syd-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	syd-2
Entrez Gene ID	181255
Full Name	Liprin-alpha
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_077367.8	NP_509768.1	Liprin-alpha
NM_077367.6	NP_509768.1	Liprin-alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPFIA2	NP_003616.2
Canis lupus familiaris (dog)	PPFIA2	XP_003432068.2
Xenopus tropicalis (tropical clawed frog)	ppfia2	XP_002931667.2
Rattus norvegicus (Norway rat)	Ppfia2	NP_001102215.1
Danio rerio (zebrafish)	ppfia2	NP_001038576.1
Caenorhabditis elegans (roundworm)	syd-2	NP_509768.1
Mus musculus (house mouse)	Ppfia2	NP_001192270.1
	PPFIA2	XP_001088353.2
Gallus gallus (chicken)	PPFIA2	XP_416120.4
Pan troglodytes (chimpanzee)	PPFIA2	XP_509243.2
Drosophila melanogaster (fruit fly)	Liprin-alpha	NP_001260155.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001469	XP_321656.5

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data reveal a new and unprecedented role for 2 synapse assembly proteins, SAD-1 and SYD-2, in mediating UNC-16's organelle transport regulatory function.
Title: UNC-16 (JIP3) Acts Through Synapse-Assembly Proteins to Inhibit the Active Transport of Cell Soma Organelles to Caenorhabditis elegans Motor Neuron Axons.

A main role of SYD-2/Liprin-alpha in synaptogenesis is to regulate the polymerization of DPs.
Title: Liprin-?/SYD-2 determines the size of dense projections in presynaptic active zones in C. elegans.

N-terminal CC domains are responsible for the synaptogenic activity of SYD-2.
Title: Intramolecular regulation of presynaptic scaffold protein SYD-2/liprin-?.

tudy shows that SYD-2 promotes bi-directional mobility of DCVs and identifies SYD-2 as a novel regulator of DCV trafficking and polarized distribution
Title: The scaffolding protein SYD-2/Liprin-? regulates the mobility and polarized distribution of dense-core vesicles in C. elegans motor neurons.

we propose that the dense projection ensures proper coupling of primed vesicles with calcium signaling by retaining them at the active zone via UNC-10 and SYD-2 dependent mechanisms
Title: The presynaptic dense projection of the Caenorhabditis elegans cholinergic neuromuscular junction localizes synaptic vesicles at the active zone through SYD-2/liprin and UNC-10/RIM-dependent interactions.

The following syd-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the syd-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa350140	NM_077367.8 Latest version!	Caenorhabditis elegans Liprin-alpha (syd-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa350140	NM_077367.6	Caenorhabditis elegans Liprin-alpha (syd-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

syd-2 ( NM_077367.8 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509768.1 Caenorhabditis elegans Liprin-alpha (syd-2), mRNA.

Price & Availability↑

CloneID	OCa350140
Clone ID Related Accession (Same CDS sequence)	NM_077367.8 , NM_077367.6
Accession Version	NM_077367.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Liprin-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_077367.7.

