src cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	src
Entrez Gene ID	325084
Full Name	v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
Synonyms	c-src,fc54g04,p60-Src,sb:cb864,wu:fc54g04
General protein information	Preferred Names v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog Names proto-oncogene tyrosine-protein kinase Src pp60c-src protein-tyrosine kinase proto-oncogene c-Src v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353519.1	XP_017209008.1	proto-oncogene tyrosine-protein kinase Src isoform X1
NM_001003837.2	NP_001003837.2	proto-oncogene tyrosine-protein kinase Src
XM_017353520.1	XP_017209009.1	proto-oncogene tyrosine-protein kinase Src isoform X2
XM_005174130.4	XP_005174187.1	proto-oncogene tyrosine-protein kinase Src isoform X3
XM_005174130.3	XP_005174187.1	proto-oncogene tyrosine-protein kinase Src isoform X3
XM_017353519.2	XP_017209008.1	proto-oncogene tyrosine-protein kinase Src isoform X1
XM_017353520.2	XP_017209009.1	proto-oncogene tyrosine-protein kinase Src isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRC	NP_005408.1
Danio rerio (zebrafish)	src	NP_001003837.2
Gallus gallus (chicken)	SRC	NP_990788.2
Canis lupus familiaris (dog)	SRC	XP_005635095.1
Drosophila melanogaster (fruit fly)	Src64B	NP_001014563.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006510	XP_316537.2
Pan troglodytes (chimpanzee)	SRC	XP_001141228.2
	SRC	XP_001092301.1
Rattus norvegicus (Norway rat)	Src	NP_114183.1
Bos taurus (cattle)	SRC	NP_001104274.1
Mus musculus (house mouse)	Src	NP_033297.2
Xenopus tropicalis (tropical clawed frog)	src	NP_001011413.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A dominant gain-of-function mutation in universal tyrosine kinase SRC causes thrombocytopenia, myelofibrosis, bleeding, and bone pathologies.
Turro E, Greene D, Wijgaerts A, Thys C, Lentaigne C, Bariana TK, Westbury SK, Kelly AM, Selleslag D, Stephens JC, Papadia S, Simeoni I, Penkett CJ, Ashford S, Attwood A, Austin S, Bakchoul T, Collins P, Deevi SV, Favier R, Kostadima M, Lambert MP, Mathias M, Millar CM, Peerlinck K, Perry DJ, Schulman S, Whitehorn D, Wittevrongel C, , De Maeyer M, Rendon A, Gomez K, Erber WN, Mumford AD, Nurden P, Stirrups K, Bradley JR, Raymond FL, Laffan MA, Van Geet C, Richardson S, Freson K, Ouwehand WH
Science translational medicine8(328)328ra30(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Src-Tks5 pathway is required for neural crest cell migration during embryonic development
Title: A Src-Tks5 pathway is required for neural crest cell migration during embryonic development.

The following src gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the src cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02876	NM_001003837.2 Latest version!	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa28045	XM_005174130.4 Latest version!	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa57340	XM_017353519.2 Latest version!	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa57341	XM_017353520.2 Latest version!	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa28045	XM_005174130.3	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa57340	XM_017353519.1	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa57341	XM_017353520.1	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

src ( NM_001003837.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003837.2 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), mRNA.

Price & Availability↑

CloneID	ODa02876
Clone ID Related Accession (Same CDS sequence)	NM_001003837.2
Accession Version	NM_001003837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC116544.1. On May 21, 2006 this sequence version replaced NM_001003837.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116544.1, AJ620750.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGGAG CCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAT 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCAGTAG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATCAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 
CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 
TCTGACTCCA TCCAGGCTGA AGAATGGTAC TTTGGAAAAA TCACACGCAG AGACTCTGAG 
CGACTGCTCT TGAATATGGA GAACAGACGA GGAACTTTCC TGGTCAGAGA GAGCGAGACC 
ACCAAAGGTG CCTACTGTCT CTCCGTCCTG GATTATGACA ACGTGAAGGG CCTGAATGTG 
AAACATTATA AGATCCGCAA GCTGGACAGC GGTGGATTTT ACATCACGTC CCGCACACAG 
TTCAGCACGT TACAGCAGCT GGTCAACCAC TACCGCCAGC ACGCAGACGG CCTGTGCCAC 
TCCCTGACTG ACGTGTGTCC AGTGCTGAAG CCTCCGACTC AGGGTCTGGC ACGTGATGCC 
TGGGAGATTC CTCGAGACTC GCTGAGACTG GATGTCAAGC TGGGCCAGGG CTGCTTCGGG 
GAGGTCTGGA TGGGCACCTG GAACGGGACG ACACGTGTGG CCATCAAGAC TCTGAAGCCT 
GGCACCATGT CTCCCGAAGC GTTCCTGCAG GAGGCGCAAG TGATGAAGAA GCTCCGGCAT 
GAGAAGCTGG TGCAGCTTTA CGCCGTCGTC TCAGAGGAGC CCATTTACAT CGTCACTGAG 
TACATGGGTC AAGGAAGCCT GCTGGATTTC CTGAAAGGTG ACATGGGGAA AATGCTGCGT 
TTGCCTCAGC TCGTGGACAT GGCCTCTCAG ATCGCCTCAG GAATGGCATA CGTGGAGAGA 
ATGAACTATG TGCACAGGGA CCTCAGGGCT GCCAACATCC TGGTGGGCGA TAACCTGGTG 
TGTAAAGTGG CTGATTTCGG CCTCGCTCGA CTCATTGAGG ACAACGAATA CACTGCGAGA 
CAAGGAGCAA AGTTTCCTAT TAAGTGGACT GCACCGGAGG CGGCTCTGTA TGGACGCTTC 
ACGATCAAAT CTGACGTGTG GTCGTTTGGG ATTTTACTGA CGGAGCTCAC CACCAAAGGC 
AGGGTGCCTT ATCCAGGTAT GGTGAACCGC GAGGTGCTTG ACCAGGTGGA GCGTGGCTAC 
AGGATGCCCT GCCCAGCCGA GTGCCCCGAC TCCCTGCATG AGCTCATGCT GACCTGCTGG 
CGCAAAGAGC CCGAAGAGCG GCCCACATTC GAGTACCTGC AGGGTTTCCT GGAGGACTAC 
TTCACCTCCA CCGAACCACA ATACCAACCC GGAGAAAACC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003837.2
CDS	396..2000
Translation	MGGAKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTEGDWWLARSLTTGESGYIPSNYVAPSDSIQAEEWYFGKITRRDSERLLLNMENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	NM_001003837.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003837.2

