unc-2 gene cDNA ORF clone, Caenorhabditis elegans

mRNA	Protein	Name
NM_001129704.1	NP_001123176.1	Uncharacterized protein
NM_001348589.1	NP_001335563.1	Uncharacterized protein
NM_001368497.1	NP_001355494.1	Uncharacterized protein
NM_001348591.3	NP_001335562.1	EF-hand domain-containing protein
NM_001368496.3	NP_001355462.1	EF-hand domain-containing protein
NM_001368495.3	NP_001355463.1	EF-hand domain-containing protein
NM_001368494.3	NP_001355464.1	EF-hand domain-containing protein
NM_001348590.1	NP_001335560.1	Uncharacterized protein
NM_001368495.1	NP_001355463.1	Uncharacterized protein
NM_171638.2	NP_741732.2	Uncharacterized protein
NM_001348596.1	NP_001335516.1	Uncharacterized protein
NM_001348597.1	NP_001335515.1	Uncharacterized protein
NM_001348596.3	NP_001335516.1	EF-hand domain-containing protein
NM_001368497.3	NP_001355494.1	EF-hand domain-containing protein
NM_001348597.2	NP_001335515.1	EF-hand domain-containing protein
NM_001029700.2	NP_001024871.1	Uncharacterized protein
NM_001348595.1	NP_001335572.1	Uncharacterized protein
NM_001348593.1	NP_001335573.1	Uncharacterized protein
NM_001348588.3	NP_001335561.1	EF-hand domain-containing protein
NM_001348593.3	NP_001335573.1	EF-hand domain-containing protein
NM_001348591.1	NP_001335562.1	Uncharacterized protein
NM_001348594.1	NP_001335570.1	Uncharacterized protein
NM_001348592.1	NP_001335571.1	Uncharacterized protein
NM_001348595.3	NP_001335572.1	EF-hand domain-containing protein
NM_001348588.1	NP_001335561.1	Uncharacterized protein
NM_001368496.1	NP_001355462.1	Uncharacterized protein
NM_001368494.1	NP_001355464.1	Uncharacterized protein
NM_001348590.3	NP_001335560.1	EF-hand domain-containing protein
NM_001348589.3	NP_001335563.1	EF-hand domain-containing protein
NM_171638.3	NP_741732.2	EF-hand domain-containing protein
NM_001348594.3	NP_001335570.1	EF-hand domain-containing protein
NM_001348592.3	NP_001335571.1	EF-hand domain-containing protein

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa355210	NM_001029700.2 Latest version!	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OCa355200	NM_001348588.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa355201	NM_001348589.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa355209	NM_001348596.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa355211	NM_001348597.2 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCa698996	NM_001368497.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa355199	NM_001129704.1 Latest version!	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355202	NM_001348590.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355203	NM_001348591.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355204	NM_001348592.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355205	NM_001348593.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355206	NM_001348594.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355207	NM_001348595.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355208	NM_171638.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa698993	NM_001368494.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa698994	NM_001368495.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa698995	NM_001368496.3 Latest version!	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa698994	NM_001368495.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355209	NM_001348596.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa698996	NM_001368497.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa355211	NM_001348597.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa355205	NM_001348593.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355203	NM_001348591.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355207	NM_001348595.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355200	NM_001348588.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa698995	NM_001368496.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa698993	NM_001368494.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa355202	NM_001348590.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355201	NM_001348589.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa355208	NM_171638.2	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355206	NM_001348594.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa355204	NM_001348592.1	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

unc-2 ( NM_001029700.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024871.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Price & Availability↑

CloneID	OCa355210
Clone ID Related Accession (Same CDS sequence)	NM_001029700.2
Accession Version	NM_001029700.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001029700.1. COMPLETENESS: incomplete on both ends.

ATGGCCCGCG CGTCACCGCC GAGTCCTGCT GAACGATACG GTCATCCACC CAGATATCGC 
ACCGAATCGC CTCCATCTTC GAGATCGGAG TACCAGATGT CAATTAGAGA CCCGATAATT 
AGGCGTAATC GCTATAATAC AATGGAACAC TCGCGATCTT CCCACGATCC ACAATATCAT 
CAGGATCAAC AACAACAACA ACAACCACAT CACCAGCAAC ACTCACAACA TTTGCAACAT 
TCACACCACA AAACGTACCA AAATCATAAT CAATATTCAA GAAGTCCAAT CTATTCCGAT 
GATTCGAGTG TTGCTGAAAG TTATCGAAGA GAGCGAGAAT TCCGAAGATA TCAAGACTCC 
ACTCCACAAG ATGTCAGCGA GGACGACGAC CCGATGCCTA CGGCGGTCCG AGCGCGCCGA 
TTACCACTGA TTTCCACAAT GCCCACGCAC TACGAGTCGG CCTACCAGCC ATCATCATAT 
AACCAGCATC TCAATGATTC ATATGGTTTA GGAACCGGGT ACCAACGAGA TTATCACACT 
AGTCATTCAC ACTCGCACCA TCCCACCTCA CAACAACAAC AACATCAGCC CATGTACAGC 
ACGTCACCAC TAATATCACC GAGATCGAGT CATAGCTACT ACACGCCGAG GAGCTCGCAA 
TACTATGAAA TACCATCGCC GAGTCCAGAT ATTTATCCAT CGTATCGTGG ATCGGCATCA 
CCTCGCAGGT ACCCCACCAG TACCGTCGTA GTGGCTCCAG ATCGAGAAGG TTCGAGCGCT 
CGCGTCATTC AAGCTCAGCC TGGCTCGATA CCACTATCTG ACTCGGAAAC AGAAGATGAT 
CCTCGATGGG CAATTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024871.1
CDS	1..861
Translation	MARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001029700.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029700.2

ORF Insert Sequence:

ATGGCCCGCG CGTCACCGCC GAGTCCTGCT GAACGATACG GTCATCCACC CAGATATCGC 
ACCGAATCGC CTCCATCTTC GAGATCGGAG TACCAGATGT CAATTAGAGA CCCGATAATT 
AGGCGTAATC GCTATAATAC AATGGAACAC TCGCGATCTT CCCACGATCC ACAATATCAT 
CAGGATCAAC AACAACAACA ACAACCACAT CACCAGCAAC ACTCACAACA TTTGCAACAT 
TCACACCACA AAACGTACCA AAATCATAAT CAATATTCAA GAAGTCCAAT CTATTCCGAT 
GATTCGAGTG TTGCTGAAAG TTATCGAAGA GAGCGAGAAT TCCGAAGATA TCAAGACTCC 
ACTCCACAAG ATGTCAGCGA GGACGACGAC CCGATGCCTA CGGCGGTCCG AGCGCGCCGA 
TTACCACTGA TTTCCACAAT GCCCACGCAC TACGAGTCGG CCTACCAGCC ATCATCATAT 
AACCAGCATC TCAATGATTC ATATGGTTTA GGAACCGGGT ACCAACGAGA TTATCACACT 
AGTCATTCAC ACTCGCACCA TCCCACCTCA CAACAACAAC AACATCAGCC CATGTACAGC 
ACGTCACCAC TAATATCACC GAGATCGAGT CATAGCTACT ACACGCCGAG GAGCTCGCAA 
TACTATGAAA TACCATCGCC GAGTCCAGAT ATTTATCCAT CGTATCGTGG ATCGGCATCA 
CCTCGCAGGT ACCCCACCAG TACCGTCGTA GTGGCTCCAG ATCGAGAAGG TTCGAGCGCT 
CGCGTCATTC AAGCTCAGCC TGGCTCGATA CCACTATCTG ACTCGGAAAC AGAAGATGAT 
CCTCGATGGG CAATTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348588.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335561.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Price & Availability↑

CloneID	OCa355200
Clone ID Related Accession (Same CDS sequence)	NM_001348588.3 , NM_001348588.1
Accession Version	NM_001348588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348588.2. COMPLETENESS: incomplete on the 5' end.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335561.1
CDS	1..5949
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348588.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348588.3

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348588.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335561.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355200
Clone ID Related Accession (Same CDS sequence)	NM_001348588.3 , NM_001348588.1
Accession Version	NM_001348588.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335561.1
CDS	1..5949
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348588.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348588.1

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348589.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335563.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355201
Clone ID Related Accession (Same CDS sequence)	NM_001348589.1 , NM_001348589.3
Accession Version	NM_001348589.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG TTTAGGAACC GGGTACCAAC GAGATTATCA CACTAGTCAT 
TCACACTCGC ACCATCCCAC CTCACAACAA CAACAACATC AGCCCATGTA CAGCACGTCA 
CCACTAATAT CACCGAGATC GAGTCATAGC TACTACACGC CGAGGAGCTC GCAATACTAT 
GAAATACCAT CGCCGAGTCC AGATATTTAT CCATCGTATC GTGGATCGGC ATCACCTCGC 
AGGTACCCCA CCAGTACCGT CGTAGTGGCT CCAGATCGAG AAGGTTCGAG CGCTCGCGTC 
ATTCAAGCTC AGCCTGGCTC GATACCACTA TCTGACTCGG AAACAGAAGA TGATCCTCGA 
TGGGCAATTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335563.1
CDS	1..5955
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348589.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348589.1

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG TTTAGGAACC GGGTACCAAC GAGATTATCA CACTAGTCAT 
TCACACTCGC ACCATCCCAC CTCACAACAA CAACAACATC AGCCCATGTA CAGCACGTCA 
CCACTAATAT CACCGAGATC GAGTCATAGC TACTACACGC CGAGGAGCTC GCAATACTAT 
GAAATACCAT CGCCGAGTCC AGATATTTAT CCATCGTATC GTGGATCGGC ATCACCTCGC 
AGGTACCCCA CCAGTACCGT CGTAGTGGCT CCAGATCGAG AAGGTTCGAG CGCTCGCGTC 
ATTCAAGCTC AGCCTGGCTC GATACCACTA TCTGACTCGG AAACAGAAGA TGATCCTCGA 
TGGGCAATTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348589.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335563.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355201
Clone ID Related Accession (Same CDS sequence)	NM_001348589.1 , NM_001348589.3
Accession Version	NM_001348589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348589.2. COMPLETENESS: incomplete on the 5' end.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG TTTAGGAACC GGGTACCAAC GAGATTATCA CACTAGTCAT 
TCACACTCGC ACCATCCCAC CTCACAACAA CAACAACATC AGCCCATGTA CAGCACGTCA 
CCACTAATAT CACCGAGATC GAGTCATAGC TACTACACGC CGAGGAGCTC GCAATACTAT 
GAAATACCAT CGCCGAGTCC AGATATTTAT CCATCGTATC GTGGATCGGC ATCACCTCGC 
AGGTACCCCA CCAGTACCGT CGTAGTGGCT CCAGATCGAG AAGGTTCGAG CGCTCGCGTC 
ATTCAAGCTC AGCCTGGCTC GATACCACTA TCTGACTCGG AAACAGAAGA TGATCCTCGA 
TGGGCAATTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335563.1
CDS	1..5955
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348589.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348589.3

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGACC 
GAACATTTGT TGGCTCAAGA TAATCGATCT CCATCGTCAC CTAGATACCG AAGCATGGCC 
CGCGCGTCAC CGCCGAGTCC TGCTGAACGA TACGGTCATC CACCCAGATA TCGCACCGAA 
TCGCCTCCAT CTTCGAGATC GGAGTACCAG ATGTCAATTA GAGACCCGAT AATTAGGCGT 
AATCGCTATA ATACAATGGA ACACTCGCGA TCTTCCCACG ATCCACAATA TCATCAGGAT 
CAACAACAAC AACAACAACC ACATCACCAG CAACACTCAC AACATTTGCA ACATTCACAC 
CACAAAACGT ACCAAAATCA TAATCAATAT TCAAGAAGTC CAATCTATTC CGATGATTCG 
AGTGTTGCTG AAAGTTATCG AAGAGAGCGA GAATTCCGAA GATATCAAGA CTCCACTCCA 
CAAGATGTCA GCGAGGACGA CGACCCGATG CCTACGGCGG TCCGAGCGCG CCGATTACCA 
CTGATTTCCA CAATGCCCAC GCACTACGAG TCGGCCTACC AGCCATCATC ATATAACCAG 
CATCTCAATG ATTCATATGG TTTAGGAACC GGGTACCAAC GAGATTATCA CACTAGTCAT 
TCACACTCGC ACCATCCCAC CTCACAACAA CAACAACATC AGCCCATGTA CAGCACGTCA 
CCACTAATAT CACCGAGATC GAGTCATAGC TACTACACGC CGAGGAGCTC GCAATACTAT 
GAAATACCAT CGCCGAGTCC AGATATTTAT CCATCGTATC GTGGATCGGC ATCACCTCGC 
AGGTACCCCA CCAGTACCGT CGTAGTGGCT CCAGATCGAG AAGGTTCGAG CGCTCGCGTC 
ATTCAAGCTC AGCCTGGCTC GATACCACTA TCTGACTCGG AAACAGAAGA TGATCCTCGA 
TGGGCAATTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348596.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335516.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355209
Clone ID Related Accession (Same CDS sequence)	NM_001348596.1 , NM_001348596.3
Accession Version	NM_001348596.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGGTCGGTGG ACAAGGATTG 
TTTGGCGGAG GCTTACGATC GTTGGTCGCT GCAGCGAAAG CTGCCGAATC GCAACATTCT 
TCCCACACTC CACAACCGCC AGAGGAAACC ACTCCGATAA TTCCACAACA TGCACAGCAG 
TTCTCGGCAG CACCGACGAT GAGCGCACAA GGATCCTTGC AACAAATGCA GGGAACGAGT 
AGTGGCGGTG GTCAACGTCC CTATTCACTC TTCAATAGCT TCGTCGACAC TATCAAATCT 
GGAAAACAGG ATGGAGACGT CACAGATGTG CAATATCAAA GTGTTGATCA GCAGCACGAA 
AAGATGAACT CGACTGGCAG GCGTTTGTCG GATATGTTCT CGAAAATTCG ACGAGGCACA 
TCAGCCGACC ACAATCCGCA TCAGAACTAT TCGCACCGCT TTGGTTACAG TCGCAATAGA 
GGTTCTCGAT ATGATGAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335516.1
CDS	1..5736
Translation	MEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348596.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348596.1

ORF Insert Sequence:

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGGTCGGTGG ACAAGGATTG 
TTTGGCGGAG GCTTACGATC GTTGGTCGCT GCAGCGAAAG CTGCCGAATC GCAACATTCT 
TCCCACACTC CACAACCGCC AGAGGAAACC ACTCCGATAA TTCCACAACA TGCACAGCAG 
TTCTCGGCAG CACCGACGAT GAGCGCACAA GGATCCTTGC AACAAATGCA GGGAACGAGT 
AGTGGCGGTG GTCAACGTCC CTATTCACTC TTCAATAGCT TCGTCGACAC TATCAAATCT 
GGAAAACAGG ATGGAGACGT CACAGATGTG CAATATCAAA GTGTTGATCA GCAGCACGAA 
AAGATGAACT CGACTGGCAG GCGTTTGTCG GATATGTTCT CGAAAATTCG ACGAGGCACA 
TCAGCCGACC ACAATCCGCA TCAGAACTAT TCGCACCGCT TTGGTTACAG TCGCAATAGA 
GGTTCTCGAT ATGATGAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348596.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335516.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355209
Clone ID Related Accession (Same CDS sequence)	NM_001348596.1 , NM_001348596.3
Accession Version	NM_001348596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348596.2. COMPLETENESS: incomplete on the 5' end.

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGGTCGGTGG ACAAGGATTG 
TTTGGCGGAG GCTTACGATC GTTGGTCGCT GCAGCGAAAG CTGCCGAATC GCAACATTCT 
TCCCACACTC CACAACCGCC AGAGGAAACC ACTCCGATAA TTCCACAACA TGCACAGCAG 
TTCTCGGCAG CACCGACGAT GAGCGCACAA GGATCCTTGC AACAAATGCA GGGAACGAGT 
AGTGGCGGTG GTCAACGTCC CTATTCACTC TTCAATAGCT TCGTCGACAC TATCAAATCT 
GGAAAACAGG ATGGAGACGT CACAGATGTG CAATATCAAA GTGTTGATCA GCAGCACGAA 
AAGATGAACT CGACTGGCAG GCGTTTGTCG GATATGTTCT CGAAAATTCG ACGAGGCACA 
TCAGCCGACC ACAATCCGCA TCAGAACTAT TCGCACCGCT TTGGTTACAG TCGCAATAGA 
GGTTCTCGAT ATGATGAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335516.1
CDS	1..5736
Translation	MEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348596.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348596.3

ORF Insert Sequence:

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGGTCGGTGG ACAAGGATTG 
TTTGGCGGAG GCTTACGATC GTTGGTCGCT GCAGCGAAAG CTGCCGAATC GCAACATTCT 
TCCCACACTC CACAACCGCC AGAGGAAACC ACTCCGATAA TTCCACAACA TGCACAGCAG 
TTCTCGGCAG CACCGACGAT GAGCGCACAA GGATCCTTGC AACAAATGCA GGGAACGAGT 
AGTGGCGGTG GTCAACGTCC CTATTCACTC TTCAATAGCT TCGTCGACAC TATCAAATCT 
GGAAAACAGG ATGGAGACGT CACAGATGTG CAATATCAAA GTGTTGATCA GCAGCACGAA 
AAGATGAACT CGACTGGCAG GCGTTTGTCG GATATGTTCT CGAAAATTCG ACGAGGCACA 
TCAGCCGACC ACAATCCGCA TCAGAACTAT TCGCACCGCT TTGGTTACAG TCGCAATAGA 
GGTTCTCGAT ATGATGAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348597.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335515.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355211
Clone ID Related Accession (Same CDS sequence)	NM_001348597.1 , NM_001348597.2
Accession Version	NM_001348597.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGAGGCAGTC AAACGACAGC 
GTGCCGCCGA ACTTCTCCGA AAACGACGAA ATGCGCTGTT CCACTGGTTT TCGAAAGATT 
CCAACGAGTC ACAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA TCGTTGGTCG 
CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG CCAGAGGAAA 
CCACTCCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335515.1
CDS	1..4572
Translation	MEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTERQSNDSVPPNFSENDEMRCSTGFRKIPTSHRSVDKDCLAEAYDRWSLQRKLPNRNILPTLHNRQRKPLR

Target ORF information:

RefSeq Version	NM_001348597.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348597.1

ORF Insert Sequence:

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGAGGCAGTC AAACGACAGC 
GTGCCGCCGA ACTTCTCCGA AAACGACGAA ATGCGCTGTT CCACTGGTTT TCGAAAGATT 
CCAACGAGTC ACAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA TCGTTGGTCG 
CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG CCAGAGGAAA 
CCACTCCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348597.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335515.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355211
Clone ID Related Accession (Same CDS sequence)	NM_001348597.1 , NM_001348597.2
Accession Version	NM_001348597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001348597.1. COMPLETENESS: incomplete on the 5' end.

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGAGGCAGTC AAACGACAGC 
GTGCCGCCGA ACTTCTCCGA AAACGACGAA ATGCGCTGTT CCACTGGTTT TCGAAAGATT 
CCAACGAGTC ACAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA TCGTTGGTCG 
CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG CCAGAGGAAA 
CCACTCCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335515.1
CDS	1..4572
Translation	MEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTERQSNDSVPPNFSENDEMRCSTGFRKIPTSHRSVDKDCLAEAYDRWSLQRKLPNRNILPTLHNRQRKPLR

Target ORF information:

RefSeq Version	NM_001348597.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348597.2

ORF Insert Sequence:

ATGGAGCAAC ATCTGCCGAA AAACGACAAA AAGGCACTGT CGGAATGGCT GGAACGAACC 
GAACCATATT TTATGGGAAT TTTTTGTTTA GAATGTGTTT TGAAAGTCAT AGCGTTTGGT 
TTTGCATTAC ATAAAGGATC ATATCTTCGA TCAGGATGGA ATATCATGGA TTTTATTGTA 
GTAGTTTCCG GTGTCGTCAC CATGTTACCA TTCTCTCCGG CAACACAGAC CGCCAACCAA 
CCGGTGGATT CTGTCGATCT TCGAACATTG CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA 
CTGGTTTCTG GAATTCCAAG TTTGCAAGTT GTCCTCAAGT CGATCTTGTG TGCTATGGCA 
CCATTGTTGC AAATTGGATT ACTTGTGTTG TTTGCCATTA TCATCTTCGC CATCATCGGA 
CTGGAATTTT ATTCGGGTGC ATTTCACAGC GCTTGCTACA ACGAACGCGG TGAAATTGAA 
AATGTTTCCG AGAGGCCAAT GCCGTGCACC AACAAAACGT CTCCAATGGG TGTTTATAAC 
TGTGATGTAA AAGGAACGAC TTGTTTGCAA AAATGGATAG GTCCTAATTA TGGAATTACT 
TCTTTCGACA ATATCGGATT TGCTATGATA ACTGTGTTTC AGTGCATTAC TATGGAAGGG 
TGGACCACTG TAATGTACTA CACAAACGAT TCACTTGGAA GCACATACAA CTGGGCTTAC 
TTTATTCCGC TCATTGTTCT AGGTTCTTTT TTCATGCTCA ATCTCGTTCT CGGAGTATTG 
TCTGGAGAGT TCGCCAAGGA AAGAGAACGT GTTGAAAATC GTCGGGAATT CTTGAAGCTT 
AGAAGGCAAC AACAAATTGA ACGAGAGCTT AACGGATATC TAGAATGGAT TTTAACAGCC 
GAAGAGGTGA TTCTAAAAGA AGATCGAACA ACTGAAGAAG AAAAAGCAGC GATCATGGAA 
GCCAGACGGC GAGCCGCTAA TAAAAAGTTA AAACAAGCCT CAAAACAGCA GTCTACAGAA 
ACTGAAGAGG ACTTTGAGGA GGACGAAGAC GAAATGGAAG AGGAATACGT CGACGAAGCA 
GAACGTAAGA AACGAGGATG TTGCCATTCA GTTGGAAAAT TCATCAAACA ACTGAGAATT 
CAAATCCGTA TCATGGTGAA AACCCAAATC TTCTACTGGT CTGTCATCAC ATTGGTCTTT 
CTAAACACTT GCTGCGTTGC CAGTGAACAC TATGGCCAAC CACAATGGTT CACGGACTTT 
CTGAAATACG CCGAATTCGT GTTCCTCGGA ATTTTCGTAG TTGAAATGCT TTTGAAACTT 
TTCGCAATGG GATCAAGAAC ATATTTTGCA TCTAAATTCA ACCGTTTCGA CTGTGTGGTC 
ATCGTTGGAT CAGCTGCTGA AGTAATTTGG GCTGAAGTGT ATGGAGGATC ATTCGGTATT 
TCGGTGATGA GAGCGCTGAG ACTTCTTCGA ATTTTCAAGC TCACATCATA CTGGGTGTCA 
CTTCGTAATT TAGTCAGATC ACTCATGAAC TCCATGAGGA GTATTATTTC ACTTCTCTTC 
CTACTTTTCT TGTTCATTCT CATTTTCGCC CTTCTTGGAA TGCAGTTGTT CGGAGGAAGA 
TTCAACTTTC CAACAATGCA CCCTTACACA CACTTTGACA CTTTTCCAGT TGCTCTCATT 
ACAGTATTCC AAATCCTTAC TGGAGAAGAT TGGAACGAAG TCATGTATCT CGCGATTGAA 
TCACAAGGAG GTATCTACAG TGGTGGATGG CCATACTCTA TCTACTTTAT TGTTTTAGTA 
CTTTTCGGTA ACTACACTTT GTTGAATGTA TTCTTGGCTA TCGCCGTCGA CAACTTAGCC 
AACGCTCAGG AACTCACTGC AGCTGAAGAA GCAGACGAGA AAGCAAATGA AATAGAGGAA 
GAATCTGAAG AACTTGACGA ACAGTACCAA GAAGGTGATC ATTGCACCAT TGATATGGAG 
GGAAAAACAG CTGGAGACAT GTGTGCGGTT GCAAGAGCTA TGGATGATTT GGACGAAGAA 
TGCGAAGAAG AAGAGTCACC ATTTGGAGGT CCAAAACCCA TGGTTCCCTA CAGTTCTATG 
TTCTTTCTCT CGCCAACTAA TCCATTCCGA GTTCTGATTC ACAGTATCGT GTGCACCAAA 
TACTTTGAAA TGATGGTCAT GACCGTTATC TGTCTCTCAT CGGTTTCTCT AGCCGCTGAA 
GATCCAGTCG ATGAAGAAAA TCCGAGAAAC AAAGTCCTAC AGTATATGGA TTACTGTTTT 
ACTGGAGTTT TTGCATGTGA AATGCTTCTA AAGTTGATTG ATCAAGGTAT TCTTCTACAT 
CCCGGATCAT ATTGCCGCGA TTTCTGGAAT ATTCTTGATG GAATTGTTGT AACATGCGCA 
CTTTTCGCAT TTGGTTTTGC AGGTACCGAA GGAAGTGCTG GTAAAAATTT GAACACAATC 
AAATCGCTGA GGGTACTTCG AGTGCTTCGT CCACTCAAAA CAATCAAAAG AATTCCAAAG 
CTCAAGGCTG TATTCGATTG TGTTGTTAAT TCATTGAAGA ATGTATTCAA CATTCTTATT 
GTTTATTTTC TATTTCAATT CATTTTTGCT GTAATCGCAG TGCAGCTTTT CAACGGGAAA 
TTTTTTTTCT GCACTGACAA GAACCGAAAG TTCGCGAACA CTTGCCACGG GCAGTTCTTT 
GTCTACGATA ATCAGAATGA TCCACCACGG GTTGAACAAC GTGAATGGCG ATTGAGACCG 
TTCAACTACG ACAACACAAT CAACGCAATG CTCACATTAT TCGTTGTAAC GACAGGAGAA 
GGATGGCCTG GCATTCGGCA GAACAGTATG GACACAACGT TCGAAGATCA GGGTCCAAGT 
CCATTTTTTC GGGTTGAAGT GGCTCTATTC TATGTGATGT TTTTCATCGT CTTTCCATTT 
TTCTTCGTCA ACATTTTCGT CGCGTTGATT ATCATCACGT TTCAAGAACA AGGAGAAGCA 
GAGTTAAGCG AAGGAGACTT GGACAAGAAC CAAAAACAAT GTATCGATTT TGCACTGAAT 
GCTCGGCCAA GATCACTATT CATGCCGGAA GACAAGAACT CTACAAAGTA TAGAATATGG 
AGGCTGGTTA CATCACCGCC GTTTGAATAC TTTATCATGA CAATGATTTG CTGCAACACG 
CTCATTCTGA TGATGAAGTA CTACAATAAT CCATTGTTTT ATGAAGAAAT TTTGAGGCTT 
TTCAATACCG CATTAACGGC CGTGTTTACA GTGGAGAGTA TATTGAAGAT TTTGGCATTC 
GGTGTTAGAA ATTACTTTCG TGATGGTTGG AATCGTTTCG ATTTCGTCAC CGTCGTCGGT 
TCTATCACTG ATGCGCTGGT CACAGAGTTC GGAGGTCATT TTGTGTCGCT TGGATTTCTT 
CGACTGTTCC GAGCAGCTCG TCTCATTCGT CTCCTGCAGC AAGGATATAC CATCCGAATC 
CTACTCTGGA CGTTCGTTCA AAGTTTCAAA GCTCTACCGT ATGTGTGTCT TTTGATTGGA 
ATGCTCTTCT TCATTTACGC AATTGTTGGA ATGCAAGTAT TCGGGAATAT CTGGTTGAAT 
GCTGCCACCG AAATCAACAG ACACAACAAT TTCCAGTCTT TCTTCAACGC AGTTATTTTA 
CTCTTCCGAT GCGCGACTGG AGAAGGCTGG CAGGATATTA TGATGGCTGC AGTTCAAGGG 
AAAGATTGCG CACGAGCCGG CTCTGCTGAA ATAAACTTTG AAAAAGGCCA AACTTGCGGT 
AGCAACGTTT CATATGCCTA CTTCACAAGT TTCGTTTTCC TCTCTTCTTT CTTGATGCTC 
AATTTATTCG TTGCCGTCAT CATGGATAAT TTTGACTACC TCACAAGAGA TTCTTCCATT 
CTGGGTCCCC ATCATTTAGA CGAGTTTATC AGAGTCTGGG CAGATTACGA TCCAGCAGCA 
ACGGGCCGAA TTCACTATTC CGAAATGTAT GAGATGTTAA GAATTATGGC ACCTCCTGTC 
GGTTTTGGAA AGAAATGTCC ATACCGATTG GCTTATAAAC ATCTAATCCG AATGAACATG 
CCGGTAGCAG AGGACGGAAC TGTGCACTTT ACAACTACCC TGTTCGCACT GATTCGAGAA 
AGTTTGAGCA TAAAAATGAG ACCGGTGGAA GAGATGGATG AAGCTGATGA GGAATTAAGA 
TTGACATTAA AGAAAATCTG GCCACTGAAA GCAAAGAAAA ACATGGTTGA CTTGGTTGTT 
CCACCGAATC ACGAACTTTG CTTCCAAAAG CTCACAGTGG GAAAAATCTA CGCAGGACTT 
TTGATTCTCG AAAACTATCG AGCTCGAAAA AGCGGGACGG AGAGGCAGTC AAACGACAGC 
GTGCCGCCGA ACTTCTCCGA AAACGACGAA ATGCGCTGTT CCACTGGTTT TCGAAAGATT 
CCAACGAGTC ACAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA TCGTTGGTCG 
CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG CCAGAGGAAA 
CCACTCCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368497.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355494.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa698996
Clone ID Related Accession (Same CDS sequence)	NM_001368497.1 , NM_001368497.3
Accession Version	NM_001368497.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGCTGGGAA TAGACCAGAT TGCAAGACTA GCTGCAGAAG AGGCCCGAAG AGAGCAAAAA 
GCAGAGTCTG GCACATTCGT CAGGAAGACA ACACTTTCAT CCAATGCACC TGTCAAAGAA 
AAGGGTCCGT CGTCCCTATT CATCTTCGCC GAGGACAACA TCATTCGTCG AAACGCGAAG 
GCCATCATAG AATGGGGACC GTTCGAATAC TTCATCTTAT TGACAATTAT AGGCAACTGT 
GTGGTACTAT CCATGGAGCA ACATCTGCCG AAAAACGACA AAAAGGCACT GTCGGAATGG 
CTGGAACGAA CCGAACCATA TTTTATGGGA ATTTTTTGTT TAGAATGTGT TTTGAAAGTC 
ATAGCGTTTG GTTTTGCATT ACATAAAGGA TCATATCTTC GATCAGGATG GAATATCATG 
GATTTTATTG TAGTAGTTTC CGGTGTCGTC ACCATGTTAC CATTCTCTCC GGCAACACAG 
ACCGCCAACC AACCGGTGGA TTCTGTCGAT CTTCGAACAT TGCGAGCTGT TCGAGTGCTT 
CGACCGCTGA AACTGGTTTC TGGAATTCCA AGTTTGCAAG TTGTCCTCAA GTCGATCTTG 
TGTGCTATGG CACCATTGTT GCAAATTGGA TTACTTGTGT TGTTTGCCAT TATCATCTTC 
GCCATCATCG GACTGGAATT TTATTCGGGT GCATTTCACA GCGCTTGCTA CAACGAACGC 
GGTGAAATTG AAAATGTTTC CGAGAGGCCA ATGCCGTGCA CCAACAAAAC GTCTCCAATG 
GGTGTTTATA ACTGTGATGT AAAAGGAACG ACTTGTTTGC AAAAATGGAT AGGTCCTAAT 
TATGGAATTA CTTCTTTCGA CAATATCGGA TTTGCTATGA TAACTGTGTT TCAGTGCATT 
ACTATGGAAG GGTGGACCAC TGTAATGTAC TACACAAACG ATTCACTTGG AAGCACATAC 
AACTGGGCTT ACTTTATTCC GCTCATTGTT CTAGGTTCTT TTTTCATGCT CAATCTCGTT 
CTCGGAGTAT TGTCTGGAGA GTTCGCCAAG GAAAGAGAAC GTGTTGAAAA TCGTCGGGAA 
TTCTTGAAGC TTAGAAGGCA ACAACAAATT GAACGAGAGC TTAACGGATA TCTAGAATGG 
ATTTTAACAG CCGAAGAGGT GATTCTAAAA GAAGATCGAA CAACTGAAGA AGAAAAAGCA 
GCGATCATGG AAGCCAGACG GCGAGCCGCT AATAAAAAGT TAAAACAAGC CTCAAAACAG 
CAGTCTACAG AAACTGAAGA GGACTTTGAG GAGGACGAAG ACGAAATGGA AGAGGAATAC 
GTCGACGAAG GTGGAACAGT AGAAGACGAA TTCGCAGAAC GTAAGAAACG AGGATGTTGC 
CATTCAGTTG GAAAATTCAT CAAACAACTG AGAATTCAAA TCCGTATCAT GGTGAAAACC 
CAAATCTTCT ACTGGTCTGT CATCACATTG GTCTTTCTAA ACACTTGCTG CGTTGCCAGT 
GAACACTATG GCCAACCACA ATGGTTCACG GACTTTCTGA AATACGCCGA ATTCGTGTTC 
CTCGGAATTT TCGTAGTTGA AATGCTTTTG AAACTTTTCG CAATGGGATC AAGAACATAT 
TTTGCATCTA AATTCAACCG TTTCGACTGT GTGGTCATCG TTGGATCAGC TGCTGAAGTA 
ATTTGGGCTG AAGTGTATGG AGGATCATTC GGTATTTCGG TGATGAGAGC GCTGAGACTT 
CTTCGAATTT TCAAGCTCAC ATCATACTGG GTGTCACTTC GTAATTTAGT CAGATCACTC 
ATGAACTCCA TGAGGAGTAT TATTTCACTT CTCTTCCTAC TTTTCTTGTT CATTCTCATT 
TTCGCCCTTC TTGGAATGCA GTTGTTCGGA GGAAGATTCA ACTTTCCAAC AATGCACCCT 
TACACACACT TTGACACTTT TCCAGTTGCT CTCATTACAG TATTCCAAAT CCTTACTGGA 
GAAGATTGGA ACGAAGTCAT GTATCTCGCG ATTGAATCAC AAGGAGGTAT CTACAGTGGT 
GGATGGCCAT ACTCTATCTA CTTTATTGTT TTAGTACTTT TCGGTAACTA CACTTTGTTG 
AATGTATTCT TGGCTATCGC CGTCGACAAC TTAGCCAACG CTCAGGAACT CACTGCAGCT 
GAAGAAGCAG ACGAGAAAGC AAATGAAATA GAGGAAGAAT CTGAAGAACT TGACGAACAG 
TACCAAGAAG GTGATCATTG CACCATTGAT ATGGAGGGAA AAACAGCTGG AGACATGTGT 
GCGGTTGCAA GAGCTATGGA TGATTTGGAC GAAGAATGCG AAGAAGAAGA GTCACCATTT 
GGAGGTCCAA AACCCATGGT TCCCTACAGT TCTATGTTCT TTCTCTCGCC AACTAATCCA 
TTCCGAGTTC TGATTCACAG TATCGTGTGC ACCAAATACT TTGAAATGAT GGTCATGACC 
GTTATCTGTC TCTCATCGGT TTCTCTAGCC GCTGAAGATC CAGTCGATGA AGAAAATCCG 
AGAAACAAAG TCCTACAGTA TATGGATTAC TGTTTTACTG GAGTTTTTGC ATGTGAAATG 
CTTCTAAAGT TGATTGATCA AGGTATTCTT CTACATCCCG GATCATATTG CCGCGATTTC 
TGGAATATTC TTGATGGAAT TGTTGTAACA TGCGCACTTT TCGCATTTGG TTTTGCAGGT 
ACCGAAGGAA GTGCTGGTAA AAATTTGAAC ACAATCAAAT CGCTGAGGGT ACTTCGAGTG 
CTTCGTCCAC TCAAAACAAT CAAAAGAATT CCAAAGCTCA AGGCTGTATT CGATTGTGTT 
GTTAATTCAT TGAAGAATGT ATTCAACATT CTTATTGTTT ATTTTCTATT TCAATTCATT 
TTTGCTGTAA TCGCAGTGCA GCTTTTCAAC GGGAAATTTT TTTTCTGCAC TGACAAGAAC 
CGAAAGTTCG CGAACACTTG CCACGGGCAG TTCTTTGTCT ACGATAATCA GAATGATCCA 
CCACGGGTTG AACAACGTGA ATGGCGATTG AGACCGTTCA ACTACGACAA CACAATCAAC 
GCAATGCTCA CATTATTCGT TGTAACGACA GGAGAAGGAT GGCCTGGCAT TCGGCAGAAC 
AGTATGGACA CAACGTTCGA AGATCAGGGT CCAAGTCCAT TTTTTCGGGT TGAAGTGGCT 
CTATTCTATG TGATGTTTTT CATCGTCTTT CCATTTTTCT TCGTCAACAT TTTCGTCGCG 
TTGATTATCA TCACGTTTCA AGAACAAGGA GAAGCAGAGT TAAGCGAAGG AGACTTGGAC 
AAGAACCAAA AACAATGTAT CGATTTTGCA CTGAATGCTC GGCCAAGATC ACTATTCATG 
CCGGAAGACA AGAACTCTAC AAAGTATAGA ATATGGAGGC TGGTTACATC ACCGCCGTTT 
GAATACTTTA TCATGACAAT GATTTGCTGC AACACGCTCA TTCTGATGAT GAAGTACTAC 
AATAATCCAT TGTTTTATGA AGAAATTTTG AGGCTTTTCA ATACCGCATT AACGGCCGTG 
TTTACAGTGG AGAGTATATT GAAGATTTTG GCATTCGGTG TTAGAAATTA CTTTCGTGAT 
GGTTGGAATC GTTTCGATTT CGTCACCGTC GTCGGTTCTA TCACTGATGC GCTGGTCACA 
GAGTTCGGAG GTCATTTTGT GTCGCTTGGA TTTCTTCGAC TGTTCCGAGC AGCTCGTCTC 
ATTCGTCTCC TGCAGCAAGG ATATACCATC CGAATCCTAC TCTGGACGTT CGTTCAAAGT 
TTCAAAGCTC TACCGTATGT GTGTCTTTTG ATTGGAATGC TCTTCTTCAT TTACGCAATT 
GTTGGAATGC AAGTATTCGG GAATATCTGG TTGAATGCTG CCACCGAAAT CAACAGACAC 
AACAATTTCC AGTCTTTCTT CAACGCAGTT ATTTTACTCT TCCGATGCGC GACTGGAGAA 
GGCTGGCAGG ATATTATGAT GGCTGCAGTT CAAGGGAAAG ATTGCGCACG AGCCGGCTCT 
GCTGAAATAA ACTTTGAAAA AGGCCAAACT TGCGGTAGCA ACGTTTCATA TGCCTACTTC 
ACAAGTTTCG TTTTCCTCTC TTCTTTCTTG ATGCTCAATT TATTCGTTGC CGTCATCATG 
GATAATTTTG ACTACCTCAC AAGAGATTCT TCCATTCTGG GTCCCCATCA TTTAGACGAG 
TTTATCAGAG TCTGGGCAGA TTACGATCCA GCAGCAACGG GCCGAATTCA CTATTCCGAA 
ATGTATGAGA TGTTAAGAAT TATGGCACCT CCTGTCGGTT TTGGAAAGAA ATGTCCATAC 
CGATTGGCTT ATAAACATCT AATCCGAATG AACATGCCGG TAGCAGAGGA CGGAACTGTG 
CACTTTACAA CTACCCTGTT CGCACTGATT CGAGAAAGTT TGAGCATAAA AATGAGACCG 
GTGGAAGAGA TGGATGAAGC TGATGAGGAA TTAAGATTGA CATTAAAGAA AATCTGGCCA 
CTGAAAGCAA AGAAAAACAT GGTTGACTTG GTTGTTCCAC CGAATCACGA ACTTTGCTTC 
CAAAAGCTCA CAGTGGGAAA AATCTACGCA GGACTTTTGA TTCTCGAAAA CTATCGAGCT 
CGAAAAAGCG GGACGGAGGT CGGTGGACAA GGATTGTTTG GCGGAGGCTT ACGATCGTTG 
GTCGCTGCAG CGAAAGCTGC CGAATCGCAA CATTCTTCCC ACACTCCACA ACCGCCAGAG 
GAAACCACTC CGATAATTCC ACAACATGCA CAGCAGTTCT CGGCAGCACC GACGATGAGC 
GCACAAGGAT CCTTGCAACA AATGCAGGGA ACGAGTAGTG GCGGTGGTCA ACGTCCCTAT 
TCACTCTTCA ATAGCTTCGT CGACACTATC AAATCTGGAA AACAGGATGG AGACGTCACA 
GATGTGCAAT ATCAAAGTGT TGATCAGCAG CACGAAAAGA TGAACTCGAC TGGCAGGCGT 
TTGTCGGATA TGTTCTCGAA AATTCGACGA GGCACATCAG CCGACCACAA TCCGCATCAG 
ACCGAACATT TGTTGGCTCA AGATAATCGA TCTCCATCGT CACCTAGATA CCGAAGCATG 
GCCCGCGCGT CACCGCCGAG TCCTGCTGAA CGATACGGTC ATCCACCCAG ATATCGCACC 
GAATCGCCTC CATCTTCGAG ATCGGAGTAC CAGATGTCAA TTAGAGACCC GATAATTAGG 
CGTAATCGCT ATAATACAAT GGAACACTCG CGATCTTCCC ACGATCCACA ATATCATCAG 
GATCAACAAC AACAACAACA ACCACATCAC CAGCAACACT CACAACATTT GCAACATTCA 
CACCACAAAA CGTACCAAAA TCATAATCAA TATTCAAGAA GTCCAATCTA TTCCGATGAT 
TCGAGTGTTG CTGAAAGTTA TCGAAGAGAG CGAGAATTCC GAAGATATCA AGACTCCACT 
CCACAAGATG TCAGCGAGGA CGACGACCCG ATGCCTACGG CGGTCCGAGC GCGCCGATTA 
CCACTGATTT CCACAATGCC CACGCACTAC GAGTCGGCCT ACCAGCCATC ATCATATAAC 
CAGCATCTCA ATGATTCATA TGGTTTAGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355494.1
CDS	1..5958
Translation	MLGIDQIARLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368497.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368497.1

ORF Insert Sequence:

ATGCTGGGAA TAGACCAGAT TGCAAGACTA GCTGCAGAAG AGGCCCGAAG AGAGCAAAAA 
GCAGAGTCTG GCACATTCGT CAGGAAGACA ACACTTTCAT CCAATGCACC TGTCAAAGAA 
AAGGGTCCGT CGTCCCTATT CATCTTCGCC GAGGACAACA TCATTCGTCG AAACGCGAAG 
GCCATCATAG AATGGGGACC GTTCGAATAC TTCATCTTAT TGACAATTAT AGGCAACTGT 
GTGGTACTAT CCATGGAGCA ACATCTGCCG AAAAACGACA AAAAGGCACT GTCGGAATGG 
CTGGAACGAA CCGAACCATA TTTTATGGGA ATTTTTTGTT TAGAATGTGT TTTGAAAGTC 
ATAGCGTTTG GTTTTGCATT ACATAAAGGA TCATATCTTC GATCAGGATG GAATATCATG 
GATTTTATTG TAGTAGTTTC CGGTGTCGTC ACCATGTTAC CATTCTCTCC GGCAACACAG 
ACCGCCAACC AACCGGTGGA TTCTGTCGAT CTTCGAACAT TGCGAGCTGT TCGAGTGCTT 
CGACCGCTGA AACTGGTTTC TGGAATTCCA AGTTTGCAAG TTGTCCTCAA GTCGATCTTG 
TGTGCTATGG CACCATTGTT GCAAATTGGA TTACTTGTGT TGTTTGCCAT TATCATCTTC 
GCCATCATCG GACTGGAATT TTATTCGGGT GCATTTCACA GCGCTTGCTA CAACGAACGC 
GGTGAAATTG AAAATGTTTC CGAGAGGCCA ATGCCGTGCA CCAACAAAAC GTCTCCAATG 
GGTGTTTATA ACTGTGATGT AAAAGGAACG ACTTGTTTGC AAAAATGGAT AGGTCCTAAT 
TATGGAATTA CTTCTTTCGA CAATATCGGA TTTGCTATGA TAACTGTGTT TCAGTGCATT 
ACTATGGAAG GGTGGACCAC TGTAATGTAC TACACAAACG ATTCACTTGG AAGCACATAC 
AACTGGGCTT ACTTTATTCC GCTCATTGTT CTAGGTTCTT TTTTCATGCT CAATCTCGTT 
CTCGGAGTAT TGTCTGGAGA GTTCGCCAAG GAAAGAGAAC GTGTTGAAAA TCGTCGGGAA 
TTCTTGAAGC TTAGAAGGCA ACAACAAATT GAACGAGAGC TTAACGGATA TCTAGAATGG 
ATTTTAACAG CCGAAGAGGT GATTCTAAAA GAAGATCGAA CAACTGAAGA AGAAAAAGCA 
GCGATCATGG AAGCCAGACG GCGAGCCGCT AATAAAAAGT TAAAACAAGC CTCAAAACAG 
CAGTCTACAG AAACTGAAGA GGACTTTGAG GAGGACGAAG ACGAAATGGA AGAGGAATAC 
GTCGACGAAG GTGGAACAGT AGAAGACGAA TTCGCAGAAC GTAAGAAACG AGGATGTTGC 
CATTCAGTTG GAAAATTCAT CAAACAACTG AGAATTCAAA TCCGTATCAT GGTGAAAACC 
CAAATCTTCT ACTGGTCTGT CATCACATTG GTCTTTCTAA ACACTTGCTG CGTTGCCAGT 
GAACACTATG GCCAACCACA ATGGTTCACG GACTTTCTGA AATACGCCGA ATTCGTGTTC 
CTCGGAATTT TCGTAGTTGA AATGCTTTTG AAACTTTTCG CAATGGGATC AAGAACATAT 
TTTGCATCTA AATTCAACCG TTTCGACTGT GTGGTCATCG TTGGATCAGC TGCTGAAGTA 
ATTTGGGCTG AAGTGTATGG AGGATCATTC GGTATTTCGG TGATGAGAGC GCTGAGACTT 
CTTCGAATTT TCAAGCTCAC ATCATACTGG GTGTCACTTC GTAATTTAGT CAGATCACTC 
ATGAACTCCA TGAGGAGTAT TATTTCACTT CTCTTCCTAC TTTTCTTGTT CATTCTCATT 
TTCGCCCTTC TTGGAATGCA GTTGTTCGGA GGAAGATTCA ACTTTCCAAC AATGCACCCT 
TACACACACT TTGACACTTT TCCAGTTGCT CTCATTACAG TATTCCAAAT CCTTACTGGA 
GAAGATTGGA ACGAAGTCAT GTATCTCGCG ATTGAATCAC AAGGAGGTAT CTACAGTGGT 
GGATGGCCAT ACTCTATCTA CTTTATTGTT TTAGTACTTT TCGGTAACTA CACTTTGTTG 
AATGTATTCT TGGCTATCGC CGTCGACAAC TTAGCCAACG CTCAGGAACT CACTGCAGCT 
GAAGAAGCAG ACGAGAAAGC AAATGAAATA GAGGAAGAAT CTGAAGAACT TGACGAACAG 
TACCAAGAAG GTGATCATTG CACCATTGAT ATGGAGGGAA AAACAGCTGG AGACATGTGT 
GCGGTTGCAA GAGCTATGGA TGATTTGGAC GAAGAATGCG AAGAAGAAGA GTCACCATTT 
GGAGGTCCAA AACCCATGGT TCCCTACAGT TCTATGTTCT TTCTCTCGCC AACTAATCCA 
TTCCGAGTTC TGATTCACAG TATCGTGTGC ACCAAATACT TTGAAATGAT GGTCATGACC 
GTTATCTGTC TCTCATCGGT TTCTCTAGCC GCTGAAGATC CAGTCGATGA AGAAAATCCG 
AGAAACAAAG TCCTACAGTA TATGGATTAC TGTTTTACTG GAGTTTTTGC ATGTGAAATG 
CTTCTAAAGT TGATTGATCA AGGTATTCTT CTACATCCCG GATCATATTG CCGCGATTTC 
TGGAATATTC TTGATGGAAT TGTTGTAACA TGCGCACTTT TCGCATTTGG TTTTGCAGGT 
ACCGAAGGAA GTGCTGGTAA AAATTTGAAC ACAATCAAAT CGCTGAGGGT ACTTCGAGTG 
CTTCGTCCAC TCAAAACAAT CAAAAGAATT CCAAAGCTCA AGGCTGTATT CGATTGTGTT 
GTTAATTCAT TGAAGAATGT ATTCAACATT CTTATTGTTT ATTTTCTATT TCAATTCATT 
TTTGCTGTAA TCGCAGTGCA GCTTTTCAAC GGGAAATTTT TTTTCTGCAC TGACAAGAAC 
CGAAAGTTCG CGAACACTTG CCACGGGCAG TTCTTTGTCT ACGATAATCA GAATGATCCA 
CCACGGGTTG AACAACGTGA ATGGCGATTG AGACCGTTCA ACTACGACAA CACAATCAAC 
GCAATGCTCA CATTATTCGT TGTAACGACA GGAGAAGGAT GGCCTGGCAT TCGGCAGAAC 
AGTATGGACA CAACGTTCGA AGATCAGGGT CCAAGTCCAT TTTTTCGGGT TGAAGTGGCT 
CTATTCTATG TGATGTTTTT CATCGTCTTT CCATTTTTCT TCGTCAACAT TTTCGTCGCG 
TTGATTATCA TCACGTTTCA AGAACAAGGA GAAGCAGAGT TAAGCGAAGG AGACTTGGAC 
AAGAACCAAA AACAATGTAT CGATTTTGCA CTGAATGCTC GGCCAAGATC ACTATTCATG 
CCGGAAGACA AGAACTCTAC AAAGTATAGA ATATGGAGGC TGGTTACATC ACCGCCGTTT 
GAATACTTTA TCATGACAAT GATTTGCTGC AACACGCTCA TTCTGATGAT GAAGTACTAC 
AATAATCCAT TGTTTTATGA AGAAATTTTG AGGCTTTTCA ATACCGCATT AACGGCCGTG 
TTTACAGTGG AGAGTATATT GAAGATTTTG GCATTCGGTG TTAGAAATTA CTTTCGTGAT 
GGTTGGAATC GTTTCGATTT CGTCACCGTC GTCGGTTCTA TCACTGATGC GCTGGTCACA 
GAGTTCGGAG GTCATTTTGT GTCGCTTGGA TTTCTTCGAC TGTTCCGAGC AGCTCGTCTC 
ATTCGTCTCC TGCAGCAAGG ATATACCATC CGAATCCTAC TCTGGACGTT CGTTCAAAGT 
TTCAAAGCTC TACCGTATGT GTGTCTTTTG ATTGGAATGC TCTTCTTCAT TTACGCAATT 
GTTGGAATGC AAGTATTCGG GAATATCTGG TTGAATGCTG CCACCGAAAT CAACAGACAC 
AACAATTTCC AGTCTTTCTT CAACGCAGTT ATTTTACTCT TCCGATGCGC GACTGGAGAA 
GGCTGGCAGG ATATTATGAT GGCTGCAGTT CAAGGGAAAG ATTGCGCACG AGCCGGCTCT 
GCTGAAATAA ACTTTGAAAA AGGCCAAACT TGCGGTAGCA ACGTTTCATA TGCCTACTTC 
ACAAGTTTCG TTTTCCTCTC TTCTTTCTTG ATGCTCAATT TATTCGTTGC CGTCATCATG 
GATAATTTTG ACTACCTCAC AAGAGATTCT TCCATTCTGG GTCCCCATCA TTTAGACGAG 
TTTATCAGAG TCTGGGCAGA TTACGATCCA GCAGCAACGG GCCGAATTCA CTATTCCGAA 
ATGTATGAGA TGTTAAGAAT TATGGCACCT CCTGTCGGTT TTGGAAAGAA ATGTCCATAC 
CGATTGGCTT ATAAACATCT AATCCGAATG AACATGCCGG TAGCAGAGGA CGGAACTGTG 
CACTTTACAA CTACCCTGTT CGCACTGATT CGAGAAAGTT TGAGCATAAA AATGAGACCG 
GTGGAAGAGA TGGATGAAGC TGATGAGGAA TTAAGATTGA CATTAAAGAA AATCTGGCCA 
CTGAAAGCAA AGAAAAACAT GGTTGACTTG GTTGTTCCAC CGAATCACGA ACTTTGCTTC 
CAAAAGCTCA CAGTGGGAAA AATCTACGCA GGACTTTTGA TTCTCGAAAA CTATCGAGCT 
CGAAAAAGCG GGACGGAGGT CGGTGGACAA GGATTGTTTG GCGGAGGCTT ACGATCGTTG 
GTCGCTGCAG CGAAAGCTGC CGAATCGCAA CATTCTTCCC ACACTCCACA ACCGCCAGAG 
GAAACCACTC CGATAATTCC ACAACATGCA CAGCAGTTCT CGGCAGCACC GACGATGAGC 
GCACAAGGAT CCTTGCAACA AATGCAGGGA ACGAGTAGTG GCGGTGGTCA ACGTCCCTAT 
TCACTCTTCA ATAGCTTCGT CGACACTATC AAATCTGGAA AACAGGATGG AGACGTCACA 
GATGTGCAAT ATCAAAGTGT TGATCAGCAG CACGAAAAGA TGAACTCGAC TGGCAGGCGT 
TTGTCGGATA TGTTCTCGAA AATTCGACGA GGCACATCAG CCGACCACAA TCCGCATCAG 
ACCGAACATT TGTTGGCTCA AGATAATCGA TCTCCATCGT CACCTAGATA CCGAAGCATG 
GCCCGCGCGT CACCGCCGAG TCCTGCTGAA CGATACGGTC ATCCACCCAG ATATCGCACC 
GAATCGCCTC CATCTTCGAG ATCGGAGTAC CAGATGTCAA TTAGAGACCC GATAATTAGG 
CGTAATCGCT ATAATACAAT GGAACACTCG CGATCTTCCC ACGATCCACA ATATCATCAG 
GATCAACAAC AACAACAACA ACCACATCAC CAGCAACACT CACAACATTT GCAACATTCA 
CACCACAAAA CGTACCAAAA TCATAATCAA TATTCAAGAA GTCCAATCTA TTCCGATGAT 
TCGAGTGTTG CTGAAAGTTA TCGAAGAGAG CGAGAATTCC GAAGATATCA AGACTCCACT 
CCACAAGATG TCAGCGAGGA CGACGACCCG ATGCCTACGG CGGTCCGAGC GCGCCGATTA 
CCACTGATTT CCACAATGCC CACGCACTAC GAGTCGGCCT ACCAGCCATC ATCATATAAC 
CAGCATCTCA ATGATTCATA TGGTTTAGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368497.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355494.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), mRNA.

Price & Availability↑

CloneID	OCa698996
Clone ID Related Accession (Same CDS sequence)	NM_001368497.1 , NM_001368497.3
Accession Version	NM_001368497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368497.2.

