SI:DKEYP-115E12.6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:DKEYP-115E12.6
Entrez Gene ID	100005596
Full Name	si:dkeyp-115e12.6
General protein information	Preferred Names si:dkeyp-115e12.6 Names centromere protein F-like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021472747.1	XP_021328422.1	centromere protein F-like isoform X2
XM_001344567.7	XP_001344603.3	centromere protein F-like isoform X1
XM_005157165.3	XP_005157222.1	centromere protein F-like isoform X2
XM_001344567.8	XP_001344603.4	centromere protein F-like isoform X1
XM_021481112.1	XP_021336787.1	centromere protein F-like isoform X1
XM_021481113.1	XP_021336788.1	centromere protein F-like isoform X2
XM_005157164.3	XP_005157221.1	centromere protein F-like isoform X1

Pathways from BioSystems
Gene Ontology

The following SI:DKEYP-115E12.6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEYP-115E12.6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145581	XM_001344567.8 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145582	XM_021472747.1 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa19387	XM_005157164.3 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa19387	XM_001344567.7 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa19387	XM_021481112.1 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa19388	XM_021481113.1 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa19388	XM_005157165.3 Latest version!	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:DKEYP-115E12.6 ( XM_001344567.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001344603.4 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X3, mRNA.

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CloneID	ODa145581
Clone ID Related Accession (Same CDS sequence)	XM_001344567.8
Accession Version	XM_001344567.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centromere protein F-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001344567.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTTCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG ACAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTATAGG AGTCCAAACA 
CTTCCAAGTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCGGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTG 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG GGTTTGGAAG AGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATGG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTAAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAGCTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACCAGGAC GAATGATCTC 
TCTGCACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACATAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAT TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAACA TTTTAAACAA 
CAGTCTGTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACAGTA TTCCAGAACA TTCAATTCAT GATATCAAGG AGCTCGAACG AGTTACAAAG 
GAATTACAAG CAGCTCAACT CGAGCTAAAT GTGATGAAGG CTCAGAATTC AGAGCTTGCT 
CTACAGGGAG ACATGATAAA AGATAAGCTA CTGAATGTTC AGCAAGAGAA TCAAGAGTTG 
AAATCAACTC TGAAACACTT GTGTGAAGAC ACTGGTGCTA AACAAGATGG TGCCTTAAAT 
TTAGCGAACT CTAAAAACAA ATCGTTGTTG AGTTCAAATG AAGGATTGCA AAGCATCTCC 
CCATTAGGGA AAGAGAAATT GGTCGTTCAG CATGGAAACT CTGAAGCGGA GGTTCAGTCT 
CTTCAGGAGC AGATCCAGAA GTTGCAGGAC CAAATCCTGG ACCTCTTTGA ACAAAACAGG 
ACTCAGGCTG AACAGCTGGA GCTCTGGAAG CTGTCCACTG TGGAAGAAAC AGCGGGCAAC 
AACTCACCCT CTATTGTGCT TAAAGAGTTT GAGCTGTTCC TCCCCTGCGG TCCTAGCAAA 
CTGCACACAC AGTCTCAAAC CAGGACCATG ATGCACCAGT GCGATGTCAG AGATGTGACT 
TATCACTCTG GATTAGATGA CTCAAATCTG ACCAACAGTG ACGAAAAAGT GTCGTCTAAA 
ACCCAAATAA CAATTGATGT GAGAAATTCG CAAGGACCTG CTGAGGACAT TATGGCTAGA 
AATAAGAAGC TGCTAACCAA GGATAATGCT CAACACAAGT GTGAGAACAG CTCTGCTAAC 
CACCACTGCG ATAAAAGTCC TCCTGTCTGC ATCTCAGATA TAATGACTAG TAACACTCGT 
GACAAGCTGT CACCGCTACA GACCAAACCA ACAGATTACA GTCATTCAGA GTCTCAGCCG 
GTTGCCTCAT TGTCTCCTGA GGGTCAGCCC AAAGTGACAG ATGCCAGTGC TAACAGCCAG 
GAAGATGCCT TAATCAGTGT TTCCATCTCA ACCAAATATC AGGCCGAGGT AATCAACACA 
AATGCAATCA CTGAGTCCTA TGGGAACGCT GATTTCCACA ATTCTGAAAC TATGGGAACT 
GTTGCTTTGG ATAAACATCG TGGAGATGCA ACAGATAAAC AGAACAAACA TTACACTGCT 
GTGGAAAACA CAATCAGAAA CGAATCAGCT CAAGGTCAAA AAGCAGAGGT CAGTGGAGTT 
TGTAACAGAA CAGGGGTCAT AAGTGTGTCC ACGCAGATGG AGGAGAGTCG AGAGATGCAT 
TTAGCAGTCA AACAGCCGCC CGTCCACGCC TGCACTCAGA CAGATGGAGA ACAGCTGCAG 
ACGGAGCAAG ATGAAGGTGA TAAAGCGTCT GTAGACTCAC CGGCCCTCTC CCCGACCCCA 
CACACTGTTA CAAACCAACT GCTGCTATCC AGCGTGTTCC CTATGAGTGA CCCAGCACAC 
CTCGCTGAAC GCATCCGGCA AAACCGCAAC CGCATGTCTG CTGCTTATGA TGAGACCGAA 
TATGAACCAT ACGGCCTGCC AGAGGTTGTA ATGAAAGGAT TTGCCGACAT CCCCAGTGGG 
CCAGCCTGTC CGTATGTCCT GCGCAGAGGG CTTCTGGGAA CTGACGCAAT GCCCCTCCCA 
CGCAGAGGAG AAGCCACGCC CCTGGAGGAA GATGACGACA TAGAGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001344603.4
CDS	320..5410
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAASPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIDKFESEEKSQKRIGVQTLPSGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTGLEEKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTMVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSAPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSEHTVQLPVEASVIQNIDQTNTSEISDKLQATEHFKQQSVDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDSIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQGDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQILDLFEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGPSKLHTQSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSKTQITIDVRNSQGPAEDIMARNKKLLTKDNAQHKCENSSANHHCDKSPPVCISDIMTSNTRDKLSPLQTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVCNRTGVISVSTQMEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_001344567.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001344567.8

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTTCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG ACAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTATAGG AGTCCAAACA 
CTTCCAAGTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCGGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTG 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG GGTTTGGAAG AGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATGG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTAAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAGCTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACCAGGAC GAATGATCTC 
TCTGCACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACATAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAT TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAACA TTTTAAACAA 
CAGTCTGTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACAGTA TTCCAGAACA TTCAATTCAT GATATCAAGG AGCTCGAACG AGTTACAAAG 
GAATTACAAG CAGCTCAACT CGAGCTAAAT GTGATGAAGG CTCAGAATTC AGAGCTTGCT 
CTACAGGGAG ACATGATAAA AGATAAGCTA CTGAATGTTC AGCAAGAGAA TCAAGAGTTG 
AAATCAACTC TGAAACACTT GTGTGAAGAC ACTGGTGCTA AACAAGATGG TGCCTTAAAT 
TTAGCGAACT CTAAAAACAA ATCGTTGTTG AGTTCAAATG AAGGATTGCA AAGCATCTCC 
CCATTAGGGA AAGAGAAATT GGTCGTTCAG CATGGAAACT CTGAAGCGGA GGTTCAGTCT 
CTTCAGGAGC AGATCCAGAA GTTGCAGGAC CAAATCCTGG ACCTCTTTGA ACAAAACAGG 
ACTCAGGCTG AACAGCTGGA GCTCTGGAAG CTGTCCACTG TGGAAGAAAC AGCGGGCAAC 
AACTCACCCT CTATTGTGCT TAAAGAGTTT GAGCTGTTCC TCCCCTGCGG TCCTAGCAAA 
CTGCACACAC AGTCTCAAAC CAGGACCATG ATGCACCAGT GCGATGTCAG AGATGTGACT 
TATCACTCTG GATTAGATGA CTCAAATCTG ACCAACAGTG ACGAAAAAGT GTCGTCTAAA 
ACCCAAATAA CAATTGATGT GAGAAATTCG CAAGGACCTG CTGAGGACAT TATGGCTAGA 
AATAAGAAGC TGCTAACCAA GGATAATGCT CAACACAAGT GTGAGAACAG CTCTGCTAAC 
CACCACTGCG ATAAAAGTCC TCCTGTCTGC ATCTCAGATA TAATGACTAG TAACACTCGT 
GACAAGCTGT CACCGCTACA GACCAAACCA ACAGATTACA GTCATTCAGA GTCTCAGCCG 
GTTGCCTCAT TGTCTCCTGA GGGTCAGCCC AAAGTGACAG ATGCCAGTGC TAACAGCCAG 
GAAGATGCCT TAATCAGTGT TTCCATCTCA ACCAAATATC AGGCCGAGGT AATCAACACA 
AATGCAATCA CTGAGTCCTA TGGGAACGCT GATTTCCACA ATTCTGAAAC TATGGGAACT 
GTTGCTTTGG ATAAACATCG TGGAGATGCA ACAGATAAAC AGAACAAACA TTACACTGCT 
GTGGAAAACA CAATCAGAAA CGAATCAGCT CAAGGTCAAA AAGCAGAGGT CAGTGGAGTT 
TGTAACAGAA CAGGGGTCAT AAGTGTGTCC ACGCAGATGG AGGAGAGTCG AGAGATGCAT 
TTAGCAGTCA AACAGCCGCC CGTCCACGCC TGCACTCAGA CAGATGGAGA ACAGCTGCAG 
ACGGAGCAAG ATGAAGGTGA TAAAGCGTCT GTAGACTCAC CGGCCCTCTC CCCGACCCCA 
CACACTGTTA CAAACCAACT GCTGCTATCC AGCGTGTTCC CTATGAGTGA CCCAGCACAC 
CTCGCTGAAC GCATCCGGCA AAACCGCAAC CGCATGTCTG CTGCTTATGA TGAGACCGAA 
TATGAACCAT ACGGCCTGCC AGAGGTTGTA ATGAAAGGAT TTGCCGACAT CCCCAGTGGG 
CCAGCCTGTC CGTATGTCCT GCGCAGAGGG CTTCTGGGAA CTGACGCAAT GCCCCTCCCA 
CGCAGAGGAG AAGCCACGCC CCTGGAGGAA GATGACGACA TAGAGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-115E12.6 ( XM_021472747.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328422.1 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X4, mRNA.