ORF Insert Sequence:

ATGGGTGGAG CCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAT 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCAGTAG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATCAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 
CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 
TCTGACTCCA TCCAGGCTGA AGAATGGTAC TTTGGAAAAA TCACACGCAG AGACTCTGAG 
CGACTGCTCT TGAATATGGA GAACAGACGA GGAACTTTCC TGGTCAGAGA GAGCGAGACC 
ACCAAAGGTG CCTACTGTCT CTCCGTCCTG GATTATGACA ACGTGAAGGG CCTGAATGTG 
AAACATTATA AGATCCGCAA GCTGGACAGC GGTGGATTTT ACATCACGTC CCGCACACAG 
TTCAGCACGT TACAGCAGCT GGTCAACCAC TACCGCCAGC ACGCAGACGG CCTGTGCCAC 
TCCCTGACTG ACGTGTGTCC AGTGCTGAAG CCTCCGACTC AGGGTCTGGC ACGTGATGCC 
TGGGAGATTC CTCGAGACTC GCTGAGACTG GATGTCAAGC TGGGCCAGGG CTGCTTCGGG 
GAGGTCTGGA TGGGCACCTG GAACGGGACG ACACGTGTGG CCATCAAGAC TCTGAAGCCT 
GGCACCATGT CTCCCGAAGC GTTCCTGCAG GAGGCGCAAG TGATGAAGAA GCTCCGGCAT 
GAGAAGCTGG TGCAGCTTTA CGCCGTCGTC TCAGAGGAGC CCATTTACAT CGTCACTGAG 
TACATGGGTC AAGGAAGCCT GCTGGATTTC CTGAAAGGTG ACATGGGGAA AATGCTGCGT 
TTGCCTCAGC TCGTGGACAT GGCCTCTCAG ATCGCCTCAG GAATGGCATA CGTGGAGAGA 
ATGAACTATG TGCACAGGGA CCTCAGGGCT GCCAACATCC TGGTGGGCGA TAACCTGGTG 
TGTAAAGTGG CTGATTTCGG CCTCGCTCGA CTCATTGAGG ACAACGAATA CACTGCGAGA 
CAAGGAGCAA AGTTTCCTAT TAAGTGGACT GCACCGGAGG CGGCTCTGTA TGGACGCTTC 
ACGATCAAAT CTGACGTGTG GTCGTTTGGG ATTTTACTGA CGGAGCTCAC CACCAAAGGC 
AGGGTGCCTT ATCCAGGTAT GGTGAACCGC GAGGTGCTTG ACCAGGTGGA GCGTGGCTAC 
AGGATGCCCT GCCCAGCCGA GTGCCCCGAC TCCCTGCATG AGCTCATGCT GACCTGCTGG 
CGCAAAGAGC CCGAAGAGCG GCCCACATTC GAGTACCTGC AGGGTTTCCT GGAGGACTAC 
TTCACCTCCA CCGAACCACA ATACCAACCC GGAGAAAACC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

src ( XM_005174130.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174187.1 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa28045
Clone ID Related Accession (Same CDS sequence)	XM_005174130.4 , XM_005174130.3
Accession Version	XM_005174130.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174130.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG AATGGTACTT CGGAAAAATC 
ACACGCAGAG ACTCTGAGCG ACTGCTCTTG AATCTGGAGA ACAGACGAGG AACTTTCCTG 
GTCAGAGAGA GCGAGACCAC CAAAGGTGCC TACTGTCTCT CCGTCCTGGA TTACGACAAC 
GTGAAGGGCC TGAATGTAAA ACATTATAAG ATCCGCAAAC TGGACAGCGG TGGATTTTAC 
ATCACGTCCC GCACACAGTT CAGCACATTA CAGCAGCTGG TCAACCACTA CCGCCAGCAC 
GCAGACGGCC TGTGCCACTC CCTGACTGAC GTGTGTCCAG TGCTGAAGCC TCCGACTCAG 
GGTCTGGCAC GTGATGCCTG GGAGATTCCT CGAGACTCGC TGAGACTGGA TGTCAAGCTG 
GGCCAGGGCT GCTTCGGGGA GGTCTGGATG GGCACCTGGA ACGGGACGAC ACGTGTGGCC 
ATCAAGACTC TGAAGCCTGG CACCATGTCT CCCGAAGCGT TCCTGCAGGA GGCGCAAGTG 
ATGAAGAAGC TCCGGCATGA GAAGCTGGTG CAGCTTTACG CCGTCGTCTC AGAGGAGCCC 
ATTTACATAG TCACTGAGTA CATGGGTCAA GGAAGCCTGC TGGATTTCCT GAAAGGTGAC 
ATGGGGAAAA TGCTGCGTTT GCCTCAGCTC GTGGACATGG CCTCTCAGAT CGCCTCAGGA 
ATGGCATACG TGGAGAGAAT GAACTATGTG CACAGGGACC TCAGGGCTGC CAACATCCTG 
GTGGGCGATA ACCTGGTGTG TAAAGTGGCT GATTTCGGCC TCGCTCGACT CATTGAGGAC 
AACGAATACA CCGCGAGACA AGGAGCAAAG TTTCCTATTA AGTGGACTGC ACCGGAGGCA 
GCTCTGTACG GACGCTTCAC CATCAAATCT GACGTGTGGT CGTTTGGGAT TTTACTGACG 
GAGCTCACCA CCAAAGGCAG GGTGCCTTAT CCAGGTATGG TGAACCGCGA GGTGCTTGAC 
CAGGTGGAGC GTGGCTACAG GATGCCCTGC CCGGCCGAAT GCCCTGACTC CCTGCACGAG 
CTCATGCTGA CCTGCTGGCG CAAAGAGCCC GAAGAGCGGC CCACATTCGA GTACCTGCAG 
GGTTTCCTGG AGGACTACTT CACCTCCACC GAACCACAAT ACCAACCCGG AGAAAACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174187.1
CDS	436..2058
Translation	MGGVKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTRKLNPREGDWWLARSLTTGESGYIPSNYVAPSDSIQAEEWYFGKITRRDSERLLLNLENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	XM_005174130.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174130.4

