nnt-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nnt-1
Entrez Gene ID	180884
Full Name	Nicotinamide Nucleotide Transhydrogenase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_076627.3	NP_509028.1	Nicotinamide Nucleotide Transhydrogenase
NM_076627.5	NP_509028.1	Nicotinamide Nucleotide Transhydrogenase

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003167	XP_312859.4
Xenopus tropicalis (tropical clawed frog)	nnt	NP_001121474.1
Homo sapiens (human)	NNT	NP_036475.3
Canis lupus familiaris (dog)	NNT	XP_536481.3
Neurospora crassa	NCU01140	XP_961047.2
Pan troglodytes (chimpanzee)	NNT	XP_517776.2
Danio rerio (zebrafish)	nnt	NP_999921.1
Caenorhabditis elegans (roundworm)	nnt-1	NP_509028.1
Magnaporthe oryzae (rice blast fungus)	MGG_07539	XP_003711452.1
Bos taurus (cattle)	NNT	NP_776368.1
	NNT	XP_002804397.1
Rattus norvegicus (Norway rat)	Nnt	NP_001013175.1
Gallus gallus (chicken)	NNT	XP_003642984.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nnt-1 is important in the defense against mitochondrial oxidative stress
Title: A Caenorhabditis elegans mutant lacking functional nicotinamide nucleotide transhydrogenase displays increased sensitivity to oxidative stress.

evidence for expression in the gut, the pharyngeal-intestinal valve and certain neurons
Title: Expression of proton-pumping nicotinamide nucleotide transhydrogenase in mouse, human brain and C elegans.

The following nnt-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nnt-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349366	NM_076627.5 Latest version!	Caenorhabditis elegans Nicotinamide Nucleotide Transhydrogenase (nnt-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa349366	NM_076627.3	Caenorhabditis elegans Nicotinamide Nucleotide Transhydrogenase (nnt-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nnt-1 ( NM_076627.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509028.1 Caenorhabditis elegans Nicotinamide Nucleotide Transhydrogenase (nnt-1), partial mRNA.

Price & Availability↑

CloneID	OCa349366
Clone ID Related Accession (Same CDS sequence)	NM_076627.3 , NM_076627.5
Accession Version	NM_076627.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Nicotinamide Nucleotide Transhydrogenase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_076627.2. COMPLETENESS: incomplete on both ends.

