YTHDF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	YTHDF1
Entrez Gene ID	327606
Full Name	YTH N(6)-methyladenosine RNA binding protein 1
Synonyms	fb49c02,wu:fb49c02,wu:fi19h06,wu:fj87f09,wu:fp06c11,zgc:55657
General protein information	Preferred Names YTH N(6)-methyladenosine RNA binding protein 1 Names YTH domain-containing family protein 1 YTH domain family, member 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212713.1	NP_997878.1	YTH domain-containing family protein 1
XM_005167004.4	XP_005167061.1	YTH domain-containing family protein 1 isoform X1
XM_005167004.3	XP_005167061.1	YTH domain-containing family protein 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ythdf1	NP_001019927.1
Gallus gallus (chicken)	YTHDF1	NP_001012851.1
Danio rerio (zebrafish)	ythdf1	NP_997878.1
Xenopus tropicalis (tropical clawed frog)	ythdf1	NP_989392.1
	YTHDF1	XP_002798106.1
Mus musculus (house mouse)	Ythdf1	NP_776122.1
Pan troglodytes (chimpanzee)	YTHDF1	XP_003317109.1
Canis lupus familiaris (dog)	YTHDF1	XP_005635335.1
Homo sapiens (human)	YTHDF1	NP_060268.2
Bos taurus (cattle)	YTHDF1	NP_001178345.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Despic V, Dejung M, Gu M, Krishnan J, Zhang J, Herzel L, Straube K, Gerstein MB, Butter F, Neugebauer KM
Genome research27(7)1184-1194(2017 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Concomitant duplications of opioid peptide and receptor genes before the origin of jawed vertebrates.
Sundstr?m G, Dreborg S, Larhammar D
PloS one5(5)e10512(2010 May)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YTHDF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YTHDF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32192	NM_212713.1 Latest version!	Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa14193	XM_005167004.4 Latest version!	Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa14193	XM_005167004.3	Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YTHDF1 ( NM_212713.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_997878.1 Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), mRNA.