ORF Insert Sequence:

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG AATGGTACTT CGGAAAAATC 
ACACGCAGAG ACTCTGAGCG ACTGCTCTTG AATCTGGAGA ACAGACGAGG AACTTTCCTG 
GTCAGAGAGA GCGAGACCAC CAAAGGTGCC TACTGTCTCT CCGTCCTGGA TTACGACAAC 
GTGAAGGGCC TGAATGTAAA ACATTATAAG ATCCGCAAAC TGGACAGCGG TGGATTTTAC 
ATCACGTCCC GCACACAGTT CAGCACATTA CAGCAGCTGG TCAACCACTA CCGCCAGCAC 
GCAGACGGCC TGTGCCACTC CCTGACTGAC GTGTGTCCAG TGCTGAAGCC TCCGACTCAG 
GGTCTGGCAC GTGATGCCTG GGAGATTCCT CGAGACTCGC TGAGACTGGA TGTCAAGCTG 
GGCCAGGGCT GCTTCGGGGA GGTCTGGATG GGCACCTGGA ACGGGACGAC ACGTGTGGCC 
ATCAAGACTC TGAAGCCTGG CACCATGTCT CCCGAAGCGT TCCTGCAGGA GGCGCAAGTG 
ATGAAGAAGC TCCGGCATGA GAAGCTGGTG CAGCTTTACG CCGTCGTCTC AGAGGAGCCC 
ATTTACATAG TCACTGAGTA CATGGGTCAA GGAAGCCTGC TGGATTTCCT GAAAGGTGAC 
ATGGGGAAAA TGCTGCGTTT GCCTCAGCTC GTGGACATGG CCTCTCAGAT CGCCTCAGGA 
ATGGCATACG TGGAGAGAAT GAACTATGTG CACAGGGACC TCAGGGCTGC CAACATCCTG 
GTGGGCGATA ACCTGGTGTG TAAAGTGGCT GATTTCGGCC TCGCTCGACT CATTGAGGAC 
AACGAATACA CCGCGAGACA AGGAGCAAAG TTTCCTATTA AGTGGACTGC ACCGGAGGCA 
GCTCTGTACG GACGCTTCAC CATCAAATCT GACGTGTGGT CGTTTGGGAT TTTACTGACG 
GAGCTCACCA CCAAAGGCAG GGTGCCTTAT CCAGGTATGG TGAACCGCGA GGTGCTTGAC 
CAGGTGGAGC GTGGCTACAG GATGCCCTGC CCGGCCGAAT GCCCTGACTC CCTGCACGAG 
CTCATGCTGA CCTGCTGGCG CAAAGAGCCC GAAGAGCGGC CCACATTCGA GTACCTGCAG 
GGTTTCCTGG AGGACTACTT CACCTCCACC GAACCACAAT ACCAACCCGG AGAAAACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

src ( XM_005174130.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174187.1 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa28045
Clone ID Related Accession (Same CDS sequence)	XM_005174130.4 , XM_005174130.3
Accession Version	XM_005174130.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG AATGGTACTT CGGAAAAATC 
ACACGCAGAG ACTCTGAGCG ACTGCTCTTG AATCTGGAGA ACAGACGAGG AACTTTCCTG 
GTCAGAGAGA GCGAGACCAC CAAAGGTGCC TACTGTCTCT CCGTCCTGGA TTACGACAAC 
GTGAAGGGCC TGAATGTAAA ACATTATAAG ATCCGCAAAC TGGACAGCGG TGGATTTTAC 
ATCACGTCCC GCACACAGTT CAGCACATTA CAGCAGCTGG TCAACCACTA CCGCCAGCAC 
GCAGACGGCC TGTGCCACTC CCTGACTGAC GTGTGTCCAG TGCTGAAGCC TCCGACTCAG 
GGTCTGGCAC GTGATGCCTG GGAGATTCCT CGAGACTCGC TGAGACTGGA TGTCAAGCTG 
GGCCAGGGCT GCTTCGGGGA GGTCTGGATG GGCACCTGGA ACGGGACGAC ACGTGTGGCC 
ATCAAGACTC TGAAGCCTGG CACCATGTCT CCCGAAGCGT TCCTGCAGGA GGCGCAAGTG 
ATGAAGAAGC TCCGGCATGA GAAGCTGGTG CAGCTTTACG CCGTCGTCTC AGAGGAGCCC 
ATTTACATAG TCACTGAGTA CATGGGTCAA GGAAGCCTGC TGGATTTCCT GAAAGGTGAC 
ATGGGGAAAA TGCTGCGTTT GCCTCAGCTC GTGGACATGG CCTCTCAGAT CGCCTCAGGA 
ATGGCATACG TGGAGAGAAT GAACTATGTG CACAGGGACC TCAGGGCTGC CAACATCCTG 
GTGGGCGATA ACCTGGTGTG TAAAGTGGCT GATTTCGGCC TCGCTCGACT CATTGAGGAC 
AACGAATACA CCGCGAGACA AGGAGCAAAG TTTCCTATTA AGTGGACTGC ACCGGAGGCA 
GCTCTGTACG GACGCTTCAC CATCAAATCT GACGTGTGGT CGTTTGGGAT TTTACTGACG 
GAGCTCACCA CCAAAGGCAG GGTGCCTTAT CCAGGTATGG TGAACCGCGA GGTGCTTGAC 
CAGGTGGAGC GTGGCTACAG GATGCCCTGC CCGGCCGAAT GCCCTGACTC CCTGCACGAG 
CTCATGCTGA CCTGCTGGCG CAAAGAGCCC GAAGAGCGGC CCACATTCGA GTACCTGCAG 
GGTTTCCTGG AGGACTACTT CACCTCCACC GAACCACAAT ACCAACCCGG AGAAAACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174187.1
CDS	438..2060
Translation	MGGVKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTRKLNPREGDWWLARSLTTGESGYIPSNYVAPSDSIQAEEWYFGKITRRDSERLLLNLENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	XM_005174130.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174130.3

