ist-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ist-1
Entrez Gene ID	180978
Full Name	Insulin receptor SubsTrate homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001373345.1	NP_001360631.1	Insulin receptor SubsTrate homolog
NM_076800.5	NP_509201.3	Insulin receptor SubsTrate homolog
NM_076800.6	NP_509201.3	Insulin receptor SubsTrate homolog
NM_001330903.2	NP_001317892.1	Insulin receptor SubsTrate homolog
NM_001373346.1	NP_001360716.1	Insulin receptor SubsTrate homolog
NM_001330903.1	NP_001317892.1	Insulin receptor SubsTrate homolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IST-1 appears to be required for signaling through a pathway that may act in parallel to AGE-1/PI3K
Title: Insulin receptor substrate and p55 orthologous adaptor proteins function in the Caenorhabditis elegans daf-2/insulin-like signaling pathway.

The following ist-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ist-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349018	NM_001330903.2 Latest version!	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa349504	NM_076800.6 Latest version!	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa851129	NM_001373345.1 Latest version!	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCa851130	NM_001373346.1 Latest version!	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa349504	NM_076800.5	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCa349018	NM_001330903.1	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ist-1 ( NM_001330903.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317892.1 Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Price & Availability↑

CloneID	OCa349018
Clone ID Related Accession (Same CDS sequence)	NM_001330903.2 , NM_001330903.1
Accession Version	NM_001330903.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Insulin receptor SubsTrate homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001330903.1.

ATGTCTGAAG AAGAAGAATC TACAGATAAT TCTGGGAAAC CCCCTATAGA ACATCCGCAT 
GATAGAGAGG TTAAATCAGA AAAACAATCA TCAAATGTGC TTCCTGGAAT ATTGCGACGA 
GCTGAATCCA CGGATGATGC TCATCAAGTC AGACGATCAT CAATGCGACC ATATGAATCT 
TTCGATGGTT TAAAAAATGA TGGAATACTG AAAAGAAAGA GAAGCTTTTT TGTGAAAACA 
GTGAGCTTCA GCGCAACGGT GTCTGAGCTG ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA 
CCGAAAAATG ACGGAGAGTA CGTTGAGACA ATGTTTGACG CCACGGAAGA GAAGAAACAA 
GAAACGATTG CGCCAAAACA GGAAGAAACT GTAAAGAAAT TATCAATGGC AGCAAGAGAG 
GAGAGAATAC GCGAAAGTAG ACAACAAGGG CAACTGAAAG AAAAAAGGCT GAAAAATCCA 
GATTCAAAAG GTGATACAAG TCACGATAAG CCGACCAGAG AAACATGGAA GCCGTTAGCC 
GTTGAAGACT TACCAAAGGA TGAAGATCCG GATGAATTTG GAATTCCAGA AGTCTACAAA 
TGTGGAAATT GTTTAGTAGG GTTTGCTCCA GTGAAAAAGA AAAAGCTAAT GTTTGTGACA 
CTCACGGAAC GATGTTTAGA ACTTCACGAG TCGGAGAAAT CCTATCGTGC GGGAAAAGCA 
GCAAAGCATA TGGTAGATCT CTCAATGTCT TTTAATGTGC ACAGTGAGCA TTACGATGCA 
AAACTAAAGA AATGCCTATG CTTGATGGGT CCAGACGAAA CAATATGCAT GAGACCGGAA 
GGAGGAATTC TAACCATTGA AGGTTGGCGA CGAGCAATTG TAAAACTAAT TCATGAATCA 
AGAAGAAGAA AAATGGACCG CGTTCCGAGA CCTGAAGATA TTTTTGATGC TGCATATGAT 
GTACGTGTTT GTCTATTTCC AAAAAACTTG GAAAAATACG TGGAAAGCTT GAAAACAGAC 
GGGTTCACAA ACATATGTAC TGTTGCCAAA GAGCTTTTAG GAAAGAAAAG ATTATGCCTT 
TATCCAAATA CACTTGCCAT TGTTGATCTC TGCATTGAAC CAACTGCCTA TGGTCTCCCA 
CCTGCCGGGT TTCCCCCATT CCGTGCATCA AGCATGTTCA TTTTGGAGCG AAATACGGTT 
GCTTACTACG GTTTTCGTGA AAACTATTTC TATGTACGAA TTGGAAAGGG AAGCCCCTAT 
CGAGGGTTTG AGTTATTGTT CCAAGTGGAC ACTAACGAAG TTTGTAAGGA AATTTACTCA 
CGTCTTAGAG CACTCGCTGA TCGTGATCTT GAAAACCGGA AACAAGAATC TATACGGAGA 
CCAGAAAGAC CCCGTGTTTC TACTCTTCAA AGTGTGAAAC CGGGCAAAGA AGAAACCGGA 
CTACGTGAAG CACTTCTTGA ATCATATGGC CATTTAAGAA AAAAGAATTC AACACAATCA 
GAGCGAGGAG AGATAGTTGT TCCGGAGCAA GAACGGAAAA TGTCAGACAA CAGGCCTCGG 
CTCAGAACTC AGGATATAAA AACTGTTACA AAGCCTGAAC TGAAATCAGA AGAACCTATA 
CGCCCGAGAC TTCCACCGCT AAAATTCAAT GCTAACAAAC CGTCTGGAGA ATTTTTATTA 
TCGCTCCAAC GGGAGAAAGA GTTAATGGAA GAGGCAAGAA AAAATGGTTA TGATGGAAGA 
ACACACAATC CTGATGGGAC TCCTCGAGAA ATCAAAAAAG ATTTCCTCGA CACTGTTTGT 
CCACAAGCTG CAGCGCCAGA AGTTGTGATC AAAGAGAAAA TCATAAACAA CGATACTTAT 
ACGCTGATGG GTCCAGCCGA CTGGGGAAAA CTGGAAGATA TTGTCAAAGA TGATTACTCG 
GACAGTGGGG ACAGTTGTTA TTCATCAAGA AGAGGAACCG GTAGTCAACC GACCCGACCG 
GCCGCTTCTC ACCTCGCATG TCAAATGCAA AACCGAACAC AGTCATTTGG AGCTAAACAA 
CAAACATTTC AAAACCGACT TCCGCCTACT GTAAATCTGC CTGATTCTGA AAGAAAAATT 
TCGGCTGCCA ACCAAGGAAC GTCAAACCAA CTCGACCTTC CACAAGAAGA TCCAAGAAAA 
AGAGCATTCT CATTGGGAAG TAAAAACTTT TTCAACCTGA TAGGATTGAA TGACTTTCGT 
CGCCTTGTAA GCAAGCGTCA TCGAACATCA TCTCCAAACC ACACCTCTAC TTCTGGTATC 
TCGTTAAACT CATCTAACGC ATCCCCGTCA GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA 
TATTTAGAAC ATGCAAGAAC TGATAGTTTT GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA 
CACACCCAGC GAACCAGTTC ACCGAAAAGA AGATCGGACG AGGATCTGAT CTCAATTGAC 
TTTTCAAGAT TAGGTAAAAA TTCTGCATCA GATACAAAAC GATTTCCTTT TGGTGGAGGA 
GGACCAGGAG GGTCCTTTGA TTATGATAGA GAAAAACGGG AAAAGGAAGA TAATAATAGA 
CGAGACCGAG AAAAAGCAGC AATGGATTTG AAAAGAAAAG AAGAATGGGA AGCGAGAGAA 
TTAGCGGAAA AGAAGAGGGA GTTGGAACGG AAAATTCAAG CAAAGAAAGA TAGAAAATTG 
GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG GATAAAGACC ACGATCATGA TAAGGAGTTT 
CGTCCAAAAG CGGACAGTGG AATTGCTGAT TGTACGCCCA GTTCAAGTTT TTCTGGGAAG 
AAAGATCACA AAAATGATGG TTCTTCTGAT TCTGCATACA TGGACAAAGA AGGGTTGAAG 
TACTTGGCAG ATATCAAAAA ACGGAAAAAA GAGCAAGGTG CCATAGACAC AACAAATTCC 
TCCTCCTCCT CGTTAAGCAC AATAGTTTCG ATTGAAGATA ACAAAGTGAC CAGAAGAATC 
ACGGAAACCG TTGAAACAAA AGTTGTGACT GCGGATCTAG CGAAAGCACT TGGTGCAATT 
GACCCGAATA GAAGATCTTC AGCATGCATT GAGATAAATC GAAAACCATC GATTTGTACA 
GCAATAATGG AAGAAAGAGA AGGAGAAGCA TCTGAGACCG ATTCTAGGGG AGAACCATCC 
GACAGGAAGA CGACCGCGGC CCCTGTAACT CGGAAACCTG CATCGACTGG CAGCAGCATG 
AGCCCGTTCA GAAGACTTAA ATTTCTCAGC TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317892.1
CDS	4..3285
Translation	MSEEEESTDNSGKPPIEHPHDREVKSEKQSSNVLPGILRRAESTDDAHQVRRSSMRPYESFDGLKNDGILKRKRSFFVKTVSFSATVSELMLPAEPTSGEPKNDGEYVETMFDATEEKKQETIAPKQEETVKKLSMAAREERIRESRQQGQLKEKRLKNPDSKGDTSHDKPTRETWKPLAVEDLPKDEDPDEFGIPEVYKCGNCLVGFAPVKKKKLMFVTLTERCLELHESEKSYRAGKAAKHMVDLSMSFNVHSEHYDAKLKKCLCLMGPDETICMRPEGGILTIEGWRRAIVKLIHESRRRKMDRVPRPEDIFDAAYDVRVCLFPKNLEKYVESLKTDGFTNICTVAKELLGKKRLCLYPNTLAIVDLCIEPTAYGLPPAGFPPFRASSMFILERNTVAYYGFRENYFYVRIGKGSPYRGFELLFQVDTNEVCKEIYSRLRALADRDLENRKQESIRRPERPRVSTLQSVKPGKEETGLREALLESYGHLRKKNSTQSERGEIVVPEQERKMSDNRPRLRTQDIKTVTKPELKSEEPIRPRLPPLKFNANKPSGEFLLSLQREKELMEEARKNGYDGRTHNPDGTPREIKKDFLDTVCPQAAAPEVVIKEKIINNDTYTLMGPADWGKLEDIVKDDYSDSGDSCYSSRRGTGSQPTRPAASHLACQMQNRTQSFGAKQQTFQNRLPPTVNLPDSERKISAANQGTSNQLDLPQEDPRKRAFSLGSKNFFNLIGLNDFRRLVSKRHRTSSPNHTSTSGISLNSSNASPSASSNFLASSEYLEHARTDSFGSARSSPKSLHTQRTSSPKRRSDEDLISIDFSRLGKNSASDTKRFPFGGGGPGGSFDYDREKREKEDNNRRDREKAAMDLKRKEEWEARELAEKKRELERKIQAKKDRKLEKGRGKDREKDKDHDHDKEFRPKADSGIADCTPSSSFSGKKDHKNDGSSDSAYMDKEGLKYLADIKKRKKEQGAIDTTNSSSSSLSTIVSIEDNKVTRRITETVETKVVTADLAKALGAIDPNRRSSACIEINRKPSICTAIMEEREGEASETDSRGEPSDRKTTAAPVTRKPASTGSSMSPFRRLKFLSFRK

Target ORF information:

RefSeq Version	NM_001330903.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330903.2

ORF Insert Sequence:

ATGTCTGAAG AAGAAGAATC TACAGATAAT TCTGGGAAAC CCCCTATAGA ACATCCGCAT 
GATAGAGAGG TTAAATCAGA AAAACAATCA TCAAATGTGC TTCCTGGAAT ATTGCGACGA 
GCTGAATCCA CGGATGATGC TCATCAAGTC AGACGATCAT CAATGCGACC ATATGAATCT 
TTCGATGGTT TAAAAAATGA TGGAATACTG AAAAGAAAGA GAAGCTTTTT TGTGAAAACA 
GTGAGCTTCA GCGCAACGGT GTCTGAGCTG ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA 
CCGAAAAATG ACGGAGAGTA CGTTGAGACA ATGTTTGACG CCACGGAAGA GAAGAAACAA 
GAAACGATTG CGCCAAAACA GGAAGAAACT GTAAAGAAAT TATCAATGGC AGCAAGAGAG 
GAGAGAATAC GCGAAAGTAG ACAACAAGGG CAACTGAAAG AAAAAAGGCT GAAAAATCCA 
GATTCAAAAG GTGATACAAG TCACGATAAG CCGACCAGAG AAACATGGAA GCCGTTAGCC 
GTTGAAGACT TACCAAAGGA TGAAGATCCG GATGAATTTG GAATTCCAGA AGTCTACAAA 
TGTGGAAATT GTTTAGTAGG GTTTGCTCCA GTGAAAAAGA AAAAGCTAAT GTTTGTGACA 
CTCACGGAAC GATGTTTAGA ACTTCACGAG TCGGAGAAAT CCTATCGTGC GGGAAAAGCA 
GCAAAGCATA TGGTAGATCT CTCAATGTCT TTTAATGTGC ACAGTGAGCA TTACGATGCA 
AAACTAAAGA AATGCCTATG CTTGATGGGT CCAGACGAAA CAATATGCAT GAGACCGGAA 
GGAGGAATTC TAACCATTGA AGGTTGGCGA CGAGCAATTG TAAAACTAAT TCATGAATCA 
AGAAGAAGAA AAATGGACCG CGTTCCGAGA CCTGAAGATA TTTTTGATGC TGCATATGAT 
GTACGTGTTT GTCTATTTCC AAAAAACTTG GAAAAATACG TGGAAAGCTT GAAAACAGAC 
GGGTTCACAA ACATATGTAC TGTTGCCAAA GAGCTTTTAG GAAAGAAAAG ATTATGCCTT 
TATCCAAATA CACTTGCCAT TGTTGATCTC TGCATTGAAC CAACTGCCTA TGGTCTCCCA 
CCTGCCGGGT TTCCCCCATT CCGTGCATCA AGCATGTTCA TTTTGGAGCG AAATACGGTT 
GCTTACTACG GTTTTCGTGA AAACTATTTC TATGTACGAA TTGGAAAGGG AAGCCCCTAT 
CGAGGGTTTG AGTTATTGTT CCAAGTGGAC ACTAACGAAG TTTGTAAGGA AATTTACTCA 
CGTCTTAGAG CACTCGCTGA TCGTGATCTT GAAAACCGGA AACAAGAATC TATACGGAGA 
CCAGAAAGAC CCCGTGTTTC TACTCTTCAA AGTGTGAAAC CGGGCAAAGA AGAAACCGGA 
CTACGTGAAG CACTTCTTGA ATCATATGGC CATTTAAGAA AAAAGAATTC AACACAATCA 
GAGCGAGGAG AGATAGTTGT TCCGGAGCAA GAACGGAAAA TGTCAGACAA CAGGCCTCGG 
CTCAGAACTC AGGATATAAA AACTGTTACA AAGCCTGAAC TGAAATCAGA AGAACCTATA 
CGCCCGAGAC TTCCACCGCT AAAATTCAAT GCTAACAAAC CGTCTGGAGA ATTTTTATTA 
TCGCTCCAAC GGGAGAAAGA GTTAATGGAA GAGGCAAGAA AAAATGGTTA TGATGGAAGA 
ACACACAATC CTGATGGGAC TCCTCGAGAA ATCAAAAAAG ATTTCCTCGA CACTGTTTGT 
CCACAAGCTG CAGCGCCAGA AGTTGTGATC AAAGAGAAAA TCATAAACAA CGATACTTAT 
ACGCTGATGG GTCCAGCCGA CTGGGGAAAA CTGGAAGATA TTGTCAAAGA TGATTACTCG 
GACAGTGGGG ACAGTTGTTA TTCATCAAGA AGAGGAACCG GTAGTCAACC GACCCGACCG 
GCCGCTTCTC ACCTCGCATG TCAAATGCAA AACCGAACAC AGTCATTTGG AGCTAAACAA 
CAAACATTTC AAAACCGACT TCCGCCTACT GTAAATCTGC CTGATTCTGA AAGAAAAATT 
TCGGCTGCCA ACCAAGGAAC GTCAAACCAA CTCGACCTTC CACAAGAAGA TCCAAGAAAA 
AGAGCATTCT CATTGGGAAG TAAAAACTTT TTCAACCTGA TAGGATTGAA TGACTTTCGT 
CGCCTTGTAA GCAAGCGTCA TCGAACATCA TCTCCAAACC ACACCTCTAC TTCTGGTATC 
TCGTTAAACT CATCTAACGC ATCCCCGTCA GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA 
TATTTAGAAC ATGCAAGAAC TGATAGTTTT GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA 
CACACCCAGC GAACCAGTTC ACCGAAAAGA AGATCGGACG AGGATCTGAT CTCAATTGAC 
TTTTCAAGAT TAGGTAAAAA TTCTGCATCA GATACAAAAC GATTTCCTTT TGGTGGAGGA 
GGACCAGGAG GGTCCTTTGA TTATGATAGA GAAAAACGGG AAAAGGAAGA TAATAATAGA 
CGAGACCGAG AAAAAGCAGC AATGGATTTG AAAAGAAAAG AAGAATGGGA AGCGAGAGAA 
TTAGCGGAAA AGAAGAGGGA GTTGGAACGG AAAATTCAAG CAAAGAAAGA TAGAAAATTG 
GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG GATAAAGACC ACGATCATGA TAAGGAGTTT 
CGTCCAAAAG CGGACAGTGG AATTGCTGAT TGTACGCCCA GTTCAAGTTT TTCTGGGAAG 
AAAGATCACA AAAATGATGG TTCTTCTGAT TCTGCATACA TGGACAAAGA AGGGTTGAAG 
TACTTGGCAG ATATCAAAAA ACGGAAAAAA GAGCAAGGTG CCATAGACAC AACAAATTCC 
TCCTCCTCCT CGTTAAGCAC AATAGTTTCG ATTGAAGATA ACAAAGTGAC CAGAAGAATC 
ACGGAAACCG TTGAAACAAA AGTTGTGACT GCGGATCTAG CGAAAGCACT TGGTGCAATT 
GACCCGAATA GAAGATCTTC AGCATGCATT GAGATAAATC GAAAACCATC GATTTGTACA 
GCAATAATGG AAGAAAGAGA AGGAGAAGCA TCTGAGACCG ATTCTAGGGG AGAACCATCC 
GACAGGAAGA CGACCGCGGC CCCTGTAACT CGGAAACCTG CATCGACTGG CAGCAGCATG 
AGCCCGTTCA GAAGACTTAA ATTTCTCAGC TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ist-1 ( NM_001330903.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001317892.1 Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), partial mRNA.

Price & Availability↑

CloneID	OCa349018
Clone ID Related Accession (Same CDS sequence)	NM_001330903.2 , NM_001330903.1
Accession Version	NM_001330903.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Insulin receptor SubsTrate homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGTCTGAAG AAGAAGAATC TACAGATAAT TCTGGGAAAC CCCCTATAGA ACATCCGCAT 
GATAGAGAGG TTAAATCAGA AAAACAATCA TCAAATGTGC TTCCTGGAAT ATTGCGACGA 
GCTGAATCCA CGGATGATGC TCATCAAGTC AGACGATCAT CAATGCGACC ATATGAATCT 
TTCGATGGTT TAAAAAATGA TGGAATACTG AAAAGAAAGA GAAGCTTTTT TGTGAAAACA 
GTGAGCTTCA GCGCAACGGT GTCTGAGCTG ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA 
CCGAAAAATG ACGGAGAGTA CGTTGAGACA ATGTTTGACG CCACGGAAGA GAAGAAACAA 
GAAACGATTG CGCCAAAACA GGAAGAAACT GTAAAGAAAT TATCAATGGC AGCAAGAGAG 
GAGAGAATAC GCGAAAGTAG ACAACAAGGG CAACTGAAAG AAAAAAGGCT GAAAAATCCA 
GATTCAAAAG GTGATACAAG TCACGATAAG CCGACCAGAG AAACATGGAA GCCGTTAGCC 
GTTGAAGACT TACCAAAGGA TGAAGATCCG GATGAATTTG GAATTCCAGA AGTCTACAAA 
TGTGGAAATT GTTTAGTAGG GTTTGCTCCA GTGAAAAAGA AAAAGCTAAT GTTTGTGACA 
CTCACGGAAC GATGTTTAGA ACTTCACGAG TCGGAGAAAT CCTATCGTGC GGGAAAAGCA 
GCAAAGCATA TGGTAGATCT CTCAATGTCT TTTAATGTGC ACAGTGAGCA TTACGATGCA 
AAACTAAAGA AATGCCTATG CTTGATGGGT CCAGACGAAA CAATATGCAT GAGACCGGAA 
GGAGGAATTC TAACCATTGA AGGTTGGCGA CGAGCAATTG TAAAACTAAT TCATGAATCA 
AGAAGAAGAA AAATGGACCG CGTTCCGAGA CCTGAAGATA TTTTTGATGC TGCATATGAT 
GTACGTGTTT GTCTATTTCC AAAAAACTTG GAAAAATACG TGGAAAGCTT GAAAACAGAC 
GGGTTCACAA ACATATGTAC TGTTGCCAAA GAGCTTTTAG GAAAGAAAAG ATTATGCCTT 
TATCCAAATA CACTTGCCAT TGTTGATCTC TGCATTGAAC CAACTGCCTA TGGTCTCCCA 
CCTGCCGGGT TTCCCCCATT CCGTGCATCA AGCATGTTCA TTTTGGAGCG AAATACGGTT 
GCTTACTACG GTTTTCGTGA AAACTATTTC TATGTACGAA TTGGAAAGGG AAGCCCCTAT 
CGAGGGTTTG AGTTATTGTT CCAAGTGGAC ACTAACGAAG TTTGTAAGGA AATTTACTCA 
CGTCTTAGAG CACTCGCTGA TCGTGATCTT GAAAACCGGA AACAAGAATC TATACGGAGA 
CCAGAAAGAC CCCGTGTTTC TACTCTTCAA AGTGTGAAAC CGGGCAAAGA AGAAACCGGA 
CTACGTGAAG CACTTCTTGA ATCATATGGC CATTTAAGAA AAAAGAATTC AACACAATCA 
GAGCGAGGAG AGATAGTTGT TCCGGAGCAA GAACGGAAAA TGTCAGACAA CAGGCCTCGG 
CTCAGAACTC AGGATATAAA AACTGTTACA AAGCCTGAAC TGAAATCAGA AGAACCTATA 
CGCCCGAGAC TTCCACCGCT AAAATTCAAT GCTAACAAAC CGTCTGGAGA ATTTTTATTA 
TCGCTCCAAC GGGAGAAAGA GTTAATGGAA GAGGCAAGAA AAAATGGTTA TGATGGAAGA 
ACACACAATC CTGATGGGAC TCCTCGAGAA ATCAAAAAAG ATTTCCTCGA CACTGTTTGT 
CCACAAGCTG CAGCGCCAGA AGTTGTGATC AAAGAGAAAA TCATAAACAA CGATACTTAT 
ACGCTGATGG GTCCAGCCGA CTGGGGAAAA CTGGAAGATA TTGTCAAAGA TGATTACTCG 
GACAGTGGGG ACAGTTGTTA TTCATCAAGA AGAGGAACCG GTAGTCAACC GACCCGACCG 
GCCGCTTCTC ACCTCGCATG TCAAATGCAA AACCGAACAC AGTCATTTGG AGCTAAACAA 
CAAACATTTC AAAACCGACT TCCGCCTACT GTAAATCTGC CTGATTCTGA AAGAAAAATT 
TCGGCTGCCA ACCAAGGAAC GTCAAACCAA CTCGACCTTC CACAAGAAGA TCCAAGAAAA 
AGAGCATTCT CATTGGGAAG TAAAAACTTT TTCAACCTGA TAGGATTGAA TGACTTTCGT 
CGCCTTGTAA GCAAGCGTCA TCGAACATCA TCTCCAAACC ACACCTCTAC TTCTGGTATC 
TCGTTAAACT CATCTAACGC ATCCCCGTCA GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA 
TATTTAGAAC ATGCAAGAAC TGATAGTTTT GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA 
CACACCCAGC GAACCAGTTC ACCGAAAAGA AGATCGGACG AGGATCTGAT CTCAATTGAC 
TTTTCAAGAT TAGGTAAAAA TTCTGCATCA GATACAAAAC GATTTCCTTT TGGTGGAGGA 
GGACCAGGAG GGTCCTTTGA TTATGATAGA GAAAAACGGG AAAAGGAAGA TAATAATAGA 
CGAGACCGAG AAAAAGCAGC AATGGATTTG AAAAGAAAAG AAGAATGGGA AGCGAGAGAA 
TTAGCGGAAA AGAAGAGGGA GTTGGAACGG AAAATTCAAG CAAAGAAAGA TAGAAAATTG 
GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG GATAAAGACC ACGATCATGA TAAGGAGTTT 
CGTCCAAAAG CGGACAGTGG AATTGCTGAT TGTACGCCCA GTTCAAGTTT TTCTGGGAAG 
AAAGATCACA AAAATGATGG TTCTTCTGAT TCTGCATACA TGGACAAAGA AGGGTTGAAG 
TACTTGGCAG ATATCAAAAA ACGGAAAAAA GAGCAAGGTG CCATAGACAC AACAAATTCC 
TCCTCCTCCT CGTTAAGCAC AATAGTTTCG ATTGAAGATA ACAAAGTGAC CAGAAGAATC 
ACGGAAACCG TTGAAACAAA AGTTGTGACT GCGGATCTAG CGAAAGCACT TGGTGCAATT 
GACCCGAATA GAAGATCTTC AGCATGCATT GAGATAAATC GAAAACCATC GATTTGTACA 
GCAATAATGG AAGAAAGAGA AGGAGAAGCA TCTGAGACCG ATTCTAGGGG AGAACCATCC 
GACAGGAAGA CGACCGCGGC CCCTGTAACT CGGAAACCTG CATCGACTGG CAGCAGCATG 
AGCCCGTTCA GAAGACTTAA ATTTCTCAGC TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317892.1
CDS	1..3282
Translation	MSEEEESTDNSGKPPIEHPHDREVKSEKQSSNVLPGILRRAESTDDAHQVRRSSMRPYESFDGLKNDGILKRKRSFFVKTVSFSATVSELMLPAEPTSGEPKNDGEYVETMFDATEEKKQETIAPKQEETVKKLSMAAREERIRESRQQGQLKEKRLKNPDSKGDTSHDKPTRETWKPLAVEDLPKDEDPDEFGIPEVYKCGNCLVGFAPVKKKKLMFVTLTERCLELHESEKSYRAGKAAKHMVDLSMSFNVHSEHYDAKLKKCLCLMGPDETICMRPEGGILTIEGWRRAIVKLIHESRRRKMDRVPRPEDIFDAAYDVRVCLFPKNLEKYVESLKTDGFTNICTVAKELLGKKRLCLYPNTLAIVDLCIEPTAYGLPPAGFPPFRASSMFILERNTVAYYGFRENYFYVRIGKGSPYRGFELLFQVDTNEVCKEIYSRLRALADRDLENRKQESIRRPERPRVSTLQSVKPGKEETGLREALLESYGHLRKKNSTQSERGEIVVPEQERKMSDNRPRLRTQDIKTVTKPELKSEEPIRPRLPPLKFNANKPSGEFLLSLQREKELMEEARKNGYDGRTHNPDGTPREIKKDFLDTVCPQAAAPEVVIKEKIINNDTYTLMGPADWGKLEDIVKDDYSDSGDSCYSSRRGTGSQPTRPAASHLACQMQNRTQSFGAKQQTFQNRLPPTVNLPDSERKISAANQGTSNQLDLPQEDPRKRAFSLGSKNFFNLIGLNDFRRLVSKRHRTSSPNHTSTSGISLNSSNASPSASSNFLASSEYLEHARTDSFGSARSSPKSLHTQRTSSPKRRSDEDLISIDFSRLGKNSASDTKRFPFGGGGPGGSFDYDREKREKEDNNRRDREKAAMDLKRKEEWEARELAEKKRELERKIQAKKDRKLEKGRGKDREKDKDHDHDKEFRPKADSGIADCTPSSSFSGKKDHKNDGSSDSAYMDKEGLKYLADIKKRKKEQGAIDTTNSSSSSLSTIVSIEDNKVTRRITETVETKVVTADLAKALGAIDPNRRSSACIEINRKPSICTAIMEEREGEASETDSRGEPSDRKTTAAPVTRKPASTGSSMSPFRRLKFLSFRK