Price & Availability↑

CloneID	ODa32192
Clone ID Related Accession (Same CDS sequence)	NM_212713.1
Accession Version	NM_212713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	YTH domain-containing family protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046885.1. ##Evidence-Data-START## Transcript exon combination :: BC046885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTACCA CCAGTATTGA CCCACAGACA TCAAAAGGGC AAGACGCTAA AGTGCAAAAT 
GGCTCTCTTC ATCAGAAGGA AACGGTCCAC GACAATGACT TTGAACCATA CCTCACTGGC 
CAGTCTAATC CGAATAACAG TTACCAATCC ATGACCGACC CATACCTGTC TAGCTACTAC 
GCCCCCTCAA TTGGGTTCCC GTACCCCCTC AGCGAGGCTC CCTGGTCCAC AGGTGGAGAC 
CCGCCGATCC CATACCTCAC TCCGTATGCC CCCCTCAGCA ATGGAGACCA TCACTTCATG 
CATGACACTG TGTTTGGACA GCCGGGGGGT CTGGGCAGTA GCATTTATCC ACACCGCTTT 
AACTTTTTCC CCGAAAACCC TGCGTTTTCT GCCTGGGGTA CGAGTGGCTC CCAGGGCCAG 
CAGACTCAGA GTTCTGCATA TGGGGGCAGC TACAGTTACC CGCCCAGTTC TTTAGGTGGA 
ACCCTGGTGC CAGACGGGCA AACAGGCTTC CCTAGTGACA CCTTGAATAA GGCACCAGGT 
ATGAATAGCT TGGAGCAGGG CATGGTGGGG CTGAAGATCG GTGGTGATGT GACTGGAGGT 
GGTTCCGGAG TTAAGACGGT GGGCTCCGTG ATTGGAGGAG GGCCGGTGGC TGCGGCGGTG 
GCCACGGGTA ATGGTGGGAC TCCTATTGGA CTGCCGCCCC CTAAGCCCAC ATCTTGGGCA 
GCCATTGCTA GCAAACCGGC CAAGCCTCAG CAACTGAAGG TGAAGAGTAA GCCAGGGATG 
CCCATGGCTG GAGGCACCCT GCCTCCGCCG CCCATCAAAC ACAACATGGA CATTGGCACT 
TGGGATAATA AGGGGCCTGT GACCAAAGTG GCATCATCAT TGCCACCTCA CCACCACCAC 
CAGCAGCAGC CTCCCCTCCA CTCCCAGGGT CACCTTCCAC CTCACCCTCA TGGACTGCCA 
CCCCCACCTC AGCCCCCAAT GCCGTCTGCA CAATCACTTG CCCAGCAGAT GGCCATGCAG 
GGCCCACCCC CTCCGCAGCC TTACCAGAAC CACGCCCCTG CTCCTCCCCC GCAAACTCGG 
TGGGTTGCCC CACGCAATCG CAACCCTGGT TATGGTGGTG GAGGAAGCGT GGACAGCAGT 
GGCTCCTCGA GTGGTGGAGG GGTCGGCAAT GGAGGCGGAG GTGGCCCACC GGGCTCCGGC 
GCCATCGAGT CCCATCCCGT CTTAGAAAAG CTGCGTGCGG CACACAGCTA CAACCCCAAG 
GAGTTCGATT GGAACCTGAA AAATGGCCGG GTGTTCATCA TTAAGAGCTA CTCTGAGGAC 
GACATCCATC GCTCCATCAA GTACTCCATC TGGTGCAGCA CGGAACATGG AAACAAGCGG 
CTGGATTCTG CGTTTCGTGC CATCAACGGC AAAGGTCCTG TCTACCTGCT GTTCAGCGTC 
AACGGCAGCG GACACTTCTG TGGCGTGGCG GAGATGCGTT CGCCTGTTGA CTACGGCACC 
AGTGCCGGTG TTTGGGCGCA GGACAAGTGG AAGGGCAAAT TTGATGTGGA CTGGCTGTTT 
GTTAAAGACG TGCCCAACAG TCAGCTCAGG CACATCCGCC TGGAGAACAA TGACAACAAG 
CCCGTGACCA ACTCGCGTGA CACACAGGAG GTGCCTCTGG AAAAGGCCAA GCAGGTGCTG 
AAGATCATCG CTACCTACAA ACACACAACC TCCATCTTCG ATGACTTCTC ACATTACGAG 
AAACGGCAGG AGGAGGAAGA GGTGGTAAGA AAGAATCTTG AGCCTGCTCC TATCCAGAAC 
CGATCCCGAC TGGATCAAGA ACGCCAGAAC CGAAATAAAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997878.1
CDS	332..2176
Translation	MSTTSIDPQTSKGQDAKVQNGSLHQKETVHDNDFEPYLTGQSNPNNSYQSMTDPYLSSYYAPSIGFPYPLSEAPWSTGGDPPIPYLTPYAPLSNGDHHFMHDTVFGQPGGLGSSIYPHRFNFFPENPAFSAWGTSGSQGQQTQSSAYGGSYSYPPSSLGGTLVPDGQTGFPSDTLNKAPGMNSLEQGMVGLKIGGDVTGGGSGVKTVGSVIGGGPVAAAVATGNGGTPIGLPPPKPTSWAAIASKPAKPQQLKVKSKPGMPMAGGTLPPPPIKHNMDIGTWDNKGPVTKVASSLPPHHHHQQQPPLHSQGHLPPHPHGLPPPPQPPMPSAQSLAQQMAMQGPPPPQPYQNHAPAPPPQTRWVAPRNRNPGYGGGGSVDSSGSSSGGGVGNGGGGGPPGSGAIESHPVLEKLRAAHSYNPKEFDWNLKNGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDSAFRAINGKGPVYLLFSVNGSGHFCGVAEMRSPVDYGTSAGVWAQDKWKGKFDVDWLFVKDVPNSQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATYKHTTSIFDDFSHYEKRQEEEEVVRKNLEPAPIQNRSRLDQERQNRNKQ

Target ORF information:

RefSeq Version	NM_212713.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212713.1

ORF Insert Sequence:

ATGTCTACCA CCAGTATTGA CCCACAGACA TCAAAAGGGC AAGACGCTAA AGTGCAAAAT 
GGCTCTCTTC ATCAGAAGGA AACGGTCCAC GACAATGACT TTGAACCATA CCTCACTGGC 
CAGTCTAATC CGAATAACAG TTACCAATCC ATGACCGACC CATACCTGTC TAGCTACTAC 
GCCCCCTCAA TTGGGTTCCC GTACCCCCTC AGCGAGGCTC CCTGGTCCAC AGGTGGAGAC 
CCGCCGATCC CATACCTCAC TCCGTATGCC CCCCTCAGCA ATGGAGACCA TCACTTCATG 
CATGACACTG TGTTTGGACA GCCGGGGGGT CTGGGCAGTA GCATTTATCC ACACCGCTTT 
AACTTTTTCC CCGAAAACCC TGCGTTTTCT GCCTGGGGTA CGAGTGGCTC CCAGGGCCAG 
CAGACTCAGA GTTCTGCATA TGGGGGCAGC TACAGTTACC CGCCCAGTTC TTTAGGTGGA 
ACCCTGGTGC CAGACGGGCA AACAGGCTTC CCTAGTGACA CCTTGAATAA GGCACCAGGT 
ATGAATAGCT TGGAGCAGGG CATGGTGGGG CTGAAGATCG GTGGTGATGT GACTGGAGGT 
GGTTCCGGAG TTAAGACGGT GGGCTCCGTG ATTGGAGGAG GGCCGGTGGC TGCGGCGGTG 
GCCACGGGTA ATGGTGGGAC TCCTATTGGA CTGCCGCCCC CTAAGCCCAC ATCTTGGGCA 
GCCATTGCTA GCAAACCGGC CAAGCCTCAG CAACTGAAGG TGAAGAGTAA GCCAGGGATG 
CCCATGGCTG GAGGCACCCT GCCTCCGCCG CCCATCAAAC ACAACATGGA CATTGGCACT 
TGGGATAATA AGGGGCCTGT GACCAAAGTG GCATCATCAT TGCCACCTCA CCACCACCAC 
CAGCAGCAGC CTCCCCTCCA CTCCCAGGGT CACCTTCCAC CTCACCCTCA TGGACTGCCA 
CCCCCACCTC AGCCCCCAAT GCCGTCTGCA CAATCACTTG CCCAGCAGAT GGCCATGCAG 
GGCCCACCCC CTCCGCAGCC TTACCAGAAC CACGCCCCTG CTCCTCCCCC GCAAACTCGG 
TGGGTTGCCC CACGCAATCG CAACCCTGGT TATGGTGGTG GAGGAAGCGT GGACAGCAGT 
GGCTCCTCGA GTGGTGGAGG GGTCGGCAAT GGAGGCGGAG GTGGCCCACC GGGCTCCGGC 
GCCATCGAGT CCCATCCCGT CTTAGAAAAG CTGCGTGCGG CACACAGCTA CAACCCCAAG 
GAGTTCGATT GGAACCTGAA AAATGGCCGG GTGTTCATCA TTAAGAGCTA CTCTGAGGAC 
GACATCCATC GCTCCATCAA GTACTCCATC TGGTGCAGCA CGGAACATGG AAACAAGCGG 
CTGGATTCTG CGTTTCGTGC CATCAACGGC AAAGGTCCTG TCTACCTGCT GTTCAGCGTC 
AACGGCAGCG GACACTTCTG TGGCGTGGCG GAGATGCGTT CGCCTGTTGA CTACGGCACC 
AGTGCCGGTG TTTGGGCGCA GGACAAGTGG AAGGGCAAAT TTGATGTGGA CTGGCTGTTT 
GTTAAAGACG TGCCCAACAG TCAGCTCAGG CACATCCGCC TGGAGAACAA TGACAACAAG 
CCCGTGACCA ACTCGCGTGA CACACAGGAG GTGCCTCTGG AAAAGGCCAA GCAGGTGCTG 
AAGATCATCG CTACCTACAA ACACACAACC TCCATCTTCG ATGACTTCTC ACATTACGAG 
AAACGGCAGG AGGAGGAAGA GGTGGTAAGA AAGAATCTTG AGCCTGCTCC TATCCAGAAC 
CGATCCCGAC TGGATCAAGA ACGCCAGAAC CGAAATAAAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YTHDF1 ( XM_005167004.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167061.1 Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14193
Clone ID Related Accession (Same CDS sequence)	XM_005167004.4 , XM_005167004.3
Accession Version	XM_005167004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	YTH domain-containing family protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCA CCAGTATTGA CCCACAGACA TCAAAAGGGC AAGACGCTAA AGTGCAAAAT 
GGCTCTCTTC ATCAGAAGGA AACGGTCCAC GACAATGACT TTGAACCATA CCTCACTGGC 
