npas4l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npas4l
Entrez Gene ID	108449885
Full Name	neuronal PAS domain protein 4 like
Synonyms	clo
General protein information	Preferred Names neuronal PAS domain protein 4 like Names neuronal PAS domain-containing protein 4-like cloche protein cloche
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a PAS-domain-containing basic helix-loop-helix transcription factor. This gene is expressed during late gastrulation and functions as an essential transcriptional regulator of mesoderm diversification and differentiation. The encoded protein is involved in directing the specification of endothelial and haematopoietic cell lineages. Mutations in this genes result in a loss of endothelial and haematopoietic cells and an increase in cardiomyocyte numbers. This gene is located in the telomeric region of chromosome 13. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329912.1	NP_001316841.1	neuronal PAS domain-containing protein 4-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification.
Reischauer S, Stone OA, Villasenor A, Chi N, Jin SW, Martin M, Lee MT, Fukuda N, Marass M, Witty A, Fiddes I, Kuo T, Chung WS, Salek S, Lerrigo R, Alsi? J, Luo S, Tworus D, Augustine SM, Mucenieks S, Nystedt B, Giraldez AJ, Schroth GP, Andersson O, Stainier DY
Nature535(7611)294-8(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identifying master regulator of endothelial and haematopoietic fate, cloche, enhances our understanding of early mesoderm diversification and may lead to improved protocols for the generation of endothelial and haematopoietic cells in vivo and in vitro
Title: Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification.

the disorganized retinal phenotype of clo-/- embryos is consistent with a neural and glial developmental patterning role for the early ocular vasculature that is independent of its eventual function in gas exchange
Title: Abnormal retinal development in Cloche mutant zebrafish.

The endothelial cells in clo(172) mutant embryos, although initially developed, failed to form the functional vascular system eventually.[Cloche]
Title: Characterization of a weak allele of zebrafish cloche mutant.

cloche(172) gene was mapped on the telomere of zebrafish 13 chromosome. Numerous red blood cells were observed in the cloche(172) mutant.
Title: [Positional cloning of a novel allele of zebrafish cloche mutant].

The following npas4l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npas4l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98492	NM_001329912.1 Latest version!	Danio rerio neuronal PAS domain protein 4 like (npas4l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npas4l ( NM_001329912.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001316841.1 Danio rerio neuronal PAS domain protein 4 like (npas4l), mRNA.