ATGGTCATTC TTCGCTCAAA AATCACCAGA CTCTATTGGT TTCGATTGTT TTCAAATGGA 
CAGAAGAAAA TTGAATATTC CAAGTTAAAA GTTGCAGTTC CTAAAGAAAT ATTCCCAGGA 
GAAAAAAGAG TTTCACTCAG TCCAAACGGA GTTGCTCTTC TCAAGAAGAA CGGAATTTCA 
GTTTTGATTG AGGAAAATGC AGGAGTGCTC GCGGGCTACT CAAACGAGGA ATATGTCAGA 
AGCGGAGCAG ATGTGGGAAA ACATAATGAA GTTTTTAACA CAGATATTAT GTTGAAAGTC 
CGACCACCTA CGGAAAATGA AGTCTCTAAA CTTAAAAGCG GGTGCACATT GATTTCTTTC 
ATACATCCAG GACAAAATCA AGCTCTACTT GATTCACTGA CTAAAACCGA CAAGACCGTT 
TTTGCAATGG ATTGTGTGCC CAGAATTAGC CGAGCACAAG TATTTGATGC GTTATCCTCA 
ATGGCAAATA TCGCTGGATA CCGTGCAGTT ATTGAGGCTG CCAACCATTT TGGAAGATTC 
TTTACTGGGC AGATTACTGC TGCTGGTAAA GTTCCACCTG CAAAAGTACT TGTAATTGGT 
GGGGGAGTAG CCGGATTAAG CGCAATTGGA ACATCACGAG GAATGGGAGC AGTTGTGAGA 
GGATTTGATA CACGTGCTGC TGTCAAGGAG CACGTCGAAT CACTAGGTGC TCAATTTCTG 
ACCGTAAATG TGAAAGAAGA CGGAGAAGGC GGCGGTGGCT ATGCCAAAGA AATGAGCAAA 
GAGTTTATCG ACGCTGAAAT GAAACTTTTT GCGGATCAGT GCAAGGATGT GGATATTATA 
ATTACGACAG CACTCATTCC GGGAAAAAAG GCACCCATTC TGATTACAGA GGAAATGATA 
AAAAGCATGA AACCAGGCAG TGTAGTAGTG GACCTAGCCG CAGAGTCCGG TGGAAATATT 
GCGACGACTC GTCCTGGGGA AGTTTATGTT AAACATGGTG TCACTCATAT TGGATTCACA 
GACTTGCCAT CTAGACTTCC AACTCAAAGC AGTGAGCTTT ACTCAAATAA CATTGCAAAA 
TTTCTTTTAC ATCTCGGAAA AGATAAGACA TTCTTTGTGA ACGAAGAGGA TGAAGTGGCT 
CGTGGAGCAT TGGTTGTTCG TGATGGTCAA ATGAAATGGC CACCACCGCC AATTAACTTC 
CCACCTCCAG CAGCTCCAAA GTCAGATAAA CCATCTGAAA ATACCGCTTT GGTTCCATTA 
ACCCCATTCA GAAAAACTGC GAATCAGACG TTATTGTTGA CATCAGGACT CGGCTCCGTT 
TCTCTTCTTG GAATAGCTGG AACCAATCCT CAGATATCCA GTATGTCGAC AACATTTGCG 
CTAGCAGGAT TAGTTGGTTA CCATACCGTA TGGGGAGTCA CACCAGCACT TCATTCTCCT 
CTGATGAGTG TTACAAATGC AATTTCTGGA ACAACTGCAG CTGGAGCTTT GTGTCTGATG 
GGTGGAGGAC TTATGCCACA AAACTCCGCA CAGACAATGG CTCTGTTGGC AACGTTCATT 
TCATCAGTGA ATATTGGAGG TGGTTTCCTG GTGACTAAAA GAATGTTGGA TATGTTCAAG 
AGGAAAGATG ATCCACCAGA GCACAATTAT TTGTTCTCAA TTCCTGCTGC AGTTTTCTTA 
GGAGGTTATG GTTACGGAGT ATATACAGCC GCTCCATTAA TCCATTCCTA CGCTTATCTT 
GGCTCATCGC TATGTTGTGT AGGAGCTCTT GCAGGGCTGT CATCACAAAG CACGGCTCGT 
GTTGGAAATG CGCTCGGAAT AATTGGTGTC ACTGGTGGTA TTGGAGCAAC TTTAGGGTTA 
TTACAGCCTG ATTTCAATAC ACTTTGTCAA ATGGGCGGTT CGGTTGCAAT GGGTTCTCTT 
ATAGGTCTAG GGATTGCAAA TCGTATCAAG GTCACAGATC TACCTCAATT AGTTGCTGCT 
TTTCATTCTT TTGTTGGGCT TGCGGCGACT CTGACGTGCC TTGCAAACTT TATTCAGGAA 