CAGTCTAATC CGAATAACAG TTACCAATCC ATGACCGACC CATACCTGTC TAGCTACTAC 
GCCCCCTCAA TTGGGTTCCC GTACCCCCTC AGCGAGGCTC CCTGGTCCAC AGGTGGAGAC 
CCGCCGATCC CATACCTCAC TCCGTATGCC CCCCTCAGCA ATGGAGACCA TCACTTCATG 
CATGACACTG TGTTTGGACA GCCGGGGGGT CTGGGCAGTA GCATTTATCC ACACCGCTTT 
AACTTTTTCC CCGAAAACCC TGCGTTTTCT GCCTGGGGTA CGAGTGGCTC CCAGGGCCAG 
CAGACTCAGA GTTCTGCATA TGGGGGCAGC TACAGTTACC CGCCCAGTTC TTTAGGTGGA 
ACCCTGGTGC CAGACGGGCA AACAGGCTTC CCTAGTGACA CCCTGAATAA GGCACCAGGT 
ATGAATAGCT TGGAGCAGGG CATGGTGGGG CTGAAGATCG GTGGTGATGT GACTGGAGGT 
GGTTCCGGAG TTAAGACGGT GGGCTCCGTG ATTGGAGGAG GGCCGGTGGC TGCGGCGGTG 
GCCACGGGTA ATGGTGGGAC TCCTATTGGA CTGCCGCCCC CTAAGCCCAC ATCTTGGGCA 
GCCATTGCTA GCAAACCGGC CAAGCCTCAG CAACTGAAGG TGAAGAGTAA GCCAGGGATG 
CCCATGGCTG GAGGCACCCT GCCTCCGCCG CCCATCAAAC ACAACATGGA CATTGGCACT 
TGGGATAATA AGGGGCCTGT GACCAAAGTG GCATCATCAT TGCCACCTCA CCACCACCAC 
CAGCAGCAGC CTCCCCTCCA CTCCCAGGGT CACCTTCCAC CTCACCCTCA TGGACTGCCA 
CCCCCACCTC AGCCCCCAAT GCCGTCTGCA CAATCACTTG CCCAGCAGAT GGCCATGCAG 
GGCCCACCCC CTCCGCAGCC TTACCAGAAC CACGCCCCTG CTCCTCCCCC GCAAACTCGG 
TGGGTTGCCC CACGCAATCG CAACCCTGGT TATGGTGGTG GAGGAAGCGT GGACAGCAGT 
GGCTCCTCGA GTGGTGGAGG GGTCGGCAAT GGAGGCGGAG GTGGCCCACC GGGCTCCGGC 
GCCATCGAGT CCCATCCCGT CTTAGAAAAG CTGCGTGCGG CACACAGCTA CAACCCCAAG 
GAGTTCGATT GGAACCTGAA AAATGGCCGG GTGTTCATCA TTAAGAGCTA CTCTGAGGAC 
GACATCCATC GCTCCATCAA GTACTCCATC TGGTGCAGCA CGGAACATGG AAACAAGCGG 
CTGGATTCTG CGTTTCGTGC CATCAACGGC AAAGGTCCTG TCTACCTGCT GTTCAGCGTC 
AACGGCAGCG GACACTTCTG TGGCGTGGCG GAGATGCGTT CGCCTGTTGA CTACGGCACC 
AGTGCCGGTG TTTGGGCGCA GGACAAGTGG AAGGGCAAAT TTGATGTGGA CTGGCTGTTT 
GTTAAAGACG TGCCCAACAG TCAGCTCAGG CACATCCGCC TGGAGAACAA TGACAACAAG 
CCCGTGACCA ACTCGCGTGA CACACAGGAG GTGCCTCTGG AAAAGGCCAA GCAGGTGCTG 
AAGATCATCG CTACCTACAA ACACACAACC TCCATCTTCG ATGACTTCTC ACATTACGAG 
AAACGGCAGG AGGAGGAAGA GGTGAATCTT GAGCCTGCTC CTATCCAGAA CCGATCCCGA 
CTGGATCAAG AACGCCAGAA CCGAAATAAA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167061.1
CDS	401..2236
Translation	MSTTSIDPQTSKGQDAKVQNGSLHQKETVHDNDFEPYLTGQSNPNNSYQSMTDPYLSSYYAPSIGFPYPLSEAPWSTGGDPPIPYLTPYAPLSNGDHHFMHDTVFGQPGGLGSSIYPHRFNFFPENPAFSAWGTSGSQGQQTQSSAYGGSYSYPPSSLGGTLVPDGQTGFPSDTLNKAPGMNSLEQGMVGLKIGGDVTGGGSGVKTVGSVIGGGPVAAAVATGNGGTPIGLPPPKPTSWAAIASKPAKPQQLKVKSKPGMPMAGGTLPPPPIKHNMDIGTWDNKGPVTKVASSLPPHHHHQQQPPLHSQGHLPPHPHGLPPPPQPPMPSAQSLAQQMAMQGPPPPQPYQNHAPAPPPQTRWVAPRNRNPGYGGGGSVDSSGSSSGGGVGNGGGGGPPGSGAIESHPVLEKLRAAHSYNPKEFDWNLKNGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDSAFRAINGKGPVYLLFSVNGSGHFCGVAEMRSPVDYGTSAGVWAQDKWKGKFDVDWLFVKDVPNSQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATYKHTTSIFDDFSHYEKRQEEEEVNLEPAPIQNRSRLDQERQNRNKQ