Price & Availability↑

CloneID	ODa98492
Clone ID Related Accession (Same CDS sequence)	NM_001329912.1
Accession Version	NM_001329912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	neuronal PAS domain-containing protein 4-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KX066019.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KX066019.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCTGCG GAGACTCGGG CATCAGGAGG ATCCTCCGCG CGTCCAAGCG CTTCAGGTCC 
ACCAAAGGAG CATCTAAGGC GCGGCGTGAT CAGATGAACA GTGAGATACG GAACCTGCGA 
GCGCTGCTGC CCATCAGCCC AGAGCACAGA CTCTCATACC TGCACAGCAT GAGCATCACC 
TGCACCTACA TCAGGAAGAG TGTGGAGCTG AGAGGTGTGT GTGAGGAATC CACAGTTTTT 
TCTGCCGTGA ATGGATGTGT CCCGCAGGAC TGTGCTCTAC AGGACTGTGC TCTACAGGAG 
TGTGTTCTGC AGGAGTGTGT TCTGCAGGAG TGTGTTCTGC AGGAGTGTGT TCTGCAGGCT 
CTGCCGGGGT TTATCGTTGC CTTTACCACA GATGGAAAAC TGCTGTATGT GTCTGAGAAC 
GTACACGAGT ATCTGGGTCT GTCCATGGTG GACGTTCTCC AGAGCGACTC TTTCTTCGAT 
ATGCTGGACC GCAGTGATGT GGAGGCGGTG AGGAGTGTGT TAGCTGACGC CAGTCCTTCA 
GGAGAGCGGT GGGTTGTGTG TCGTATGCTG GTGTCTAAAG CGATGCGTCT GCGGAGCTCC 
TGCTGCCCCC TGCTGGTGCG GATCCGTCTG CGGGACGGAG TGTGTGTGTC GCTCTGCAGA 
CCCACCGCTG ACCGGCTGCC GGCGCGCAAC GCTGACTTCC ACACACACCA CAGCGCAGAC 
ATGAGGCTCG CCAGCGCATC CTCCAGTGTG TTGTTCCACC TGGGCTTCTC AGCGGACGAG 
CTGATTGGTC GATCCTGGTA CGAGCTCCTG CACCCCGACG ACCTCCGACA CGCTGCTGAC 
CGCCACGCCG CAATCCTTGC AGCCGCCACA GCAGACGCAG AGATGCTGAT CCGGGTTCAG 
TGTAAGGATC TGTCCTGGGT GTGGATGTAC ACACACGCAT CAGCAACAGC AGAGCGGGAC 
GCGATCAGCT GCTCCAACTA CATGATCAGT GAAGCAGAGG CAGTGTATCT GCAGCAGAGA 
CTCTCCTCCT CCTCCTCCTC CTCCACCTCC TCCTCCTCCT CCTCTGCCTC TCCTCAGTGT 
AGCTCCGTCT CGGACAGCAG TGATGCCCAC AGCGCACACA CACTCTTCTG CACGCCGCCC 
TACAGCCCCA CTTCCTCTCA GTGCTCTGAC TTCCTGTCTG AAGGTTATGG CTCTCTGGAG 
GCGCTGGTGG ATTCTGCCTT CTGCTCTCCG CCGTACCCGC CGCTGTTCCC CAACCCATGC 
TGCACCCCCA CTGTCTGCCC ACCGGACACT GCCCTGGACC CCGCTCGGCT CTGCCCGCCA 
GATTCTGCCC TGGTCCCCGC TGCGCTCTGT CCGCCGGACG CTGCCCTGGA CCCCACTCAG 
CTCTGCCCGC CGGGAGCTGC CCTGGACCCC ACTCTGCTCT GCCCGCCGGG AGCTGCCCTG 
GACCCCACTC AGCTCTGCCT GCCGGACGGT GCCTTGGTCC CTGCTGTGTT CTGCCCGCCG 
AACGGTGCCC TGGTCCCTGC CCGGCTCTGC CCGTCAGATG CTGCCCTGGT CCCCGCTGCA 
CTCGGTCGGC CGGACGCTGC CCTGGTGCCT GTGTGTCGGC CACTGCAGGT GTGTGAGTGC 
CCTCTGGATG GCGCTGTGCC GCTGGAGGAC CTCAGCATGT TCCCGCTGCC GCAGGGCGGA 
GGCTCTCGTC TGATGCCGCC TGAAGCATCA CCCACTACCC ACACACACTT CTCGTATGAT 
GCAGCACAGC AGGCGGGGAT CGGCACGTTG GCAATGCAGA TACACACACT CATCTGCAGC 
TTCGATGCGT ACAGCGCCGC ACGACACACA CACTCTCACC ACACCTCCTG CTGGGCACCA 
GAGCCACTGC TGGACGAGGG CATCATCGAC AGCATCCTCA GAGAGCTGGA CAGCACACAC 
ACTGATGCAC ACACACTGAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316841.1
CDS	225..2168
Translation	MSCGDSGIRRILRASKRFRSTKGASKARRDQMNSEIRNLRALLPISPEHRLSYLHSMSITCTYIRKSVELRGVCEESTVFSAVNGCVPQDCALQDCALQECVLQECVLQECVLQECVLQALPGFIVAFTTDGKLLYVSENVHEYLGLSMVDVLQSDSFFDMLDRSDVEAVRSVLADASPSGERWVVCRMLVSKAMRLRSSCCPLLVRIRLRDGVCVSLCRPTADRLPARNADFHTHHSADMRLASASSSVLFHLGFSADELIGRSWYELLHPDDLRHAADRHAAILAAATADAEMLIRVQCKDLSWVWMYTHASATAERDAISCSNYMISEAEAVYLQQRLSSSSSSSTSSSSSSASPQCSSVSDSSDAHSAHTLFCTPPYSPTSSQCSDFLSEGYGSLEALVDSAFCSPPYPPLFPNPCCTPTVCPPDTALDPARLCPPDSALVPAALCPPDAALDPTQLCPPGAALDPTLLCPPGAALDPTQLCLPDGALVPAVFCPPNGALVPARLCPSDAALVPAALGRPDAALVPVCRPLQVCECPLDGAVPLEDLSMFPLPQGGGSRLMPPEASPTTHTHFSYDAAQQAGIGTLAMQIHTLICSFDAYSAARHTHSHHTSCWAPEPLLDEGIIDSILRELDSTHTDAHTLI

Target ORF information:

RefSeq Version	NM_001329912.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuronal PAS domain protein 4 like (npas4l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329912.1

ORF Insert Sequence:

ATGAGCTGCG GAGACTCGGG CATCAGGAGG ATCCTCCGCG CGTCCAAGCG CTTCAGGTCC 
ACCAAAGGAG CATCTAAGGC GCGGCGTGAT CAGATGAACA GTGAGATACG GAACCTGCGA 
GCGCTGCTGC CCATCAGCCC AGAGCACAGA CTCTCATACC TGCACAGCAT GAGCATCACC 
TGCACCTACA TCAGGAAGAG TGTGGAGCTG AGAGGTGTGT GTGAGGAATC CACAGTTTTT 
TCTGCCGTGA ATGGATGTGT CCCGCAGGAC TGTGCTCTAC AGGACTGTGC TCTACAGGAG 
TGTGTTCTGC AGGAGTGTGT TCTGCAGGAG TGTGTTCTGC AGGAGTGTGT TCTGCAGGCT 
CTGCCGGGGT TTATCGTTGC CTTTACCACA GATGGAAAAC TGCTGTATGT GTCTGAGAAC 
GTACACGAGT ATCTGGGTCT GTCCATGGTG GACGTTCTCC AGAGCGACTC TTTCTTCGAT 
ATGCTGGACC GCAGTGATGT GGAGGCGGTG AGGAGTGTGT TAGCTGACGC CAGTCCTTCA 
GGAGAGCGGT GGGTTGTGTG TCGTATGCTG GTGTCTAAAG CGATGCGTCT GCGGAGCTCC 
TGCTGCCCCC TGCTGGTGCG GATCCGTCTG CGGGACGGAG TGTGTGTGTC GCTCTGCAGA 
CCCACCGCTG ACCGGCTGCC GGCGCGCAAC GCTGACTTCC ACACACACCA CAGCGCAGAC 
ATGAGGCTCG CCAGCGCATC CTCCAGTGTG TTGTTCCACC TGGGCTTCTC AGCGGACGAG 
CTGATTGGTC GATCCTGGTA CGAGCTCCTG CACCCCGACG ACCTCCGACA CGCTGCTGAC 
CGCCACGCCG CAATCCTTGC AGCCGCCACA GCAGACGCAG AGATGCTGAT CCGGGTTCAG 
TGTAAGGATC TGTCCTGGGT GTGGATGTAC ACACACGCAT CAGCAACAGC AGAGCGGGAC 
GCGATCAGCT GCTCCAACTA CATGATCAGT GAAGCAGAGG CAGTGTATCT GCAGCAGAGA 
CTCTCCTCCT CCTCCTCCTC CTCCACCTCC TCCTCCTCCT CCTCTGCCTC TCCTCAGTGT 
AGCTCCGTCT CGGACAGCAG TGATGCCCAC AGCGCACACA CACTCTTCTG CACGCCGCCC 
TACAGCCCCA CTTCCTCTCA GTGCTCTGAC TTCCTGTCTG AAGGTTATGG CTCTCTGGAG 
GCGCTGGTGG ATTCTGCCTT CTGCTCTCCG CCGTACCCGC CGCTGTTCCC CAACCCATGC 
TGCACCCCCA CTGTCTGCCC ACCGGACACT GCCCTGGACC CCGCTCGGCT CTGCCCGCCA 
GATTCTGCCC TGGTCCCCGC TGCGCTCTGT CCGCCGGACG CTGCCCTGGA CCCCACTCAG 
CTCTGCCCGC CGGGAGCTGC CCTGGACCCC ACTCTGCTCT GCCCGCCGGG AGCTGCCCTG 
GACCCCACTC AGCTCTGCCT GCCGGACGGT GCCTTGGTCC CTGCTGTGTT CTGCCCGCCG 
AACGGTGCCC TGGTCCCTGC CCGGCTCTGC CCGTCAGATG CTGCCCTGGT CCCCGCTGCA 
CTCGGTCGGC CGGACGCTGC CCTGGTGCCT GTGTGTCGGC CACTGCAGGT GTGTGAGTGC 
CCTCTGGATG GCGCTGTGCC GCTGGAGGAC CTCAGCATGT TCCCGCTGCC GCAGGGCGGA 
GGCTCTCGTC TGATGCCGCC TGAAGCATCA CCCACTACCC ACACACACTT CTCGTATGAT 
GCAGCACAGC AGGCGGGGAT CGGCACGTTG GCAATGCAGA TACACACACT CATCTGCAGC 
TTCGATGCGT ACAGCGCCGC ACGACACACA CACTCTCACC ACACCTCCTG CTGGGCACCA 
GAGCCACTGC TGGACGAGGG CATCATCGAC AGCATCCTCA GAGAGCTGGA CAGCACACAC 
ACTGATGCAC ACACACTGAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification.
Nature535(7611)294-8(2016 07)
Reischauer S,Stone OA,Villasenor A,Chi N,Jin SW,Martin M,Lee MT,Fukuda N,Marass M,Witty A,Fiddes I,Kuo T,Chung WS,Salek S,Lerrigo R,Alsi? J,Luo S,Tworus D,Augustine SM,Mucenieks S,Nystedt B,Giraldez AJ,Schroth GP,Andersson O,Stainier DY

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npas4l cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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