ATGCTGGGAA TAGACCAGAT TGCAAGACTA GCTGCAGAAG AGGCCCGAAG AGAGCAAAAA 
GCAGAGTCTG GCACATTCGT CAGGAAGACA ACACTTTCAT CCAATGCACC TGTCAAAGAA 
AAGGGTCCGT CGTCCCTATT CATCTTCGCC GAGGACAACA TCATTCGTCG AAACGCGAAG 
GCCATCATAG AATGGGGACC GTTCGAATAC TTCATCTTAT TGACAATTAT AGGCAACTGT 
GTGGTACTAT CCATGGAGCA ACATCTGCCG AAAAACGACA AAAAGGCACT GTCGGAATGG 
CTGGAACGAA CCGAACCATA TTTTATGGGA ATTTTTTGTT TAGAATGTGT TTTGAAAGTC 
ATAGCGTTTG GTTTTGCATT ACATAAAGGA TCATATCTTC GATCAGGATG GAATATCATG 
GATTTTATTG TAGTAGTTTC CGGTGTCGTC ACCATGTTAC CATTCTCTCC GGCAACACAG 
ACCGCCAACC AACCGGTGGA TTCTGTCGAT CTTCGAACAT TGCGAGCTGT TCGAGTGCTT 
CGACCGCTGA AACTGGTTTC TGGAATTCCA AGTTTGCAAG TTGTCCTCAA GTCGATCTTG 
TGTGCTATGG CACCATTGTT GCAAATTGGA TTACTTGTGT TGTTTGCCAT TATCATCTTC 
GCCATCATCG GACTGGAATT TTATTCGGGT GCATTTCACA GCGCTTGCTA CAACGAACGC 
GGTGAAATTG AAAATGTTTC CGAGAGGCCA ATGCCGTGCA CCAACAAAAC GTCTCCAATG 
GGTGTTTATA ACTGTGATGT AAAAGGAACG ACTTGTTTGC AAAAATGGAT AGGTCCTAAT 
TATGGAATTA CTTCTTTCGA CAATATCGGA TTTGCTATGA TAACTGTGTT TCAGTGCATT 
ACTATGGAAG GGTGGACCAC TGTAATGTAC TACACAAACG ATTCACTTGG AAGCACATAC 
AACTGGGCTT ACTTTATTCC GCTCATTGTT CTAGGTTCTT TTTTCATGCT CAATCTCGTT 
CTCGGAGTAT TGTCTGGAGA GTTCGCCAAG GAAAGAGAAC GTGTTGAAAA TCGTCGGGAA 
TTCTTGAAGC TTAGAAGGCA ACAACAAATT GAACGAGAGC TTAACGGATA TCTAGAATGG 
ATTTTAACAG CCGAAGAGGT GATTCTAAAA GAAGATCGAA CAACTGAAGA AGAAAAAGCA 
GCGATCATGG AAGCCAGACG GCGAGCCGCT AATAAAAAGT TAAAACAAGC CTCAAAACAG 
CAGTCTACAG AAACTGAAGA GGACTTTGAG GAGGACGAAG ACGAAATGGA AGAGGAATAC 
GTCGACGAAG GTGGAACAGT AGAAGACGAA TTCGCAGAAC GTAAGAAACG AGGATGTTGC 
CATTCAGTTG GAAAATTCAT CAAACAACTG AGAATTCAAA TCCGTATCAT GGTGAAAACC 
CAAATCTTCT ACTGGTCTGT CATCACATTG GTCTTTCTAA ACACTTGCTG CGTTGCCAGT 
GAACACTATG GCCAACCACA ATGGTTCACG GACTTTCTGA AATACGCCGA ATTCGTGTTC 
CTCGGAATTT TCGTAGTTGA AATGCTTTTG AAACTTTTCG CAATGGGATC AAGAACATAT 
TTTGCATCTA AATTCAACCG TTTCGACTGT GTGGTCATCG TTGGATCAGC TGCTGAAGTA 
ATTTGGGCTG AAGTGTATGG AGGATCATTC GGTATTTCGG TGATGAGAGC GCTGAGACTT 
CTTCGAATTT TCAAGCTCAC ATCATACTGG GTGTCACTTC GTAATTTAGT CAGATCACTC 
ATGAACTCCA TGAGGAGTAT TATTTCACTT CTCTTCCTAC TTTTCTTGTT CATTCTCATT 
TTCGCCCTTC TTGGAATGCA GTTGTTCGGA GGAAGATTCA ACTTTCCAAC AATGCACCCT 
TACACACACT TTGACACTTT TCCAGTTGCT CTCATTACAG TATTCCAAAT CCTTACTGGA 
GAAGATTGGA ACGAAGTCAT GTATCTCGCG ATTGAATCAC AAGGAGGTAT CTACAGTGGT 
GGATGGCCAT ACTCTATCTA CTTTATTGTT TTAGTACTTT TCGGTAACTA CACTTTGTTG 
AATGTATTCT TGGCTATCGC CGTCGACAAC TTAGCCAACG CTCAGGAACT CACTGCAGCT 
GAAGAAGCAG ACGAGAAAGC AAATGAAATA GAGGAAGAAT CTGAAGAACT TGACGAACAG 
TACCAAGAAG GTGATCATTG CACCATTGAT ATGGAGGGAA AAACAGCTGG AGACATGTGT 
GCGGTTGCAA GAGCTATGGA TGATTTGGAC GAAGAATGCG AAGAAGAAGA GTCACCATTT 
GGAGGTCCAA AACCCATGGT TCCCTACAGT TCTATGTTCT TTCTCTCGCC AACTAATCCA 
TTCCGAGTTC TGATTCACAG TATCGTGTGC ACCAAATACT TTGAAATGAT GGTCATGACC 
GTTATCTGTC TCTCATCGGT TTCTCTAGCC GCTGAAGATC CAGTCGATGA AGAAAATCCG 
AGAAACAAAG TCCTACAGTA TATGGATTAC TGTTTTACTG GAGTTTTTGC ATGTGAAATG 
CTTCTAAAGT TGATTGATCA AGGTATTCTT CTACATCCCG GATCATATTG CCGCGATTTC 
TGGAATATTC TTGATGGAAT TGTTGTAACA TGCGCACTTT TCGCATTTGG TTTTGCAGGT 
ACCGAAGGAA GTGCTGGTAA AAATTTGAAC ACAATCAAAT CGCTGAGGGT ACTTCGAGTG 
CTTCGTCCAC TCAAAACAAT CAAAAGAATT CCAAAGCTCA AGGCTGTATT CGATTGTGTT 
GTTAATTCAT TGAAGAATGT ATTCAACATT CTTATTGTTT ATTTTCTATT TCAATTCATT 
TTTGCTGTAA TCGCAGTGCA GCTTTTCAAC GGGAAATTTT TTTTCTGCAC TGACAAGAAC 
CGAAAGTTCG CGAACACTTG CCACGGGCAG TTCTTTGTCT ACGATAATCA GAATGATCCA 
CCACGGGTTG AACAACGTGA ATGGCGATTG AGACCGTTCA ACTACGACAA CACAATCAAC 
GCAATGCTCA CATTATTCGT TGTAACGACA GGAGAAGGAT GGCCTGGCAT TCGGCAGAAC 
AGTATGGACA CAACGTTCGA AGATCAGGGT CCAAGTCCAT TTTTTCGGGT TGAAGTGGCT 
CTATTCTATG TGATGTTTTT CATCGTCTTT CCATTTTTCT TCGTCAACAT TTTCGTCGCG 
TTGATTATCA TCACGTTTCA AGAACAAGGA GAAGCAGAGT TAAGCGAAGG AGACTTGGAC 
AAGAACCAAA AACAATGTAT CGATTTTGCA CTGAATGCTC GGCCAAGATC ACTATTCATG 
CCGGAAGACA AGAACTCTAC AAAGTATAGA ATATGGAGGC TGGTTACATC ACCGCCGTTT 
GAATACTTTA TCATGACAAT GATTTGCTGC AACACGCTCA TTCTGATGAT GAAGTACTAC 
AATAATCCAT TGTTTTATGA AGAAATTTTG AGGCTTTTCA ATACCGCATT AACGGCCGTG 
TTTACAGTGG AGAGTATATT GAAGATTTTG GCATTCGGTG TTAGAAATTA CTTTCGTGAT 
GGTTGGAATC GTTTCGATTT CGTCACCGTC GTCGGTTCTA TCACTGATGC GCTGGTCACA 
GAGTTCGGAG GTCATTTTGT GTCGCTTGGA TTTCTTCGAC TGTTCCGAGC AGCTCGTCTC 
ATTCGTCTCC TGCAGCAAGG ATATACCATC CGAATCCTAC TCTGGACGTT CGTTCAAAGT 
TTCAAAGCTC TACCGTATGT GTGTCTTTTG ATTGGAATGC TCTTCTTCAT TTACGCAATT 
GTTGGAATGC AAGTATTCGG GAATATCTGG TTGAATGCTG CCACCGAAAT CAACAGACAC 
AACAATTTCC AGTCTTTCTT CAACGCAGTT ATTTTACTCT TCCGATGCGC GACTGGAGAA 
GGCTGGCAGG ATATTATGAT GGCTGCAGTT CAAGGGAAAG ATTGCGCACG AGCCGGCTCT 
GCTGAAATAA ACTTTGAAAA AGGCCAAACT TGCGGTAGCA ACGTTTCATA TGCCTACTTC 
ACAAGTTTCG TTTTCCTCTC TTCTTTCTTG ATGCTCAATT TATTCGTTGC CGTCATCATG 
GATAATTTTG ACTACCTCAC AAGAGATTCT TCCATTCTGG GTCCCCATCA TTTAGACGAG 
TTTATCAGAG TCTGGGCAGA TTACGATCCA GCAGCAACGG GCCGAATTCA CTATTCCGAA 
ATGTATGAGA TGTTAAGAAT TATGGCACCT CCTGTCGGTT TTGGAAAGAA ATGTCCATAC 
CGATTGGCTT ATAAACATCT AATCCGAATG AACATGCCGG TAGCAGAGGA CGGAACTGTG 
CACTTTACAA CTACCCTGTT CGCACTGATT CGAGAAAGTT TGAGCATAAA AATGAGACCG 
GTGGAAGAGA TGGATGAAGC TGATGAGGAA TTAAGATTGA CATTAAAGAA AATCTGGCCA 
CTGAAAGCAA AGAAAAACAT GGTTGACTTG GTTGTTCCAC CGAATCACGA ACTTTGCTTC 
CAAAAGCTCA CAGTGGGAAA AATCTACGCA GGACTTTTGA TTCTCGAAAA CTATCGAGCT 
CGAAAAAGCG GGACGGAGGT CGGTGGACAA GGATTGTTTG GCGGAGGCTT ACGATCGTTG 
GTCGCTGCAG CGAAAGCTGC CGAATCGCAA CATTCTTCCC ACACTCCACA ACCGCCAGAG 
GAAACCACTC CGATAATTCC ACAACATGCA CAGCAGTTCT CGGCAGCACC GACGATGAGC 
GCACAAGGAT CCTTGCAACA AATGCAGGGA ACGAGTAGTG GCGGTGGTCA ACGTCCCTAT 
TCACTCTTCA ATAGCTTCGT CGACACTATC AAATCTGGAA AACAGGATGG AGACGTCACA 
GATGTGCAAT ATCAAAGTGT TGATCAGCAG CACGAAAAGA TGAACTCGAC TGGCAGGCGT 
TTGTCGGATA TGTTCTCGAA AATTCGACGA GGCACATCAG CCGACCACAA TCCGCATCAG 
ACCGAACATT TGTTGGCTCA AGATAATCGA TCTCCATCGT CACCTAGATA CCGAAGCATG 
GCCCGCGCGT CACCGCCGAG TCCTGCTGAA CGATACGGTC ATCCACCCAG ATATCGCACC 
GAATCGCCTC CATCTTCGAG ATCGGAGTAC CAGATGTCAA TTAGAGACCC GATAATTAGG 
CGTAATCGCT ATAATACAAT GGAACACTCG CGATCTTCCC ACGATCCACA ATATCATCAG 
GATCAACAAC AACAACAACA ACCACATCAC CAGCAACACT CACAACATTT GCAACATTCA 
CACCACAAAA CGTACCAAAA TCATAATCAA TATTCAAGAA GTCCAATCTA TTCCGATGAT 
TCGAGTGTTG CTGAAAGTTA TCGAAGAGAG CGAGAATTCC GAAGATATCA AGACTCCACT 
CCACAAGATG TCAGCGAGGA CGACGACCCG ATGCCTACGG CGGTCCGAGC GCGCCGATTA 
CCACTGATTT CCACAATGCC CACGCACTAC GAGTCGGCCT ACCAGCCATC ATCATATAAC 
CAGCATCTCA ATGATTCATA TGGTTTAGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355494.1
CDS	326..6283
Translation	MLGIDQIARLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368497.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368497.3

ORF Insert Sequence:

ATGCTGGGAA TAGACCAGAT TGCAAGACTA GCTGCAGAAG AGGCCCGAAG AGAGCAAAAA 
GCAGAGTCTG GCACATTCGT CAGGAAGACA ACACTTTCAT CCAATGCACC TGTCAAAGAA 
AAGGGTCCGT CGTCCCTATT CATCTTCGCC GAGGACAACA TCATTCGTCG AAACGCGAAG 
GCCATCATAG AATGGGGACC GTTCGAATAC TTCATCTTAT TGACAATTAT AGGCAACTGT 
GTGGTACTAT CCATGGAGCA ACATCTGCCG AAAAACGACA AAAAGGCACT GTCGGAATGG 
CTGGAACGAA CCGAACCATA TTTTATGGGA ATTTTTTGTT TAGAATGTGT TTTGAAAGTC 
ATAGCGTTTG GTTTTGCATT ACATAAAGGA TCATATCTTC GATCAGGATG GAATATCATG 
GATTTTATTG TAGTAGTTTC CGGTGTCGTC ACCATGTTAC CATTCTCTCC GGCAACACAG 
ACCGCCAACC AACCGGTGGA TTCTGTCGAT CTTCGAACAT TGCGAGCTGT TCGAGTGCTT 
CGACCGCTGA AACTGGTTTC TGGAATTCCA AGTTTGCAAG TTGTCCTCAA GTCGATCTTG 
TGTGCTATGG CACCATTGTT GCAAATTGGA TTACTTGTGT TGTTTGCCAT TATCATCTTC 
GCCATCATCG GACTGGAATT TTATTCGGGT GCATTTCACA GCGCTTGCTA CAACGAACGC 
GGTGAAATTG AAAATGTTTC CGAGAGGCCA ATGCCGTGCA CCAACAAAAC GTCTCCAATG 
GGTGTTTATA ACTGTGATGT AAAAGGAACG ACTTGTTTGC AAAAATGGAT AGGTCCTAAT 
TATGGAATTA CTTCTTTCGA CAATATCGGA TTTGCTATGA TAACTGTGTT TCAGTGCATT 
ACTATGGAAG GGTGGACCAC TGTAATGTAC TACACAAACG ATTCACTTGG AAGCACATAC 
AACTGGGCTT ACTTTATTCC GCTCATTGTT CTAGGTTCTT TTTTCATGCT CAATCTCGTT 
CTCGGAGTAT TGTCTGGAGA GTTCGCCAAG GAAAGAGAAC GTGTTGAAAA TCGTCGGGAA 
TTCTTGAAGC TTAGAAGGCA ACAACAAATT GAACGAGAGC TTAACGGATA TCTAGAATGG 
ATTTTAACAG CCGAAGAGGT GATTCTAAAA GAAGATCGAA CAACTGAAGA AGAAAAAGCA 
GCGATCATGG AAGCCAGACG GCGAGCCGCT AATAAAAAGT TAAAACAAGC CTCAAAACAG 
CAGTCTACAG AAACTGAAGA GGACTTTGAG GAGGACGAAG ACGAAATGGA AGAGGAATAC 
GTCGACGAAG GTGGAACAGT AGAAGACGAA TTCGCAGAAC GTAAGAAACG AGGATGTTGC 
CATTCAGTTG GAAAATTCAT CAAACAACTG AGAATTCAAA TCCGTATCAT GGTGAAAACC 
CAAATCTTCT ACTGGTCTGT CATCACATTG GTCTTTCTAA ACACTTGCTG CGTTGCCAGT 
GAACACTATG GCCAACCACA ATGGTTCACG GACTTTCTGA AATACGCCGA ATTCGTGTTC 
CTCGGAATTT TCGTAGTTGA AATGCTTTTG AAACTTTTCG CAATGGGATC AAGAACATAT 
TTTGCATCTA AATTCAACCG TTTCGACTGT GTGGTCATCG TTGGATCAGC TGCTGAAGTA 
ATTTGGGCTG AAGTGTATGG AGGATCATTC GGTATTTCGG TGATGAGAGC GCTGAGACTT 
CTTCGAATTT TCAAGCTCAC ATCATACTGG GTGTCACTTC GTAATTTAGT CAGATCACTC 
ATGAACTCCA TGAGGAGTAT TATTTCACTT CTCTTCCTAC TTTTCTTGTT CATTCTCATT 
TTCGCCCTTC TTGGAATGCA GTTGTTCGGA GGAAGATTCA ACTTTCCAAC AATGCACCCT 
TACACACACT TTGACACTTT TCCAGTTGCT CTCATTACAG TATTCCAAAT CCTTACTGGA 
GAAGATTGGA ACGAAGTCAT GTATCTCGCG ATTGAATCAC AAGGAGGTAT CTACAGTGGT 
GGATGGCCAT ACTCTATCTA CTTTATTGTT TTAGTACTTT TCGGTAACTA CACTTTGTTG 
AATGTATTCT TGGCTATCGC CGTCGACAAC TTAGCCAACG CTCAGGAACT CACTGCAGCT 
GAAGAAGCAG ACGAGAAAGC AAATGAAATA GAGGAAGAAT CTGAAGAACT TGACGAACAG 
TACCAAGAAG GTGATCATTG CACCATTGAT ATGGAGGGAA AAACAGCTGG AGACATGTGT 
GCGGTTGCAA GAGCTATGGA TGATTTGGAC GAAGAATGCG AAGAAGAAGA GTCACCATTT 
GGAGGTCCAA AACCCATGGT TCCCTACAGT TCTATGTTCT TTCTCTCGCC AACTAATCCA 
TTCCGAGTTC TGATTCACAG TATCGTGTGC ACCAAATACT TTGAAATGAT GGTCATGACC 
GTTATCTGTC TCTCATCGGT TTCTCTAGCC GCTGAAGATC CAGTCGATGA AGAAAATCCG 
AGAAACAAAG TCCTACAGTA TATGGATTAC TGTTTTACTG GAGTTTTTGC ATGTGAAATG 
CTTCTAAAGT TGATTGATCA AGGTATTCTT CTACATCCCG GATCATATTG CCGCGATTTC 
TGGAATATTC TTGATGGAAT TGTTGTAACA TGCGCACTTT TCGCATTTGG TTTTGCAGGT 
ACCGAAGGAA GTGCTGGTAA AAATTTGAAC ACAATCAAAT CGCTGAGGGT ACTTCGAGTG 
CTTCGTCCAC TCAAAACAAT CAAAAGAATT CCAAAGCTCA AGGCTGTATT CGATTGTGTT 
GTTAATTCAT TGAAGAATGT ATTCAACATT CTTATTGTTT ATTTTCTATT TCAATTCATT 
TTTGCTGTAA TCGCAGTGCA GCTTTTCAAC GGGAAATTTT TTTTCTGCAC TGACAAGAAC 
CGAAAGTTCG CGAACACTTG CCACGGGCAG TTCTTTGTCT ACGATAATCA GAATGATCCA 
CCACGGGTTG AACAACGTGA ATGGCGATTG AGACCGTTCA ACTACGACAA CACAATCAAC 
GCAATGCTCA CATTATTCGT TGTAACGACA GGAGAAGGAT GGCCTGGCAT TCGGCAGAAC 
AGTATGGACA CAACGTTCGA AGATCAGGGT CCAAGTCCAT TTTTTCGGGT TGAAGTGGCT 
CTATTCTATG TGATGTTTTT CATCGTCTTT CCATTTTTCT TCGTCAACAT TTTCGTCGCG 
TTGATTATCA TCACGTTTCA AGAACAAGGA GAAGCAGAGT TAAGCGAAGG AGACTTGGAC 
AAGAACCAAA AACAATGTAT CGATTTTGCA CTGAATGCTC GGCCAAGATC ACTATTCATG 
CCGGAAGACA AGAACTCTAC AAAGTATAGA ATATGGAGGC TGGTTACATC ACCGCCGTTT 
GAATACTTTA TCATGACAAT GATTTGCTGC AACACGCTCA TTCTGATGAT GAAGTACTAC 
AATAATCCAT TGTTTTATGA AGAAATTTTG AGGCTTTTCA ATACCGCATT AACGGCCGTG 
TTTACAGTGG AGAGTATATT GAAGATTTTG GCATTCGGTG TTAGAAATTA CTTTCGTGAT 
GGTTGGAATC GTTTCGATTT CGTCACCGTC GTCGGTTCTA TCACTGATGC GCTGGTCACA 
GAGTTCGGAG GTCATTTTGT GTCGCTTGGA TTTCTTCGAC TGTTCCGAGC AGCTCGTCTC 
ATTCGTCTCC TGCAGCAAGG ATATACCATC CGAATCCTAC TCTGGACGTT CGTTCAAAGT 
TTCAAAGCTC TACCGTATGT GTGTCTTTTG ATTGGAATGC TCTTCTTCAT TTACGCAATT 
GTTGGAATGC AAGTATTCGG GAATATCTGG TTGAATGCTG CCACCGAAAT CAACAGACAC 
AACAATTTCC AGTCTTTCTT CAACGCAGTT ATTTTACTCT TCCGATGCGC GACTGGAGAA 
GGCTGGCAGG ATATTATGAT GGCTGCAGTT CAAGGGAAAG ATTGCGCACG AGCCGGCTCT 
GCTGAAATAA ACTTTGAAAA AGGCCAAACT TGCGGTAGCA ACGTTTCATA TGCCTACTTC 
ACAAGTTTCG TTTTCCTCTC TTCTTTCTTG ATGCTCAATT TATTCGTTGC CGTCATCATG 
GATAATTTTG ACTACCTCAC AAGAGATTCT TCCATTCTGG GTCCCCATCA TTTAGACGAG 
TTTATCAGAG TCTGGGCAGA TTACGATCCA GCAGCAACGG GCCGAATTCA CTATTCCGAA 
ATGTATGAGA TGTTAAGAAT TATGGCACCT CCTGTCGGTT TTGGAAAGAA ATGTCCATAC 
CGATTGGCTT ATAAACATCT AATCCGAATG AACATGCCGG TAGCAGAGGA CGGAACTGTG 
CACTTTACAA CTACCCTGTT CGCACTGATT CGAGAAAGTT TGAGCATAAA AATGAGACCG 
GTGGAAGAGA TGGATGAAGC TGATGAGGAA TTAAGATTGA CATTAAAGAA AATCTGGCCA 
CTGAAAGCAA AGAAAAACAT GGTTGACTTG GTTGTTCCAC CGAATCACGA ACTTTGCTTC 
CAAAAGCTCA CAGTGGGAAA AATCTACGCA GGACTTTTGA TTCTCGAAAA CTATCGAGCT 
CGAAAAAGCG GGACGGAGGT CGGTGGACAA GGATTGTTTG GCGGAGGCTT ACGATCGTTG 
GTCGCTGCAG CGAAAGCTGC CGAATCGCAA CATTCTTCCC ACACTCCACA ACCGCCAGAG 
GAAACCACTC CGATAATTCC ACAACATGCA CAGCAGTTCT CGGCAGCACC GACGATGAGC 
GCACAAGGAT CCTTGCAACA AATGCAGGGA ACGAGTAGTG GCGGTGGTCA ACGTCCCTAT 
TCACTCTTCA ATAGCTTCGT CGACACTATC AAATCTGGAA AACAGGATGG AGACGTCACA 
GATGTGCAAT ATCAAAGTGT TGATCAGCAG CACGAAAAGA TGAACTCGAC TGGCAGGCGT 
TTGTCGGATA TGTTCTCGAA AATTCGACGA GGCACATCAG CCGACCACAA TCCGCATCAG 
ACCGAACATT TGTTGGCTCA AGATAATCGA TCTCCATCGT CACCTAGATA CCGAAGCATG 
GCCCGCGCGT CACCGCCGAG TCCTGCTGAA CGATACGGTC ATCCACCCAG ATATCGCACC 
GAATCGCCTC CATCTTCGAG ATCGGAGTAC CAGATGTCAA TTAGAGACCC GATAATTAGG 
CGTAATCGCT ATAATACAAT GGAACACTCG CGATCTTCCC ACGATCCACA ATATCATCAG 
GATCAACAAC AACAACAACA ACCACATCAC CAGCAACACT CACAACATTT GCAACATTCA 
CACCACAAAA CGTACCAAAA TCATAATCAA TATTCAAGAA GTCCAATCTA TTCCGATGAT 
TCGAGTGTTG CTGAAAGTTA TCGAAGAGAG CGAGAATTCC GAAGATATCA AGACTCCACT 
CCACAAGATG TCAGCGAGGA CGACGACCCG ATGCCTACGG CGGTCCGAGC GCGCCGATTA 
CCACTGATTT CCACAATGCC CACGCACTAC GAGTCGGCCT ACCAGCCATC ATCATATAAC 
CAGCATCTCA ATGATTCATA TGGTTTAGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001129704.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001123176.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Price & Availability↑

CloneID	OCa355199
Clone ID Related Accession (Same CDS sequence)	NM_001129704.1
Accession Version	NM_001129704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGTGTATCC GTTTAAAATA CCTAAAATCG TTATTCTTCT TAGAGAGAGA CTCGACTACT 
TTTGATCAGC GTTCTGCTGC CCGTCGGGCG TCAGTTCTCG GTCGATCAGC TGCCGAATCG 
TTATCTGCTC AAGAAGCAGC ATCGAGCAGC GAACGGGGAG AGCACACTAA CAGTCGCTCC 
CCATCGACCA GCTATTCGAG TTGTATCGAC GACGAACGAA AGTTCTCGAG TCCTCATCGT 
AGACGAGTAG TCGATGTCAG CGATCATAAG ACATGTGCAC TGTTGATGAC GAGGATGAAA 
GAGGCAAGTA GACAGCTGCC ATCGCCGAGC CAACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123176.1
CDS	1..6264
Translation	MCIRLKYLKSLFFLERDSTTFDQRSAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001129704.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129704.1

ORF Insert Sequence:

ATGTGTATCC GTTTAAAATA CCTAAAATCG TTATTCTTCT TAGAGAGAGA CTCGACTACT 
TTTGATCAGC GTTCTGCTGC CCGTCGGGCG TCAGTTCTCG GTCGATCAGC TGCCGAATCG 
TTATCTGCTC AAGAAGCAGC ATCGAGCAGC GAACGGGGAG AGCACACTAA CAGTCGCTCC 
CCATCGACCA GCTATTCGAG TTGTATCGAC GACGAACGAA AGTTCTCGAG TCCTCATCGT 
AGACGAGTAG TCGATGTCAG CGATCATAAG ACATGTGCAC TGTTGATGAC GAGGATGAAA 
GAGGCAAGTA GACAGCTGCC ATCGCCGAGC CAACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348590.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335560.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355202
Clone ID Related Accession (Same CDS sequence)	NM_001348590.1 , NM_001348590.3
Accession Version	NM_001348590.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGAACCGG GTACCAACGA GATTATCACA CTAGTCATTC ACACTCGCAC 
CATCCCACCT CACAACAACA ACAACATCAG CCCATGTACA GCACGTCACC ACTAATATCA 
CCGAGATCGA GTCATAGCTA CTACACGCCG AGGAGCTCGC AATACTATGA AATACCATCG 
CCGAGTCCAG ATATTTATCC ATCGTATCGT GGATCGGCAT CACCTCGCAG GTACCCCACC 
AGTACCGTCG TAGTGGCTCC AGATCGAGAA GGTTCGAGCG CTCGCGTCAT TCAAGCTCAG 
CCTGGCTCGA TACCACTATC TGACTCGGAA ACAGAAGATG ATCCTCGATG GGCAATTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335560.1
CDS	1..6003
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348590.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348590.1

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGAACCGG GTACCAACGA GATTATCACA CTAGTCATTC ACACTCGCAC 
CATCCCACCT CACAACAACA ACAACATCAG CCCATGTACA GCACGTCACC ACTAATATCA 
CCGAGATCGA GTCATAGCTA CTACACGCCG AGGAGCTCGC AATACTATGA AATACCATCG 
CCGAGTCCAG ATATTTATCC ATCGTATCGT GGATCGGCAT CACCTCGCAG GTACCCCACC 
AGTACCGTCG TAGTGGCTCC AGATCGAGAA GGTTCGAGCG CTCGCGTCAT TCAAGCTCAG 
CCTGGCTCGA TACCACTATC TGACTCGGAA ACAGAAGATG ATCCTCGATG GGCAATTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348590.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335560.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355202
Clone ID Related Accession (Same CDS sequence)	NM_001348590.1 , NM_001348590.3
Accession Version	NM_001348590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348590.2. COMPLETENESS: incomplete on the 5' end.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGAACCGG GTACCAACGA GATTATCACA CTAGTCATTC ACACTCGCAC 
CATCCCACCT CACAACAACA ACAACATCAG CCCATGTACA GCACGTCACC ACTAATATCA 
CCGAGATCGA GTCATAGCTA CTACACGCCG AGGAGCTCGC AATACTATGA AATACCATCG 
CCGAGTCCAG ATATTTATCC ATCGTATCGT GGATCGGCAT CACCTCGCAG GTACCCCACC 
AGTACCGTCG TAGTGGCTCC AGATCGAGAA GGTTCGAGCG CTCGCGTCAT TCAAGCTCAG 
CCTGGCTCGA TACCACTATC TGACTCGGAA ACAGAAGATG ATCCTCGATG GGCAATTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335560.1
CDS	1..6003
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348590.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348590.3

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGAACCGG GTACCAACGA GATTATCACA CTAGTCATTC ACACTCGCAC 
CATCCCACCT CACAACAACA ACAACATCAG CCCATGTACA GCACGTCACC ACTAATATCA 
CCGAGATCGA GTCATAGCTA CTACACGCCG AGGAGCTCGC AATACTATGA AATACCATCG 
CCGAGTCCAG ATATTTATCC ATCGTATCGT GGATCGGCAT CACCTCGCAG GTACCCCACC 
AGTACCGTCG TAGTGGCTCC AGATCGAGAA GGTTCGAGCG CTCGCGTCAT TCAAGCTCAG 
CCTGGCTCGA TACCACTATC TGACTCGGAA ACAGAAGATG ATCCTCGATG GGCAATTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348591.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335562.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355203
Clone ID Related Accession (Same CDS sequence)	NM_001348591.3 , NM_001348591.1
Accession Version	NM_001348591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348591.2. COMPLETENESS: incomplete on the 5' end.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGTTTAGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335562.1
CDS	1..6009
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348591.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348591.3

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGTTTAGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348591.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335562.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355203
Clone ID Related Accession (Same CDS sequence)	NM_001348591.3 , NM_001348591.1
Accession Version	NM_001348591.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGTTTAGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335562.1
CDS	1..6009
Translation	MTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348591.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348591.1

ORF Insert Sequence:

ATGACGAGGA TGAAAGAGGC AAGTAGACAG CTGCCATCGC CGAGCCAACT AGCTGCAGAA 
GAGGCCCGAA GAGAGCAAAA AGCAGAGTCT GGCACATTCG TCAGGAAGAC AACACTTTCA 
TCCAATGCAC CTGTCAAAGA AAAGGGTCCG TCGTCCCTAT TCATCTTCGC CGAGGACAAC 
ATCATTCGTC GAAACGCGAA GGCCATCATA GAATGGGGAC CGTTCGAATA CTTCATCTTA 
TTGACAATTA TAGGCAACTG TGTGGTACTA TCCATGGAGC AACATCTGCC GAAAAACGAC 
AAAAAGGCAC TGTCGGAATG GCTGGAACGA ACCGAACCAT ATTTTATGGG AATTTTTTGT 
TTAGAATGTG TTTTGAAAGT CATAGCGTTT GGTTTTGCAT TACATAAAGG ATCATATCTT 
CGATCAGGAT GGAATATCAT GGATTTTATT GTAGTAGTTT CCGGTGTCGT CACCATGTTA 
CCATTCTCTC CGGCAACACA GACCGCCAAC CAACCGGTGG ATTCTGTCGA TCTTCGAACA 
TTGCGAGCTG TTCGAGTGCT TCGACCGCTG AAACTGGTTT CTGGAATTCC AAGTTTGCAA 
GTTGTCCTCA AGTCGATCTT GTGTGCTATG GCACCATTGT TGCAAATTGG ATTACTTGTG 
TTGTTTGCCA TTATCATCTT CGCCATCATC GGACTGGAAT TTTATTCGGG TGCATTTCAC 
AGCGCTTGCT ACAACGAACG CGGTGAAATT GAAAATGTTT CCGAGAGGCC AATGCCGTGC 
ACCAACAAAA CGTCTCCAAT GGGTGTTTAT AACTGTGATG TAAAAGGAAC GACTTGTTTG 
CAAAAATGGA TAGGTCCTAA TTATGGAATT ACTTCTTTCG ACAATATCGG ATTTGCTATG 
ATAACTGTGT TTCAGTGCAT TACTATGGAA GGGTGGACCA CTGTAATGTA CTACACAAAC 
GATTCACTTG GAAGCACATA CAACTGGGCT TACTTTATTC CGCTCATTGT TCTAGGTTCT 
TTTTTCATGC TCAATCTCGT TCTCGGAGTA TTGTCTGGAG AGTTCGCCAA GGAAAGAGAA 
CGTGTTGAAA ATCGTCGGGA ATTCTTGAAG CTTAGAAGGC AACAACAAAT TGAACGAGAG 
CTTAACGGAT ATCTAGAATG GATTTTAACA GCCGAAGAGG TGATTCTAAA AGAAGATCGA 
ACAACTGAAG AAGAAAAAGC AGCGATCATG GAAGCCAGAC GGCGAGCCGC TAATAAAAAG 
TTAAAACAAG CCTCAAAACA GCAGTCTACA GAAACTGAAG AGGACTTTGA GGAGGACGAA 
GACGAAATGG AAGAGGAATA CGTCGACGAA GCAGAACGTA AGAAACGAGG ATGTTGCCAT 
TCAGTTGGAA AATTCATCAA ACAACTGAGA ATTCAAATCC GTATCATGGT GAAAACCCAA 
ATCTTCTACT GGTCTGTCAT CACATTGGTC TTTCTAAACA CTTGCTGCGT TGCCAGTGAA 
CACTATGGCC AACCACAATG GTTCACGGAC TTTCTGAAAT ACGCCGAATT CGTGTTCCTC 
GGAATTTTCG TAGTTGAAAT GCTTTTGAAA CTTTTCGCAA TGGGATCAAG AACATATTTT 
GCATCTAAAT TCAACCGTTT CGACTGTGTG GTCATCGTTG GATCAGCTGC TGAAGTAATT 
TGGGCTGAAG TGTATGGAGG ATCATTCGGT ATTTCGGTGA TGAGAGCGCT GAGACTTCTT 
CGAATTTTCA AGCTCACATC ATACTGGGTG TCACTTCGTA ATTTAGTCAG ATCACTCATG 
AACTCCATGA GGAGTATTAT TTCACTTCTC TTCCTACTTT TCTTGTTCAT TCTCATTTTC 
GCCCTTCTTG GAATGCAGTT GTTCGGAGGA AGATTCAACT TTCCAACAAT GCACCCTTAC 
ACACACTTTG ACACTTTTCC AGTTGCTCTC ATTACAGTAT TCCAAATCCT TACTGGAGAA 
GATTGGAACG AAGTCATGTA TCTCGCGATT GAATCACAAG GAGGTATCTA CAGTGGTGGA 
TGGCCATACT CTATCTACTT TATTGTTTTA GTACTTTTCG GTAACTACAC TTTGTTGAAT 
GTATTCTTGG CTATCGCCGT CGACAACTTA GCCAACGCTC AGGAACTCAC TGCAGCTGAA 
GAAGCAGACG AGAAAGCAAA TGAAATAGAG GAAGAATCTG AAGAACTTGA CGAACAGTAC 
CAAGAAGGTG ATCATTGCAC CATTGATATG GAGGGAAAAA CAGCTGGAGA CATGTGTGCG 
GTTGCAAGAG CTATGGATGA TTTGGACGAA GAATGCGAAG AAGAAGAGTC ACCATTTGGA 
GGTCCAAAAC CCATGGTTCC CTACAGTTCT ATGTTCTTTC TCTCGCCAAC TAATCCATTC 
CGAGTTCTGA TTCACAGTAT CGTGTGCACC AAATACTTTG AAATGATGGT CATGACCGTT 
ATCTGTCTCT CATCGGTTTC TCTAGCCGCT GAAGATCCAG TCGATGAAGA AAATCCGAGA 
AACAAAGTCC TACAGTATAT GGATTACTGT TTTACTGGAG TTTTTGCATG TGAAATGCTT 
CTAAAGTTGA TTGATCAAGG TATTCTTCTA CATCCCGGAT CATATTGCCG CGATTTCTGG 
AATATTCTTG ATGGAATTGT TGTAACATGC GCACTTTTCG CATTTGGTTT TGCAGGTACC 
GAAGGAAGTG CTGGTAAAAA TTTGAACACA ATCAAATCGC TGAGGGTACT TCGAGTGCTT 
CGTCCACTCA AAACAATCAA AAGAATTCCA AAGCTCAAGG CTGTATTCGA TTGTGTTGTT 
AATTCATTGA AGAATGTATT CAACATTCTT ATTGTTTATT TTCTATTTCA ATTCATTTTT 
GCTGTAATCG CAGTGCAGCT TTTCAACGGG AAATTTTTTT TCTGCACTGA CAAGAACCGA 
AAGTTCGCGA ACACTTGCCA CGGGCAGTTC TTTGTCTACG ATAATCAGAA TGATCCACCA 
CGGGTTGAAC AACGTGAATG GCGATTGAGA CCGTTCAACT ACGACAACAC AATCAACGCA 
ATGCTCACAT TATTCGTTGT AACGACAGGA GAAGGATGGC CTGGCATTCG GCAGAACAGT 
ATGGACACAA CGTTCGAAGA TCAGGGTCCA AGTCCATTTT TTCGGGTTGA AGTGGCTCTA 
TTCTATGTGA TGTTTTTCAT CGTCTTTCCA TTTTTCTTCG TCAACATTTT CGTCGCGTTG 
ATTATCATCA CGTTTCAAGA ACAAGGAGAA GCAGAGTTAA GCGAAGGAGA CTTGGACAAG 
AACCAAAAAC AATGTATCGA TTTTGCACTG AATGCTCGGC CAAGATCACT ATTCATGCCG 
GAAGACAAGA ACTCTACAAA GTATAGAATA TGGAGGCTGG TTACATCACC GCCGTTTGAA 
TACTTTATCA TGACAATGAT TTGCTGCAAC ACGCTCATTC TGATGATGAA GTACTACAAT 
AATCCATTGT TTTATGAAGA AATTTTGAGG CTTTTCAATA CCGCATTAAC GGCCGTGTTT 
ACAGTGGAGA GTATATTGAA GATTTTGGCA TTCGGTGTTA GAAATTACTT TCGTGATGGT 
TGGAATCGTT TCGATTTCGT CACCGTCGTC GGTTCTATCA CTGATGCGCT GGTCACAGAG 
TTCGGAGGTC ATTTTGTGTC GCTTGGATTT CTTCGACTGT TCCGAGCAGC TCGTCTCATT 
CGTCTCCTGC AGCAAGGATA TACCATCCGA ATCCTACTCT GGACGTTCGT TCAAAGTTTC 
AAAGCTCTAC CGTATGTGTG TCTTTTGATT GGAATGCTCT TCTTCATTTA CGCAATTGTT 
GGAATGCAAG TATTCGGGAA TATCTGGTTG AATGCTGCCA CCGAAATCAA CAGACACAAC 
AATTTCCAGT CTTTCTTCAA CGCAGTTATT TTACTCTTCC GATGCGCGAC TGGAGAAGGC 
TGGCAGGATA TTATGATGGC TGCAGTTCAA GGGAAAGATT GCGCACGAGC CGGCTCTGCT 
GAAATAAACT TTGAAAAAGG CCAAACTTGC GGTAGCAACG TTTCATATGC CTACTTCACA 
AGTTTCGTTT TCCTCTCTTC TTTCTTGATG CTCAATTTAT TCGTTGCCGT CATCATGGAT 
AATTTTGACT ACCTCACAAG AGATTCTTCC ATTCTGGGTC CCCATCATTT AGACGAGTTT 
ATCAGAGTCT GGGCAGATTA CGATCCAGCA GCAACGGGCC GAATTCACTA TTCCGAAATG 
TATGAGATGT TAAGAATTAT GGCACCTCCT GTCGGTTTTG GAAAGAAATG TCCATACCGA 
TTGGCTTATA AACATCTAAT CCGAATGAAC ATGCCGGTAG CAGAGGACGG AACTGTGCAC 
TTTACAACTA CCCTGTTCGC ACTGATTCGA GAAAGTTTGA GCATAAAAAT GAGACCGGTG 
GAAGAGATGG ATGAAGCTGA TGAGGAATTA AGATTGACAT TAAAGAAAAT CTGGCCACTG 
AAAGCAAAGA AAAACATGGT TGACTTGGTT GTTCCACCGA ATCACGAACT TTGCTTCCAA 
AAGCTCACAG TGGGAAAAAT CTACGCAGGA CTTTTGATTC TCGAAAACTA TCGAGCTCGA 
AAAAGCGGGA CGGAGGTCGG TGGACAAGGA TTGTTTGGCG GAGGCTTACG ATCGTTGGTC 
GCTGCAGCGA AAGCTGCCGA ATCGCAACAT TCTTCCCACA CTCCACAACC GCCAGAGGAA 
ACCACTCCGA TAATTCCACA ACATGCACAG CAGTTCTCGG CAGCACCGAC GATGAGCGCA 
CAAGGATCCT TGCAACAAAT GCAGGGAACG AGTAGTGGCG GTGGTCAACG TCCCTATTCA 
CTCTTCAATA GCTTCGTCGA CACTATCAAA TCTGGAAAAC AGGATGGAGA CGTCACAGAT 
GTGCAATATC AAAGTGTTGA TCAGCAGCAC GAAAAGATGA ACTCGACTGG CAGGCGTTTG 
TCGGATATGT TCTCGAAAAT TCGACGAGGC ACATCAGCCG ACCACAATCC GCATCAGAAC 
TATTCGCACC GCTTTGGTTA CAGTCGCAAT AGAGGTTCTC GATATGATGA GACCGAACAT 
TTGTTGGCTC AAGATAATCG ATCTCCATCG TCACCTAGAT ACCGAAGCAT GGCCCGCGCG 
TCACCGCCGA GTCCTGCTGA ACGATACGGT CATCCACCCA GATATCGCAC CGAATCGCCT 
CCATCTTCGA GATCGGAGTA CCAGATGTCA ATTAGAGACC CGATAATTAG GCGTAATCGC 
TATAATACAA TGGAACACTC GCGATCTTCC CACGATCCAC AATATCATCA GGATCAACAA 
CAACAACAAC AACCACATCA CCAGCAACAC TCACAACATT TGCAACATTC ACACCACAAA 
ACGTACCAAA ATCATAATCA ATATTCAAGA AGTCCAATCT ATTCCGATGA TTCGAGTGTT 
GCTGAAAGTT ATCGAAGAGA GCGAGAATTC CGAAGATATC AAGACTCCAC TCCACAAGAT 
GTCAGCGAGG ACGACGACCC GATGCCTACG GCGGTCCGAG CGCGCCGATT ACCACTGATT 
TCCACAATGC CCACGCACTA CGAGTCGGCC TACCAGCCAT CATCATATAA CCAGCATCTC 
AATGATTCAT ATGGTTTAGG AACCGGGTAC CAACGAGATT ATCACACTAG TCATTCACAC 
TCGCACCATC CCACCTCACA ACAACAACAA CATCAGCCCA TGTACAGCAC GTCACCACTA 
ATATCACCGA GATCGAGTCA TAGCTACTAC ACGCCGAGGA GCTCGCAATA CTATGAAATA 
CCATCGCCGA GTCCAGATAT TTATCCATCG TATCGTGGAT CGGCATCACC TCGCAGGTAC 
CCCACCAGTA CCGTCGTAGT GGCTCCAGAT CGAGAAGGTT CGAGCGCTCG CGTCATTCAA 
GCTCAGCCTG GCTCGATACC ACTATCTGAC TCGGAAACAG AAGATGATCC TCGATGGGCA 
ATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348592.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335571.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355204
Clone ID Related Accession (Same CDS sequence)	NM_001348592.1 , NM_001348592.3
Accession Version	NM_001348592.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335571.1
CDS	1..6144
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348592.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348592.1

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348592.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335571.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355204
Clone ID Related Accession (Same CDS sequence)	NM_001348592.1 , NM_001348592.3
Accession Version	NM_001348592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348592.2. COMPLETENESS: incomplete on the 5' end.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335571.1
CDS	1..6144
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348592.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348592.3

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348593.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335573.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355205
Clone ID Related Accession (Same CDS sequence)	NM_001348593.1 , NM_001348593.3
Accession Version	NM_001348593.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGTTTAG GAACCGGGTA CCAACGAGAT 
TATCACACTA GTCATTCACA CTCGCACCAT CCCACCTCAC AACAACAACA ACATCAGCCC 
ATGTACAGCA CGTCACCACT AATATCACCG AGATCGAGTC ATAGCTACTA CACGCCGAGG 
AGCTCGCAAT ACTATGAAAT ACCATCGCCG AGTCCAGATA TTTATCCATC GTATCGTGGA 
TCGGCATCAC CTCGCAGGTA CCCCACCAGT ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT 
TCGAGCGCTC GCGTCATTCA AGCTCAGCCT GGCTCGATAC CACTATCTGA CTCGGAAACA 
GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335573.1
CDS	1..6150
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348593.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348593.1

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGTTTAG GAACCGGGTA CCAACGAGAT 
TATCACACTA GTCATTCACA CTCGCACCAT CCCACCTCAC AACAACAACA ACATCAGCCC 
ATGTACAGCA CGTCACCACT AATATCACCG AGATCGAGTC ATAGCTACTA CACGCCGAGG 
AGCTCGCAAT ACTATGAAAT ACCATCGCCG AGTCCAGATA TTTATCCATC GTATCGTGGA 
TCGGCATCAC CTCGCAGGTA CCCCACCAGT ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT 
TCGAGCGCTC GCGTCATTCA AGCTCAGCCT GGCTCGATAC CACTATCTGA CTCGGAAACA 
GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348593.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335573.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355205
Clone ID Related Accession (Same CDS sequence)	NM_001348593.1 , NM_001348593.3
Accession Version	NM_001348593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348593.2. COMPLETENESS: incomplete on the 5' end.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGTTTAG GAACCGGGTA CCAACGAGAT 
TATCACACTA GTCATTCACA CTCGCACCAT CCCACCTCAC AACAACAACA ACATCAGCCC 
ATGTACAGCA CGTCACCACT AATATCACCG AGATCGAGTC ATAGCTACTA CACGCCGAGG 
AGCTCGCAAT ACTATGAAAT ACCATCGCCG AGTCCAGATA TTTATCCATC GTATCGTGGA 
TCGGCATCAC CTCGCAGGTA CCCCACCAGT ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT 
TCGAGCGCTC GCGTCATTCA AGCTCAGCCT GGCTCGATAC CACTATCTGA CTCGGAAACA 
GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335573.1
CDS	1..6150
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348593.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348593.3

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGACCGAACA TTTGTTGGCT CAAGATAATC GATCTCCATC GTCACCTAGA 
TACCGAAGCA TGGCCCGCGC GTCACCGCCG AGTCCTGCTG AACGATACGG TCATCCACCC 
AGATATCGCA CCGAATCGCC TCCATCTTCG AGATCGGAGT ACCAGATGTC AATTAGAGAC 
CCGATAATTA GGCGTAATCG CTATAATACA ATGGAACACT CGCGATCTTC CCACGATCCA 
CAATATCATC AGGATCAACA ACAACAACAA CAACCACATC ACCAGCAACA CTCACAACAT 
TTGCAACATT CACACCACAA AACGTACCAA AATCATAATC AATATTCAAG AAGTCCAATC 
TATTCCGATG ATTCGAGTGT TGCTGAAAGT TATCGAAGAG AGCGAGAATT CCGAAGATAT 
CAAGACTCCA CTCCACAAGA TGTCAGCGAG GACGACGACC CGATGCCTAC GGCGGTCCGA 
GCGCGCCGAT TACCACTGAT TTCCACAATG CCCACGCACT ACGAGTCGGC CTACCAGCCA 
TCATCATATA ACCAGCATCT CAATGATTCA TATGGTTTAG GAACCGGGTA CCAACGAGAT 
TATCACACTA GTCATTCACA CTCGCACCAT CCCACCTCAC AACAACAACA ACATCAGCCC 
ATGTACAGCA CGTCACCACT AATATCACCG AGATCGAGTC ATAGCTACTA CACGCCGAGG 
AGCTCGCAAT ACTATGAAAT ACCATCGCCG AGTCCAGATA TTTATCCATC GTATCGTGGA 
TCGGCATCAC CTCGCAGGTA CCCCACCAGT ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT 
TCGAGCGCTC GCGTCATTCA AGCTCAGCCT GGCTCGATAC CACTATCTGA CTCGGAAACA 
GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348594.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335570.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355206
Clone ID Related Accession (Same CDS sequence)	NM_001348594.1 , NM_001348594.3
Accession Version	NM_001348594.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335570.1
CDS	1..6198
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348594.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348594.1

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348594.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335570.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355206
Clone ID Related Accession (Same CDS sequence)	NM_001348594.1 , NM_001348594.3
Accession Version	NM_001348594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348594.2. COMPLETENESS: incomplete on the 5' end.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335570.1
CDS	1..6198
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348594.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348594.3

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGA ACCGGGTACC AACGAGATTA TCACACTAGT 
CATTCACACT CGCACCATCC CACCTCACAA CAACAACAAC ATCAGCCCAT GTACAGCACG 
TCACCACTAA TATCACCGAG ATCGAGTCAT AGCTACTACA CGCCGAGGAG CTCGCAATAC 
TATGAAATAC CATCGCCGAG TCCAGATATT TATCCATCGT ATCGTGGATC GGCATCACCT 
CGCAGGTACC CCACCAGTAC CGTCGTAGTG GCTCCAGATC GAGAAGGTTC GAGCGCTCGC 
GTCATTCAAG CTCAGCCTGG CTCGATACCA CTATCTGACT CGGAAACAGA AGATGATCCT 
CGATGGGCAA TTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348595.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335572.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355207
Clone ID Related Accession (Same CDS sequence)	NM_001348595.1 , NM_001348595.3
Accession Version	NM_001348595.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335572.1
CDS	1..6204
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348595.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348595.1

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001348595.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001335572.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa355207
Clone ID Related Accession (Same CDS sequence)	NM_001348595.1 , NM_001348595.3
Accession Version	NM_001348595.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001348595.2. COMPLETENESS: incomplete on the 5' end.

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335572.1
CDS	1..6204
Translation	MGNDGDGASGSKPNGSVSRLLPSLDRLQPWMIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQNYSHRFGYSRNRGSRYDETEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001348595.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348595.3

ORF Insert Sequence:

ATGGGAAACG ACGGCGACGG TGCCAGTGGC TCCAAGCCAA ATGGTTCCGT CTCGAGGCTC 
TTACCATCAC TCGATCGATT ACAGCCATGG ATGATACCAA TCGCCGCATC GGAAATTATA 
CCATCGCCGA TACATCCTAG AAAAATTTCA ATAAATGACC GCCTTTTTGA AATTCGCACG 
CCAAGACCCG GCGAAATGAT AAAAGAAGCG GTTCAGATGG CTGTTTGGCC AGCCTTACCA 
AGACTAGCTG CAGAAGAGGC CCGAAGAGAG CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG 
AAGACAACAC TTTCATCCAA TGCACCTGTC AAAGAAAAGG GTCCGTCGTC CCTATTCATC 
TTCGCCGAGG ACAACATCAT TCGTCGAAAC GCGAAGGCCA TCATAGAATG GGGACCGTTC 
GAATACTTCA TCTTATTGAC AATTATAGGC AACTGTGTGG TACTATCCAT GGAGCAACAT 
CTGCCGAAAA ACGACAAAAA GGCACTGTCG GAATGGCTGG AACGAACCGA ACCATATTTT 
ATGGGAATTT TTTGTTTAGA ATGTGTTTTG AAAGTCATAG CGTTTGGTTT TGCATTACAT 
AAAGGATCAT ATCTTCGATC AGGATGGAAT ATCATGGATT TTATTGTAGT AGTTTCCGGT 
GTCGTCACCA TGTTACCATT CTCTCCGGCA ACACAGACCG CCAACCAACC GGTGGATTCT 
GTCGATCTTC GAACATTGCG AGCTGTTCGA GTGCTTCGAC CGCTGAAACT GGTTTCTGGA 
ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG ATCTTGTGTG CTATGGCACC ATTGTTGCAA 
ATTGGATTAC TTGTGTTGTT TGCCATTATC ATCTTCGCCA TCATCGGACT GGAATTTTAT 
TCGGGTGCAT TTCACAGCGC TTGCTACAAC GAACGCGGTG AAATTGAAAA TGTTTCCGAG 
AGGCCAATGC CGTGCACCAA CAAAACGTCT CCAATGGGTG TTTATAACTG TGATGTAAAA 
GGAACGACTT GTTTGCAAAA ATGGATAGGT CCTAATTATG GAATTACTTC TTTCGACAAT 
ATCGGATTTG CTATGATAAC TGTGTTTCAG TGCATTACTA TGGAAGGGTG GACCACTGTA 
ATGTACTACA CAAACGATTC ACTTGGAAGC ACATACAACT GGGCTTACTT TATTCCGCTC 
ATTGTTCTAG GTTCTTTTTT CATGCTCAAT CTCGTTCTCG GAGTATTGTC TGGAGAGTTC 
GCCAAGGAAA GAGAACGTGT TGAAAATCGT CGGGAATTCT TGAAGCTTAG AAGGCAACAA 
CAAATTGAAC GAGAGCTTAA CGGATATCTA GAATGGATTT TAACAGCCGA AGAGGTGATT 
CTAAAAGAAG ATCGAACAAC TGAAGAAGAA AAAGCAGCGA TCATGGAAGC CAGACGGCGA 
GCCGCTAATA AAAAGTTAAA ACAAGCCTCA AAACAGCAGT CTACAGAAAC TGAAGAGGAC 
TTTGAGGAGG ACGAAGACGA AATGGAAGAG GAATACGTCG ACGAAGCAGA ACGTAAGAAA 
CGAGGATGTT GCCATTCAGT TGGAAAATTC ATCAAACAAC TGAGAATTCA AATCCGTATC 
ATGGTGAAAA CCCAAATCTT CTACTGGTCT GTCATCACAT TGGTCTTTCT AAACACTTGC 
TGCGTTGCCA GTGAACACTA TGGCCAACCA CAATGGTTCA CGGACTTTCT GAAATACGCC 
GAATTCGTGT TCCTCGGAAT TTTCGTAGTT GAAATGCTTT TGAAACTTTT CGCAATGGGA 
TCAAGAACAT ATTTTGCATC TAAATTCAAC CGTTTCGACT GTGTGGTCAT CGTTGGATCA 
GCTGCTGAAG TAATTTGGGC TGAAGTGTAT GGAGGATCAT TCGGTATTTC GGTGATGAGA 
GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC ACATCATACT GGGTGTCACT TCGTAATTTA 
GTCAGATCAC TCATGAACTC CATGAGGAGT ATTATTTCAC TTCTCTTCCT ACTTTTCTTG 
TTCATTCTCA TTTTCGCCCT TCTTGGAATG CAGTTGTTCG GAGGAAGATT CAACTTTCCA 
ACAATGCACC CTTACACACA CTTTGACACT TTTCCAGTTG CTCTCATTAC AGTATTCCAA 
ATCCTTACTG GAGAAGATTG GAACGAAGTC ATGTATCTCG CGATTGAATC ACAAGGAGGT 
ATCTACAGTG GTGGATGGCC ATACTCTATC TACTTTATTG TTTTAGTACT TTTCGGTAAC 
TACACTTTGT TGAATGTATT CTTGGCTATC GCCGTCGACA ACTTAGCCAA CGCTCAGGAA 
CTCACTGCAG CTGAAGAAGC AGACGAGAAA GCAAATGAAA TAGAGGAAGA ATCTGAAGAA 
CTTGACGAAC AGTACCAAGA AGGTGATCAT TGCACCATTG ATATGGAGGG AAAAACAGCT 
GGAGACATGT GTGCGGTTGC AAGAGCTATG GATGATTTGG ACGAAGAATG CGAAGAAGAA 
GAGTCACCAT TTGGAGGTCC AAAACCCATG GTTCCCTACA GTTCTATGTT CTTTCTCTCG 
CCAACTAATC CATTCCGAGT TCTGATTCAC AGTATCGTGT GCACCAAATA CTTTGAAATG 
ATGGTCATGA CCGTTATCTG TCTCTCATCG GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT 
GAAGAAAATC CGAGAAACAA AGTCCTACAG TATATGGATT ACTGTTTTAC TGGAGTTTTT 
GCATGTGAAA TGCTTCTAAA GTTGATTGAT CAAGGTATTC TTCTACATCC CGGATCATAT 
TGCCGCGATT TCTGGAATAT TCTTGATGGA ATTGTTGTAA CATGCGCACT TTTCGCATTT 
GGTTTTGCAG GTACCGAAGG AAGTGCTGGT AAAAATTTGA ACACAATCAA ATCGCTGAGG 
GTACTTCGAG TGCTTCGTCC ACTCAAAACA ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA 
TTCGATTGTG TTGTTAATTC ATTGAAGAAT GTATTCAACA TTCTTATTGT TTATTTTCTA 
TTTCAATTCA TTTTTGCTGT AATCGCAGTG CAGCTTTTCA ACGGGAAATT TTTTTTCTGC 
ACTGACAAGA ACCGAAAGTT CGCGAACACT TGCCACGGGC AGTTCTTTGT CTACGATAAT 
CAGAATGATC CACCACGGGT TGAACAACGT GAATGGCGAT TGAGACCGTT CAACTACGAC 
AACACAATCA ACGCAATGCT CACATTATTC GTTGTAACGA CAGGAGAAGG ATGGCCTGGC 
ATTCGGCAGA ACAGTATGGA CACAACGTTC GAAGATCAGG GTCCAAGTCC ATTTTTTCGG 
GTTGAAGTGG CTCTATTCTA TGTGATGTTT TTCATCGTCT TTCCATTTTT CTTCGTCAAC 
ATTTTCGTCG CGTTGATTAT CATCACGTTT CAAGAACAAG GAGAAGCAGA GTTAAGCGAA 
GGAGACTTGG ACAAGAACCA AAAACAATGT ATCGATTTTG CACTGAATGC TCGGCCAAGA 
TCACTATTCA TGCCGGAAGA CAAGAACTCT ACAAAGTATA GAATATGGAG GCTGGTTACA 
TCACCGCCGT TTGAATACTT TATCATGACA ATGATTTGCT GCAACACGCT CATTCTGATG 
ATGAAGTACT ACAATAATCC ATTGTTTTAT GAAGAAATTT TGAGGCTTTT CAATACCGCA 
TTAACGGCCG TGTTTACAGT GGAGAGTATA TTGAAGATTT TGGCATTCGG TGTTAGAAAT 
TACTTTCGTG ATGGTTGGAA TCGTTTCGAT TTCGTCACCG TCGTCGGTTC TATCACTGAT 
GCGCTGGTCA CAGAGTTCGG AGGTCATTTT GTGTCGCTTG GATTTCTTCG ACTGTTCCGA 
GCAGCTCGTC TCATTCGTCT CCTGCAGCAA GGATATACCA TCCGAATCCT ACTCTGGACG 
TTCGTTCAAA GTTTCAAAGC TCTACCGTAT GTGTGTCTTT TGATTGGAAT GCTCTTCTTC 
ATTTACGCAA TTGTTGGAAT GCAAGTATTC GGGAATATCT GGTTGAATGC TGCCACCGAA 
ATCAACAGAC ACAACAATTT CCAGTCTTTC TTCAACGCAG TTATTTTACT CTTCCGATGC 
GCGACTGGAG AAGGCTGGCA GGATATTATG ATGGCTGCAG TTCAAGGGAA AGATTGCGCA 
CGAGCCGGCT CTGCTGAAAT AAACTTTGAA AAAGGCCAAA CTTGCGGTAG CAACGTTTCA 
TATGCCTACT TCACAAGTTT CGTTTTCCTC TCTTCTTTCT TGATGCTCAA TTTATTCGTT 
GCCGTCATCA TGGATAATTT TGACTACCTC ACAAGAGATT CTTCCATTCT GGGTCCCCAT 
CATTTAGACG AGTTTATCAG AGTCTGGGCA GATTACGATC CAGCAGCAAC GGGCCGAATT 
CACTATTCCG AAATGTATGA GATGTTAAGA ATTATGGCAC CTCCTGTCGG TTTTGGAAAG 
AAATGTCCAT ACCGATTGGC TTATAAACAT CTAATCCGAA TGAACATGCC GGTAGCAGAG 
GACGGAACTG TGCACTTTAC AACTACCCTG TTCGCACTGA TTCGAGAAAG TTTGAGCATA 
AAAATGAGAC CGGTGGAAGA GATGGATGAA GCTGATGAGG AATTAAGATT GACATTAAAG 
AAAATCTGGC CACTGAAAGC AAAGAAAAAC ATGGTTGACT TGGTTGTTCC ACCGAATCAC 
GAACTTTGCT TCCAAAAGCT CACAGTGGGA AAAATCTACG CAGGACTTTT GATTCTCGAA 
AACTATCGAG CTCGAAAAAG CGGGACGGAG GTCGGTGGAC AAGGATTGTT TGGCGGAGGC 
TTACGATCGT TGGTCGCTGC AGCGAAAGCT GCCGAATCGC AACATTCTTC CCACACTCCA 
CAACCGCCAG AGGAAACCAC TCCGATAATT CCACAACATG CACAGCAGTT CTCGGCAGCA 
CCGACGATGA GCGCACAAGG ATCCTTGCAA CAAATGCAGG GAACGAGTAG TGGCGGTGGT 
CAACGTCCCT ATTCACTCTT CAATAGCTTC GTCGACACTA TCAAATCTGG AAAACAGGAT 
GGAGACGTCA CAGATGTGCA ATATCAAAGT GTTGATCAGC AGCACGAAAA GATGAACTCG 
ACTGGCAGGC GTTTGTCGGA TATGTTCTCG AAAATTCGAC GAGGCACATC AGCCGACCAC 
AATCCGCATC AGAACTATTC GCACCGCTTT GGTTACAGTC GCAATAGAGG TTCTCGATAT 
GATGAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_171638.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741732.2 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa355208
Clone ID Related Accession (Same CDS sequence)	NM_171638.2 , NM_171638.3
Accession Version	NM_171638.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Aug 9, 2005 this sequence version replaced NM_171638.1. COMPLETENESS: incomplete on both ends.

ATGATACCAA TCGCCGCATC GGAAATTATA CCATCGCCGA TACATCCTAG AAAAATTTCA 
ATAAATGACC GCCTTTTTGA AATTCGCACG CCAAGACCCG GCGAAATGAT AAAAGAAGCG 
GTTCAGATGG CTGTTTGGCC AGCCTTACCA AGACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741732.2
CDS	1..6084
Translation	MIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_171638.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171638.2

ORF Insert Sequence:

ATGATACCAA TCGCCGCATC GGAAATTATA CCATCGCCGA TACATCCTAG AAAAATTTCA 
ATAAATGACC GCCTTTTTGA AATTCGCACG CCAAGACCCG GCGAAATGAT AAAAGAAGCG 
GTTCAGATGG CTGTTTGGCC AGCCTTACCA AGACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_171638.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_741732.2 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), mRNA.

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CloneID	OCa355208
Clone ID Related Accession (Same CDS sequence)	NM_171638.2 , NM_171638.3
Accession Version	NM_171638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_171638.2.

ATGATACCAA TCGCCGCATC GGAAATTATA CCATCGCCGA TACATCCTAG AAAAATTTCA 
ATAAATGACC GCCTTTTTGA AATTCGCACG CCAAGACCCG GCGAAATGAT AAAAGAAGCG 
GTTCAGATGG CTGTTTGGCC AGCCTTACCA AGACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741732.2
CDS	57..6140
Translation	MIPIAASEIIPSPIHPRKISINDRLFEIRTPRPGEMIKEAVQMAVWPALPRLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_171638.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171638.3

ORF Insert Sequence:

ATGATACCAA TCGCCGCATC GGAAATTATA CCATCGCCGA TACATCCTAG AAAAATTTCA 
ATAAATGACC GCCTTTTTGA AATTCGCACG CCAAGACCCG GCGAAATGAT AAAAGAAGCG 
GTTCAGATGG CTGTTTGGCC AGCCTTACCA AGACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368494.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355464.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa698993
Clone ID Related Accession (Same CDS sequence)	NM_001368494.3 , NM_001368494.1
Accession Version	NM_001368494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368494.2. COMPLETENESS: incomplete on the 5' end.