ORF Insert Sequence:

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG AATGGTACTT CGGAAAAATC 
ACACGCAGAG ACTCTGAGCG ACTGCTCTTG AATCTGGAGA ACAGACGAGG AACTTTCCTG 
GTCAGAGAGA GCGAGACCAC CAAAGGTGCC TACTGTCTCT CCGTCCTGGA TTACGACAAC 
GTGAAGGGCC TGAATGTAAA ACATTATAAG ATCCGCAAAC TGGACAGCGG TGGATTTTAC 
ATCACGTCCC GCACACAGTT CAGCACATTA CAGCAGCTGG TCAACCACTA CCGCCAGCAC 
GCAGACGGCC TGTGCCACTC CCTGACTGAC GTGTGTCCAG TGCTGAAGCC TCCGACTCAG 
GGTCTGGCAC GTGATGCCTG GGAGATTCCT CGAGACTCGC TGAGACTGGA TGTCAAGCTG 
GGCCAGGGCT GCTTCGGGGA GGTCTGGATG GGCACCTGGA ACGGGACGAC ACGTGTGGCC 
ATCAAGACTC TGAAGCCTGG CACCATGTCT CCCGAAGCGT TCCTGCAGGA GGCGCAAGTG 
ATGAAGAAGC TCCGGCATGA GAAGCTGGTG CAGCTTTACG CCGTCGTCTC AGAGGAGCCC 
ATTTACATAG TCACTGAGTA CATGGGTCAA GGAAGCCTGC TGGATTTCCT GAAAGGTGAC 
ATGGGGAAAA TGCTGCGTTT GCCTCAGCTC GTGGACATGG CCTCTCAGAT CGCCTCAGGA 
ATGGCATACG TGGAGAGAAT GAACTATGTG CACAGGGACC TCAGGGCTGC CAACATCCTG 
GTGGGCGATA ACCTGGTGTG TAAAGTGGCT GATTTCGGCC TCGCTCGACT CATTGAGGAC 
AACGAATACA CCGCGAGACA AGGAGCAAAG TTTCCTATTA AGTGGACTGC ACCGGAGGCA 
GCTCTGTACG GACGCTTCAC CATCAAATCT GACGTGTGGT CGTTTGGGAT TTTACTGACG 
GAGCTCACCA CCAAAGGCAG GGTGCCTTAT CCAGGTATGG TGAACCGCGA GGTGCTTGAC 
CAGGTGGAGC GTGGCTACAG GATGCCCTGC CCGGCCGAAT GCCCTGACTC CCTGCACGAG 
CTCATGCTGA CCTGCTGGCG CAAAGAGCCC GAAGAGCGGC CCACATTCGA GTACCTGCAG 
GGTTTCCTGG AGGACTACTT CACCTCCACC GAACCACAAT ACCAACCCGG AGAAAACCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

src ( XM_017353519.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209008.1 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57340
Clone ID Related Accession (Same CDS sequence)	XM_017353519.1 , XM_017353519.2
Accession Version	XM_017353519.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG ATCATCTCAG ATTGACTCTG 
GAGAAAAGAT GGTACTTCGG AAAAATCACA CGCAGAGACT CTGAGCGACT GCTCTTGAAT 
CTGGAGAACA GACGAGGAAC TTTCCTGGTC AGAGAGAGCG AGACCACCAA AGGTGCCTAC 
TGTCTCTCCG TCCTGGATTA CGACAACGTG AAGGGCCTGA ATGTAAAACA TTATAAGATC 
CGCAAACTGG ACAGCGGTGG ATTTTACATC ACGTCCCGCA CACAGTTCAG CACATTACAG 
CAGCTGGTCA ACCACTACCG CCAGCACGCA GACGGCCTGT GCCACTCCCT GACTGACGTG 
TGTCCAGTGC TGAAGCCTCC GACTCAGGGT CTGGCACGTG ATGCCTGGGA GATTCCTCGA 
GACTCGCTGA GACTGGATGT CAAGCTGGGC CAGGGCTGCT TCGGGGAGGT CTGGATGGGC 
ACCTGGAACG GGACGACACG TGTGGCCATC AAGACTCTGA AGCCTGGCAC CATGTCTCCC 
GAAGCGTTCC TGCAGGAGGC GCAAGTGATG AAGAAGCTCC GGCATGAGAA GCTGGTGCAG 
CTTTACGCCG TCGTCTCAGA GGAGCCCATT TACATAGTCA CTGAGTACAT GGGTCAAGGA 
AGCCTGCTGG ATTTCCTGAA AGGTGACATG GGGAAAATGC TGCGTTTGCC TCAGCTCGTG 
GACATGGCCT CTCAGATCGC CTCAGGAATG GCATACGTGG AGAGAATGAA CTATGTGCAC 
AGGGACCTCA GGGCTGCCAA CATCCTGGTG GGCGATAACC TGGTGTGTAA AGTGGCTGAT 
TTCGGCCTCG CTCGACTCAT TGAGGACAAC GAATACACCG CGAGACAAGG AGCAAAGTTT 
CCTATTAAGT GGACTGCACC GGAGGCAGCT CTGTACGGAC GCTTCACCAT CAAATCTGAC 
GTGTGGTCGT TTGGGATTTT ACTGACGGAG CTCACCACCA AAGGCAGGGT GCCTTATCCA 
GGTATGGTGA ACCGCGAGGT GCTTGACCAG GTGGAGCGTG GCTACAGGAT GCCCTGCCCG 
GCCGAATGCC CTGACTCCCT GCACGAGCTC ATGCTGACCT GCTGGCGCAA AGAGCCCGAA 
GAGCGGCCCA CATTCGAGTA CCTGCAGGGT TTCCTGGAGG ACTACTTCAC CTCCACCGAA 
CCACAATACC AACCCGGAGA AAACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209008.1
CDS	438..2087
Translation	MGGVKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTRKLNPREGDWWLARSLTTGESGYIPSNYVAPSDSIQAEDHLRLTLEKRWYFGKITRRDSERLLLNLENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	XM_017353519.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353519.1

