OTOFB cDNA ORF clone, Danio rerio(Zebrafish)

The following OTOFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149766 XM_021467486.1
Latest version!
Danio rerio otoferlin b (otofb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa49454 XM_021467487.1
Latest version!
Danio rerio otoferlin b (otofb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa55765 XM_017351874.2
Latest version!
Danio rerio otoferlin b (otofb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
ODa55765 XM_017351874.1 Danio rerio otoferlin b (otofb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149766
    Clone ID Related Accession (Same CDS sequence) XM_021467486.1
    Accession Version XM_021467486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCGGA CATTGCTATG
    CTGTGGTTCT TGATGCCGCT AAAGGCAATC CGGAATCTCG TCTGCAACCA ATACAAGTGG
    CTGGTCATCA AATTAGTGTT GGCGCTGATG CTTCTCGCCA TTCTGGGCCT GTTCCTCTAC
    AGTATGCCTG GTTACATGGT GAAAAAGCTT CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323161.1
    CDS241..5562
    Translation

    Target ORF information:

    RefSeq Version XM_021467486.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin b (otofb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCGGA CATTGCTATG
    CTGTGGTTCT TGATGCCGCT AAAGGCAATC CGGAATCTCG TCTGCAACCA ATACAAGTGG
    CTGGTCATCA AATTAGTGTT GGCGCTGATG CTTCTCGCCA TTCTGGGCCT GTTCCTCTAC
    AGTATGCCTG GTTACATGGT GAAAAAGCTT CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49454
    Clone ID Related Accession (Same CDS sequence) XM_021467487.1
    Accession Version XM_021467487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCCGA CACTGCTATG
    CTGTGGTTCT TGATGCCGCT AAAGGCAATC CGGCACCTCG TCTGCAACCA ATACAAGTGG
    CTGGCCATCA AATTAGTGTT GGCGCTGATG CTTCTCGCCA TTCTGGGCCT GTTCCTCTAC
    AGTATGCCTG GTTACATGGT GAAAAAGCTT CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323162.1
    CDS241..5562
    Translation

    Target ORF information:

    RefSeq Version XM_021467487.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin b (otofb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCCGA CACTGCTATG
    CTGTGGTTCT TGATGCCGCT AAAGGCAATC CGGCACCTCG TCTGCAACCA ATACAAGTGG
    CTGGCCATCA AATTAGTGTT GGCGCTGATG CTTCTCGCCA TTCTGGGCCT GTTCCTCTAC
    AGTATGCCTG GTTACATGGT GAAAAAGCTT CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55765
    Clone ID Related Accession (Same CDS sequence) XM_017351874.2 , XM_017351874.1
    Accession Version XM_017351874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCTGA CACTGCATTC
    CTGTGGTTCA TGATGCCGCT AAAGGCAATC CGCCATCTCG TCTGCAACCA ATACAAGTGG
    CTGGTCATCA AAATAGTGGT GGCTCTGCTG CTTTTAGCAA TGCTGGGCTT GTTTCTGTAC
    AGTATGCCAG GGTACATGGT GAAAAAGCTA CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207363.1
    CDS240..5561
    Translation

    Target ORF information:

    RefSeq Version XM_017351874.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio otoferlin b (otofb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCTGA CACTGCATTC
    CTGTGGTTCA TGATGCCGCT AAAGGCAATC CGCCATCTCG TCTGCAACCA ATACAAGTGG
    CTGGTCATCA AAATAGTGGT GGCTCTGCTG CTTTTAGCAA TGCTGGGCTT GTTTCTGTAC
    AGTATGCCAG GGTACATGGT GAAAAAGCTA CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55765
    Clone ID Related Accession (Same CDS sequence) XM_017351874.2 , XM_017351874.1
    Accession Version XM_017351874.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product otoferlin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCTGA CACTGCATTC
    CTGTGGTTCA TGATGCCGCT AAAGGCAATC CGCCATCTCG TCTGCAACCA ATACAAGTGG
    CTGGTCATCA AAATAGTGGT GGCTCTGCTG CTTTTAGCAA TGCTGGGCTT GTTTCTGTAC
    AGTATGCCAG GGTACATGGT GAAAAAGCTA CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207363.1
    CDS193..5514
    Translation

    Target ORF information:

    RefSeq Version XM_017351874.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio otoferlin b (otofb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAAGCGTA ATAAGCATCG CTCCTCCAAG GATGATGGCA AAGGTCAAGA TGAACCAGCC 
    ATTCTGGAGA CGGAGGACAT GAGGATGCCG GACCCTGACA CCATCTCACT GGCATCCGTG
    ACTGCTGTCA CCACCAATGT CTCCAACAAG AGATCAAAGC CTGACATCAA GATTGAGCCT
    AGTGCTGGAA GACCAATGGA TTTCCAAGTC AGTGTTACTG TGATAGAGGC TCGACAGCTG
    GTGGGCTTGA ACATGGATCC AGTGGTGTGT GTAGAAATTG GTGATGATAA AAAATACACT
    TCAATGAAGG AGTCGACCAA CTGCCCTTAC TACAATGAGT ACTTCGTTTT TGACTTCCAC
    GTTCCTCCTG ATGTGATGTT CGACAAAATC TTGAAAGTGT CGGTGATCCA TTCGAAGAAT
    CTTCTGCGAA GCGGCACGCT GGTCGGGAGT TTTAAGCTTG ATGTTGGCAC TATATACACA
    CAACCTGACC ATCAGTTCTT CCACAAATGG GCGACATTAT GTGATCCCGA AGACATCACT
    GCCGGATTGA AGGGCTATGT CAAGTGTGAC ATTGCTGTCG TGGCGAAGGG CGACACAATT
    AAAACTCCAC ACAAAGCCAA TGAAAATGAT GAGGACGACA TTGAGAGTAA TCTGCTGCTG
    CCGGAGGACG TTCCAGCGGA GCGCCAGTGG GCCAGATTCT ACCTGAAAAT ATACAGAGCC
    GAGGGCTTGC CCAAAATGAA CACCAGCATC ATGGCAAATG TCAAAAAAGC TTTCATTGGA
    GAAAATAAGG ACTTGGTTGA TCCGTACGTT CAAGTGCTGT TTGCAGGACA GAAGGGGAAA
    ACTTCGATTC AGAAGAGCAG CTACGAGCCC ATCTGGAACG AGCAGATCGT ATTCACCGAG
    CAGTTTCCAC CGCTGTGTCG GAGGATGAAG CTCCAGATCC GGGATTCAGA CAAAGTGAAT
    GATGTGGCCA TCGGTACACA CTTCATTGAC CTGCGGAAGA TCGCCAATGA TGGAGACAAA
    GGCTTTCTCC CGACTCTTGG CCCGGCGTGG GTCAACATGT ACGGCTCCAC GCGCAGCTAC
    ACGCTCATGG ATGAGTATCA GGACCTGAAC GAAGGCCTCG GGGAGGGCGT CTCCTTCAGG
    GCACGTCTGC TGATCAGCCT GGCCGTGGAG ATCCTGGACA CCTCGTCTCC TGAAGTCATG
    AGCTCCACTG AGGTGCAGGT GGAGGGGATA CCCAACATCT CTGATAATGC CACAGGAAAA
    GTGGAGGAAT TCTTTCTCTT TGGAAGTTTC CTCGAGGCCA CCATGATTGA CAGGAAGATC
    GGAGACAAAC CCATCAACTT TGAGGTCACA ATAGGAAACT ATGGGAGTGA GCTCGATACT
    CCAACCAAGC CCAAATCGAA GAAGAAGAAG GGAGATGGCG ATGACGAGGA GTCTGAGCTG
    ATTCACGGCT CTAGCGACGA GGAGGTGGAT GATGGTGGAG ACGTGACGTC GGTTTCCACA
    ACTCCTCCAA TGAAGCCAGT CATCACCGAC AGGAATTATT TCCACCTGCC GTACTTTGAG
    AGGAAGCCCT GCATCTACAT CTACAGCTGG TGGCAGGACC AACGACGACG ACTTTATAAT
    GCTAACGTCT TGGACAAAAT GGCAGATAAA CTGGAAGAAG GGTTGAATGA TGTGCAGGAG