ATGAACCCAC TTCATCGGTC ATCATACAGA CCAACAAAGA CAAAGTATTA TCTACCATTA 
TCCAGAACTC CTTCCCATCG AAAACCAAAG ACGAGTGCAA CATCGACGTA TTCCGCAGAA 
AAATCAGGAG ATGAGTCTGT AACGATCAAC ATTGAATTTT CGTATCGATC GCAGTCTTCC 
GGGACGTCAA CCCTTCAAAA AAACAGGAAA CCTTCAGAAC CTCCAAAGTC AAAACGATCA 
AGCAGGGATG ATAATTCAAA TGGGTATGCA GGGTATGAAG ATGCAACGCC ACGACCCTCA 
ATCGACTTTC CAACTGCAGT TATTGAAGCT GCCCGTCGGG CGTCAGTTCT CGGTCGATCA 
GCTGCCGAAT CGTTATCTGC TCAAGAAGCA GCATCGAGCA GCGAACGGGG AGAGCACACT 
AACAGTCGCT CCCCATCGAC CAGCTATTCG AGTTGTATCG ACGACGAACG AAAGTTCTCG 
AGTCCTCATC GTAGACGAGT AGTCGATGTC AGCGATCATA AGACATGTGC ACTGTTGATG 
ACGAGGATGA AAGAGGCAAG TAGACAGCTG CCATCGCCGA GCCAACTAGC TGCAGAAGAG 
GCCCGAAGAG AGCAAAAAGC AGAGTCTGGC ACATTCGTCA GGAAGACAAC ACTTTCATCC 
AATGCACCTG TCAAAGAAAA GGGTCCGTCG TCCCTATTCA TCTTCGCCGA GGACAACATC 
ATTCGTCGAA ACGCGAAGGC CATCATAGAA TGGGGACCGT TCGAATACTT CATCTTATTG 
ACAATTATAG GCAACTGTGT GGTACTATCC ATGGAGCAAC ATCTGCCGAA AAACGACAAA 
AAGGCACTGT CGGAATGGCT GGAACGAACC GAACCATATT TTATGGGAAT TTTTTGTTTA 
GAATGTGTTT TGAAAGTCAT AGCGTTTGGT TTTGCATTAC ATAAAGGATC ATATCTTCGA 
TCAGGATGGA ATATCATGGA TTTTATTGTA GTAGTTTCCG GTGTCGTCAC CATGTTACCA 
TTCTCTCCGG CAACACAGAC CGCCAACCAA CCGGTGGATT CTGTCGATCT TCGAACATTG 
CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA CTGGTTTCTG GAATTCCAAG TTTGCAAGTT 
GTCCTCAAGT CGATCTTGTG TGCTATGGCA CCATTGTTGC AAATTGGATT ACTTGTGTTG 
TTTGCCATTA TCATCTTCGC CATCATCGGA CTGGAATTTT ATTCGGGTGC ATTTCACAGC 
GCTTGCTACA ACGAACGCGG TGAAATTGAA AATGTTTCCG AGAGGCCAAT GCCGTGCACC 
AACAAAACGT CTCCAATGGG TGTTTATAAC TGTGATGTAA AAGGAACGAC TTGTTTGCAA 
AAATGGATAG GTCCTAATTA TGGAATTACT TCTTTCGACA ATATCGGATT TGCTATGATA 
ACTGTGTTTC AGTGCATTAC TATGGAAGGG TGGACCACTG TAATGTACTA CACAAACGAT 
TCACTTGGAA GCACATACAA CTGGGCTTAC TTTATTCCGC TCATTGTTCT AGGTTCTTTT 
TTCATGCTCA ATCTCGTTCT CGGAGTATTG TCTGGAGAGT TCGCCAAGGA AAGAGAACGT 
GTTGAAAATC GTCGGGAATT CTTGAAGCTT AGAAGGCAAC AACAAATTGA ACGAGAGCTT 
AACGGATATC TAGAATGGAT TTTAACAGCC GAAGAGGTGA TTCTAAAAGA AGATCGAACA 
ACTGAAGAAG AAAAAGCAGC GATCATGGAA GCCAGACGGC GAGCCGCTAA TAAAAAGTTA 
AAACAAGCCT CAAAACAGCA GTCTACAGAA ACTGAAGAGG ACTTTGAGGA GGACGAAGAC 
GAAATGGAAG AGGAATACGT CGACGAAGGT GGAACAGTAG AAGACGAATT CGCAGAACGT 
AAGAAACGAG GATGTTGCCA TTCAGTTGGA AAATTCATCA AACAACTGAG AATTCAAATC 
CGTATCATGG TGAAAACCCA AATCTTCTAC TGGTCTGTCA TCACATTGGT CTTTCTAAAC 
ACTTGCTGCG TTGCCAGTGA ACACTATGGC CAACCACAAT GGTTCACGGA CTTTCTGAAA 
TACGCCGAAT TCGTGTTCCT CGGAATTTTC GTAGTTGAAA TGCTTTTGAA ACTTTTCGCA 
ATGGGATCAA GAACATATTT TGCATCTAAA TTCAACCGTT TCGACTGTGT GGTCATCGTT 
GGATCAGCTG CTGAAGTAAT TTGGGCTGAA GTGTATGGAG GATCATTCGG TATTTCGGTG 
ATGAGAGCGC TGAGACTTCT TCGAATTTTC AAGCTCACAT CATACTGGGT GTCACTTCGT 
AATTTAGTCA GATCACTCAT GAACTCCATG AGGAGTATTA TTTCACTTCT CTTCCTACTT 
TTCTTGTTCA TTCTCATTTT CGCCCTTCTT GGAATGCAGT TGTTCGGAGG AAGATTCAAC 
TTTCCAACAA TGCACCCTTA CACACACTTT GACACTTTTC CAGTTGCTCT CATTACAGTA 
TTCCAAATCC TTACTGGAGA AGATTGGAAC GAAGTCATGT ATCTCGCGAT TGAATCACAA 
GGAGGTATCT ACAGTGGTGG ATGGCCATAC TCTATCTACT TTATTGTTTT AGTACTTTTC 
GGTAACTACA CTTTGTTGAA TGTATTCTTG GCTATCGCCG TCGACAACTT AGCCAACGCT 
CAGGAACTCA CTGCAGCTGA AGAAGCAGAC GAGAAAGCAA ATGAAATAGA GGAAGAATCT 
GAAGAACTTG ACGAACAGTA CCAAGAAGGT GATCATTGCA CCATTGATAT GGAGGGAAAA 
ACAGCTGGAG ACATGTGTGC GGTTGCAAGA GCTATGGATG ATTTGGACGA AGAATGCGAA 
GAAGAAGAGT CACCATTTGG AGGTCCAAAA CCCATGGTTC CCTACAGTTC TATGTTCTTT 
CTCTCGCCAA CTAATCCATT CCGAGTTCTG ATTCACAGTA TCGTGTGCAC CAAATACTTT 
GAAATGATGG TCATGACCGT TATCTGTCTC TCATCGGTTT CTCTAGCCGC TGAAGATCCA 
GTCGATGAAG AAAATCCGAG AAACAAAGTC CTACAGTATA TGGATTACTG TTTTACTGGA 
GTTTTTGCAT GTGAAATGCT TCTAAAGTTG ATTGATCAAG GTATTCTTCT ACATCCCGGA 
TCATATTGCC GCGATTTCTG GAATATTCTT GATGGAATTG TTGTAACATG CGCACTTTTC 
GCATTTGGTT TTGCAGGTAC CGAAGGAAGT GCTGGTAAAA ATTTGAACAC AATCAAATCG 
CTGAGGGTAC TTCGAGTGCT TCGTCCACTC AAAACAATCA AAAGAATTCC AAAGCTCAAG 
GCTGTATTCG ATTGTGTTGT TAATTCATTG AAGAATGTAT TCAACATTCT TATTGTTTAT 
TTTCTATTTC AATTCATTTT TGCTGTAATC GCAGTGCAGC TTTTCAACGG GAAATTTTTT 
TTCTGCACTG ACAAGAACCG AAAGTTCGCG AACACTTGCC ACGGGCAGTT CTTTGTCTAC 
GATAATCAGA ATGATCCACC ACGGGTTGAA CAACGTGAAT GGCGATTGAG ACCGTTCAAC 
TACGACAACA CAATCAACGC AATGCTCACA TTATTCGTTG TAACGACAGG AGAAGGATGG 
CCTGGCATTC GGCAGAACAG TATGGACACA ACGTTCGAAG ATCAGGGTCC AAGTCCATTT 
TTTCGGGTTG AAGTGGCTCT ATTCTATGTG ATGTTTTTCA TCGTCTTTCC ATTTTTCTTC 
GTCAACATTT TCGTCGCGTT GATTATCATC ACGTTTCAAG AACAAGGAGA AGCAGAGTTA 
AGCGAAGGAG ACTTGGACAA GAACCAAAAA CAATGTATCG ATTTTGCACT GAATGCTCGG 
CCAAGATCAC TATTCATGCC GGAAGACAAG AACTCTACAA AGTATAGAAT ATGGAGGCTG 
GTTACATCAC CGCCGTTTGA ATACTTTATC ATGACAATGA TTTGCTGCAA CACGCTCATT 
CTGATGATGA AGTACTACAA TAATCCATTG TTTTATGAAG AAATTTTGAG GCTTTTCAAT 
ACCGCATTAA CGGCCGTGTT TACAGTGGAG AGTATATTGA AGATTTTGGC ATTCGGTGTT 
AGAAATTACT TTCGTGATGG TTGGAATCGT TTCGATTTCG TCACCGTCGT CGGTTCTATC 
ACTGATGCGC TGGTCACAGA GTTCGGAGGT CATTTTGTGT CGCTTGGATT TCTTCGACTG 
TTCCGAGCAG CTCGTCTCAT TCGTCTCCTG CAGCAAGGAT ATACCATCCG AATCCTACTC 
TGGACGTTCG TTCAAAGTTT CAAAGCTCTA CCGTATGTGT GTCTTTTGAT TGGAATGCTC 
TTCTTCATTT ACGCAATTGT TGGAATGCAA GTATTCGGGA ATATCTGGTT GAATGCTGCC 
ACCGAAATCA ACAGACACAA CAATTTCCAG TCTTTCTTCA ACGCAGTTAT TTTACTCTTC 
CGATGCGCGA CTGGAGAAGG CTGGCAGGAT ATTATGATGG CTGCAGTTCA AGGGAAAGAT 
TGCGCACGAG CCGGCTCTGC TGAAATAAAC TTTGAAAAAG GCCAAACTTG CGGTAGCAAC 
GTTTCATATG CCTACTTCAC AAGTTTCGTT TTCCTCTCTT CTTTCTTGAT GCTCAATTTA 
TTCGTTGCCG TCATCATGGA TAATTTTGAC TACCTCACAA GAGATTCTTC CATTCTGGGT 
CCCCATCATT TAGACGAGTT TATCAGAGTC TGGGCAGATT ACGATCCAGC AGCAACGGGC 
CGAATTCACT ATTCCGAAAT GTATGAGATG TTAAGAATTA TGGCACCTCC TGTCGGTTTT 
GGAAAGAAAT GTCCATACCG ATTGGCTTAT AAACATCTAA TCCGAATGAA CATGCCGGTA 
GCAGAGGACG GAACTGTGCA CTTTACAACT ACCCTGTTCG CACTGATTCG AGAAAGTTTG 
AGCATAAAAA TGAGACCGGT GGAAGAGATG GATGAAGCTG ATGAGGAATT AAGATTGACA 
TTAAAGAAAA TCTGGCCACT GAAAGCAAAG AAAAACATGG TTGACTTGGT TGTTCCACCG 
AATCACGAAC TTTGCTTCCA AAAGCTCACA GTGGGAAAAA TCTACGCAGG ACTTTTGATT 
CTCGAAAACT ATCGAGCTCG AAAAAGCGGG ACGGAGGTCG GTGGACAAGG ATTGTTTGGC 
GGAGGCTTAC GATCGTTGGT CGCTGCAGCG AAAGCTGCCG AATCGCAACA TTCTTCCCAC 
ACTCCACAAC CGCCAGAGGA AACCACTCCG ATAATTCCAC AACATGCACA GCAGTTCTCG 
GCAGCACCGA CGATGAGCGC ACAAGGATCC TTGCAACAAA TGCAGGGAAC GAGTAGTGGC 
GGTGGTCAAC GTCCCTATTC ACTCTTCAAT AGCTTCGTCG ACACTATCAA ATCTGGAAAA 
CAGGATGGAG ACGTCACAGA TGTGCAATAT CAAAGTGTTG ATCAGCAGCA CGAAAAGATG 
AACTCGACTG GCAGGCGTTT GTCGGATATG TTCTCGAAAA TTCGACGAGG CACATCAGCC 
GACCACAATC CGCATCAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355464.1
CDS	1..6516
Translation	MNPLHRSSYRPTKTKYYLPLSRTPSHRKPKTSATSTYSAEKSGDESVTINIEFSYRSQSSGTSTLQKNRKPSEPPKSKRSSRDDNSNGYAGYEDATPRPSIDFPTAVIEAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368494.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368494.3

ORF Insert Sequence:

ATGAACCCAC TTCATCGGTC ATCATACAGA CCAACAAAGA CAAAGTATTA TCTACCATTA 
TCCAGAACTC CTTCCCATCG AAAACCAAAG ACGAGTGCAA CATCGACGTA TTCCGCAGAA 
AAATCAGGAG ATGAGTCTGT AACGATCAAC ATTGAATTTT CGTATCGATC GCAGTCTTCC 
GGGACGTCAA CCCTTCAAAA AAACAGGAAA CCTTCAGAAC CTCCAAAGTC AAAACGATCA 
AGCAGGGATG ATAATTCAAA TGGGTATGCA GGGTATGAAG ATGCAACGCC ACGACCCTCA 
ATCGACTTTC CAACTGCAGT TATTGAAGCT GCCCGTCGGG CGTCAGTTCT CGGTCGATCA 
GCTGCCGAAT CGTTATCTGC TCAAGAAGCA GCATCGAGCA GCGAACGGGG AGAGCACACT 
AACAGTCGCT CCCCATCGAC CAGCTATTCG AGTTGTATCG ACGACGAACG AAAGTTCTCG 
AGTCCTCATC GTAGACGAGT AGTCGATGTC AGCGATCATA AGACATGTGC ACTGTTGATG 
ACGAGGATGA AAGAGGCAAG TAGACAGCTG CCATCGCCGA GCCAACTAGC TGCAGAAGAG 
GCCCGAAGAG AGCAAAAAGC AGAGTCTGGC ACATTCGTCA GGAAGACAAC ACTTTCATCC 
AATGCACCTG TCAAAGAAAA GGGTCCGTCG TCCCTATTCA TCTTCGCCGA GGACAACATC 
ATTCGTCGAA ACGCGAAGGC CATCATAGAA TGGGGACCGT TCGAATACTT CATCTTATTG 
ACAATTATAG GCAACTGTGT GGTACTATCC ATGGAGCAAC ATCTGCCGAA AAACGACAAA 
AAGGCACTGT CGGAATGGCT GGAACGAACC GAACCATATT TTATGGGAAT TTTTTGTTTA 
GAATGTGTTT TGAAAGTCAT AGCGTTTGGT TTTGCATTAC ATAAAGGATC ATATCTTCGA 
TCAGGATGGA ATATCATGGA TTTTATTGTA GTAGTTTCCG GTGTCGTCAC CATGTTACCA 
TTCTCTCCGG CAACACAGAC CGCCAACCAA CCGGTGGATT CTGTCGATCT TCGAACATTG 
CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA CTGGTTTCTG GAATTCCAAG TTTGCAAGTT 
GTCCTCAAGT CGATCTTGTG TGCTATGGCA CCATTGTTGC AAATTGGATT ACTTGTGTTG 
TTTGCCATTA TCATCTTCGC CATCATCGGA CTGGAATTTT ATTCGGGTGC ATTTCACAGC 
GCTTGCTACA ACGAACGCGG TGAAATTGAA AATGTTTCCG AGAGGCCAAT GCCGTGCACC 
AACAAAACGT CTCCAATGGG TGTTTATAAC TGTGATGTAA AAGGAACGAC TTGTTTGCAA 
AAATGGATAG GTCCTAATTA TGGAATTACT TCTTTCGACA ATATCGGATT TGCTATGATA 
ACTGTGTTTC AGTGCATTAC TATGGAAGGG TGGACCACTG TAATGTACTA CACAAACGAT 
TCACTTGGAA GCACATACAA CTGGGCTTAC TTTATTCCGC TCATTGTTCT AGGTTCTTTT 
TTCATGCTCA ATCTCGTTCT CGGAGTATTG TCTGGAGAGT TCGCCAAGGA AAGAGAACGT 
GTTGAAAATC GTCGGGAATT CTTGAAGCTT AGAAGGCAAC AACAAATTGA ACGAGAGCTT 
AACGGATATC TAGAATGGAT TTTAACAGCC GAAGAGGTGA TTCTAAAAGA AGATCGAACA 
ACTGAAGAAG AAAAAGCAGC GATCATGGAA GCCAGACGGC GAGCCGCTAA TAAAAAGTTA 
AAACAAGCCT CAAAACAGCA GTCTACAGAA ACTGAAGAGG ACTTTGAGGA GGACGAAGAC 
GAAATGGAAG AGGAATACGT CGACGAAGGT GGAACAGTAG AAGACGAATT CGCAGAACGT 
AAGAAACGAG GATGTTGCCA TTCAGTTGGA AAATTCATCA AACAACTGAG AATTCAAATC 
CGTATCATGG TGAAAACCCA AATCTTCTAC TGGTCTGTCA TCACATTGGT CTTTCTAAAC 
ACTTGCTGCG TTGCCAGTGA ACACTATGGC CAACCACAAT GGTTCACGGA CTTTCTGAAA 
TACGCCGAAT TCGTGTTCCT CGGAATTTTC GTAGTTGAAA TGCTTTTGAA ACTTTTCGCA 
ATGGGATCAA GAACATATTT TGCATCTAAA TTCAACCGTT TCGACTGTGT GGTCATCGTT 
GGATCAGCTG CTGAAGTAAT TTGGGCTGAA GTGTATGGAG GATCATTCGG TATTTCGGTG 
ATGAGAGCGC TGAGACTTCT TCGAATTTTC AAGCTCACAT CATACTGGGT GTCACTTCGT 
AATTTAGTCA GATCACTCAT GAACTCCATG AGGAGTATTA TTTCACTTCT CTTCCTACTT 
TTCTTGTTCA TTCTCATTTT CGCCCTTCTT GGAATGCAGT TGTTCGGAGG AAGATTCAAC 
TTTCCAACAA TGCACCCTTA CACACACTTT GACACTTTTC CAGTTGCTCT CATTACAGTA 
TTCCAAATCC TTACTGGAGA AGATTGGAAC GAAGTCATGT ATCTCGCGAT TGAATCACAA 
GGAGGTATCT ACAGTGGTGG ATGGCCATAC TCTATCTACT TTATTGTTTT AGTACTTTTC 
GGTAACTACA CTTTGTTGAA TGTATTCTTG GCTATCGCCG TCGACAACTT AGCCAACGCT 
CAGGAACTCA CTGCAGCTGA AGAAGCAGAC GAGAAAGCAA ATGAAATAGA GGAAGAATCT 
GAAGAACTTG ACGAACAGTA CCAAGAAGGT GATCATTGCA CCATTGATAT GGAGGGAAAA 
ACAGCTGGAG ACATGTGTGC GGTTGCAAGA GCTATGGATG ATTTGGACGA AGAATGCGAA 
GAAGAAGAGT CACCATTTGG AGGTCCAAAA CCCATGGTTC CCTACAGTTC TATGTTCTTT 
CTCTCGCCAA CTAATCCATT CCGAGTTCTG ATTCACAGTA TCGTGTGCAC CAAATACTTT 
GAAATGATGG TCATGACCGT TATCTGTCTC TCATCGGTTT CTCTAGCCGC TGAAGATCCA 
GTCGATGAAG AAAATCCGAG AAACAAAGTC CTACAGTATA TGGATTACTG TTTTACTGGA 
GTTTTTGCAT GTGAAATGCT TCTAAAGTTG ATTGATCAAG GTATTCTTCT ACATCCCGGA 
TCATATTGCC GCGATTTCTG GAATATTCTT GATGGAATTG TTGTAACATG CGCACTTTTC 
GCATTTGGTT TTGCAGGTAC CGAAGGAAGT GCTGGTAAAA ATTTGAACAC AATCAAATCG 
CTGAGGGTAC TTCGAGTGCT TCGTCCACTC AAAACAATCA AAAGAATTCC AAAGCTCAAG 
GCTGTATTCG ATTGTGTTGT TAATTCATTG AAGAATGTAT TCAACATTCT TATTGTTTAT 
TTTCTATTTC AATTCATTTT TGCTGTAATC GCAGTGCAGC TTTTCAACGG GAAATTTTTT 
TTCTGCACTG ACAAGAACCG AAAGTTCGCG AACACTTGCC ACGGGCAGTT CTTTGTCTAC 
GATAATCAGA ATGATCCACC ACGGGTTGAA CAACGTGAAT GGCGATTGAG ACCGTTCAAC 
TACGACAACA CAATCAACGC AATGCTCACA TTATTCGTTG TAACGACAGG AGAAGGATGG 
CCTGGCATTC GGCAGAACAG TATGGACACA ACGTTCGAAG ATCAGGGTCC AAGTCCATTT 
TTTCGGGTTG AAGTGGCTCT ATTCTATGTG ATGTTTTTCA TCGTCTTTCC ATTTTTCTTC 
GTCAACATTT TCGTCGCGTT GATTATCATC ACGTTTCAAG AACAAGGAGA AGCAGAGTTA 
AGCGAAGGAG ACTTGGACAA GAACCAAAAA CAATGTATCG ATTTTGCACT GAATGCTCGG 
CCAAGATCAC TATTCATGCC GGAAGACAAG AACTCTACAA AGTATAGAAT ATGGAGGCTG 
GTTACATCAC CGCCGTTTGA ATACTTTATC ATGACAATGA TTTGCTGCAA CACGCTCATT 
CTGATGATGA AGTACTACAA TAATCCATTG TTTTATGAAG AAATTTTGAG GCTTTTCAAT 
ACCGCATTAA CGGCCGTGTT TACAGTGGAG AGTATATTGA AGATTTTGGC ATTCGGTGTT 
AGAAATTACT TTCGTGATGG TTGGAATCGT TTCGATTTCG TCACCGTCGT CGGTTCTATC 
ACTGATGCGC TGGTCACAGA GTTCGGAGGT CATTTTGTGT CGCTTGGATT TCTTCGACTG 
TTCCGAGCAG CTCGTCTCAT TCGTCTCCTG CAGCAAGGAT ATACCATCCG AATCCTACTC 
TGGACGTTCG TTCAAAGTTT CAAAGCTCTA CCGTATGTGT GTCTTTTGAT TGGAATGCTC 
TTCTTCATTT ACGCAATTGT TGGAATGCAA GTATTCGGGA ATATCTGGTT GAATGCTGCC 
ACCGAAATCA ACAGACACAA CAATTTCCAG TCTTTCTTCA ACGCAGTTAT TTTACTCTTC 
CGATGCGCGA CTGGAGAAGG CTGGCAGGAT ATTATGATGG CTGCAGTTCA AGGGAAAGAT 
TGCGCACGAG CCGGCTCTGC TGAAATAAAC TTTGAAAAAG GCCAAACTTG CGGTAGCAAC 
GTTTCATATG CCTACTTCAC AAGTTTCGTT TTCCTCTCTT CTTTCTTGAT GCTCAATTTA 
TTCGTTGCCG TCATCATGGA TAATTTTGAC TACCTCACAA GAGATTCTTC CATTCTGGGT 
CCCCATCATT TAGACGAGTT TATCAGAGTC TGGGCAGATT ACGATCCAGC AGCAACGGGC 
CGAATTCACT ATTCCGAAAT GTATGAGATG TTAAGAATTA TGGCACCTCC TGTCGGTTTT 
GGAAAGAAAT GTCCATACCG ATTGGCTTAT AAACATCTAA TCCGAATGAA CATGCCGGTA 
GCAGAGGACG GAACTGTGCA CTTTACAACT ACCCTGTTCG CACTGATTCG AGAAAGTTTG 
AGCATAAAAA TGAGACCGGT GGAAGAGATG GATGAAGCTG ATGAGGAATT AAGATTGACA 
TTAAAGAAAA TCTGGCCACT GAAAGCAAAG AAAAACATGG TTGACTTGGT TGTTCCACCG 
AATCACGAAC TTTGCTTCCA AAAGCTCACA GTGGGAAAAA TCTACGCAGG ACTTTTGATT 
CTCGAAAACT ATCGAGCTCG AAAAAGCGGG ACGGAGGTCG GTGGACAAGG ATTGTTTGGC 
GGAGGCTTAC GATCGTTGGT CGCTGCAGCG AAAGCTGCCG AATCGCAACA TTCTTCCCAC 
ACTCCACAAC CGCCAGAGGA AACCACTCCG ATAATTCCAC AACATGCACA GCAGTTCTCG 
GCAGCACCGA CGATGAGCGC ACAAGGATCC TTGCAACAAA TGCAGGGAAC GAGTAGTGGC 
GGTGGTCAAC GTCCCTATTC ACTCTTCAAT AGCTTCGTCG ACACTATCAA ATCTGGAAAA 
CAGGATGGAG ACGTCACAGA TGTGCAATAT CAAAGTGTTG ATCAGCAGCA CGAAAAGATG 
AACTCGACTG GCAGGCGTTT GTCGGATATG TTCTCGAAAA TTCGACGAGG CACATCAGCC 
GACCACAATC CGCATCAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368494.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355464.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa698993
Clone ID Related Accession (Same CDS sequence)	NM_001368494.3 , NM_001368494.1
Accession Version	NM_001368494.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGAACCCAC TTCATCGGTC ATCATACAGA CCAACAAAGA CAAAGTATTA TCTACCATTA 
TCCAGAACTC CTTCCCATCG AAAACCAAAG ACGAGTGCAA CATCGACGTA TTCCGCAGAA 
AAATCAGGAG ATGAGTCTGT AACGATCAAC ATTGAATTTT CGTATCGATC GCAGTCTTCC 
GGGACGTCAA CCCTTCAAAA AAACAGGAAA CCTTCAGAAC CTCCAAAGTC AAAACGATCA 
AGCAGGGATG ATAATTCAAA TGGGTATGCA GGGTATGAAG ATGCAACGCC ACGACCCTCA 
ATCGACTTTC CAACTGCAGT TATTGAAGCT GCCCGTCGGG CGTCAGTTCT CGGTCGATCA 
GCTGCCGAAT CGTTATCTGC TCAAGAAGCA GCATCGAGCA GCGAACGGGG AGAGCACACT 
AACAGTCGCT CCCCATCGAC CAGCTATTCG AGTTGTATCG ACGACGAACG AAAGTTCTCG 
AGTCCTCATC GTAGACGAGT AGTCGATGTC AGCGATCATA AGACATGTGC ACTGTTGATG 
ACGAGGATGA AAGAGGCAAG TAGACAGCTG CCATCGCCGA GCCAACTAGC TGCAGAAGAG 
GCCCGAAGAG AGCAAAAAGC AGAGTCTGGC ACATTCGTCA GGAAGACAAC ACTTTCATCC 
AATGCACCTG TCAAAGAAAA GGGTCCGTCG TCCCTATTCA TCTTCGCCGA GGACAACATC 
ATTCGTCGAA ACGCGAAGGC CATCATAGAA TGGGGACCGT TCGAATACTT CATCTTATTG 
ACAATTATAG GCAACTGTGT GGTACTATCC ATGGAGCAAC ATCTGCCGAA AAACGACAAA 
AAGGCACTGT CGGAATGGCT GGAACGAACC GAACCATATT TTATGGGAAT TTTTTGTTTA 
GAATGTGTTT TGAAAGTCAT AGCGTTTGGT TTTGCATTAC ATAAAGGATC ATATCTTCGA 
TCAGGATGGA ATATCATGGA TTTTATTGTA GTAGTTTCCG GTGTCGTCAC CATGTTACCA 
TTCTCTCCGG CAACACAGAC CGCCAACCAA CCGGTGGATT CTGTCGATCT TCGAACATTG 
CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA CTGGTTTCTG GAATTCCAAG TTTGCAAGTT 
GTCCTCAAGT CGATCTTGTG TGCTATGGCA CCATTGTTGC AAATTGGATT ACTTGTGTTG 
TTTGCCATTA TCATCTTCGC CATCATCGGA CTGGAATTTT ATTCGGGTGC ATTTCACAGC 
GCTTGCTACA ACGAACGCGG TGAAATTGAA AATGTTTCCG AGAGGCCAAT GCCGTGCACC 
AACAAAACGT CTCCAATGGG TGTTTATAAC TGTGATGTAA AAGGAACGAC TTGTTTGCAA 
AAATGGATAG GTCCTAATTA TGGAATTACT TCTTTCGACA ATATCGGATT TGCTATGATA 
ACTGTGTTTC AGTGCATTAC TATGGAAGGG TGGACCACTG TAATGTACTA CACAAACGAT 
TCACTTGGAA GCACATACAA CTGGGCTTAC TTTATTCCGC TCATTGTTCT AGGTTCTTTT 
TTCATGCTCA ATCTCGTTCT CGGAGTATTG TCTGGAGAGT TCGCCAAGGA AAGAGAACGT 
GTTGAAAATC GTCGGGAATT CTTGAAGCTT AGAAGGCAAC AACAAATTGA ACGAGAGCTT 
AACGGATATC TAGAATGGAT TTTAACAGCC GAAGAGGTGA TTCTAAAAGA AGATCGAACA 
ACTGAAGAAG AAAAAGCAGC GATCATGGAA GCCAGACGGC GAGCCGCTAA TAAAAAGTTA 
AAACAAGCCT CAAAACAGCA GTCTACAGAA ACTGAAGAGG ACTTTGAGGA GGACGAAGAC 
GAAATGGAAG AGGAATACGT CGACGAAGGT GGAACAGTAG AAGACGAATT CGCAGAACGT 
AAGAAACGAG GATGTTGCCA TTCAGTTGGA AAATTCATCA AACAACTGAG AATTCAAATC 
CGTATCATGG TGAAAACCCA AATCTTCTAC TGGTCTGTCA TCACATTGGT CTTTCTAAAC 
ACTTGCTGCG TTGCCAGTGA ACACTATGGC CAACCACAAT GGTTCACGGA CTTTCTGAAA 
TACGCCGAAT TCGTGTTCCT CGGAATTTTC GTAGTTGAAA TGCTTTTGAA ACTTTTCGCA 
ATGGGATCAA GAACATATTT TGCATCTAAA TTCAACCGTT TCGACTGTGT GGTCATCGTT 
GGATCAGCTG CTGAAGTAAT TTGGGCTGAA GTGTATGGAG GATCATTCGG TATTTCGGTG 
ATGAGAGCGC TGAGACTTCT TCGAATTTTC AAGCTCACAT CATACTGGGT GTCACTTCGT 
AATTTAGTCA GATCACTCAT GAACTCCATG AGGAGTATTA TTTCACTTCT CTTCCTACTT 
TTCTTGTTCA TTCTCATTTT CGCCCTTCTT GGAATGCAGT TGTTCGGAGG AAGATTCAAC 
TTTCCAACAA TGCACCCTTA CACACACTTT GACACTTTTC CAGTTGCTCT CATTACAGTA 
TTCCAAATCC TTACTGGAGA AGATTGGAAC GAAGTCATGT ATCTCGCGAT TGAATCACAA 
GGAGGTATCT ACAGTGGTGG ATGGCCATAC TCTATCTACT TTATTGTTTT AGTACTTTTC 
GGTAACTACA CTTTGTTGAA TGTATTCTTG GCTATCGCCG TCGACAACTT AGCCAACGCT 
CAGGAACTCA CTGCAGCTGA AGAAGCAGAC GAGAAAGCAA ATGAAATAGA GGAAGAATCT 
GAAGAACTTG ACGAACAGTA CCAAGAAGGT GATCATTGCA CCATTGATAT GGAGGGAAAA 
ACAGCTGGAG ACATGTGTGC GGTTGCAAGA GCTATGGATG ATTTGGACGA AGAATGCGAA 
GAAGAAGAGT CACCATTTGG AGGTCCAAAA CCCATGGTTC CCTACAGTTC TATGTTCTTT 
CTCTCGCCAA CTAATCCATT CCGAGTTCTG ATTCACAGTA TCGTGTGCAC CAAATACTTT 
GAAATGATGG TCATGACCGT TATCTGTCTC TCATCGGTTT CTCTAGCCGC TGAAGATCCA 
GTCGATGAAG AAAATCCGAG AAACAAAGTC CTACAGTATA TGGATTACTG TTTTACTGGA 
GTTTTTGCAT GTGAAATGCT TCTAAAGTTG ATTGATCAAG GTATTCTTCT ACATCCCGGA 
TCATATTGCC GCGATTTCTG GAATATTCTT GATGGAATTG TTGTAACATG CGCACTTTTC 
GCATTTGGTT TTGCAGGTAC CGAAGGAAGT GCTGGTAAAA ATTTGAACAC AATCAAATCG 
CTGAGGGTAC TTCGAGTGCT TCGTCCACTC AAAACAATCA AAAGAATTCC AAAGCTCAAG 
GCTGTATTCG ATTGTGTTGT TAATTCATTG AAGAATGTAT TCAACATTCT TATTGTTTAT 
TTTCTATTTC AATTCATTTT TGCTGTAATC GCAGTGCAGC TTTTCAACGG GAAATTTTTT 
TTCTGCACTG ACAAGAACCG AAAGTTCGCG AACACTTGCC ACGGGCAGTT CTTTGTCTAC 
GATAATCAGA ATGATCCACC ACGGGTTGAA CAACGTGAAT GGCGATTGAG ACCGTTCAAC 
TACGACAACA CAATCAACGC AATGCTCACA TTATTCGTTG TAACGACAGG AGAAGGATGG 
CCTGGCATTC GGCAGAACAG TATGGACACA ACGTTCGAAG ATCAGGGTCC AAGTCCATTT 
TTTCGGGTTG AAGTGGCTCT ATTCTATGTG ATGTTTTTCA TCGTCTTTCC ATTTTTCTTC 
GTCAACATTT TCGTCGCGTT GATTATCATC ACGTTTCAAG AACAAGGAGA AGCAGAGTTA 
AGCGAAGGAG ACTTGGACAA GAACCAAAAA CAATGTATCG ATTTTGCACT GAATGCTCGG 
CCAAGATCAC TATTCATGCC GGAAGACAAG AACTCTACAA AGTATAGAAT ATGGAGGCTG 
GTTACATCAC CGCCGTTTGA ATACTTTATC ATGACAATGA TTTGCTGCAA CACGCTCATT 
CTGATGATGA AGTACTACAA TAATCCATTG TTTTATGAAG AAATTTTGAG GCTTTTCAAT 
ACCGCATTAA CGGCCGTGTT TACAGTGGAG AGTATATTGA AGATTTTGGC ATTCGGTGTT 
AGAAATTACT TTCGTGATGG TTGGAATCGT TTCGATTTCG TCACCGTCGT CGGTTCTATC 
ACTGATGCGC TGGTCACAGA GTTCGGAGGT CATTTTGTGT CGCTTGGATT TCTTCGACTG 
TTCCGAGCAG CTCGTCTCAT TCGTCTCCTG CAGCAAGGAT ATACCATCCG AATCCTACTC 
TGGACGTTCG TTCAAAGTTT CAAAGCTCTA CCGTATGTGT GTCTTTTGAT TGGAATGCTC 
TTCTTCATTT ACGCAATTGT TGGAATGCAA GTATTCGGGA ATATCTGGTT GAATGCTGCC 
ACCGAAATCA ACAGACACAA CAATTTCCAG TCTTTCTTCA ACGCAGTTAT TTTACTCTTC 
CGATGCGCGA CTGGAGAAGG CTGGCAGGAT ATTATGATGG CTGCAGTTCA AGGGAAAGAT 
TGCGCACGAG CCGGCTCTGC TGAAATAAAC TTTGAAAAAG GCCAAACTTG CGGTAGCAAC 
GTTTCATATG CCTACTTCAC AAGTTTCGTT TTCCTCTCTT CTTTCTTGAT GCTCAATTTA 
TTCGTTGCCG TCATCATGGA TAATTTTGAC TACCTCACAA GAGATTCTTC CATTCTGGGT 
CCCCATCATT TAGACGAGTT TATCAGAGTC TGGGCAGATT ACGATCCAGC AGCAACGGGC 
CGAATTCACT ATTCCGAAAT GTATGAGATG TTAAGAATTA TGGCACCTCC TGTCGGTTTT 
GGAAAGAAAT GTCCATACCG ATTGGCTTAT AAACATCTAA TCCGAATGAA CATGCCGGTA 
GCAGAGGACG GAACTGTGCA CTTTACAACT ACCCTGTTCG CACTGATTCG AGAAAGTTTG 
AGCATAAAAA TGAGACCGGT GGAAGAGATG GATGAAGCTG ATGAGGAATT AAGATTGACA 
TTAAAGAAAA TCTGGCCACT GAAAGCAAAG AAAAACATGG TTGACTTGGT TGTTCCACCG 
AATCACGAAC TTTGCTTCCA AAAGCTCACA GTGGGAAAAA TCTACGCAGG ACTTTTGATT 
CTCGAAAACT ATCGAGCTCG AAAAAGCGGG ACGGAGGTCG GTGGACAAGG ATTGTTTGGC 
GGAGGCTTAC GATCGTTGGT CGCTGCAGCG AAAGCTGCCG AATCGCAACA TTCTTCCCAC 
ACTCCACAAC CGCCAGAGGA AACCACTCCG ATAATTCCAC AACATGCACA GCAGTTCTCG 
GCAGCACCGA CGATGAGCGC ACAAGGATCC TTGCAACAAA TGCAGGGAAC GAGTAGTGGC 
GGTGGTCAAC GTCCCTATTC ACTCTTCAAT AGCTTCGTCG ACACTATCAA ATCTGGAAAA 
CAGGATGGAG ACGTCACAGA TGTGCAATAT CAAAGTGTTG ATCAGCAGCA CGAAAAGATG 
AACTCGACTG GCAGGCGTTT GTCGGATATG TTCTCGAAAA TTCGACGAGG CACATCAGCC 
GACCACAATC CGCATCAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355464.1
CDS	1..6516
Translation	MNPLHRSSYRPTKTKYYLPLSRTPSHRKPKTSATSTYSAEKSGDESVTINIEFSYRSQSSGTSTLQKNRKPSEPPKSKRSSRDDNSNGYAGYEDATPRPSIDFPTAVIEAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368494.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368494.1

