCTTACA GGCCTCCACC TAGAGATGCT TCAGAGTCTG AATCGGACGA AAGTGCCAGA 
CCTCAGCGCA GACAGGGGAC TCCGGCCAGC CAAGAGTCCC GCAATAGATA CAGGGCTGCC 
CCTGAAGTGA AGGCAGCAGC AGTAATGGAG CCCCCAAAAT GGGCGAAACC CTCAACGCCT 
GTACAGAGAG CTCCCTCAGA ATCTGAATCA GATGGAAGTT ACAACTCTCC ACCCAAACAA 
CCTCCGTCTC CAGTCAGTAA AAGCTCCAGA ATCAAAGGGC CTGAAATGCA AATGCCAATC 
ACCAGCATCC GGCAGGATCC TCCACTCAGA GCCGCTCCTG CCAGACCACC TCCCGAAGAT 
TCCTCTGACT CAGAAAGAGC CCAGTCACCT CTCAGGAGAT CTGACAGTCA CAACAGCGAT 
CACAGTTTCC CACGTGCTAA TGGGACTCTC CGCTCGAACG TCTCCATCCC CAACCACACT 
GCGCTGACTT CTAAAGCTGT AGAAGAACCA CTTTATGTGC TTCCACCAGA CGCAGTGCCA 
ACACCAAACC TGGGGTACAG CTCCGATCAG AACCATATCA GCTTTGTGAA GGAAGGCAGT 
GTCGGCTTGA GACTTGTGGG AGGAAATGAT GTTGGCATCT TTGTCGGTGG TGTCCAGCCT 
AACAGTCCAG CCCAAAAGCA AGGCATAACA GAGGGTGATC AGATTCTGGA GGTAAATGGC 
ATGGATTTTA ACCACTTTAC CAGAGAAGAA GCAGCAATGT ATCTTATGAA CATACGTGGT 
GGAGAGCGTA TCGATATGCG GACTCAGAAC AAAGCAGACA TCTACAAGAA GATTCTGAAG 
TCCAACCTTG GAGATTCTTT CTACATCCGC ACACACTATG ATCATGTGGC AGACAGCAGC 
GTCGGCTTAA GCTTTACCAG AGGCGAGGTG TTCAGAGTGG TGGACACTAT GCATCGAGGA 
AAGCTGGGGA CCTGGTTAGC TGTGCGCATG GGCAATGATC TACACGAACT GGACAAAGGG 
ACCATTCCTA ACCAGACCAG GGCTGAAACT CTGGCTAATT TGGAGCAGGC GCAGAGAATT 
AGTGCAGCTG AACGTCAGGC ATCTGGCCCC AGAGCTGAAT TCTGGAAGCT ACGGGGTCTC 
AGAGGGGCCA AAAAGAGTAA CCGAAGAACC CGTGATGACC TCCTGCAGTT GACAATTCAA 
GGCAAATTTC CAGCATACGA GAAGGTCCTG TTGAAAGAAG CCAATTTCAA ACGTCCTATT 
GTCATCTTGG GTCCTCTGAA TGACATTGCC AATGAAAAAC TGGCCCGAGA GTTGCCTGAT 
GAATTTGAAA TTGCAGAAAT GGTACCCAGG AGTGGAAATG ATAGTTCATC AAGTGTAATC 
AAACTGGACA CCGTAAGGCG GATAGCGGAG CAGGACAAGC ATCCCTTGCT GGACATTACA 
CCCACTGCCA TAGAAAGATT GAATTATATC CAGTACCATC CCATGGTGCT CTTCTTGGAT 
CCTCATAGTC GGAAGGACGT GAAGTCTATG AGGCAAAAAT ACATGCCAGG CTCCAGCAAG 
AGCTCTCGAC GCCTCTATGC CCAGGCCTTA AAGCTAAGGA AAAACTACAG CACTCTGTTT 
TCTGCACGCA TTGATCTTCA GCCAAATACA AACATGTGGT ATGATGTTTT GAAAGACAAG 
ATTCGGCAAC AGCAGTCTAA GCCTGTCTGG GTTTCTGAAG TAGCGATGGA AGGAGGAGCC 
GAAGAGGATC TTGACGCATT TAATCGAGCA AACAACTCTG ACTATCTCAG CGGTGCCAGT 