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CloneID	ODa145582
Clone ID Related Accession (Same CDS sequence)	XM_021472747.1
Accession Version	XM_021472747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centromere protein F-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394892.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CTGCAGTTTG GAGACGTCCC CAAGCCCTCA 
GCAGGCGGGG CCTCCTCCCC GTTTCCACAG CAACCATATA AATCCCCGCC CCTCCGGCGC 
CACGTCCGCC AATCAGAGCC ATCCACCCCC TCCTCTGTGT TTCCATGGGA ACGTGATGTC 
TTGTGGTCCA CTCCCAAAGG ACGACCGACT TCCTTGGTTT CATCCAGTGA GGTCATGATC 
AAGAGCAGTA ATTGTGGGAT GGAGGACGCC CTGAGGAACG AGATCGATGA GCTGCGTGTG 
CGTGTTTCAG ATCTACAGCG CGAGGCACAG CTGGAGACTG AGCGCATGAA GGATGTGGAG 
TCTCGTCTGG CTCAGGCTAA CAGAGAGATC AGCAGCAAAG AGCAGAGCCT GACGCGCACG 
CAAGAGCAGC TGACCCGCGC ACAGACCCGC AGCACACAGG AAACTGACAG AGCCCAGACA 
GCTGAGCAGA AAGTCAAGCA CCTTCAGGAG GAGCTGAAAT GTCAAAGGCA GAATGCTGAA 
AGCAGTCGCT GCAATGCAGA GCAGCGCAGG AAAGACATGG AGAGAGAACA TCAACGGGAG 
CTGGTGGAGC AGCAGAGAGA GAGGCAGGCT CTAGAGAAAC AGCATCAGCA GGAGAACAAC 
AGACTAAACC AGGAGATCCA GCAGGCCAGA ACTCTTCACA ACACACTGCA GGCTCAGCAT 
GACAAGGTGT GTTTGCAGAA GCAGAGTTTG GAGCGAGATC TGGAGGATGT GAAAGGGAAA 
CTGAAGAACA CTGAGGCTGA CCTGAAAGAA AGTCAGAAGA GAGAGGCACA AACTGAGGCA 
AAACTCACGG AGGCGCTGAG GGAGTGTGAG AGTCTCACTG TTTCTCTGGG GCAGATGAAA 
AAACAGGAGA AAGTGCTACA GGAGGAAGTG AAGCGCTTGA CTGAAGAGCT TGCTGAGGCG 
CTCAAGCTTA TTAAAGAACT GCAAGCTCAG CTGGCCGCTT CTCCTCCACC TGTTGCTGCC 
CCTAACTTTA GCTCAGCTGG AGACTCCTTC TCTCCCTGTG TTTCCCTCCA TCACGACCGC 
TCTTCCCCTT TCAACAACTC CTCTCAGAGA AAAAGAGCCC CTAATACAGG GAGAACAAGG 
GAAGAGGAGA GAATGAAGTA TCCCTCCGGT AGAGAGCCTG GAGAGGGCAT CGACTCAGAG 
CACATTGACA AATTTGAGTC CGAGGAGAAA TCCCAAAAGC GTATAGGAGT CCAAACACTT 
CCAAGTGGCT CTCTGAAACT GACTGAGTTT GATTTAGACA CCTCAGTGAT GGAACAAGAC 
ACGGGTATTG AAGATGTGGA TACGGACTCC TTCACGTCCG ACTCAACCAG GGGCACGATG 
CCTAAAAGTG AATCGGACAT CAGTCGCAGC CTAAAGTTAG ACAATACATC AGGTAAAACA 
GAAAAATATG ACTCTACAAC TCTGAAAGAT CTGAAGAAGC AGAATGCAGA GCTTCAAGAT 
GAGCTTAGAG ATGTGAAATA TGACCTTCAG AAGCGACTTG AGGATCTTGA AACCCAGAGG 
AGAGCAGAAA CTGAGGCCAG GACCAAATTG AAGCAATTGA GTCGCAAACA CTCAACCCAG 
ACAGAGCAGC AACGTGCCAA AGCTCTGGAG CTCAAGGATA GCCTGGTGAA ATTGGAAACT 
CAGCTGGAAC AAGAGAAGAA GGAGAGCTCA AAATTAAAGG AAACACTTCA TGCTCTGCAA 
AGTGAAGCTG AGAACAGGAA GGTGAATGAC CAGGAAGAAG ATTCACAGCT GAAGAAGGCC 
TTGGCTGAAA TGGAGCAAAA GGAGACCATC ATGGAAGAAG AAATGATGGG AATCAAAAAG 
GAACTGCAAG ACCTTCAACT AAAGCTTGCA CAGGAACGGG AGGAGAGGGA GCAGGAGAGA 
TTGGAGGAAA GGAAGCTGAT CAGAAGAGAG GAAGGACTAA AGATTGCACA GCTTCAGGAA 
GAGCTGGACA TTCTTCGAAA GTCTACGGGT TTGGAAGAGA AGATATCAAA AGACAATCTT 
CCCCTCACGT ATTTACATCT AGAACACCAC CAAAACACTG ATCAGAAAGC TGATCTTACC 
GAAAACAAAG ACTTGATTCC TTCTCCAGGA GTTCAAGAGT TTTCTGTGAA TCTTCAGAAC 
ACAATGGTCC GCAATGAAGC TAAAACAATT GAGCTGATTA TGGACCTTGA AGCACAAACT 
AAACCGAAGA CCTCTCCTTC AGATATGAAT AGGGCTCGAG CATTAAGTAC AGAAGCTTCA 
GATTTGGACA ACTCTACGGT TTTAGTTTTG GAGGTGGAAC GCTTGAGAGC TGCGCGAGAC 
AGAGAGTCTG AGAAGGCAAA AGTATCTCAA AGAAAGCTGG AGGAGCTACA GAAGCAAGTG 
ACCACTCAGA CCAAGCAACT CACCCAAGCT TTCGAAAGCC AGAGCAAGCA CATTGACAAC 
TTGTTAAGCG AGTTAGAGGA TAAAGAGTGT GCTCTCCAGA AGCAGGCAGA GGAGCTGCAA 
AAATGCCAGG AGAAAATCTG TTTTCTTGAA GAAAAACAAA CCAGGACGAA TGATCTCTCT 
GCACCTCCTG AACTATCTAC AGAGATCTCA AGAGAACTGA GCTGTGATTC AGTCTTCAGC 
AACACGAGTA TAAGTGACCA TGAAATGTTT AGTGATGATA CACCACCGGT TTTGAAGGAG 
AAGGTCTCAG AACATACTGT ACAGCTTCCT GTTGAAGCCT CTGTTATTCA GAACATTGAT 
CAAACAAACA CTAGTGAGAT TTCTGATAAG CTACAAGCGA CTGAACATTT TAAACAACAG 
TCTGTAGACA CTTCAAATTC TAGCACAGCT TCAGATGCAC CAATAATTTC TGATGTAACT 
GAAGAGAAAT CAAGTTCTGT CTTGCAGGAT GAACAGTTGA ATGTTGTTAA CTGCCAAAAT 
GACAGTATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTTGCTCTA 
CAGGGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAGAAGTT GCAGGACCAA ATCCTGGACC TCTTTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCCACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTTAA AGAGTTTGAG CTGTTCCTCC CCTGCGGTCC TAGCAAACTG 
CACACACAGT CTCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AAAAAGTGTC GTCTAAAACC 
CAAATAACAA TTGATGTGAG AAATTCGCAA GGACCTGCTG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
CACTGCGATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACAGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAA GGTCAAAAAG CAGAGGTCAG TGGAGTTTGT 
AACAGAACAG GGGTCATAAG TGTGTCCACG CAGATGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATCCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328422.1
CDS	320..5407
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAASPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIDKFESEEKSQKRIGVQTLPSGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTGLEEKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTMVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSAPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSEHTVQLPVEASVIQNIDQTNTSEISDKLQATEHFKQQSVDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDSIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQGDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQILDLFEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGPSKLHTQSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSKTQITIDVRNSQGPAEDIMARNKKLLTKDNAQHKCENSSANHHCDKSPPVCISDIMTSNTRDKLSPLQTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVCNRTGVISVSTQMEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_021472747.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472747.1

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CTGCAGTTTG GAGACGTCCC CAAGCCCTCA 
GCAGGCGGGG CCTCCTCCCC GTTTCCACAG CAACCATATA AATCCCCGCC CCTCCGGCGC 
CACGTCCGCC AATCAGAGCC ATCCACCCCC TCCTCTGTGT TTCCATGGGA ACGTGATGTC 
TTGTGGTCCA CTCCCAAAGG ACGACCGACT TCCTTGGTTT CATCCAGTGA GGTCATGATC 
AAGAGCAGTA ATTGTGGGAT GGAGGACGCC CTGAGGAACG AGATCGATGA GCTGCGTGTG 
CGTGTTTCAG ATCTACAGCG CGAGGCACAG CTGGAGACTG AGCGCATGAA GGATGTGGAG 
TCTCGTCTGG CTCAGGCTAA CAGAGAGATC AGCAGCAAAG AGCAGAGCCT GACGCGCACG 
CAAGAGCAGC TGACCCGCGC ACAGACCCGC AGCACACAGG AAACTGACAG AGCCCAGACA 
GCTGAGCAGA AAGTCAAGCA CCTTCAGGAG GAGCTGAAAT GTCAAAGGCA GAATGCTGAA 
AGCAGTCGCT GCAATGCAGA GCAGCGCAGG AAAGACATGG AGAGAGAACA TCAACGGGAG 
CTGGTGGAGC AGCAGAGAGA GAGGCAGGCT CTAGAGAAAC AGCATCAGCA GGAGAACAAC 
AGACTAAACC AGGAGATCCA GCAGGCCAGA ACTCTTCACA ACACACTGCA GGCTCAGCAT 
GACAAGGTGT GTTTGCAGAA GCAGAGTTTG GAGCGAGATC TGGAGGATGT GAAAGGGAAA 
CTGAAGAACA CTGAGGCTGA CCTGAAAGAA AGTCAGAAGA GAGAGGCACA AACTGAGGCA 
AAACTCACGG AGGCGCTGAG GGAGTGTGAG AGTCTCACTG TTTCTCTGGG GCAGATGAAA 
AAACAGGAGA AAGTGCTACA GGAGGAAGTG AAGCGCTTGA CTGAAGAGCT TGCTGAGGCG 
CTCAAGCTTA TTAAAGAACT GCAAGCTCAG CTGGCCGCTT CTCCTCCACC TGTTGCTGCC 
CCTAACTTTA GCTCAGCTGG AGACTCCTTC TCTCCCTGTG TTTCCCTCCA TCACGACCGC 
TCTTCCCCTT TCAACAACTC CTCTCAGAGA AAAAGAGCCC CTAATACAGG GAGAACAAGG 
GAAGAGGAGA GAATGAAGTA TCCCTCCGGT AGAGAGCCTG GAGAGGGCAT CGACTCAGAG 
CACATTGACA AATTTGAGTC CGAGGAGAAA TCCCAAAAGC GTATAGGAGT CCAAACACTT 
CCAAGTGGCT CTCTGAAACT GACTGAGTTT GATTTAGACA CCTCAGTGAT GGAACAAGAC 
ACGGGTATTG AAGATGTGGA TACGGACTCC TTCACGTCCG ACTCAACCAG GGGCACGATG 
CCTAAAAGTG AATCGGACAT CAGTCGCAGC CTAAAGTTAG ACAATACATC AGGTAAAACA 
GAAAAATATG ACTCTACAAC TCTGAAAGAT CTGAAGAAGC AGAATGCAGA GCTTCAAGAT 
GAGCTTAGAG ATGTGAAATA TGACCTTCAG AAGCGACTTG AGGATCTTGA AACCCAGAGG 
AGAGCAGAAA CTGAGGCCAG GACCAAATTG AAGCAATTGA GTCGCAAACA CTCAACCCAG 
ACAGAGCAGC AACGTGCCAA AGCTCTGGAG CTCAAGGATA GCCTGGTGAA ATTGGAAACT 
CAGCTGGAAC AAGAGAAGAA GGAGAGCTCA AAATTAAAGG AAACACTTCA TGCTCTGCAA 
AGTGAAGCTG AGAACAGGAA GGTGAATGAC CAGGAAGAAG ATTCACAGCT GAAGAAGGCC 
TTGGCTGAAA TGGAGCAAAA GGAGACCATC ATGGAAGAAG AAATGATGGG AATCAAAAAG 
GAACTGCAAG ACCTTCAACT AAAGCTTGCA CAGGAACGGG AGGAGAGGGA GCAGGAGAGA 
TTGGAGGAAA GGAAGCTGAT CAGAAGAGAG GAAGGACTAA AGATTGCACA GCTTCAGGAA 
GAGCTGGACA TTCTTCGAAA GTCTACGGGT TTGGAAGAGA AGATATCAAA AGACAATCTT 
CCCCTCACGT ATTTACATCT AGAACACCAC CAAAACACTG ATCAGAAAGC TGATCTTACC 
GAAAACAAAG ACTTGATTCC TTCTCCAGGA GTTCAAGAGT TTTCTGTGAA TCTTCAGAAC 
ACAATGGTCC GCAATGAAGC TAAAACAATT GAGCTGATTA TGGACCTTGA AGCACAAACT 
AAACCGAAGA CCTCTCCTTC AGATATGAAT AGGGCTCGAG CATTAAGTAC AGAAGCTTCA 
GATTTGGACA ACTCTACGGT TTTAGTTTTG GAGGTGGAAC GCTTGAGAGC TGCGCGAGAC 
AGAGAGTCTG AGAAGGCAAA AGTATCTCAA AGAAAGCTGG AGGAGCTACA GAAGCAAGTG 
ACCACTCAGA CCAAGCAACT CACCCAAGCT TTCGAAAGCC AGAGCAAGCA CATTGACAAC 
TTGTTAAGCG AGTTAGAGGA TAAAGAGTGT GCTCTCCAGA AGCAGGCAGA GGAGCTGCAA 
AAATGCCAGG AGAAAATCTG TTTTCTTGAA GAAAAACAAA CCAGGACGAA TGATCTCTCT 
GCACCTCCTG AACTATCTAC AGAGATCTCA AGAGAACTGA GCTGTGATTC AGTCTTCAGC 
AACACGAGTA TAAGTGACCA TGAAATGTTT AGTGATGATA CACCACCGGT TTTGAAGGAG 
AAGGTCTCAG AACATACTGT ACAGCTTCCT GTTGAAGCCT CTGTTATTCA GAACATTGAT 
CAAACAAACA CTAGTGAGAT TTCTGATAAG CTACAAGCGA CTGAACATTT TAAACAACAG 
TCTGTAGACA CTTCAAATTC TAGCACAGCT TCAGATGCAC CAATAATTTC TGATGTAACT 
GAAGAGAAAT CAAGTTCTGT CTTGCAGGAT GAACAGTTGA ATGTTGTTAA CTGCCAAAAT 
GACAGTATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTTGCTCTA 
CAGGGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAGAAGTT GCAGGACCAA ATCCTGGACC TCTTTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCCACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTTAA AGAGTTTGAG CTGTTCCTCC CCTGCGGTCC TAGCAAACTG 
CACACACAGT CTCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AAAAAGTGTC GTCTAAAACC 
CAAATAACAA TTGATGTGAG AAATTCGCAA GGACCTGCTG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
CACTGCGATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACAGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAA GGTCAAAAAG CAGAGGTCAG TGGAGTTTGT 
AACAGAACAG GGGTCATAAG TGTGTCCACG CAGATGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATCCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-115E12.6 ( XM_001344567.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001344603.3 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa19387
Clone ID Related Accession (Same CDS sequence)	XM_001344567.7 , XM_021481112.1 , XM_005157164.3
Accession Version	XM_001344567.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centromere protein F-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001344567.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTCCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG AGAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTAAAGG AGTCCAAACA 
CTTCCAATTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCAGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTA 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG AGTTTGGAGG TGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATAG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTGAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAACTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACTAGGAC GAATGATCTC 
TCTGTACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACTTAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAG TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAAAA TTTTAAACAA 
CAGTCTCTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTAGCTCTA 
CAGAGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAAAAGTT GCAGGACCAA ATCCGGGACC TCTCTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCAACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTAAA AGAGTTTGAG CTGTTCCTTC CCTGCGGTTC TAGAAAACTG 
CACACACACT CCCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AGAAAGTGTC GTCTGAAACC 
CAAATAACAA TTGATGTGGA AAATTCGCAA GGACCTGCAG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
TACTGCCATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACTGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAG GGTCAAAAAG CAGAGGTCAG TGGAGTTTAT 
AAGAGAACAG GGGTCATAAG TGTGTCCACG CAGACGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATTCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001344603.3
CDS	303..5390
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAAPPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIEKFESEEKSQKRKGVQTLPIGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTSLEVKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTIVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSVPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSELTVQLPVEASVSQNIDQTNTSEISDKLQATENFKQQSLDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQRDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQIRDLSEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGSRKLHTHSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSETQITIDVENSQGPAEDIMARNKKLLTKDNAQHKCENSSANHYCHKSPPVCISDIMTSNTRDKLSPLLTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVYKRTGVISVSTQTEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_001344567.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001344567.7

