ppfia2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppfia2
Entrez Gene ID	100489336
Full Name	protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
General protein information	Preferred Names protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 Names liprin-alpha-2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031898887.1	XP_031754747.1	liprin-alpha-2 isoform X1
XM_031898888.1	XP_031754748.1	liprin-alpha-2 isoform X2
XM_012960179.2	XP_012815633.1	liprin-alpha-2
XM_031898889.1	XP_031754749.1	liprin-alpha-2 isoform X3
XM_031898890.1	XP_031754750.1	liprin-alpha-2 isoform X4
XM_031898891.1	XP_031754751.1	liprin-alpha-2 isoform X5

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPFIA2	NP_003616.2
Canis lupus familiaris (dog)	PPFIA2	XP_003432068.2
Xenopus tropicalis (tropical clawed frog)	ppfia2	XP_002931667.2
Rattus norvegicus (Norway rat)	Ppfia2	NP_001102215.1
Danio rerio (zebrafish)	ppfia2	NP_001038576.1
Caenorhabditis elegans (roundworm)	syd-2	NP_509768.1
Mus musculus (house mouse)	Ppfia2	NP_001192270.1
	PPFIA2	XP_001088353.2
Gallus gallus (chicken)	PPFIA2	XP_416120.4
Pan troglodytes (chimpanzee)	PPFIA2	XP_509243.2
Drosophila melanogaster (fruit fly)	Liprin-alpha	NP_001260155.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001469	XP_321656.5

Gene Ontology

The following ppfia2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppfia2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa54658	XM_031898887.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54659	XM_031898888.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54660	XM_031898889.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54661	XM_031898890.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa54662	XM_031898891.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa34482	XM_012960179.2 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppfia2 ( XM_031898887.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754747.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54658
Clone ID Related Accession (Same CDS sequence)	XM_031898887.1
Accession Version	XM_031898887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	liprin-alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAGCTCGAC AACGGGAAAA AATGAATGAA 
GAGCACAACA AAAGATTGTC TGATACAGTG GACCGGCTTT TAACAGAGTC CAATGAACGA 
CTCCAGTTAC ACTTGAAAGA ACGAATGGCT GCACTAGAAG ACAAGAATAT TTTACTTCAG 
GAATCAGAGA CCTTCAGAAA ACAGCTAGAA GAATGCTTGC ATGATAAGGA TCGGCTACAA 
GAAGAAGTTG AAAAATTAAG ATCTGAGGTT GATCAAGTGA AGATAAGAAC AGGGTCCTTG 
ATAGAACCTA CATTAACGAG GCCTCACCTT GACTCATCTG CAGAGTTAAG ATATTCAGTA 
GGGTCTCTTG TTGACAGCCA GTCTGACTAC CGCTCAACTA AAGTCATACG AAGACCAAGA 
AGAGGTAGAA TGGGCGTTAG GAGAGAAGAG CAACCGGTGA AATCACTTGG GGACCATGAA 
TGGAACCGAA CACAGCAGAT TGGAGTCCTT AGCAGCCATG CATTTGAGAG TGATACTGAA 
ATGTCCGACA TAGATGATGA TGACCGTGAA ACAATTTTTA GTTCCATGGA CCTCTTATCT 
CCAGGTGGTC ACTCTGATGC TCAAACTTTA GCCATGATGC TTCAAGAACA GCTAGATGCC 
ATCAATAAAG AAATAAGACT CATTCAGGAA GAAAAGGAAT CTACTGAACT TCGTGCAGAA 
GAAATTGAAA ATAGAGTAGC CAGTGTTAGC CTAGAGGGGC TCAACCTGGC CAGAATGCAC 
CCAGGTACAT CGATCACTGC TTCAGTCACT GCTTCATCAT TAGCAAGTTC ATCACCTCCA 
AGTGGTCATT CTACACCCAA ACTCACACCT CGTAGTCCAG CAAGAGAAAT GGATCGAATG 
GGGGTCATGA CACTACCAAG TGATTTGAGA AAGCATCGAA GAAAGATTGC AGTTGTAGAA 
GAAGATGGGC GTGAAGATAA AGCCACAATA AAATGCGAAA CATCCCCTCC TTCAACACCA 
CGTGCTCTCA GGATGACACA TACCTTGCCA TCTTCATACC ATAATGATGC CAGAGGAAGT 
CTGACTGCTT CTCTTGAACC TGATGTGTTT GGTCTTGGGA GCACAAACAG TAGCCAAGAC 
TCTCTGCACA AAGCTCCAAA GAAGAAAGGA ATCAAGTCAT CCATTGGCCG GTTGTTTGGT 
AAAAAAGAGA AGGCTCGATT CGGACAGCTC AGTAAGGAAT TTGGTCTACA AGGTCAAGGC 
GCATTTATGG ATGCTGATCA AGCGGCACAA GATTCCTTGG GCTTAGGAAA ACTTGGAACT 
CAAGCAGAAA AAGACAGAAG GCTCAAAAAG AAACATGAAC TTCTGGAAGA AGCAAGAAGA 
AAGGGACTTC CATTTGCACA GTGGGATGGG CCAACCGTAG TTGCATGGCT TGAACTTTGG 
TTGGGAATGC CAGCTTGGTA TGTTGCTGCA TGTCGTGCCA ACGTAAAGAG TGGTGCCATA 
ATGTCTGCCT TGTCTGATAC GGAAATACAA AGGGAGATTG GGATTAGCAA TCCTCTGCAT 
CGTTTAAAGC TACGGTTAGC AATCCAAGAG ATGGTTTCAC TCACCAGCCC ATCAGCGCCT 
CCAACATCCA GAACACCATC AGGTAATGTC TGGGTAACCC ATGAGGAAAT GGAGAACCTT 
ACTGCTCCCA CCAAAACGAA AGAGTCAGAA GAAGGCAGCT GGGCACAAAC ATTGGCATAT 
GGCGATATGA ACCATGAGTG GATTGGGAAT GAATGGCTTC CTAGTCTGGG CTTACCGCAG 
TACAGAAGTT ACTTCATGGA ATGCCTTGTA GATGCCCGGA TGCTAGACCA TTTAACAAAA 
AAAGATCTTC GAGTGCACTT AAAAATGGTG GATAGCTTTC ATCGAACAAG TTTGCAGTAT 
GGCATAATGT GCTTGAAAAA ATTAAACTAT GATAGGAAGG AGCTGGAAAG GAGAAGAGAA 
ACAAGCCAGC ATGAAATTAA AGATGTGTTA GTGTGGAGCA ATGATCGTGT GATTCGCTGG 
ATCCAAGCAA TAGGCCTTAG AGATTATGCA AACAACATAC TGGAGAGTGG AATCCACGGG 
TCACTTATTG CCTTGGATGA GAACTTTGAC TACACAAGTT TAGCTCTTTT GTTACAAATC 
CCAACACAAA ATACTCAGGC ACGGCAGATC CTGGAAAGAG AATACAATAA TTTATTGGCT 
TTGGGAACTG AAAGGCGCCT TGATGAGAGT GATGACAAGA ACTTCAGACG TGGGCCCTCC 
TGGAGGAGGC AGTTTCCACC ACGTGAAGTG CACGGAATCA GCATGATGCC AGGTTCTTCG 
GAAACACTGC CAGCAGGATT CAGATTAACA ACAACATCCG GGCATTCACG GAAAATGACA 
ACAGATGTTG CCCCTTCAGG AATACAGAGA TTAGACAGCT CCACGGTCCG TACATACTCA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754747.1
CDS	322..4047
Translation	MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLGLAQQRLQDVIYDRDSLQRQLNSALPQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELTAANQEIVALREQNAHIQRKMGTGEGPEQEALEGMEQGQKIHDKRLSNGSIDSNDDASQMVELQELLEKQNYEMTQMKERLSSLSARVGEVEQEAESARKELVKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQREATSIHDINDKLENELANKEAILRQMEEKNRQLQERLEMAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEDKNILLQESETFRKQLEECLHDKDRLQEEVEKLRSEVDQVKIRTGSLIEPTLTRPHLDSSAELRYSVGSLVDSQSDYRSTKVIRRPRRGRMGVRREEQPVKSLGDHEWNRTQQIGVLSSHAFESDTEMSDIDDDDRETIFSSMDLLSPGGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPSTPRALRMTHTLPSSYHNDARGSLTASLEPDVFGLGSTNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARFGQLSKEFGLQGQGAFMDADQAAQDSLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLTAPTKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRKELERRRETSQHEIKDVLVWSNDRVIRWIQAIGLRDYANNILESGIHGSLIALDENFDYTSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGPSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGHSRKMTTDVAPSGIQRLDSSTVRTYSC

Target ORF information:

RefSeq Version	XM_031898887.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898887.1

ORF Insert Sequence:

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAGCTCGAC AACGGGAAAA AATGAATGAA 
GAGCACAACA AAAGATTGTC TGATACAGTG GACCGGCTTT TAACAGAGTC CAATGAACGA 
CTCCAGTTAC ACTTGAAAGA ACGAATGGCT GCACTAGAAG ACAAGAATAT TTTACTTCAG 
GAATCAGAGA CCTTCAGAAA ACAGCTAGAA GAATGCTTGC ATGATAAGGA TCGGCTACAA 
GAAGAAGTTG AAAAATTAAG ATCTGAGGTT GATCAAGTGA AGATAAGAAC AGGGTCCTTG 
ATAGAACCTA CATTAACGAG GCCTCACCTT GACTCATCTG CAGAGTTAAG ATATTCAGTA 
GGGTCTCTTG TTGACAGCCA GTCTGACTAC CGCTCAACTA AAGTCATACG AAGACCAAGA 
AGAGGTAGAA TGGGCGTTAG GAGAGAAGAG CAACCGGTGA AATCACTTGG GGACCATGAA 
TGGAACCGAA CACAGCAGAT TGGAGTCCTT AGCAGCCATG CATTTGAGAG TGATACTGAA 
ATGTCCGACA TAGATGATGA TGACCGTGAA ACAATTTTTA GTTCCATGGA CCTCTTATCT 
CCAGGTGGTC ACTCTGATGC TCAAACTTTA GCCATGATGC TTCAAGAACA GCTAGATGCC 
ATCAATAAAG AAATAAGACT CATTCAGGAA GAAAAGGAAT CTACTGAACT TCGTGCAGAA 
GAAATTGAAA ATAGAGTAGC CAGTGTTAGC CTAGAGGGGC TCAACCTGGC CAGAATGCAC 
CCAGGTACAT CGATCACTGC TTCAGTCACT GCTTCATCAT TAGCAAGTTC ATCACCTCCA 
AGTGGTCATT CTACACCCAA ACTCACACCT CGTAGTCCAG CAAGAGAAAT GGATCGAATG 
GGGGTCATGA CACTACCAAG TGATTTGAGA AAGCATCGAA GAAAGATTGC AGTTGTAGAA 
GAAGATGGGC GTGAAGATAA AGCCACAATA AAATGCGAAA CATCCCCTCC TTCAACACCA 
CGTGCTCTCA GGATGACACA TACCTTGCCA TCTTCATACC ATAATGATGC CAGAGGAAGT 
CTGACTGCTT CTCTTGAACC TGATGTGTTT GGTCTTGGGA GCACAAACAG TAGCCAAGAC 
TCTCTGCACA AAGCTCCAAA GAAGAAAGGA ATCAAGTCAT CCATTGGCCG GTTGTTTGGT 
AAAAAAGAGA AGGCTCGATT CGGACAGCTC AGTAAGGAAT TTGGTCTACA AGGTCAAGGC 
GCATTTATGG ATGCTGATCA AGCGGCACAA GATTCCTTGG GCTTAGGAAA ACTTGGAACT 
CAAGCAGAAA AAGACAGAAG GCTCAAAAAG AAACATGAAC TTCTGGAAGA AGCAAGAAGA 
AAGGGACTTC CATTTGCACA GTGGGATGGG CCAACCGTAG TTGCATGGCT TGAACTTTGG 
TTGGGAATGC CAGCTTGGTA TGTTGCTGCA TGTCGTGCCA ACGTAAAGAG TGGTGCCATA 
ATGTCTGCCT TGTCTGATAC GGAAATACAA AGGGAGATTG GGATTAGCAA TCCTCTGCAT 
CGTTTAAAGC TACGGTTAGC AATCCAAGAG ATGGTTTCAC TCACCAGCCC ATCAGCGCCT 
CCAACATCCA GAACACCATC AGGTAATGTC TGGGTAACCC ATGAGGAAAT GGAGAACCTT 
ACTGCTCCCA CCAAAACGAA AGAGTCAGAA GAAGGCAGCT GGGCACAAAC ATTGGCATAT 
GGCGATATGA ACCATGAGTG GATTGGGAAT GAATGGCTTC CTAGTCTGGG CTTACCGCAG 
TACAGAAGTT ACTTCATGGA ATGCCTTGTA GATGCCCGGA TGCTAGACCA TTTAACAAAA 
AAAGATCTTC GAGTGCACTT AAAAATGGTG GATAGCTTTC ATCGAACAAG TTTGCAGTAT 
GGCATAATGT GCTTGAAAAA ATTAAACTAT GATAGGAAGG AGCTGGAAAG GAGAAGAGAA 
ACAAGCCAGC ATGAAATTAA AGATGTGTTA GTGTGGAGCA ATGATCGTGT GATTCGCTGG 
ATCCAAGCAA TAGGCCTTAG AGATTATGCA AACAACATAC TGGAGAGTGG AATCCACGGG 
TCACTTATTG CCTTGGATGA GAACTTTGAC TACACAAGTT TAGCTCTTTT GTTACAAATC 
CCAACACAAA ATACTCAGGC ACGGCAGATC CTGGAAAGAG AATACAATAA TTTATTGGCT 
TTGGGAACTG AAAGGCGCCT TGATGAGAGT GATGACAAGA ACTTCAGACG TGGGCCCTCC 
TGGAGGAGGC AGTTTCCACC ACGTGAAGTG CACGGAATCA GCATGATGCC AGGTTCTTCG 
GAAACACTGC CAGCAGGATT CAGATTAACA ACAACATCCG GGCATTCACG GAAAATGACA 
ACAGATGTTG CCCCTTCAGG AATACAGAGA TTAGACAGCT CCACGGTCCG TACATACTCA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia2 ( XM_031898888.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754748.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54659
Clone ID Related Accession (Same CDS sequence)	XM_031898888.1
Accession Version	XM_031898888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	liprin-alpha-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAGCTCGAC AACGGGAAAA AATGAATGAA 
GAGCACAACA AAAGATTGTC TGATACAGTG GACCGGCTTT TAACAGAGTC CAATGAACGA 
CTCCAGTTAC ACTTGAAAGA ACGAATGGCT GCACTAGAAG ACAAGAATAT TTTACTTCAG 
GAATCAGAGA CCTTCAGAAA ACAGCTAGAA GAATGCTTGC ATGATAAGGA TCGGCTACAA 
GAAGAAGTTG AAAAATTAAG ATCTGAGGTT GATCAAGTGA AGATAAGAAC AGGGTCCTTG 
ATAGAACCTA CATTAACGAG GCCTCACCTT GACTCATCTG CAGAGTTAAG ATATTCAGTA 
GGGTCTCTTG TTGACAGCCA GTCTGACTAC CGCTCAACTA AAGTCATACG AAGACCAAGA 
AGAGGTAGAA TGGGCGTTAG GAGAGAAGAG CAACCGGTGA AATCACTTGG GGACCATGAA 
TGGAACCGAA CACAGCAGAT TGGAGTCCTT AGCAGCCATG CATTTGAGAG TGATACTGAA 
ATGTCCGACA TAGATGATGA TGACCGTGAA ACAATTTTTA GTTCCATGGA CCTCTTATCT 
CCAGGTGGTC ACTCTGATGC TCAAACTTTA GCCATGATGC TTCAAGAACA GCTAGATGCC 
ATCAATAAAG AAATAAGACT CATTCAGGAA GAAAAGGAAT CTACTGAACT TCGTGCAGAA 
GAAATTGAAA ATAGAGTAGC CAGTGTTAGC CTAGAGGGGC TCAACCTGGC CAGAATGCAC 
CCAGGTACAT CGATCACTGC TTCAGTCACT GCTTCATCAT TAGCAAGTTC ATCACCTCCA 
AGTGGTCATT CTACACCCAA ACTCACACCT CGTAGTCCAG CAAGAGAAAT GGATCGAATG 
GGGGTCATGA CACTACCAAG TGATTTGAGA AAGCATCGAA GAAAGATTGC AGTTGTAGAA 
GAAGATGGGC GTGAAGATAA AGCCACAATA AAATGCGAAA CATCCCCTCC TTCAACACCA 
CGTGCTCTCA GGATGACACA TACCTTGCCA TCTTCATACC ATAATGATGC CAGAGGAAGT 
CTGACTGCTT CTCTTGAACC TGATGTGTTT GGTCTTGGGA GCACAAACAG TAGCCAAGAC 
TCTCTGCACA AAGCTCCAAA GAAGAAAGGA ATCAAGTCAT CCATTGGCCG GTTGTTTGGT 
AAAAAAGAGA AGGCTCGATT CGGACAGCTC AGTAAGGAAT TTGGTCTACA AGGCGCATTT 
ATGGATGCTG ATCAAGCGGC ACAAGATTCC TTGGGCTTAG GAAAACTTGG AACTCAAGCA 
GAAAAAGACA GAAGGCTCAA AAAGAAACAT GAACTTCTGG AAGAAGCAAG AAGAAAGGGA 
CTTCCATTTG CACAGTGGGA TGGGCCAACC GTAGTTGCAT GGCTTGAACT TTGGTTGGGA 
ATGCCAGCTT GGTATGTTGC TGCATGTCGT GCCAACGTAA AGAGTGGTGC CATAATGTCT 
GCCTTGTCTG ATACGGAAAT ACAAAGGGAG ATTGGGATTA GCAATCCTCT GCATCGTTTA 
AAGCTACGGT TAGCAATCCA AGAGATGGTT TCACTCACCA GCCCATCAGC GCCTCCAACA 
TCCAGAACAC CATCAGGTAA TGTCTGGGTA ACCCATGAGG AAATGGAGAA CCTTACTGCT 
CCCACCAAAA CGAAAGAGTC AGAAGAAGGC AGCTGGGCAC AAACATTGGC ATATGGCGAT 
ATGAACCATG AGTGGATTGG GAATGAATGG CTTCCTAGTC TGGGCTTACC GCAGTACAGA 
AGTTACTTCA TGGAATGCCT TGTAGATGCC CGGATGCTAG ACCATTTAAC AAAAAAAGAT 
CTTCGAGTGC ACTTAAAAAT GGTGGATAGC TTTCATCGAA CAAGTTTGCA GTATGGCATA 
ATGTGCTTGA AAAAATTAAA CTATGATAGG AAGGAGCTGG AAAGGAGAAG AGAAACAAGC 
CAGCATGAAA TTAAAGATGT GTTAGTGTGG AGCAATGATC GTGTGATTCG CTGGATCCAA 
GCAATAGGCC TTAGAGATTA TGCAAACAAC ATACTGGAGA GTGGAATCCA CGGGTCACTT 
ATTGCCTTGG ATGAGAACTT TGACTACACA AGTTTAGCTC TTTTGTTACA AATCCCAACA 
CAAAATACTC AGGCACGGCA GATCCTGGAA AGAGAATACA ATAATTTATT GGCTTTGGGA 
ACTGAAAGGC GCCTTGATGA GAGTGATGAC AAGAACTTCA GACGTGGGCC CTCCTGGAGG 
AGGCAGTTTC CACCACGTGA AGTGCACGGA ATCAGCATGA TGCCAGGTTC TTCGGAAACA 
CTGCCAGCAG GATTCAGATT AACAACAACA TCCGGGCATT CACGGAAAAT GACAACAGAT 
GTTGCCCCTT CAGGAATACA GAGATTAGAC AGCTCCACGG TCCGTACATA CTCATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754748.1
CDS	322..4041
Translation	MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLGLAQQRLQDVIYDRDSLQRQLNSALPQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELTAANQEIVALREQNAHIQRKMGTGEGPEQEALEGMEQGQKIHDKRLSNGSIDSNDDASQMVELQELLEKQNYEMTQMKERLSSLSARVGEVEQEAESARKELVKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQREATSIHDINDKLENELANKEAILRQMEEKNRQLQERLEMAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEDKNILLQESETFRKQLEECLHDKDRLQEEVEKLRSEVDQVKIRTGSLIEPTLTRPHLDSSAELRYSVGSLVDSQSDYRSTKVIRRPRRGRMGVRREEQPVKSLGDHEWNRTQQIGVLSSHAFESDTEMSDIDDDDRETIFSSMDLLSPGGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPSTPRALRMTHTLPSSYHNDARGSLTASLEPDVFGLGSTNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARFGQLSKEFGLQGAFMDADQAAQDSLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLTAPTKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRKELERRRETSQHEIKDVLVWSNDRVIRWIQAIGLRDYANNILESGIHGSLIALDENFDYTSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGPSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGHSRKMTTDVAPSGIQRLDSSTVRTYSC

Target ORF information:

RefSeq Version	XM_031898888.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898888.1

ORF Insert Sequence:

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAGCTCGAC AACGGGAAAA AATGAATGAA 
GAGCACAACA AAAGATTGTC TGATACAGTG GACCGGCTTT TAACAGAGTC CAATGAACGA 
CTCCAGTTAC ACTTGAAAGA ACGAATGGCT GCACTAGAAG ACAAGAATAT TTTACTTCAG 
GAATCAGAGA CCTTCAGAAA ACAGCTAGAA GAATGCTTGC ATGATAAGGA TCGGCTACAA 
GAAGAAGTTG AAAAATTAAG ATCTGAGGTT GATCAAGTGA AGATAAGAAC AGGGTCCTTG 
ATAGAACCTA CATTAACGAG GCCTCACCTT GACTCATCTG CAGAGTTAAG ATATTCAGTA 
GGGTCTCTTG TTGACAGCCA GTCTGACTAC CGCTCAACTA AAGTCATACG AAGACCAAGA 
AGAGGTAGAA TGGGCGTTAG GAGAGAAGAG CAACCGGTGA AATCACTTGG GGACCATGAA 
TGGAACCGAA CACAGCAGAT TGGAGTCCTT AGCAGCCATG CATTTGAGAG TGATACTGAA 
ATGTCCGACA TAGATGATGA TGACCGTGAA ACAATTTTTA GTTCCATGGA CCTCTTATCT 
CCAGGTGGTC ACTCTGATGC TCAAACTTTA GCCATGATGC TTCAAGAACA GCTAGATGCC 
ATCAATAAAG AAATAAGACT CATTCAGGAA GAAAAGGAAT CTACTGAACT TCGTGCAGAA 
GAAATTGAAA ATAGAGTAGC CAGTGTTAGC CTAGAGGGGC TCAACCTGGC CAGAATGCAC 
CCAGGTACAT CGATCACTGC TTCAGTCACT GCTTCATCAT TAGCAAGTTC ATCACCTCCA 
AGTGGTCATT CTACACCCAA ACTCACACCT CGTAGTCCAG CAAGAGAAAT GGATCGAATG 
GGGGTCATGA CACTACCAAG TGATTTGAGA AAGCATCGAA GAAAGATTGC AGTTGTAGAA 
GAAGATGGGC GTGAAGATAA AGCCACAATA AAATGCGAAA CATCCCCTCC TTCAACACCA 
CGTGCTCTCA GGATGACACA TACCTTGCCA TCTTCATACC ATAATGATGC CAGAGGAAGT 
CTGACTGCTT CTCTTGAACC TGATGTGTTT GGTCTTGGGA GCACAAACAG TAGCCAAGAC 
TCTCTGCACA AAGCTCCAAA GAAGAAAGGA ATCAAGTCAT CCATTGGCCG GTTGTTTGGT 
AAAAAAGAGA AGGCTCGATT CGGACAGCTC AGTAAGGAAT TTGGTCTACA AGGCGCATTT 
ATGGATGCTG ATCAAGCGGC ACAAGATTCC TTGGGCTTAG GAAAACTTGG AACTCAAGCA 
GAAAAAGACA GAAGGCTCAA AAAGAAACAT GAACTTCTGG AAGAAGCAAG AAGAAAGGGA 
CTTCCATTTG CACAGTGGGA TGGGCCAACC GTAGTTGCAT GGCTTGAACT TTGGTTGGGA 
ATGCCAGCTT GGTATGTTGC TGCATGTCGT GCCAACGTAA AGAGTGGTGC CATAATGTCT 
GCCTTGTCTG ATACGGAAAT ACAAAGGGAG ATTGGGATTA GCAATCCTCT GCATCGTTTA 
AAGCTACGGT TAGCAATCCA AGAGATGGTT TCACTCACCA GCCCATCAGC GCCTCCAACA 
TCCAGAACAC CATCAGGTAA TGTCTGGGTA ACCCATGAGG AAATGGAGAA CCTTACTGCT 
CCCACCAAAA CGAAAGAGTC AGAAGAAGGC AGCTGGGCAC AAACATTGGC ATATGGCGAT 
ATGAACCATG AGTGGATTGG GAATGAATGG CTTCCTAGTC TGGGCTTACC GCAGTACAGA 
AGTTACTTCA TGGAATGCCT TGTAGATGCC CGGATGCTAG ACCATTTAAC AAAAAAAGAT 
CTTCGAGTGC ACTTAAAAAT GGTGGATAGC TTTCATCGAA CAAGTTTGCA GTATGGCATA 
ATGTGCTTGA AAAAATTAAA CTATGATAGG AAGGAGCTGG AAAGGAGAAG AGAAACAAGC 
CAGCATGAAA TTAAAGATGT GTTAGTGTGG AGCAATGATC GTGTGATTCG CTGGATCCAA 
GCAATAGGCC TTAGAGATTA TGCAAACAAC ATACTGGAGA GTGGAATCCA CGGGTCACTT 
ATTGCCTTGG ATGAGAACTT TGACTACACA AGTTTAGCTC TTTTGTTACA AATCCCAACA 
CAAAATACTC AGGCACGGCA GATCCTGGAA AGAGAATACA ATAATTTATT GGCTTTGGGA 
ACTGAAAGGC GCCTTGATGA GAGTGATGAC AAGAACTTCA GACGTGGGCC CTCCTGGAGG 
AGGCAGTTTC CACCACGTGA AGTGCACGGA ATCAGCATGA TGCCAGGTTC TTCGGAAACA 
CTGCCAGCAG GATTCAGATT AACAACAACA TCCGGGCATT CACGGAAAAT GACAACAGAT 
GTTGCCCCTT CAGGAATACA GAGATTAGAC AGCTCCACGG TCCGTACATA CTCATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia2 ( XM_031898889.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754749.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X3, mRNA.

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CloneID	OXa54660
Clone ID Related Accession (Same CDS sequence)	XM_031898889.1
Accession Version	XM_031898889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	liprin-alpha-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAGCTCGAC AACGGGAAAA AATGAATGAA 
GAGCACAACA AAAGATTGTC TGATACAGTG GACCGGCTTT TAACAGAGTC CAATGAACGA 
CTCCAGTTAC ACTTGAAAGA ACGAATGGCT GCACTAGAAG ACAAGAATAT TTTACTTCAG 
GAATCAGAGA CCTTCAGAAA ACAGCTAGAA GAATGCTTGC ATGATAAGGA TCGGCTACAA 
GAAGAAGTTG AAAAATTAAG ATCTGAGGTT GATCAAGTGA AGATAAGAAC AGGGTCCTTG 
ATAGAACCTA CATTAACGAG GCCTCACCTT GACTCATCTG CAGAGTTAAG ATATTCAGTA 
GGGTCTCTTG TTGACAGCCA GTCTGACTAC CGCTCAACTA AAGTCATACG AAGACCAAGA 
AGAGGTAGAA TGGGCGTTAG GAGAGAAGAG CAACCGGTGA AATCACTTGG GGACCATGAA 
TGGAACCGAA CACAGCAGAT TGGAGTCCTT AGCAGCCATG CATTTGAGAG TGATACTGAA 
ATGTCCGACA TAGATGATGA TGACCGTGAA ACAATTTTTA GTTCCATGGA CCTCTTATCT 
CCAGGTGGTC ACTCTGATGC TCAAACTTTA GCCATGATGC TTCAAGAACA GCTAGATGCC 
ATCAATAAAG AAATAAGACT CATTCAGGAA GAAAAGGAAT CTACTGAACT TCGTGCAGAA 
GAAATTGAAA ATAGAGTAGC CAGTGTTAGC CTAGAGGGGC TCAACCTGGC CAGAATGCAC 
CCAGGTACAT CGATCACTGC TTCAGTCACT GCTTCATCAT TAGCAAGTTC ATCACCTCCA 
AGTGGTCATT CTACACCCAA ACTCACACCT CGTAGTCCAG CAAGAGAAAT GGATCGAATG 
GGGGTCATGA CACTACCAAG TGATTTGAGA AAGCATCGAA GAAAGATTGC AGTTGTAGAA 
GAAGATGGGC GTGAAGATAA AGCCACAATA AAATGCGAAA CATCCCCTCC TTCAACACCA 
CGTGCTCTCA GGATGACACA TACCTTGCCA TCTTCATACC ATAATGATGC CAGAGGAAGT 
CTGACTGCTT CTCTTGAACC TGATGTGTTT GGTCTTGGGA GCACAAACAG TAGCCAAGAC 
TCTCTGCACA AAGCTCCAAA GAAGAAAGGA ATCAAGTCAT CCATTGGCCG GTTGTTTGGT 
AAAAAAGAGA AGGCTCGATT CGGACAGCTC AGCGCATTTA TGGATGCTGA TCAAGCGGCA 
CAAGATTCCT TGGGCTTAGG AAAACTTGGA ACTCAAGCAG AAAAAGACAG AAGGCTCAAA 
AAGAAACATG AACTTCTGGA AGAAGCAAGA AGAAAGGGAC TTCCATTTGC ACAGTGGGAT 
GGGCCAACCG TAGTTGCATG GCTTGAACTT TGGTTGGGAA TGCCAGCTTG GTATGTTGCT 
GCATGTCGTG CCAACGTAAA GAGTGGTGCC ATAATGTCTG CCTTGTCTGA TACGGAAATA 
CAAAGGGAGA TTGGGATTAG CAATCCTCTG CATCGTTTAA AGCTACGGTT AGCAATCCAA 
GAGATGGTTT CACTCACCAG CCCATCAGCG CCTCCAACAT CCAGAACACC ATCAGGTAAT 
GTCTGGGTAA CCCATGAGGA AATGGAGAAC CTTACTGCTC CCACCAAAAC GAAAGAGTCA 
GAAGAAGGCA GCTGGGCACA AACATTGGCA TATGGCGATA TGAACCATGA GTGGATTGGG 
AATGAATGGC TTCCTAGTCT GGGCTTACCG CAGTACAGAA GTTACTTCAT GGAATGCCTT 
GTAGATGCCC GGATGCTAGA CCATTTAACA AAAAAAGATC TTCGAGTGCA CTTAAAAATG 
GTGGATAGCT TTCATCGAAC AAGTTTGCAG TATGGCATAA TGTGCTTGAA AAAATTAAAC 
TATGATAGGA AGGAGCTGGA AAGGAGAAGA GAAACAAGCC AGCATGAAAT TAAAGATGTG 
TTAGTGTGGA GCAATGATCG TGTGATTCGC TGGATCCAAG CAATAGGCCT TAGAGATTAT 
GCAAACAACA TACTGGAGAG TGGAATCCAC GGGTCACTTA TTGCCTTGGA TGAGAACTTT 
GACTACACAA GTTTAGCTCT TTTGTTACAA ATCCCAACAC AAAATACTCA GGCACGGCAG 
ATCCTGGAAA GAGAATACAA TAATTTATTG GCTTTGGGAA CTGAAAGGCG CCTTGATGAG 
AGTGATGACA AGAACTTCAG ACGTGGGCCC TCCTGGAGGA GGCAGTTTCC ACCACGTGAA 
GTGCACGGAA TCAGCATGAT GCCAGGTTCT TCGGAAACAC TGCCAGCAGG ATTCAGATTA 
ACAACAACAT CCGGGCATTC ACGGAAAATG ACAACAGATG TTGCCCCTTC AGGAATACAG 
AGATTAGACA GCTCCACGGT CCGTACATAC TCATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754749.1
CDS	321..4019
Translation	MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLGLAQQRLQDVIYDRDSLQRQLNSALPQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELTAANQEIVALREQNAHIQRKMGTGEGPEQEALEGMEQGQKIHDKRLSNGSIDSNDDASQMVELQELLEKQNYEMTQMKERLSSLSARVGEVEQEAESARKELVKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQREATSIHDINDKLENELANKEAILRQMEEKNRQLQERLEMAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEDKNILLQESETFRKQLEECLHDKDRLQEEVEKLRSEVDQVKIRTGSLIEPTLTRPHLDSSAELRYSVGSLVDSQSDYRSTKVIRRPRRGRMGVRREEQPVKSLGDHEWNRTQQIGVLSSHAFESDTEMSDIDDDDRETIFSSMDLLSPGGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPSTPRALRMTHTLPSSYHNDARGSLTASLEPDVFGLGSTNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARFGQLSAFMDADQAAQDSLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLTAPTKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRKELERRRETSQHEIKDVLVWSNDRVIRWIQAIGLRDYANNILESGIHGSLIALDENFDYTSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGPSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGHSRKMTTDVAPSGIQRLDSSTVRTYSC

Target ORF information:

RefSeq Version	XM_031898889.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898889.1

ORF Insert Sequence:

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAGCTCGAC AACGGGAAAA AATGAATGAA 
GAGCACAACA AAAGATTGTC TGATACAGTG GACCGGCTTT TAACAGAGTC CAATGAACGA 
CTCCAGTTAC ACTTGAAAGA ACGAATGGCT GCACTAGAAG ACAAGAATAT TTTACTTCAG 
GAATCAGAGA CCTTCAGAAA ACAGCTAGAA GAATGCTTGC ATGATAAGGA TCGGCTACAA 
GAAGAAGTTG AAAAATTAAG ATCTGAGGTT GATCAAGTGA AGATAAGAAC AGGGTCCTTG 
ATAGAACCTA CATTAACGAG GCCTCACCTT GACTCATCTG CAGAGTTAAG ATATTCAGTA 
GGGTCTCTTG TTGACAGCCA GTCTGACTAC CGCTCAACTA AAGTCATACG AAGACCAAGA 
AGAGGTAGAA TGGGCGTTAG GAGAGAAGAG CAACCGGTGA AATCACTTGG GGACCATGAA 
TGGAACCGAA CACAGCAGAT TGGAGTCCTT AGCAGCCATG CATTTGAGAG TGATACTGAA 
ATGTCCGACA TAGATGATGA TGACCGTGAA ACAATTTTTA GTTCCATGGA CCTCTTATCT 
CCAGGTGGTC ACTCTGATGC TCAAACTTTA GCCATGATGC TTCAAGAACA GCTAGATGCC 
ATCAATAAAG AAATAAGACT CATTCAGGAA GAAAAGGAAT CTACTGAACT TCGTGCAGAA 
GAAATTGAAA ATAGAGTAGC CAGTGTTAGC CTAGAGGGGC TCAACCTGGC CAGAATGCAC 
CCAGGTACAT CGATCACTGC TTCAGTCACT GCTTCATCAT TAGCAAGTTC ATCACCTCCA 
AGTGGTCATT CTACACCCAA ACTCACACCT CGTAGTCCAG CAAGAGAAAT GGATCGAATG 
GGGGTCATGA CACTACCAAG TGATTTGAGA AAGCATCGAA GAAAGATTGC AGTTGTAGAA 
GAAGATGGGC GTGAAGATAA AGCCACAATA AAATGCGAAA CATCCCCTCC TTCAACACCA 
CGTGCTCTCA GGATGACACA TACCTTGCCA TCTTCATACC ATAATGATGC CAGAGGAAGT 
CTGACTGCTT CTCTTGAACC TGATGTGTTT GGTCTTGGGA GCACAAACAG TAGCCAAGAC 
TCTCTGCACA AAGCTCCAAA GAAGAAAGGA ATCAAGTCAT CCATTGGCCG GTTGTTTGGT 
AAAAAAGAGA AGGCTCGATT CGGACAGCTC AGCGCATTTA TGGATGCTGA TCAAGCGGCA 
CAAGATTCCT TGGGCTTAGG AAAACTTGGA ACTCAAGCAG AAAAAGACAG AAGGCTCAAA 
AAGAAACATG AACTTCTGGA AGAAGCAAGA AGAAAGGGAC TTCCATTTGC ACAGTGGGAT 
GGGCCAACCG TAGTTGCATG GCTTGAACTT TGGTTGGGAA TGCCAGCTTG GTATGTTGCT 
GCATGTCGTG CCAACGTAAA GAGTGGTGCC ATAATGTCTG CCTTGTCTGA TACGGAAATA 
CAAAGGGAGA TTGGGATTAG CAATCCTCTG CATCGTTTAA AGCTACGGTT AGCAATCCAA 
GAGATGGTTT CACTCACCAG CCCATCAGCG CCTCCAACAT CCAGAACACC ATCAGGTAAT 
GTCTGGGTAA CCCATGAGGA AATGGAGAAC CTTACTGCTC CCACCAAAAC GAAAGAGTCA 
GAAGAAGGCA GCTGGGCACA AACATTGGCA TATGGCGATA TGAACCATGA GTGGATTGGG 
AATGAATGGC TTCCTAGTCT GGGCTTACCG CAGTACAGAA GTTACTTCAT GGAATGCCTT 
GTAGATGCCC GGATGCTAGA CCATTTAACA AAAAAAGATC TTCGAGTGCA CTTAAAAATG 
GTGGATAGCT TTCATCGAAC AAGTTTGCAG TATGGCATAA TGTGCTTGAA AAAATTAAAC 
TATGATAGGA AGGAGCTGGA AAGGAGAAGA GAAACAAGCC AGCATGAAAT TAAAGATGTG 
TTAGTGTGGA GCAATGATCG TGTGATTCGC TGGATCCAAG CAATAGGCCT TAGAGATTAT 
GCAAACAACA TACTGGAGAG TGGAATCCAC GGGTCACTTA TTGCCTTGGA TGAGAACTTT 
GACTACACAA GTTTAGCTCT TTTGTTACAA ATCCCAACAC AAAATACTCA GGCACGGCAG 
ATCCTGGAAA GAGAATACAA TAATTTATTG GCTTTGGGAA CTGAAAGGCG CCTTGATGAG 
AGTGATGACA AGAACTTCAG ACGTGGGCCC TCCTGGAGGA GGCAGTTTCC ACCACGTGAA 
GTGCACGGAA TCAGCATGAT GCCAGGTTCT TCGGAAACAC TGCCAGCAGG ATTCAGATTA 
ACAACAACAT CCGGGCATTC ACGGAAAATG ACAACAGATG TTGCCCCTTC AGGAATACAG 
AGATTAGACA GCTCCACGGT CCGTACATAC TCATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia2 ( XM_031898890.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754750.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa54661
Clone ID Related Accession (Same CDS sequence)	XM_031898890.1
Accession Version	XM_031898890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	liprin-alpha-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGAT TTATAAAGGA GCTTAATTCT CATGCCTGCA TCTGTATCAC TTTGTGGATT 
GTATCGAATG TGAGTTCAGA AATGATTTTC TCGGATATGA ACACAGTTTC TGGGTCTCCA 
AAAGTGCATC TGCCAAATGG AACCAGGTAC TACACATTCC AGGAGTTTGC TGCATTGACC 
AAGGAACTGA ATGCATGCAG GGAGCAGCTT TTAGAAAAAG AAGAAGAGAT TTCTGAACTG 
AAAGCAGAAA GGAATAACAC CAGGCTTTTG TTGGAACATT TGGAGTGCCT TGTTTCCAGA 
CACGAAAGGT CACTGAGAAT GACAGTGGTA AAGCGGCAAG CACAGTCCCC CTCAGGAGTG 
TCTAGTGAAG TTGAAGTTCT AAAAGCATTA AAATCATTGT TTGAACATCA TAAAGCACTT 
GACGAGAAGG TAAGGGAACG GCTGAGGGTT TCTTTGGAAA GAGTCTCTGC ACTGGAAGAA 
GAGCTAACTG CTGCAAACCA GGAGATTGTT GCATTAAGAG AACAAAATGC CCACATACAA 
AGAAAGATGG GTACTGGAGA AGGGCCTGAG CAGGAAGCTC TTGAAGGAAT GGAGCAAGGA 
CAAAAGATTC ATGATAAGAG ACTGTCCAAT GGATCGATAG ATTCAAATGA TGATGCAAGT 
CAGATGGTGG AGCTGCAGGA ACTGCTTGAG AAACAAAACT ATGAAATGAC ACAAATGAAA 
GAGCGATTAT CATCATTGTC AGCCCGTGTT GGGGAGGTCG AACAAGAAGC AGAGTCAGCA 
AGAAAAGAAC TTGTGAAAAC TGAAGAGATG AATACTAAAT ACCAAAGGGA TATCAGGGAG 
GCTATGGCAC AAAAGGAGGA TATGGAAGAA AGAATCACTA CTTTAGAGAA ACGCTATCTC 
AGTGCACAGA GAGAGGCAAC ATCCATACAT GACATAAATG ACAAGTTGGA AAATGAACTG 
GCAAATAAGG AAGCAATTTT ACGTCAGATG GAAGAGAAAA ACAGGCAATT ACAAGAGCGG 
CTGGAAATGG CTGAGCAGAA GTTGCAACAG ACTATGAGAA AAGCTGAGAC CTTACCAGAA 
GTCGAAGCTG AGTTAGCGCA AAGAATTGCT GCACTTACAA AGGCAGAAGA AAGGCATGGG 
AATATTGAAG AAAGGATGAG GCACCTAGAA GCACAGCTTG AAGAGAAGAA CCAAGAACTT 
CAAAGAGCTC GACAACGGGA AAAAATGAAT GAAGAGCACA ACAAAAGATT GTCTGATACA 
GTGGACCGGC TTTTAACAGA GTCCAATGAA CGACTCCAGT TACACTTGAA AGAACGAATG 
GCTGCACTAG AAGACAAGAA TATTTTACTT CAGGAATCAG AGACCTTCAG AAAACAGCTA 
GAAGAATGCT TGCATGATAA GGATCGGCTA CAAGAAGAAG TTGAAAAATT AAGATCTGAG 
GTTGATCAAG TGAAGATAAG AACAGGGTCC TTGATAGAAC CTACATTAAC GAGGCCTCAC 
CTTGACTCAT CTGCAGAGTT AAGATATTCA GTAGGGTCTC TTGTTGACAG CCAGTCTGAC 
TACCGCTCAA CTAAAGTCAT ACGAAGACCA AGAAGAGGTA GAATGGGCGT TAGGAGAGAA 
GAGCAACCGG TGAAATCACT TGGGGACCAT GAATGGAACC GAACACAGCA GATTGGAGTC 
CTTAGCAGCC ATGCATTTGA GAGTGATACT GAAATGTCCG ACATAGATGA TGATGACCGT 
GAAACAATTT TTAGTTCCAT GGACCTCTTA TCTCCAGGTG GTCACTCTGA TGCTCAAACT 
TTAGCCATGA TGCTTCAAGA ACAGCTAGAT GCCATCAATA AAGAAATAAG ACTCATTCAG 
GAAGAAAAGG AATCTACTGA ACTTCGTGCA GAAGAAATTG AAAATAGAGT AGCCAGTGTT 
AGCCTAGAGG GGCTCAACCT GGCCAGAATG CACCCAGGTA CATCGATCAC TGCTTCAGTC 
ACTGCTTCAT CATTAGCAAG TTCATCACCT CCAAGTGGTC ATTCTACACC CAAACTCACA 
CCTCGTAGTC CAGCAAGAGA AATGGATCGA ATGGGGGTCA TGACACTACC AAGTGATTTG 
AGAAAGCATC GAAGAAAGAT TGCAGTTGTA GAAGAAGATG GGCGTGAAGA TAAAGCCACA 
ATAAAATGCG AAACATCCCC TCCTTCAACA CCACGTGCTC TCAGGATGAC ACATACCTTG 
CCATCTTCAT ACCATAATGA TGCCAGAGGA AGTCTGACTG CTTCTCTTGA ACCTGATGTG 
TTTGGTCTTG GGAGCACAAA CAGTAGCCAA GACTCTCTGC ACAAAGCTCC AAAGAAGAAA 
GGAATCAAGT CATCCATTGG CCGGTTGTTT GGTAAAAAAG AGAAGGCTCG ATTCGGACAG 
CTCAGTAAGG AATTTGGTCT ACAAGGTCAA GGCGCATTTA TGGATGCTGA TCAAGCGGCA 
CAAGATTCCT TGGGCTTAGG AAAACTTGGA ACTCAAGCAG AAAAAGACAG AAGGCTCAAA 
AAGAAACATG AACTTCTGGA AGAAGCAAGA AGAAAGGGAC TTCCATTTGC ACAGTGGGAT 
GGGCCAACCG TAGTTGCATG GCTTGAACTT TGGTTGGGAA TGCCAGCTTG GTATGTTGCT 
GCATGTCGTG CCAACGTAAA GAGTGGTGCC ATAATGTCTG CCTTGTCTGA TACGGAAATA 
CAAAGGGAGA TTGGGATTAG CAATCCTCTG CATCGTTTAA AGCTACGGTT AGCAATCCAA 
GAGATGGTTT CACTCACCAG CCCATCAGCG CCTCCAACAT CCAGAACACC ATCAGGTAAT 
GTCTGGGTAA CCCATGAGGA AATGGAGAAC CTTACTGCTC CCACCAAAAC GAAAGAGTCA 
GAAGAAGGCA GCTGGGCACA AACATTGGCA TATGGCGATA TGAACCATGA GTGGATTGGG 
AATGAATGGC TTCCTAGTCT GGGCTTACCG CAGTACAGAA GTTACTTCAT GGAATGCCTT 
GTAGATGCCC GGATGCTAGA CCATTTAACA AAAAAAGATC TTCGAGTGCA CTTAAAAATG 
GTGGATAGCT TTCATCGAAC AAGTTTGCAG TATGGCATAA TGTGCTTGAA AAAATTAAAC 
TATGATAGGA AGGAGCTGGA AAGGAGAAGA GAAACAAGCC AGCATGAAAT TAAAGATGTG 
TTAGTGTGGA GCAATGATCG TGTGATTCGC TGGATCCAAG CAATAGGCCT TAGAGATTAT 
GCAAACAACA TACTGGAGAG TGGAATCCAC GGGTCACTTA TTGCCTTGGA TGAGAACTTT 
GACTACACAA GTTTAGCTCT TTTGTTACAA ATCCCAACAC AAAATACTCA GGCACGGCAG 
ATCCTGGAAA GAGAATACAA TAATTTATTG GCTTTGGGAA CTGAAAGGCG CCTTGATGAG 
AGTGATGACA AGAACTTCAG ACGTGGGCCC TCCTGGAGGA GGCAGTTTCC ACCACGTGAA 
GTGCACGGAA TCAGCATGAT GCCAGGTTCT TCGGAAACAC TGCCAGCAGG ATTCAGATTA 
ACAACAACAT CCGGGCATTC ACGGAAAATG ACAACAGATG TTGCCCCTTC AGGAATACAG 
AGATTAGACA GCTCCACGGT CCGTACATAC TCATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754750.1
CDS	245..3883
Translation	MNGFIKELNSHACICITLWIVSNVSSEMIFSDMNTVSGSPKVHLPNGTRYYTFQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELTAANQEIVALREQNAHIQRKMGTGEGPEQEALEGMEQGQKIHDKRLSNGSIDSNDDASQMVELQELLEKQNYEMTQMKERLSSLSARVGEVEQEAESARKELVKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQREATSIHDINDKLENELANKEAILRQMEEKNRQLQERLEMAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEDKNILLQESETFRKQLEECLHDKDRLQEEVEKLRSEVDQVKIRTGSLIEPTLTRPHLDSSAELRYSVGSLVDSQSDYRSTKVIRRPRRGRMGVRREEQPVKSLGDHEWNRTQQIGVLSSHAFESDTEMSDIDDDDRETIFSSMDLLSPGGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPSTPRALRMTHTLPSSYHNDARGSLTASLEPDVFGLGSTNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARFGQLSKEFGLQGQGAFMDADQAAQDSLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLTAPTKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRKELERRRETSQHEIKDVLVWSNDRVIRWIQAIGLRDYANNILESGIHGSLIALDENFDYTSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGPSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGHSRKMTTDVAPSGIQRLDSSTVRTYSC