GACTACGAGG ACACTGACGG TGAGGCCTTC ACAGACGGTG AAGCCTACAC GGACAATGAA 
GACCTGGAGG AACCCTTAGG AGGCATTGCC AAACACCGCA GCTCTGCGCT GGCCCGTTCT 
TCGGAGCCTG CCAGTGTGTT AAATCATAGC CCTGAACCTC CCTACAACCT ACACGAGGAG 
GAGGAAGAGC CAGAGTACAC CCTACCGCCC AGTGAAGTTC CTCCCATCCT CCATGTTCCC 
GAGCCCCGAT CTCCACGCCT TGTTGCTGGA ATACCAGAAG AGCAAGAGGA TGTGCCCTCG 
CAGCGCAGTT TCACAGATTC AGACTTCAGC AATGATGATG TTGTTGCACC TCAAACACCT 
TCCGATGCGC CACCCAAATT CAGGGCACCA GATCCCAAAG ATGTCCAGTC AAAGACCCCT 
CCACTGTCAG CCATAGAGGA GAGACTACAA CAGGCTCGCA TGGCAGAACA GATTGAACCA 
GAAAGGAAAC CTTCGCCTTC TTTTATAGTA CTCGCACATC ATGAAGCCAT GCAAATGCGG 
AGAACGCAAA TCCGAGGAAG CGACAGTGAC AGCGACGAAA ACGACAGAGA TGAAGACGAA 
TATGACGAAG CAGAAGACAT TGAATGGGGA CCTGCTACAG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_005170442.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170499.1 Danio rerio tight junction protein 3 (tjp3), transcript variant X1, mRNA.

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CloneID	ODa26853
Clone ID Related Accession (Same CDS sequence)	NM_001328340.1 , XM_005170442.3 , XM_005170442.4
Accession Version	XM_005170442.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction protein ZO-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170442.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA TGACGATATG GGAGCAGCAC ACGGTCACGT TATCGAAAGA TTCCAAGGTG 
GGCTTTGGTT TTGCCGTCTC AGGTGGGCTG GACAAACCCA ACCCAGCAAA TGGGGACGCG 
GCAGTGGTGG TCTCTGATGT GATTCCCACC GGTCCCGCCA TGGGCCGACT TCAGACGCGA 
GATCAGATTG TTATGGTGAA TGGTGTTTCA ATGGAGAACG TCACTTCACT CTACACCATA 
CAGAATCTGA GGAAGTGCGG GAAGACCGCT AACATTACTG TGAAGAGGCC GCGCACGATC 
CAGATTCCTG CCAGCAGCAG ACCCACTCGC TCAGCCTCTC AGTCCAACAT TTTAGACGAT 
AAAGGACCTC AGTATAAGGG TCAGCGGGGC CCTGACACCA CACGCACCCG CCGGGCCCGA 
AGCGTCACTC CTGACCGCAA TGGCCACGAT CTGCCCATCA CATCCGGATA CAAGCGACTG 
CCCAATCAGG ACGTCCAGAA CAAACCCATC AAGACCACTC TGATCAAGAA GAAGCCCACC 
GACGAGTATG GAATGAAGCT GGGGAGCCAG ATCTTCATCA AGCACATGAC TCCCACCGGT 