ORF Insert Sequence:

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG ATCATCTCAG ATTGACTCTG 
GAGAAAAGAT GGTACTTCGG AAAAATCACA CGCAGAGACT CTGAGCGACT GCTCTTGAAT 
CTGGAGAACA GACGAGGAAC TTTCCTGGTC AGAGAGAGCG AGACCACCAA AGGTGCCTAC 
TGTCTCTCCG TCCTGGATTA CGACAACGTG AAGGGCCTGA ATGTAAAACA TTATAAGATC 
CGCAAACTGG ACAGCGGTGG ATTTTACATC ACGTCCCGCA CACAGTTCAG CACATTACAG 
CAGCTGGTCA ACCACTACCG CCAGCACGCA GACGGCCTGT GCCACTCCCT GACTGACGTG 
TGTCCAGTGC TGAAGCCTCC GACTCAGGGT CTGGCACGTG ATGCCTGGGA GATTCCTCGA 
GACTCGCTGA GACTGGATGT CAAGCTGGGC CAGGGCTGCT TCGGGGAGGT CTGGATGGGC 
ACCTGGAACG GGACGACACG TGTGGCCATC AAGACTCTGA AGCCTGGCAC CATGTCTCCC 
GAAGCGTTCC TGCAGGAGGC GCAAGTGATG AAGAAGCTCC GGCATGAGAA GCTGGTGCAG 
CTTTACGCCG TCGTCTCAGA GGAGCCCATT TACATAGTCA CTGAGTACAT GGGTCAAGGA 
AGCCTGCTGG ATTTCCTGAA AGGTGACATG GGGAAAATGC TGCGTTTGCC TCAGCTCGTG 
GACATGGCCT CTCAGATCGC CTCAGGAATG GCATACGTGG AGAGAATGAA CTATGTGCAC 
AGGGACCTCA GGGCTGCCAA CATCCTGGTG GGCGATAACC TGGTGTGTAA AGTGGCTGAT 
TTCGGCCTCG CTCGACTCAT TGAGGACAAC GAATACACCG CGAGACAAGG AGCAAAGTTT 
CCTATTAAGT GGACTGCACC GGAGGCAGCT CTGTACGGAC GCTTCACCAT CAAATCTGAC 
GTGTGGTCGT TTGGGATTTT ACTGACGGAG CTCACCACCA AAGGCAGGGT GCCTTATCCA 
GGTATGGTGA ACCGCGAGGT GCTTGACCAG GTGGAGCGTG GCTACAGGAT GCCCTGCCCG 
GCCGAATGCC CTGACTCCCT GCACGAGCTC ATGCTGACCT GCTGGCGCAA AGAGCCCGAA 
GAGCGGCCCA CATTCGAGTA CCTGCAGGGT TTCCTGGAGG ACTACTTCAC CTCCACCGAA 
CCACAATACC AACCCGGAGA AAACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

src ( XM_017353519.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209008.1 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57340
Clone ID Related Accession (Same CDS sequence)	XM_017353519.1 , XM_017353519.2
Accession Version	XM_017353519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG ATCATCTCAG ATTGACTCTG 
GAGAAAAGAT GGTACTTCGG AAAAATCACA CGCAGAGACT CTGAGCGACT GCTCTTGAAT 
CTGGAGAACA GACGAGGAAC TTTCCTGGTC AGAGAGAGCG AGACCACCAA AGGTGCCTAC 
TGTCTCTCCG TCCTGGATTA CGACAACGTG AAGGGCCTGA ATGTAAAACA TTATAAGATC 
CGCAAACTGG ACAGCGGTGG ATTTTACATC ACGTCCCGCA CACAGTTCAG CACATTACAG 
CAGCTGGTCA ACCACTACCG CCAGCACGCA GACGGCCTGT GCCACTCCCT GACTGACGTG 
TGTCCAGTGC TGAAGCCTCC GACTCAGGGT CTGGCACGTG ATGCCTGGGA GATTCCTCGA 
GACTCGCTGA GACTGGATGT CAAGCTGGGC CAGGGCTGCT TCGGGGAGGT CTGGATGGGC 
ACCTGGAACG GGACGACACG TGTGGCCATC AAGACTCTGA AGCCTGGCAC CATGTCTCCC 
GAAGCGTTCC TGCAGGAGGC GCAAGTGATG AAGAAGCTCC GGCATGAGAA GCTGGTGCAG 
CTTTACGCCG TCGTCTCAGA GGAGCCCATT TACATAGTCA CTGAGTACAT GGGTCAAGGA 
AGCCTGCTGG ATTTCCTGAA AGGTGACATG GGGAAAATGC TGCGTTTGCC TCAGCTCGTG 
GACATGGCCT CTCAGATCGC CTCAGGAATG GCATACGTGG AGAGAATGAA CTATGTGCAC 
AGGGACCTCA GGGCTGCCAA CATCCTGGTG GGCGATAACC TGGTGTGTAA AGTGGCTGAT 
TTCGGCCTCG CTCGACTCAT TGAGGACAAC GAATACACCG CGAGACAAGG AGCAAAGTTT 
CCTATTAAGT GGACTGCACC GGAGGCAGCT CTGTACGGAC GCTTCACCAT CAAATCTGAC 
GTGTGGTCGT TTGGGATTTT ACTGACGGAG CTCACCACCA AAGGCAGGGT GCCTTATCCA 
GGTATGGTGA ACCGCGAGGT GCTTGACCAG GTGGAGCGTG GCTACAGGAT GCCCTGCCCG 
GCCGAATGCC CTGACTCCCT GCACGAGCTC ATGCTGACCT GCTGGCGCAA AGAGCCCGAA 
GAGCGGCCCA CATTCGAGTA CCTGCAGGGT TTCCTGGAGG ACTACTTCAC CTCCACCGAA 
CCACAATACC AACCCGGAGA AAACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209008.1
CDS	437..2086
Translation	MGGVKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTRKLNPREGDWWLARSLTTGESGYIPSNYVAPSDSIQAEDHLRLTLEKRWYFGKITRRDSERLLLNLENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	XM_017353519.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353519.2