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTCCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG AGAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTAAAGG AGTCCAAACA 
CTTCCAATTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCAGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTA 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG AGTTTGGAGG TGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATAG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTGAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAACTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACTAGGAC GAATGATCTC 
TCTGTACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACTTAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAG TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAAAA TTTTAAACAA 
CAGTCTCTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTAGCTCTA 
CAGAGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAAAAGTT GCAGGACCAA ATCCGGGACC TCTCTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCAACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTAAA AGAGTTTGAG CTGTTCCTTC CCTGCGGTTC TAGAAAACTG 
CACACACACT CCCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AGAAAGTGTC GTCTGAAACC 
CAAATAACAA TTGATGTGGA AAATTCGCAA GGACCTGCAG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
TACTGCCATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACTGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAG GGTCAAAAAG CAGAGGTCAG TGGAGTTTAT 
AAGAGAACAG GGGTCATAAG TGTGTCCACG CAGACGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATTCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-115E12.6 ( XM_021481112.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336787.1 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X1, mRNA.

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CloneID	ODa19387
Clone ID Related Accession (Same CDS sequence)	XM_001344567.7 , XM_021481112.1 , XM_005157164.3
Accession Version	XM_021481112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centromere protein F-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTCCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG AGAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTAAAGG AGTCCAAACA 
CTTCCAATTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCAGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTA 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG AGTTTGGAGG TGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATAG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTGAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAACTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACTAGGAC GAATGATCTC 
TCTGTACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACTTAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAG TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAAAA TTTTAAACAA 
CAGTCTCTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTAGCTCTA 
CAGAGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAAAAGTT GCAGGACCAA ATCCGGGACC TCTCTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCAACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTAAA AGAGTTTGAG CTGTTCCTTC CCTGCGGTTC TAGAAAACTG 
CACACACACT CCCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AGAAAGTGTC GTCTGAAACC 
CAAATAACAA TTGATGTGGA AAATTCGCAA GGACCTGCAG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
TACTGCCATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACTGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAG GGTCAAAAAG CAGAGGTCAG TGGAGTTTAT 
AAGAGAACAG GGGTCATAAG TGTGTCCACG CAGACGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATTCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336787.1
CDS	284..5371
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAAPPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIEKFESEEKSQKRKGVQTLPIGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTSLEVKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTIVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSVPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSELTVQLPVEASVSQNIDQTNTSEISDKLQATENFKQQSLDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQRDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQIRDLSEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGSRKLHTHSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSETQITIDVENSQGPAEDIMARNKKLLTKDNAQHKCENSSANHYCHKSPPVCISDIMTSNTRDKLSPLLTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVYKRTGVISVSTQTEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_021481112.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481112.1

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTCCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG AGAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTAAAGG AGTCCAAACA 
CTTCCAATTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCAGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTA 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG AGTTTGGAGG TGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATAG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTGAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAACTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACTAGGAC GAATGATCTC 
TCTGTACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACTTAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAG TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAAAA TTTTAAACAA 
CAGTCTCTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTAGCTCTA 
CAGAGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAAAAGTT GCAGGACCAA ATCCGGGACC TCTCTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCAACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTAAA AGAGTTTGAG CTGTTCCTTC CCTGCGGTTC TAGAAAACTG 
CACACACACT CCCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AGAAAGTGTC GTCTGAAACC 
CAAATAACAA TTGATGTGGA AAATTCGCAA GGACCTGCAG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
TACTGCCATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACTGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAG GGTCAAAAAG CAGAGGTCAG TGGAGTTTAT 
AAGAGAACAG GGGTCATAAG TGTGTCCACG CAGACGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATTCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-115E12.6 ( XM_005157164.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157221.1 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X1, mRNA.

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CloneID	ODa19387
Clone ID Related Accession (Same CDS sequence)	XM_001344567.7 , XM_021481112.1 , XM_005157164.3
Accession Version	XM_005157164.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centromere protein F-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTCCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG AGAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTAAAGG AGTCCAAACA 
CTTCCAATTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCAGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTA 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG AGTTTGGAGG TGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATAG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTGAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAACTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACTAGGAC GAATGATCTC 
TCTGTACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACTTAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAG TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAAAA TTTTAAACAA 
CAGTCTCTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTAGCTCTA 
CAGAGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAAAAGTT GCAGGACCAA ATCCGGGACC TCTCTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCAACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTAAA AGAGTTTGAG CTGTTCCTTC CCTGCGGTTC TAGAAAACTG 
CACACACACT CCCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AGAAAGTGTC GTCTGAAACC 
CAAATAACAA TTGATGTGGA AAATTCGCAA GGACCTGCAG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
TACTGCCATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACTGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAG GGTCAAAAAG CAGAGGTCAG TGGAGTTTAT 
AAGAGAACAG GGGTCATAAG TGTGTCCACG CAGACGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATTCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157221.1
CDS	284..5371
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAAPPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIEKFESEEKSQKRKGVQTLPIGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTSLEVKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTIVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSVPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSELTVQLPVEASVSQNIDQTNTSEISDKLQATENFKQQSLDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQRDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQIRDLSEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGSRKLHTHSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSETQITIDVENSQGPAEDIMARNKKLLTKDNAQHKCENSSANHYCHKSPPVCISDIMTSNTRDKLSPLLTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVYKRTGVISVSTQTEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_005157164.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157164.3