Target ORF information:

RefSeq Version	NM_001330903.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330903.1

ORF Insert Sequence:

ATGTCTGAAG AAGAAGAATC TACAGATAAT TCTGGGAAAC CCCCTATAGA ACATCCGCAT 
GATAGAGAGG TTAAATCAGA AAAACAATCA TCAAATGTGC TTCCTGGAAT ATTGCGACGA 
GCTGAATCCA CGGATGATGC TCATCAAGTC AGACGATCAT CAATGCGACC ATATGAATCT 
TTCGATGGTT TAAAAAATGA TGGAATACTG AAAAGAAAGA GAAGCTTTTT TGTGAAAACA 
GTGAGCTTCA GCGCAACGGT GTCTGAGCTG ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA 
CCGAAAAATG ACGGAGAGTA CGTTGAGACA ATGTTTGACG CCACGGAAGA GAAGAAACAA 
GAAACGATTG CGCCAAAACA GGAAGAAACT GTAAAGAAAT TATCAATGGC AGCAAGAGAG 
GAGAGAATAC GCGAAAGTAG ACAACAAGGG CAACTGAAAG AAAAAAGGCT GAAAAATCCA 
GATTCAAAAG GTGATACAAG TCACGATAAG CCGACCAGAG AAACATGGAA GCCGTTAGCC 
GTTGAAGACT TACCAAAGGA TGAAGATCCG GATGAATTTG GAATTCCAGA AGTCTACAAA 
TGTGGAAATT GTTTAGTAGG GTTTGCTCCA GTGAAAAAGA AAAAGCTAAT GTTTGTGACA 
CTCACGGAAC GATGTTTAGA ACTTCACGAG TCGGAGAAAT CCTATCGTGC GGGAAAAGCA 
GCAAAGCATA TGGTAGATCT CTCAATGTCT TTTAATGTGC ACAGTGAGCA TTACGATGCA 
AAACTAAAGA AATGCCTATG CTTGATGGGT CCAGACGAAA CAATATGCAT GAGACCGGAA 
GGAGGAATTC TAACCATTGA AGGTTGGCGA CGAGCAATTG TAAAACTAAT TCATGAATCA 
AGAAGAAGAA AAATGGACCG CGTTCCGAGA CCTGAAGATA TTTTTGATGC TGCATATGAT 
GTACGTGTTT GTCTATTTCC AAAAAACTTG GAAAAATACG TGGAAAGCTT GAAAACAGAC 
GGGTTCACAA ACATATGTAC TGTTGCCAAA GAGCTTTTAG GAAAGAAAAG ATTATGCCTT 
TATCCAAATA CACTTGCCAT TGTTGATCTC TGCATTGAAC CAACTGCCTA TGGTCTCCCA 
CCTGCCGGGT TTCCCCCATT CCGTGCATCA AGCATGTTCA TTTTGGAGCG AAATACGGTT 
GCTTACTACG GTTTTCGTGA AAACTATTTC TATGTACGAA TTGGAAAGGG AAGCCCCTAT 
CGAGGGTTTG AGTTATTGTT CCAAGTGGAC ACTAACGAAG TTTGTAAGGA AATTTACTCA 
CGTCTTAGAG CACTCGCTGA TCGTGATCTT GAAAACCGGA AACAAGAATC TATACGGAGA 
CCAGAAAGAC CCCGTGTTTC TACTCTTCAA AGTGTGAAAC CGGGCAAAGA AGAAACCGGA 
CTACGTGAAG CACTTCTTGA ATCATATGGC CATTTAAGAA AAAAGAATTC AACACAATCA 
GAGCGAGGAG AGATAGTTGT TCCGGAGCAA GAACGGAAAA TGTCAGACAA CAGGCCTCGG 
CTCAGAACTC AGGATATAAA AACTGTTACA AAGCCTGAAC TGAAATCAGA AGAACCTATA 
CGCCCGAGAC TTCCACCGCT AAAATTCAAT GCTAACAAAC CGTCTGGAGA ATTTTTATTA 
TCGCTCCAAC GGGAGAAAGA GTTAATGGAA GAGGCAAGAA AAAATGGTTA TGATGGAAGA 
ACACACAATC CTGATGGGAC TCCTCGAGAA ATCAAAAAAG ATTTCCTCGA CACTGTTTGT 
CCACAAGCTG CAGCGCCAGA AGTTGTGATC AAAGAGAAAA TCATAAACAA CGATACTTAT 
ACGCTGATGG GTCCAGCCGA CTGGGGAAAA CTGGAAGATA TTGTCAAAGA TGATTACTCG 
GACAGTGGGG ACAGTTGTTA TTCATCAAGA AGAGGAACCG GTAGTCAACC GACCCGACCG 
GCCGCTTCTC ACCTCGCATG TCAAATGCAA AACCGAACAC AGTCATTTGG AGCTAAACAA 
CAAACATTTC AAAACCGACT TCCGCCTACT GTAAATCTGC CTGATTCTGA AAGAAAAATT 
TCGGCTGCCA ACCAAGGAAC GTCAAACCAA CTCGACCTTC CACAAGAAGA TCCAAGAAAA 
AGAGCATTCT CATTGGGAAG TAAAAACTTT TTCAACCTGA TAGGATTGAA TGACTTTCGT 
CGCCTTGTAA GCAAGCGTCA TCGAACATCA TCTCCAAACC ACACCTCTAC TTCTGGTATC 
TCGTTAAACT CATCTAACGC ATCCCCGTCA GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA 
TATTTAGAAC ATGCAAGAAC TGATAGTTTT GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA 
CACACCCAGC GAACCAGTTC ACCGAAAAGA AGATCGGACG AGGATCTGAT CTCAATTGAC 
TTTTCAAGAT TAGGTAAAAA TTCTGCATCA GATACAAAAC GATTTCCTTT TGGTGGAGGA 
GGACCAGGAG GGTCCTTTGA TTATGATAGA GAAAAACGGG AAAAGGAAGA TAATAATAGA 
CGAGACCGAG AAAAAGCAGC AATGGATTTG AAAAGAAAAG AAGAATGGGA AGCGAGAGAA 
TTAGCGGAAA AGAAGAGGGA GTTGGAACGG AAAATTCAAG CAAAGAAAGA TAGAAAATTG 
GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG GATAAAGACC ACGATCATGA TAAGGAGTTT 
CGTCCAAAAG CGGACAGTGG AATTGCTGAT TGTACGCCCA GTTCAAGTTT TTCTGGGAAG 
AAAGATCACA AAAATGATGG TTCTTCTGAT TCTGCATACA TGGACAAAGA AGGGTTGAAG 
TACTTGGCAG ATATCAAAAA ACGGAAAAAA GAGCAAGGTG CCATAGACAC AACAAATTCC 
TCCTCCTCCT CGTTAAGCAC AATAGTTTCG ATTGAAGATA ACAAAGTGAC CAGAAGAATC 
ACGGAAACCG TTGAAACAAA AGTTGTGACT GCGGATCTAG CGAAAGCACT TGGTGCAATT 
GACCCGAATA GAAGATCTTC AGCATGCATT GAGATAAATC GAAAACCATC GATTTGTACA 
GCAATAATGG AAGAAAGAGA AGGAGAAGCA TCTGAGACCG ATTCTAGGGG AGAACCATCC 
GACAGGAAGA CGACCGCGGC CCCTGTAACT CGGAAACCTG CATCGACTGG CAGCAGCATG 
AGCCCGTTCA GAAGACTTAA ATTTCTCAGC TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ist-1 ( NM_076800.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509201.3 Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), partial mRNA.

Price & Availability↑

CloneID	OCa349504
Clone ID Related Accession (Same CDS sequence)	NM_076800.5 , NM_076800.6
Accession Version	NM_076800.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Insulin receptor SubsTrate homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_076800.4. COMPLETENESS: incomplete on both ends.

ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA CCGAAAAATG ACGGAGAGTA CGTTGAGACA 
ATGTTTGACG CCACGGAAGA GAAGAAACAA GAAACGATTG CGCCAAAACA GGAAGAAACT 
GTAAAGAAAT TATCAATGGC AGCAAGAGAG GAGAGAATAC GCGAAAGTAG ACAACAAGGG 
CAACTGAAAG AAAAAAGGCT GAAAAATCCA GATTCAAAAG GTGATACAAG TCACGATAAG 
CCGACCAGAG AAACATGGAA GCCGTTAGCC GTTGAAGACT TACCAAAGGA TGAAGATCCG 
GATGAATTTG GAATTCCAGA AGTCTACAAA TGTGGAAATT GTTTAGTAGG GTTTGCTCCA 
GTGAAAAAGA AAAAGCTAAT GTTTGTGACA CTCACGGAAC GATGTTTAGA ACTTCACGAG 
TCGGAGAAAT CCTATCGTGC GGGAAAAGCA GCAAAGCATA TGGTAGATCT CTCAATGTCT 
TTTAATGTGC ACAGTGAGCA TTACGATGCA AAACTAAAGA AATGCCTATG CTTGATGGGT 
CCAGACGAAA CAATATGCAT GAGACCGGAA GGAGGAATTC TAACCATTGA AGGTTGGCGA 
CGAGCAATTG TAAAACTAAT TCATGAATCA AGAAGAAGAA AAATGGACCG CGTTCCGAGA 
CCTGAAGATA TTTTTGATGC TGCATATGAT GTACGTGTTT GTCTATTTCC AAAAAACTTG 
GAAAAATACG TGGAAAGCTT GAAAACAGAC GGGTTCACAA ACATATGTAC TGTTGCCAAA 
GAGCTTTTAG GAAAGAAAAG ATTATGCCTT TATCCAAATA CACTTGCCAT TGTTGATCTC 
TGCATTGAAC CAACTGCCTA TGGTCTCCCA CCTGCCGGGT TTCCCCCATT CCGTGCATCA 
AGCATGTTCA TTTTGGAGCG AAATACGGTT GCTTACTACG GTTTTCGTGA AAACTATTTC 
TATGTACGAA TTGGAAAGGG AAGCCCCTAT CGAGGGTTTG AGTTATTGTT CCAAGTGGAC 
ACTAACGAAG TTTGTAAGGA AATTTACTCA CGTCTTAGAG CACTCGCTGA TCGTGATCTT 
GAAAACCGGA AACAAGAATC TATACGGAGA CCAGAAAGAC CCCGTGTTTC TACTCTTCAA 
AGTGTGAAAC CGGGCAAAGA AGAAACCGGA CTACGTGAAG CACTTCTTGA ATCATATGGC 
CATTTAAGAA AAAAGAATTC AACACAATCA GAGCGAGGAG AGATAGTTGT TCCGGAGCAA 
GAACGGAAAA TGTCAGACAA CAGGCCTCGG CTCAGAACTC AGGATATAAA AACTGTTACA 
AAGCCTGAAC TGAAATCAGA AGAACCTATA CGCCCGAGAC TTCCACCGCT AAAATTCAAT 
GCTAACAAAC CGTCTGGAGA ATTTTTATTA TCGCTCCAAC GGGAGAAAGA GTTAATGGAA 
GAGGCAAGAA AAAATGGTTA TGATGGAAGA ACACACAATC CTGATGGGAC TCCTCGAGAA 
ATCAAAAAAG ATTTCCTCGA CACTGTTTGT CCACAAGCTG CAGCGCCAGA AGTTGTGATC 
AAAGAGAAAA TCATAAACAA CGATACTTAT ACGCTGATGG GTCCAGCCGA CTGGGGAAAA 
CTGGAAGATA TTGTCAAAGA TGATTACTCG GACAGTGGGG ACAGTTGTTA TTCATCAAGA 
AGAGGAACCG GTAGTCAACC GACCCGACCG GCCGCTTCTC ACCTCGCATG TCAAATGCAA 
AACCGAACAC AGTCATTTGG AGCTAAACAA CAAACATTTC AAAACCGACT TCCGCCTACT 
GTAAATCTGC CTGATTCTGA AAGAAAAATT TCGGCTGCCA ACCAAGGAAC GTCAAACCAA 
CTCGACCTTC CACAAGAAGA TCCAAGAAAA AGAGCATTCT CATTGGGAAG TAAAAACTTT 
TTCAACCTGA TAGGATTGAA TGACTTTCGT CGCCTTGTAA GCAAGCGTCA TCGAACATCA 
TCTCCAAACC ACACCTCTAC TTCTGGTATC TCGTTAAACT CATCTAACGC ATCCCCGTCA 
GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA TATTTAGAAC ATGCAAGAAC TGATAGTTTT 
GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA CACACCCAGC GAACCAGTTC ACCGAAAAGA 
AGATCGGACG AGGATCTGAT CTCAATTGAC TTTTCAAGAT TAGGTAAAAA TTCTGCATCA 
GATACAAAAC GATTTCCTTT TGGTGGAGGA GGACCAGGAG GGTCCTTTGA TTATGATAGA 
GAAAAACGGG AAAAGGAAGA TAATAATAGA CGAGACCGAG AAAAAGCAGC AATGGATTTG 
AAAAGAAAAG AAGAATGGGA AGCGAGAGAA TTAGCGGAAA AGAAGAGGGA GTTGGAACGG 
AAAATTCAAG CAAAGAAAGA TAGAAAATTG GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG 
GATAAAGACC ACGATCATGA TAAGGAGTTT CGTCCAAAAG CGGACAGTGG AATTGCTGAT 
TGTACGCCCA GTTCAAGTTT TTCTGGGAAG AAAGATCACA AAAATGATGG TTCTTCTGAT 
TCTGCATACA TGGACAAAGA AGGGTTGAAG TACTTGGCAG ATATCAAAAA ACGGAAAAAA 
GAGCAAGGTG CCATAGACAC AACAAATTCC TCCTCCTCCT CGTTAAGCAC AATAGTTTCG 
ATTGAAGATA ACAAAGTGAC CAGAAGAATC ACGGAAACCG TTGAAACAAA AGTTGTGACT 
GCGGATCTAG CGAAAGCACT TGGTGCAATT GACCCGAATA GAAGATCTTC AGCATGCATT 
GAGATAAATC GAAAACCATC GATTTGTACA GCAATAATGG AAGAAAGAGA AGGAGAAGCA 
TCTGAGACCG ATTCTAGGGG AGAACCATCC GACAGGAAGA CGACCGCGGC CCCTGTAACT 
CGGAAACCTG CATCGACTGG CAGCAGCATG AGCCCGTTCA GAAGACTTAA ATTTCTCAGC 
TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509201.3
CDS	1..3012
Translation	MLPAEPTSGEPKNDGEYVETMFDATEEKKQETIAPKQEETVKKLSMAAREERIRESRQQGQLKEKRLKNPDSKGDTSHDKPTRETWKPLAVEDLPKDEDPDEFGIPEVYKCGNCLVGFAPVKKKKLMFVTLTERCLELHESEKSYRAGKAAKHMVDLSMSFNVHSEHYDAKLKKCLCLMGPDETICMRPEGGILTIEGWRRAIVKLIHESRRRKMDRVPRPEDIFDAAYDVRVCLFPKNLEKYVESLKTDGFTNICTVAKELLGKKRLCLYPNTLAIVDLCIEPTAYGLPPAGFPPFRASSMFILERNTVAYYGFRENYFYVRIGKGSPYRGFELLFQVDTNEVCKEIYSRLRALADRDLENRKQESIRRPERPRVSTLQSVKPGKEETGLREALLESYGHLRKKNSTQSERGEIVVPEQERKMSDNRPRLRTQDIKTVTKPELKSEEPIRPRLPPLKFNANKPSGEFLLSLQREKELMEEARKNGYDGRTHNPDGTPREIKKDFLDTVCPQAAAPEVVIKEKIINNDTYTLMGPADWGKLEDIVKDDYSDSGDSCYSSRRGTGSQPTRPAASHLACQMQNRTQSFGAKQQTFQNRLPPTVNLPDSERKISAANQGTSNQLDLPQEDPRKRAFSLGSKNFFNLIGLNDFRRLVSKRHRTSSPNHTSTSGISLNSSNASPSASSNFLASSEYLEHARTDSFGSARSSPKSLHTQRTSSPKRRSDEDLISIDFSRLGKNSASDTKRFPFGGGGPGGSFDYDREKREKEDNNRRDREKAAMDLKRKEEWEARELAEKKRELERKIQAKKDRKLEKGRGKDREKDKDHDHDKEFRPKADSGIADCTPSSSFSGKKDHKNDGSSDSAYMDKEGLKYLADIKKRKKEQGAIDTTNSSSSSLSTIVSIEDNKVTRRITETVETKVVTADLAKALGAIDPNRRSSACIEINRKPSICTAIMEEREGEASETDSRGEPSDRKTTAAPVTRKPASTGSSMSPFRRLKFLSFRK

Target ORF information:

RefSeq Version	NM_076800.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_076800.5

ORF Insert Sequence:

ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA CCGAAAAATG ACGGAGAGTA CGTTGAGACA 
ATGTTTGACG CCACGGAAGA GAAGAAACAA GAAACGATTG CGCCAAAACA GGAAGAAACT 
GTAAAGAAAT TATCAATGGC AGCAAGAGAG GAGAGAATAC GCGAAAGTAG ACAACAAGGG 
CAACTGAAAG AAAAAAGGCT GAAAAATCCA GATTCAAAAG GTGATACAAG TCACGATAAG 
CCGACCAGAG AAACATGGAA GCCGTTAGCC GTTGAAGACT TACCAAAGGA TGAAGATCCG 
GATGAATTTG GAATTCCAGA AGTCTACAAA TGTGGAAATT GTTTAGTAGG GTTTGCTCCA 
GTGAAAAAGA AAAAGCTAAT GTTTGTGACA CTCACGGAAC GATGTTTAGA ACTTCACGAG 
TCGGAGAAAT CCTATCGTGC GGGAAAAGCA GCAAAGCATA TGGTAGATCT CTCAATGTCT 
TTTAATGTGC ACAGTGAGCA TTACGATGCA AAACTAAAGA AATGCCTATG CTTGATGGGT 
CCAGACGAAA CAATATGCAT GAGACCGGAA GGAGGAATTC TAACCATTGA AGGTTGGCGA 
CGAGCAATTG TAAAACTAAT TCATGAATCA AGAAGAAGAA AAATGGACCG CGTTCCGAGA 
CCTGAAGATA TTTTTGATGC TGCATATGAT GTACGTGTTT GTCTATTTCC AAAAAACTTG 
GAAAAATACG TGGAAAGCTT GAAAACAGAC GGGTTCACAA ACATATGTAC TGTTGCCAAA 
GAGCTTTTAG GAAAGAAAAG ATTATGCCTT TATCCAAATA CACTTGCCAT TGTTGATCTC 
TGCATTGAAC CAACTGCCTA TGGTCTCCCA CCTGCCGGGT TTCCCCCATT CCGTGCATCA 
AGCATGTTCA TTTTGGAGCG AAATACGGTT GCTTACTACG GTTTTCGTGA AAACTATTTC 
TATGTACGAA TTGGAAAGGG AAGCCCCTAT CGAGGGTTTG AGTTATTGTT CCAAGTGGAC 
ACTAACGAAG TTTGTAAGGA AATTTACTCA CGTCTTAGAG CACTCGCTGA TCGTGATCTT 
GAAAACCGGA AACAAGAATC TATACGGAGA CCAGAAAGAC CCCGTGTTTC TACTCTTCAA 
AGTGTGAAAC CGGGCAAAGA AGAAACCGGA CTACGTGAAG CACTTCTTGA ATCATATGGC 
CATTTAAGAA AAAAGAATTC AACACAATCA GAGCGAGGAG AGATAGTTGT TCCGGAGCAA 
GAACGGAAAA TGTCAGACAA CAGGCCTCGG CTCAGAACTC AGGATATAAA AACTGTTACA 
AAGCCTGAAC TGAAATCAGA AGAACCTATA CGCCCGAGAC TTCCACCGCT AAAATTCAAT 
GCTAACAAAC CGTCTGGAGA ATTTTTATTA TCGCTCCAAC GGGAGAAAGA GTTAATGGAA 
GAGGCAAGAA AAAATGGTTA TGATGGAAGA ACACACAATC CTGATGGGAC TCCTCGAGAA 
ATCAAAAAAG ATTTCCTCGA CACTGTTTGT CCACAAGCTG CAGCGCCAGA AGTTGTGATC 
AAAGAGAAAA TCATAAACAA CGATACTTAT ACGCTGATGG GTCCAGCCGA CTGGGGAAAA 
CTGGAAGATA TTGTCAAAGA TGATTACTCG GACAGTGGGG ACAGTTGTTA TTCATCAAGA 
AGAGGAACCG GTAGTCAACC GACCCGACCG GCCGCTTCTC ACCTCGCATG TCAAATGCAA 
AACCGAACAC AGTCATTTGG AGCTAAACAA CAAACATTTC AAAACCGACT TCCGCCTACT 
GTAAATCTGC CTGATTCTGA AAGAAAAATT TCGGCTGCCA ACCAAGGAAC GTCAAACCAA 
CTCGACCTTC CACAAGAAGA TCCAAGAAAA AGAGCATTCT CATTGGGAAG TAAAAACTTT 
TTCAACCTGA TAGGATTGAA TGACTTTCGT CGCCTTGTAA GCAAGCGTCA TCGAACATCA 
TCTCCAAACC ACACCTCTAC TTCTGGTATC TCGTTAAACT CATCTAACGC ATCCCCGTCA 
GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA TATTTAGAAC ATGCAAGAAC TGATAGTTTT 
GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA CACACCCAGC GAACCAGTTC ACCGAAAAGA 
AGATCGGACG AGGATCTGAT CTCAATTGAC TTTTCAAGAT TAGGTAAAAA TTCTGCATCA 
GATACAAAAC GATTTCCTTT TGGTGGAGGA GGACCAGGAG GGTCCTTTGA TTATGATAGA 
GAAAAACGGG AAAAGGAAGA TAATAATAGA CGAGACCGAG AAAAAGCAGC AATGGATTTG 
AAAAGAAAAG AAGAATGGGA AGCGAGAGAA TTAGCGGAAA AGAAGAGGGA GTTGGAACGG 
AAAATTCAAG CAAAGAAAGA TAGAAAATTG GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG 
GATAAAGACC ACGATCATGA TAAGGAGTTT CGTCCAAAAG CGGACAGTGG AATTGCTGAT 
TGTACGCCCA GTTCAAGTTT TTCTGGGAAG AAAGATCACA AAAATGATGG TTCTTCTGAT 
TCTGCATACA TGGACAAAGA AGGGTTGAAG TACTTGGCAG ATATCAAAAA ACGGAAAAAA 
GAGCAAGGTG CCATAGACAC AACAAATTCC TCCTCCTCCT CGTTAAGCAC AATAGTTTCG 
ATTGAAGATA ACAAAGTGAC CAGAAGAATC ACGGAAACCG TTGAAACAAA AGTTGTGACT 
GCGGATCTAG CGAAAGCACT TGGTGCAATT GACCCGAATA GAAGATCTTC AGCATGCATT 
GAGATAAATC GAAAACCATC GATTTGTACA GCAATAATGG AAGAAAGAGA AGGAGAAGCA 
TCTGAGACCG ATTCTAGGGG AGAACCATCC GACAGGAAGA CGACCGCGGC CCCTGTAACT 
CGGAAACCTG CATCGACTGG CAGCAGCATG AGCCCGTTCA GAAGACTTAA ATTTCTCAGC 
TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ist-1 ( NM_076800.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509201.3 Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Price & Availability↑

CloneID	OCa349504
Clone ID Related Accession (Same CDS sequence)	NM_076800.5 , NM_076800.6
Accession Version	NM_076800.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Insulin receptor SubsTrate homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_076800.5.

ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA CCGAAAAATG ACGGAGAGTA CGTTGAGACA 
ATGTTTGACG CCACGGAAGA GAAGAAACAA GAAACGATTG CGCCAAAACA GGAAGAAACT 
GTAAAGAAAT TATCAATGGC AGCAAGAGAG GAGAGAATAC GCGAAAGTAG ACAACAAGGG 
CAACTGAAAG AAAAAAGGCT GAAAAATCCA GATTCAAAAG GTGATACAAG TCACGATAAG 
CCGACCAGAG AAACATGGAA GCCGTTAGCC GTTGAAGACT TACCAAAGGA TGAAGATCCG 
GATGAATTTG GAATTCCAGA AGTCTACAAA TGTGGAAATT GTTTAGTAGG GTTTGCTCCA 
GTGAAAAAGA AAAAGCTAAT GTTTGTGACA CTCACGGAAC GATGTTTAGA ACTTCACGAG 
TCGGAGAAAT CCTATCGTGC GGGAAAAGCA GCAAAGCATA TGGTAGATCT CTCAATGTCT 
TTTAATGTGC ACAGTGAGCA TTACGATGCA AAACTAAAGA AATGCCTATG CTTGATGGGT 
CCAGACGAAA CAATATGCAT GAGACCGGAA GGAGGAATTC TAACCATTGA AGGTTGGCGA 
CGAGCAATTG TAAAACTAAT TCATGAATCA AGAAGAAGAA AAATGGACCG CGTTCCGAGA 
CCTGAAGATA TTTTTGATGC TGCATATGAT GTACGTGTTT GTCTATTTCC AAAAAACTTG 
GAAAAATACG TGGAAAGCTT GAAAACAGAC GGGTTCACAA ACATATGTAC TGTTGCCAAA 
GAGCTTTTAG GAAAGAAAAG ATTATGCCTT TATCCAAATA CACTTGCCAT TGTTGATCTC 
TGCATTGAAC CAACTGCCTA TGGTCTCCCA CCTGCCGGGT TTCCCCCATT CCGTGCATCA 
AGCATGTTCA TTTTGGAGCG AAATACGGTT GCTTACTACG GTTTTCGTGA AAACTATTTC 
TATGTACGAA TTGGAAAGGG AAGCCCCTAT CGAGGGTTTG AGTTATTGTT CCAAGTGGAC 
ACTAACGAAG TTTGTAAGGA AATTTACTCA CGTCTTAGAG CACTCGCTGA TCGTGATCTT 
GAAAACCGGA AACAAGAATC TATACGGAGA CCAGAAAGAC CCCGTGTTTC TACTCTTCAA 
AGTGTGAAAC CGGGCAAAGA AGAAACCGGA CTACGTGAAG CACTTCTTGA ATCATATGGC 
CATTTAAGAA AAAAGAATTC AACACAATCA GAGCGAGGAG AGATAGTTGT TCCGGAGCAA 
GAACGGAAAA TGTCAGACAA CAGGCCTCGG CTCAGAACTC AGGATATAAA AACTGTTACA 
AAGCCTGAAC TGAAATCAGA AGAACCTATA CGCCCGAGAC TTCCACCGCT AAAATTCAAT 
GCTAACAAAC CGTCTGGAGA ATTTTTATTA TCGCTCCAAC GGGAGAAAGA GTTAATGGAA 
GAGGCAAGAA AAAATGGTTA TGATGGAAGA ACACACAATC CTGATGGGAC TCCTCGAGAA 
ATCAAAAAAG ATTTCCTCGA CACTGTTTGT CCACAAGCTG CAGCGCCAGA AGTTGTGATC 
AAAGAGAAAA TCATAAACAA CGATACTTAT ACGCTGATGG GTCCAGCCGA CTGGGGAAAA 
CTGGAAGATA TTGTCAAAGA TGATTACTCG GACAGTGGGG ACAGTTGTTA TTCATCAAGA 
AGAGGAACCG GTAGTCAACC GACCCGACCG GCCGCTTCTC ACCTCGCATG TCAAATGCAA 
AACCGAACAC AGTCATTTGG AGCTAAACAA CAAACATTTC AAAACCGACT TCCGCCTACT 
GTAAATCTGC CTGATTCTGA AAGAAAAATT TCGGCTGCCA ACCAAGGAAC GTCAAACCAA 
CTCGACCTTC CACAAGAAGA TCCAAGAAAA AGAGCATTCT CATTGGGAAG TAAAAACTTT 
TTCAACCTGA TAGGATTGAA TGACTTTCGT CGCCTTGTAA GCAAGCGTCA TCGAACATCA 
TCTCCAAACC ACACCTCTAC TTCTGGTATC TCGTTAAACT CATCTAACGC ATCCCCGTCA 
GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA TATTTAGAAC ATGCAAGAAC TGATAGTTTT 
GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA CACACCCAGC GAACCAGTTC ACCGAAAAGA 
AGATCGGACG AGGATCTGAT CTCAATTGAC TTTTCAAGAT TAGGTAAAAA TTCTGCATCA 
GATACAAAAC GATTTCCTTT TGGTGGAGGA GGACCAGGAG GGTCCTTTGA TTATGATAGA 
GAAAAACGGG AAAAGGAAGA TAATAATAGA CGAGACCGAG AAAAAGCAGC AATGGATTTG 
AAAAGAAAAG AAGAATGGGA AGCGAGAGAA TTAGCGGAAA AGAAGAGGGA GTTGGAACGG 
AAAATTCAAG CAAAGAAAGA TAGAAAATTG GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG 
GATAAAGACC ACGATCATGA TAAGGAGTTT CGTCCAAAAG CGGACAGTGG AATTGCTGAT 
TGTACGCCCA GTTCAAGTTT TTCTGGGAAG AAAGATCACA AAAATGATGG TTCTTCTGAT 
TCTGCATACA TGGACAAAGA AGGGTTGAAG TACTTGGCAG ATATCAAAAA ACGGAAAAAA 
GAGCAAGGTG CCATAGACAC AACAAATTCC TCCTCCTCCT CGTTAAGCAC AATAGTTTCG 
ATTGAAGATA ACAAAGTGAC CAGAAGAATC ACGGAAACCG TTGAAACAAA AGTTGTGACT 
GCGGATCTAG CGAAAGCACT TGGTGCAATT GACCCGAATA GAAGATCTTC AGCATGCATT 
GAGATAAATC GAAAACCATC GATTTGTACA GCAATAATGG AAGAAAGAGA AGGAGAAGCA 
TCTGAGACCG ATTCTAGGGG AGAACCATCC GACAGGAAGA CGACCGCGGC CCCTGTAACT 
CGGAAACCTG CATCGACTGG CAGCAGCATG AGCCCGTTCA GAAGACTTAA ATTTCTCAGC 
TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509201.3
CDS	56..3067
Translation	MLPAEPTSGEPKNDGEYVETMFDATEEKKQETIAPKQEETVKKLSMAAREERIRESRQQGQLKEKRLKNPDSKGDTSHDKPTRETWKPLAVEDLPKDEDPDEFGIPEVYKCGNCLVGFAPVKKKKLMFVTLTERCLELHESEKSYRAGKAAKHMVDLSMSFNVHSEHYDAKLKKCLCLMGPDETICMRPEGGILTIEGWRRAIVKLIHESRRRKMDRVPRPEDIFDAAYDVRVCLFPKNLEKYVESLKTDGFTNICTVAKELLGKKRLCLYPNTLAIVDLCIEPTAYGLPPAGFPPFRASSMFILERNTVAYYGFRENYFYVRIGKGSPYRGFELLFQVDTNEVCKEIYSRLRALADRDLENRKQESIRRPERPRVSTLQSVKPGKEETGLREALLESYGHLRKKNSTQSERGEIVVPEQERKMSDNRPRLRTQDIKTVTKPELKSEEPIRPRLPPLKFNANKPSGEFLLSLQREKELMEEARKNGYDGRTHNPDGTPREIKKDFLDTVCPQAAAPEVVIKEKIINNDTYTLMGPADWGKLEDIVKDDYSDSGDSCYSSRRGTGSQPTRPAASHLACQMQNRTQSFGAKQQTFQNRLPPTVNLPDSERKISAANQGTSNQLDLPQEDPRKRAFSLGSKNFFNLIGLNDFRRLVSKRHRTSSPNHTSTSGISLNSSNASPSASSNFLASSEYLEHARTDSFGSARSSPKSLHTQRTSSPKRRSDEDLISIDFSRLGKNSASDTKRFPFGGGGPGGSFDYDREKREKEDNNRRDREKAAMDLKRKEEWEARELAEKKRELERKIQAKKDRKLEKGRGKDREKDKDHDHDKEFRPKADSGIADCTPSSSFSGKKDHKNDGSSDSAYMDKEGLKYLADIKKRKKEQGAIDTTNSSSSSLSTIVSIEDNKVTRRITETVETKVVTADLAKALGAIDPNRRSSACIEINRKPSICTAIMEEREGEASETDSRGEPSDRKTTAAPVTRKPASTGSSMSPFRRLKFLSFRK

Target ORF information:

RefSeq Version	NM_076800.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_076800.6

ORF Insert Sequence:

ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA CCGAAAAATG ACGGAGAGTA CGTTGAGACA 
ATGTTTGACG CCACGGAAGA GAAGAAACAA GAAACGATTG CGCCAAAACA GGAAGAAACT 
GTAAAGAAAT TATCAATGGC AGCAAGAGAG GAGAGAATAC GCGAAAGTAG ACAACAAGGG 
CAACTGAAAG AAAAAAGGCT GAAAAATCCA GATTCAAAAG GTGATACAAG TCACGATAAG 
CCGACCAGAG AAACATGGAA GCCGTTAGCC GTTGAAGACT TACCAAAGGA TGAAGATCCG 
GATGAATTTG GAATTCCAGA AGTCTACAAA TGTGGAAATT GTTTAGTAGG GTTTGCTCCA 
GTGAAAAAGA AAAAGCTAAT GTTTGTGACA CTCACGGAAC GATGTTTAGA ACTTCACGAG 
TCGGAGAAAT CCTATCGTGC GGGAAAAGCA GCAAAGCATA TGGTAGATCT CTCAATGTCT 
TTTAATGTGC ACAGTGAGCA TTACGATGCA AAACTAAAGA AATGCCTATG CTTGATGGGT 
CCAGACGAAA CAATATGCAT GAGACCGGAA GGAGGAATTC TAACCATTGA AGGTTGGCGA 
CGAGCAATTG TAAAACTAAT TCATGAATCA AGAAGAAGAA AAATGGACCG CGTTCCGAGA 
CCTGAAGATA TTTTTGATGC TGCATATGAT GTACGTGTTT GTCTATTTCC AAAAAACTTG 
GAAAAATACG TGGAAAGCTT GAAAACAGAC GGGTTCACAA ACATATGTAC TGTTGCCAAA 
GAGCTTTTAG GAAAGAAAAG ATTATGCCTT TATCCAAATA CACTTGCCAT TGTTGATCTC 
TGCATTGAAC CAACTGCCTA TGGTCTCCCA CCTGCCGGGT TTCCCCCATT CCGTGCATCA 
AGCATGTTCA TTTTGGAGCG AAATACGGTT GCTTACTACG GTTTTCGTGA AAACTATTTC 
TATGTACGAA TTGGAAAGGG AAGCCCCTAT CGAGGGTTTG AGTTATTGTT CCAAGTGGAC 
ACTAACGAAG TTTGTAAGGA AATTTACTCA CGTCTTAGAG CACTCGCTGA TCGTGATCTT 
GAAAACCGGA AACAAGAATC TATACGGAGA CCAGAAAGAC CCCGTGTTTC TACTCTTCAA 
AGTGTGAAAC CGGGCAAAGA AGAAACCGGA CTACGTGAAG CACTTCTTGA ATCATATGGC 
CATTTAAGAA AAAAGAATTC AACACAATCA GAGCGAGGAG AGATAGTTGT TCCGGAGCAA 
GAACGGAAAA TGTCAGACAA CAGGCCTCGG CTCAGAACTC AGGATATAAA AACTGTTACA 
AAGCCTGAAC TGAAATCAGA AGAACCTATA CGCCCGAGAC TTCCACCGCT AAAATTCAAT 
GCTAACAAAC CGTCTGGAGA ATTTTTATTA TCGCTCCAAC GGGAGAAAGA GTTAATGGAA 
GAGGCAAGAA AAAATGGTTA TGATGGAAGA ACACACAATC CTGATGGGAC TCCTCGAGAA 
ATCAAAAAAG ATTTCCTCGA CACTGTTTGT CCACAAGCTG CAGCGCCAGA AGTTGTGATC 
AAAGAGAAAA TCATAAACAA CGATACTTAT ACGCTGATGG GTCCAGCCGA CTGGGGAAAA 
CTGGAAGATA TTGTCAAAGA TGATTACTCG GACAGTGGGG ACAGTTGTTA TTCATCAAGA 
AGAGGAACCG GTAGTCAACC GACCCGACCG GCCGCTTCTC ACCTCGCATG TCAAATGCAA 
AACCGAACAC AGTCATTTGG AGCTAAACAA CAAACATTTC AAAACCGACT TCCGCCTACT 
GTAAATCTGC CTGATTCTGA AAGAAAAATT TCGGCTGCCA ACCAAGGAAC GTCAAACCAA 
CTCGACCTTC CACAAGAAGA TCCAAGAAAA AGAGCATTCT CATTGGGAAG TAAAAACTTT 
TTCAACCTGA TAGGATTGAA TGACTTTCGT CGCCTTGTAA GCAAGCGTCA TCGAACATCA 
TCTCCAAACC ACACCTCTAC TTCTGGTATC TCGTTAAACT CATCTAACGC ATCCCCGTCA 
GCAAGTAGTA ATTTTTTGGC TTCTTCTGAA TATTTAGAAC ATGCAAGAAC TGATAGTTTT 
GGGTCCGCAA GAAGTAGTCC AAAAAGTCTA CACACCCAGC GAACCAGTTC ACCGAAAAGA 
AGATCGGACG AGGATCTGAT CTCAATTGAC TTTTCAAGAT TAGGTAAAAA TTCTGCATCA 
GATACAAAAC GATTTCCTTT TGGTGGAGGA GGACCAGGAG GGTCCTTTGA TTATGATAGA 
GAAAAACGGG AAAAGGAAGA TAATAATAGA CGAGACCGAG AAAAAGCAGC AATGGATTTG 
AAAAGAAAAG AAGAATGGGA AGCGAGAGAA TTAGCGGAAA AGAAGAGGGA GTTGGAACGG 
AAAATTCAAG CAAAGAAAGA TAGAAAATTG GAGAAGGGAA GAGGGAAAGA TAGAGAAAAG 
GATAAAGACC ACGATCATGA TAAGGAGTTT CGTCCAAAAG CGGACAGTGG AATTGCTGAT 
TGTACGCCCA GTTCAAGTTT TTCTGGGAAG AAAGATCACA AAAATGATGG TTCTTCTGAT 
TCTGCATACA TGGACAAAGA AGGGTTGAAG TACTTGGCAG ATATCAAAAA ACGGAAAAAA 
GAGCAAGGTG CCATAGACAC AACAAATTCC TCCTCCTCCT CGTTAAGCAC AATAGTTTCG 
ATTGAAGATA ACAAAGTGAC CAGAAGAATC ACGGAAACCG TTGAAACAAA AGTTGTGACT 
GCGGATCTAG CGAAAGCACT TGGTGCAATT GACCCGAATA GAAGATCTTC AGCATGCATT 
GAGATAAATC GAAAACCATC GATTTGTACA GCAATAATGG AAGAAAGAGA AGGAGAAGCA 
TCTGAGACCG ATTCTAGGGG AGAACCATCC GACAGGAAGA CGACCGCGGC CCCTGTAACT 
CGGAAACCTG CATCGACTGG CAGCAGCATG AGCCCGTTCA GAAGACTTAA ATTTCTCAGC 
TTCAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ist-1 ( NM_001373345.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360631.1 Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Price & Availability↑