ORF Insert Sequence:

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGAGAAA GTTGAACCCC 
AGGGAGGGTG ACTGGTGGCT AGCGCGATCA CTGACGACAG GAGAGAGCGG ATACATTCCC 
AGCAATTACG TGGCACCGTC TGACTCCATC CAGGCTGAAG ATCATCTCAG ATTGACTCTG 
GAGAAAAGAT GGTACTTCGG AAAAATCACA CGCAGAGACT CTGAGCGACT GCTCTTGAAT 
CTGGAGAACA GACGAGGAAC TTTCCTGGTC AGAGAGAGCG AGACCACCAA AGGTGCCTAC 
TGTCTCTCCG TCCTGGATTA CGACAACGTG AAGGGCCTGA ATGTAAAACA TTATAAGATC 
CGCAAACTGG ACAGCGGTGG ATTTTACATC ACGTCCCGCA CACAGTTCAG CACATTACAG 
CAGCTGGTCA ACCACTACCG CCAGCACGCA GACGGCCTGT GCCACTCCCT GACTGACGTG 
TGTCCAGTGC TGAAGCCTCC GACTCAGGGT CTGGCACGTG ATGCCTGGGA GATTCCTCGA 
GACTCGCTGA GACTGGATGT CAAGCTGGGC CAGGGCTGCT TCGGGGAGGT CTGGATGGGC 
ACCTGGAACG GGACGACACG TGTGGCCATC AAGACTCTGA AGCCTGGCAC CATGTCTCCC 
GAAGCGTTCC TGCAGGAGGC GCAAGTGATG AAGAAGCTCC GGCATGAGAA GCTGGTGCAG 
CTTTACGCCG TCGTCTCAGA GGAGCCCATT TACATAGTCA CTGAGTACAT GGGTCAAGGA 
AGCCTGCTGG ATTTCCTGAA AGGTGACATG GGGAAAATGC TGCGTTTGCC TCAGCTCGTG 
GACATGGCCT CTCAGATCGC CTCAGGAATG GCATACGTGG AGAGAATGAA CTATGTGCAC 
AGGGACCTCA GGGCTGCCAA CATCCTGGTG GGCGATAACC TGGTGTGTAA AGTGGCTGAT 
TTCGGCCTCG CTCGACTCAT TGAGGACAAC GAATACACCG CGAGACAAGG AGCAAAGTTT 
CCTATTAAGT GGACTGCACC GGAGGCAGCT CTGTACGGAC GCTTCACCAT CAAATCTGAC 
GTGTGGTCGT TTGGGATTTT ACTGACGGAG CTCACCACCA AAGGCAGGGT GCCTTATCCA 
GGTATGGTGA ACCGCGAGGT GCTTGACCAG GTGGAGCGTG GCTACAGGAT GCCCTGCCCG 
GCCGAATGCC CTGACTCCCT GCACGAGCTC ATGCTGACCT GCTGGCGCAA AGAGCCCGAA 
GAGCGGCCCA CATTCGAGTA CCTGCAGGGT TTCCTGGAGG ACTACTTCAC CTCCACCGAA 
CCACAATACC AACCCGGAGA AAACCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

src ( XM_017353520.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209009.1 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa57341
Clone ID Related Accession (Same CDS sequence)	XM_017353520.1 , XM_017353520.2
Accession Version	XM_017353520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 
CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 
TCTGACTCCA TCCAGGCTGA AGATCATCTC AGATTGACTC TGGAGAAAAG ATGGTACTTC 
GGAAAAATCA CACGCAGAGA CTCTGAGCGA CTGCTCTTGA ATCTGGAGAA CAGACGAGGA 
ACTTTCCTGG TCAGAGAGAG CGAGACCACC AAAGGTGCCT ACTGTCTCTC CGTCCTGGAT 
TACGACAACG TGAAGGGCCT GAATGTAAAA CATTATAAGA TCCGCAAACT GGACAGCGGT 
GGATTTTACA TCACGTCCCG CACACAGTTC AGCACATTAC AGCAGCTGGT CAACCACTAC 
CGCCAGCACG CAGACGGCCT GTGCCACTCC CTGACTGACG TGTGTCCAGT GCTGAAGCCT 
CCGACTCAGG GTCTGGCACG TGATGCCTGG GAGATTCCTC GAGACTCGCT GAGACTGGAT 
GTCAAGCTGG GCCAGGGCTG CTTCGGGGAG GTCTGGATGG GCACCTGGAA CGGGACGACA 
CGTGTGGCCA TCAAGACTCT GAAGCCTGGC ACCATGTCTC CCGAAGCGTT CCTGCAGGAG 
GCGCAAGTGA TGAAGAAGCT CCGGCATGAG AAGCTGGTGC AGCTTTACGC CGTCGTCTCA 
GAGGAGCCCA TTTACATAGT CACTGAGTAC ATGGGTCAAG GAAGCCTGCT GGATTTCCTG 
AAAGGTGACA TGGGGAAAAT GCTGCGTTTG CCTCAGCTCG TGGACATGGC CTCTCAGATC 
GCCTCAGGAA TGGCATACGT GGAGAGAATG AACTATGTGC ACAGGGACCT CAGGGCTGCC 
AACATCCTGG TGGGCGATAA CCTGGTGTGT AAAGTGGCTG ATTTCGGCCT CGCTCGACTC 
ATTGAGGACA ACGAATACAC CGCGAGACAA GGAGCAAAGT TTCCTATTAA GTGGACTGCA 
CCGGAGGCAG CTCTGTACGG ACGCTTCACC ATCAAATCTG ACGTGTGGTC GTTTGGGATT 
TTACTGACGG AGCTCACCAC CAAAGGCAGG GTGCCTTATC CAGGTATGGT GAACCGCGAG 
GTGCTTGACC AGGTGGAGCG TGGCTACAGG ATGCCCTGCC CGGCCGAATG CCCTGACTCC 
CTGCACGAGC TCATGCTGAC CTGCTGGCGC AAAGAGCCCG AAGAGCGGCC CACATTCGAG 
TACCTGCAGG GTTTCCTGGA GGACTACTTC ACCTCCACCG AACCACAATA CCAACCCGGA 
GAAAACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209009.1
CDS	438..2069
Translation	MGGVKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTEGDWWLARSLTTGESGYIPSNYVAPSDSIQAEDHLRLTLEKRWYFGKITRRDSERLLLNLENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	XM_017353520.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353520.1