Target ORF information:

RefSeq Version	XM_005167004.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167004.4

ORF Insert Sequence:

ATGTCTACCA CCAGTATTGA CCCACAGACA TCAAAAGGGC AAGACGCTAA AGTGCAAAAT 
GGCTCTCTTC ATCAGAAGGA AACGGTCCAC GACAATGACT TTGAACCATA CCTCACTGGC 
CAGTCTAATC CGAATAACAG TTACCAATCC ATGACCGACC CATACCTGTC TAGCTACTAC 
GCCCCCTCAA TTGGGTTCCC GTACCCCCTC AGCGAGGCTC CCTGGTCCAC AGGTGGAGAC 
CCGCCGATCC CATACCTCAC TCCGTATGCC CCCCTCAGCA ATGGAGACCA TCACTTCATG 
CATGACACTG TGTTTGGACA GCCGGGGGGT CTGGGCAGTA GCATTTATCC ACACCGCTTT 
AACTTTTTCC CCGAAAACCC TGCGTTTTCT GCCTGGGGTA CGAGTGGCTC CCAGGGCCAG 
CAGACTCAGA GTTCTGCATA TGGGGGCAGC TACAGTTACC CGCCCAGTTC TTTAGGTGGA 
ACCCTGGTGC CAGACGGGCA AACAGGCTTC CCTAGTGACA CCCTGAATAA GGCACCAGGT 
ATGAATAGCT TGGAGCAGGG CATGGTGGGG CTGAAGATCG GTGGTGATGT GACTGGAGGT 
GGTTCCGGAG TTAAGACGGT GGGCTCCGTG ATTGGAGGAG GGCCGGTGGC TGCGGCGGTG 
GCCACGGGTA ATGGTGGGAC TCCTATTGGA CTGCCGCCCC CTAAGCCCAC ATCTTGGGCA 
GCCATTGCTA GCAAACCGGC CAAGCCTCAG CAACTGAAGG TGAAGAGTAA GCCAGGGATG 
CCCATGGCTG GAGGCACCCT GCCTCCGCCG CCCATCAAAC ACAACATGGA CATTGGCACT 
TGGGATAATA AGGGGCCTGT GACCAAAGTG GCATCATCAT TGCCACCTCA CCACCACCAC 
CAGCAGCAGC CTCCCCTCCA CTCCCAGGGT CACCTTCCAC CTCACCCTCA TGGACTGCCA 
CCCCCACCTC AGCCCCCAAT GCCGTCTGCA CAATCACTTG CCCAGCAGAT GGCCATGCAG 
GGCCCACCCC CTCCGCAGCC TTACCAGAAC CACGCCCCTG CTCCTCCCCC GCAAACTCGG 
TGGGTTGCCC CACGCAATCG CAACCCTGGT TATGGTGGTG GAGGAAGCGT GGACAGCAGT 
GGCTCCTCGA GTGGTGGAGG GGTCGGCAAT GGAGGCGGAG GTGGCCCACC GGGCTCCGGC 
GCCATCGAGT CCCATCCCGT CTTAGAAAAG CTGCGTGCGG CACACAGCTA CAACCCCAAG 
GAGTTCGATT GGAACCTGAA AAATGGCCGG GTGTTCATCA TTAAGAGCTA CTCTGAGGAC 
GACATCCATC GCTCCATCAA GTACTCCATC TGGTGCAGCA CGGAACATGG AAACAAGCGG 
CTGGATTCTG CGTTTCGTGC CATCAACGGC AAAGGTCCTG TCTACCTGCT GTTCAGCGTC 
AACGGCAGCG GACACTTCTG TGGCGTGGCG GAGATGCGTT CGCCTGTTGA CTACGGCACC 