CTGGCTGCCA AAGAAGGCTC GCTTCAGGAG GGAGATCTGG TTTTAAAGAT TAACGGCATG 
ACAACAGAAA ACCTGTCCCT GCTGGAGACC AAGCATCTGG TGGAGAAATC CAGAGGGAAC 
CTGACCATGC TGGTGCTGAG AGACGACAGA AAGTTTCTGG TCAGCATCCC TGAAGTGGAG 
GACAGTCCTC AAAACAGCTC AGACGAGAGG CGGGAGGACA GCAGCTCTGA GCTGGAGGAA 
ATCTCAGACC TGGATTCGGA CCTTCCCGTT ACAAACCGAG CATCTCGTCG CAAAGACCGA 
AAATCGAACA GAAACCGGGC TGATCCTGTG TCGAAAAAAC TTGAATCCCC TCCTCTGCGC 
TCAACTCTCA CAAGACCTGC TCAGTCTATG CCTCCTCGCA GAGTTCCCTC GGAGTCTGAA 
TCTGATCGCA GCAACTCTCC TCCACCAAGA CATGACACTC TCGATCACAG AAACAAGGTT 
CTCTCTGGGT ACTCCACACT GCCAAACCCT CGAGCTTCAC CCATCACCTC CTCCTCCACA 
TCCCGGCCTC GCAGAGCCGT TTCTGAGTCA GATTCAGACC GGAGCGCATC TCCACCGCCC 
AGACGTGAGA GTCCCAGACC TGATAACCAC TCCAGATACA AGGTGCTTCC TGATCTCCCC 
CCTCCAGGTT TGAAGCCTTC TTCTCTTAGC ATGTCTCAGG ATTTCCCTCG CCGCATGGCT 
TCACCTTACA GGCCTCCACC TAGAGATGCT TCAGAGTCTG AATCGGACGA AAGTGCCAGA 
CCTCAGCGCA GACAGGGGAC TCCGGCCAGC CAAGAGTCCC GCAATAGATA CAGGGCTGCC 
CCTGAAGTGA AGGCAGCAGC AGTAATGGAG CCCCCAAAAT GGGCGAAACC CTCAACGCCT 
GTACAGAGAG CTCCCTCAGA ATCTGAATCA GATGGAAGTT ACAACTCTCC ACCCAAACAA 
CCTCCGTCTC CAGTCAGTAA AAGCTCCAGA ATCAAAGGGC CTGAAATGCA AATGCCAATC 
ACCAGCATCC GGCAGGATCC TCCACTCAGA GCCGCTCCTG CCAGACCACC TCCCGAAGAT 
TCCTCTGACT CAGAAAGAGC CCAGTCACCT CTCAGGAGAT CTGACAGTCA CAACAGCGAT 
CACAGTTTCC CACGTGCTAA TGGGACTCTC CGCTCGAACG TCTCCATCCC CAACCACACT 
GCGCTGACTT CTAAAGCTGT AGAAGAACCA CTTTATGTGC TTCCACCAGA CGCAGTGCCA 
ACACCAAACC TGGGGTACAG CTCCGATCAG AACCATATCA GCTTTGTGAA GGAAGGCAGT 
GTCGGCTTGA GACTTGTGGG AGGAAATGAT GTTGGCATCT TTGTCGGTGG TGTCCAGCCT 
AACAGTCCAG CCCAAAAGCA AGGCATAACA GAGGGTGATC AGATTCTGGA GGTAAATGGC 
ATGGATTTTA ACCACTTTAC CAGAGAAGAA GCAGCAATGT ATCTTATGAA CATACGTGGT 
GGAGAGCGTA TCGATATGCG GACTCAGAAC AAAGCAGACA TCTACAAGAA GATTCTGAAG 
TCCAACCTTG GAGATTCTTT CTACATCCGC ACACACTATG ATCATGTGGC AGACAGCAGC 
GTCGGCTTAA GCTTTACCAG AGGCGAGGTG TTCAGAGTGG TGGACACTAT GCATCGAGGA 
AAGCTGGGGA CCTGGTTAGC TGTGCGCATG GGCAATGATC TACACGAACT GGACAAAGGG 
ACCATTCCTA ACCAGACCAG GGCTGAAACT CTGGCTAATT TGGAGCAGGC GCAGAGAATT 