    ATCATAAAGA CAGAGAAGGC CTATCCAGAG CGCAGACTCC GAGGAGTTCT GGAGGAGCTC
    AGCAACAGCT GCAGTACCTT TGTATCTTTG GCAAACAATG ACCAGAATCA GGCTGGCAGG
    ACTAAACTGG ACAAGGAGAG AGTCAAATTG TGCATGCAGG AAGTGGAAAG CATGGGCCAG
    CAAGCGAAAA CCATGAGGTC ACAAGTGAAG AAAAGCAACG TGAAAGACAG AATAAAGCTG
    GCTCAAAACT TCCTCCACAA GCTGAGATTC TTGTCTGATG AGCCTCAACA CAGCGTTCCA
    GACATTTACA TCTGGATGAT AAGCAACAAC AAGCGCATTG CATACGCCCG CGTTCCCTCT
    AAAGACATCC TCTACTCTTC TGTGGAGGAG GAGTGTGGAA AGGACTGCGG AAAGGTCCAG
    ACCATCTTCT TCAAGCTGCC TGGCAAGAAG AGTTTTGGAC CTGCGGGCTG GACGGTTCAG
    GCAAAGACTG AGCTGTACCT GTGGCTGGGC CTGAACAGGC AGCGTAAGGA CTATCTATGC
    GGTCTGCCTA ACGGGTTCGA GGAGAACAAG GCAGTGAAGG GGCCGGGCGT TCAGTCCTCT
    CCCCCCATCA GCCTGATGTA CACCATGAAA CAGATCTTCC AGCTGAGGGT CCACATGTAC
    CAGGCTCGCA GTCTGTTTGC GGCAGACAGC ACAGGCCTCT CGGATCCTTT CGCCAGGATA
    TTTTTTTCGA CTCACAGTCA AGTCACAGAG GTACTGAACG AGACGCTGTG TCCCACATGG
    GATCAGCTGC TGGTGTTTGA TCATGTGGAG CTCTACGGTG AAGCTGGAGA ACTGAGAGAC
    GATCCTCCGA TAATCGTCAT CGAGATCTAC GACCAGGACA CAGTGGGGAA AGCTGATTTC
    ATGGGAAGGA CGTTCGCTAA ACCGATCACC AAGATGTCAG ATGAGCACTA CGGGCCTCCG
    CGTTTCCCTC CTCAGCTGGA GTACTATCAG ATCTACAGGG GAAACTGCAC GGCTGGAGAA
    ATGCTGGCCG CCTTTGAGCT GCTGCAGATT GGACCAGGTG GAAAGGCTGA CCTGCCGCCA
    ATAGACGGAC CTACAGATAT CGACCGTGGC CCAATCCTGC CGGTGCCTAT CGGCATTAGA
    CCTGTCCTGA GTAAATACAG GATTGAGGTT CTCTTTTGGG GTCTGAGGGA CCTGAAGCGG
    GTGAATCTGG CTCAAGTGGA CCGTCCCCGT GTTGACATTG AGTGCGCTGG GAAAGGCGTT
    CAGTCGTCGC TGATCCAGAA CTACAAGAAA AACCCCAACT TCAGCACTCT GGTCAAGTGG
    TTTGAAGTGG ATCTGCCTGA GAATGAGCTC CTCCATCCTC CGCTGAACAT CCGCGTGGTG
    GACTGCCGAG CGTTTGGCCG CTACACGCTG GTCGGCTCTC ATGCCGTCAC AACTCTGCGC
    AAGTTCATCT ACAGGCCTGC AGACAAGAGT GTCAATAACT GGTCTGCTAT GGAAGAGGTT
    GTGATCCACA CAGAGCCTGA ACCAGCAGTG AAGAAGATCG AGACAGTCGT CAAACTGGAC
    TCAGCATCTG ATGCTGTGGT CAAAGTTGAT ATGCCGGATG ATGAGAAGGG TGGGAAAGGT
    AAGAAGAAGC GTAAGAAGGG TGAAGAGGTG GAGGAAGAGG AACTGGATGA GAGCATGCTG
    GACTGGTGGT CCAAATACTT CGCCTCCATT GAGACTCTGA AAGAGGCCAT GAAGGCACAA
    GAAGCAGAAG CTGAAGAAAA AGAGGATTTT GAATCTGGAG ATGGGAAGAA GAAGAGAGGA
    AAGAAGAGTA AAACCATACC TGGAGAAGCC GTGCCGGAGA AGAAGAAGCA GAAGATTGAA