CACCCACATT TCCTTGAAGA CCCGTCAAAT GCTGCAGCAG CAAAACTGGC TCTCTTCCTT 
GGAACATACA TTGGAGGAGT GACATTTACT GGAAGTTTGA TGGCTTATGG AAAACTTCAG 
GGAATTCTTG CCTCTGCTCC AACATACCTT CCCGCTCGAC ATGTGCTCAA TGGAGCTTTG 
CTTGCGGGAA ACGTTGGAGC ATTAGGAACT TACATGTATT CTACTGATTT TGGAACTGGA 
ATGAGCATGC TTGGTGGAAC TGTTGGACTC TCCAGCTTGA TGGGCGTGAC GTTGACAATG 
GCAATTGGAG GCGCCGATAT GCCAATTGTA ATCACAGTGT TGAATTCCTA CAGTGGATGG 
GCACTTTGCG CAGAAGGATT CATGTTGGAC AACAGTTTGT TAACGGTTCT CGGTGCGCTT 
ATTGGAAGTT CAGGAGCAAT TTTGTCCCAT ATCATGTGCA AGGCTATGAA CCGCTCTCTT 
CTAAATGTGA TCTTGGGAGG AGTTGGAACC AAGTCAAAAG GAACCGGTGA AGCAAAAGCA 
ATTGAGGGGA CTGCAAAAGA AATTGCACCA GTAGAAACTG CTGACATGCT TCTCAATGCT 
CGTTCAGTCA TCATCATTCC TGGATATGGT CTGTGTGCAG CTCAGGCACA ATATCCCATC 
GCTCAACTTG TCAAAGAATT GCAACAGAGA GGTGTTCGTG TCAGATTTGC AATTCATCCA 
GTTGCAGGAA GAATGCCGGG CCAGTTGAAC GTGCTTCTTG CGGAAGCAGG AGTGCCATAT 
GATATTGTGG AAGAAATGGA AGAAATAAAT GAAGACTTCA AAGAAACCGA TGTTGCACTT 
GTGATTGGTT CGAATGATAC CATCAATTCA GCGGCGGAAG ATGATCCGAA TAGTTCGATT 
GCAGGAATGC CCGTGTTGCG TGTCTGGAAT TCGAAGCAGG TCATTATCGT AAAAAGAACA 
CTCGGAACCG GTTACGCTGC AGTCGACAAT CCCGTGTTCT TTAATGAGAA CACTCAGATG 
TTACTAGGAG ATGCGAAAAA GATGAGCGAG AAACTTCTCG AAGAAGTGAA ATCGAAACCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509028.1
CDS	1..3126
Translation	MVILRSKITRLYWFRLFSNGQKKIEYSKLKVAVPKEIFPGEKRVSLSPNGVALLKKNGISVLIEENAGVLAGYSNEEYVRSGADVGKHNEVFNTDIMLKVRPPTENEVSKLKSGCTLISFIHPGQNQALLDSLTKTDKTVFAMDCVPRISRAQVFDALSSMANIAGYRAVIEAANHFGRFFTGQITAAGKVPPAKVLVIGGGVAGLSAIGTSRGMGAVVRGFDTRAAVKEHVESLGAQFLTVNVKEDGEGGGGYAKEMSKEFIDAEMKLFADQCKDVDIIITTALIPGKKAPILITEEMIKSMKPGSVVVDLAAESGGNIATTRPGEVYVKHGVTHIGFTDLPSRLPTQSSELYSNNIAKFLLHLGKDKTFFVNEEDEVARGALVVRDGQMKWPPPPINFPPPAAPKSDKPSENTALVPLTPFRKTANQTLLLTSGLGSVSLLGIAGTNPQISSMSTTFALAGLVGYHTVWGVTPALHSPLMSVTNAISGTTAAGALCLMGGGLMPQNSAQTMALLATFISSVNIGGGFLVTKRMLDMFKRKDDPPEHNYLFSIPAAVFLGGYGYGVYTAAPLIHSYAYLGSSLCCVGALAGLSSQSTARVGNALGIIGVTGGIGATLGLLQPDFNTLCQMGGSVAMGSLIGLGIANRIKVTDLPQLVAAFHSFVGLAATLTCLANFIQEHPHFLEDPSNAAAAKLALFLGTYIGGVTFTGSLMAYGKLQGILASAPTYLPARHVLNGALLAGNVGALGTYMYSTDFGTGMSMLGGTVGLSSLMGVTLTMAIGGADMPIVITVLNSYSGWALCAEGFMLDNSLLTVLGALIGSSGAILSHIMCKAMNRSLLNVILGGVGTKSKGTGEAKAIEGTAKEIAPVETADMLLNARSVIIIPGYGLCAAQAQYPIAQLVKELQQRGVRVRFAIHPVAGRMPGQLNVLLAEAGVPYDIVEEMEEINEDFKETDVALVIGSNDTINSAAEDDPNSSIAGMPVLRVWNSKQVIIVKRTLGTGYAAVDNPVFFNENTQMLLGDAKKMSEKLLEEVKSKPM