ATGAGCTACA GCAATGGAAA CATAAATTGT GATATAATGC CGACAATCTC GGAAGATGGA 
GTGGACAACG GCGGTCCCAT TGATGAACCA TCGGACCGAG ACAACATTGA GCAGCTGATG 
ATGAACATGT TGGAAGACCG GGACAAATTA CAAGAACAGT TGGAAAATTA TAAGGTGCAA 
CTGGAAAATG CAGGGCTCCG AACGAAAGAA GTTGAAAAAG AACGAGATAT GATGAAGAGA 
CAGTTCGAAG TTCATACGCA AAATTTACCG CAGGAACTTC AAACAATGAC GCGGGAATTA 
TGCCTATTAA AAGAGCAGTT ATTAGAAAAA GATGAAGAGA TTGTCGAGCT CAAAGCAGAA 
CGGAACAATA CTCGACTTCT TCTAGAGCAC CTAGAATGTT TAGTTTCCCG TCATGAAAGA 
TCACTCCGAA TGACGGTCAT GAAGAGACAA GCTCAAAACC ACGCTGGCGT ATCTTCTGAA 
GTTGAAGTTC TAAAAGCTCT TAAGAGTTTA TTCGAGCATC ACAAGGCATT GGATGAAAAA 
GTTCGTGAAC GTCTTCGAGT AGCAATGGAG CGTGTGGCGA CACTTGAAGA AGAGCTGAGC 
ACAAAAGGAG ATGAGAATTC GTCGCTGAAA GCGCGTATTG CCACTTACGC AGCAGAAGCG 
GAAGAAGCAA TGGCGTCAAA TGCACCCATC AATGGTTCAA TTAGCAGTGA AAGTGCAAAC 
AGACTTATCG AAATGCAAGA GGCTTTAGAA AGAATGAAAA CAGAATTAGC CAACTCACTA 
AAACAATCAA CTGAGATCAC AACAAGAAAT GCCGAACTCG AGGATCAATT GACAGAAGAT 
GCTCGTGAAA AACATGCTGC ACAGGAATCA ATCGTTCGTT TGAAGAATCA AATTTGCGAG 
CTTGATGCAC AACGCACCGA TCAAGAAACT CGGATAACAA CGTTTGAGTC GCGTTTCCTG 
ACCGCGCAAA GAGAGTCAAC GTGTATCCGG GACCTCAATG ACAAATTGGA GCATCAGCTT 
GCCAATAAAG ATGCGGCTGT CAGGTTGAAT GAGGAAAAAG TTCATTCACT TCAAGAAAGA 
TTGGAGTTGG CAGAGAAGCA ACTTGCACAA AGTCTCAAAA AAGCTGAATC CCTTCCGTCT 
GTAGAAGCCG AATTACAACA AAGAATGGAA GCACTTACAG CAGCTGAACA GAAGAGTGTT 
TCTGCTGAAG AGCGCATCCA AAGGTTGGAC CGAAACATTC AAGAGTTGTC TGCAGAGTTG 
GAAAGGGCTG TGCAAAGAGA ACGCATGAAC GAAGAGCACA GCCAACGGTT AAGTAGCACT 
GTGGACAAGC TTTTATCGGA ATCTAATGAT CGCCTTCAAC TGCATCTCAA AGAACGAATG 
CAAGCCCTCG ATGACAAAAA TCGGTTAACA CAACAGCTTG ATGGAACAAA GAAAATTTAT 
GATCAAGCGG AACGAATCAA AGATCGTCTT CAGCGGGATA ATGAATCTCT ACGGCAAGAG 
ATTGAAGCTC TTAGGCAACA ATTGTACAAT GCAAGAACTG CGCAATTCCA GTCAAGAATG 
CATGCAATTC CGTTTACACA TGCTCAAAAC ATTGTGCAGC AACAACCACA AGCTTCGATT 
GCTCAGCAAT CTGCATACCA AATGTACAAA CAACAACCAG CTCAACAGTA TCAAACTGTA 
GGAATGAGAA GACCAAATAA AGGAAGGATT TCAGCACTCC AGGACGATCC GAACAAAGTT 
CAAACCCTCA ACGAGCAAGA ATGGGATCGA CTTCAACAAG CGCATGTTCT TGCCAACGTC 
CAGCAAGCAT TTTCCTCATC ACCATCACTG GCCGACGTCG GTCAATCGAC TCTTCCACGT 
CCTAACACTG CTGTCCAACA CCAGCAAGAT GATATGATGA ACAGTGGAAT GGGAATGCCA 
AGTGGAATGC AGGGAGGAAT GCAAGGTGGA ATGGGTGGTG GTCAAGATGC TCAGATGTTG 
GCATCAATGC TACAAGATCG CTTAGATGCA ATAAATACTG AAATAAGATT GATTCAACAA 
GAGAAACATC ACGCAGAACG GGTGGCTGAA CAGTTAGAAC GATCAAGTCG GGAATTCTAT 
GATGATCAGG GAATATCTAC GAGAAGTTCA CCAAGAGCAA GTCCCCAATT AGATAATATG 
CGACAACATA AGTATAACAC TCTTCCTGCA AATGTATCCG GTGACCGACG GTATGATATT 
TACGGCAATC CGCAGTTTGT TGACGACCGC ATGGTTAGAG ATTTGGACTA CGAGCCACGA 