AGTGCAGCTG AACGTCAGGC ATCTGGCCCC AGAGCTGAAT TCTGGAAGCT ACGGGGTCTC 
AGAGGGGCCA AAAAGAGTAA CCGAAGAACC CGTGATGACC TCCTGCAGTT GACAATTCAA 
GGCAAATTTC CAGCATACGA GAAGGTCCTG TTGAAAGAAG CCAATTTCAA ACGTCCTATT 
GTCATCTTGG GTCCTCTGAA TGACATTGCC AATGAAAAAC TGGCCCGAGA GTTGCCTGAT 
GAATTTGAAA TTGCAGAAAT GGTACCCAGG AGTGGAAATG ATAGTTCATC AAGTGTAATC 
AAACTGGACA CCGTAAGGCG GATAGCGGAG CAGGACAAGC ATCCCTTGCT GGACATTACA 
CCCACTGCCA TAGAAAGATT GAATTATATC CAGTACCATC CCATGGTGCT CTTCTTGGAT 
CCTCATAGTC GGAAGGACGT GAAGTCTATG AGGCAAAAAT ACATGCCAGG CTCCAGCAAG 
AGCTCTCGAC GCCTCTATGC CCAGGCCTTA AAGCTAAGGA AAAACTACAG CACTCTGTTT 
TCTGCACGCA TTGATCTTCA GCCAAATACA AACATGTGGT ATGATGTTTT GAAAGACAAG 
ATTCGGCAAC AGCAGTCTAA GCCTGTCTGG GTTTCTGAAG TAGCGATGGA AGGAGGAGCC 
GAAGAGGATC TTGACGCATT TAATCGAGCA AACAACTCTG ACTATCTCAG CGGTGCCAGT 
GACTACGAGG ACACTGACGG TGAGGCCTTC ACAGACGGTG AAGCCTACAC GGACAATGAA 
GACCTGGAGG AACCCTTAGG AGGCATTGCC AAACACCGCA GCTCTGCGCT GGCCCGTTCT 
TCGGAGCCTG CCAGTGTGTT AAATCATAGC CCTGAACCTC CCTACAACCT ACACGAGGAG 
GAGGAAGAGC CAGAGTACAC CCTACCGCCC AGTGAAGTTC CTCCCATCCT CCATGTTCCC 
GAGCCCCGAT CTCCACGCCT TGTTGCTGGA ATACCAGAAG AGCAAGAGGA TGTGCCCTCG 
CAGCGCAGTT TCACAGATTC AGACTTCAGC AATGATGATG TTGTTGCACC TCAAACACCT 
TCCGATGCGC CACCCAAATT CAGGGCACCA GATCCCAAAG ATGTCCAGTC AAAGACCCCT 
CCACTGTCAG CCATAGAGGA GAGACTACAA CAGGCTCGCA TGGCAGAACA GATTGAACCA 
GAAAGGAAAC CTTCGCCTTC TTTTATAGTA CTCGCACATC ATGAAGCCAT GCAAATGCGG 
AGAACGCAAA TCCGAGGAAG CGACAGTGAC AGCGACGAAA ACGACAGAGA TGAAGACGAA 
TATGACGAAG CAGAAGACAT TGAATGGGGA CCTGCTACAG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170499.1
CDS	384..4151
Translation	MEEMTIWEQHTVTLSKDSKVGFGFAVSGGLDKPNPANGDAAVVVSDVIPTGPAMGRLQTRDQIVMVNGVSMENVTSLYTIQNLRKCGKTANITVKRPRTIQIPASSRPTRSASQSNILDDKGPQYKGQRGPDTTRTRRARSVTPDRNGHDLPITSGYKRLPNQDVQNKPIKTTLIKKKPTDEYGMKLGSQIFIKHMTPTGLAAKEGSLQEGDLVLKINGMTTENLSLLETKHLVEKSRGNLTMLVLRDDRKFLVSIPEVEDSPQNSSDERREDSSSELEEISDLDSDLPVTNRASRRKDRKSNRNRADPVSKKLESPPLRSTLTRPAQSMPPRRVPSESESDRSNSPPPRHDTLDHRNKVLSGYSTLPNPRASPITSSSTSRPRRAVSESDSDRSASPPPRRESPRPDNHSRYKVLPDLPPPGLKPSSLSMSQDFPRRMASPYRPPPRDASESESDESARPQRRQGTPASQESRNRYRAAPEVKAAAVMEPPKWAKPSTPVQRAPSESESDGSYNSPPKQPPSPVSKSSRIKGPEMQMPITSIRQDPPLRAAPARPPPEDSSDSERAQSPLRRSDSHNSDHSFPRANGTLRSNVSIPNHTALTSKAVEEPLYVLPPDAVPTPNLGYSSDQNHISFVKEGSVGLRLVGGNDVGIFVGGVQPNSPAQKQGITEGDQILEVNGMDFNHFTREEAAMYLMNIRGGERIDMRTQNKADIYKKILKSNLGDSFYIRTHYDHVADSSVGLSFTRGEVFRVVDTMHRGKLGTWLAVRMGNDLHELDKGTIPNQTRAETLANLEQAQRISAAERQASGPRAEFWKLRGLRGAKKSNRRTRDDLLQLTIQGKFPAYEKVLLKEANFKRPIVILGPLNDIANEKLARELPDEFEIAEMVPRSGNDSSSSVIKLDTVRRIAEQDKHPLLDITPTAIERLNYIQYHPMVLFLDPHSRKDVKSMRQKYMPGSSKSSRRLYAQALKLRKNYSTLFSARIDLQPNTNMWYDVLKDKIRQQQSKPVWVSEVAMEGGAEEDLDAFNRANNSDYLSGASDYEDTDGEAFTDGEAYTDNEDLEEPLGGIAKHRSSALARSSEPASVLNHSPEPPYNLHEEEEEPEYTLPPSEVPPILHVPEPRSPRLVAGIPEEQEDVPSQRSFTDSDFSNDDVVAPQTPSDAPPKFRAPDPKDVQSKTPPLSAIEERLQQARMAEQIEPERKPSPSFIVLAHHEAMQMRRTQIRGSDSDSDENDRDEDEYDEAEDIEWGPATEL

Target ORF information:

RefSeq Version	XM_005170442.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170442.4

ORF Insert Sequence:

ATGGAGGAGA TGACGATATG GGAGCAGCAC ACGGTCACGT TATCGAAAGA TTCCAAGGTG 
GGCTTTGGTT TTGCCGTCTC AGGTGGGCTG GACAAACCCA ACCCAGCAAA TGGGGACGCG 
GCAGTGGTGG TCTCTGATGT GATTCCCACC GGTCCCGCCA TGGGCCGACT TCAGACGCGA 
GATCAGATTG TTATGGTGAA TGGTGTTTCA ATGGAGAACG TCACTTCACT CTACACCATA 
CAGAATCTGA GGAAGTGCGG GAAGACCGCT AACATTACTG TGAAGAGGCC GCGCACGATC 
CAGATTCCTG CCAGCAGCAG ACCCACTCGC TCAGCCTCTC AGTCCAACAT TTTAGACGAT 
AAAGGACCTC AGTATAAGGG TCAGCGGGGC CCTGACACCA CACGCACCCG CCGGGCCCGA 
AGCGTCACTC CTGACCGCAA TGGCCACGAT CTGCCCATCA CATCCGGATA CAAGCGACTG 
CCCAATCAGG ACGTCCAGAA CAAACCCATC AAGACCACTC TGATCAAGAA GAAGCCCACC 
GACGAGTATG GAATGAAGCT GGGGAGCCAG ATCTTCATCA AGCACATGAC TCCCACCGGT 
CTGGCTGCCA AAGAAGGCTC GCTTCAGGAG GGAGATCTGG TTTTAAAGAT TAACGGCATG 
ACAACAGAAA ACCTGTCCCT GCTGGAGACC AAGCATCTGG TGGAGAAATC CAGAGGGAAC 
CTGACCATGC TGGTGCTGAG AGACGACAGA AAGTTTCTGG TCAGCATCCC TGAAGTGGAG 
GACAGTCCTC AAAACAGCTC AGACGAGAGG CGGGAGGACA GCAGCTCTGA GCTGGAGGAA 
ATCTCAGACC TGGATTCGGA CCTTCCCGTT ACAAACCGAG CATCTCGTCG CAAAGACCGA 