Target ORF information:

RefSeq Version	NM_076627.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nicotinamide Nucleotide Transhydrogenase (nnt-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_076627.3

ORF Insert Sequence:

ATGGTCATTC TTCGCTCAAA AATCACCAGA CTCTATTGGT TTCGATTGTT TTCAAATGGA 
CAGAAGAAAA TTGAATATTC CAAGTTAAAA GTTGCAGTTC CTAAAGAAAT ATTCCCAGGA 
GAAAAAAGAG TTTCACTCAG TCCAAACGGA GTTGCTCTTC TCAAGAAGAA CGGAATTTCA 
GTTTTGATTG AGGAAAATGC AGGAGTGCTC GCGGGCTACT CAAACGAGGA ATATGTCAGA 
AGCGGAGCAG ATGTGGGAAA ACATAATGAA GTTTTTAACA CAGATATTAT GTTGAAAGTC 
CGACCACCTA CGGAAAATGA AGTCTCTAAA CTTAAAAGCG GGTGCACATT GATTTCTTTC 
ATACATCCAG GACAAAATCA AGCTCTACTT GATTCACTGA CTAAAACCGA CAAGACCGTT 
TTTGCAATGG ATTGTGTGCC CAGAATTAGC CGAGCACAAG TATTTGATGC GTTATCCTCA 
ATGGCAAATA TCGCTGGATA CCGTGCAGTT ATTGAGGCTG CCAACCATTT TGGAAGATTC 
TTTACTGGGC AGATTACTGC TGCTGGTAAA GTTCCACCTG CAAAAGTACT TGTAATTGGT 
GGGGGAGTAG CCGGATTAAG CGCAATTGGA ACATCACGAG GAATGGGAGC AGTTGTGAGA 
GGATTTGATA CACGTGCTGC TGTCAAGGAG CACGTCGAAT CACTAGGTGC TCAATTTCTG 
ACCGTAAATG TGAAAGAAGA CGGAGAAGGC GGCGGTGGCT ATGCCAAAGA AATGAGCAAA 
GAGTTTATCG ACGCTGAAAT GAAACTTTTT GCGGATCAGT GCAAGGATGT GGATATTATA 
ATTACGACAG CACTCATTCC GGGAAAAAAG GCACCCATTC TGATTACAGA GGAAATGATA 
AAAAGCATGA AACCAGGCAG TGTAGTAGTG GACCTAGCCG CAGAGTCCGG TGGAAATATT 
GCGACGACTC GTCCTGGGGA AGTTTATGTT AAACATGGTG TCACTCATAT TGGATTCACA 
GACTTGCCAT CTAGACTTCC AACTCAAAGC AGTGAGCTTT ACTCAAATAA CATTGCAAAA 
TTTCTTTTAC ATCTCGGAAA AGATAAGACA TTCTTTGTGA ACGAAGAGGA TGAAGTGGCT 
CGTGGAGCAT TGGTTGTTCG TGATGGTCAA ATGAAATGGC CACCACCGCC AATTAACTTC 
CCACCTCCAG CAGCTCCAAA GTCAGATAAA CCATCTGAAA ATACCGCTTT GGTTCCATTA 
ACCCCATTCA GAAAAACTGC GAATCAGACG TTATTGTTGA CATCAGGACT CGGCTCCGTT 
TCTCTTCTTG GAATAGCTGG AACCAATCCT CAGATATCCA GTATGTCGAC AACATTTGCG 
CTAGCAGGAT TAGTTGGTTA CCATACCGTA TGGGGAGTCA CACCAGCACT TCATTCTCCT 
CTGATGAGTG TTACAAATGC AATTTCTGGA ACAACTGCAG CTGGAGCTTT GTGTCTGATG 
GGTGGAGGAC TTATGCCACA AAACTCCGCA CAGACAATGG CTCTGTTGGC AACGTTCATT 