CGAGGGTACA ACCAATTTGA CGAGATGCAA TACGAAAGAG ATCGAATGAG TCCAGCTTCG 
TCAGTCGCTT CCTCGACAGA TGGCGTTCTT GGCGGAAAGA AGAAAAGATC GAATAGCTCA 
AGTGGATTGA AAACGCTTGG AAGGTTTTTC AACAAAAAGA AAAATTCATC GAGTGATTTA 
TTCAAACGAA ATGGAGATTA TTCGGATGGC GAGCAGTCGG GCACAGAAGG AAACCAAAAA 
GCTGATTACG ACAGGAGGAA AAAGAAGAAG CATGAACTAT TAGAGGAAGC CATGAAAGCA 
CGAACACCGT TTGCTCTTTG GAATGGTCCA ACTGTTGTTG CCTGGCTGGA GCTGTGGGTT 
GGCATGCCAG CGTGGTATGT AGCTGCGTGT AGAGCAAATG TGAAATCCGG TGCAATAATG 
TCAGCTTTGA GTGATCAAGA AATCCAGAAG GAGATTGGAA TCTCAAACCC ATTGCACAGG 
TTGAAATTGC GACTTGCAAT TCAGGAAATG GTTTCTTTAA CGTCACCATC TGCCCCACGA 
ACTGCTCGTC TGACTCTTGC CTTCGGAGAT ATGAATCATG AATATATTGG AAACGACTGG 
CTTCCTTGTC TCGGTCTTGC GCAATACCGA TCAGCGTTTA TGGAATGTCT TCTTGACGCT 
CGAATGTTAG AACATCTTTC AAAACGTGAC CTACGCACGC ATCTGCGCAT GGTAGACACA 
TTCCATCGTA CAAGTCTCCA ATACGGAATT ATGTGTCTCA AGAAAGTCAA CTATGATAAG 
AAAGTGTTGG CGGATCGGCG AAAGGCGTGT GATAATATCA ACACAGACTT GCTAGTGTGG 
TCGAATGAAC GAGTTCAGCG ATGGGTGGAA GAAATCGGGC TAGGTGTATT TTCAAGAAAT 
TTAGTGGATA GTGGAATACA TGGTGCATTA ATTGCACTGG ATGAAACGTT TGACGCATCC 
GCTTTTGCAT ATGCACTCCA GATTGGATCT CAAGACGTTC CTAACAGACA ACTACTGGAA 
AAGAAATTCA TTGGCTTAGT CAACGATCAC CGTCAACAAT CCGATCCCCA TCCGAGAAGT 
GGAAGTTCTC GTAAGAATGA TTCCATAGCA AAATCCTACG AGTTTCATTT ATATACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509768.1
CDS	80..3499
Translation	MSYSNGNINCDIMPTISEDGVDNGGPIDEPSDRDNIEQLMMNMLEDRDKLQEQLENYKVQLENAGLRTKEVEKERDMMKRQFEVHTQNLPQELQTMTRELCLLKEQLLEKDEEIVELKAERNNTRLLLEHLECLVSRHERSLRMTVMKRQAQNHAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVAMERVATLEEELSTKGDENSSLKARIATYAAEAEEAMASNAPINGSISSESANRLIEMQEALERMKTELANSLKQSTEITTRNAELEDQLTEDAREKHAAQESIVRLKNQICELDAQRTDQETRITTFESRFLTAQRESTCIRDLNDKLEHQLANKDAAVRLNEEKVHSLQERLELAEKQLAQSLKKAESLPSVEAELQQRMEALTAAEQKSVSAEERIQRLDRNIQELSAELERAVQRERMNEEHSQRLSSTVDKLLSESNDRLQLHLKERMQALDDKNRLTQQLDGTKKIYDQAERIKDRLQRDNESLRQEIEALRQQLYNARTAQFQSRMHAIPFTHAQNIVQQQPQASIAQQSAYQMYKQQPAQQYQTVGMRRPNKGRISALQDDPNKVQTLNEQEWDRLQQAHVLANVQQAFSSSPSLADVGQSTLPRPNTAVQHQQDDMMNSGMGMPSGMQGGMQGGMGGGQDAQMLASMLQDRLDAINTEIRLIQQEKHHAERVAEQLERSSREFYDDQGISTRSSPRASPQLDNMRQHKYNTLPANVSGDRRYDIYGNPQFVDDRMVRDLDYEPRRGYNQFDEMQYERDRMSPASSVASSTDGVLGGKKKRSNSSSGLKTLGRFFNKKKNSSSDLFKRNGDYSDGEQSGTEGNQKADYDRRKKKKHELLEEAMKARTPFALWNGPTVVAWLELWVGMPAWYVAACRANVKSGAIMSALSDQEIQKEIGISNPLHRLKLRLAIQEMVSLTSPSAPRTARLTLAFGDMNHEYIGNDWLPCLGLAQYRSAFMECLLDARMLEHLSKRDLRTHLRMVDTFHRTSLQYGIMCLKKVNYDKKVLADRRKACDNINTDLLVWSNERVQRWVEEIGLGVFSRNLVDSGIHGALIALDETFDASAFAYALQIGSQDVPNRQLLEKKFIGLVNDHRQQSDPHPRSGSSRKNDSIAKSYEFHLYT