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CAGCTGCAGT TTGGAGACGT CCCCAAGCCC 
TCAGCAGGCG GGGCCTCCTC CCCGTTTCCA CAGCAACCAT ATAAATCCCC GCCCCTCCGG 
CGCCACGTCC GCCAATCAGA GCCATCCACC CCCTCCTCTG TGTTTCCATG GGAACGTGAT 
GTCTTGTGGT CCACTCCCAA AGGACGACCG ACTTCCTTGG TTTCATCCAG TGAGGTCATG 
ATCAAGAGCA GTAATTGTGG GATGGAGGAC GCCCTGAGGA ACGAGATCGA TGAGCTGCGT 
GTGCGTGTTT CAGATCTACA GCGCGAGGCA CAGCTGGAGA CTGAGCGCAT GAAGGATGTG 
GAGTCTCGTC TGGCTCAGGC TAACAGAGAG ATCAGCAGCA AAGAGCAGAG CCTGACGCGC 
ACGCAAGAGC AGCTGACCCG CGCACAGACC CGCAGCACAC AGGAAACTGA CAGAGCCCAG 
ACAGCTGAGC AGAAAGTCAA GCACCTTCAG GAGGAGCTGA AATGTCAAAG GCAGAATGCT 
GAAAGCAGTC GCTGCAATGC AGAGCAGCGC AGGAAAGACA TGGAGAGAGA ACATCAACGG 
GAGCTGGTGG AGCAGCAGAG AGAGAGGCAG GCTCTAGAGA AACAGCATCA GCAGGAGAAC 
AACAGACTAA ACCAGGAGAT CCAGCAGGCC AGAACTCTTC ACAACACACT GCAGGCTCAG 
CATGACAAGG TGTGTTTGCA GAAGCAGAGT TTGGAGCGAG ATCTGGAGGA TGTGAAAGGG 
AAACTGAAGA ACACTGAGGC TGACCTGAAA GAAAGTCAGA AGAGAGAGGC ACAAACTGAG 
GCAAAACTCA CGGAGGCGCT GAGGGAGTGT GAGAGTCTCA CTGTTTCTCT GGGGCAGATG 
AAAAAACAGG AGAAAGTGCT ACAGGAGGAA GTGAAGCGCT TGACTGAAGA GCTTGCTGAG 
GCGCTCAAGC TTATTAAAGA ACTGCAAGCT CAGCTGGCCG CTCCTCCTCC ACCTGTTGCT 
GCCCCTAACT TTAGCTCAGC TGGAGACTCC TTCTCTCCCT GTGTTTCCCT CCATCACGAC 
CGCTCTTCCC CTTTCAACAA CTCCTCTCAG AGAAAAAGAG CCCCTAATAC AGGGAGAACA 
AGGGAAGAGG AGAGAATGAA GTATCCCTCC GGTAGAGAGC CTGGAGAGGG CATCGACTCA 
GAGCACATTG AGAAATTTGA GTCCGAGGAG AAATCCCAAA AGCGTAAAGG AGTCCAAACA 
CTTCCAATTG GCTCTCTGAA ACTGACTGAG TTTGATTTAG ACACCTCAGT GATGGAACAA 
GACACGGGTA TTGAAGATGT GGATACGGAC TCCTTCACGT CCGACTCAAC CAGGGGCACG 
ATGCCTAAAA GTGAATCAGA CATCAGTCGC AGCCTAAAGT TAGACAATAC ATCAGGTAAA 
ACAGAAAAAT ATGACTCTAC AACTCTGAAA GATCTGAAGA AGCAGAATGC AGAGCTTCAA 
GATGAGCTTA GAGATGTGAA ATATGACCTT CAGAAGCGAC TTGAGGATCT TGAAACCCAG 
AGGAGAGCAG AAACTGAGGC CAGGACCAAA TTGAAGCAAT TGAGTCGCAA ACACTCAACC 
CAGACAGAGC AGCAACGTGC CAAAGCTCTG GAGCTCAAGG ATAGCCTGGT GAAATTGGAA 
ACTCAGCTGG AACAAGAGAA GAAGGAGAGC TCAAAATTAA AGGAAACACT TCATGCTCTA 
CAAAGTGAAG CTGAGAACAG GAAGGTGAAT GACCAGGAAG AAGATTCACA GCTGAAGAAG 
GCCTTGGCTG AAATGGAGCA AAAGGAGACC ATCATGGAAG AAGAAATGAT GGGAATCAAA 
AAGGAACTGC AAGACCTTCA ACTAAAGCTT GCACAGGAAC GGGAGGAGAG GGAGCAGGAG 
AGATTGGAGG AAAGGAAGCT GATCAGAAGA GAGGAAGGAC TAAAGATTGC ACAGCTTCAG 
GAAGAGCTGG ACATTCTTCG AAAGTCTACG AGTTTGGAGG TGAAGATATC AAAAGACAAT 
CTTCCCCTCA CGTATTTACA TCTAGAACAC CACCAAAACA CTGATCAGAA AGCTGATCTT 
ACCGAAAACA AAGACTTGAT TCCTTCTCCA GGAGTTCAAG AGTTTTCTGT GAATCTTCAG 
AACACAATAG TCCGCAATGA AGCTAAAACA ATTGAGCTGA TTATGGACCT TGAAGCACAA 
ACTAAACCGA AGACCTCTCC TTCAGATATG AATAGGGCTC GAGCATTGAG TACAGAAGCT 
TCAGATTTGG ACAACTCTAC GGTTTTAGTT TTGGAGGTGG AACGCTTGAG AGCTGCGCGA 
GACAGAGAGT CTGAGAAGGC AAAAGTATCT CAAAGAAAGC TGGAGGAGCT ACAGAAGCAA 
GTGACCACTC AGACCAAGCA ACTCACCCAA GCTTTCGAAA GCCAGAGCAA GCACATTGAC 
AACTTGTTAA GCGAGTTAGA GGATAAAGAG TGTGCTCTCC AGAAGCAGGC AGAGGAACTG 
CAAAAATGCC AGGAGAAAAT CTGTTTTCTT GAAGAAAAAC AAACTAGGAC GAATGATCTC 
TCTGTACCTC CTGAACTATC TACAGAGATC TCAAGAGAAC TGAGCTGTGA TTCAGTCTTC 
AGCAACACGA GTATAAGTGA CCATGAAATG TTTAGTGATG ATACACCACC GGTTTTGAAG 
GAGAAGGTCT CAGAACTTAC TGTACAGCTT CCTGTTGAAG CCTCTGTTAG TCAGAACATT 
GATCAAACAA ACACTAGTGA GATTTCTGAT AAGCTACAAG CGACTGAAAA TTTTAAACAA 
CAGTCTCTAG ACACTTCAAA TTCTAGCACA GCTTCAGATG CACCAATAAT TTCTGATGTA 
ACTGAAGAGA AATCAAGTTC TGTCTTGCAG GATGAACAGT TGAATGTTGT TAACTGCCAA 
AATGACATTC CAGAACATTC AATTCATGAT ATCAAGGAGC TCGAACGAGT TACAAAGGAA 
TTACAAGCAG CTCAACTCGA GCTAAATGTG ATGAAGGCTC AGAATTCAGA GCTAGCTCTA 
CAGAGAGACA TGATAAAAGA TAAGCTACTG AATGTTCAGC AAGAGAATCA AGAGTTGAAA 
TCAACTCTGA AACACTTGTG TGAAGACACT GGTGCTAAAC AAGATGGTGC CTTAAATTTA 
GCGAACTCTA AAAACAAATC GTTGTTGAGT TCAAATGAAG GATTGCAAAG CATCTCCCCA 
TTAGGGAAAG AGAAATTGGT CGTTCAGCAT GGAAACTCTG AAGCGGAGGT TCAGTCTCTT 
CAGGAGCAGA TCCAAAAGTT GCAGGACCAA ATCCGGGACC TCTCTGAACA AAACAGGACT 
CAGGCTGAAC AGCTGGAGCT CTGGAAGCTG TCAACTGTGG AAGAAACAGC GGGCAACAAC 
TCACCCTCTA TTGTGCTAAA AGAGTTTGAG CTGTTCCTTC CCTGCGGTTC TAGAAAACTG 
CACACACACT CCCAAACCAG GACCATGATG CACCAGTGCG ATGTCAGAGA TGTGACTTAT 
CACTCTGGAT TAGATGACTC AAATCTGACC AACAGTGACG AGAAAGTGTC GTCTGAAACC 
CAAATAACAA TTGATGTGGA AAATTCGCAA GGACCTGCAG AGGACATTAT GGCTAGAAAT 
AAGAAGCTGC TAACCAAGGA TAATGCTCAA CACAAGTGTG AGAACAGCTC TGCTAACCAC 
TACTGCCATA AAAGTCCTCC TGTCTGCATC TCAGATATAA TGACTAGTAA CACTCGTGAC 
AAGCTGTCAC CGCTACTGAC CAAACCAACA GATTACAGTC ATTCAGAGTC TCAGCCGGTT 
GCCTCATTGT CTCCTGAGGG TCAGCCCAAA GTGACAGATG CCAGTGCTAA CAGCCAGGAA 
GATGCCTTAA TCAGTGTTTC CATCTCAACC AAATATCAGG CCGAGGTAAT CAACACAAAT 
GCAATCACTG AGTCCTATGG GAACGCTGAT TTCCACAATT CTGAAACTAT GGGAACTGTT 
GCTTTGGATA AACATCGTGG AGATGCAACA GATAAACAGA ACAAACATTA CACTGCTGTG 
GAAAACACAA TCAGAAACGA ATCAGCTCAG GGTCAAAAAG CAGAGGTCAG TGGAGTTTAT 
AAGAGAACAG GGGTCATAAG TGTGTCCACG CAGACGGAGG AGAGTCGAGA GATGCATTTA 
GCAGTCAAAC AGCCGCCCGT CCACGCCTGC ACTCAGACAG ATGGAGAACA GCTGCAGACG 
GAGCAAGATG AAGGTGATAA AGCGTCTGTA GACTCACCGG CCCTCTCCCC GACCCCACAC 
ACTGTTACAA ACCAACTGCT GCTATCCAGC GTGTTCCCTA TGAGTGACCC AGCACACCTC 
GCTGAACGCA TCCGGCAAAA CCGCAACCGC ATGTCTGCTG CTTATGATGA GACCGAATAT 
GAACCATACG GCCTGCCAGA GGTTGTAATG AAAGGATTTG CCGACATTCC CAGTGGGCCA 
GCCTGTCCGT ATGTCCTGCG CAGAGGGCTT CTGGGAACTG ACGCAATGCC CCTCCCACGC 
AGAGGAGAAG CCACGCCCCT GGAGGAAGAT GACGACATAG AGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-115E12.6 ( XM_005157165.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157222.1 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X3, mRNA.