TCATCAGTGA ATATTGGAGG TGGTTTCCTG GTGACTAAAA GAATGTTGGA TATGTTCAAG 
AGGAAAGATG ATCCACCAGA GCACAATTAT TTGTTCTCAA TTCCTGCTGC AGTTTTCTTA 
GGAGGTTATG GTTACGGAGT ATATACAGCC GCTCCATTAA TCCATTCCTA CGCTTATCTT 
GGCTCATCGC TATGTTGTGT AGGAGCTCTT GCAGGGCTGT CATCACAAAG CACGGCTCGT 
GTTGGAAATG CGCTCGGAAT AATTGGTGTC ACTGGTGGTA TTGGAGCAAC TTTAGGGTTA 
TTACAGCCTG ATTTCAATAC ACTTTGTCAA ATGGGCGGTT CGGTTGCAAT GGGTTCTCTT 
ATAGGTCTAG GGATTGCAAA TCGTATCAAG GTCACAGATC TACCTCAATT AGTTGCTGCT 
TTTCATTCTT TTGTTGGGCT TGCGGCGACT CTGACGTGCC TTGCAAACTT TATTCAGGAA 
CACCCACATT TCCTTGAAGA CCCGTCAAAT GCTGCAGCAG CAAAACTGGC TCTCTTCCTT 
GGAACATACA TTGGAGGAGT GACATTTACT GGAAGTTTGA TGGCTTATGG AAAACTTCAG 
GGAATTCTTG CCTCTGCTCC AACATACCTT CCCGCTCGAC ATGTGCTCAA TGGAGCTTTG 
CTTGCGGGAA ACGTTGGAGC ATTAGGAACT TACATGTATT CTACTGATTT TGGAACTGGA 
ATGAGCATGC TTGGTGGAAC TGTTGGACTC TCCAGCTTGA TGGGCGTGAC GTTGACAATG 
GCAATTGGAG GCGCCGATAT GCCAATTGTA ATCACAGTGT TGAATTCCTA CAGTGGATGG 
GCACTTTGCG CAGAAGGATT CATGTTGGAC AACAGTTTGT TAACGGTTCT CGGTGCGCTT 
ATTGGAAGTT CAGGAGCAAT TTTGTCCCAT ATCATGTGCA AGGCTATGAA CCGCTCTCTT 
CTAAATGTGA TCTTGGGAGG AGTTGGAACC AAGTCAAAAG GAACCGGTGA AGCAAAAGCA 
ATTGAGGGGA CTGCAAAAGA AATTGCACCA GTAGAAACTG CTGACATGCT TCTCAATGCT 
CGTTCAGTCA TCATCATTCC TGGATATGGT CTGTGTGCAG CTCAGGCACA ATATCCCATC 
GCTCAACTTG TCAAAGAATT GCAACAGAGA GGTGTTCGTG TCAGATTTGC AATTCATCCA 
GTTGCAGGAA GAATGCCGGG CCAGTTGAAC GTGCTTCTTG CGGAAGCAGG AGTGCCATAT 
GATATTGTGG AAGAAATGGA AGAAATAAAT GAAGACTTCA AAGAAACCGA TGTTGCACTT 
GTGATTGGTT CGAATGATAC CATCAATTCA GCGGCGGAAG ATGATCCGAA TAGTTCGATT 
GCAGGAATGC CCGTGTTGCG TGTCTGGAAT TCGAAGCAGG TCATTATCGT AAAAAGAACA 
CTCGGAACCG GTTACGCTGC AGTCGACAAT CCCGTGTTCT TTAATGAGAA CACTCAGATG 
TTACTAGGAG ATGCGAAAAA GATGAGCGAG AAACTTCTCG AAGAAGTGAA ATCGAAACCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nnt-1 ( NM_076627.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509028.1 Caenorhabditis elegans Nicotinamide Nucleotide Transhydrogenase (nnt-1), mRNA.