AGTGCCGGTG TTTGGGCGCA GGACAAGTGG AAGGGCAAAT TTGATGTGGA CTGGCTGTTT 
GTTAAAGACG TGCCCAACAG TCAGCTCAGG CACATCCGCC TGGAGAACAA TGACAACAAG 
CCCGTGACCA ACTCGCGTGA CACACAGGAG GTGCCTCTGG AAAAGGCCAA GCAGGTGCTG 
AAGATCATCG CTACCTACAA ACACACAACC TCCATCTTCG ATGACTTCTC ACATTACGAG 
AAACGGCAGG AGGAGGAAGA GGTGAATCTT GAGCCTGCTC CTATCCAGAA CCGATCCCGA 
CTGGATCAAG AACGCCAGAA CCGAAATAAA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YTHDF1 ( XM_005167004.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167061.1 Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14193
Clone ID Related Accession (Same CDS sequence)	XM_005167004.4 , XM_005167004.3
Accession Version	XM_005167004.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	YTH domain-containing family protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCA CCAGTATTGA CCCACAGACA TCAAAAGGGC AAGACGCTAA AGTGCAAAAT 
GGCTCTCTTC ATCAGAAGGA AACGGTCCAC GACAATGACT TTGAACCATA CCTCACTGGC 
CAGTCTAATC CGAATAACAG TTACCAATCC ATGACCGACC CATACCTGTC TAGCTACTAC 
GCCCCCTCAA TTGGGTTCCC GTACCCCCTC AGCGAGGCTC CCTGGTCCAC AGGTGGAGAC 
CCGCCGATCC CATACCTCAC TCCGTATGCC CCCCTCAGCA ATGGAGACCA TCACTTCATG 
CATGACACTG TGTTTGGACA GCCGGGGGGT CTGGGCAGTA GCATTTATCC ACACCGCTTT 
AACTTTTTCC CCGAAAACCC TGCGTTTTCT GCCTGGGGTA CGAGTGGCTC CCAGGGCCAG 
CAGACTCAGA GTTCTGCATA TGGGGGCAGC TACAGTTACC CGCCCAGTTC TTTAGGTGGA 
ACCCTGGTGC CAGACGGGCA AACAGGCTTC CCTAGTGACA CCCTGAATAA GGCACCAGGT 
ATGAATAGCT TGGAGCAGGG CATGGTGGGG CTGAAGATCG GTGGTGATGT GACTGGAGGT 
GGTTCCGGAG TTAAGACGGT GGGCTCCGTG ATTGGAGGAG GGCCGGTGGC TGCGGCGGTG 
GCCACGGGTA ATGGTGGGAC TCCTATTGGA CTGCCGCCCC CTAAGCCCAC ATCTTGGGCA 
GCCATTGCTA GCAAACCGGC CAAGCCTCAG CAACTGAAGG TGAAGAGTAA GCCAGGGATG 
CCCATGGCTG GAGGCACCCT GCCTCCGCCG CCCATCAAAC ACAACATGGA CATTGGCACT 
TGGGATAATA AGGGGCCTGT GACCAAAGTG GCATCATCAT TGCCACCTCA CCACCACCAC 