    GAGTTCAAGG TGTACAACCG AGAGCTGGAG TATGAATATA ACCAGTTTGA GGACTGGCTT
    CACACCTTCA ACCTTCACAG GGGGAAGTGC TCAGACGATG CGGACGTGGA TCAGAATGCA
    GCTGATGAAG ACAGACTCAT TGGCAAGTTC AAGGGCTCCT TGTGTATCTA CAAAGTCTCC
    TCAGATGATA TGTCCAGAGA CATGGGCTTT GACTCCAACA TGGGAATGTT CCAGAACATC
    CCACACAACG ATCCAATAAA CGTGCTTGTC CGAATCTACG TGGTCCGGGC GACAGATCTG
    CACCCTGCAG ACATCAATGG AAAGGCTGAT CCATACATCG CCATCAAGCT TGGAAAGTCC
    GAAATCAAGG ACAAGGAGAA TTACATCTCA AAACAGCTCA ACCCTGTGTT TGGAAAGTCG
    TTTGATGTTG AAGCAACTCT CCCAATGGAC TCCACCCTCA CTGTGTCCAT CTATGACTGG
    GATTTGGTGG GAACTGATGA CCTGATCGGC GAGACTAAGA TTGACTTGGA GAACCGTTTC
    TACAGCAAAC ACAGAGCCAC ATGTGGCATC AGCTCCACCT ACGCCATTCA TGGCTATAAT
    GTGTGGCGGG ACCCATTGAA GCCATCGCAC ATTCTTGCCA AACTCTGCAA AGATGGAAAG
    ATAGATGCGC CGCAGTATGG CCCAGGTGGA AGAGTGAAAG TGACAAACAG AGTTTACACA
    GGGCCAACTG AAGTCGAGGA TGAAAATGGA CTAAAGAAAC AGACGGATGA GCATTTAGCT
    CTTGCTGTAC TGAACCACTG GGAAGATATT CCTCGGATTG GCTGTAAACT GGTTCCAGAA
    CACATGGAGA CGAGACCTCT CCTGAATCCA GATAAACCTG GGATTGAACA GGGGAGGATT
    GAGATGTGGG TAGACATGTT CCCCAAGGAC ATGTCTGCGC CTGGACCTGC TCTAGATATC
    TCACCGAGGA AGCCAAAGAA ATTTGAGCTC AGAGTGATCG TGTGGAACAC AGACGAAGTC
    GTTCTTGAGG ACGATGACAT TTTCACTGGA GAAAAATCAA GTGACATCTT TGTTAGAGGA
    TGGCTGAAAG GCCAACAAGA GGATAAGCAG GACACAGATG TTCACTATCA CTCACTGACT
    GGAGAGGGAA ATTTCAACTG GCGCTTCATT TATCCTTTCG ACTACCTGCA GGCCGAAGAG
    AAAATTGTCA TCTCAAAGAA AGAGTCCATG TTTTCCTGGG ATGAGACTGA GTACAAAATT
    CCTGCCCGAC TGAACTTGCA AGTGTGGGAT GCAGATCATT TCTCTGCTGA CGACTTCTTG
    GGTGCGATCG AATTGGACCT GAATCGATTT CCACGAGGCG CAAAGACGGC AAAGCAGTGC
    TCCATTGAAA TGGTCACGAA TGAGAATGAA ATGCCCATGG TCAACATCTT CAAGCAGAAG
    AGAATCAAAG GCTGGTGGCC GTTTGTGGCC AGAGATGAAA ATGATGAGCT AGAAATTACA
    GGCAAAGTGG AAGCAGAGCT TCAGTTGTTG ACAGGTGAGG AGGCTGAGAA AAGTCCCGTC
    GGTGAAGGAC GCAATGAGCC AGAGCCTCTG GAGAAACCCA ACCGCCCTGA CACTGCATTC
    CTGTGGTTCA TGATGCCGCT AAAGGCAATC CGCCATCTCG TCTGCAACCA ATACAAGTGG
    CTGGTCATCA AAATAGTGGT GGCTCTGCTG CTTTTAGCAA TGCTGGGCTT GTTTCTGTAC
    AGTATGCCAG GGTACATGGT GAAAAAGCTA CTGGGAGCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.