Target ORF information:

RefSeq Version	NM_077367.8
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Liprin-alpha (syd-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077367.8

ORF Insert Sequence:

ATGAGCTACA GCAATGGAAA CATAAATTGT GATATAATGC CGACAATCTC GGAAGATGGA 
GTGGACAACG GCGGTCCCAT TGATGAACCA TCGGACCGAG ACAACATTGA GCAGCTGATG 
ATGAACATGT TGGAAGACCG GGACAAATTA CAAGAACAGT TGGAAAATTA TAAGGTGCAA 
CTGGAAAATG CAGGGCTCCG AACGAAAGAA GTTGAAAAAG AACGAGATAT GATGAAGAGA 
CAGTTCGAAG TTCATACGCA AAATTTACCG CAGGAACTTC AAACAATGAC GCGGGAATTA 
TGCCTATTAA AAGAGCAGTT ATTAGAAAAA GATGAAGAGA TTGTCGAGCT CAAAGCAGAA 
CGGAACAATA CTCGACTTCT TCTAGAGCAC CTAGAATGTT TAGTTTCCCG TCATGAAAGA 
TCACTCCGAA TGACGGTCAT GAAGAGACAA GCTCAAAACC ACGCTGGCGT ATCTTCTGAA 
GTTGAAGTTC TAAAAGCTCT TAAGAGTTTA TTCGAGCATC ACAAGGCATT GGATGAAAAA 
GTTCGTGAAC GTCTTCGAGT AGCAATGGAG CGTGTGGCGA CACTTGAAGA AGAGCTGAGC 
ACAAAAGGAG ATGAGAATTC GTCGCTGAAA GCGCGTATTG CCACTTACGC AGCAGAAGCG 
GAAGAAGCAA TGGCGTCAAA TGCACCCATC AATGGTTCAA TTAGCAGTGA AAGTGCAAAC 
AGACTTATCG AAATGCAAGA GGCTTTAGAA AGAATGAAAA CAGAATTAGC CAACTCACTA 
AAACAATCAA CTGAGATCAC AACAAGAAAT GCCGAACTCG AGGATCAATT GACAGAAGAT 
GCTCGTGAAA AACATGCTGC ACAGGAATCA ATCGTTCGTT TGAAGAATCA AATTTGCGAG 
CTTGATGCAC AACGCACCGA TCAAGAAACT CGGATAACAA CGTTTGAGTC GCGTTTCCTG 
ACCGCGCAAA GAGAGTCAAC GTGTATCCGG GACCTCAATG ACAAATTGGA GCATCAGCTT 
GCCAATAAAG ATGCGGCTGT CAGGTTGAAT GAGGAAAAAG TTCATTCACT TCAAGAAAGA 
TTGGAGTTGG CAGAGAAGCA ACTTGCACAA AGTCTCAAAA AAGCTGAATC CCTTCCGTCT 
GTAGAAGCCG AATTACAACA AAGAATGGAA GCACTTACAG CAGCTGAACA GAAGAGTGTT 
TCTGCTGAAG AGCGCATCCA AAGGTTGGAC CGAAACATTC AAGAGTTGTC TGCAGAGTTG 
GAAAGGGCTG TGCAAAGAGA ACGCATGAAC GAAGAGCACA GCCAACGGTT AAGTAGCACT 
GTGGACAAGC TTTTATCGGA ATCTAATGAT CGCCTTCAAC TGCATCTCAA AGAACGAATG 
CAAGCCCTCG ATGACAAAAA TCGGTTAACA CAACAGCTTG ATGGAACAAA GAAAATTTAT 
GATCAAGCGG AACGAATCAA AGATCGTCTT CAGCGGGATA ATGAATCTCT ACGGCAAGAG 
ATTGAAGCTC TTAGGCAACA ATTGTACAAT GCAAGAACTG CGCAATTCCA GTCAAGAATG 
CATGCAATTC CGTTTACACA TGCTCAAAAC ATTGTGCAGC AACAACCACA AGCTTCGATT 
GCTCAGCAAT CTGCATACCA AATGTACAAA CAACAACCAG CTCAACAGTA TCAAACTGTA 
GGAATGAGAA GACCAAATAA AGGAAGGATT TCAGCACTCC AGGACGATCC GAACAAAGTT 
CAAACCCTCA ACGAGCAAGA ATGGGATCGA CTTCAACAAG CGCATGTTCT TGCCAACGTC 
CAGCAAGCAT TTTCCTCATC ACCATCACTG GCCGACGTCG GTCAATCGAC TCTTCCACGT 
CCTAACACTG CTGTCCAACA CCAGCAAGAT GATATGATGA ACAGTGGAAT GGGAATGCCA 
AGTGGAATGC AGGGAGGAAT GCAAGGTGGA ATGGGTGGTG GTCAAGATGC TCAGATGTTG 
GCATCAATGC TACAAGATCG CTTAGATGCA ATAAATACTG AAATAAGATT GATTCAACAA 
GAGAAACATC ACGCAGAACG GGTGGCTGAA CAGTTAGAAC GATCAAGTCG GGAATTCTAT 
GATGATCAGG GAATATCTAC GAGAAGTTCA CCAAGAGCAA GTCCCCAATT AGATAATATG 
CGACAACATA AGTATAACAC TCTTCCTGCA AATGTATCCG GTGACCGACG GTATGATATT 
TACGGCAATC CGCAGTTTGT TGACGACCGC ATGGTTAGAG ATTTGGACTA CGAGCCACGA 
CGAGGGTACA ACCAATTTGA CGAGATGCAA TACGAAAGAG ATCGAATGAG TCCAGCTTCG 
TCAGTCGCTT CCTCGACAGA TGGCGTTCTT GGCGGAAAGA AGAAAAGATC GAATAGCTCA 
AGTGGATTGA AAACGCTTGG AAGGTTTTTC AACAAAAAGA AAAATTCATC GAGTGATTTA 
TTCAAACGAA ATGGAGATTA TTCGGATGGC GAGCAGTCGG GCACAGAAGG AAACCAAAAA 
GCTGATTACG ACAGGAGGAA AAAGAAGAAG CATGAACTAT TAGAGGAAGC CATGAAAGCA 
CGAACACCGT TTGCTCTTTG GAATGGTCCA ACTGTTGTTG CCTGGCTGGA GCTGTGGGTT 
GGCATGCCAG CGTGGTATGT AGCTGCGTGT AGAGCAAATG TGAAATCCGG TGCAATAATG 
TCAGCTTTGA GTGATCAAGA AATCCAGAAG GAGATTGGAA TCTCAAACCC ATTGCACAGG 
TTGAAATTGC GACTTGCAAT TCAGGAAATG GTTTCTTTAA CGTCACCATC TGCCCCACGA 
ACTGCTCGTC TGACTCTTGC CTTCGGAGAT ATGAATCATG AATATATTGG AAACGACTGG 
CTTCCTTGTC TCGGTCTTGC GCAATACCGA TCAGCGTTTA TGGAATGTCT TCTTGACGCT 
CGAATGTTAG AACATCTTTC AAAACGTGAC CTACGCACGC ATCTGCGCAT GGTAGACACA 
TTCCATCGTA CAAGTCTCCA ATACGGAATT ATGTGTCTCA AGAAAGTCAA CTATGATAAG 
AAAGTGTTGG CGGATCGGCG AAAGGCGTGT GATAATATCA ACACAGACTT GCTAGTGTGG 
TCGAATGAAC GAGTTCAGCG ATGGGTGGAA GAAATCGGGC TAGGTGTATT TTCAAGAAAT 
TTAGTGGATA GTGGAATACA TGGTGCATTA ATTGCACTGG ATGAAACGTT TGACGCATCC 
GCTTTTGCAT ATGCACTCCA GATTGGATCT CAAGACGTTC CTAACAGACA ACTACTGGAA 
AAGAAATTCA TTGGCTTAGT CAACGATCAC CGTCAACAAT CCGATCCCCA TCCGAGAAGT 
GGAAGTTCTC GTAAGAATGA TTCCATAGCA AAATCCTACG AGTTTCATTT ATATACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