CloneID	OCa851129
Clone ID Related Accession (Same CDS sequence)	NM_001373345.1
Accession Version	NM_001373345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Insulin receptor SubsTrate homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284).

ATGTCTGAAG AAGAAGAATC TACAGATAAT TCTGGGAAAC CCCCTATAGA ACATCCGCAT 
GATAGAGAGG TTAAATCAGA AAAACAATCA TCAAATGTGC TTCCTGGAAT ATTGCGACGA 
GCTGAATCCA CGGATGATGC TCATCAAGTC AGACGATCAT CAATGCGACC ATATGAATCT 
TTCGATGGTT TAAAAAATGA TGGAATACTG AAAAGAAAGA GAAGCTTTTT TGTGAAAACA 
GTGAGCTTCA GCGCAACGGT GTCTGAGCTG ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA 
CCGAAAAATG ACGGAGAGTA CGTTGAGACA ATGTTTGACG CCACGGAAGA GAAGAAACAA 
GAAACGATTG CGCCAAAACA GGAAGAAACT GTAAAGAAAT TATCAATGGC AGCAAGAGAG 
GAGAGAATAC GCGAAAGTAG ACAACAAGGG CAACTGAAAG AAAAAAGGCT GAAAAATCCA 
GATTCAAAAG GTGATACAAG TCACGATAAG CCGACCAGAG AAACATGGAA GCCGTTAGCC 
GTTGAAGACT TACCAAAGGA TGAAGATCCG GATGAATTTG GAATTCCAGA AGTCTACAAA 
TGTGGAAATT GTTTAGTAGG GTTTGCTCCA GTGAAAAAGA AAAAGCTAAT GTTTGTGACA 
CTCACGGAAC GATGTTTAGA ACTTCACGAG TCGGAGAAAT CCTATCGTGC GGGAAAAGCA 
GCAAAGCATA TGGTAGATCT CTCAATGTCT TTTAATGTGC ACAGTGAGCA TTACGATGCA 
AAACTAAAGA AATGCCTATG CTTGATGGGT CCAGACGAAA CAATATGCAT GAGACCGGAA 
GGAGGAATTC TAACCATTGA AGGTTGGCGA CGAGCAATTG TAAAACTAAT TCATGAATCA 
AGAAGAAGAA AAATGGACCG CGTTCCGAGA CCTGAAGATA TTTTTGATGC TGCATATGAT 
GTACGTGTTT GTCTATTTCC AAAAAACTTG GAAAAATACG TGGAAAGCTT GAAAACAGAC 
GGGTTCACAA ACATATGTAC TGTTGCCAAA GAGCTTTTAG GAAAGAAAAG ATTATGCCTT 
TATCCAAATA CACTTGCCAT TGTTGATCTC TGCATTGAAC CAACTGCCTA TGGTCTCCCA 
CCTGCCGGGT TTCCCCCATT CCGTGCATCA AGCATGTTCA TTTTGGAGCG AAATACGGTT 
GCTTACTACG GTTTTCGTGA AAACTATTTC TATGTACGAA TTGGAAAGGG AAGCCCCTAT 
CGAGGGTTTG AGTTATTGTT CCAAGTGGAC ACTAACGAAG TTTGTAAGGA AATTTACTCA 
CGTCTTAGAG CACTCGCTGA TCGTGATCTT GAAAACCGGA AACAAGAATC TATACGGAGA 
CCAGAAAGTG ACATGCATGG AATGGAATCC CTTTCTGTTC CCTCCCCGCT TTTGCATCGT 
ACCAAACTGT CCCTCGACTC GCCTGTTATA ACTCCCCGCG ACCGTCGCCT TATGTTGGGC 
CGTGAATGCC TATCGTTTGC CTCGCTTGAA AAAGATGATT CTGCGCCATC GTCTCCATTC 
GCCAACTACA ATCGTCCTCG TGGATCATTG GGGAATTTCC AGCTTGATCA CCTGTCTAGA 
TTTGACCAAC CTCGCGGATC AATTCATTCA CTGGGAAACA TTCCGATTGA TAGACCCCGT 
GTTTCTACTC TTCAAAGTGT GAAACCGGGC AAAGAAGAAA CCGGACTACG TGAAGCACTT 
CTTGAATCAT ATGGCCATTT AAGAAAAAAG AATTCAACAC AATCAGAGCG AGGAGAGATA 
GTTGTTCCGG AGCAAGAACG GAAAATGTCA GACAACAGGC CTCGGCTCAG AACTCAGGAT 
ATAAAAACTG TTACAAAGCC TGAACTGAAA TCAGAAGAAC CTATACGCCC GAGACTTCCA 
CCGCTAAAAT TCAATGCTAA CAAACCGTCT GGAGAATTTT TATTATCGCT CCAACGGGAG 
AAAGAGTTAA TGGAAGAGGC AAGAAAAAAT GGTTATGATG GAAGAACACA CAATCCTGAT 
GGGACTCCTC GAGAAATCAA AAAAGATTTC CTCGACACTG TTTGTCCACA AGCTGCAGCG 
CCAGAAGTTG TGATCAAAGA GAAAATCATA AACAACGATA CTTATACGCT GATGGGTCCA 
GCCGACTGGG GAAAACTGGA AGATATTGTC AAAGATGATT ACTCGGACAG TGGGGACAGT 
TGTTATTCAT CAAGAAGAGG AACCGGTAGT CAACCGACCC GACCGGCCGC TTCTCACCTC 
GCATGTCAAA TGCAAAACCG AACACAGTCA TTTGGAGCTA AACAACAAAC ATTTCAAAAC 
CGACTTCCGC CTACTGTAAA TCTGCCTGAT TCTGAAAGAA AAATTTCGGC TGCCAACCAA 
GGAACGTCAA ACCAACTCGA CCTTCCACAA GAAGATCCAA GAAAAAGAGC ATTCTCATTG 
GGAAGTAAAA ACTTTTTCAA CCTGATAGGA TTGAATGACT TTCGTCGCCT TGTAAGCAAG 
CGTCATCGAA CATCATCTCC AAACCACACC TCTACTTCTG GTATCTCGTT AAACTCATCT 
AACGCATCCC CGTCAGCAAG TAGTAATTTT TTGGCTTCTT CTGAATATTT AGAACATGCA 
AGAACTGATA GTTTTGGGTC CGCAAGAAGT AGTCCAAAAA GTCTACACAC CCAGCGAACC 
AGTTCACCGA AAAGAAGATC GGACGAGGAT CTGATCTCAA TTGACTTTTC AAGATTAGGT 
AAAAATTCTG CATCAGATAC AAAACGATTT CCTTTTGGTG GAGGAGGACC AGGAGGGTCC 
TTTGATTATG ATAGAGAAAA ACGGGAAAAG GAAGATAATA ATAGACGAGA CCGAGAAAAA 
GCAGCAATGG ATTTGAAAAG AAAAGAAGAA TGGGAAGCGA GAGAATTAGC GGAAAAGAAG 
AGGGAGTTGG AACGGAAAAT TCAAGCAAAG AAAGATAGAA AATTGGAGAA GGGAAGAGGG 
AAAGATAGAG AAAAGGATAA AGACCACGAT CATGATAAGG AGTTTCGTCC AAAAGCGGAC 
AGTGGAATTG CTGATTGTAC GCCCAGTTCA AGTTTTTCTG GGAAGAAAGA TCACAAAAAT 
GATGGTTCTT CTGATTCTGC ATACATGGAC AAAGAAGGGT TGAAGTACTT GGCAGATATC 
AAAAAACGGA AAAAAGAGCA AGGTGCCATA GACACAACAA ATTCCTCCTC CTCCTCGTTA 
AGCACAATAG TTTCGATTGA AGATAACAAA GTGACCAGAA GAATCACGGA AACCGTTGAA 
ACAAAAGTTG TGACTGCGGA TCTAGCGAAA GCACTTGGTG CAATTGACCC GAATAGAAGA 
TCTTCAGCAT GCATTGAGAT AAATCGAAAA CCATCGATTT GTACAGCAAT AATGGAAGAA 
AGAGAAGGAG AAGCATCTGA GACCGATTCT AGGGGAGAAC CATCCGACAG GAAGACGACC 
GCGGCCCCTG TAACTCGGAA ACCTGCATCG ACTGGCAGCA GCATGAGCCC GTTCAGAAGA 
CTTAAATTTC TCAGCTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360631.1
CDS	4..3570
Translation	MSEEEESTDNSGKPPIEHPHDREVKSEKQSSNVLPGILRRAESTDDAHQVRRSSMRPYESFDGLKNDGILKRKRSFFVKTVSFSATVSELMLPAEPTSGEPKNDGEYVETMFDATEEKKQETIAPKQEETVKKLSMAAREERIRESRQQGQLKEKRLKNPDSKGDTSHDKPTRETWKPLAVEDLPKDEDPDEFGIPEVYKCGNCLVGFAPVKKKKLMFVTLTERCLELHESEKSYRAGKAAKHMVDLSMSFNVHSEHYDAKLKKCLCLMGPDETICMRPEGGILTIEGWRRAIVKLIHESRRRKMDRVPRPEDIFDAAYDVRVCLFPKNLEKYVESLKTDGFTNICTVAKELLGKKRLCLYPNTLAIVDLCIEPTAYGLPPAGFPPFRASSMFILERNTVAYYGFRENYFYVRIGKGSPYRGFELLFQVDTNEVCKEIYSRLRALADRDLENRKQESIRRPESDMHGMESLSVPSPLLHRTKLSLDSPVITPRDRRLMLGRECLSFASLEKDDSAPSSPFANYNRPRGSLGNFQLDHLSRFDQPRGSIHSLGNIPIDRPRVSTLQSVKPGKEETGLREALLESYGHLRKKNSTQSERGEIVVPEQERKMSDNRPRLRTQDIKTVTKPELKSEEPIRPRLPPLKFNANKPSGEFLLSLQREKELMEEARKNGYDGRTHNPDGTPREIKKDFLDTVCPQAAAPEVVIKEKIINNDTYTLMGPADWGKLEDIVKDDYSDSGDSCYSSRRGTGSQPTRPAASHLACQMQNRTQSFGAKQQTFQNRLPPTVNLPDSERKISAANQGTSNQLDLPQEDPRKRAFSLGSKNFFNLIGLNDFRRLVSKRHRTSSPNHTSTSGISLNSSNASPSASSNFLASSEYLEHARTDSFGSARSSPKSLHTQRTSSPKRRSDEDLISIDFSRLGKNSASDTKRFPFGGGGPGGSFDYDREKREKEDNNRRDREKAAMDLKRKEEWEARELAEKKRELERKIQAKKDRKLEKGRGKDREKDKDHDHDKEFRPKADSGIADCTPSSSFSGKKDHKNDGSSDSAYMDKEGLKYLADIKKRKKEQGAIDTTNSSSSSLSTIVSIEDNKVTRRITETVETKVVTADLAKALGAIDPNRRSSACIEINRKPSICTAIMEEREGEASETDSRGEPSDRKTTAAPVTRKPASTGSSMSPFRRLKFLSFRK