Price & Availability↑

CloneID	OCa349366
Clone ID Related Accession (Same CDS sequence)	NM_076627.3 , NM_076627.5
Accession Version	NM_076627.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Nicotinamide Nucleotide Transhydrogenase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_076627.4.

ATGGTCATTC TTCGCTCAAA AATCACCAGA CTCTATTGGT TTCGATTGTT TTCAAATGGA 
CAGAAGAAAA TTGAATATTC CAAGTTAAAA GTTGCAGTTC CTAAAGAAAT ATTCCCAGGA 
GAAAAAAGAG TTTCACTCAG TCCAAACGGA GTTGCTCTTC TCAAGAAGAA CGGAATTTCA 
GTTTTGATTG AGGAAAATGC AGGAGTGCTC GCGGGCTACT CAAACGAGGA ATATGTCAGA 
AGCGGAGCAG ATGTGGGAAA ACATAATGAA GTTTTTAACA CAGATATTAT GTTGAAAGTC 
CGACCACCTA CGGAAAATGA AGTCTCTAAA CTTAAAAGCG GGTGCACATT GATTTCTTTC 
ATACATCCAG GACAAAATCA AGCTCTACTT GATTCACTGA CTAAAACCGA CAAGACCGTT 
TTTGCAATGG ATTGTGTGCC CAGAATTAGC CGAGCACAAG TATTTGATGC GTTATCCTCA 
ATGGCAAATA TCGCTGGATA CCGTGCAGTT ATTGAGGCTG CCAACCATTT TGGAAGATTC 
TTTACTGGGC AGATTACTGC TGCTGGTAAA GTTCCACCTG CAAAAGTACT TGTAATTGGT 
GGGGGAGTAG CCGGATTAAG CGCAATTGGA ACATCACGAG GAATGGGAGC AGTTGTGAGA 
GGATTTGATA CACGTGCTGC TGTCAAGGAG CACGTCGAAT CACTAGGTGC TCAATTTCTG 
ACCGTAAATG TGAAAGAAGA CGGAGAAGGC GGCGGTGGCT ATGCCAAAGA AATGAGCAAA 
GAGTTTATCG ACGCTGAAAT GAAACTTTTT GCGGATCAGT GCAAGGATGT GGATATTATA 
ATTACGACAG CACTCATTCC GGGAAAAAAG GCACCCATTC TGATTACAGA GGAAATGATA 
AAAAGCATGA AACCAGGCAG TGTAGTAGTG GACCTAGCCG CAGAGTCCGG TGGAAATATT 
GCGACGACTC GTCCTGGGGA AGTTTATGTT AAACATGGTG TCACTCATAT TGGATTCACA 
GACTTGCCAT CTAGACTTCC AACTCAAAGC AGTGAGCTTT ACTCAAATAA CATTGCAAAA 
TTTCTTTTAC ATCTCGGAAA AGATAAGACA TTCTTTGTGA ACGAAGAGGA TGAAGTGGCT 
CGTGGAGCAT TGGTTGTTCG TGATGGTCAA ATGAAATGGC CACCACCGCC AATTAACTTC 
CCACCTCCAG CAGCTCCAAA GTCAGATAAA CCATCTGAAA ATACCGCTTT GGTTCCATTA 
ACCCCATTCA GAAAAACTGC GAATCAGACG TTATTGTTGA CATCAGGACT CGGCTCCGTT 
TCTCTTCTTG GAATAGCTGG AACCAATCCT CAGATATCCA GTATGTCGAC AACATTTGCG 
CTAGCAGGAT TAGTTGGTTA CCATACCGTA TGGGGAGTCA CACCAGCACT TCATTCTCCT 
CTGATGAGTG TTACAAATGC AATTTCTGGA ACAACTGCAG CTGGAGCTTT GTGTCTGATG 
GGTGGAGGAC TTATGCCACA AAACTCCGCA CAGACAATGG CTCTGTTGGC AACGTTCATT 
TCATCAGTGA ATATTGGAGG TGGTTTCCTG GTGACTAAAA GAATGTTGGA TATGTTCAAG 
AGGAAAGATG ATCCACCAGA GCACAATTAT TTGTTCTCAA TTCCTGCTGC AGTTTTCTTA 
GGAGGTTATG GTTACGGAGT ATATACAGCC GCTCCATTAA TCCATTCCTA CGCTTATCTT 
GGCTCATCGC TATGTTGTGT AGGAGCTCTT GCAGGGCTGT CATCACAAAG CACGGCTCGT 
GTTGGAAATG CGCTCGGAAT AATTGGTGTC ACTGGTGGTA TTGGAGCAAC TTTAGGGTTA 
TTACAGCCTG ATTTCAATAC ACTTTGTCAA ATGGGCGGTT CGGTTGCAAT GGGTTCTCTT 