syd-2 ( NM_077367.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509768.1 Caenorhabditis elegans Liprin-alpha (syd-2), partial mRNA.

Price & Availability↑

CloneID	OCa350140
Clone ID Related Accession (Same CDS sequence)	NM_077367.8 , NM_077367.6
Accession Version	NM_077367.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Liprin-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_077367.5. COMPLETENESS: incomplete on both ends.

ATGAGCTACA GCAATGGAAA CATAAATTGT GATATAATGC CGACAATCTC GGAAGATGGA 
GTGGACAACG GCGGTCCCAT TGATGAACCA TCGGACCGAG ACAACATTGA GCAGCTGATG 
ATGAACATGT TGGAAGACCG GGACAAATTA CAAGAACAGT TGGAAAATTA TAAGGTGCAA 
CTGGAAAATG CAGGGCTCCG AACGAAAGAA GTTGAAAAAG AACGAGATAT GATGAAGAGA 
CAGTTCGAAG TTCATACGCA AAATTTACCG CAGGAACTTC AAACAATGAC GCGGGAATTA 
TGCCTATTAA AAGAGCAGTT ATTAGAAAAA GATGAAGAGA TTGTCGAGCT CAAAGCAGAA 
CGGAACAATA CTCGACTTCT TCTAGAGCAC CTAGAATGTT TAGTTTCCCG TCATGAAAGA 
TCACTCCGAA TGACGGTCAT GAAGAGACAA GCTCAAAACC ACGCTGGCGT ATCTTCTGAA 
GTTGAAGTTC TAAAAGCTCT TAAGAGTTTA TTCGAGCATC ACAAGGCATT GGATGAAAAA 
GTTCGTGAAC GTCTTCGAGT AGCAATGGAG CGTGTGGCGA CACTTGAAGA AGAGCTGAGC 
ACAAAAGGAG ATGAGAATTC GTCGCTGAAA GCGCGTATTG CCACTTACGC AGCAGAAGCG 
GAAGAAGCAA TGGCGTCAAA TGCACCCATC AATGGTTCAA TTAGCAGTGA AAGTGCAAAC 
AGACTTATCG AAATGCAAGA GGCTTTAGAA AGAATGAAAA CAGAATTAGC CAACTCACTA 
AAACAATCAA CTGAGATCAC AACAAGAAAT GCCGAACTCG AGGATCAATT GACAGAAGAT 
GCTCGTGAAA AACATGCTGC ACAGGAATCA ATCGTTCGTT TGAAGAATCA AATTTGCGAG 
CTTGATGCAC AACGCACCGA TCAAGAAACT CGGATAACAA CGTTTGAGTC GCGTTTCCTG 
ACCGCGCAAA GAGAGTCAAC GTGTATCCGG GACCTCAATG ACAAATTGGA GCATCAGCTT 
GCCAATAAAG ATGCGGCTGT CAGGTTGAAT GAGGAAAAAG TTCATTCACT TCAAGAAAGA 
TTGGAGTTGG CAGAGAAGCA ACTTGCACAA AGTCTCAAAA AAGCTGAATC CCTTCCGTCT 
GTAGAAGCCG AATTACAACA AAGAATGGAA GCACTTACAG CAGCTGAACA GAAGAGTGTT 
TCTGCTGAAG AGCGCATCCA AAGGTTGGAC CGAAACATTC AAGAGTTGTC TGCAGAGTTG 
GAAAGGGCTG TGCAAAGAGA ACGCATGAAC GAAGAGCACA GCCAACGGTT AAGTAGCACT 
GTGGACAAGC TTTTATCGGA ATCTAATGAT CGCCTTCAAC TGCATCTCAA AGAACGAATG 
CAAGCCCTCG ATGACAAAAA TCGGTTAACA CAACAGCTTG ATGGAACAAA GAAAATTTAT 
GATCAAGCGG AACGAATCAA AGATCGTCTT CAGCGGGATA ATGAATCTCT ACGGCAAGAG 
ATTGAAGCTC TTAGGCAACA ATTGTACAAT GCAAGAACTG CGCAATTCCA GTCAAGAATG 
CATGCAATTC CGTTTACACA TGCTCAAAAC ATTGTGCAGC AACAACCACA AGCTTCGATT 
GCTCAGCAAT CTGCATACCA AATGTACAAA CAACAACCAG CTCAACAGTA TCAAACTGTA 
GGAATGAGAA GACCAAATAA AGGAAGGATT TCAGCACTCC AGGACGATCC GAACAAAGTT 
CAAACCCTCA ACGAGCAAGA ATGGGATCGA CTTCAACAAG CGCATGTTCT TGCCAACGTC 
CAGCAAGCAT TTTCCTCATC ACCATCACTG GCCGACGTCG GTCAATCGAC TCTTCCACGT 
CCTAACACTG CTGTCCAACA CCAGCAAGAT GATATGATGA ACAGTGGAAT GGGAATGCCA 
AGTGGAATGC AGGGAGGAAT GCAAGGTGGA ATGGGTGGTG GTCAAGATGC TCAGATGTTG 
GCATCAATGC TACAAGATCG CTTAGATGCA ATAAATACTG AAATAAGATT GATTCAACAA 