Target ORF information:

RefSeq Version	NM_001373345.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373345.1

ORF Insert Sequence:

ATGTCTGAAG AAGAAGAATC TACAGATAAT TCTGGGAAAC CCCCTATAGA ACATCCGCAT 
GATAGAGAGG TTAAATCAGA AAAACAATCA TCAAATGTGC TTCCTGGAAT ATTGCGACGA 
GCTGAATCCA CGGATGATGC TCATCAAGTC AGACGATCAT CAATGCGACC ATATGAATCT 
TTCGATGGTT TAAAAAATGA TGGAATACTG AAAAGAAAGA GAAGCTTTTT TGTGAAAACA 
GTGAGCTTCA GCGCAACGGT GTCTGAGCTG ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA 
CCGAAAAATG ACGGAGAGTA CGTTGAGACA ATGTTTGACG CCACGGAAGA GAAGAAACAA 
GAAACGATTG CGCCAAAACA GGAAGAAACT GTAAAGAAAT TATCAATGGC AGCAAGAGAG 
GAGAGAATAC GCGAAAGTAG ACAACAAGGG CAACTGAAAG AAAAAAGGCT GAAAAATCCA 
GATTCAAAAG GTGATACAAG TCACGATAAG CCGACCAGAG AAACATGGAA GCCGTTAGCC 
GTTGAAGACT TACCAAAGGA TGAAGATCCG GATGAATTTG GAATTCCAGA AGTCTACAAA 
TGTGGAAATT GTTTAGTAGG GTTTGCTCCA GTGAAAAAGA AAAAGCTAAT GTTTGTGACA 
CTCACGGAAC GATGTTTAGA ACTTCACGAG TCGGAGAAAT CCTATCGTGC GGGAAAAGCA 
GCAAAGCATA TGGTAGATCT CTCAATGTCT TTTAATGTGC ACAGTGAGCA TTACGATGCA 
AAACTAAAGA AATGCCTATG CTTGATGGGT CCAGACGAAA CAATATGCAT GAGACCGGAA 
GGAGGAATTC TAACCATTGA AGGTTGGCGA CGAGCAATTG TAAAACTAAT TCATGAATCA 
AGAAGAAGAA AAATGGACCG CGTTCCGAGA CCTGAAGATA TTTTTGATGC TGCATATGAT 
GTACGTGTTT GTCTATTTCC AAAAAACTTG GAAAAATACG TGGAAAGCTT GAAAACAGAC 
GGGTTCACAA ACATATGTAC TGTTGCCAAA GAGCTTTTAG GAAAGAAAAG ATTATGCCTT 
TATCCAAATA CACTTGCCAT TGTTGATCTC TGCATTGAAC CAACTGCCTA TGGTCTCCCA 
CCTGCCGGGT TTCCCCCATT CCGTGCATCA AGCATGTTCA TTTTGGAGCG AAATACGGTT 
GCTTACTACG GTTTTCGTGA AAACTATTTC TATGTACGAA TTGGAAAGGG AAGCCCCTAT 
CGAGGGTTTG AGTTATTGTT CCAAGTGGAC ACTAACGAAG TTTGTAAGGA AATTTACTCA 
CGTCTTAGAG CACTCGCTGA TCGTGATCTT GAAAACCGGA AACAAGAATC TATACGGAGA 
CCAGAAAGTG ACATGCATGG AATGGAATCC CTTTCTGTTC CCTCCCCGCT TTTGCATCGT 
ACCAAACTGT CCCTCGACTC GCCTGTTATA ACTCCCCGCG ACCGTCGCCT TATGTTGGGC 
CGTGAATGCC TATCGTTTGC CTCGCTTGAA AAAGATGATT CTGCGCCATC GTCTCCATTC 
GCCAACTACA ATCGTCCTCG TGGATCATTG GGGAATTTCC AGCTTGATCA CCTGTCTAGA 
TTTGACCAAC CTCGCGGATC AATTCATTCA CTGGGAAACA TTCCGATTGA TAGACCCCGT 
GTTTCTACTC TTCAAAGTGT GAAACCGGGC AAAGAAGAAA CCGGACTACG TGAAGCACTT 
CTTGAATCAT ATGGCCATTT AAGAAAAAAG AATTCAACAC AATCAGAGCG AGGAGAGATA 
GTTGTTCCGG AGCAAGAACG GAAAATGTCA GACAACAGGC CTCGGCTCAG AACTCAGGAT 
ATAAAAACTG TTACAAAGCC TGAACTGAAA TCAGAAGAAC CTATACGCCC GAGACTTCCA 
CCGCTAAAAT TCAATGCTAA CAAACCGTCT GGAGAATTTT TATTATCGCT CCAACGGGAG 
AAAGAGTTAA TGGAAGAGGC AAGAAAAAAT GGTTATGATG GAAGAACACA CAATCCTGAT 
GGGACTCCTC GAGAAATCAA AAAAGATTTC CTCGACACTG TTTGTCCACA AGCTGCAGCG 
CCAGAAGTTG TGATCAAAGA GAAAATCATA AACAACGATA CTTATACGCT GATGGGTCCA 
GCCGACTGGG GAAAACTGGA AGATATTGTC AAAGATGATT ACTCGGACAG TGGGGACAGT 
TGTTATTCAT CAAGAAGAGG AACCGGTAGT CAACCGACCC GACCGGCCGC TTCTCACCTC 
GCATGTCAAA TGCAAAACCG AACACAGTCA TTTGGAGCTA AACAACAAAC ATTTCAAAAC 
CGACTTCCGC CTACTGTAAA TCTGCCTGAT TCTGAAAGAA AAATTTCGGC TGCCAACCAA 
GGAACGTCAA ACCAACTCGA CCTTCCACAA GAAGATCCAA GAAAAAGAGC ATTCTCATTG 
GGAAGTAAAA ACTTTTTCAA CCTGATAGGA TTGAATGACT TTCGTCGCCT TGTAAGCAAG 
CGTCATCGAA CATCATCTCC AAACCACACC TCTACTTCTG GTATCTCGTT AAACTCATCT 
AACGCATCCC CGTCAGCAAG TAGTAATTTT TTGGCTTCTT CTGAATATTT AGAACATGCA 
AGAACTGATA GTTTTGGGTC CGCAAGAAGT AGTCCAAAAA GTCTACACAC CCAGCGAACC 
AGTTCACCGA AAAGAAGATC GGACGAGGAT CTGATCTCAA TTGACTTTTC AAGATTAGGT 
AAAAATTCTG CATCAGATAC AAAACGATTT CCTTTTGGTG GAGGAGGACC AGGAGGGTCC 
TTTGATTATG ATAGAGAAAA ACGGGAAAAG GAAGATAATA ATAGACGAGA CCGAGAAAAA 
GCAGCAATGG ATTTGAAAAG AAAAGAAGAA TGGGAAGCGA GAGAATTAGC GGAAAAGAAG 
AGGGAGTTGG AACGGAAAAT TCAAGCAAAG AAAGATAGAA AATTGGAGAA GGGAAGAGGG 
AAAGATAGAG AAAAGGATAA AGACCACGAT CATGATAAGG AGTTTCGTCC AAAAGCGGAC 
AGTGGAATTG CTGATTGTAC GCCCAGTTCA AGTTTTTCTG GGAAGAAAGA TCACAAAAAT 
GATGGTTCTT CTGATTCTGC ATACATGGAC AAAGAAGGGT TGAAGTACTT GGCAGATATC 
AAAAAACGGA AAAAAGAGCA AGGTGCCATA GACACAACAA ATTCCTCCTC CTCCTCGTTA 
AGCACAATAG TTTCGATTGA AGATAACAAA GTGACCAGAA GAATCACGGA AACCGTTGAA 
ACAAAAGTTG TGACTGCGGA TCTAGCGAAA GCACTTGGTG CAATTGACCC GAATAGAAGA 
TCTTCAGCAT GCATTGAGAT AAATCGAAAA CCATCGATTT GTACAGCAAT AATGGAAGAA 
AGAGAAGGAG AAGCATCTGA GACCGATTCT AGGGGAGAAC CATCCGACAG GAAGACGACC 
GCGGCCCCTG TAACTCGGAA ACCTGCATCG ACTGGCAGCA GCATGAGCCC GTTCAGAAGA 
CTTAAATTTC TCAGCTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ist-1 ( NM_001373346.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360716.1 Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Price & Availability↑

CloneID	OCa851130
Clone ID Related Accession (Same CDS sequence)	NM_001373346.1
Accession Version	NM_001373346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Insulin receptor SubsTrate homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284).

ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA CCGAAAAATG ACGGAGAGTA CGTTGAGACA 
ATGTTTGACG CCACGGAAGA GAAGAAACAA GAAACGATTG CGCCAAAACA GGAAGAAACT 
GTAAAGAAAT TATCAATGGC AGCAAGAGAG GAGAGAATAC GCGAAAGTAG ACAACAAGGG 
CAACTGAAAG AAAAAAGGCT GAAAAATCCA GATTCAAAAG GTGATACAAG TCACGATAAG 
CCGACCAGAG AAACATGGAA GCCGTTAGCC GTTGAAGACT TACCAAAGGA TGAAGATCCG 
GATGAATTTG GAATTCCAGA AGTCTACAAA TGTGGAAATT GTTTAGTAGG GTTTGCTCCA 
GTGAAAAAGA AAAAGCTAAT GTTTGTGACA CTCACGGAAC GATGTTTAGA ACTTCACGAG 
TCGGAGAAAT CCTATCGTGC GGGAAAAGCA GCAAAGCATA TGGTAGATCT CTCAATGTCT 
TTTAATGTGC ACAGTGAGCA TTACGATGCA AAACTAAAGA AATGCCTATG CTTGATGGGT 
CCAGACGAAA CAATATGCAT GAGACCGGAA GGAGGAATTC TAACCATTGA AGGTTGGCGA 
CGAGCAATTG TAAAACTAAT TCATGAATCA AGAAGAAGAA AAATGGACCG CGTTCCGAGA 
CCTGAAGATA TTTTTGATGC TGCATATGAT GTACGTGTTT GTCTATTTCC AAAAAACTTG 
GAAAAATACG TGGAAAGCTT GAAAACAGAC GGGTTCACAA ACATATGTAC TGTTGCCAAA 
GAGCTTTTAG GAAAGAAAAG ATTATGCCTT TATCCAAATA CACTTGCCAT TGTTGATCTC 
TGCATTGAAC CAACTGCCTA TGGTCTCCCA CCTGCCGGGT TTCCCCCATT CCGTGCATCA 
AGCATGTTCA TTTTGGAGCG AAATACGGTT GCTTACTACG GTTTTCGTGA AAACTATTTC 
TATGTACGAA TTGGAAAGGG AAGCCCCTAT CGAGGGTTTG AGTTATTGTT CCAAGTGGAC 
ACTAACGAAG TTTGTAAGGA AATTTACTCA CGTCTTAGAG CACTCGCTGA TCGTGATCTT 
GAAAACCGGA AACAAGAATC TATACGGAGA CCAGAAAGTG ACATGCATGG AATGGAATCC 
CTTTCTGTTC CCTCCCCGCT TTTGCATCGT ACCAAACTGT CCCTCGACTC GCCTGTTATA 
ACTCCCCGCG ACCGTCGCCT TATGTTGGGC CGTGAATGCC TATCGTTTGC CTCGCTTGAA 
AAAGATGATT CTGCGCCATC GTCTCCATTC GCCAACTACA ATCGTCCTCG TGGATCATTG 
GGGAATTTCC AGCTTGATCA CCTGTCTAGA TTTGACCAAC CTCGCGGATC AATTCATTCA 
CTGGGAAACA TTCCGATTGA TAGACCCCGT GTTTCTACTC TTCAAAGTGT GAAACCGGGC 
AAAGAAGAAA CCGGACTACG TGAAGCACTT CTTGAATCAT ATGGCCATTT AAGAAAAAAG 
AATTCAACAC AATCAGAGCG AGGAGAGATA GTTGTTCCGG AGCAAGAACG GAAAATGTCA 
GACAACAGGC CTCGGCTCAG AACTCAGGAT ATAAAAACTG TTACAAAGCC TGAACTGAAA 
TCAGAAGAAC CTATACGCCC GAGACTTCCA CCGCTAAAAT TCAATGCTAA CAAACCGTCT 
GGAGAATTTT TATTATCGCT CCAACGGGAG AAAGAGTTAA TGGAAGAGGC AAGAAAAAAT 
GGTTATGATG GAAGAACACA CAATCCTGAT GGGACTCCTC GAGAAATCAA AAAAGATTTC 
CTCGACACTG TTTGTCCACA AGCTGCAGCG CCAGAAGTTG TGATCAAAGA GAAAATCATA 
AACAACGATA CTTATACGCT GATGGGTCCA GCCGACTGGG GAAAACTGGA AGATATTGTC 
AAAGATGATT ACTCGGACAG TGGGGACAGT TGTTATTCAT CAAGAAGAGG AACCGGTAGT 
CAACCGACCC GACCGGCCGC TTCTCACCTC GCATGTCAAA TGCAAAACCG AACACAGTCA 
TTTGGAGCTA AACAACAAAC ATTTCAAAAC CGACTTCCGC CTACTGTAAA TCTGCCTGAT 
TCTGAAAGAA AAATTTCGGC TGCCAACCAA GGAACGTCAA ACCAACTCGA CCTTCCACAA 
GAAGATCCAA GAAAAAGAGC ATTCTCATTG GGAAGTAAAA ACTTTTTCAA CCTGATAGGA 
TTGAATGACT TTCGTCGCCT TGTAAGCAAG CGTCATCGAA CATCATCTCC AAACCACACC 
TCTACTTCTG GTATCTCGTT AAACTCATCT AACGCATCCC CGTCAGCAAG TAGTAATTTT 
TTGGCTTCTT CTGAATATTT AGAACATGCA AGAACTGATA GTTTTGGGTC CGCAAGAAGT 
AGTCCAAAAA GTCTACACAC CCAGCGAACC AGTTCACCGA AAAGAAGATC GGACGAGGAT 
CTGATCTCAA TTGACTTTTC AAGATTAGGT AAAAATTCTG CATCAGATAC AAAACGATTT 
CCTTTTGGTG GAGGAGGACC AGGAGGGTCC TTTGATTATG ATAGAGAAAA ACGGGAAAAG 
GAAGATAATA ATAGACGAGA CCGAGAAAAA GCAGCAATGG ATTTGAAAAG AAAAGAAGAA 
TGGGAAGCGA GAGAATTAGC GGAAAAGAAG AGGGAGTTGG AACGGAAAAT TCAAGCAAAG 
AAAGATAGAA AATTGGAGAA GGGAAGAGGG AAAGATAGAG AAAAGGATAA AGACCACGAT 
CATGATAAGG AGTTTCGTCC AAAAGCGGAC AGTGGAATTG CTGATTGTAC GCCCAGTTCA 
AGTTTTTCTG GGAAGAAAGA TCACAAAAAT GATGGTTCTT CTGATTCTGC ATACATGGAC 
AAAGAAGGGT TGAAGTACTT GGCAGATATC AAAAAACGGA AAAAAGAGCA AGGTGCCATA 
GACACAACAA ATTCCTCCTC CTCCTCGTTA AGCACAATAG TTTCGATTGA AGATAACAAA 
GTGACCAGAA GAATCACGGA AACCGTTGAA ACAAAAGTTG TGACTGCGGA TCTAGCGAAA 
GCACTTGGTG CAATTGACCC GAATAGAAGA TCTTCAGCAT GCATTGAGAT AAATCGAAAA 
CCATCGATTT GTACAGCAAT AATGGAAGAA AGAGAAGGAG AAGCATCTGA GACCGATTCT 
AGGGGAGAAC CATCCGACAG GAAGACGACC GCGGCCCCTG TAACTCGGAA ACCTGCATCG 
ACTGGCAGCA GCATGAGCCC GTTCAGAAGA CTTAAATTTC TCAGCTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360716.1
CDS	56..3352
Translation	MLPAEPTSGEPKNDGEYVETMFDATEEKKQETIAPKQEETVKKLSMAAREERIRESRQQGQLKEKRLKNPDSKGDTSHDKPTRETWKPLAVEDLPKDEDPDEFGIPEVYKCGNCLVGFAPVKKKKLMFVTLTERCLELHESEKSYRAGKAAKHMVDLSMSFNVHSEHYDAKLKKCLCLMGPDETICMRPEGGILTIEGWRRAIVKLIHESRRRKMDRVPRPEDIFDAAYDVRVCLFPKNLEKYVESLKTDGFTNICTVAKELLGKKRLCLYPNTLAIVDLCIEPTAYGLPPAGFPPFRASSMFILERNTVAYYGFRENYFYVRIGKGSPYRGFELLFQVDTNEVCKEIYSRLRALADRDLENRKQESIRRPESDMHGMESLSVPSPLLHRTKLSLDSPVITPRDRRLMLGRECLSFASLEKDDSAPSSPFANYNRPRGSLGNFQLDHLSRFDQPRGSIHSLGNIPIDRPRVSTLQSVKPGKEETGLREALLESYGHLRKKNSTQSERGEIVVPEQERKMSDNRPRLRTQDIKTVTKPELKSEEPIRPRLPPLKFNANKPSGEFLLSLQREKELMEEARKNGYDGRTHNPDGTPREIKKDFLDTVCPQAAAPEVVIKEKIINNDTYTLMGPADWGKLEDIVKDDYSDSGDSCYSSRRGTGSQPTRPAASHLACQMQNRTQSFGAKQQTFQNRLPPTVNLPDSERKISAANQGTSNQLDLPQEDPRKRAFSLGSKNFFNLIGLNDFRRLVSKRHRTSSPNHTSTSGISLNSSNASPSASSNFLASSEYLEHARTDSFGSARSSPKSLHTQRTSSPKRRSDEDLISIDFSRLGKNSASDTKRFPFGGGGPGGSFDYDREKREKEDNNRRDREKAAMDLKRKEEWEARELAEKKRELERKIQAKKDRKLEKGRGKDREKDKDHDHDKEFRPKADSGIADCTPSSSFSGKKDHKNDGSSDSAYMDKEGLKYLADIKKRKKEQGAIDTTNSSSSSLSTIVSIEDNKVTRRITETVETKVVTADLAKALGAIDPNRRSSACIEINRKPSICTAIMEEREGEASETDSRGEPSDRKTTAAPVTRKPASTGSSMSPFRRLKFLSFRK

Target ORF information:

RefSeq Version	NM_001373346.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Insulin receptor SubsTrate homolog (ist-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373346.1

ORF Insert Sequence:

ATGTTGCCCG CCGAGCCTAC ATCTGGTGAA CCGAAAAATG ACGGAGAGTA CGTTGAGACA 
ATGTTTGACG CCACGGAAGA GAAGAAACAA GAAACGATTG CGCCAAAACA GGAAGAAACT 
GTAAAGAAAT TATCAATGGC AGCAAGAGAG GAGAGAATAC GCGAAAGTAG ACAACAAGGG 
CAACTGAAAG AAAAAAGGCT GAAAAATCCA GATTCAAAAG GTGATACAAG TCACGATAAG 
CCGACCAGAG AAACATGGAA GCCGTTAGCC GTTGAAGACT TACCAAAGGA TGAAGATCCG 
GATGAATTTG GAATTCCAGA AGTCTACAAA TGTGGAAATT GTTTAGTAGG GTTTGCTCCA 
GTGAAAAAGA AAAAGCTAAT GTTTGTGACA CTCACGGAAC GATGTTTAGA ACTTCACGAG 
TCGGAGAAAT CCTATCGTGC GGGAAAAGCA GCAAAGCATA TGGTAGATCT CTCAATGTCT 
TTTAATGTGC ACAGTGAGCA TTACGATGCA AAACTAAAGA AATGCCTATG CTTGATGGGT 
CCAGACGAAA CAATATGCAT GAGACCGGAA GGAGGAATTC TAACCATTGA AGGTTGGCGA 
CGAGCAATTG TAAAACTAAT TCATGAATCA AGAAGAAGAA AAATGGACCG CGTTCCGAGA 
CCTGAAGATA TTTTTGATGC TGCATATGAT GTACGTGTTT GTCTATTTCC AAAAAACTTG 
GAAAAATACG TGGAAAGCTT GAAAACAGAC GGGTTCACAA ACATATGTAC TGTTGCCAAA 
GAGCTTTTAG GAAAGAAAAG ATTATGCCTT TATCCAAATA CACTTGCCAT TGTTGATCTC 
TGCATTGAAC CAACTGCCTA TGGTCTCCCA CCTGCCGGGT TTCCCCCATT CCGTGCATCA 
AGCATGTTCA TTTTGGAGCG AAATACGGTT GCTTACTACG GTTTTCGTGA AAACTATTTC 
TATGTACGAA TTGGAAAGGG AAGCCCCTAT CGAGGGTTTG AGTTATTGTT CCAAGTGGAC 
ACTAACGAAG TTTGTAAGGA AATTTACTCA CGTCTTAGAG CACTCGCTGA TCGTGATCTT 
GAAAACCGGA AACAAGAATC TATACGGAGA CCAGAAAGTG ACATGCATGG AATGGAATCC 
CTTTCTGTTC CCTCCCCGCT TTTGCATCGT ACCAAACTGT CCCTCGACTC GCCTGTTATA 
ACTCCCCGCG ACCGTCGCCT TATGTTGGGC CGTGAATGCC TATCGTTTGC CTCGCTTGAA 
AAAGATGATT CTGCGCCATC GTCTCCATTC GCCAACTACA ATCGTCCTCG TGGATCATTG 
GGGAATTTCC AGCTTGATCA CCTGTCTAGA TTTGACCAAC CTCGCGGATC AATTCATTCA 
CTGGGAAACA TTCCGATTGA TAGACCCCGT GTTTCTACTC TTCAAAGTGT GAAACCGGGC 
AAAGAAGAAA CCGGACTACG TGAAGCACTT CTTGAATCAT ATGGCCATTT AAGAAAAAAG 
AATTCAACAC AATCAGAGCG AGGAGAGATA GTTGTTCCGG AGCAAGAACG GAAAATGTCA 
GACAACAGGC CTCGGCTCAG AACTCAGGAT ATAAAAACTG TTACAAAGCC TGAACTGAAA 
TCAGAAGAAC CTATACGCCC GAGACTTCCA CCGCTAAAAT TCAATGCTAA CAAACCGTCT 
GGAGAATTTT TATTATCGCT CCAACGGGAG AAAGAGTTAA TGGAAGAGGC AAGAAAAAAT 
GGTTATGATG GAAGAACACA CAATCCTGAT GGGACTCCTC GAGAAATCAA AAAAGATTTC 
CTCGACACTG TTTGTCCACA AGCTGCAGCG CCAGAAGTTG TGATCAAAGA GAAAATCATA 
AACAACGATA CTTATACGCT GATGGGTCCA GCCGACTGGG GAAAACTGGA AGATATTGTC 
AAAGATGATT ACTCGGACAG TGGGGACAGT TGTTATTCAT CAAGAAGAGG AACCGGTAGT 
CAACCGACCC GACCGGCCGC TTCTCACCTC GCATGTCAAA TGCAAAACCG AACACAGTCA 
TTTGGAGCTA AACAACAAAC ATTTCAAAAC CGACTTCCGC CTACTGTAAA TCTGCCTGAT 
TCTGAAAGAA AAATTTCGGC TGCCAACCAA GGAACGTCAA ACCAACTCGA CCTTCCACAA 
GAAGATCCAA GAAAAAGAGC ATTCTCATTG GGAAGTAAAA ACTTTTTCAA CCTGATAGGA 
TTGAATGACT TTCGTCGCCT TGTAAGCAAG CGTCATCGAA CATCATCTCC AAACCACACC 
TCTACTTCTG GTATCTCGTT AAACTCATCT AACGCATCCC CGTCAGCAAG TAGTAATTTT 
TTGGCTTCTT CTGAATATTT AGAACATGCA AGAACTGATA GTTTTGGGTC CGCAAGAAGT 
AGTCCAAAAA GTCTACACAC CCAGCGAACC AGTTCACCGA AAAGAAGATC GGACGAGGAT 
CTGATCTCAA TTGACTTTTC AAGATTAGGT AAAAATTCTG CATCAGATAC AAAACGATTT 
CCTTTTGGTG GAGGAGGACC AGGAGGGTCC TTTGATTATG ATAGAGAAAA ACGGGAAAAG 
GAAGATAATA ATAGACGAGA CCGAGAAAAA GCAGCAATGG ATTTGAAAAG AAAAGAAGAA 
TGGGAAGCGA GAGAATTAGC GGAAAAGAAG AGGGAGTTGG AACGGAAAAT TCAAGCAAAG 
AAAGATAGAA AATTGGAGAA GGGAAGAGGG AAAGATAGAG AAAAGGATAA AGACCACGAT 
CATGATAAGG AGTTTCGTCC AAAAGCGGAC AGTGGAATTG CTGATTGTAC GCCCAGTTCA 
AGTTTTTCTG GGAAGAAAGA TCACAAAAAT GATGGTTCTT CTGATTCTGC ATACATGGAC 
AAAGAAGGGT TGAAGTACTT GGCAGATATC AAAAAACGGA AAAAAGAGCA AGGTGCCATA 
GACACAACAA ATTCCTCCTC CTCCTCGTTA AGCACAATAG TTTCGATTGA AGATAACAAA 
GTGACCAGAA GAATCACGGA AACCGTTGAA ACAAAAGTTG TGACTGCGGA TCTAGCGAAA 
GCACTTGGTG CAATTGACCC GAATAGAAGA TCTTCAGCAT GCATTGAGAT AAATCGAAAA 
CCATCGATTT GTACAGCAAT AATGGAAGAA AGAGAAGGAG AAGCATCTGA GACCGATTCT 
AGGGGAGAAC CATCCGACAG GAAGACGACC GCGGCCCCTG TAACTCGGAA ACCTGCATCG 
ACTGGCAGCA GCATGAGCCC GTTCAGAAGA CTTAAATTTC TCAGCTTCAG AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ist-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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