ORF Insert Sequence:

ATGAACCCAC TTCATCGGTC ATCATACAGA CCAACAAAGA CAAAGTATTA TCTACCATTA 
TCCAGAACTC CTTCCCATCG AAAACCAAAG ACGAGTGCAA CATCGACGTA TTCCGCAGAA 
AAATCAGGAG ATGAGTCTGT AACGATCAAC ATTGAATTTT CGTATCGATC GCAGTCTTCC 
GGGACGTCAA CCCTTCAAAA AAACAGGAAA CCTTCAGAAC CTCCAAAGTC AAAACGATCA 
AGCAGGGATG ATAATTCAAA TGGGTATGCA GGGTATGAAG ATGCAACGCC ACGACCCTCA 
ATCGACTTTC CAACTGCAGT TATTGAAGCT GCCCGTCGGG CGTCAGTTCT CGGTCGATCA 
GCTGCCGAAT CGTTATCTGC TCAAGAAGCA GCATCGAGCA GCGAACGGGG AGAGCACACT 
AACAGTCGCT CCCCATCGAC CAGCTATTCG AGTTGTATCG ACGACGAACG AAAGTTCTCG 
AGTCCTCATC GTAGACGAGT AGTCGATGTC AGCGATCATA AGACATGTGC ACTGTTGATG 
ACGAGGATGA AAGAGGCAAG TAGACAGCTG CCATCGCCGA GCCAACTAGC TGCAGAAGAG 
GCCCGAAGAG AGCAAAAAGC AGAGTCTGGC ACATTCGTCA GGAAGACAAC ACTTTCATCC 
AATGCACCTG TCAAAGAAAA GGGTCCGTCG TCCCTATTCA TCTTCGCCGA GGACAACATC 
ATTCGTCGAA ACGCGAAGGC CATCATAGAA TGGGGACCGT TCGAATACTT CATCTTATTG 
ACAATTATAG GCAACTGTGT GGTACTATCC ATGGAGCAAC ATCTGCCGAA AAACGACAAA 
AAGGCACTGT CGGAATGGCT GGAACGAACC GAACCATATT TTATGGGAAT TTTTTGTTTA 
GAATGTGTTT TGAAAGTCAT AGCGTTTGGT TTTGCATTAC ATAAAGGATC ATATCTTCGA 
TCAGGATGGA ATATCATGGA TTTTATTGTA GTAGTTTCCG GTGTCGTCAC CATGTTACCA 
TTCTCTCCGG CAACACAGAC CGCCAACCAA CCGGTGGATT CTGTCGATCT TCGAACATTG 
CGAGCTGTTC GAGTGCTTCG ACCGCTGAAA CTGGTTTCTG GAATTCCAAG TTTGCAAGTT 
GTCCTCAAGT CGATCTTGTG TGCTATGGCA CCATTGTTGC AAATTGGATT ACTTGTGTTG 
TTTGCCATTA TCATCTTCGC CATCATCGGA CTGGAATTTT ATTCGGGTGC ATTTCACAGC 
GCTTGCTACA ACGAACGCGG TGAAATTGAA AATGTTTCCG AGAGGCCAAT GCCGTGCACC 
AACAAAACGT CTCCAATGGG TGTTTATAAC TGTGATGTAA AAGGAACGAC TTGTTTGCAA 
AAATGGATAG GTCCTAATTA TGGAATTACT TCTTTCGACA ATATCGGATT TGCTATGATA 
ACTGTGTTTC AGTGCATTAC TATGGAAGGG TGGACCACTG TAATGTACTA CACAAACGAT 
TCACTTGGAA GCACATACAA CTGGGCTTAC TTTATTCCGC TCATTGTTCT AGGTTCTTTT 
TTCATGCTCA ATCTCGTTCT CGGAGTATTG TCTGGAGAGT TCGCCAAGGA AAGAGAACGT 
GTTGAAAATC GTCGGGAATT CTTGAAGCTT AGAAGGCAAC AACAAATTGA ACGAGAGCTT 
AACGGATATC TAGAATGGAT TTTAACAGCC GAAGAGGTGA TTCTAAAAGA AGATCGAACA 
ACTGAAGAAG AAAAAGCAGC GATCATGGAA GCCAGACGGC GAGCCGCTAA TAAAAAGTTA 
AAACAAGCCT CAAAACAGCA GTCTACAGAA ACTGAAGAGG ACTTTGAGGA GGACGAAGAC 
GAAATGGAAG AGGAATACGT CGACGAAGGT GGAACAGTAG AAGACGAATT CGCAGAACGT 
AAGAAACGAG GATGTTGCCA TTCAGTTGGA AAATTCATCA AACAACTGAG AATTCAAATC 
CGTATCATGG TGAAAACCCA AATCTTCTAC TGGTCTGTCA TCACATTGGT CTTTCTAAAC 
ACTTGCTGCG TTGCCAGTGA ACACTATGGC CAACCACAAT GGTTCACGGA CTTTCTGAAA 
TACGCCGAAT TCGTGTTCCT CGGAATTTTC GTAGTTGAAA TGCTTTTGAA ACTTTTCGCA 
ATGGGATCAA GAACATATTT TGCATCTAAA TTCAACCGTT TCGACTGTGT GGTCATCGTT 
GGATCAGCTG CTGAAGTAAT TTGGGCTGAA GTGTATGGAG GATCATTCGG TATTTCGGTG 
ATGAGAGCGC TGAGACTTCT TCGAATTTTC AAGCTCACAT CATACTGGGT GTCACTTCGT 
AATTTAGTCA GATCACTCAT GAACTCCATG AGGAGTATTA TTTCACTTCT CTTCCTACTT 
TTCTTGTTCA TTCTCATTTT CGCCCTTCTT GGAATGCAGT TGTTCGGAGG AAGATTCAAC 
TTTCCAACAA TGCACCCTTA CACACACTTT GACACTTTTC CAGTTGCTCT CATTACAGTA 
TTCCAAATCC TTACTGGAGA AGATTGGAAC GAAGTCATGT ATCTCGCGAT TGAATCACAA 
GGAGGTATCT ACAGTGGTGG ATGGCCATAC TCTATCTACT TTATTGTTTT AGTACTTTTC 
GGTAACTACA CTTTGTTGAA TGTATTCTTG GCTATCGCCG TCGACAACTT AGCCAACGCT 
CAGGAACTCA CTGCAGCTGA AGAAGCAGAC GAGAAAGCAA ATGAAATAGA GGAAGAATCT 
GAAGAACTTG ACGAACAGTA CCAAGAAGGT GATCATTGCA CCATTGATAT GGAGGGAAAA 
ACAGCTGGAG ACATGTGTGC GGTTGCAAGA GCTATGGATG ATTTGGACGA AGAATGCGAA 
GAAGAAGAGT CACCATTTGG AGGTCCAAAA CCCATGGTTC CCTACAGTTC TATGTTCTTT 
CTCTCGCCAA CTAATCCATT CCGAGTTCTG ATTCACAGTA TCGTGTGCAC CAAATACTTT 
GAAATGATGG TCATGACCGT TATCTGTCTC TCATCGGTTT CTCTAGCCGC TGAAGATCCA 
GTCGATGAAG AAAATCCGAG AAACAAAGTC CTACAGTATA TGGATTACTG TTTTACTGGA 
GTTTTTGCAT GTGAAATGCT TCTAAAGTTG ATTGATCAAG GTATTCTTCT ACATCCCGGA 
TCATATTGCC GCGATTTCTG GAATATTCTT GATGGAATTG TTGTAACATG CGCACTTTTC 
GCATTTGGTT TTGCAGGTAC CGAAGGAAGT GCTGGTAAAA ATTTGAACAC AATCAAATCG 
CTGAGGGTAC TTCGAGTGCT TCGTCCACTC AAAACAATCA AAAGAATTCC AAAGCTCAAG 
GCTGTATTCG ATTGTGTTGT TAATTCATTG AAGAATGTAT TCAACATTCT TATTGTTTAT 
TTTCTATTTC AATTCATTTT TGCTGTAATC GCAGTGCAGC TTTTCAACGG GAAATTTTTT 
TTCTGCACTG ACAAGAACCG AAAGTTCGCG AACACTTGCC ACGGGCAGTT CTTTGTCTAC 
GATAATCAGA ATGATCCACC ACGGGTTGAA CAACGTGAAT GGCGATTGAG ACCGTTCAAC 
TACGACAACA CAATCAACGC AATGCTCACA TTATTCGTTG TAACGACAGG AGAAGGATGG 
CCTGGCATTC GGCAGAACAG TATGGACACA ACGTTCGAAG ATCAGGGTCC AAGTCCATTT 
TTTCGGGTTG AAGTGGCTCT ATTCTATGTG ATGTTTTTCA TCGTCTTTCC ATTTTTCTTC 
GTCAACATTT TCGTCGCGTT GATTATCATC ACGTTTCAAG AACAAGGAGA AGCAGAGTTA 
AGCGAAGGAG ACTTGGACAA GAACCAAAAA CAATGTATCG ATTTTGCACT GAATGCTCGG 
CCAAGATCAC TATTCATGCC GGAAGACAAG AACTCTACAA AGTATAGAAT ATGGAGGCTG 
GTTACATCAC CGCCGTTTGA ATACTTTATC ATGACAATGA TTTGCTGCAA CACGCTCATT 
CTGATGATGA AGTACTACAA TAATCCATTG TTTTATGAAG AAATTTTGAG GCTTTTCAAT 
ACCGCATTAA CGGCCGTGTT TACAGTGGAG AGTATATTGA AGATTTTGGC ATTCGGTGTT 
AGAAATTACT TTCGTGATGG TTGGAATCGT TTCGATTTCG TCACCGTCGT CGGTTCTATC 
ACTGATGCGC TGGTCACAGA GTTCGGAGGT CATTTTGTGT CGCTTGGATT TCTTCGACTG 
TTCCGAGCAG CTCGTCTCAT TCGTCTCCTG CAGCAAGGAT ATACCATCCG AATCCTACTC 
TGGACGTTCG TTCAAAGTTT CAAAGCTCTA CCGTATGTGT GTCTTTTGAT TGGAATGCTC 
TTCTTCATTT ACGCAATTGT TGGAATGCAA GTATTCGGGA ATATCTGGTT GAATGCTGCC 
ACCGAAATCA ACAGACACAA CAATTTCCAG TCTTTCTTCA ACGCAGTTAT TTTACTCTTC 
CGATGCGCGA CTGGAGAAGG CTGGCAGGAT ATTATGATGG CTGCAGTTCA AGGGAAAGAT 
TGCGCACGAG CCGGCTCTGC TGAAATAAAC TTTGAAAAAG GCCAAACTTG CGGTAGCAAC 
GTTTCATATG CCTACTTCAC AAGTTTCGTT TTCCTCTCTT CTTTCTTGAT GCTCAATTTA 
TTCGTTGCCG TCATCATGGA TAATTTTGAC TACCTCACAA GAGATTCTTC CATTCTGGGT 
CCCCATCATT TAGACGAGTT TATCAGAGTC TGGGCAGATT ACGATCCAGC AGCAACGGGC 
CGAATTCACT ATTCCGAAAT GTATGAGATG TTAAGAATTA TGGCACCTCC TGTCGGTTTT 
GGAAAGAAAT GTCCATACCG ATTGGCTTAT AAACATCTAA TCCGAATGAA CATGCCGGTA 
GCAGAGGACG GAACTGTGCA CTTTACAACT ACCCTGTTCG CACTGATTCG AGAAAGTTTG 
AGCATAAAAA TGAGACCGGT GGAAGAGATG GATGAAGCTG ATGAGGAATT AAGATTGACA 
TTAAAGAAAA TCTGGCCACT GAAAGCAAAG AAAAACATGG TTGACTTGGT TGTTCCACCG 
AATCACGAAC TTTGCTTCCA AAAGCTCACA GTGGGAAAAA TCTACGCAGG ACTTTTGATT 
CTCGAAAACT ATCGAGCTCG AAAAAGCGGG ACGGAGGTCG GTGGACAAGG ATTGTTTGGC 
GGAGGCTTAC GATCGTTGGT CGCTGCAGCG AAAGCTGCCG AATCGCAACA TTCTTCCCAC 
ACTCCACAAC CGCCAGAGGA AACCACTCCG ATAATTCCAC AACATGCACA GCAGTTCTCG 
GCAGCACCGA CGATGAGCGC ACAAGGATCC TTGCAACAAA TGCAGGGAAC GAGTAGTGGC 
GGTGGTCAAC GTCCCTATTC ACTCTTCAAT AGCTTCGTCG ACACTATCAA ATCTGGAAAA 
CAGGATGGAG ACGTCACAGA TGTGCAATAT CAAAGTGTTG ATCAGCAGCA CGAAAAGATG 
AACTCGACTG GCAGGCGTTT GTCGGATATG TTCTCGAAAA TTCGACGAGG CACATCAGCC 
GACCACAATC CGCATCAGAC CGAACATTTG TTGGCTCAAG ATAATCGATC TCCATCGTCA 
CCTAGATACC GAAGCATGGC CCGCGCGTCA CCGCCGAGTC CTGCTGAACG ATACGGTCAT 
CCACCCAGAT ATCGCACCGA ATCGCCTCCA TCTTCGAGAT CGGAGTACCA GATGTCAATT 
AGAGACCCGA TAATTAGGCG TAATCGCTAT AATACAATGG AACACTCGCG ATCTTCCCAC 
GATCCACAAT ATCATCAGGA TCAACAACAA CAACAACAAC CACATCACCA GCAACACTCA 
CAACATTTGC AACATTCACA CCACAAAACG TACCAAAATC ATAATCAATA TTCAAGAAGT 
CCAATCTATT CCGATGATTC GAGTGTTGCT GAAAGTTATC GAAGAGAGCG AGAATTCCGA 
AGATATCAAG ACTCCACTCC ACAAGATGTC AGCGAGGACG ACGACCCGAT GCCTACGGCG 
GTCCGAGCGC GCCGATTACC ACTGATTTCC ACAATGCCCA CGCACTACGA GTCGGCCTAC 
CAGCCATCAT CATATAACCA GCATCTCAAT GATTCATATG GTTTAGGAAC CGGGTACCAA 
CGAGATTATC ACACTAGTCA TTCACACTCG CACCATCCCA CCTCACAACA ACAACAACAT 
CAGCCCATGT ACAGCACGTC ACCACTAATA TCACCGAGAT CGAGTCATAG CTACTACACG 
CCGAGGAGCT CGCAATACTA TGAAATACCA TCGCCGAGTC CAGATATTTA TCCATCGTAT 
CGTGGATCGG CATCACCTCG CAGGTACCCC ACCAGTACCG TCGTAGTGGC TCCAGATCGA 
GAAGGTTCGA GCGCTCGCGT CATTCAAGCT CAGCCTGGCT CGATACCACT ATCTGACTCG 
GAAACAGAAG ATGATCCTCG ATGGGCAATT GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368495.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355463.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa698994
Clone ID Related Accession (Same CDS sequence)	NM_001368495.3 , NM_001368495.1
Accession Version	NM_001368495.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368495.2. COMPLETENESS: incomplete on the 5' end.