GAGAAACATC ACGCAGAACG GGTGGCTGAA CAGTTAGAAC GATCAAGTCG GGAATTCTAT 
GATGATCAGG GAATATCTAC GAGAAGTTCA CCAAGAGCAA GTCCCCAATT AGATAATATG 
CGACAACATA AGTATAACAC TCTTCCTGCA AATGTATCCG GTGACCGACG GTATGATATT 
TACGGCAATC CGCAGTTTGT TGACGACCGC ATGGTTAGAG ATTTGGACTA CGAGCCACGA 
CGAGGGTACA ACCAATTTGA CGAGATGCAA TACGAAAGAG ATCGAATGAG TCCAGCTTCG 
TCAGTCGCTT CCTCGACAGA TGGCGTTCTT GGCGGAAAGA AGAAAAGATC GAATAGCTCA 
AGTGGATTGA AAACGCTTGG AAGGTTTTTC AACAAAAAGA AAAATTCATC GAGTGATTTA 
TTCAAACGAA ATGGAGATTA TTCGGATGGC GAGCAGTCGG GCACAGAAGG AAACCAAAAA 
GCTGATTACG ACAGGAGGAA AAAGAAGAAG CATGAACTAT TAGAGGAAGC CATGAAAGCA 
CGAACACCGT TTGCTCTTTG GAATGGTCCA ACTGTTGTTG CCTGGCTGGA GCTGTGGGTT 
GGCATGCCAG CGTGGTATGT AGCTGCGTGT AGAGCAAATG TGAAATCCGG TGCAATAATG 
TCAGCTTTGA GTGATCAAGA AATCCAGAAG GAGATTGGAA TCTCAAACCC ATTGCACAGG 
TTGAAATTGC GACTTGCAAT TCAGGAAATG GTTTCTTTAA CGTCACCATC TGCCCCACGA 
ACTGCTCGTC TGACTCTTGC CTTCGGAGAT ATGAATCATG AATATATTGG AAACGACTGG 
CTTCCTTGTC TCGGTCTTGC GCAATACCGA TCAGCGTTTA TGGAATGTCT TCTTGACGCT 
CGAATGTTAG AACATCTTTC AAAACGTGAC CTACGCACGC ATCTGCGCAT GGTAGACACA 
TTCCATCGTA CAAGTCTCCA ATACGGAATT ATGTGTCTCA AGAAAGTCAA CTATGATAAG 
AAAGTGTTGG CGGATCGGCG AAAGGCGTGT GATAATATCA ACACAGACTT GCTAGTGTGG 
TCGAATGAAC GAGTTCAGCG ATGGGTGGAA GAAATCGGGC TAGGTGTATT TTCAAGAAAT 
TTAGTGGATA GTGGAATACA TGGTGCATTA ATTGCACTGG ATGAAACGTT TGACGCATCC 
GCTTTTGCAT ATGCACTCCA GATTGGATCT CAAGACGTTC CTAACAGACA ACTACTGGAA 
AAGAAATTCA TTGGCTTAGT CAACGATCAC CGTCAACAAT CCGATCCCCA TCCGAGAAGT 
GGAAGTTCTC GTAAGAATGA TTCCATAGCA AAATCCTACG AGTTTCATTT ATATACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509768.1
CDS	1..3420
Translation	MSYSNGNINCDIMPTISEDGVDNGGPIDEPSDRDNIEQLMMNMLEDRDKLQEQLENYKVQLENAGLRTKEVEKERDMMKRQFEVHTQNLPQELQTMTRELCLLKEQLLEKDEEIVELKAERNNTRLLLEHLECLVSRHERSLRMTVMKRQAQNHAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVAMERVATLEEELSTKGDENSSLKARIATYAAEAEEAMASNAPINGSISSESANRLIEMQEALERMKTELANSLKQSTEITTRNAELEDQLTEDAREKHAAQESIVRLKNQICELDAQRTDQETRITTFESRFLTAQRESTCIRDLNDKLEHQLANKDAAVRLNEEKVHSLQERLELAEKQLAQSLKKAESLPSVEAELQQRMEALTAAEQKSVSAEERIQRLDRNIQELSAELERAVQRERMNEEHSQRLSSTVDKLLSESNDRLQLHLKERMQALDDKNRLTQQLDGTKKIYDQAERIKDRLQRDNESLRQEIEALRQQLYNARTAQFQSRMHAIPFTHAQNIVQQQPQASIAQQSAYQMYKQQPAQQYQTVGMRRPNKGRISALQDDPNKVQTLNEQEWDRLQQAHVLANVQQAFSSSPSLADVGQSTLPRPNTAVQHQQDDMMNSGMGMPSGMQGGMQGGMGGGQDAQMLASMLQDRLDAINTEIRLIQQEKHHAERVAEQLERSSREFYDDQGISTRSSPRASPQLDNMRQHKYNTLPANVSGDRRYDIYGNPQFVDDRMVRDLDYEPRRGYNQFDEMQYERDRMSPASSVASSTDGVLGGKKKRSNSSSGLKTLGRFFNKKKNSSSDLFKRNGDYSDGEQSGTEGNQKADYDRRKKKKHELLEEAMKARTPFALWNGPTVVAWLELWVGMPAWYVAACRANVKSGAIMSALSDQEIQKEIGISNPLHRLKLRLAIQEMVSLTSPSAPRTARLTLAFGDMNHEYIGNDWLPCLGLAQYRSAFMECLLDARMLEHLSKRDLRTHLRMVDTFHRTSLQYGIMCLKKVNYDKKVLADRRKACDNINTDLLVWSNERVQRWVEEIGLGVFSRNLVDSGIHGALIALDETFDASAFAYALQIGSQDVPNRQLLEKKFIGLVNDHRQQSDPHPRSGSSRKNDSIAKSYEFHLYT