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CloneID	ODa19388
Clone ID Related Accession (Same CDS sequence)	XM_005157165.3 , XM_021481113.1
Accession Version	XM_005157165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centromere protein F-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CTGCAGTTTG GAGACGTCCC CAAGCCCTCA 
GCAGGCGGGG CCTCCTCCCC GTTTCCACAG CAACCATATA AATCCCCGCC CCTCCGGCGC 
CACGTCCGCC AATCAGAGCC ATCCACCCCC TCCTCTGTGT TTCCATGGGA ACGTGATGTC 
TTGTGGTCCA CTCCCAAAGG ACGACCGACT TCCTTGGTTT CATCCAGTGA GGTCATGATC 
AAGAGCAGTA ATTGTGGGAT GGAGGACGCC CTGAGGAACG AGATCGATGA GCTGCGTGTG 
CGTGTTTCAG ATCTACAGCG CGAGGCACAG CTGGAGACTG AGCGCATGAA GGATGTGGAG 
TCTCGTCTGG CTCAGGCTAA CAGAGAGATC AGCAGCAAAG AGCAGAGCCT GACGCGCACG 
CAAGAGCAGC TGACCCGCGC ACAGACCCGC AGCACACAGG AAACTGACAG AGCCCAGACA 
GCTGAGCAGA AAGTCAAGCA CCTTCAGGAG GAGCTGAAAT GTCAAAGGCA GAATGCTGAA 
AGCAGTCGCT GCAATGCAGA GCAGCGCAGG AAAGACATGG AGAGAGAACA TCAACGGGAG 
CTGGTGGAGC AGCAGAGAGA GAGGCAGGCT CTAGAGAAAC AGCATCAGCA GGAGAACAAC 
AGACTAAACC AGGAGATCCA GCAGGCCAGA ACTCTTCACA ACACACTGCA GGCTCAGCAT 
GACAAGGTGT GTTTGCAGAA GCAGAGTTTG GAGCGAGATC TGGAGGATGT GAAAGGGAAA 
CTGAAGAACA CTGAGGCTGA CCTGAAAGAA AGTCAGAAGA GAGAGGCACA AACTGAGGCA 
AAACTCACGG AGGCGCTGAG GGAGTGTGAG AGTCTCACTG TTTCTCTGGG GCAGATGAAA 
AAACAGGAGA AAGTGCTACA GGAGGAAGTG AAGCGCTTGA CTGAAGAGCT TGCTGAGGCG 
CTCAAGCTTA TTAAAGAACT GCAAGCTCAG CTGGCCGCTC CTCCTCCACC TGTTGCTGCC 
CCTAACTTTA GCTCAGCTGG AGACTCCTTC TCTCCCTGTG TTTCCCTCCA TCACGACCGC 
TCTTCCCCTT TCAACAACTC CTCTCAGAGA AAAAGAGCCC CTAATACAGG GAGAACAAGG 
GAAGAGGAGA GAATGAAGTA TCCCTCCGGT AGAGAGCCTG GAGAGGGCAT CGACTCAGAG 
CACATTGAGA AATTTGAGTC CGAGGAGAAA TCCCAAAAGC GTAAAGGAGT CCAAACACTT 
CCAATTGGCT CTCTGAAACT GACTGAGTTT GATTTAGACA CCTCAGTGAT GGAACAAGAC 
ACGGGTATTG AAGATGTGGA TACGGACTCC TTCACGTCCG ACTCAACCAG GGGCACGATG 
CCTAAAAGTG AATCAGACAT CAGTCGCAGC CTAAAGTTAG ACAATACATC AGGTAAAACA 
GAAAAATATG ACTCTACAAC TCTGAAAGAT CTGAAGAAGC AGAATGCAGA GCTTCAAGAT 
GAGCTTAGAG ATGTGAAATA TGACCTTCAG AAGCGACTTG AGGATCTTGA AACCCAGAGG 
AGAGCAGAAA CTGAGGCCAG GACCAAATTG AAGCAATTGA GTCGCAAACA CTCAACCCAG 
ACAGAGCAGC AACGTGCCAA AGCTCTGGAG CTCAAGGATA GCCTGGTGAA ATTGGAAACT 
CAGCTGGAAC AAGAGAAGAA GGAGAGCTCA AAATTAAAGG AAACACTTCA TGCTCTACAA 
AGTGAAGCTG AGAACAGGAA GGTGAATGAC CAGGAAGAAG ATTCACAGCT GAAGAAGGCC 
TTGGCTGAAA TGGAGCAAAA GGAGACCATC ATGGAAGAAG AAATGATGGG AATCAAAAAG 
GAACTGCAAG ACCTTCAACT AAAGCTTGCA CAGGAACGGG AGGAGAGGGA GCAGGAGAGA 
TTGGAGGAAA GGAAGCTGAT CAGAAGAGAG GAAGGACTAA AGATTGCACA GCTTCAGGAA 
GAGCTGGACA TTCTTCGAAA GTCTACGAGT TTGGAGGTGA AGATATCAAA AGACAATCTT 
CCCCTCACGT ATTTACATCT AGAACACCAC CAAAACACTG ATCAGAAAGC TGATCTTACC 
GAAAACAAAG ACTTGATTCC TTCTCCAGGA GTTCAAGAGT TTTCTGTGAA TCTTCAGAAC 
ACAATAGTCC GCAATGAAGC TAAAACAATT GAGCTGATTA TGGACCTTGA AGCACAAACT 
AAACCGAAGA CCTCTCCTTC AGATATGAAT AGGGCTCGAG CATTGAGTAC AGAAGCTTCA 
GATTTGGACA ACTCTACGGT TTTAGTTTTG GAGGTGGAAC GCTTGAGAGC TGCGCGAGAC 
AGAGAGTCTG AGAAGGCAAA AGTATCTCAA AGAAAGCTGG AGGAGCTACA GAAGCAAGTG 
ACCACTCAGA CCAAGCAACT CACCCAAGCT TTCGAAAGCC AGAGCAAGCA CATTGACAAC 
TTGTTAAGCG AGTTAGAGGA TAAAGAGTGT GCTCTCCAGA AGCAGGCAGA GGAACTGCAA 
AAATGCCAGG AGAAAATCTG TTTTCTTGAA GAAAAACAAA CTAGGACGAA TGATCTCTCT 
GTACCTCCTG AACTATCTAC AGAGATCTCA AGAGAACTGA GCTGTGATTC AGTCTTCAGC 
AACACGAGTA TAAGTGACCA TGAAATGTTT AGTGATGATA CACCACCGGT TTTGAAGGAG 
AAGGTCTCAG AACTTACTGT ACAGCTTCCT GTTGAAGCCT CTGTTAGTCA GAACATTGAT 
CAAACAAACA CTAGTGAGAT TTCTGATAAG CTACAAGCGA CTGAAAATTT TAAACAACAG 
TCTCTAGACA CTTCAAATTC TAGCACAGCT TCAGATGCAC CAATAATTTC TGATGTAACT 
GAAGAGAAAT CAAGTTCTGT CTTGCAGGAT GAACAGTTGA ATGTTGTTAA CTGCCAAAAT 
GACATTCCAG AACATTCAAT TCATGATATC AAGGAGCTCG AACGAGTTAC AAAGGAATTA 
CAAGCAGCTC AACTCGAGCT AAATGTGATG AAGGCTCAGA ATTCAGAGCT AGCTCTACAG 
AGAGACATGA TAAAAGATAA GCTACTGAAT GTTCAGCAAG AGAATCAAGA GTTGAAATCA 
ACTCTGAAAC ACTTGTGTGA AGACACTGGT GCTAAACAAG ATGGTGCCTT AAATTTAGCG 
AACTCTAAAA ACAAATCGTT GTTGAGTTCA AATGAAGGAT TGCAAAGCAT CTCCCCATTA 
GGGAAAGAGA AATTGGTCGT TCAGCATGGA AACTCTGAAG CGGAGGTTCA GTCTCTTCAG 
GAGCAGATCC AAAAGTTGCA GGACCAAATC CGGGACCTCT CTGAACAAAA CAGGACTCAG 
GCTGAACAGC TGGAGCTCTG GAAGCTGTCA ACTGTGGAAG AAACAGCGGG CAACAACTCA 
CCCTCTATTG TGCTAAAAGA GTTTGAGCTG TTCCTTCCCT GCGGTTCTAG AAAACTGCAC 
ACACACTCCC AAACCAGGAC CATGATGCAC CAGTGCGATG TCAGAGATGT GACTTATCAC 
TCTGGATTAG ATGACTCAAA TCTGACCAAC AGTGACGAGA AAGTGTCGTC TGAAACCCAA 
ATAACAATTG ATGTGGAAAA TTCGCAAGGA CCTGCAGAGG ACATTATGGC TAGAAATAAG 
AAGCTGCTAA CCAAGGATAA TGCTCAACAC AAGTGTGAGA ACAGCTCTGC TAACCACTAC 
TGCCATAAAA GTCCTCCTGT CTGCATCTCA GATATAATGA CTAGTAACAC TCGTGACAAG 
CTGTCACCGC TACTGACCAA ACCAACAGAT TACAGTCATT CAGAGTCTCA GCCGGTTGCC 
TCATTGTCTC CTGAGGGTCA GCCCAAAGTG ACAGATGCCA GTGCTAACAG CCAGGAAGAT 
GCCTTAATCA GTGTTTCCAT CTCAACCAAA TATCAGGCCG AGGTAATCAA CACAAATGCA 
ATCACTGAGT CCTATGGGAA CGCTGATTTC CACAATTCTG AAACTATGGG AACTGTTGCT 
TTGGATAAAC ATCGTGGAGA TGCAACAGAT AAACAGAACA AACATTACAC TGCTGTGGAA 
AACACAATCA GAAACGAATC AGCTCAGGGT CAAAAAGCAG AGGTCAGTGG AGTTTATAAG 
AGAACAGGGG TCATAAGTGT GTCCACGCAG ACGGAGGAGA GTCGAGAGAT GCATTTAGCA 
GTCAAACAGC CGCCCGTCCA CGCCTGCACT CAGACAGATG GAGAACAGCT GCAGACGGAG 
CAAGATGAAG GTGATAAAGC GTCTGTAGAC TCACCGGCCC TCTCCCCGAC CCCACACACT 
GTTACAAACC AACTGCTGCT ATCCAGCGTG TTCCCTATGA GTGACCCAGC ACACCTCGCT 
GAACGCATCC GGCAAAACCG CAACCGCATG TCTGCTGCTT ATGATGAGAC CGAATATGAA 
CCATACGGCC TGCCAGAGGT TGTAATGAAA GGATTTGCCG ACATTCCCAG TGGGCCAGCC 
TGTCCGTATG TCCTGCGCAG AGGGCTTCTG GGAACTGACG CAATGCCCCT CCCACGCAGA 
GGAGAAGCCA CGCCCCTGGA GGAAGATGAC GACATAGAGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157222.1
CDS	284..5368
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAAPPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIEKFESEEKSQKRKGVQTLPIGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTSLEVKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTIVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSVPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSELTVQLPVEASVSQNIDQTNTSEISDKLQATENFKQQSLDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQRDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQIRDLSEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGSRKLHTHSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSETQITIDVENSQGPAEDIMARNKKLLTKDNAQHKCENSSANHYCHKSPPVCISDIMTSNTRDKLSPLLTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVYKRTGVISVSTQTEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_005157165.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157165.3