ORF Insert Sequence:

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 
CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 
TCTGACTCCA TCCAGGCTGA AGATCATCTC AGATTGACTC TGGAGAAAAG ATGGTACTTC 
GGAAAAATCA CACGCAGAGA CTCTGAGCGA CTGCTCTTGA ATCTGGAGAA CAGACGAGGA 
ACTTTCCTGG TCAGAGAGAG CGAGACCACC AAAGGTGCCT ACTGTCTCTC CGTCCTGGAT 
TACGACAACG TGAAGGGCCT GAATGTAAAA CATTATAAGA TCCGCAAACT GGACAGCGGT 
GGATTTTACA TCACGTCCCG CACACAGTTC AGCACATTAC AGCAGCTGGT CAACCACTAC 
CGCCAGCACG CAGACGGCCT GTGCCACTCC CTGACTGACG TGTGTCCAGT GCTGAAGCCT 
CCGACTCAGG GTCTGGCACG TGATGCCTGG GAGATTCCTC GAGACTCGCT GAGACTGGAT 
GTCAAGCTGG GCCAGGGCTG CTTCGGGGAG GTCTGGATGG GCACCTGGAA CGGGACGACA 
CGTGTGGCCA TCAAGACTCT GAAGCCTGGC ACCATGTCTC CCGAAGCGTT CCTGCAGGAG 
GCGCAAGTGA TGAAGAAGCT CCGGCATGAG AAGCTGGTGC AGCTTTACGC CGTCGTCTCA 
GAGGAGCCCA TTTACATAGT CACTGAGTAC ATGGGTCAAG GAAGCCTGCT GGATTTCCTG 
AAAGGTGACA TGGGGAAAAT GCTGCGTTTG CCTCAGCTCG TGGACATGGC CTCTCAGATC 
GCCTCAGGAA TGGCATACGT GGAGAGAATG AACTATGTGC ACAGGGACCT CAGGGCTGCC 
AACATCCTGG TGGGCGATAA CCTGGTGTGT AAAGTGGCTG ATTTCGGCCT CGCTCGACTC 
ATTGAGGACA ACGAATACAC CGCGAGACAA GGAGCAAAGT TTCCTATTAA GTGGACTGCA 
CCGGAGGCAG CTCTGTACGG ACGCTTCACC ATCAAATCTG ACGTGTGGTC GTTTGGGATT 
TTACTGACGG AGCTCACCAC CAAAGGCAGG GTGCCTTATC CAGGTATGGT GAACCGCGAG 
GTGCTTGACC AGGTGGAGCG TGGCTACAGG ATGCCCTGCC CGGCCGAATG CCCTGACTCC 
CTGCACGAGC TCATGCTGAC CTGCTGGCGC AAAGAGCCCG AAGAGCGGCC CACATTCGAG 
TACCTGCAGG GTTTCCTGGA GGACTACTTC ACCTCCACCG AACCACAATA CCAACCCGGA 
GAAAACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

src ( XM_017353520.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209009.1 Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X2, mRNA.

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CloneID	ODa57341
Clone ID Related Accession (Same CDS sequence)	XM_017353520.1 , XM_017353520.2
Accession Version	XM_017353520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	proto-oncogene tyrosine-protein kinase Src isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 
CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 
TCTGACTCCA TCCAGGCTGA AGATCATCTC AGATTGACTC TGGAGAAAAG ATGGTACTTC 
GGAAAAATCA CACGCAGAGA CTCTGAGCGA CTGCTCTTGA ATCTGGAGAA CAGACGAGGA 
ACTTTCCTGG TCAGAGAGAG CGAGACCACC AAAGGTGCCT ACTGTCTCTC CGTCCTGGAT 
TACGACAACG TGAAGGGCCT GAATGTAAAA CATTATAAGA TCCGCAAACT GGACAGCGGT 
GGATTTTACA TCACGTCCCG CACACAGTTC AGCACATTAC AGCAGCTGGT CAACCACTAC 
CGCCAGCACG CAGACGGCCT GTGCCACTCC CTGACTGACG TGTGTCCAGT GCTGAAGCCT 
CCGACTCAGG GTCTGGCACG TGATGCCTGG GAGATTCCTC GAGACTCGCT GAGACTGGAT 
GTCAAGCTGG GCCAGGGCTG CTTCGGGGAG GTCTGGATGG GCACCTGGAA CGGGACGACA 
CGTGTGGCCA TCAAGACTCT GAAGCCTGGC ACCATGTCTC CCGAAGCGTT CCTGCAGGAG 
GCGCAAGTGA TGAAGAAGCT CCGGCATGAG AAGCTGGTGC AGCTTTACGC CGTCGTCTCA 
GAGGAGCCCA TTTACATAGT CACTGAGTAC ATGGGTCAAG GAAGCCTGCT GGATTTCCTG 
AAAGGTGACA TGGGGAAAAT GCTGCGTTTG CCTCAGCTCG TGGACATGGC CTCTCAGATC 
GCCTCAGGAA TGGCATACGT GGAGAGAATG AACTATGTGC ACAGGGACCT CAGGGCTGCC 
AACATCCTGG TGGGCGATAA CCTGGTGTGT AAAGTGGCTG ATTTCGGCCT CGCTCGACTC 
ATTGAGGACA ACGAATACAC CGCGAGACAA GGAGCAAAGT TTCCTATTAA GTGGACTGCA 
CCGGAGGCAG CTCTGTACGG ACGCTTCACC ATCAAATCTG ACGTGTGGTC GTTTGGGATT 
TTACTGACGG AGCTCACCAC CAAAGGCAGG GTGCCTTATC CAGGTATGGT GAACCGCGAG 
GTGCTTGACC AGGTGGAGCG TGGCTACAGG ATGCCCTGCC CGGCCGAATG CCCTGACTCC 
CTGCACGAGC TCATGCTGAC CTGCTGGCGC AAAGAGCCCG AAGAGCGGCC CACATTCGAG 
TACCTGCAGG GTTTCCTGGA GGACTACTTC ACCTCCACCG AACCACAATA CCAACCCGGA 
GAAAACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209009.1
CDS	437..2068
Translation	MGGVKSKPKELGQRSRSLDDGTGGHHHHTPNPTSFTPNRSPPVEGSRRGTQPNIINAEQALFGGVNSTTNSITSPNRIGILGGVTTFVALYDYESRTASDLSFRKGERLQIVNNTEGDWWLARSLTTGESGYIPSNYVAPSDSIQAEDHLRLTLEKRWYFGKITRRDSERLLLNLENRRGTFLVRESETTKGAYCLSVLDYDNVKGLNVKHYKIRKLDSGGFYITSRTQFSTLQQLVNHYRQHADGLCHSLTDVCPVLKPPTQGLARDAWEIPRDSLRLDVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMGQGSLLDFLKGDMGKMLRLPQLVDMASQIASGMAYVERMNYVHRDLRAANILVGDNLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPAECPDSLHELMLTCWRKEPEERPTFEYLQGFLEDYFTSTEPQYQPGENL