ATGTTTGTGA CTATTACAAG TACCACTATT CCATTCAGTT CTTCAACAGT TTCATTTCAA 
GAACCTCCAA AGTCAAAACG ATCAAGCAGG GATGATAATT CAAATGGGTA TGCAGGGTAT 
GAAGATGCAA CGCCACGACC CTCAATCGAC TTTCCAACTG CAGTTATTGA AGCTGCCCGT 
CGGGCGTCAG TTCTCGGTCG ATCAGCTGCC GAATCGTTAT CTGCTCAAGA AGCAGCATCG 
AGCAGCGAAC GGGGAGAGCA CACTAACAGT CGCTCCCCAT CGACCAGCTA TTCGAGTTGT 
ATCGACGACG AACGAAAGTT CTCGAGTCCT CATCGTAGAC GAGTAGTCGA TGTCAGCGAT 
CATAAGACAT GTGCACTGTT GATGACGAGG ATGAAAGAGG CAAGTAGACA GCTGCCATCG 
CCGAGCCAAC TAGCTGCAGA AGAGGCCCGA AGAGAGCAAA AAGCAGAGTC TGGCACATTC 
GTCAGGAAGA CAACACTTTC ATCCAATGCA CCTGTCAAAG AAAAGGGTCC GTCGTCCCTA 
TTCATCTTCG CCGAGGACAA CATCATTCGT CGAAACGCGA AGGCCATCAT AGAATGGGGA 
CCGTTCGAAT ACTTCATCTT ATTGACAATT ATAGGCAACT GTGTGGTACT ATCCATGGAG 
CAACATCTGC CGAAAAACGA CAAAAAGGCA CTGTCGGAAT GGCTGGAACG AACCGAACCA 
TATTTTATGG GAATTTTTTG TTTAGAATGT GTTTTGAAAG TCATAGCGTT TGGTTTTGCA 
TTACATAAAG GATCATATCT TCGATCAGGA TGGAATATCA TGGATTTTAT TGTAGTAGTT 
TCCGGTGTCG TCACCATGTT ACCATTCTCT CCGGCAACAC AGACCGCCAA CCAACCGGTG 
GATTCTGTCG ATCTTCGAAC ATTGCGAGCT GTTCGAGTGC TTCGACCGCT GAAACTGGTT 
TCTGGAATTC CAAGTTTGCA AGTTGTCCTC AAGTCGATCT TGTGTGCTAT GGCACCATTG 
TTGCAAATTG GATTACTTGT GTTGTTTGCC ATTATCATCT TCGCCATCAT CGGACTGGAA 
TTTTATTCGG GTGCATTTCA CAGCGCTTGC TACAACGAAC GCGGTGAAAT TGAAAATGTT 
TCCGAGAGGC CAATGCCGTG CACCAACAAA ACGTCTCCAA TGGGTGTTTA TAACTGTGAT 
GTAAAAGGAA CGACTTGTTT GCAAAAATGG ATAGGTCCTA ATTATGGAAT TACTTCTTTC 
GACAATATCG GATTTGCTAT GATAACTGTG TTTCAGTGCA TTACTATGGA AGGGTGGACC 
ACTGTAATGT ACTACACAAA CGATTCACTT GGAAGCACAT ACAACTGGGC TTACTTTATT 
CCGCTCATTG TTCTAGGTTC TTTTTTCATG CTCAATCTCG TTCTCGGAGT ATTGTCTGGA 
GAGTTCGCCA AGGAAAGAGA ACGTGTTGAA AATCGTCGGG AATTCTTGAA GCTTAGAAGG 
CAACAACAAA TTGAACGAGA GCTTAACGGA TATCTAGAAT GGATTTTAAC AGCCGAAGAG 
GTGATTCTAA AAGAAGATCG AACAACTGAA GAAGAAAAAG CAGCGATCAT GGAAGCCAGA 
CGGCGAGCCG CTAATAAAAA GTTAAAACAA GCCTCAAAAC AGCAGTCTAC AGAAACTGAA 
GAGGACTTTG AGGAGGACGA AGACGAAATG GAAGAGGAAT ACGTCGACGA AGGTGGAACA 
GTAGAAGACG AATTCGCAGA ACGTAAGAAA CGAGGATGTT GCCATTCAGT TGGAAAATTC 
ATCAAACAAC TGAGAATTCA AATCCGTATC ATGGTGAAAA CCCAAATCTT CTACTGGTCT 
GTCATCACAT TGGTCTTTCT AAACACTTGC TGCGTTGCCA GTGAACACTA TGGCCAACCA 
CAATGGTTCA CGGACTTTCT GAAATACGCC GAATTCGTGT TCCTCGGAAT TTTCGTAGTT 
GAAATGCTTT TGAAACTTTT CGCAATGGGA TCAAGAACAT ATTTTGCATC TAAATTCAAC 
CGTTTCGACT GTGTGGTCAT CGTTGGATCA GCTGCTGAAG TAATTTGGGC TGAAGTGTAT 
GGAGGATCAT TCGGTATTTC GGTGATGAGA GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC 
ACATCATACT GGGTGTCACT TCGTAATTTA GTCAGATCAC TCATGAACTC CATGAGGAGT 
ATTATTTCAC TTCTCTTCCT ACTTTTCTTG TTCATTCTCA TTTTCGCCCT TCTTGGAATG 
CAGTTGTTCG GAGGAAGATT CAACTTTCCA ACAATGCACC CTTACACACA CTTTGACACT 
TTTCCAGTTG CTCTCATTAC AGTATTCCAA ATCCTTACTG GAGAAGATTG GAACGAAGTC 
ATGTATCTCG CGATTGAATC ACAAGGAGGT ATCTACAGTG GTGGATGGCC ATACTCTATC 
TACTTTATTG TTTTAGTACT TTTCGGTAAC TACACTTTGT TGAATGTATT CTTGGCTATC 
GCCGTCGACA ACTTAGCCAA CGCTCAGGAA CTCACTGCAG CTGAAGAAGC AGACGAGAAA 
GCAAATGAAA TAGAGGAAGA ATCTGAAGAA CTTGACGAAC AGTACCAAGA AGGTGATCAT 
TGCACCATTG ATATGGAGGG AAAAACAGCT GGAGACATGT GTGCGGTTGC AAGAGCTATG 
GATGATTTGG ACGAAGAATG CGAAGAAGAA GAGTCACCAT TTGGAGGTCC AAAACCCATG 
GTTCCCTACA GTTCTATGTT CTTTCTCTCG CCAACTAATC CATTCCGAGT TCTGATTCAC 
AGTATCGTGT GCACCAAATA CTTTGAAATG ATGGTCATGA CCGTTATCTG TCTCTCATCG 
GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT GAAGAAAATC CGAGAAACAA AGTCCTACAG 
TATATGGATT ACTGTTTTAC TGGAGTTTTT GCATGTGAAA TGCTTCTAAA GTTGATTGAT 
CAAGGTATTC TTCTACATCC CGGATCATAT TGCCGCGATT TCTGGAATAT TCTTGATGGA 
ATTGTTGTAA CATGCGCACT TTTCGCATTT GGTTTTGCAG GTACCGAAGG AAGTGCTGGT 
AAAAATTTGA ACACAATCAA ATCGCTGAGG GTACTTCGAG TGCTTCGTCC ACTCAAAACA 
ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA TTCGATTGTG TTGTTAATTC ATTGAAGAAT 
GTATTCAACA TTCTTATTGT TTATTTTCTA TTTCAATTCA TTTTTGCTGT AATCGCAGTG 
CAGCTTTTCA ACGGGAAATT TTTTTTCTGC ACTGACAAGA ACCGAAAGTT CGCGAACACT 
TGCCACGGGC AGTTCTTTGT CTACGATAAT CAGAATGATC CACCACGGGT TGAACAACGT 
GAATGGCGAT TGAGACCGTT CAACTACGAC AACACAATCA ACGCAATGCT CACATTATTC 
GTTGTAACGA CAGGAGAAGG ATGGCCTGGC ATTCGGCAGA ACAGTATGGA CACAACGTTC 
GAAGATCAGG GTCCAAGTCC ATTTTTTCGG GTTGAAGTGG CTCTATTCTA TGTGATGTTT 
TTCATCGTCT TTCCATTTTT CTTCGTCAAC ATTTTCGTCG CGTTGATTAT CATCACGTTT 
CAAGAACAAG GAGAAGCAGA GTTAAGCGAA GGAGACTTGG ACAAGAACCA AAAACAATGT 
ATCGATTTTG CACTGAATGC TCGGCCAAGA TCACTATTCA TGCCGGAAGA CAAGAACTCT 
ACAAAGTATA GAATATGGAG GCTGGTTACA TCACCGCCGT TTGAATACTT TATCATGACA 
ATGATTTGCT GCAACACGCT CATTCTGATG ATGAAGTACT ACAATAATCC ATTGTTTTAT 
GAAGAAATTT TGAGGCTTTT CAATACCGCA TTAACGGCCG TGTTTACAGT GGAGAGTATA 
TTGAAGATTT TGGCATTCGG TGTTAGAAAT TACTTTCGTG ATGGTTGGAA TCGTTTCGAT 
TTCGTCACCG TCGTCGGTTC TATCACTGAT GCGCTGGTCA CAGAGTTCGG AGGTCATTTT 
GTGTCGCTTG GATTTCTTCG ACTGTTCCGA GCAGCTCGTC TCATTCGTCT CCTGCAGCAA 
GGATATACCA TCCGAATCCT ACTCTGGACG TTCGTTCAAA GTTTCAAAGC TCTACCGTAT 
GTGTGTCTTT TGATTGGAAT GCTCTTCTTC ATTTACGCAA TTGTTGGAAT GCAAGTATTC 
GGGAATATCT GGTTGAATGC TGCCACCGAA ATCAACAGAC ACAACAATTT CCAGTCTTTC 
TTCAACGCAG TTATTTTACT CTTCCGATGC GCGACTGGAG AAGGCTGGCA GGATATTATG 
ATGGCTGCAG TTCAAGGGAA AGATTGCGCA CGAGCCGGCT CTGCTGAAAT AAACTTTGAA 
AAAGGCCAAA CTTGCGGTAG CAACGTTTCA TATGCCTACT TCACAAGTTT CGTTTTCCTC 
TCTTCTTTCT TGATGCTCAA TTTATTCGTT GCCGTCATCA TGGATAATTT TGACTACCTC 
ACAAGAGATT CTTCCATTCT GGGTCCCCAT CATTTAGACG AGTTTATCAG AGTCTGGGCA 
GATTACGATC CAGCAGCAAC GGGCCGAATT CACTATTCCG AAATGTATGA GATGTTAAGA 
ATTATGGCAC CTCCTGTCGG TTTTGGAAAG AAATGTCCAT ACCGATTGGC TTATAAACAT 
CTAATCCGAA TGAACATGCC GGTAGCAGAG GACGGAACTG TGCACTTTAC AACTACCCTG 
TTCGCACTGA TTCGAGAAAG TTTGAGCATA AAAATGAGAC CGGTGGAAGA GATGGATGAA 
GCTGATGAGG AATTAAGATT GACATTAAAG AAAATCTGGC CACTGAAAGC AAAGAAAAAC 
ATGGTTGACT TGGTTGTTCC ACCGAATCAC GAACTTTGCT TCCAAAAGCT CACAGTGGGA 
AAAATCTACG CAGGACTTTT GATTCTCGAA AACTATCGAG CTCGAAAAAG CGGGACGGAG 
GTCGGTGGAC AAGGATTGTT TGGCGGAGGC TTACGATCGT TGGTCGCTGC AGCGAAAGCT 
GCCGAATCGC AACATTCTTC CCACACTCCA CAACCGCCAG AGGAAACCAC TCCGATAATT 
CCACAACATG CACAGCAGTT CTCGGCAGCA CCGACGATGA GCGCACAAGG ATCCTTGCAA 
CAAATGCAGG GAACGAGTAG TGGCGGTGGT CAACGTCCCT ATTCACTCTT CAATAGCTTC 
GTCGACACTA TCAAATCTGG AAAACAGGAT GGAGACGTCA CAGATGTGCA ATATCAAAGT 
GTTGATCAGC AGCACGAAAA GATGAACTCG ACTGGCAGGC GTTTGTCGGA TATGTTCTCG 
AAAATTCGAC GAGGCACATC AGCCGACCAC AATCCGCATC AGACCGAACA TTTGTTGGCT 
CAAGATAATC GATCTCCATC GTCACCTAGA TACCGAAGCA TGGCCCGCGC GTCACCGCCG 
AGTCCTGCTG AACGATACGG TCATCCACCC AGATATCGCA CCGAATCGCC TCCATCTTCG 
AGATCGGAGT ACCAGATGTC AATTAGAGAC CCGATAATTA GGCGTAATCG CTATAATACA 
ATGGAACACT CGCGATCTTC CCACGATCCA CAATATCATC AGGATCAACA ACAACAACAA 
CAACCACATC ACCAGCAACA CTCACAACAT TTGCAACATT CACACCACAA AACGTACCAA 
AATCATAATC AATATTCAAG AAGTCCAATC TATTCCGATG ATTCGAGTGT TGCTGAAAGT 
TATCGAAGAG AGCGAGAATT CCGAAGATAT CAAGACTCCA CTCCACAAGA TGTCAGCGAG 
GACGACGACC CGATGCCTAC GGCGGTCCGA GCGCGCCGAT TACCACTGAT TTCCACAATG 
CCCACGCACT ACGAGTCGGC CTACCAGCCA TCATCATATA ACCAGCATCT CAATGATTCA 
TATGGTTTAG GAACCGGGTA CCAACGAGAT TATCACACTA GTCATTCACA CTCGCACCAT 
CCCACCTCAC AACAACAACA ACATCAGCCC ATGTACAGCA CGTCACCACT AATATCACCG 
AGATCGAGTC ATAGCTACTA CACGCCGAGG AGCTCGCAAT ACTATGAAAT ACCATCGCCG 
AGTCCAGATA TTTATCCATC GTATCGTGGA TCGGCATCAC CTCGCAGGTA CCCCACCAGT 
ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT TCGAGCGCTC GCGTCATTCA AGCTCAGCCT 
GGCTCGATAC CACTATCTGA CTCGGAAACA GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355463.1
CDS	1..6360
Translation	MFVTITSTTIPFSSSTVSFQEPPKSKRSSRDDNSNGYAGYEDATPRPSIDFPTAVIEAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368495.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368495.3

ORF Insert Sequence:

ATGTTTGTGA CTATTACAAG TACCACTATT CCATTCAGTT CTTCAACAGT TTCATTTCAA 
GAACCTCCAA AGTCAAAACG ATCAAGCAGG GATGATAATT CAAATGGGTA TGCAGGGTAT 
GAAGATGCAA CGCCACGACC CTCAATCGAC TTTCCAACTG CAGTTATTGA AGCTGCCCGT 
CGGGCGTCAG TTCTCGGTCG ATCAGCTGCC GAATCGTTAT CTGCTCAAGA AGCAGCATCG 
AGCAGCGAAC GGGGAGAGCA CACTAACAGT CGCTCCCCAT CGACCAGCTA TTCGAGTTGT 
ATCGACGACG AACGAAAGTT CTCGAGTCCT CATCGTAGAC GAGTAGTCGA TGTCAGCGAT 
CATAAGACAT GTGCACTGTT GATGACGAGG ATGAAAGAGG CAAGTAGACA GCTGCCATCG 
CCGAGCCAAC TAGCTGCAGA AGAGGCCCGA AGAGAGCAAA AAGCAGAGTC TGGCACATTC 
GTCAGGAAGA CAACACTTTC ATCCAATGCA CCTGTCAAAG AAAAGGGTCC GTCGTCCCTA 
TTCATCTTCG CCGAGGACAA CATCATTCGT CGAAACGCGA AGGCCATCAT AGAATGGGGA 
CCGTTCGAAT ACTTCATCTT ATTGACAATT ATAGGCAACT GTGTGGTACT ATCCATGGAG 
CAACATCTGC CGAAAAACGA CAAAAAGGCA CTGTCGGAAT GGCTGGAACG AACCGAACCA 
TATTTTATGG GAATTTTTTG TTTAGAATGT GTTTTGAAAG TCATAGCGTT TGGTTTTGCA 
TTACATAAAG GATCATATCT TCGATCAGGA TGGAATATCA TGGATTTTAT TGTAGTAGTT 
TCCGGTGTCG TCACCATGTT ACCATTCTCT CCGGCAACAC AGACCGCCAA CCAACCGGTG 
GATTCTGTCG ATCTTCGAAC ATTGCGAGCT GTTCGAGTGC TTCGACCGCT GAAACTGGTT 
TCTGGAATTC CAAGTTTGCA AGTTGTCCTC AAGTCGATCT TGTGTGCTAT GGCACCATTG 
TTGCAAATTG GATTACTTGT GTTGTTTGCC ATTATCATCT TCGCCATCAT CGGACTGGAA 
TTTTATTCGG GTGCATTTCA CAGCGCTTGC TACAACGAAC GCGGTGAAAT TGAAAATGTT 
TCCGAGAGGC CAATGCCGTG CACCAACAAA ACGTCTCCAA TGGGTGTTTA TAACTGTGAT 
GTAAAAGGAA CGACTTGTTT GCAAAAATGG ATAGGTCCTA ATTATGGAAT TACTTCTTTC 
GACAATATCG GATTTGCTAT GATAACTGTG TTTCAGTGCA TTACTATGGA AGGGTGGACC 
ACTGTAATGT ACTACACAAA CGATTCACTT GGAAGCACAT ACAACTGGGC TTACTTTATT 
CCGCTCATTG TTCTAGGTTC TTTTTTCATG CTCAATCTCG TTCTCGGAGT ATTGTCTGGA 
GAGTTCGCCA AGGAAAGAGA ACGTGTTGAA AATCGTCGGG AATTCTTGAA GCTTAGAAGG 
CAACAACAAA TTGAACGAGA GCTTAACGGA TATCTAGAAT GGATTTTAAC AGCCGAAGAG 
GTGATTCTAA AAGAAGATCG AACAACTGAA GAAGAAAAAG CAGCGATCAT GGAAGCCAGA 
CGGCGAGCCG CTAATAAAAA GTTAAAACAA GCCTCAAAAC AGCAGTCTAC AGAAACTGAA 
GAGGACTTTG AGGAGGACGA AGACGAAATG GAAGAGGAAT ACGTCGACGA AGGTGGAACA 
GTAGAAGACG AATTCGCAGA ACGTAAGAAA CGAGGATGTT GCCATTCAGT TGGAAAATTC 
ATCAAACAAC TGAGAATTCA AATCCGTATC ATGGTGAAAA CCCAAATCTT CTACTGGTCT 
GTCATCACAT TGGTCTTTCT AAACACTTGC TGCGTTGCCA GTGAACACTA TGGCCAACCA 
CAATGGTTCA CGGACTTTCT GAAATACGCC GAATTCGTGT TCCTCGGAAT TTTCGTAGTT 
GAAATGCTTT TGAAACTTTT CGCAATGGGA TCAAGAACAT ATTTTGCATC TAAATTCAAC 
CGTTTCGACT GTGTGGTCAT CGTTGGATCA GCTGCTGAAG TAATTTGGGC TGAAGTGTAT 
GGAGGATCAT TCGGTATTTC GGTGATGAGA GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC 
ACATCATACT GGGTGTCACT TCGTAATTTA GTCAGATCAC TCATGAACTC CATGAGGAGT 
ATTATTTCAC TTCTCTTCCT ACTTTTCTTG TTCATTCTCA TTTTCGCCCT TCTTGGAATG 
CAGTTGTTCG GAGGAAGATT CAACTTTCCA ACAATGCACC CTTACACACA CTTTGACACT 
TTTCCAGTTG CTCTCATTAC AGTATTCCAA ATCCTTACTG GAGAAGATTG GAACGAAGTC 
ATGTATCTCG CGATTGAATC ACAAGGAGGT ATCTACAGTG GTGGATGGCC ATACTCTATC 
TACTTTATTG TTTTAGTACT TTTCGGTAAC TACACTTTGT TGAATGTATT CTTGGCTATC 
GCCGTCGACA ACTTAGCCAA CGCTCAGGAA CTCACTGCAG CTGAAGAAGC AGACGAGAAA 
GCAAATGAAA TAGAGGAAGA ATCTGAAGAA CTTGACGAAC AGTACCAAGA AGGTGATCAT 
TGCACCATTG ATATGGAGGG AAAAACAGCT GGAGACATGT GTGCGGTTGC AAGAGCTATG 
GATGATTTGG ACGAAGAATG CGAAGAAGAA GAGTCACCAT TTGGAGGTCC AAAACCCATG 
GTTCCCTACA GTTCTATGTT CTTTCTCTCG CCAACTAATC CATTCCGAGT TCTGATTCAC 
AGTATCGTGT GCACCAAATA CTTTGAAATG ATGGTCATGA CCGTTATCTG TCTCTCATCG 
GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT GAAGAAAATC CGAGAAACAA AGTCCTACAG 
TATATGGATT ACTGTTTTAC TGGAGTTTTT GCATGTGAAA TGCTTCTAAA GTTGATTGAT 
CAAGGTATTC TTCTACATCC CGGATCATAT TGCCGCGATT TCTGGAATAT TCTTGATGGA 
ATTGTTGTAA CATGCGCACT TTTCGCATTT GGTTTTGCAG GTACCGAAGG AAGTGCTGGT 
AAAAATTTGA ACACAATCAA ATCGCTGAGG GTACTTCGAG TGCTTCGTCC ACTCAAAACA 
ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA TTCGATTGTG TTGTTAATTC ATTGAAGAAT 
GTATTCAACA TTCTTATTGT TTATTTTCTA TTTCAATTCA TTTTTGCTGT AATCGCAGTG 
CAGCTTTTCA ACGGGAAATT TTTTTTCTGC ACTGACAAGA ACCGAAAGTT CGCGAACACT 
TGCCACGGGC AGTTCTTTGT CTACGATAAT CAGAATGATC CACCACGGGT TGAACAACGT 
GAATGGCGAT TGAGACCGTT CAACTACGAC AACACAATCA ACGCAATGCT CACATTATTC 
GTTGTAACGA CAGGAGAAGG ATGGCCTGGC ATTCGGCAGA ACAGTATGGA CACAACGTTC 
GAAGATCAGG GTCCAAGTCC ATTTTTTCGG GTTGAAGTGG CTCTATTCTA TGTGATGTTT 
TTCATCGTCT TTCCATTTTT CTTCGTCAAC ATTTTCGTCG CGTTGATTAT CATCACGTTT 
CAAGAACAAG GAGAAGCAGA GTTAAGCGAA GGAGACTTGG ACAAGAACCA AAAACAATGT 
ATCGATTTTG CACTGAATGC TCGGCCAAGA TCACTATTCA TGCCGGAAGA CAAGAACTCT 
ACAAAGTATA GAATATGGAG GCTGGTTACA TCACCGCCGT TTGAATACTT TATCATGACA 
ATGATTTGCT GCAACACGCT CATTCTGATG ATGAAGTACT ACAATAATCC ATTGTTTTAT 
GAAGAAATTT TGAGGCTTTT CAATACCGCA TTAACGGCCG TGTTTACAGT GGAGAGTATA 
TTGAAGATTT TGGCATTCGG TGTTAGAAAT TACTTTCGTG ATGGTTGGAA TCGTTTCGAT 
TTCGTCACCG TCGTCGGTTC TATCACTGAT GCGCTGGTCA CAGAGTTCGG AGGTCATTTT 
GTGTCGCTTG GATTTCTTCG ACTGTTCCGA GCAGCTCGTC TCATTCGTCT CCTGCAGCAA 
GGATATACCA TCCGAATCCT ACTCTGGACG TTCGTTCAAA GTTTCAAAGC TCTACCGTAT 
GTGTGTCTTT TGATTGGAAT GCTCTTCTTC ATTTACGCAA TTGTTGGAAT GCAAGTATTC 
GGGAATATCT GGTTGAATGC TGCCACCGAA ATCAACAGAC ACAACAATTT CCAGTCTTTC 
TTCAACGCAG TTATTTTACT CTTCCGATGC GCGACTGGAG AAGGCTGGCA GGATATTATG 
ATGGCTGCAG TTCAAGGGAA AGATTGCGCA CGAGCCGGCT CTGCTGAAAT AAACTTTGAA 
AAAGGCCAAA CTTGCGGTAG CAACGTTTCA TATGCCTACT TCACAAGTTT CGTTTTCCTC 
TCTTCTTTCT TGATGCTCAA TTTATTCGTT GCCGTCATCA TGGATAATTT TGACTACCTC 
ACAAGAGATT CTTCCATTCT GGGTCCCCAT CATTTAGACG AGTTTATCAG AGTCTGGGCA 
GATTACGATC CAGCAGCAAC GGGCCGAATT CACTATTCCG AAATGTATGA GATGTTAAGA 
ATTATGGCAC CTCCTGTCGG TTTTGGAAAG AAATGTCCAT ACCGATTGGC TTATAAACAT 
CTAATCCGAA TGAACATGCC GGTAGCAGAG GACGGAACTG TGCACTTTAC AACTACCCTG 
TTCGCACTGA TTCGAGAAAG TTTGAGCATA AAAATGAGAC CGGTGGAAGA GATGGATGAA 
GCTGATGAGG AATTAAGATT GACATTAAAG AAAATCTGGC CACTGAAAGC AAAGAAAAAC 
ATGGTTGACT TGGTTGTTCC ACCGAATCAC GAACTTTGCT TCCAAAAGCT CACAGTGGGA 
AAAATCTACG CAGGACTTTT GATTCTCGAA AACTATCGAG CTCGAAAAAG CGGGACGGAG 
GTCGGTGGAC AAGGATTGTT TGGCGGAGGC TTACGATCGT TGGTCGCTGC AGCGAAAGCT 
GCCGAATCGC AACATTCTTC CCACACTCCA CAACCGCCAG AGGAAACCAC TCCGATAATT 
CCACAACATG CACAGCAGTT CTCGGCAGCA CCGACGATGA GCGCACAAGG ATCCTTGCAA 
CAAATGCAGG GAACGAGTAG TGGCGGTGGT CAACGTCCCT ATTCACTCTT CAATAGCTTC 
GTCGACACTA TCAAATCTGG AAAACAGGAT GGAGACGTCA CAGATGTGCA ATATCAAAGT 
GTTGATCAGC AGCACGAAAA GATGAACTCG ACTGGCAGGC GTTTGTCGGA TATGTTCTCG 
AAAATTCGAC GAGGCACATC AGCCGACCAC AATCCGCATC AGACCGAACA TTTGTTGGCT 
CAAGATAATC GATCTCCATC GTCACCTAGA TACCGAAGCA TGGCCCGCGC GTCACCGCCG 
AGTCCTGCTG AACGATACGG TCATCCACCC AGATATCGCA CCGAATCGCC TCCATCTTCG 
AGATCGGAGT ACCAGATGTC AATTAGAGAC CCGATAATTA GGCGTAATCG CTATAATACA 
ATGGAACACT CGCGATCTTC CCACGATCCA CAATATCATC AGGATCAACA ACAACAACAA 
CAACCACATC ACCAGCAACA CTCACAACAT TTGCAACATT CACACCACAA AACGTACCAA 
AATCATAATC AATATTCAAG AAGTCCAATC TATTCCGATG ATTCGAGTGT TGCTGAAAGT 
TATCGAAGAG AGCGAGAATT CCGAAGATAT CAAGACTCCA CTCCACAAGA TGTCAGCGAG 
GACGACGACC CGATGCCTAC GGCGGTCCGA GCGCGCCGAT TACCACTGAT TTCCACAATG 
CCCACGCACT ACGAGTCGGC CTACCAGCCA TCATCATATA ACCAGCATCT CAATGATTCA 
TATGGTTTAG GAACCGGGTA CCAACGAGAT TATCACACTA GTCATTCACA CTCGCACCAT 
CCCACCTCAC AACAACAACA ACATCAGCCC ATGTACAGCA CGTCACCACT AATATCACCG 
AGATCGAGTC ATAGCTACTA CACGCCGAGG AGCTCGCAAT ACTATGAAAT ACCATCGCCG 
AGTCCAGATA TTTATCCATC GTATCGTGGA TCGGCATCAC CTCGCAGGTA CCCCACCAGT 
ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT TCGAGCGCTC GCGTCATTCA AGCTCAGCCT 
GGCTCGATAC CACTATCTGA CTCGGAAACA GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368495.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355463.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa698994
Clone ID Related Accession (Same CDS sequence)	NM_001368495.3 , NM_001368495.1
Accession Version	NM_001368495.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGTTTGTGA CTATTACAAG TACCACTATT CCATTCAGTT CTTCAACAGT TTCATTTCAA 
GAACCTCCAA AGTCAAAACG ATCAAGCAGG GATGATAATT CAAATGGGTA TGCAGGGTAT 
GAAGATGCAA CGCCACGACC CTCAATCGAC TTTCCAACTG CAGTTATTGA AGCTGCCCGT 
CGGGCGTCAG TTCTCGGTCG ATCAGCTGCC GAATCGTTAT CTGCTCAAGA AGCAGCATCG 
AGCAGCGAAC GGGGAGAGCA CACTAACAGT CGCTCCCCAT CGACCAGCTA TTCGAGTTGT 
ATCGACGACG AACGAAAGTT CTCGAGTCCT CATCGTAGAC GAGTAGTCGA TGTCAGCGAT 
CATAAGACAT GTGCACTGTT GATGACGAGG ATGAAAGAGG CAAGTAGACA GCTGCCATCG 
CCGAGCCAAC TAGCTGCAGA AGAGGCCCGA AGAGAGCAAA AAGCAGAGTC TGGCACATTC 
GTCAGGAAGA CAACACTTTC ATCCAATGCA CCTGTCAAAG AAAAGGGTCC GTCGTCCCTA 
TTCATCTTCG CCGAGGACAA CATCATTCGT CGAAACGCGA AGGCCATCAT AGAATGGGGA 
CCGTTCGAAT ACTTCATCTT ATTGACAATT ATAGGCAACT GTGTGGTACT ATCCATGGAG 
CAACATCTGC CGAAAAACGA CAAAAAGGCA CTGTCGGAAT GGCTGGAACG AACCGAACCA 
TATTTTATGG GAATTTTTTG TTTAGAATGT GTTTTGAAAG TCATAGCGTT TGGTTTTGCA 
TTACATAAAG GATCATATCT TCGATCAGGA TGGAATATCA TGGATTTTAT TGTAGTAGTT 
TCCGGTGTCG TCACCATGTT ACCATTCTCT CCGGCAACAC AGACCGCCAA CCAACCGGTG 
GATTCTGTCG ATCTTCGAAC ATTGCGAGCT GTTCGAGTGC TTCGACCGCT GAAACTGGTT 
TCTGGAATTC CAAGTTTGCA AGTTGTCCTC AAGTCGATCT TGTGTGCTAT GGCACCATTG 
TTGCAAATTG GATTACTTGT GTTGTTTGCC ATTATCATCT TCGCCATCAT CGGACTGGAA 
TTTTATTCGG GTGCATTTCA CAGCGCTTGC TACAACGAAC GCGGTGAAAT TGAAAATGTT 
TCCGAGAGGC CAATGCCGTG CACCAACAAA ACGTCTCCAA TGGGTGTTTA TAACTGTGAT 
GTAAAAGGAA CGACTTGTTT GCAAAAATGG ATAGGTCCTA ATTATGGAAT TACTTCTTTC 
GACAATATCG GATTTGCTAT GATAACTGTG TTTCAGTGCA TTACTATGGA AGGGTGGACC 
ACTGTAATGT ACTACACAAA CGATTCACTT GGAAGCACAT ACAACTGGGC TTACTTTATT 
CCGCTCATTG TTCTAGGTTC TTTTTTCATG CTCAATCTCG TTCTCGGAGT ATTGTCTGGA 
GAGTTCGCCA AGGAAAGAGA ACGTGTTGAA AATCGTCGGG AATTCTTGAA GCTTAGAAGG 
CAACAACAAA TTGAACGAGA GCTTAACGGA TATCTAGAAT GGATTTTAAC AGCCGAAGAG 
GTGATTCTAA AAGAAGATCG AACAACTGAA GAAGAAAAAG CAGCGATCAT GGAAGCCAGA 
CGGCGAGCCG CTAATAAAAA GTTAAAACAA GCCTCAAAAC AGCAGTCTAC AGAAACTGAA 
GAGGACTTTG AGGAGGACGA AGACGAAATG GAAGAGGAAT ACGTCGACGA AGGTGGAACA 
GTAGAAGACG AATTCGCAGA ACGTAAGAAA CGAGGATGTT GCCATTCAGT TGGAAAATTC 
ATCAAACAAC TGAGAATTCA AATCCGTATC ATGGTGAAAA CCCAAATCTT CTACTGGTCT 
GTCATCACAT TGGTCTTTCT AAACACTTGC TGCGTTGCCA GTGAACACTA TGGCCAACCA 
CAATGGTTCA CGGACTTTCT GAAATACGCC GAATTCGTGT TCCTCGGAAT TTTCGTAGTT 
GAAATGCTTT TGAAACTTTT CGCAATGGGA TCAAGAACAT ATTTTGCATC TAAATTCAAC 
CGTTTCGACT GTGTGGTCAT CGTTGGATCA GCTGCTGAAG TAATTTGGGC TGAAGTGTAT 
GGAGGATCAT TCGGTATTTC GGTGATGAGA GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC 
ACATCATACT GGGTGTCACT TCGTAATTTA GTCAGATCAC TCATGAACTC CATGAGGAGT 
ATTATTTCAC TTCTCTTCCT ACTTTTCTTG TTCATTCTCA TTTTCGCCCT TCTTGGAATG 
CAGTTGTTCG GAGGAAGATT CAACTTTCCA ACAATGCACC CTTACACACA CTTTGACACT 
TTTCCAGTTG CTCTCATTAC AGTATTCCAA ATCCTTACTG GAGAAGATTG GAACGAAGTC 
ATGTATCTCG CGATTGAATC ACAAGGAGGT ATCTACAGTG GTGGATGGCC ATACTCTATC 
TACTTTATTG TTTTAGTACT TTTCGGTAAC TACACTTTGT TGAATGTATT CTTGGCTATC 
GCCGTCGACA ACTTAGCCAA CGCTCAGGAA CTCACTGCAG CTGAAGAAGC AGACGAGAAA 
GCAAATGAAA TAGAGGAAGA ATCTGAAGAA CTTGACGAAC AGTACCAAGA AGGTGATCAT 
TGCACCATTG ATATGGAGGG AAAAACAGCT GGAGACATGT GTGCGGTTGC AAGAGCTATG 
GATGATTTGG ACGAAGAATG CGAAGAAGAA GAGTCACCAT TTGGAGGTCC AAAACCCATG 
GTTCCCTACA GTTCTATGTT CTTTCTCTCG CCAACTAATC CATTCCGAGT TCTGATTCAC 
AGTATCGTGT GCACCAAATA CTTTGAAATG ATGGTCATGA CCGTTATCTG TCTCTCATCG 
GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT GAAGAAAATC CGAGAAACAA AGTCCTACAG 
TATATGGATT ACTGTTTTAC TGGAGTTTTT GCATGTGAAA TGCTTCTAAA GTTGATTGAT 
CAAGGTATTC TTCTACATCC CGGATCATAT TGCCGCGATT TCTGGAATAT TCTTGATGGA 
ATTGTTGTAA CATGCGCACT TTTCGCATTT GGTTTTGCAG GTACCGAAGG AAGTGCTGGT 
AAAAATTTGA ACACAATCAA ATCGCTGAGG GTACTTCGAG TGCTTCGTCC ACTCAAAACA 
ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA TTCGATTGTG TTGTTAATTC ATTGAAGAAT 
GTATTCAACA TTCTTATTGT TTATTTTCTA TTTCAATTCA TTTTTGCTGT AATCGCAGTG 
CAGCTTTTCA ACGGGAAATT TTTTTTCTGC ACTGACAAGA ACCGAAAGTT CGCGAACACT 
TGCCACGGGC AGTTCTTTGT CTACGATAAT CAGAATGATC CACCACGGGT TGAACAACGT 
GAATGGCGAT TGAGACCGTT CAACTACGAC AACACAATCA ACGCAATGCT CACATTATTC 
GTTGTAACGA CAGGAGAAGG ATGGCCTGGC ATTCGGCAGA ACAGTATGGA CACAACGTTC 
GAAGATCAGG GTCCAAGTCC ATTTTTTCGG GTTGAAGTGG CTCTATTCTA TGTGATGTTT 
TTCATCGTCT TTCCATTTTT CTTCGTCAAC ATTTTCGTCG CGTTGATTAT CATCACGTTT 
CAAGAACAAG GAGAAGCAGA GTTAAGCGAA GGAGACTTGG ACAAGAACCA AAAACAATGT 
ATCGATTTTG CACTGAATGC TCGGCCAAGA TCACTATTCA TGCCGGAAGA CAAGAACTCT 
ACAAAGTATA GAATATGGAG GCTGGTTACA TCACCGCCGT TTGAATACTT TATCATGACA 
ATGATTTGCT GCAACACGCT CATTCTGATG ATGAAGTACT ACAATAATCC ATTGTTTTAT 
GAAGAAATTT TGAGGCTTTT CAATACCGCA TTAACGGCCG TGTTTACAGT GGAGAGTATA 
TTGAAGATTT TGGCATTCGG TGTTAGAAAT TACTTTCGTG ATGGTTGGAA TCGTTTCGAT 
TTCGTCACCG TCGTCGGTTC TATCACTGAT GCGCTGGTCA CAGAGTTCGG AGGTCATTTT 
GTGTCGCTTG GATTTCTTCG ACTGTTCCGA GCAGCTCGTC TCATTCGTCT CCTGCAGCAA 
GGATATACCA TCCGAATCCT ACTCTGGACG TTCGTTCAAA GTTTCAAAGC TCTACCGTAT 
GTGTGTCTTT TGATTGGAAT GCTCTTCTTC ATTTACGCAA TTGTTGGAAT GCAAGTATTC 
GGGAATATCT GGTTGAATGC TGCCACCGAA ATCAACAGAC ACAACAATTT CCAGTCTTTC 
TTCAACGCAG TTATTTTACT CTTCCGATGC GCGACTGGAG AAGGCTGGCA GGATATTATG 
ATGGCTGCAG TTCAAGGGAA AGATTGCGCA CGAGCCGGCT CTGCTGAAAT AAACTTTGAA 
AAAGGCCAAA CTTGCGGTAG CAACGTTTCA TATGCCTACT TCACAAGTTT CGTTTTCCTC 
TCTTCTTTCT TGATGCTCAA TTTATTCGTT GCCGTCATCA TGGATAATTT TGACTACCTC 
ACAAGAGATT CTTCCATTCT GGGTCCCCAT CATTTAGACG AGTTTATCAG AGTCTGGGCA 
GATTACGATC CAGCAGCAAC GGGCCGAATT CACTATTCCG AAATGTATGA GATGTTAAGA 
ATTATGGCAC CTCCTGTCGG TTTTGGAAAG AAATGTCCAT ACCGATTGGC TTATAAACAT 
CTAATCCGAA TGAACATGCC GGTAGCAGAG GACGGAACTG TGCACTTTAC AACTACCCTG 
TTCGCACTGA TTCGAGAAAG TTTGAGCATA AAAATGAGAC CGGTGGAAGA GATGGATGAA 
GCTGATGAGG AATTAAGATT GACATTAAAG AAAATCTGGC CACTGAAAGC AAAGAAAAAC 
ATGGTTGACT TGGTTGTTCC ACCGAATCAC GAACTTTGCT TCCAAAAGCT CACAGTGGGA 
AAAATCTACG CAGGACTTTT GATTCTCGAA AACTATCGAG CTCGAAAAAG CGGGACGGAG 
GTCGGTGGAC AAGGATTGTT TGGCGGAGGC TTACGATCGT TGGTCGCTGC AGCGAAAGCT 
GCCGAATCGC AACATTCTTC CCACACTCCA CAACCGCCAG AGGAAACCAC TCCGATAATT 
CCACAACATG CACAGCAGTT CTCGGCAGCA CCGACGATGA GCGCACAAGG ATCCTTGCAA 
CAAATGCAGG GAACGAGTAG TGGCGGTGGT CAACGTCCCT ATTCACTCTT CAATAGCTTC 
GTCGACACTA TCAAATCTGG AAAACAGGAT GGAGACGTCA CAGATGTGCA ATATCAAAGT 
GTTGATCAGC AGCACGAAAA GATGAACTCG ACTGGCAGGC GTTTGTCGGA TATGTTCTCG 
AAAATTCGAC GAGGCACATC AGCCGACCAC AATCCGCATC AGACCGAACA TTTGTTGGCT 
CAAGATAATC GATCTCCATC GTCACCTAGA TACCGAAGCA TGGCCCGCGC GTCACCGCCG 
AGTCCTGCTG AACGATACGG TCATCCACCC AGATATCGCA CCGAATCGCC TCCATCTTCG 
AGATCGGAGT ACCAGATGTC AATTAGAGAC CCGATAATTA GGCGTAATCG CTATAATACA 
ATGGAACACT CGCGATCTTC CCACGATCCA CAATATCATC AGGATCAACA ACAACAACAA 
CAACCACATC ACCAGCAACA CTCACAACAT TTGCAACATT CACACCACAA AACGTACCAA 
AATCATAATC AATATTCAAG AAGTCCAATC TATTCCGATG ATTCGAGTGT TGCTGAAAGT 
TATCGAAGAG AGCGAGAATT CCGAAGATAT CAAGACTCCA CTCCACAAGA TGTCAGCGAG 
GACGACGACC CGATGCCTAC GGCGGTCCGA GCGCGCCGAT TACCACTGAT TTCCACAATG 
CCCACGCACT ACGAGTCGGC CTACCAGCCA TCATCATATA ACCAGCATCT CAATGATTCA 
TATGGTTTAG GAACCGGGTA CCAACGAGAT TATCACACTA GTCATTCACA CTCGCACCAT 
CCCACCTCAC AACAACAACA ACATCAGCCC ATGTACAGCA CGTCACCACT AATATCACCG 
AGATCGAGTC ATAGCTACTA CACGCCGAGG AGCTCGCAAT ACTATGAAAT ACCATCGCCG 
AGTCCAGATA TTTATCCATC GTATCGTGGA TCGGCATCAC CTCGCAGGTA CCCCACCAGT 
ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT TCGAGCGCTC GCGTCATTCA AGCTCAGCCT 
GGCTCGATAC CACTATCTGA CTCGGAAACA GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355463.1
CDS	1..6360
Translation	MFVTITSTTIPFSSSTVSFQEPPKSKRSSRDDNSNGYAGYEDATPRPSIDFPTAVIEAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368495.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368495.1