AAATCGAACA GAAACCGGGC TGATCCTGTG TCGAAAAAAC TTGAATCCCC TCCTCTGCGC 
TCAACTCTCA CAAGACCTGC TCAGTCTATG CCTCCTCGCA GAGTTCCCTC GGAGTCTGAA 
TCTGATCGCA GCAACTCTCC TCCACCAAGA CATGACACTC TCGATCACAG AAACAAGGTT 
CTCTCTGGGT ACTCCACACT GCCAAACCCT CGAGCTTCAC CCATCACCTC CTCCTCCACA 
TCCCGGCCTC GCAGAGCCGT TTCTGAGTCA GATTCAGACC GGAGCGCATC TCCACCGCCC 
AGACGTGAGA GTCCCAGACC TGATAACCAC TCCAGATACA AGGTGCTTCC TGATCTCCCC 
CCTCCAGGTT TGAAGCCTTC TTCTCTTAGC ATGTCTCAGG ATTTCCCTCG CCGCATGGCT 
TCACCTTACA GGCCTCCACC TAGAGATGCT TCAGAGTCTG AATCGGACGA AAGTGCCAGA 
CCTCAGCGCA GACAGGGGAC TCCGGCCAGC CAAGAGTCCC GCAATAGATA CAGGGCTGCC 
CCTGAAGTGA AGGCAGCAGC AGTAATGGAG CCCCCAAAAT GGGCGAAACC CTCAACGCCT 
GTACAGAGAG CTCCCTCAGA ATCTGAATCA GATGGAAGTT ACAACTCTCC ACCCAAACAA 
CCTCCGTCTC CAGTCAGTAA AAGCTCCAGA ATCAAAGGGC CTGAAATGCA AATGCCAATC 
ACCAGCATCC GGCAGGATCC TCCACTCAGA GCCGCTCCTG CCAGACCACC TCCCGAAGAT 
TCCTCTGACT CAGAAAGAGC CCAGTCACCT CTCAGGAGAT CTGACAGTCA CAACAGCGAT 
CACAGTTTCC CACGTGCTAA TGGGACTCTC CGCTCGAACG TCTCCATCCC CAACCACACT 
GCGCTGACTT CTAAAGCTGT AGAAGAACCA CTTTATGTGC TTCCACCAGA CGCAGTGCCA 
ACACCAAACC TGGGGTACAG CTCCGATCAG AACCATATCA GCTTTGTGAA GGAAGGCAGT 
GTCGGCTTGA GACTTGTGGG AGGAAATGAT GTTGGCATCT TTGTCGGTGG TGTCCAGCCT 
AACAGTCCAG CCCAAAAGCA AGGCATAACA GAGGGTGATC AGATTCTGGA GGTAAATGGC 
ATGGATTTTA ACCACTTTAC CAGAGAAGAA GCAGCAATGT ATCTTATGAA CATACGTGGT 
GGAGAGCGTA TCGATATGCG GACTCAGAAC AAAGCAGACA TCTACAAGAA GATTCTGAAG 
TCCAACCTTG GAGATTCTTT CTACATCCGC ACACACTATG ATCATGTGGC AGACAGCAGC 
GTCGGCTTAA GCTTTACCAG AGGCGAGGTG TTCAGAGTGG TGGACACTAT GCATCGAGGA 
AAGCTGGGGA CCTGGTTAGC TGTGCGCATG GGCAATGATC TACACGAACT GGACAAAGGG 
ACCATTCCTA ACCAGACCAG GGCTGAAACT CTGGCTAATT TGGAGCAGGC GCAGAGAATT 
AGTGCAGCTG AACGTCAGGC ATCTGGCCCC AGAGCTGAAT TCTGGAAGCT ACGGGGTCTC 
AGAGGGGCCA AAAAGAGTAA CCGAAGAACC CGTGATGACC TCCTGCAGTT GACAATTCAA 
GGCAAATTTC CAGCATACGA GAAGGTCCTG TTGAAAGAAG CCAATTTCAA ACGTCCTATT 
GTCATCTTGG GTCCTCTGAA TGACATTGCC AATGAAAAAC TGGCCCGAGA GTTGCCTGAT 