Target ORF information:

RefSeq Version	XM_031898890.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898890.1

ORF Insert Sequence:

ATGAACGGAT TTATAAAGGA GCTTAATTCT CATGCCTGCA TCTGTATCAC TTTGTGGATT 
GTATCGAATG TGAGTTCAGA AATGATTTTC TCGGATATGA ACACAGTTTC TGGGTCTCCA 
AAAGTGCATC TGCCAAATGG AACCAGGTAC TACACATTCC AGGAGTTTGC TGCATTGACC 
AAGGAACTGA ATGCATGCAG GGAGCAGCTT TTAGAAAAAG AAGAAGAGAT TTCTGAACTG 
AAAGCAGAAA GGAATAACAC CAGGCTTTTG TTGGAACATT TGGAGTGCCT TGTTTCCAGA 
CACGAAAGGT CACTGAGAAT GACAGTGGTA AAGCGGCAAG CACAGTCCCC CTCAGGAGTG 
TCTAGTGAAG TTGAAGTTCT AAAAGCATTA AAATCATTGT TTGAACATCA TAAAGCACTT 
GACGAGAAGG TAAGGGAACG GCTGAGGGTT TCTTTGGAAA GAGTCTCTGC ACTGGAAGAA 
GAGCTAACTG CTGCAAACCA GGAGATTGTT GCATTAAGAG AACAAAATGC CCACATACAA 
AGAAAGATGG GTACTGGAGA AGGGCCTGAG CAGGAAGCTC TTGAAGGAAT GGAGCAAGGA 
CAAAAGATTC ATGATAAGAG ACTGTCCAAT GGATCGATAG ATTCAAATGA TGATGCAAGT 
CAGATGGTGG AGCTGCAGGA ACTGCTTGAG AAACAAAACT ATGAAATGAC ACAAATGAAA 
GAGCGATTAT CATCATTGTC AGCCCGTGTT GGGGAGGTCG AACAAGAAGC AGAGTCAGCA 
AGAAAAGAAC TTGTGAAAAC TGAAGAGATG AATACTAAAT ACCAAAGGGA TATCAGGGAG 
GCTATGGCAC AAAAGGAGGA TATGGAAGAA AGAATCACTA CTTTAGAGAA ACGCTATCTC 
AGTGCACAGA GAGAGGCAAC ATCCATACAT GACATAAATG ACAAGTTGGA AAATGAACTG 
GCAAATAAGG AAGCAATTTT ACGTCAGATG GAAGAGAAAA ACAGGCAATT ACAAGAGCGG 
CTGGAAATGG CTGAGCAGAA GTTGCAACAG ACTATGAGAA AAGCTGAGAC CTTACCAGAA 
GTCGAAGCTG AGTTAGCGCA AAGAATTGCT GCACTTACAA AGGCAGAAGA AAGGCATGGG 
AATATTGAAG AAAGGATGAG GCACCTAGAA GCACAGCTTG AAGAGAAGAA CCAAGAACTT 
CAAAGAGCTC GACAACGGGA AAAAATGAAT GAAGAGCACA ACAAAAGATT GTCTGATACA 
GTGGACCGGC TTTTAACAGA GTCCAATGAA CGACTCCAGT TACACTTGAA AGAACGAATG 
GCTGCACTAG AAGACAAGAA TATTTTACTT CAGGAATCAG AGACCTTCAG AAAACAGCTA 
GAAGAATGCT TGCATGATAA GGATCGGCTA CAAGAAGAAG TTGAAAAATT AAGATCTGAG 
GTTGATCAAG TGAAGATAAG AACAGGGTCC TTGATAGAAC CTACATTAAC GAGGCCTCAC 
CTTGACTCAT CTGCAGAGTT AAGATATTCA GTAGGGTCTC TTGTTGACAG CCAGTCTGAC 
TACCGCTCAA CTAAAGTCAT ACGAAGACCA AGAAGAGGTA GAATGGGCGT TAGGAGAGAA 
GAGCAACCGG TGAAATCACT TGGGGACCAT GAATGGAACC GAACACAGCA GATTGGAGTC 
CTTAGCAGCC ATGCATTTGA GAGTGATACT GAAATGTCCG ACATAGATGA TGATGACCGT 
GAAACAATTT TTAGTTCCAT GGACCTCTTA TCTCCAGGTG GTCACTCTGA TGCTCAAACT 
TTAGCCATGA TGCTTCAAGA ACAGCTAGAT GCCATCAATA AAGAAATAAG ACTCATTCAG 
GAAGAAAAGG AATCTACTGA ACTTCGTGCA GAAGAAATTG AAAATAGAGT AGCCAGTGTT 
AGCCTAGAGG GGCTCAACCT GGCCAGAATG CACCCAGGTA CATCGATCAC TGCTTCAGTC 
ACTGCTTCAT CATTAGCAAG TTCATCACCT CCAAGTGGTC ATTCTACACC CAAACTCACA 
CCTCGTAGTC CAGCAAGAGA AATGGATCGA ATGGGGGTCA TGACACTACC AAGTGATTTG 
AGAAAGCATC GAAGAAAGAT TGCAGTTGTA GAAGAAGATG GGCGTGAAGA TAAAGCCACA 
ATAAAATGCG AAACATCCCC TCCTTCAACA CCACGTGCTC TCAGGATGAC ACATACCTTG 
CCATCTTCAT ACCATAATGA TGCCAGAGGA AGTCTGACTG CTTCTCTTGA ACCTGATGTG 
TTTGGTCTTG GGAGCACAAA CAGTAGCCAA GACTCTCTGC ACAAAGCTCC AAAGAAGAAA 
GGAATCAAGT CATCCATTGG CCGGTTGTTT GGTAAAAAAG AGAAGGCTCG ATTCGGACAG 
CTCAGTAAGG AATTTGGTCT ACAAGGTCAA GGCGCATTTA TGGATGCTGA TCAAGCGGCA 
CAAGATTCCT TGGGCTTAGG AAAACTTGGA ACTCAAGCAG AAAAAGACAG AAGGCTCAAA 
AAGAAACATG AACTTCTGGA AGAAGCAAGA AGAAAGGGAC TTCCATTTGC ACAGTGGGAT 
GGGCCAACCG TAGTTGCATG GCTTGAACTT TGGTTGGGAA TGCCAGCTTG GTATGTTGCT 
GCATGTCGTG CCAACGTAAA GAGTGGTGCC ATAATGTCTG CCTTGTCTGA TACGGAAATA 
CAAAGGGAGA TTGGGATTAG CAATCCTCTG CATCGTTTAA AGCTACGGTT AGCAATCCAA 
GAGATGGTTT CACTCACCAG CCCATCAGCG CCTCCAACAT CCAGAACACC ATCAGGTAAT 
GTCTGGGTAA CCCATGAGGA AATGGAGAAC CTTACTGCTC CCACCAAAAC GAAAGAGTCA 
GAAGAAGGCA GCTGGGCACA AACATTGGCA TATGGCGATA TGAACCATGA GTGGATTGGG 
AATGAATGGC TTCCTAGTCT GGGCTTACCG CAGTACAGAA GTTACTTCAT GGAATGCCTT 
GTAGATGCCC GGATGCTAGA CCATTTAACA AAAAAAGATC TTCGAGTGCA CTTAAAAATG 
GTGGATAGCT TTCATCGAAC AAGTTTGCAG TATGGCATAA TGTGCTTGAA AAAATTAAAC 
TATGATAGGA AGGAGCTGGA AAGGAGAAGA GAAACAAGCC AGCATGAAAT TAAAGATGTG 
TTAGTGTGGA GCAATGATCG TGTGATTCGC TGGATCCAAG CAATAGGCCT TAGAGATTAT 
GCAAACAACA TACTGGAGAG TGGAATCCAC GGGTCACTTA TTGCCTTGGA TGAGAACTTT 
GACTACACAA GTTTAGCTCT TTTGTTACAA ATCCCAACAC AAAATACTCA GGCACGGCAG 
ATCCTGGAAA GAGAATACAA TAATTTATTG GCTTTGGGAA CTGAAAGGCG CCTTGATGAG 
AGTGATGACA AGAACTTCAG ACGTGGGCCC TCCTGGAGGA GGCAGTTTCC ACCACGTGAA 
GTGCACGGAA TCAGCATGAT GCCAGGTTCT TCGGAAACAC TGCCAGCAGG ATTCAGATTA 
ACAACAACAT CCGGGCATTC ACGGAAAATG ACAACAGATG TTGCCCCTTC AGGAATACAG 
AGATTAGACA GCTCCACGGT CCGTACATAC TCATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia2 ( XM_031898891.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754751.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa54662
Clone ID Related Accession (Same CDS sequence)	XM_031898891.1
Accession Version	XM_031898891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	liprin-alpha-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGGAT TTATAAAGGA GCTTAATTCT CATGCCTGCA TCTGTATCAC TTTGTGGATT 
GTATCGAATG TGAGTTCAGA AATGATTTTC TCGGATATGA ACACAGTTTC TGGGTCTCCA 
AAAGTGCATC TGCCAAATGG AACCAGGTAC TACACATTCC AGGAGTTTGC TGCATTGACC 
AAGGAACTGA ATGCATGCAG GGAGCAGCTT TTAGAAAAAG AAGAAGAGAT TTCTGAACTG 
AAAGCAGAAA GGAATAACAC CAGGCTTTTG TTGGAACATT TGGAGTGCCT TGTTTCCAGA 