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CTGCAGTTTG GAGACGTCCC CAAGCCCTCA 
GCAGGCGGGG CCTCCTCCCC GTTTCCACAG CAACCATATA AATCCCCGCC CCTCCGGCGC 
CACGTCCGCC AATCAGAGCC ATCCACCCCC TCCTCTGTGT TTCCATGGGA ACGTGATGTC 
TTGTGGTCCA CTCCCAAAGG ACGACCGACT TCCTTGGTTT CATCCAGTGA GGTCATGATC 
AAGAGCAGTA ATTGTGGGAT GGAGGACGCC CTGAGGAACG AGATCGATGA GCTGCGTGTG 
CGTGTTTCAG ATCTACAGCG CGAGGCACAG CTGGAGACTG AGCGCATGAA GGATGTGGAG 
TCTCGTCTGG CTCAGGCTAA CAGAGAGATC AGCAGCAAAG AGCAGAGCCT GACGCGCACG 
CAAGAGCAGC TGACCCGCGC ACAGACCCGC AGCACACAGG AAACTGACAG AGCCCAGACA 
GCTGAGCAGA AAGTCAAGCA CCTTCAGGAG GAGCTGAAAT GTCAAAGGCA GAATGCTGAA 
AGCAGTCGCT GCAATGCAGA GCAGCGCAGG AAAGACATGG AGAGAGAACA TCAACGGGAG 
CTGGTGGAGC AGCAGAGAGA GAGGCAGGCT CTAGAGAAAC AGCATCAGCA GGAGAACAAC 
AGACTAAACC AGGAGATCCA GCAGGCCAGA ACTCTTCACA ACACACTGCA GGCTCAGCAT 
GACAAGGTGT GTTTGCAGAA GCAGAGTTTG GAGCGAGATC TGGAGGATGT GAAAGGGAAA 
CTGAAGAACA CTGAGGCTGA CCTGAAAGAA AGTCAGAAGA GAGAGGCACA AACTGAGGCA 
AAACTCACGG AGGCGCTGAG GGAGTGTGAG AGTCTCACTG TTTCTCTGGG GCAGATGAAA 
AAACAGGAGA AAGTGCTACA GGAGGAAGTG AAGCGCTTGA CTGAAGAGCT TGCTGAGGCG 
CTCAAGCTTA TTAAAGAACT GCAAGCTCAG CTGGCCGCTC CTCCTCCACC TGTTGCTGCC 
CCTAACTTTA GCTCAGCTGG AGACTCCTTC TCTCCCTGTG TTTCCCTCCA TCACGACCGC 
TCTTCCCCTT TCAACAACTC CTCTCAGAGA AAAAGAGCCC CTAATACAGG GAGAACAAGG 
GAAGAGGAGA GAATGAAGTA TCCCTCCGGT AGAGAGCCTG GAGAGGGCAT CGACTCAGAG 
CACATTGAGA AATTTGAGTC CGAGGAGAAA TCCCAAAAGC GTAAAGGAGT CCAAACACTT 
CCAATTGGCT CTCTGAAACT GACTGAGTTT GATTTAGACA CCTCAGTGAT GGAACAAGAC 
ACGGGTATTG AAGATGTGGA TACGGACTCC TTCACGTCCG ACTCAACCAG GGGCACGATG 
CCTAAAAGTG AATCAGACAT CAGTCGCAGC CTAAAGTTAG ACAATACATC AGGTAAAACA 
GAAAAATATG ACTCTACAAC TCTGAAAGAT CTGAAGAAGC AGAATGCAGA GCTTCAAGAT 
GAGCTTAGAG ATGTGAAATA TGACCTTCAG AAGCGACTTG AGGATCTTGA AACCCAGAGG 
AGAGCAGAAA CTGAGGCCAG GACCAAATTG AAGCAATTGA GTCGCAAACA CTCAACCCAG 
ACAGAGCAGC AACGTGCCAA AGCTCTGGAG CTCAAGGATA GCCTGGTGAA ATTGGAAACT 
CAGCTGGAAC AAGAGAAGAA GGAGAGCTCA AAATTAAAGG AAACACTTCA TGCTCTACAA 
AGTGAAGCTG AGAACAGGAA GGTGAATGAC CAGGAAGAAG ATTCACAGCT GAAGAAGGCC 
TTGGCTGAAA TGGAGCAAAA GGAGACCATC ATGGAAGAAG AAATGATGGG AATCAAAAAG 
GAACTGCAAG ACCTTCAACT AAAGCTTGCA CAGGAACGGG AGGAGAGGGA GCAGGAGAGA 
TTGGAGGAAA GGAAGCTGAT CAGAAGAGAG GAAGGACTAA AGATTGCACA GCTTCAGGAA 
GAGCTGGACA TTCTTCGAAA GTCTACGAGT TTGGAGGTGA AGATATCAAA AGACAATCTT 
CCCCTCACGT ATTTACATCT AGAACACCAC CAAAACACTG ATCAGAAAGC TGATCTTACC 
GAAAACAAAG ACTTGATTCC TTCTCCAGGA GTTCAAGAGT TTTCTGTGAA TCTTCAGAAC 
ACAATAGTCC GCAATGAAGC TAAAACAATT GAGCTGATTA TGGACCTTGA AGCACAAACT 
AAACCGAAGA CCTCTCCTTC AGATATGAAT AGGGCTCGAG CATTGAGTAC AGAAGCTTCA 
GATTTGGACA ACTCTACGGT TTTAGTTTTG GAGGTGGAAC GCTTGAGAGC TGCGCGAGAC 
AGAGAGTCTG AGAAGGCAAA AGTATCTCAA AGAAAGCTGG AGGAGCTACA GAAGCAAGTG 
ACCACTCAGA CCAAGCAACT CACCCAAGCT TTCGAAAGCC AGAGCAAGCA CATTGACAAC 
TTGTTAAGCG AGTTAGAGGA TAAAGAGTGT GCTCTCCAGA AGCAGGCAGA GGAACTGCAA 
AAATGCCAGG AGAAAATCTG TTTTCTTGAA GAAAAACAAA CTAGGACGAA TGATCTCTCT 
GTACCTCCTG AACTATCTAC AGAGATCTCA AGAGAACTGA GCTGTGATTC AGTCTTCAGC 
AACACGAGTA TAAGTGACCA TGAAATGTTT AGTGATGATA CACCACCGGT TTTGAAGGAG 
AAGGTCTCAG AACTTACTGT ACAGCTTCCT GTTGAAGCCT CTGTTAGTCA GAACATTGAT 
CAAACAAACA CTAGTGAGAT TTCTGATAAG CTACAAGCGA CTGAAAATTT TAAACAACAG 
TCTCTAGACA CTTCAAATTC TAGCACAGCT TCAGATGCAC CAATAATTTC TGATGTAACT 
GAAGAGAAAT CAAGTTCTGT CTTGCAGGAT GAACAGTTGA ATGTTGTTAA CTGCCAAAAT 
GACATTCCAG AACATTCAAT TCATGATATC AAGGAGCTCG AACGAGTTAC AAAGGAATTA 
CAAGCAGCTC AACTCGAGCT AAATGTGATG AAGGCTCAGA ATTCAGAGCT AGCTCTACAG 
AGAGACATGA TAAAAGATAA GCTACTGAAT GTTCAGCAAG AGAATCAAGA GTTGAAATCA 
ACTCTGAAAC ACTTGTGTGA AGACACTGGT GCTAAACAAG ATGGTGCCTT AAATTTAGCG 
AACTCTAAAA ACAAATCGTT GTTGAGTTCA AATGAAGGAT TGCAAAGCAT CTCCCCATTA 
GGGAAAGAGA AATTGGTCGT TCAGCATGGA AACTCTGAAG CGGAGGTTCA GTCTCTTCAG 
GAGCAGATCC AAAAGTTGCA GGACCAAATC CGGGACCTCT CTGAACAAAA CAGGACTCAG 
GCTGAACAGC TGGAGCTCTG GAAGCTGTCA ACTGTGGAAG AAACAGCGGG CAACAACTCA 
CCCTCTATTG TGCTAAAAGA GTTTGAGCTG TTCCTTCCCT GCGGTTCTAG AAAACTGCAC 
ACACACTCCC AAACCAGGAC CATGATGCAC CAGTGCGATG TCAGAGATGT GACTTATCAC 
TCTGGATTAG ATGACTCAAA TCTGACCAAC AGTGACGAGA AAGTGTCGTC TGAAACCCAA 
ATAACAATTG ATGTGGAAAA TTCGCAAGGA CCTGCAGAGG ACATTATGGC TAGAAATAAG 
AAGCTGCTAA CCAAGGATAA TGCTCAACAC AAGTGTGAGA ACAGCTCTGC TAACCACTAC 
TGCCATAAAA GTCCTCCTGT CTGCATCTCA GATATAATGA CTAGTAACAC TCGTGACAAG 
CTGTCACCGC TACTGACCAA ACCAACAGAT TACAGTCATT CAGAGTCTCA GCCGGTTGCC 
TCATTGTCTC CTGAGGGTCA GCCCAAAGTG ACAGATGCCA GTGCTAACAG CCAGGAAGAT 
GCCTTAATCA GTGTTTCCAT CTCAACCAAA TATCAGGCCG AGGTAATCAA CACAAATGCA 
ATCACTGAGT CCTATGGGAA CGCTGATTTC CACAATTCTG AAACTATGGG AACTGTTGCT 
TTGGATAAAC ATCGTGGAGA TGCAACAGAT AAACAGAACA AACATTACAC TGCTGTGGAA 
AACACAATCA GAAACGAATC AGCTCAGGGT CAAAAAGCAG AGGTCAGTGG AGTTTATAAG 
AGAACAGGGG TCATAAGTGT GTCCACGCAG ACGGAGGAGA GTCGAGAGAT GCATTTAGCA 
GTCAAACAGC CGCCCGTCCA CGCCTGCACT CAGACAGATG GAGAACAGCT GCAGACGGAG 
CAAGATGAAG GTGATAAAGC GTCTGTAGAC TCACCGGCCC TCTCCCCGAC CCCACACACT 
GTTACAAACC AACTGCTGCT ATCCAGCGTG TTCCCTATGA GTGACCCAGC ACACCTCGCT 
GAACGCATCC GGCAAAACCG CAACCGCATG TCTGCTGCTT ATGATGAGAC CGAATATGAA 
CCATACGGCC TGCCAGAGGT TGTAATGAAA GGATTTGCCG ACATTCCCAG TGGGCCAGCC 
TGTCCGTATG TCCTGCGCAG AGGGCTTCTG GGAACTGACG CAATGCCCCT CCCACGCAGA 
GGAGAAGCCA CGCCCCTGGA GGAAGATGAC GACATAGAGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-115E12.6 ( XM_021481113.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336788.1 Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X2, mRNA.