ATAGGTCTAG GGATTGCAAA TCGTATCAAG GTCACAGATC TACCTCAATT AGTTGCTGCT 
TTTCATTCTT TTGTTGGGCT TGCGGCGACT CTGACGTGCC TTGCAAACTT TATTCAGGAA 
CACCCACATT TCCTTGAAGA CCCGTCAAAT GCTGCAGCAG CAAAACTGGC TCTCTTCCTT 
GGAACATACA TTGGAGGAGT GACATTTACT GGAAGTTTGA TGGCTTATGG AAAACTTCAG 
GGAATTCTTG CCTCTGCTCC AACATACCTT CCCGCTCGAC ATGTGCTCAA TGGAGCTTTG 
CTTGCGGGAA ACGTTGGAGC ATTAGGAACT TACATGTATT CTACTGATTT TGGAACTGGA 
ATGAGCATGC TTGGTGGAAC TGTTGGACTC TCCAGCTTGA TGGGCGTGAC GTTGACAATG 
GCAATTGGAG GCGCCGATAT GCCAATTGTA ATCACAGTGT TGAATTCCTA CAGTGGATGG 
GCACTTTGCG CAGAAGGATT CATGTTGGAC AACAGTTTGT TAACGGTTCT CGGTGCGCTT 
ATTGGAAGTT CAGGAGCAAT TTTGTCCCAT ATCATGTGCA AGGCTATGAA CCGCTCTCTT 
CTAAATGTGA TCTTGGGAGG AGTTGGAACC AAGTCAAAAG GAACCGGTGA AGCAAAAGCA 
ATTGAGGGGA CTGCAAAAGA AATTGCACCA GTAGAAACTG CTGACATGCT TCTCAATGCT 
CGTTCAGTCA TCATCATTCC TGGATATGGT CTGTGTGCAG CTCAGGCACA ATATCCCATC 
GCTCAACTTG TCAAAGAATT GCAACAGAGA GGTGTTCGTG TCAGATTTGC AATTCATCCA 
GTTGCAGGAA GAATGCCGGG CCAGTTGAAC GTGCTTCTTG CGGAAGCAGG AGTGCCATAT 
GATATTGTGG AAGAAATGGA AGAAATAAAT GAAGACTTCA AAGAAACCGA TGTTGCACTT 
GTGATTGGTT CGAATGATAC CATCAATTCA GCGGCGGAAG ATGATCCGAA TAGTTCGATT 
GCAGGAATGC CCGTGTTGCG TGTCTGGAAT TCGAAGCAGG TCATTATCGT AAAAAGAACA 
CTCGGAACCG GTTACGCTGC AGTCGACAAT CCCGTGTTCT TTAATGAGAA CACTCAGATG 
TTACTAGGAG ATGCGAAAAA GATGAGCGAG AAACTTCTCG AAGAAGTGAA ATCGAAACCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509028.1
CDS	8..3133
Translation	MVILRSKITRLYWFRLFSNGQKKIEYSKLKVAVPKEIFPGEKRVSLSPNGVALLKKNGISVLIEENAGVLAGYSNEEYVRSGADVGKHNEVFNTDIMLKVRPPTENEVSKLKSGCTLISFIHPGQNQALLDSLTKTDKTVFAMDCVPRISRAQVFDALSSMANIAGYRAVIEAANHFGRFFTGQITAAGKVPPAKVLVIGGGVAGLSAIGTSRGMGAVVRGFDTRAAVKEHVESLGAQFLTVNVKEDGEGGGGYAKEMSKEFIDAEMKLFADQCKDVDIIITTALIPGKKAPILITEEMIKSMKPGSVVVDLAAESGGNIATTRPGEVYVKHGVTHIGFTDLPSRLPTQSSELYSNNIAKFLLHLGKDKTFFVNEEDEVARGALVVRDGQMKWPPPPINFPPPAAPKSDKPSENTALVPLTPFRKTANQTLLLTSGLGSVSLLGIAGTNPQISSMSTTFALAGLVGYHTVWGVTPALHSPLMSVTNAISGTTAAGALCLMGGGLMPQNSAQTMALLATFISSVNIGGGFLVTKRMLDMFKRKDDPPEHNYLFSIPAAVFLGGYGYGVYTAAPLIHSYAYLGSSLCCVGALAGLSSQSTARVGNALGIIGVTGGIGATLGLLQPDFNTLCQMGGSVAMGSLIGLGIANRIKVTDLPQLVAAFHSFVGLAATLTCLANFIQEHPHFLEDPSNAAAAKLALFLGTYIGGVTFTGSLMAYGKLQGILASAPTYLPARHVLNGALLAGNVGALGTYMYSTDFGTGMSMLGGTVGLSSLMGVTLTMAIGGADMPIVITVLNSYSGWALCAEGFMLDNSLLTVLGALIGSSGAILSHIMCKAMNRSLLNVILGGVGTKSKGTGEAKAIEGTAKEIAPVETADMLLNARSVIIIPGYGLCAAQAQYPIAQLVKELQQRGVRVRFAIHPVAGRMPGQLNVLLAEAGVPYDIVEEMEEINEDFKETDVALVIGSNDTINSAAEDDPNSSIAGMPVLRVWNSKQVIIVKRTLGTGYAAVDNPVFFNENTQMLLGDAKKMSEKLLEEVKSKPM