Target ORF information:

RefSeq Version	XM_017353520.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (src), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353520.2

ORF Insert Sequence:

ATGGGTGGAG TCAAGAGTAA ACCCAAAGAG CTGGGCCAGC GCTCGCGGAG TCTGGACGAC 
GGCACCGGAG GACACCACCA CCACACCCCC AACCCCACCT CCTTCACCCC TAACCGCAGT 
CCACCGGTGG AGGGGTCCCG GCGCGGCACA CAGCCCAACA TCATAAACGC GGAGCAGGCG 
CTCTTTGGGG GAGTGAACTC CACCACCAAC AGCATCACAT CTCCTAATCG AATCGGCATA 
TTAGGCGGCG TGACCACATT TGTGGCTCTG TATGACTACG AGTCACGAAC CGCCTCCGAT 
TTGTCATTCA GGAAAGGAGA ACGACTCCAG ATTGTCAATA ACACGGAGGG TGACTGGTGG 
CTAGCGCGAT CACTGACGAC AGGAGAGAGC GGATACATTC CCAGCAATTA CGTGGCACCG 
TCTGACTCCA TCCAGGCTGA AGATCATCTC AGATTGACTC TGGAGAAAAG ATGGTACTTC 
GGAAAAATCA CACGCAGAGA CTCTGAGCGA CTGCTCTTGA ATCTGGAGAA CAGACGAGGA 
ACTTTCCTGG TCAGAGAGAG CGAGACCACC AAAGGTGCCT ACTGTCTCTC CGTCCTGGAT 
TACGACAACG TGAAGGGCCT GAATGTAAAA CATTATAAGA TCCGCAAACT GGACAGCGGT 
GGATTTTACA TCACGTCCCG CACACAGTTC AGCACATTAC AGCAGCTGGT CAACCACTAC 
CGCCAGCACG CAGACGGCCT GTGCCACTCC CTGACTGACG TGTGTCCAGT GCTGAAGCCT 
CCGACTCAGG GTCTGGCACG TGATGCCTGG GAGATTCCTC GAGACTCGCT GAGACTGGAT 
GTCAAGCTGG GCCAGGGCTG CTTCGGGGAG GTCTGGATGG GCACCTGGAA CGGGACGACA 
CGTGTGGCCA TCAAGACTCT GAAGCCTGGC ACCATGTCTC CCGAAGCGTT CCTGCAGGAG 
GCGCAAGTGA TGAAGAAGCT CCGGCATGAG AAGCTGGTGC AGCTTTACGC CGTCGTCTCA 
GAGGAGCCCA TTTACATAGT CACTGAGTAC ATGGGTCAAG GAAGCCTGCT GGATTTCCTG 
AAAGGTGACA TGGGGAAAAT GCTGCGTTTG CCTCAGCTCG TGGACATGGC CTCTCAGATC 
GCCTCAGGAA TGGCATACGT GGAGAGAATG AACTATGTGC ACAGGGACCT CAGGGCTGCC 
AACATCCTGG TGGGCGATAA CCTGGTGTGT AAAGTGGCTG ATTTCGGCCT CGCTCGACTC 
ATTGAGGACA ACGAATACAC CGCGAGACAA GGAGCAAAGT TTCCTATTAA GTGGACTGCA 
CCGGAGGCAG CTCTGTACGG ACGCTTCACC ATCAAATCTG ACGTGTGGTC GTTTGGGATT 
TTACTGACGG AGCTCACCAC CAAAGGCAGG GTGCCTTATC CAGGTATGGT GAACCGCGAG 
GTGCTTGACC AGGTGGAGCG TGGCTACAGG ATGCCCTGCC CGGCCGAATG CCCTGACTCC 
CTGCACGAGC TCATGCTGAC CTGCTGGCGC AAAGAGCCCG AAGAGCGGCC CACATTCGAG 
TACCTGCAGG GTTTCCTGGA GGACTACTTC ACCTCCACCG AACCACAATA CCAACCCGGA 
GAAAACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A dominant gain-of-function mutation in universal tyrosine kinase SRC causes thrombocytopenia, myelofibrosis, bleeding, and bone pathologies.
Science translational medicine8(328)328ra30(2016 Mar)
Turro E,Greene D,Wijgaerts A,Thys C,Lentaigne C,Bariana TK,Westbury SK,Kelly AM,Selleslag D,Stephens JC,Papadia S,Simeoni I,Penkett CJ,Ashford S,Attwood A,Austin S,Bakchoul T,Collins P,Deevi SV,Favier R,Kostadima M,Lambert MP,Mathias M,Millar CM,Peerlinck K,Perry DJ,Schulman S,Whitehorn D,Wittevrongel C,,De Maeyer M,Rendon A,Gomez K,Erber WN,Mumford AD,Nurden P,Stirrups K,Bradley JR,Raymond FL,Laffan MA,Van Geet C,Richardson S,Freson K,Ouwehand WH

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src cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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