CAGCAGCAGC CTCCCCTCCA CTCCCAGGGT CACCTTCCAC CTCACCCTCA TGGACTGCCA 
CCCCCACCTC AGCCCCCAAT GCCGTCTGCA CAATCACTTG CCCAGCAGAT GGCCATGCAG 
GGCCCACCCC CTCCGCAGCC TTACCAGAAC CACGCCCCTG CTCCTCCCCC GCAAACTCGG 
TGGGTTGCCC CACGCAATCG CAACCCTGGT TATGGTGGTG GAGGAAGCGT GGACAGCAGT 
GGCTCCTCGA GTGGTGGAGG GGTCGGCAAT GGAGGCGGAG GTGGCCCACC GGGCTCCGGC 
GCCATCGAGT CCCATCCCGT CTTAGAAAAG CTGCGTGCGG CACACAGCTA CAACCCCAAG 
GAGTTCGATT GGAACCTGAA AAATGGCCGG GTGTTCATCA TTAAGAGCTA CTCTGAGGAC 
GACATCCATC GCTCCATCAA GTACTCCATC TGGTGCAGCA CGGAACATGG AAACAAGCGG 
CTGGATTCTG CGTTTCGTGC CATCAACGGC AAAGGTCCTG TCTACCTGCT GTTCAGCGTC 
AACGGCAGCG GACACTTCTG TGGCGTGGCG GAGATGCGTT CGCCTGTTGA CTACGGCACC 
AGTGCCGGTG TTTGGGCGCA GGACAAGTGG AAGGGCAAAT TTGATGTGGA CTGGCTGTTT 
GTTAAAGACG TGCCCAACAG TCAGCTCAGG CACATCCGCC TGGAGAACAA TGACAACAAG 
CCCGTGACCA ACTCGCGTGA CACACAGGAG GTGCCTCTGG AAAAGGCCAA GCAGGTGCTG 
AAGATCATCG CTACCTACAA ACACACAACC TCCATCTTCG ATGACTTCTC ACATTACGAG 
AAACGGCAGG AGGAGGAAGA GGTGAATCTT GAGCCTGCTC CTATCCAGAA CCGATCCCGA 
CTGGATCAAG AACGCCAGAA CCGAAATAAA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167061.1
CDS	410..2245
Translation	MSTTSIDPQTSKGQDAKVQNGSLHQKETVHDNDFEPYLTGQSNPNNSYQSMTDPYLSSYYAPSIGFPYPLSEAPWSTGGDPPIPYLTPYAPLSNGDHHFMHDTVFGQPGGLGSSIYPHRFNFFPENPAFSAWGTSGSQGQQTQSSAYGGSYSYPPSSLGGTLVPDGQTGFPSDTLNKAPGMNSLEQGMVGLKIGGDVTGGGSGVKTVGSVIGGGPVAAAVATGNGGTPIGLPPPKPTSWAAIASKPAKPQQLKVKSKPGMPMAGGTLPPPPIKHNMDIGTWDNKGPVTKVASSLPPHHHHQQQPPLHSQGHLPPHPHGLPPPPQPPMPSAQSLAQQMAMQGPPPPQPYQNHAPAPPPQTRWVAPRNRNPGYGGGGSVDSSGSSSGGGVGNGGGGGPPGSGAIESHPVLEKLRAAHSYNPKEFDWNLKNGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDSAFRAINGKGPVYLLFSVNGSGHFCGVAEMRSPVDYGTSAGVWAQDKWKGKFDVDWLFVKDVPNSQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATYKHTTSIFDDFSHYEKRQEEEEVNLEPAPIQNRSRLDQERQNRNKQ