CACGAAAGGT CACTGAGAAT GACAGTGGTA AAGCGGCAAG CACAGTCCCC CTCAGGAGTG 
TCTAGTGAAG TTGAAGTTCT AAAAGCATTA AAATCATTGT TTGAACATCA TAAAGCACTT 
GACGAGAAGG TAAGGGAACG GCTGAGGGTT TCTTTGGAAA GAGTCTCTGC ACTGGAAGAA 
GAGCTAACTG CTGCAAACCA GGAGATTGTT GCATTAAGAG AACAAAATGC CCACATACAA 
AGAAAGATGG GTACTGGAGA AGGGCCTGAG CAGGAAGCTC TTGAAGGAAT GGAGCAAGGA 
CAAAAGATTC ATGATAAGAG ACTGTCCAAT GGATCGATAG ATTCAAATGA TGATGCAAGT 
CAGATGGTGG AGCTGCAGGA ACTGCTTGAG AAACAAAACT ATGAAATGAC ACAAATGAAA 
GAGCGATTAT CATCATTGTC AGCCCGTGTT GGGGAGGTCG AACAAGAAGC AGAGTCAGCA 
AGAAAAGAAC TTGTGAAAAC TGAAGAGATG AATACTAAAT ACCAAAGGGA TATCAGGGAG 
GCTATGGCAC AAAAGGAGGA TATGGAAGAA AGAATCACTA CTTTAGAGAA ACGCTATCTC 
AGTGCACAGA GAGAGGCAAC ATCCATACAT GACATAAATG ACAAGTTGGA AAATGAACTG 
GCAAATAAGG AAGCAATTTT ACGTCAGATG GAAGAGAAAA ACAGGCAATT ACAAGAGCGG 
CTGGAAATGG CTGAGCAGAA GTTGCAACAG ACTATGAGAA AAGCTGAGAC CTTACCAGAA 
GTCGAAGCTG AGTTAGCGCA AAGAATTGCT GCACTTACAA AGGCAGAAGA AAGGCATGGG 
AATATTGAAG AAAGGATGAG GCACCTAGAA GCACAGCTTG AAGAGAAGAA CCAAGAACTT 
CAAAGAGCTC GACAACGGGA AAAAATGAAT GAAGAGCACA ACAAAAGATT GTCTGATACA 
GTGGACCGGC TTTTAACAGA GTCCAATGAA CGACTCCAGT TACACTTGAA AGAACGAATG 
GCTGCACTAG AAGACAAGAA TATTTTACTT CAGGAATCAG AGACCTTCAG AAAACAGCTA 
GAAGAATGCT TGCATGATAA GGATCGGCTA CAAGAAGAAG TTGAAAAATT AAGATCTGAG 
GTTGATCAAG TGAAGATAAG AACAGGGTCC TTGATAGAAC CTACATTAAC GAGGCCTCAC 
CTTGACTCAT CTGCAGAGTT AAGATATTCA GTAGGGTCTC TTGTTGACAG CCAGTCTGAC 
TACCGCTCAA CTAAAGTCAT ACGAAGACCA AGAAGAGGTA GAATGGGCGT TAGGAGAGAA 
GAGCAACCGG TGAAATCACT TGGGGACCAT GAATGGAACC GAACACAGCA GATTGGAGTC 
CTTAGCAGCC ATGCATTTGA GAGTGATACT GAAATGTCCG ACATAGATGA TGATGACCGT 
GAAACAATTT TTAGTTCCAT GGACCTCTTA TCTCCAGGTG GTCACTCTGA TGCTCAAACT 
TTAGCCATGA TGCTTCAAGA ACAGCTAGAT GCCATCAATA AAGAAATAAG ACTCATTCAG 
GAAGAAAAGG AATCTACTGA ACTTCGTGCA GAAGAAATTG AAAATAGAGT AGCCAGTGTT 
AGCCTAGAGG GGCTCAACCT GGCCAGAATG CACCCAGGTA CATCGATCAC TGCTTCAGTC 
ACTGCTTCAT CATTAGCAAG TTCATCACCT CCAAGTGGTC ATTCTACACC CAAACTCACA 
CCTCGTAGTC CAGCAAGAGA AATGGATCGA ATGGGGGTCA TGACACTACC AAGTGATTTG 
AGAAAGCATC GAAGAAAGAT TGCAGTTGTA GAAGAAGATG GGCGTGAAGA TAAAGCCACA 
ATAAAATGCG AAACATCCCC TCCTTCAACA CCACGTGCTC TCAGGATGAC ACATACCTTG 
CCATCTTCAT ACCATAATGA TGCCAGAGGA AGTCTGACTG CTTCTCTTGA ACCTGATGTG 
TTTGGTCTTG GGAGCACAAA CAGTAGCCAA GACTCTCTGC ACAAAGCTCC AAAGAAGAAA 
GGAATCAAGT CATCCATTGG CCGGTTGTTT GGTAAAAAAG AGAAGGCTCG ATTCGGACAG 
CTCAGCGCAT TTATGGATGC TGATCAAGCG GCACAAGATT CCTTGGGCTT AGGAAAACTT 
GGAACTCAAG CAGAAAAAGA CAGAAGGCTC AAAAAGAAAC ATGAACTTCT GGAAGAAGCA 
AGAAGAAAGG GACTTCCATT TGCACAGTGG GATGGGCCAA CCGTAGTTGC ATGGCTTGAA 
CTTTGGTTGG GAATGCCAGC TTGGTATGTT GCTGCATGTC GTGCCAACGT AAAGAGTGGT 
GCCATAATGT CTGCCTTGTC TGATACGGAA ATACAAAGGG AGATTGGGAT TAGCAATCCT 
CTGCATCGTT TAAAGCTACG GTTAGCAATC CAAGAGATGG TTTCACTCAC CAGCCCATCA 
GCGCCTCCAA CATCCAGAAC ACCATCAGGT AATGTCTGGG TAACCCATGA GGAAATGGAG 
AACCTTACTG CTCCCACCAA AACGAAAGAG TCAGAAGAAG GCAGCTGGGC ACAAACATTG 
GCATATGGCG ATATGAACCA TGAGTGGATT GGGAATGAAT GGCTTCCTAG TCTGGGCTTA 
CCGCAGTACA GAAGTTACTT CATGGAATGC CTTGTAGATG CCCGGATGCT AGACCATTTA 
ACAAAAAAAG ATCTTCGAGT GCACTTAAAA ATGGTGGATA GCTTTCATCG AACAAGTTTG 
CAGTATGGCA TAATGTGCTT GAAAAAATTA AACTATGATA GGAAGGAGCT GGAAAGGAGA 
AGAGAAACAA GCCAGCATGA AATTAAAGAT GTGTTAGTGT GGAGCAATGA TCGTGTGATT 
CGCTGGATCC AAGCAATAGG CCTTAGAGAT TATGCAAACA ACATACTGGA GAGTGGAATC 
CACGGGTCAC TTATTGCCTT GGATGAGAAC TTTGACTACA CAAGTTTAGC TCTTTTGTTA 
CAAATCCCAA CACAAAATAC TCAGGCACGG CAGATCCTGG AAAGAGAATA CAATAATTTA 
TTGGCTTTGG GAACTGAAAG GCGCCTTGAT GAGAGTGATG ACAAGAACTT CAGACGTGGG 
CCCTCCTGGA GGAGGCAGTT TCCACCACGT GAAGTGCACG GAATCAGCAT GATGCCAGGT 
TCTTCGGAAA CACTGCCAGC AGGATTCAGA TTAACAACAA CATCCGGGCA TTCACGGAAA 
ATGACAACAG ATGTTGCCCC TTCAGGAATA CAGAGATTAG ACAGCTCCAC GGTCCGTACA 
TACTCATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754751.1
CDS	244..3855
Translation	MNGFIKELNSHACICITLWIVSNVSSEMIFSDMNTVSGSPKVHLPNGTRYYTFQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELTAANQEIVALREQNAHIQRKMGTGEGPEQEALEGMEQGQKIHDKRLSNGSIDSNDDASQMVELQELLEKQNYEMTQMKERLSSLSARVGEVEQEAESARKELVKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQREATSIHDINDKLENELANKEAILRQMEEKNRQLQERLEMAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEAQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAALEDKNILLQESETFRKQLEECLHDKDRLQEEVEKLRSEVDQVKIRTGSLIEPTLTRPHLDSSAELRYSVGSLVDSQSDYRSTKVIRRPRRGRMGVRREEQPVKSLGDHEWNRTQQIGVLSSHAFESDTEMSDIDDDDRETIFSSMDLLSPGGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPSTPRALRMTHTLPSSYHNDARGSLTASLEPDVFGLGSTNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARFGQLSAFMDADQAAQDSLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLTAPTKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRKELERRRETSQHEIKDVLVWSNDRVIRWIQAIGLRDYANNILESGIHGSLIALDENFDYTSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGPSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGHSRKMTTDVAPSGIQRLDSSTVRTYSC

Target ORF information:

RefSeq Version	XM_031898891.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898891.1

ORF Insert Sequence:

ATGAACGGAT TTATAAAGGA GCTTAATTCT CATGCCTGCA TCTGTATCAC TTTGTGGATT 
GTATCGAATG TGAGTTCAGA AATGATTTTC TCGGATATGA ACACAGTTTC TGGGTCTCCA 
AAAGTGCATC TGCCAAATGG AACCAGGTAC TACACATTCC AGGAGTTTGC TGCATTGACC 
AAGGAACTGA ATGCATGCAG GGAGCAGCTT TTAGAAAAAG AAGAAGAGAT TTCTGAACTG 
AAAGCAGAAA GGAATAACAC CAGGCTTTTG TTGGAACATT TGGAGTGCCT TGTTTCCAGA 
CACGAAAGGT CACTGAGAAT GACAGTGGTA AAGCGGCAAG CACAGTCCCC CTCAGGAGTG 
TCTAGTGAAG TTGAAGTTCT AAAAGCATTA AAATCATTGT TTGAACATCA TAAAGCACTT 
GACGAGAAGG TAAGGGAACG GCTGAGGGTT TCTTTGGAAA GAGTCTCTGC ACTGGAAGAA 
GAGCTAACTG CTGCAAACCA GGAGATTGTT GCATTAAGAG AACAAAATGC CCACATACAA 
AGAAAGATGG GTACTGGAGA AGGGCCTGAG CAGGAAGCTC TTGAAGGAAT GGAGCAAGGA 
CAAAAGATTC ATGATAAGAG ACTGTCCAAT GGATCGATAG ATTCAAATGA TGATGCAAGT 
CAGATGGTGG AGCTGCAGGA ACTGCTTGAG AAACAAAACT ATGAAATGAC ACAAATGAAA 
GAGCGATTAT CATCATTGTC AGCCCGTGTT GGGGAGGTCG AACAAGAAGC AGAGTCAGCA 
AGAAAAGAAC TTGTGAAAAC TGAAGAGATG AATACTAAAT ACCAAAGGGA TATCAGGGAG 
GCTATGGCAC AAAAGGAGGA TATGGAAGAA AGAATCACTA CTTTAGAGAA ACGCTATCTC 
AGTGCACAGA GAGAGGCAAC ATCCATACAT GACATAAATG ACAAGTTGGA AAATGAACTG 
GCAAATAAGG AAGCAATTTT ACGTCAGATG GAAGAGAAAA ACAGGCAATT ACAAGAGCGG 
CTGGAAATGG CTGAGCAGAA GTTGCAACAG ACTATGAGAA AAGCTGAGAC CTTACCAGAA 
GTCGAAGCTG AGTTAGCGCA AAGAATTGCT GCACTTACAA AGGCAGAAGA AAGGCATGGG 
AATATTGAAG AAAGGATGAG GCACCTAGAA GCACAGCTTG AAGAGAAGAA CCAAGAACTT 
CAAAGAGCTC GACAACGGGA AAAAATGAAT GAAGAGCACA ACAAAAGATT GTCTGATACA 
GTGGACCGGC TTTTAACAGA GTCCAATGAA CGACTCCAGT TACACTTGAA AGAACGAATG 
GCTGCACTAG AAGACAAGAA TATTTTACTT CAGGAATCAG AGACCTTCAG AAAACAGCTA 
GAAGAATGCT TGCATGATAA GGATCGGCTA CAAGAAGAAG TTGAAAAATT AAGATCTGAG 
GTTGATCAAG TGAAGATAAG AACAGGGTCC TTGATAGAAC CTACATTAAC GAGGCCTCAC 
CTTGACTCAT CTGCAGAGTT AAGATATTCA GTAGGGTCTC TTGTTGACAG CCAGTCTGAC 
TACCGCTCAA CTAAAGTCAT ACGAAGACCA AGAAGAGGTA GAATGGGCGT TAGGAGAGAA 
GAGCAACCGG TGAAATCACT TGGGGACCAT GAATGGAACC GAACACAGCA GATTGGAGTC 
CTTAGCAGCC ATGCATTTGA GAGTGATACT GAAATGTCCG ACATAGATGA TGATGACCGT 
GAAACAATTT TTAGTTCCAT GGACCTCTTA TCTCCAGGTG GTCACTCTGA TGCTCAAACT 
TTAGCCATGA TGCTTCAAGA ACAGCTAGAT GCCATCAATA AAGAAATAAG ACTCATTCAG 
GAAGAAAAGG AATCTACTGA ACTTCGTGCA GAAGAAATTG AAAATAGAGT AGCCAGTGTT 
AGCCTAGAGG GGCTCAACCT GGCCAGAATG CACCCAGGTA CATCGATCAC TGCTTCAGTC 
ACTGCTTCAT CATTAGCAAG TTCATCACCT CCAAGTGGTC ATTCTACACC CAAACTCACA 
CCTCGTAGTC CAGCAAGAGA AATGGATCGA ATGGGGGTCA TGACACTACC AAGTGATTTG 
AGAAAGCATC GAAGAAAGAT TGCAGTTGTA GAAGAAGATG GGCGTGAAGA TAAAGCCACA 
ATAAAATGCG AAACATCCCC TCCTTCAACA CCACGTGCTC TCAGGATGAC ACATACCTTG 
CCATCTTCAT ACCATAATGA TGCCAGAGGA AGTCTGACTG CTTCTCTTGA ACCTGATGTG 
TTTGGTCTTG GGAGCACAAA CAGTAGCCAA GACTCTCTGC ACAAAGCTCC AAAGAAGAAA 
GGAATCAAGT CATCCATTGG CCGGTTGTTT GGTAAAAAAG AGAAGGCTCG ATTCGGACAG 
CTCAGCGCAT TTATGGATGC TGATCAAGCG GCACAAGATT CCTTGGGCTT AGGAAAACTT 
GGAACTCAAG CAGAAAAAGA CAGAAGGCTC AAAAAGAAAC ATGAACTTCT GGAAGAAGCA 
AGAAGAAAGG GACTTCCATT TGCACAGTGG GATGGGCCAA CCGTAGTTGC ATGGCTTGAA 
CTTTGGTTGG GAATGCCAGC TTGGTATGTT GCTGCATGTC GTGCCAACGT AAAGAGTGGT 
GCCATAATGT CTGCCTTGTC TGATACGGAA ATACAAAGGG AGATTGGGAT TAGCAATCCT 
CTGCATCGTT TAAAGCTACG GTTAGCAATC CAAGAGATGG TTTCACTCAC CAGCCCATCA 
GCGCCTCCAA CATCCAGAAC ACCATCAGGT AATGTCTGGG TAACCCATGA GGAAATGGAG 
AACCTTACTG CTCCCACCAA AACGAAAGAG TCAGAAGAAG GCAGCTGGGC ACAAACATTG 
GCATATGGCG ATATGAACCA TGAGTGGATT GGGAATGAAT GGCTTCCTAG TCTGGGCTTA 
CCGCAGTACA GAAGTTACTT CATGGAATGC CTTGTAGATG CCCGGATGCT AGACCATTTA 
ACAAAAAAAG ATCTTCGAGT GCACTTAAAA ATGGTGGATA GCTTTCATCG AACAAGTTTG 
CAGTATGGCA TAATGTGCTT GAAAAAATTA AACTATGATA GGAAGGAGCT GGAAAGGAGA 
AGAGAAACAA GCCAGCATGA AATTAAAGAT GTGTTAGTGT GGAGCAATGA TCGTGTGATT 
CGCTGGATCC AAGCAATAGG CCTTAGAGAT TATGCAAACA ACATACTGGA GAGTGGAATC 
CACGGGTCAC TTATTGCCTT GGATGAGAAC TTTGACTACA CAAGTTTAGC TCTTTTGTTA 
CAAATCCCAA CACAAAATAC TCAGGCACGG CAGATCCTGG AAAGAGAATA CAATAATTTA 
TTGGCTTTGG GAACTGAAAG GCGCCTTGAT GAGAGTGATG ACAAGAACTT CAGACGTGGG 
CCCTCCTGGA GGAGGCAGTT TCCACCACGT GAAGTGCACG GAATCAGCAT GATGCCAGGT 
TCTTCGGAAA CACTGCCAGC AGGATTCAGA TTAACAACAA CATCCGGGCA TTCACGGAAA 
ATGACAACAG ATGTTGCCCC TTCAGGAATA CAGAGATTAG ACAGCTCCAC GGTCCGTACA 
TACTCATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia2 ( XM_012960179.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815633.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), mRNA.

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CloneID	OXa34482
Clone ID Related Accession (Same CDS sequence)	XM_012960179.2
Accession Version	XM_012960179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	liprin-alpha-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAAATATTT TACTTCAGGA ATCAGAGACC 
TTCAGAAAAC AGCTAGAAGA ATGCTTGCAT GATAAGGATC GCCTACAAGA AGAAGTTGAA 
AAATTAAGAT CTGAGGTTGA TCAAGTGAAG ATAAGAACAG GGTCCTTGAT AGAACCTACA 
TTAACGAGGC CTCACCTTGA CTCATCTGCA GAGTTAAGAT ATTCAGTAGG GTCTCTTGTT 
GACAGCCAGT CTGACTACCG CTCAACTAAA GTCATACGAA GACCAAGAAG AGGTAGAATG 
GGCGTTAGGA GAGAAGAGCA ACCGGTGAAA TCACTTGGGG ACCATGAATG GAACCGAACA 
CAGCAGATTG GAGTCCTTAG CAGCCATGCA TTTGAGAGTG ATACTGAAAT GTCCGACATA 
GATGATGATG ACCGTGAAAC AATTTTTAGT TCCATGGACC TCTTATCTCC AGGTGGTCAC 
TCTGATGCTC AAACTTTAGC CATGATGCTT CAAGAACAGC TAGATGCCAT CAATAAAGAA 
ATAAGACTCA TTCAGGAAGA AAAGGAATCT ACTGAACTTC GTGCAGAAGA AATTGAAAAT 
AGAGTAGCCA GTGTTAGCCT AGAGGGGCTC AACCTGGCCA GAATGCACCC AGGTACATCG 
ATCACTGCTT CAGTCACTGC TTCATCATTA GCAAGTTCAT CACCTCCAAG TGGTCATTCT 
ACACCCAAAC TCACACCTCG TAGTCCAGCA AGAGAAATGG ATCGAATGGG GGTCATGACA 
CTACCAAGTG ATTTGAGAAA GCATCGAAGA AAGATTGCAG TTGTAGAAGA AGATGGGCGT 
GAAGATAAAG CCACAATAAA ATGCGAAACA TCCCCTCCTT CAACACCACG TGCTCTCAGG 
ATGACACATA CCTTGCCATC TTCATACCAT AATGATGCCA GAGGAAGTCT GACTGCTTCT 
CTTGAACCTG ATGTGTTTGG TCTTGGGAGC ACAAACAGTA GCCAAGACTC TCTGCACAAA 
GCTCCAAAGA AGAAAGGAAT CAAGTCATCC ATTGGCCGGT TGTTTGGTAA AAAAGAGAAG 
GCTCGATTCG GACAGCTCAG CGCATTTATG GATGCTGATC AAGCGGCACA AGATTCCTTG 
GGCTTAGGAA AACTTGGAAC TCAAGCAGAA AAAGACAGAA GGCTCAAAAA GAAACATGAA 
CTTCTGGAAG AAGCAAGAAG AAAGGGACTT CCATTTGCAC AGTGGGATGG GCCAACCGTA 
GTTGCATGGC TTGAACTTTG GTTGGGAATG CCAGCTTGGT ATGTTGCTGC ATGTCGTGCC 
AACGTAAAGA GTGGTGCCAT AATGTCTGCC TTGTCTGATA CGGAAATACA AAGGGAGATT 
GGGATTAGCA ATCCTCTGCA TCGTTTAAAG CTACGGTTAG CAATCCAAGA GATGGTTTCA 
CTCACCAGCC CATCAGCGCC TCCAACATCC AGAACACCAT CAGGTAATGT CTGGGTAACC 
CATGAGGAAA TGGAGAACCT TACTGCTCCC ACCAAAACGA AAGAGTCAGA AGAAGGCAGC 
TGGGCACAAA CATTGGCATA TGGCGATATG AACCATGAGT GGATTGGGAA TGAATGGCTT 
CCTAGTCTGG GCTTACCGCA GTACAGAAGT TACTTCATGG AATGCCTTGT AGATGCCCGG 
ATGCTAGACC ATTTAACAAA AAAAGATCTT CGAGTGCACT TAAAAATGGT GGATAGCTTT 
CATCGAACAA GTTTGCAGTA TGGCATAATG TGCTTGAAAA AATTAAACTA TGATAGGAAG 
GAGCTGGAAA GGAGAAGAGA AACAAGCCAG CATGAAATTA AAGATGTGTT AGTGTGGAGC 
AATGATCGTG TGATTCGCTG GATCCAAGCA ATAGGCCTTA GAGATTATGC AAACAACATA 
CTGGAGAGTG GAATCCACGG GTCACTTATT GCCTTGGATG AGAACTTTGA CTACACAAGT 
TTAGCTCTTT TGTTACAAAT CCCAACACAA AATACTCAGG CACGGCAGAT CCTGGAAAGA 
GAATACAATA ATTTATTGGC TTTGGGAACT GAAAGGCGCC TTGATGAGAG TGATGACAAG 
AACTTCAGAC GTGGGCCCTC CTGGAGGAGG CAGTTTCCAC CACGTGAAGT GCACGGAATC 
AGCATGATGC CAGGTTCTTC GGAAACACTG CCAGCAGGAT TCAGATTAAC AACAACATCC 
GGGCATTCAC GGAAAATGAC AACAGATGTT GCCCCTTCAG GAATACAGAG ATTAGACAGC 
TCCACGGTCC GTACATACTC ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815633.1
CDS	395..3961
Translation	MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLGLAQQRLQDVIYDRDSLQRQLNSALPQEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELTAANQEIVALREQNAHIQRKMGTGEGPEQEALEGMEQGQKIHDKRLSNGSIDSNDDASQMVELQELLEKQNYEMTQMKERLSSLSARVGEVEQEAESARKELVKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSAQREATSIHDINDKLENELANKEAILRQMEEKNRQLQERLEMAEQKLQQTMRKAETLPEVEAELAQRIAALTKAEERHGNIEERMRHLEAQLEEKNQELQRNILLQESETFRKQLEECLHDKDRLQEEVEKLRSEVDQVKIRTGSLIEPTLTRPHLDSSAELRYSVGSLVDSQSDYRSTKVIRRPRRGRMGVRREEQPVKSLGDHEWNRTQQIGVLSSHAFESDTEMSDIDDDDRETIFSSMDLLSPGGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARMHPGTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIKCETSPPSTPRALRMTHTLPSSYHNDARGSLTASLEPDVFGLGSTNSSQDSLHKAPKKKGIKSSIGRLFGKKEKARFGQLSAFMDADQAAQDSLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELWLGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNVWVTHEEMENLTAPTKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKKLNYDRKELERRRETSQHEIKDVLVWSNDRVIRWIQAIGLRDYANNILESGIHGSLIALDENFDYTSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGPSWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGHSRKMTTDVAPSGIQRLDSSTVRTYSC