Target ORF information:

RefSeq Version	NM_076627.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Nicotinamide Nucleotide Transhydrogenase (nnt-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_076627.5

ORF Insert Sequence:

ATGGTCATTC TTCGCTCAAA AATCACCAGA CTCTATTGGT TTCGATTGTT TTCAAATGGA 
CAGAAGAAAA TTGAATATTC CAAGTTAAAA GTTGCAGTTC CTAAAGAAAT ATTCCCAGGA 
GAAAAAAGAG TTTCACTCAG TCCAAACGGA GTTGCTCTTC TCAAGAAGAA CGGAATTTCA 
GTTTTGATTG AGGAAAATGC AGGAGTGCTC GCGGGCTACT CAAACGAGGA ATATGTCAGA 
AGCGGAGCAG ATGTGGGAAA ACATAATGAA GTTTTTAACA CAGATATTAT GTTGAAAGTC 
CGACCACCTA CGGAAAATGA AGTCTCTAAA CTTAAAAGCG GGTGCACATT GATTTCTTTC 
ATACATCCAG GACAAAATCA AGCTCTACTT GATTCACTGA CTAAAACCGA CAAGACCGTT 
TTTGCAATGG ATTGTGTGCC CAGAATTAGC CGAGCACAAG TATTTGATGC GTTATCCTCA 
ATGGCAAATA TCGCTGGATA CCGTGCAGTT ATTGAGGCTG CCAACCATTT TGGAAGATTC 
TTTACTGGGC AGATTACTGC TGCTGGTAAA GTTCCACCTG CAAAAGTACT TGTAATTGGT 
GGGGGAGTAG CCGGATTAAG CGCAATTGGA ACATCACGAG GAATGGGAGC AGTTGTGAGA 
GGATTTGATA CACGTGCTGC TGTCAAGGAG CACGTCGAAT CACTAGGTGC TCAATTTCTG 
ACCGTAAATG TGAAAGAAGA CGGAGAAGGC GGCGGTGGCT ATGCCAAAGA AATGAGCAAA 
GAGTTTATCG ACGCTGAAAT GAAACTTTTT GCGGATCAGT GCAAGGATGT GGATATTATA 
ATTACGACAG CACTCATTCC GGGAAAAAAG GCACCCATTC TGATTACAGA GGAAATGATA 
AAAAGCATGA AACCAGGCAG TGTAGTAGTG GACCTAGCCG CAGAGTCCGG TGGAAATATT 
GCGACGACTC GTCCTGGGGA AGTTTATGTT AAACATGGTG TCACTCATAT TGGATTCACA 
GACTTGCCAT CTAGACTTCC AACTCAAAGC AGTGAGCTTT ACTCAAATAA CATTGCAAAA 
TTTCTTTTAC ATCTCGGAAA AGATAAGACA TTCTTTGTGA ACGAAGAGGA TGAAGTGGCT 
CGTGGAGCAT TGGTTGTTCG TGATGGTCAA ATGAAATGGC CACCACCGCC AATTAACTTC 
CCACCTCCAG CAGCTCCAAA GTCAGATAAA CCATCTGAAA ATACCGCTTT GGTTCCATTA 
ACCCCATTCA GAAAAACTGC GAATCAGACG TTATTGTTGA CATCAGGACT CGGCTCCGTT 
TCTCTTCTTG GAATAGCTGG AACCAATCCT CAGATATCCA GTATGTCGAC AACATTTGCG 
CTAGCAGGAT TAGTTGGTTA CCATACCGTA TGGGGAGTCA CACCAGCACT TCATTCTCCT 
CTGATGAGTG TTACAAATGC AATTTCTGGA ACAACTGCAG CTGGAGCTTT GTGTCTGATG 
GGTGGAGGAC TTATGCCACA AAACTCCGCA CAGACAATGG CTCTGTTGGC AACGTTCATT 
TCATCAGTGA ATATTGGAGG TGGTTTCCTG GTGACTAAAA GAATGTTGGA TATGTTCAAG 
AGGAAAGATG ATCCACCAGA GCACAATTAT TTGTTCTCAA TTCCTGCTGC AGTTTTCTTA 
GGAGGTTATG GTTACGGAGT ATATACAGCC GCTCCATTAA TCCATTCCTA CGCTTATCTT 
GGCTCATCGC TATGTTGTGT AGGAGCTCTT GCAGGGCTGT CATCACAAAG CACGGCTCGT 
GTTGGAAATG CGCTCGGAAT AATTGGTGTC ACTGGTGGTA TTGGAGCAAC TTTAGGGTTA 
TTACAGCCTG ATTTCAATAC ACTTTGTCAA ATGGGCGGTT CGGTTGCAAT GGGTTCTCTT 
ATAGGTCTAG GGATTGCAAA TCGTATCAAG GTCACAGATC TACCTCAATT AGTTGCTGCT 
TTTCATTCTT TTGTTGGGCT TGCGGCGACT CTGACGTGCC TTGCAAACTT TATTCAGGAA 
CACCCACATT TCCTTGAAGA CCCGTCAAAT GCTGCAGCAG CAAAACTGGC TCTCTTCCTT 
GGAACATACA TTGGAGGAGT GACATTTACT GGAAGTTTGA TGGCTTATGG AAAACTTCAG 
GGAATTCTTG CCTCTGCTCC AACATACCTT CCCGCTCGAC ATGTGCTCAA TGGAGCTTTG 
CTTGCGGGAA ACGTTGGAGC ATTAGGAACT TACATGTATT CTACTGATTT TGGAACTGGA 
ATGAGCATGC TTGGTGGAAC TGTTGGACTC TCCAGCTTGA TGGGCGTGAC GTTGACAATG 
GCAATTGGAG GCGCCGATAT GCCAATTGTA ATCACAGTGT TGAATTCCTA CAGTGGATGG 
GCACTTTGCG CAGAAGGATT CATGTTGGAC AACAGTTTGT TAACGGTTCT CGGTGCGCTT 
ATTGGAAGTT CAGGAGCAAT TTTGTCCCAT ATCATGTGCA AGGCTATGAA CCGCTCTCTT 
CTAAATGTGA TCTTGGGAGG AGTTGGAACC AAGTCAAAAG GAACCGGTGA AGCAAAAGCA 
ATTGAGGGGA CTGCAAAAGA AATTGCACCA GTAGAAACTG CTGACATGCT TCTCAATGCT 
CGTTCAGTCA TCATCATTCC TGGATATGGT CTGTGTGCAG CTCAGGCACA ATATCCCATC 
GCTCAACTTG TCAAAGAATT GCAACAGAGA GGTGTTCGTG TCAGATTTGC AATTCATCCA 
GTTGCAGGAA GAATGCCGGG CCAGTTGAAC GTGCTTCTTG CGGAAGCAGG AGTGCCATAT 
GATATTGTGG AAGAAATGGA AGAAATAAAT GAAGACTTCA AAGAAACCGA TGTTGCACTT 
GTGATTGGTT CGAATGATAC CATCAATTCA GCGGCGGAAG ATGATCCGAA TAGTTCGATT 
GCAGGAATGC CCGTGTTGCG TGTCTGGAAT TCGAAGCAGG TCATTATCGT AAAAAGAACA 
CTCGGAACCG GTTACGCTGC AGTCGACAAT CCCGTGTTCT TTAATGAGAA CACTCAGATG 
TTACTAGGAG ATGCGAAAAA GATGAGCGAG AAACTTCTCG AAGAAGTGAA ATCGAAACCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nnt-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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