GAATTTGAAA TTGCAGAAAT GGTACCCAGG AGTGGAAATG ATAGTTCATC AAGTGTAATC 
AAACTGGACA CCGTAAGGCG GATAGCGGAG CAGGACAAGC ATCCCTTGCT GGACATTACA 
CCCACTGCCA TAGAAAGATT GAATTATATC CAGTACCATC CCATGGTGCT CTTCTTGGAT 
CCTCATAGTC GGAAGGACGT GAAGTCTATG AGGCAAAAAT ACATGCCAGG CTCCAGCAAG 
AGCTCTCGAC GCCTCTATGC CCAGGCCTTA AAGCTAAGGA AAAACTACAG CACTCTGTTT 
TCTGCACGCA TTGATCTTCA GCCAAATACA AACATGTGGT ATGATGTTTT GAAAGACAAG 
ATTCGGCAAC AGCAGTCTAA GCCTGTCTGG GTTTCTGAAG TAGCGATGGA AGGAGGAGCC 
GAAGAGGATC TTGACGCATT TAATCGAGCA AACAACTCTG ACTATCTCAG CGGTGCCAGT 
GACTACGAGG ACACTGACGG TGAGGCCTTC ACAGACGGTG AAGCCTACAC GGACAATGAA 
GACCTGGAGG AACCCTTAGG AGGCATTGCC AAACACCGCA GCTCTGCGCT GGCCCGTTCT 
TCGGAGCCTG CCAGTGTGTT AAATCATAGC CCTGAACCTC CCTACAACCT ACACGAGGAG 
GAGGAAGAGC CAGAGTACAC CCTACCGCCC AGTGAAGTTC CTCCCATCCT CCATGTTCCC 
GAGCCCCGAT CTCCACGCCT TGTTGCTGGA ATACCAGAAG AGCAAGAGGA TGTGCCCTCG 
CAGCGCAGTT TCACAGATTC AGACTTCAGC AATGATGATG TTGTTGCACC TCAAACACCT 
TCCGATGCGC CACCCAAATT CAGGGCACCA GATCCCAAAG ATGTCCAGTC AAAGACCCCT 
CCACTGTCAG CCATAGAGGA GAGACTACAA CAGGCTCGCA TGGCAGAACA GATTGAACCA 
GAAAGGAAAC CTTCGCCTTC TTTTATAGTA CTCGCACATC ATGAAGCCAT GCAAATGCGG 
AGAACGCAAA TCCGAGGAAG CGACAGTGAC AGCGACGAAA ACGACAGAGA TGAAGACGAA 
TATGACGAAG CAGAAGACAT TGAATGGGGA CCTGCTACAG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Rapid reverse genetic screening using CRISPR in zebrafish.
Nature methods12(6)535-40(2015 Jun)
Shah AN,Davey CF,Whitebirch AC,Miller AC,Moens CB

Temporal and spatial expression of tight junction genes during zebrafish pronephros development.
Gene expression patterns : GEP16(2)104-13(2014 Nov)
McKee R,Gerlach GF,Jou J,Cheng CN,Wingert RA

Unilateral dampening of Bmp activity by nodal generates cardiac left-right asymmetry.
Developmental cell24(6)660-7(2013 Mar)
Veerkamp J,Rudolph F,Cseresnyes Z,Priller F,Otten C,Renz M,Schaefer L,Abdelilah-Seyfried S

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tjp3 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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