Target ORF information:

RefSeq Version	NM_077367.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Liprin-alpha (syd-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077367.6

ORF Insert Sequence:

ATGAGCTACA GCAATGGAAA CATAAATTGT GATATAATGC CGACAATCTC GGAAGATGGA 
GTGGACAACG GCGGTCCCAT TGATGAACCA TCGGACCGAG ACAACATTGA GCAGCTGATG 
ATGAACATGT TGGAAGACCG GGACAAATTA CAAGAACAGT TGGAAAATTA TAAGGTGCAA 
CTGGAAAATG CAGGGCTCCG AACGAAAGAA GTTGAAAAAG AACGAGATAT GATGAAGAGA 
CAGTTCGAAG TTCATACGCA AAATTTACCG CAGGAACTTC AAACAATGAC GCGGGAATTA 
TGCCTATTAA AAGAGCAGTT ATTAGAAAAA GATGAAGAGA TTGTCGAGCT CAAAGCAGAA 
CGGAACAATA CTCGACTTCT TCTAGAGCAC CTAGAATGTT TAGTTTCCCG TCATGAAAGA 
TCACTCCGAA TGACGGTCAT GAAGAGACAA GCTCAAAACC ACGCTGGCGT ATCTTCTGAA 
GTTGAAGTTC TAAAAGCTCT TAAGAGTTTA TTCGAGCATC ACAAGGCATT GGATGAAAAA 
GTTCGTGAAC GTCTTCGAGT AGCAATGGAG CGTGTGGCGA CACTTGAAGA AGAGCTGAGC 
ACAAAAGGAG ATGAGAATTC GTCGCTGAAA GCGCGTATTG CCACTTACGC AGCAGAAGCG 
GAAGAAGCAA TGGCGTCAAA TGCACCCATC AATGGTTCAA TTAGCAGTGA AAGTGCAAAC 
AGACTTATCG AAATGCAAGA GGCTTTAGAA AGAATGAAAA CAGAATTAGC CAACTCACTA 
AAACAATCAA CTGAGATCAC AACAAGAAAT GCCGAACTCG AGGATCAATT GACAGAAGAT 
GCTCGTGAAA AACATGCTGC ACAGGAATCA ATCGTTCGTT TGAAGAATCA AATTTGCGAG 
CTTGATGCAC AACGCACCGA TCAAGAAACT CGGATAACAA CGTTTGAGTC GCGTTTCCTG 
ACCGCGCAAA GAGAGTCAAC GTGTATCCGG GACCTCAATG ACAAATTGGA GCATCAGCTT 
GCCAATAAAG ATGCGGCTGT CAGGTTGAAT GAGGAAAAAG TTCATTCACT TCAAGAAAGA 
TTGGAGTTGG CAGAGAAGCA ACTTGCACAA AGTCTCAAAA AAGCTGAATC CCTTCCGTCT 
GTAGAAGCCG AATTACAACA AAGAATGGAA GCACTTACAG CAGCTGAACA GAAGAGTGTT 
TCTGCTGAAG AGCGCATCCA AAGGTTGGAC CGAAACATTC AAGAGTTGTC TGCAGAGTTG 
GAAAGGGCTG TGCAAAGAGA ACGCATGAAC GAAGAGCACA GCCAACGGTT AAGTAGCACT 
GTGGACAAGC TTTTATCGGA ATCTAATGAT CGCCTTCAAC TGCATCTCAA AGAACGAATG 
CAAGCCCTCG ATGACAAAAA TCGGTTAACA CAACAGCTTG ATGGAACAAA GAAAATTTAT 
GATCAAGCGG AACGAATCAA AGATCGTCTT CAGCGGGATA ATGAATCTCT ACGGCAAGAG 
ATTGAAGCTC TTAGGCAACA ATTGTACAAT GCAAGAACTG CGCAATTCCA GTCAAGAATG 
CATGCAATTC CGTTTACACA TGCTCAAAAC ATTGTGCAGC AACAACCACA AGCTTCGATT 
GCTCAGCAAT CTGCATACCA AATGTACAAA CAACAACCAG CTCAACAGTA TCAAACTGTA 
GGAATGAGAA GACCAAATAA AGGAAGGATT TCAGCACTCC AGGACGATCC GAACAAAGTT 