Target ORF information:

RefSeq Version	XM_012960179.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (ppfia2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960179.2

ORF Insert Sequence:

ATGATGTGTG AAGTAATGCC GACGATAAAC GAGGACACCC CTATGAGCCA AAGGGGGTCC 
CAAAGCAGTG GCTCGGACTC GGATTCTCAT TTTGAACAGC TGATGGTGAA CATGCTGGAT 
GAAAGGGATC GCCTCCTGGA CACACTGCGT GAAACACAGG AAAGCCTTGG ACTTGCTCAG 
CAAAGACTAC AGGATGTTAT CTATGACAGA GATTCACTCC AGCGACAGCT AAATTCTGCA 
CTGCCACAGG AGTTTGCTGC ATTGACCAAG GAACTGAATG CATGCAGGGA GCAGCTTTTA 
GAAAAAGAAG AAGAGATTTC TGAACTGAAA GCAGAAAGGA ATAACACCAG GCTTTTGTTG 
GAACATTTGG AGTGCCTTGT TTCCAGACAC GAAAGGTCAC TGAGAATGAC AGTGGTAAAG 
CGGCAAGCAC AGTCCCCCTC AGGAGTGTCT AGTGAAGTTG AAGTTCTAAA AGCATTAAAA 
TCATTGTTTG AACATCATAA AGCACTTGAC GAGAAGGTAA GGGAACGGCT GAGGGTTTCT 
TTGGAAAGAG TCTCTGCACT GGAAGAAGAG CTAACTGCTG CAAACCAGGA GATTGTTGCA 
TTAAGAGAAC AAAATGCCCA CATACAAAGA AAGATGGGTA CTGGAGAAGG GCCTGAGCAG 
GAAGCTCTTG AAGGAATGGA GCAAGGACAA AAGATTCATG ATAAGAGACT GTCCAATGGA 
TCGATAGATT CAAATGATGA TGCAAGTCAG ATGGTGGAGC TGCAGGAACT GCTTGAGAAA 
CAAAACTATG AAATGACACA AATGAAAGAG CGATTATCAT CATTGTCAGC CCGTGTTGGG 
GAGGTCGAAC AAGAAGCAGA GTCAGCAAGA AAAGAACTTG TGAAAACTGA AGAGATGAAT 
ACTAAATACC AAAGGGATAT CAGGGAGGCT ATGGCACAAA AGGAGGATAT GGAAGAAAGA 
ATCACTACTT TAGAGAAACG CTATCTCAGT GCACAGAGAG AGGCAACATC CATACATGAC 
ATAAATGACA AGTTGGAAAA TGAACTGGCA AATAAGGAAG CAATTTTACG TCAGATGGAA 
GAGAAAAACA GGCAATTACA AGAGCGGCTG GAAATGGCTG AGCAGAAGTT GCAACAGACT 
ATGAGAAAAG CTGAGACCTT ACCAGAAGTC GAAGCTGAGT TAGCGCAAAG AATTGCTGCA 
CTTACAAAGG CAGAAGAAAG GCATGGGAAT ATTGAAGAAA GGATGAGGCA CCTAGAAGCA 
CAGCTTGAAG AGAAGAACCA AGAACTTCAA AGAAATATTT TACTTCAGGA ATCAGAGACC 
TTCAGAAAAC AGCTAGAAGA ATGCTTGCAT GATAAGGATC GCCTACAAGA AGAAGTTGAA 
AAATTAAGAT CTGAGGTTGA TCAAGTGAAG ATAAGAACAG GGTCCTTGAT AGAACCTACA 
TTAACGAGGC CTCACCTTGA CTCATCTGCA GAGTTAAGAT ATTCAGTAGG GTCTCTTGTT 
GACAGCCAGT CTGACTACCG CTCAACTAAA GTCATACGAA GACCAAGAAG AGGTAGAATG 
GGCGTTAGGA GAGAAGAGCA ACCGGTGAAA TCACTTGGGG ACCATGAATG GAACCGAACA 
CAGCAGATTG GAGTCCTTAG CAGCCATGCA TTTGAGAGTG ATACTGAAAT GTCCGACATA 
GATGATGATG ACCGTGAAAC AATTTTTAGT TCCATGGACC TCTTATCTCC AGGTGGTCAC 
TCTGATGCTC AAACTTTAGC CATGATGCTT CAAGAACAGC TAGATGCCAT CAATAAAGAA 
ATAAGACTCA TTCAGGAAGA AAAGGAATCT ACTGAACTTC GTGCAGAAGA AATTGAAAAT 
AGAGTAGCCA GTGTTAGCCT AGAGGGGCTC AACCTGGCCA GAATGCACCC AGGTACATCG 
ATCACTGCTT CAGTCACTGC TTCATCATTA GCAAGTTCAT CACCTCCAAG TGGTCATTCT 
ACACCCAAAC TCACACCTCG TAGTCCAGCA AGAGAAATGG ATCGAATGGG GGTCATGACA 
CTACCAAGTG ATTTGAGAAA GCATCGAAGA AAGATTGCAG TTGTAGAAGA AGATGGGCGT 
GAAGATAAAG CCACAATAAA ATGCGAAACA TCCCCTCCTT CAACACCACG TGCTCTCAGG 
ATGACACATA CCTTGCCATC TTCATACCAT AATGATGCCA GAGGAAGTCT GACTGCTTCT 
CTTGAACCTG ATGTGTTTGG TCTTGGGAGC ACAAACAGTA GCCAAGACTC TCTGCACAAA 
GCTCCAAAGA AGAAAGGAAT CAAGTCATCC ATTGGCCGGT TGTTTGGTAA AAAAGAGAAG 
GCTCGATTCG GACAGCTCAG CGCATTTATG GATGCTGATC AAGCGGCACA AGATTCCTTG 
GGCTTAGGAA AACTTGGAAC TCAAGCAGAA AAAGACAGAA GGCTCAAAAA GAAACATGAA 
CTTCTGGAAG AAGCAAGAAG AAAGGGACTT CCATTTGCAC AGTGGGATGG GCCAACCGTA 
GTTGCATGGC TTGAACTTTG GTTGGGAATG CCAGCTTGGT ATGTTGCTGC ATGTCGTGCC 
AACGTAAAGA GTGGTGCCAT AATGTCTGCC TTGTCTGATA CGGAAATACA AAGGGAGATT 
GGGATTAGCA ATCCTCTGCA TCGTTTAAAG CTACGGTTAG CAATCCAAGA GATGGTTTCA 
CTCACCAGCC CATCAGCGCC TCCAACATCC AGAACACCAT CAGGTAATGT CTGGGTAACC 
CATGAGGAAA TGGAGAACCT TACTGCTCCC ACCAAAACGA AAGAGTCAGA AGAAGGCAGC 
TGGGCACAAA CATTGGCATA TGGCGATATG AACCATGAGT GGATTGGGAA TGAATGGCTT 
CCTAGTCTGG GCTTACCGCA GTACAGAAGT TACTTCATGG AATGCCTTGT AGATGCCCGG 
ATGCTAGACC ATTTAACAAA AAAAGATCTT CGAGTGCACT TAAAAATGGT GGATAGCTTT 
CATCGAACAA GTTTGCAGTA TGGCATAATG TGCTTGAAAA AATTAAACTA TGATAGGAAG 
GAGCTGGAAA GGAGAAGAGA AACAAGCCAG CATGAAATTA AAGATGTGTT AGTGTGGAGC 
AATGATCGTG TGATTCGCTG GATCCAAGCA ATAGGCCTTA GAGATTATGC AAACAACATA 
CTGGAGAGTG GAATCCACGG GTCACTTATT GCCTTGGATG AGAACTTTGA CTACACAAGT 
TTAGCTCTTT TGTTACAAAT CCCAACACAA AATACTCAGG CACGGCAGAT CCTGGAAAGA 
GAATACAATA ATTTATTGGC TTTGGGAACT GAAAGGCGCC TTGATGAGAG TGATGACAAG 
AACTTCAGAC GTGGGCCCTC CTGGAGGAGG CAGTTTCCAC CACGTGAAGT GCACGGAATC 
AGCATGATGC CAGGTTCTTC GGAAACACTG CCAGCAGGAT TCAGATTAAC AACAACATCC 
GGGCATTCAC GGAAAATGAC AACAGATGTT GCCCCTTCAG GAATACAGAG ATTAGACAGC 
TCCACGGTCC GTACATACTC ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ppfia2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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