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CloneID	ODa19388
Clone ID Related Accession (Same CDS sequence)	XM_005157165.3 , XM_021481113.1
Accession Version	XM_021481113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centromere protein F-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CTGCAGTTTG GAGACGTCCC CAAGCCCTCA 
GCAGGCGGGG CCTCCTCCCC GTTTCCACAG CAACCATATA AATCCCCGCC CCTCCGGCGC 
CACGTCCGCC AATCAGAGCC ATCCACCCCC TCCTCTGTGT TTCCATGGGA ACGTGATGTC 
TTGTGGTCCA CTCCCAAAGG ACGACCGACT TCCTTGGTTT CATCCAGTGA GGTCATGATC 
AAGAGCAGTA ATTGTGGGAT GGAGGACGCC CTGAGGAACG AGATCGATGA GCTGCGTGTG 
CGTGTTTCAG ATCTACAGCG CGAGGCACAG CTGGAGACTG AGCGCATGAA GGATGTGGAG 
TCTCGTCTGG CTCAGGCTAA CAGAGAGATC AGCAGCAAAG AGCAGAGCCT GACGCGCACG 
CAAGAGCAGC TGACCCGCGC ACAGACCCGC AGCACACAGG AAACTGACAG AGCCCAGACA 
GCTGAGCAGA AAGTCAAGCA CCTTCAGGAG GAGCTGAAAT GTCAAAGGCA GAATGCTGAA 
AGCAGTCGCT GCAATGCAGA GCAGCGCAGG AAAGACATGG AGAGAGAACA TCAACGGGAG 
CTGGTGGAGC AGCAGAGAGA GAGGCAGGCT CTAGAGAAAC AGCATCAGCA GGAGAACAAC 
AGACTAAACC AGGAGATCCA GCAGGCCAGA ACTCTTCACA ACACACTGCA GGCTCAGCAT 
GACAAGGTGT GTTTGCAGAA GCAGAGTTTG GAGCGAGATC TGGAGGATGT GAAAGGGAAA 
CTGAAGAACA CTGAGGCTGA CCTGAAAGAA AGTCAGAAGA GAGAGGCACA AACTGAGGCA 
AAACTCACGG AGGCGCTGAG GGAGTGTGAG AGTCTCACTG TTTCTCTGGG GCAGATGAAA 
AAACAGGAGA AAGTGCTACA GGAGGAAGTG AAGCGCTTGA CTGAAGAGCT TGCTGAGGCG 
CTCAAGCTTA TTAAAGAACT GCAAGCTCAG CTGGCCGCTC CTCCTCCACC TGTTGCTGCC 
CCTAACTTTA GCTCAGCTGG AGACTCCTTC TCTCCCTGTG TTTCCCTCCA TCACGACCGC 
TCTTCCCCTT TCAACAACTC CTCTCAGAGA AAAAGAGCCC CTAATACAGG GAGAACAAGG 
GAAGAGGAGA GAATGAAGTA TCCCTCCGGT AGAGAGCCTG GAGAGGGCAT CGACTCAGAG 
CACATTGAGA AATTTGAGTC CGAGGAGAAA TCCCAAAAGC GTAAAGGAGT CCAAACACTT 
CCAATTGGCT CTCTGAAACT GACTGAGTTT GATTTAGACA CCTCAGTGAT GGAACAAGAC 
ACGGGTATTG AAGATGTGGA TACGGACTCC TTCACGTCCG ACTCAACCAG GGGCACGATG 
CCTAAAAGTG AATCAGACAT CAGTCGCAGC CTAAAGTTAG ACAATACATC AGGTAAAACA 
GAAAAATATG ACTCTACAAC TCTGAAAGAT CTGAAGAAGC AGAATGCAGA GCTTCAAGAT 
GAGCTTAGAG ATGTGAAATA TGACCTTCAG AAGCGACTTG AGGATCTTGA AACCCAGAGG 
AGAGCAGAAA CTGAGGCCAG GACCAAATTG AAGCAATTGA GTCGCAAACA CTCAACCCAG 
ACAGAGCAGC AACGTGCCAA AGCTCTGGAG CTCAAGGATA GCCTGGTGAA ATTGGAAACT 
CAGCTGGAAC AAGAGAAGAA GGAGAGCTCA AAATTAAAGG AAACACTTCA TGCTCTACAA 
AGTGAAGCTG AGAACAGGAA GGTGAATGAC CAGGAAGAAG ATTCACAGCT GAAGAAGGCC 
TTGGCTGAAA TGGAGCAAAA GGAGACCATC ATGGAAGAAG AAATGATGGG AATCAAAAAG 
GAACTGCAAG ACCTTCAACT AAAGCTTGCA CAGGAACGGG AGGAGAGGGA GCAGGAGAGA 
TTGGAGGAAA GGAAGCTGAT CAGAAGAGAG GAAGGACTAA AGATTGCACA GCTTCAGGAA 
GAGCTGGACA TTCTTCGAAA GTCTACGAGT TTGGAGGTGA AGATATCAAA AGACAATCTT 
CCCCTCACGT ATTTACATCT AGAACACCAC CAAAACACTG ATCAGAAAGC TGATCTTACC 
GAAAACAAAG ACTTGATTCC TTCTCCAGGA GTTCAAGAGT TTTCTGTGAA TCTTCAGAAC 
ACAATAGTCC GCAATGAAGC TAAAACAATT GAGCTGATTA TGGACCTTGA AGCACAAACT 
AAACCGAAGA CCTCTCCTTC AGATATGAAT AGGGCTCGAG CATTGAGTAC AGAAGCTTCA 
GATTTGGACA ACTCTACGGT TTTAGTTTTG GAGGTGGAAC GCTTGAGAGC TGCGCGAGAC 
AGAGAGTCTG AGAAGGCAAA AGTATCTCAA AGAAAGCTGG AGGAGCTACA GAAGCAAGTG 
ACCACTCAGA CCAAGCAACT CACCCAAGCT TTCGAAAGCC AGAGCAAGCA CATTGACAAC 
TTGTTAAGCG AGTTAGAGGA TAAAGAGTGT GCTCTCCAGA AGCAGGCAGA GGAACTGCAA 
AAATGCCAGG AGAAAATCTG TTTTCTTGAA GAAAAACAAA CTAGGACGAA TGATCTCTCT 
GTACCTCCTG AACTATCTAC AGAGATCTCA AGAGAACTGA GCTGTGATTC AGTCTTCAGC 
AACACGAGTA TAAGTGACCA TGAAATGTTT AGTGATGATA CACCACCGGT TTTGAAGGAG 
AAGGTCTCAG AACTTACTGT ACAGCTTCCT GTTGAAGCCT CTGTTAGTCA GAACATTGAT 
CAAACAAACA CTAGTGAGAT TTCTGATAAG CTACAAGCGA CTGAAAATTT TAAACAACAG 
TCTCTAGACA CTTCAAATTC TAGCACAGCT TCAGATGCAC CAATAATTTC TGATGTAACT 
GAAGAGAAAT CAAGTTCTGT CTTGCAGGAT GAACAGTTGA ATGTTGTTAA CTGCCAAAAT 
GACATTCCAG AACATTCAAT TCATGATATC AAGGAGCTCG AACGAGTTAC AAAGGAATTA 
CAAGCAGCTC AACTCGAGCT AAATGTGATG AAGGCTCAGA ATTCAGAGCT AGCTCTACAG 
AGAGACATGA TAAAAGATAA GCTACTGAAT GTTCAGCAAG AGAATCAAGA GTTGAAATCA 
ACTCTGAAAC ACTTGTGTGA AGACACTGGT GCTAAACAAG ATGGTGCCTT AAATTTAGCG 
AACTCTAAAA ACAAATCGTT GTTGAGTTCA AATGAAGGAT TGCAAAGCAT CTCCCCATTA 
GGGAAAGAGA AATTGGTCGT TCAGCATGGA AACTCTGAAG CGGAGGTTCA GTCTCTTCAG 
GAGCAGATCC AAAAGTTGCA GGACCAAATC CGGGACCTCT CTGAACAAAA CAGGACTCAG 
GCTGAACAGC TGGAGCTCTG GAAGCTGTCA ACTGTGGAAG AAACAGCGGG CAACAACTCA 
CCCTCTATTG TGCTAAAAGA GTTTGAGCTG TTCCTTCCCT GCGGTTCTAG AAAACTGCAC 
ACACACTCCC AAACCAGGAC CATGATGCAC CAGTGCGATG TCAGAGATGT GACTTATCAC 
TCTGGATTAG ATGACTCAAA TCTGACCAAC AGTGACGAGA AAGTGTCGTC TGAAACCCAA 
ATAACAATTG ATGTGGAAAA TTCGCAAGGA CCTGCAGAGG ACATTATGGC TAGAAATAAG 
AAGCTGCTAA CCAAGGATAA TGCTCAACAC AAGTGTGAGA ACAGCTCTGC TAACCACTAC 
TGCCATAAAA GTCCTCCTGT CTGCATCTCA GATATAATGA CTAGTAACAC TCGTGACAAG 
CTGTCACCGC TACTGACCAA ACCAACAGAT TACAGTCATT CAGAGTCTCA GCCGGTTGCC 
TCATTGTCTC CTGAGGGTCA GCCCAAAGTG ACAGATGCCA GTGCTAACAG CCAGGAAGAT 
GCCTTAATCA GTGTTTCCAT CTCAACCAAA TATCAGGCCG AGGTAATCAA CACAAATGCA 
ATCACTGAGT CCTATGGGAA CGCTGATTTC CACAATTCTG AAACTATGGG AACTGTTGCT 
TTGGATAAAC ATCGTGGAGA TGCAACAGAT AAACAGAACA AACATTACAC TGCTGTGGAA 
AACACAATCA GAAACGAATC AGCTCAGGGT CAAAAAGCAG AGGTCAGTGG AGTTTATAAG 
AGAACAGGGG TCATAAGTGT GTCCACGCAG ACGGAGGAGA GTCGAGAGAT GCATTTAGCA 
GTCAAACAGC CGCCCGTCCA CGCCTGCACT CAGACAGATG GAGAACAGCT GCAGACGGAG 
CAAGATGAAG GTGATAAAGC GTCTGTAGAC TCACCGGCCC TCTCCCCGAC CCCACACACT 
GTTACAAACC AACTGCTGCT ATCCAGCGTG TTCCCTATGA GTGACCCAGC ACACCTCGCT 
GAACGCATCC GGCAAAACCG CAACCGCATG TCTGCTGCTT ATGATGAGAC CGAATATGAA 
CCATACGGCC TGCCAGAGGT TGTAATGAAA GGATTTGCCG ACATTCCCAG TGGGCCAGCC 
TGTCCGTATG TCCTGCGCAG AGGGCTTCTG GGAACTGACG CAATGCCCCT CCCACGCAGA 
GGAGAAGCCA CGCCCCTGGA GGAAGATGAC GACATAGAGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336788.1
CDS	284..5368
Translation	MSWAGDDWTVGLTGHVLQKVKQLLAQNEKLNKEKQQRQLQLDNSEVALHKQKQKHEEVRVELAAVQRELGGVREAAQAEVRTRERLSHDLQVKTGQVHSLEGQLESAKKLTQSLTQEIKRLEAELEKLQKGNGSGESTLFSTPCWNTSSPWDNNGGFRAESESKAQHARQLQFGDVPKPSAGGASSPFPQQPYKSPPLRRHVRQSEPSTPSSVFPWERDVLWSTPKGRPTSLVSSSEVMIKSSNCGMEDALRNEIDELRVRVSDLQREAQLETERMKDVESRLAQANREISSKEQSLTRTQEQLTRAQTRSTQETDRAQTAEQKVKHLQEELKCQRQNAESSRCNAEQRRKDMEREHQRELVEQQRERQALEKQHQQENNRLNQEIQQARTLHNTLQAQHDKVCLQKQSLERDLEDVKGKLKNTEADLKESQKREAQTEAKLTEALRECESLTVSLGQMKKQEKVLQEEVKRLTEELAEALKLIKELQAQLAAPPPPVAAPNFSSAGDSFSPCVSLHHDRSSPFNNSSQRKRAPNTGRTREEERMKYPSGREPGEGIDSEHIEKFESEEKSQKRKGVQTLPIGSLKLTEFDLDTSVMEQDTGIEDVDTDSFTSDSTRGTMPKSESDISRSLKLDNTSGKTEKYDSTTLKDLKKQNAELQDELRDVKYDLQKRLEDLETQRRAETEARTKLKQLSRKHSTQTEQQRAKALELKDSLVKLETQLEQEKKESSKLKETLHALQSEAENRKVNDQEEDSQLKKALAEMEQKETIMEEEMMGIKKELQDLQLKLAQEREEREQERLEERKLIRREEGLKIAQLQEELDILRKSTSLEVKISKDNLPLTYLHLEHHQNTDQKADLTENKDLIPSPGVQEFSVNLQNTIVRNEAKTIELIMDLEAQTKPKTSPSDMNRARALSTEASDLDNSTVLVLEVERLRAARDRESEKAKVSQRKLEELQKQVTTQTKQLTQAFESQSKHIDNLLSELEDKECALQKQAEELQKCQEKICFLEEKQTRTNDLSVPPELSTEISRELSCDSVFSNTSISDHEMFSDDTPPVLKEKVSELTVQLPVEASVSQNIDQTNTSEISDKLQATENFKQQSLDTSNSSTASDAPIISDVTEEKSSSVLQDEQLNVVNCQNDIPEHSIHDIKELERVTKELQAAQLELNVMKAQNSELALQRDMIKDKLLNVQQENQELKSTLKHLCEDTGAKQDGALNLANSKNKSLLSSNEGLQSISPLGKEKLVVQHGNSEAEVQSLQEQIQKLQDQIRDLSEQNRTQAEQLELWKLSTVEETAGNNSPSIVLKEFELFLPCGSRKLHTHSQTRTMMHQCDVRDVTYHSGLDDSNLTNSDEKVSSETQITIDVENSQGPAEDIMARNKKLLTKDNAQHKCENSSANHYCHKSPPVCISDIMTSNTRDKLSPLLTKPTDYSHSESQPVASLSPEGQPKVTDASANSQEDALISVSISTKYQAEVINTNAITESYGNADFHNSETMGTVALDKHRGDATDKQNKHYTAVENTIRNESAQGQKAEVSGVYKRTGVISVSTQTEESREMHLAVKQPPVHACTQTDGEQLQTEQDEGDKASVDSPALSPTPHTVTNQLLLSSVFPMSDPAHLAERIRQNRNRMSAAYDETEYEPYGLPEVVMKGFADIPSGPACPYVLRRGLLGTDAMPLPRRGEATPLEEDDDIEP

Target ORF information:

RefSeq Version	XM_021481113.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-115e12.6 (si:dkeyp-115e12.6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481113.1

ORF Insert Sequence:

ATGAGCTGGG CTGGAGATGA CTGGACTGTG GGTCTGACGG GTCACGTCCT GCAGAAGGTT 
AAACAGCTCC TGGCTCAGAA CGAGAAGCTG AACAAAGAGA AACAGCAGAG GCAGCTGCAG 
CTGGATAACT CTGAGGTCGC TCTGCACAAA CAGAAGCAGA AGCATGAGGA GGTGCGTGTG 
GAGCTGGCCG CAGTTCAGAG GGAGCTGGGA GGAGTACGAG AGGCGGCACA GGCCGAAGTG 
CGGACCAGAG AACGTCTGTC TCATGACCTG CAGGTCAAAA CGGGTCAAGT GCACTCGCTG 
GAGGGACAGC TGGAGTCTGC CAAAAAGCTG ACACAAAGCC TCACGCAGGA GATCAAACGG 
CTTGAGGCAG AGCTGGAAAA GCTGCAGAAG GGGAACGGCT CTGGGGAATC CACGTTGTTT 
TCCACACCTT GTTGGAACAC GAGCTCTCCC TGGGACAACA ACGGTGGTTT CAGAGCGGAG 
AGCGAAAGCA AAGCACAGCA TGCCAGACAG CTGCAGTTTG GAGACGTCCC CAAGCCCTCA 
GCAGGCGGGG CCTCCTCCCC GTTTCCACAG CAACCATATA AATCCCCGCC CCTCCGGCGC 
CACGTCCGCC AATCAGAGCC ATCCACCCCC TCCTCTGTGT TTCCATGGGA ACGTGATGTC 
TTGTGGTCCA CTCCCAAAGG ACGACCGACT TCCTTGGTTT CATCCAGTGA GGTCATGATC 
AAGAGCAGTA ATTGTGGGAT GGAGGACGCC CTGAGGAACG AGATCGATGA GCTGCGTGTG 
CGTGTTTCAG ATCTACAGCG CGAGGCACAG CTGGAGACTG AGCGCATGAA GGATGTGGAG 
TCTCGTCTGG CTCAGGCTAA CAGAGAGATC AGCAGCAAAG AGCAGAGCCT GACGCGCACG 
CAAGAGCAGC TGACCCGCGC ACAGACCCGC AGCACACAGG AAACTGACAG AGCCCAGACA 
GCTGAGCAGA AAGTCAAGCA CCTTCAGGAG GAGCTGAAAT GTCAAAGGCA GAATGCTGAA 
AGCAGTCGCT GCAATGCAGA GCAGCGCAGG AAAGACATGG AGAGAGAACA TCAACGGGAG 
CTGGTGGAGC AGCAGAGAGA GAGGCAGGCT CTAGAGAAAC AGCATCAGCA GGAGAACAAC 
AGACTAAACC AGGAGATCCA GCAGGCCAGA ACTCTTCACA ACACACTGCA GGCTCAGCAT 
GACAAGGTGT GTTTGCAGAA GCAGAGTTTG GAGCGAGATC TGGAGGATGT GAAAGGGAAA 
CTGAAGAACA CTGAGGCTGA CCTGAAAGAA AGTCAGAAGA GAGAGGCACA AACTGAGGCA 
AAACTCACGG AGGCGCTGAG GGAGTGTGAG AGTCTCACTG TTTCTCTGGG GCAGATGAAA 
AAACAGGAGA AAGTGCTACA GGAGGAAGTG AAGCGCTTGA CTGAAGAGCT TGCTGAGGCG 
CTCAAGCTTA TTAAAGAACT GCAAGCTCAG CTGGCCGCTC CTCCTCCACC TGTTGCTGCC 
CCTAACTTTA GCTCAGCTGG AGACTCCTTC TCTCCCTGTG TTTCCCTCCA TCACGACCGC 
TCTTCCCCTT TCAACAACTC CTCTCAGAGA AAAAGAGCCC CTAATACAGG GAGAACAAGG 
GAAGAGGAGA GAATGAAGTA TCCCTCCGGT AGAGAGCCTG GAGAGGGCAT CGACTCAGAG 
CACATTGAGA AATTTGAGTC CGAGGAGAAA TCCCAAAAGC GTAAAGGAGT CCAAACACTT 
CCAATTGGCT CTCTGAAACT GACTGAGTTT GATTTAGACA CCTCAGTGAT GGAACAAGAC 
ACGGGTATTG AAGATGTGGA TACGGACTCC TTCACGTCCG ACTCAACCAG GGGCACGATG 
CCTAAAAGTG AATCAGACAT CAGTCGCAGC CTAAAGTTAG ACAATACATC AGGTAAAACA 
GAAAAATATG ACTCTACAAC TCTGAAAGAT CTGAAGAAGC AGAATGCAGA GCTTCAAGAT 
GAGCTTAGAG ATGTGAAATA TGACCTTCAG AAGCGACTTG AGGATCTTGA AACCCAGAGG 
AGAGCAGAAA CTGAGGCCAG GACCAAATTG AAGCAATTGA GTCGCAAACA CTCAACCCAG 
ACAGAGCAGC AACGTGCCAA AGCTCTGGAG CTCAAGGATA GCCTGGTGAA ATTGGAAACT 
CAGCTGGAAC AAGAGAAGAA GGAGAGCTCA AAATTAAAGG AAACACTTCA TGCTCTACAA 
AGTGAAGCTG AGAACAGGAA GGTGAATGAC CAGGAAGAAG ATTCACAGCT GAAGAAGGCC 
TTGGCTGAAA TGGAGCAAAA GGAGACCATC ATGGAAGAAG AAATGATGGG AATCAAAAAG 
GAACTGCAAG ACCTTCAACT AAAGCTTGCA CAGGAACGGG AGGAGAGGGA GCAGGAGAGA 
TTGGAGGAAA GGAAGCTGAT CAGAAGAGAG GAAGGACTAA AGATTGCACA GCTTCAGGAA 
GAGCTGGACA TTCTTCGAAA GTCTACGAGT TTGGAGGTGA AGATATCAAA AGACAATCTT 
CCCCTCACGT ATTTACATCT AGAACACCAC CAAAACACTG ATCAGAAAGC TGATCTTACC 
GAAAACAAAG ACTTGATTCC TTCTCCAGGA GTTCAAGAGT TTTCTGTGAA TCTTCAGAAC 
ACAATAGTCC GCAATGAAGC TAAAACAATT GAGCTGATTA TGGACCTTGA AGCACAAACT 
AAACCGAAGA CCTCTCCTTC AGATATGAAT AGGGCTCGAG CATTGAGTAC AGAAGCTTCA 
GATTTGGACA ACTCTACGGT TTTAGTTTTG GAGGTGGAAC GCTTGAGAGC TGCGCGAGAC 
AGAGAGTCTG AGAAGGCAAA AGTATCTCAA AGAAAGCTGG AGGAGCTACA GAAGCAAGTG 
ACCACTCAGA CCAAGCAACT CACCCAAGCT TTCGAAAGCC AGAGCAAGCA CATTGACAAC 
TTGTTAAGCG AGTTAGAGGA TAAAGAGTGT GCTCTCCAGA AGCAGGCAGA GGAACTGCAA 
AAATGCCAGG AGAAAATCTG TTTTCTTGAA GAAAAACAAA CTAGGACGAA TGATCTCTCT 
GTACCTCCTG AACTATCTAC AGAGATCTCA AGAGAACTGA GCTGTGATTC AGTCTTCAGC 
AACACGAGTA TAAGTGACCA TGAAATGTTT AGTGATGATA CACCACCGGT TTTGAAGGAG 
AAGGTCTCAG AACTTACTGT ACAGCTTCCT GTTGAAGCCT CTGTTAGTCA GAACATTGAT 
CAAACAAACA CTAGTGAGAT TTCTGATAAG CTACAAGCGA CTGAAAATTT TAAACAACAG 
TCTCTAGACA CTTCAAATTC TAGCACAGCT TCAGATGCAC CAATAATTTC TGATGTAACT 
GAAGAGAAAT CAAGTTCTGT CTTGCAGGAT GAACAGTTGA ATGTTGTTAA CTGCCAAAAT 
GACATTCCAG AACATTCAAT TCATGATATC AAGGAGCTCG AACGAGTTAC AAAGGAATTA 
CAAGCAGCTC AACTCGAGCT AAATGTGATG AAGGCTCAGA ATTCAGAGCT AGCTCTACAG 
AGAGACATGA TAAAAGATAA GCTACTGAAT GTTCAGCAAG AGAATCAAGA GTTGAAATCA 
ACTCTGAAAC ACTTGTGTGA AGACACTGGT GCTAAACAAG ATGGTGCCTT AAATTTAGCG 
AACTCTAAAA ACAAATCGTT GTTGAGTTCA AATGAAGGAT TGCAAAGCAT CTCCCCATTA 
GGGAAAGAGA AATTGGTCGT TCAGCATGGA AACTCTGAAG CGGAGGTTCA GTCTCTTCAG 
GAGCAGATCC AAAAGTTGCA GGACCAAATC CGGGACCTCT CTGAACAAAA CAGGACTCAG 
GCTGAACAGC TGGAGCTCTG GAAGCTGTCA ACTGTGGAAG AAACAGCGGG CAACAACTCA 
CCCTCTATTG TGCTAAAAGA GTTTGAGCTG TTCCTTCCCT GCGGTTCTAG AAAACTGCAC 
ACACACTCCC AAACCAGGAC CATGATGCAC CAGTGCGATG TCAGAGATGT GACTTATCAC 
TCTGGATTAG ATGACTCAAA TCTGACCAAC AGTGACGAGA AAGTGTCGTC TGAAACCCAA 
ATAACAATTG ATGTGGAAAA TTCGCAAGGA CCTGCAGAGG ACATTATGGC TAGAAATAAG 
AAGCTGCTAA CCAAGGATAA TGCTCAACAC AAGTGTGAGA ACAGCTCTGC TAACCACTAC 
TGCCATAAAA GTCCTCCTGT CTGCATCTCA GATATAATGA CTAGTAACAC TCGTGACAAG 
CTGTCACCGC TACTGACCAA ACCAACAGAT TACAGTCATT CAGAGTCTCA GCCGGTTGCC 
TCATTGTCTC CTGAGGGTCA GCCCAAAGTG ACAGATGCCA GTGCTAACAG CCAGGAAGAT 
GCCTTAATCA GTGTTTCCAT CTCAACCAAA TATCAGGCCG AGGTAATCAA CACAAATGCA 
ATCACTGAGT CCTATGGGAA CGCTGATTTC CACAATTCTG AAACTATGGG AACTGTTGCT 
TTGGATAAAC ATCGTGGAGA TGCAACAGAT AAACAGAACA AACATTACAC TGCTGTGGAA 
AACACAATCA GAAACGAATC AGCTCAGGGT CAAAAAGCAG AGGTCAGTGG AGTTTATAAG 
AGAACAGGGG TCATAAGTGT GTCCACGCAG ACGGAGGAGA GTCGAGAGAT GCATTTAGCA 
GTCAAACAGC CGCCCGTCCA CGCCTGCACT CAGACAGATG GAGAACAGCT GCAGACGGAG 
CAAGATGAAG GTGATAAAGC GTCTGTAGAC TCACCGGCCC TCTCCCCGAC CCCACACACT 
GTTACAAACC AACTGCTGCT ATCCAGCGTG TTCCCTATGA GTGACCCAGC ACACCTCGCT 
GAACGCATCC GGCAAAACCG CAACCGCATG TCTGCTGCTT ATGATGAGAC CGAATATGAA 
CCATACGGCC TGCCAGAGGT TGTAATGAAA GGATTTGCCG ACATTCCCAG TGGGCCAGCC 
TGTCCGTATG TCCTGCGCAG AGGGCTTCTG GGAACTGACG CAATGCCCCT CCCACGCAGA 
GGAGAAGCCA CGCCCCTGGA GGAAGATGAC GACATAGAGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SI:DKEYP-115E12.6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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