CAAACCCTCA ACGAGCAAGA ATGGGATCGA CTTCAACAAG CGCATGTTCT TGCCAACGTC 
CAGCAAGCAT TTTCCTCATC ACCATCACTG GCCGACGTCG GTCAATCGAC TCTTCCACGT 
CCTAACACTG CTGTCCAACA CCAGCAAGAT GATATGATGA ACAGTGGAAT GGGAATGCCA 
AGTGGAATGC AGGGAGGAAT GCAAGGTGGA ATGGGTGGTG GTCAAGATGC TCAGATGTTG 
GCATCAATGC TACAAGATCG CTTAGATGCA ATAAATACTG AAATAAGATT GATTCAACAA 
GAGAAACATC ACGCAGAACG GGTGGCTGAA CAGTTAGAAC GATCAAGTCG GGAATTCTAT 
GATGATCAGG GAATATCTAC GAGAAGTTCA CCAAGAGCAA GTCCCCAATT AGATAATATG 
CGACAACATA AGTATAACAC TCTTCCTGCA AATGTATCCG GTGACCGACG GTATGATATT 
TACGGCAATC CGCAGTTTGT TGACGACCGC ATGGTTAGAG ATTTGGACTA CGAGCCACGA 
CGAGGGTACA ACCAATTTGA CGAGATGCAA TACGAAAGAG ATCGAATGAG TCCAGCTTCG 
TCAGTCGCTT CCTCGACAGA TGGCGTTCTT GGCGGAAAGA AGAAAAGATC GAATAGCTCA 
AGTGGATTGA AAACGCTTGG AAGGTTTTTC AACAAAAAGA AAAATTCATC GAGTGATTTA 
TTCAAACGAA ATGGAGATTA TTCGGATGGC GAGCAGTCGG GCACAGAAGG AAACCAAAAA 
GCTGATTACG ACAGGAGGAA AAAGAAGAAG CATGAACTAT TAGAGGAAGC CATGAAAGCA 
CGAACACCGT TTGCTCTTTG GAATGGTCCA ACTGTTGTTG CCTGGCTGGA GCTGTGGGTT 
GGCATGCCAG CGTGGTATGT AGCTGCGTGT AGAGCAAATG TGAAATCCGG TGCAATAATG 
TCAGCTTTGA GTGATCAAGA AATCCAGAAG GAGATTGGAA TCTCAAACCC ATTGCACAGG 
TTGAAATTGC GACTTGCAAT TCAGGAAATG GTTTCTTTAA CGTCACCATC TGCCCCACGA 
ACTGCTCGTC TGACTCTTGC CTTCGGAGAT ATGAATCATG AATATATTGG AAACGACTGG 
CTTCCTTGTC TCGGTCTTGC GCAATACCGA TCAGCGTTTA TGGAATGTCT TCTTGACGCT 
CGAATGTTAG AACATCTTTC AAAACGTGAC CTACGCACGC ATCTGCGCAT GGTAGACACA 
TTCCATCGTA CAAGTCTCCA ATACGGAATT ATGTGTCTCA AGAAAGTCAA CTATGATAAG 
AAAGTGTTGG CGGATCGGCG AAAGGCGTGT GATAATATCA ACACAGACTT GCTAGTGTGG 
TCGAATGAAC GAGTTCAGCG ATGGGTGGAA GAAATCGGGC TAGGTGTATT TTCAAGAAAT 
TTAGTGGATA GTGGAATACA TGGTGCATTA ATTGCACTGG ATGAAACGTT TGACGCATCC 
GCTTTTGCAT ATGCACTCCA GATTGGATCT CAAGACGTTC CTAACAGACA ACTACTGGAA 
AAGAAATTCA TTGGCTTAGT CAACGATCAC CGTCAACAAT CCGATCCCCA TCCGAGAAGT 
GGAAGTTCTC GTAAGAATGA TTCCATAGCA AAATCCTACG AGTTTCATTT ATATACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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syd-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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