Target ORF information:

RefSeq Version	XM_005167004.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio YTH N(6)-methyladenosine RNA binding protein 1 (ythdf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167004.3

ORF Insert Sequence:

ATGTCTACCA CCAGTATTGA CCCACAGACA TCAAAAGGGC AAGACGCTAA AGTGCAAAAT 
GGCTCTCTTC ATCAGAAGGA AACGGTCCAC GACAATGACT TTGAACCATA CCTCACTGGC 
CAGTCTAATC CGAATAACAG TTACCAATCC ATGACCGACC CATACCTGTC TAGCTACTAC 
GCCCCCTCAA TTGGGTTCCC GTACCCCCTC AGCGAGGCTC CCTGGTCCAC AGGTGGAGAC 
CCGCCGATCC CATACCTCAC TCCGTATGCC CCCCTCAGCA ATGGAGACCA TCACTTCATG 
CATGACACTG TGTTTGGACA GCCGGGGGGT CTGGGCAGTA GCATTTATCC ACACCGCTTT 
AACTTTTTCC CCGAAAACCC TGCGTTTTCT GCCTGGGGTA CGAGTGGCTC CCAGGGCCAG 
CAGACTCAGA GTTCTGCATA TGGGGGCAGC TACAGTTACC CGCCCAGTTC TTTAGGTGGA 
ACCCTGGTGC CAGACGGGCA AACAGGCTTC CCTAGTGACA CCCTGAATAA GGCACCAGGT 
ATGAATAGCT TGGAGCAGGG CATGGTGGGG CTGAAGATCG GTGGTGATGT GACTGGAGGT 
GGTTCCGGAG TTAAGACGGT GGGCTCCGTG ATTGGAGGAG GGCCGGTGGC TGCGGCGGTG 
GCCACGGGTA ATGGTGGGAC TCCTATTGGA CTGCCGCCCC CTAAGCCCAC ATCTTGGGCA 
GCCATTGCTA GCAAACCGGC CAAGCCTCAG CAACTGAAGG TGAAGAGTAA GCCAGGGATG 
CCCATGGCTG GAGGCACCCT GCCTCCGCCG CCCATCAAAC ACAACATGGA CATTGGCACT 
TGGGATAATA AGGGGCCTGT GACCAAAGTG GCATCATCAT TGCCACCTCA CCACCACCAC 
CAGCAGCAGC CTCCCCTCCA CTCCCAGGGT CACCTTCCAC CTCACCCTCA TGGACTGCCA 
CCCCCACCTC AGCCCCCAAT GCCGTCTGCA CAATCACTTG CCCAGCAGAT GGCCATGCAG 
GGCCCACCCC CTCCGCAGCC TTACCAGAAC CACGCCCCTG CTCCTCCCCC GCAAACTCGG 
TGGGTTGCCC CACGCAATCG CAACCCTGGT TATGGTGGTG GAGGAAGCGT GGACAGCAGT 
GGCTCCTCGA GTGGTGGAGG GGTCGGCAAT GGAGGCGGAG GTGGCCCACC GGGCTCCGGC 
GCCATCGAGT CCCATCCCGT CTTAGAAAAG CTGCGTGCGG CACACAGCTA CAACCCCAAG 
GAGTTCGATT GGAACCTGAA AAATGGCCGG GTGTTCATCA TTAAGAGCTA CTCTGAGGAC 
GACATCCATC GCTCCATCAA GTACTCCATC TGGTGCAGCA CGGAACATGG AAACAAGCGG 
CTGGATTCTG CGTTTCGTGC CATCAACGGC AAAGGTCCTG TCTACCTGCT GTTCAGCGTC 
AACGGCAGCG GACACTTCTG TGGCGTGGCG GAGATGCGTT CGCCTGTTGA CTACGGCACC 
AGTGCCGGTG TTTGGGCGCA GGACAAGTGG AAGGGCAAAT TTGATGTGGA CTGGCTGTTT 
GTTAAAGACG TGCCCAACAG TCAGCTCAGG CACATCCGCC TGGAGAACAA TGACAACAAG 
CCCGTGACCA ACTCGCGTGA CACACAGGAG GTGCCTCTGG AAAAGGCCAA GCAGGTGCTG 
AAGATCATCG CTACCTACAA ACACACAACC TCCATCTTCG ATGACTTCTC ACATTACGAG 
AAACGGCAGG AGGAGGAAGA GGTGAATCTT GAGCCTGCTC CTATCCAGAA CCGATCCCGA 
CTGGATCAAG AACGCCAGAA CCGAAATAAA CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Genome research27(7)1184-1194(2017 Jul)
Despic V,Dejung M,Gu M,Krishnan J,Zhang J,Herzel L,Straube K,Gerstein MB,Butter F,Neugebauer KM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Concomitant duplications of opioid peptide and receptor genes before the origin of jawed vertebrates.
PloS one5(5)e10512(2010 May)
Sundstr?m G,Dreborg S,Larhammar D

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

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YTHDF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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