ORF Insert Sequence:

ATGTTTGTGA CTATTACAAG TACCACTATT CCATTCAGTT CTTCAACAGT TTCATTTCAA 
GAACCTCCAA AGTCAAAACG ATCAAGCAGG GATGATAATT CAAATGGGTA TGCAGGGTAT 
GAAGATGCAA CGCCACGACC CTCAATCGAC TTTCCAACTG CAGTTATTGA AGCTGCCCGT 
CGGGCGTCAG TTCTCGGTCG ATCAGCTGCC GAATCGTTAT CTGCTCAAGA AGCAGCATCG 
AGCAGCGAAC GGGGAGAGCA CACTAACAGT CGCTCCCCAT CGACCAGCTA TTCGAGTTGT 
ATCGACGACG AACGAAAGTT CTCGAGTCCT CATCGTAGAC GAGTAGTCGA TGTCAGCGAT 
CATAAGACAT GTGCACTGTT GATGACGAGG ATGAAAGAGG CAAGTAGACA GCTGCCATCG 
CCGAGCCAAC TAGCTGCAGA AGAGGCCCGA AGAGAGCAAA AAGCAGAGTC TGGCACATTC 
GTCAGGAAGA CAACACTTTC ATCCAATGCA CCTGTCAAAG AAAAGGGTCC GTCGTCCCTA 
TTCATCTTCG CCGAGGACAA CATCATTCGT CGAAACGCGA AGGCCATCAT AGAATGGGGA 
CCGTTCGAAT ACTTCATCTT ATTGACAATT ATAGGCAACT GTGTGGTACT ATCCATGGAG 
CAACATCTGC CGAAAAACGA CAAAAAGGCA CTGTCGGAAT GGCTGGAACG AACCGAACCA 
TATTTTATGG GAATTTTTTG TTTAGAATGT GTTTTGAAAG TCATAGCGTT TGGTTTTGCA 
TTACATAAAG GATCATATCT TCGATCAGGA TGGAATATCA TGGATTTTAT TGTAGTAGTT 
TCCGGTGTCG TCACCATGTT ACCATTCTCT CCGGCAACAC AGACCGCCAA CCAACCGGTG 
GATTCTGTCG ATCTTCGAAC ATTGCGAGCT GTTCGAGTGC TTCGACCGCT GAAACTGGTT 
TCTGGAATTC CAAGTTTGCA AGTTGTCCTC AAGTCGATCT TGTGTGCTAT GGCACCATTG 
TTGCAAATTG GATTACTTGT GTTGTTTGCC ATTATCATCT TCGCCATCAT CGGACTGGAA 
TTTTATTCGG GTGCATTTCA CAGCGCTTGC TACAACGAAC GCGGTGAAAT TGAAAATGTT 
TCCGAGAGGC CAATGCCGTG CACCAACAAA ACGTCTCCAA TGGGTGTTTA TAACTGTGAT 
GTAAAAGGAA CGACTTGTTT GCAAAAATGG ATAGGTCCTA ATTATGGAAT TACTTCTTTC 
GACAATATCG GATTTGCTAT GATAACTGTG TTTCAGTGCA TTACTATGGA AGGGTGGACC 
ACTGTAATGT ACTACACAAA CGATTCACTT GGAAGCACAT ACAACTGGGC TTACTTTATT 
CCGCTCATTG TTCTAGGTTC TTTTTTCATG CTCAATCTCG TTCTCGGAGT ATTGTCTGGA 
GAGTTCGCCA AGGAAAGAGA ACGTGTTGAA AATCGTCGGG AATTCTTGAA GCTTAGAAGG 
CAACAACAAA TTGAACGAGA GCTTAACGGA TATCTAGAAT GGATTTTAAC AGCCGAAGAG 
GTGATTCTAA AAGAAGATCG AACAACTGAA GAAGAAAAAG CAGCGATCAT GGAAGCCAGA 
CGGCGAGCCG CTAATAAAAA GTTAAAACAA GCCTCAAAAC AGCAGTCTAC AGAAACTGAA 
GAGGACTTTG AGGAGGACGA AGACGAAATG GAAGAGGAAT ACGTCGACGA AGGTGGAACA 
GTAGAAGACG AATTCGCAGA ACGTAAGAAA CGAGGATGTT GCCATTCAGT TGGAAAATTC 
ATCAAACAAC TGAGAATTCA AATCCGTATC ATGGTGAAAA CCCAAATCTT CTACTGGTCT 
GTCATCACAT TGGTCTTTCT AAACACTTGC TGCGTTGCCA GTGAACACTA TGGCCAACCA 
CAATGGTTCA CGGACTTTCT GAAATACGCC GAATTCGTGT TCCTCGGAAT TTTCGTAGTT 
GAAATGCTTT TGAAACTTTT CGCAATGGGA TCAAGAACAT ATTTTGCATC TAAATTCAAC 
CGTTTCGACT GTGTGGTCAT CGTTGGATCA GCTGCTGAAG TAATTTGGGC TGAAGTGTAT 
GGAGGATCAT TCGGTATTTC GGTGATGAGA GCGCTGAGAC TTCTTCGAAT TTTCAAGCTC 
ACATCATACT GGGTGTCACT TCGTAATTTA GTCAGATCAC TCATGAACTC CATGAGGAGT 
ATTATTTCAC TTCTCTTCCT ACTTTTCTTG TTCATTCTCA TTTTCGCCCT TCTTGGAATG 
CAGTTGTTCG GAGGAAGATT CAACTTTCCA ACAATGCACC CTTACACACA CTTTGACACT 
TTTCCAGTTG CTCTCATTAC AGTATTCCAA ATCCTTACTG GAGAAGATTG GAACGAAGTC 
ATGTATCTCG CGATTGAATC ACAAGGAGGT ATCTACAGTG GTGGATGGCC ATACTCTATC 
TACTTTATTG TTTTAGTACT TTTCGGTAAC TACACTTTGT TGAATGTATT CTTGGCTATC 
GCCGTCGACA ACTTAGCCAA CGCTCAGGAA CTCACTGCAG CTGAAGAAGC AGACGAGAAA 
GCAAATGAAA TAGAGGAAGA ATCTGAAGAA CTTGACGAAC AGTACCAAGA AGGTGATCAT 
TGCACCATTG ATATGGAGGG AAAAACAGCT GGAGACATGT GTGCGGTTGC AAGAGCTATG 
GATGATTTGG ACGAAGAATG CGAAGAAGAA GAGTCACCAT TTGGAGGTCC AAAACCCATG 
GTTCCCTACA GTTCTATGTT CTTTCTCTCG CCAACTAATC CATTCCGAGT TCTGATTCAC 
AGTATCGTGT GCACCAAATA CTTTGAAATG ATGGTCATGA CCGTTATCTG TCTCTCATCG 
GTTTCTCTAG CCGCTGAAGA TCCAGTCGAT GAAGAAAATC CGAGAAACAA AGTCCTACAG 
TATATGGATT ACTGTTTTAC TGGAGTTTTT GCATGTGAAA TGCTTCTAAA GTTGATTGAT 
CAAGGTATTC TTCTACATCC CGGATCATAT TGCCGCGATT TCTGGAATAT TCTTGATGGA 
ATTGTTGTAA CATGCGCACT TTTCGCATTT GGTTTTGCAG GTACCGAAGG AAGTGCTGGT 
AAAAATTTGA ACACAATCAA ATCGCTGAGG GTACTTCGAG TGCTTCGTCC ACTCAAAACA 
ATCAAAAGAA TTCCAAAGCT CAAGGCTGTA TTCGATTGTG TTGTTAATTC ATTGAAGAAT 
GTATTCAACA TTCTTATTGT TTATTTTCTA TTTCAATTCA TTTTTGCTGT AATCGCAGTG 
CAGCTTTTCA ACGGGAAATT TTTTTTCTGC ACTGACAAGA ACCGAAAGTT CGCGAACACT 
TGCCACGGGC AGTTCTTTGT CTACGATAAT CAGAATGATC CACCACGGGT TGAACAACGT 
GAATGGCGAT TGAGACCGTT CAACTACGAC AACACAATCA ACGCAATGCT CACATTATTC 
GTTGTAACGA CAGGAGAAGG ATGGCCTGGC ATTCGGCAGA ACAGTATGGA CACAACGTTC 
GAAGATCAGG GTCCAAGTCC ATTTTTTCGG GTTGAAGTGG CTCTATTCTA TGTGATGTTT 
TTCATCGTCT TTCCATTTTT CTTCGTCAAC ATTTTCGTCG CGTTGATTAT CATCACGTTT 
CAAGAACAAG GAGAAGCAGA GTTAAGCGAA GGAGACTTGG ACAAGAACCA AAAACAATGT 
ATCGATTTTG CACTGAATGC TCGGCCAAGA TCACTATTCA TGCCGGAAGA CAAGAACTCT 
ACAAAGTATA GAATATGGAG GCTGGTTACA TCACCGCCGT TTGAATACTT TATCATGACA 
ATGATTTGCT GCAACACGCT CATTCTGATG ATGAAGTACT ACAATAATCC ATTGTTTTAT 
GAAGAAATTT TGAGGCTTTT CAATACCGCA TTAACGGCCG TGTTTACAGT GGAGAGTATA 
TTGAAGATTT TGGCATTCGG TGTTAGAAAT TACTTTCGTG ATGGTTGGAA TCGTTTCGAT 
TTCGTCACCG TCGTCGGTTC TATCACTGAT GCGCTGGTCA CAGAGTTCGG AGGTCATTTT 
GTGTCGCTTG GATTTCTTCG ACTGTTCCGA GCAGCTCGTC TCATTCGTCT CCTGCAGCAA 
GGATATACCA TCCGAATCCT ACTCTGGACG TTCGTTCAAA GTTTCAAAGC TCTACCGTAT 
GTGTGTCTTT TGATTGGAAT GCTCTTCTTC ATTTACGCAA TTGTTGGAAT GCAAGTATTC 
GGGAATATCT GGTTGAATGC TGCCACCGAA ATCAACAGAC ACAACAATTT CCAGTCTTTC 
TTCAACGCAG TTATTTTACT CTTCCGATGC GCGACTGGAG AAGGCTGGCA GGATATTATG 
ATGGCTGCAG TTCAAGGGAA AGATTGCGCA CGAGCCGGCT CTGCTGAAAT AAACTTTGAA 
AAAGGCCAAA CTTGCGGTAG CAACGTTTCA TATGCCTACT TCACAAGTTT CGTTTTCCTC 
TCTTCTTTCT TGATGCTCAA TTTATTCGTT GCCGTCATCA TGGATAATTT TGACTACCTC 
ACAAGAGATT CTTCCATTCT GGGTCCCCAT CATTTAGACG AGTTTATCAG AGTCTGGGCA 
GATTACGATC CAGCAGCAAC GGGCCGAATT CACTATTCCG AAATGTATGA GATGTTAAGA 
ATTATGGCAC CTCCTGTCGG TTTTGGAAAG AAATGTCCAT ACCGATTGGC TTATAAACAT 
CTAATCCGAA TGAACATGCC GGTAGCAGAG GACGGAACTG TGCACTTTAC AACTACCCTG 
TTCGCACTGA TTCGAGAAAG TTTGAGCATA AAAATGAGAC CGGTGGAAGA GATGGATGAA 
GCTGATGAGG AATTAAGATT GACATTAAAG AAAATCTGGC CACTGAAAGC AAAGAAAAAC 
ATGGTTGACT TGGTTGTTCC ACCGAATCAC GAACTTTGCT TCCAAAAGCT CACAGTGGGA 
AAAATCTACG CAGGACTTTT GATTCTCGAA AACTATCGAG CTCGAAAAAG CGGGACGGAG 
GTCGGTGGAC AAGGATTGTT TGGCGGAGGC TTACGATCGT TGGTCGCTGC AGCGAAAGCT 
GCCGAATCGC AACATTCTTC CCACACTCCA CAACCGCCAG AGGAAACCAC TCCGATAATT 
CCACAACATG CACAGCAGTT CTCGGCAGCA CCGACGATGA GCGCACAAGG ATCCTTGCAA 
CAAATGCAGG GAACGAGTAG TGGCGGTGGT CAACGTCCCT ATTCACTCTT CAATAGCTTC 
GTCGACACTA TCAAATCTGG AAAACAGGAT GGAGACGTCA CAGATGTGCA ATATCAAAGT 
GTTGATCAGC AGCACGAAAA GATGAACTCG ACTGGCAGGC GTTTGTCGGA TATGTTCTCG 
AAAATTCGAC GAGGCACATC AGCCGACCAC AATCCGCATC AGACCGAACA TTTGTTGGCT 
CAAGATAATC GATCTCCATC GTCACCTAGA TACCGAAGCA TGGCCCGCGC GTCACCGCCG 
AGTCCTGCTG AACGATACGG TCATCCACCC AGATATCGCA CCGAATCGCC TCCATCTTCG 
AGATCGGAGT ACCAGATGTC AATTAGAGAC CCGATAATTA GGCGTAATCG CTATAATACA 
ATGGAACACT CGCGATCTTC CCACGATCCA CAATATCATC AGGATCAACA ACAACAACAA 
CAACCACATC ACCAGCAACA CTCACAACAT TTGCAACATT CACACCACAA AACGTACCAA 
AATCATAATC AATATTCAAG AAGTCCAATC TATTCCGATG ATTCGAGTGT TGCTGAAAGT 
TATCGAAGAG AGCGAGAATT CCGAAGATAT CAAGACTCCA CTCCACAAGA TGTCAGCGAG 
GACGACGACC CGATGCCTAC GGCGGTCCGA GCGCGCCGAT TACCACTGAT TTCCACAATG 
CCCACGCACT ACGAGTCGGC CTACCAGCCA TCATCATATA ACCAGCATCT CAATGATTCA 
TATGGTTTAG GAACCGGGTA CCAACGAGAT TATCACACTA GTCATTCACA CTCGCACCAT 
CCCACCTCAC AACAACAACA ACATCAGCCC ATGTACAGCA CGTCACCACT AATATCACCG 
AGATCGAGTC ATAGCTACTA CACGCCGAGG AGCTCGCAAT ACTATGAAAT ACCATCGCCG 
AGTCCAGATA TTTATCCATC GTATCGTGGA TCGGCATCAC CTCGCAGGTA CCCCACCAGT 
ACCGTCGTAG TGGCTCCAGA TCGAGAAGGT TCGAGCGCTC GCGTCATTCA AGCTCAGCCT 
GGCTCGATAC CACTATCTGA CTCGGAAACA GAAGATGATC CTCGATGGGC AATTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368496.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355462.1 Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

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CloneID	OCa698995
Clone ID Related Accession (Same CDS sequence)	NM_001368496.3 , NM_001368496.1
Accession Version	NM_001368496.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_001368496.2. COMPLETENESS: incomplete on the 5' end.

ATGGTGGGCA GGGCTTTTGC CAGGCGGCGC CAATCTATGG CGCCGATAGC AGTCAAAAAG 
CGATCGTCAT CAGCTCATGT GCACCAACCT CCAAAGTCAA AACGATCAAG CAGGGATGAT 
AATTCAAATG GGTATGCAGG GTATGAAGAT GCAACGCCAC GACCCTCAAT CGACTTTCCA 
ACTGCAGTTA TTGAAGCTGC CCGTCGGGCG TCAGTTCTCG GTCGATCAGC TGCCGAATCG 
TTATCTGCTC AAGAAGCAGC ATCGAGCAGC GAACGGGGAG AGCACACTAA CAGTCGCTCC 
CCATCGACCA GCTATTCGAG TTGTATCGAC GACGAACGAA AGTTCTCGAG TCCTCATCGT 
AGACGAGTAG TCGATGTCAG CGATCATAAG ACATGTGCAC TGTTGATGAC GAGGATGAAA 
GAGGCAAGTA GACAGCTGCC ATCGCCGAGC CAACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355462.1
CDS	1..6384
Translation	MVGRAFARRRQSMAPIAVKKRSSSAHVHQPPKSKRSSRDDNSNGYAGYEDATPRPSIDFPTAVIEAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368496.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans EF-hand domain-containing protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368496.3

ORF Insert Sequence:

ATGGTGGGCA GGGCTTTTGC CAGGCGGCGC CAATCTATGG CGCCGATAGC AGTCAAAAAG 
CGATCGTCAT CAGCTCATGT GCACCAACCT CCAAAGTCAA AACGATCAAG CAGGGATGAT 
AATTCAAATG GGTATGCAGG GTATGAAGAT GCAACGCCAC GACCCTCAAT CGACTTTCCA 
ACTGCAGTTA TTGAAGCTGC CCGTCGGGCG TCAGTTCTCG GTCGATCAGC TGCCGAATCG 
TTATCTGCTC AAGAAGCAGC ATCGAGCAGC GAACGGGGAG AGCACACTAA CAGTCGCTCC 
CCATCGACCA GCTATTCGAG TTGTATCGAC GACGAACGAA AGTTCTCGAG TCCTCATCGT 
AGACGAGTAG TCGATGTCAG CGATCATAAG ACATGTGCAC TGTTGATGAC GAGGATGAAA 
GAGGCAAGTA GACAGCTGCC ATCGCCGAGC CAACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-2 ( NM_001368496.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001355462.1 Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

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CloneID	OCa698995
Clone ID Related Accession (Same CDS sequence)	NM_001368496.3 , NM_001368496.1
Accession Version	NM_001368496.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGGTGGGCA GGGCTTTTGC CAGGCGGCGC CAATCTATGG CGCCGATAGC AGTCAAAAAG 
CGATCGTCAT CAGCTCATGT GCACCAACCT CCAAAGTCAA AACGATCAAG CAGGGATGAT 
AATTCAAATG GGTATGCAGG GTATGAAGAT GCAACGCCAC GACCCTCAAT CGACTTTCCA 
ACTGCAGTTA TTGAAGCTGC CCGTCGGGCG TCAGTTCTCG GTCGATCAGC TGCCGAATCG 
TTATCTGCTC AAGAAGCAGC ATCGAGCAGC GAACGGGGAG AGCACACTAA CAGTCGCTCC 
CCATCGACCA GCTATTCGAG TTGTATCGAC GACGAACGAA AGTTCTCGAG TCCTCATCGT 
AGACGAGTAG TCGATGTCAG CGATCATAAG ACATGTGCAC TGTTGATGAC GAGGATGAAA 
GAGGCAAGTA GACAGCTGCC ATCGCCGAGC CAACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355462.1
CDS	1..6384
Translation	MVGRAFARRRQSMAPIAVKKRSSSAHVHQPPKSKRSSRDDNSNGYAGYEDATPRPSIDFPTAVIEAARRASVLGRSAAESLSAQEAASSSERGEHTNSRSPSTSYSSCIDDERKFSSPHRRRVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQEGDHCTIDMEGKTAGDMCAVARAMDDLDEECEEEESPFGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYRARKSGTEVGGQGLFGGGLRSLVAAAKAAESQHSSHTPQPPEETTPIIPQHAQQFSAAPTMSAQGSLQQMQGTSSGGGQRPYSLFNSFVDTIKSGKQDGDVTDVQYQSVDQQHEKMNSTGRRLSDMFSKIRRGTSADHNPHQTEHLLAQDNRSPSSPRYRSMARASPPSPAERYGHPPRYRTESPPSSRSEYQMSIRDPIIRRNRYNTMEHSRSSHDPQYHQDQQQQQQPHHQQHSQHLQHSHHKTYQNHNQYSRSPIYSDDSSVAESYRREREFRRYQDSTPQDVSEDDDPMPTAVRARRLPLISTMPTHYESAYQPSSYNQHLNDSYGLGTGYQRDYHTSHSHSHHPTSQQQQHQPMYSTSPLISPRSSHSYYTPRSSQYYEIPSPSPDIYPSYRGSASPRRYPTSTVVVAPDREGSSARVIQAQPGSIPLSDSETEDDPRWAIV

Target ORF information:

RefSeq Version	NM_001368496.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Uncharacterized protein (unc-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368496.1

ORF Insert Sequence:

ATGGTGGGCA GGGCTTTTGC CAGGCGGCGC CAATCTATGG CGCCGATAGC AGTCAAAAAG 
CGATCGTCAT CAGCTCATGT GCACCAACCT CCAAAGTCAA AACGATCAAG CAGGGATGAT 
AATTCAAATG GGTATGCAGG GTATGAAGAT GCAACGCCAC GACCCTCAAT CGACTTTCCA 
ACTGCAGTTA TTGAAGCTGC CCGTCGGGCG TCAGTTCTCG GTCGATCAGC TGCCGAATCG 
TTATCTGCTC AAGAAGCAGC ATCGAGCAGC GAACGGGGAG AGCACACTAA CAGTCGCTCC 
CCATCGACCA GCTATTCGAG TTGTATCGAC GACGAACGAA AGTTCTCGAG TCCTCATCGT 
AGACGAGTAG TCGATGTCAG CGATCATAAG ACATGTGCAC TGTTGATGAC GAGGATGAAA 
GAGGCAAGTA GACAGCTGCC ATCGCCGAGC CAACTAGCTG CAGAAGAGGC CCGAAGAGAG 
CAAAAAGCAG AGTCTGGCAC ATTCGTCAGG AAGACAACAC TTTCATCCAA TGCACCTGTC 
AAAGAAAAGG GTCCGTCGTC CCTATTCATC TTCGCCGAGG ACAACATCAT TCGTCGAAAC 
GCGAAGGCCA TCATAGAATG GGGACCGTTC GAATACTTCA TCTTATTGAC AATTATAGGC 
AACTGTGTGG TACTATCCAT GGAGCAACAT CTGCCGAAAA ACGACAAAAA GGCACTGTCG 
GAATGGCTGG AACGAACCGA ACCATATTTT ATGGGAATTT TTTGTTTAGA ATGTGTTTTG 
AAAGTCATAG CGTTTGGTTT TGCATTACAT AAAGGATCAT ATCTTCGATC AGGATGGAAT 
ATCATGGATT TTATTGTAGT AGTTTCCGGT GTCGTCACCA TGTTACCATT CTCTCCGGCA 
ACACAGACCG CCAACCAACC GGTGGATTCT GTCGATCTTC GAACATTGCG AGCTGTTCGA 
GTGCTTCGAC CGCTGAAACT GGTTTCTGGA ATTCCAAGTT TGCAAGTTGT CCTCAAGTCG 
ATCTTGTGTG CTATGGCACC ATTGTTGCAA ATTGGATTAC TTGTGTTGTT TGCCATTATC 
ATCTTCGCCA TCATCGGACT GGAATTTTAT TCGGGTGCAT TTCACAGCGC TTGCTACAAC 
GAACGCGGTG AAATTGAAAA TGTTTCCGAG AGGCCAATGC CGTGCACCAA CAAAACGTCT 
CCAATGGGTG TTTATAACTG TGATGTAAAA GGAACGACTT GTTTGCAAAA ATGGATAGGT 
CCTAATTATG GAATTACTTC TTTCGACAAT ATCGGATTTG CTATGATAAC TGTGTTTCAG 
TGCATTACTA TGGAAGGGTG GACCACTGTA ATGTACTACA CAAACGATTC ACTTGGAAGC 
ACATACAACT GGGCTTACTT TATTCCGCTC ATTGTTCTAG GTTCTTTTTT CATGCTCAAT 
CTCGTTCTCG GAGTATTGTC TGGAGAGTTC GCCAAGGAAA GAGAACGTGT TGAAAATCGT 
CGGGAATTCT TGAAGCTTAG AAGGCAACAA CAAATTGAAC GAGAGCTTAA CGGATATCTA 
GAATGGATTT TAACAGCCGA AGAGGTGATT CTAAAAGAAG ATCGAACAAC TGAAGAAGAA 
AAAGCAGCGA TCATGGAAGC CAGACGGCGA GCCGCTAATA AAAAGTTAAA ACAAGCCTCA 
AAACAGCAGT CTACAGAAAC TGAAGAGGAC TTTGAGGAGG ACGAAGACGA AATGGAAGAG 
GAATACGTCG ACGAAGGTGG AACAGTAGAA GACGAATTCG CAGAACGTAA GAAACGAGGA 
TGTTGCCATT CAGTTGGAAA ATTCATCAAA CAACTGAGAA TTCAAATCCG TATCATGGTG 
AAAACCCAAA TCTTCTACTG GTCTGTCATC ACATTGGTCT TTCTAAACAC TTGCTGCGTT 
GCCAGTGAAC ACTATGGCCA ACCACAATGG TTCACGGACT TTCTGAAATA CGCCGAATTC 
GTGTTCCTCG GAATTTTCGT AGTTGAAATG CTTTTGAAAC TTTTCGCAAT GGGATCAAGA 
ACATATTTTG CATCTAAATT CAACCGTTTC GACTGTGTGG TCATCGTTGG ATCAGCTGCT 
GAAGTAATTT GGGCTGAAGT GTATGGAGGA TCATTCGGTA TTTCGGTGAT GAGAGCGCTG 
AGACTTCTTC GAATTTTCAA GCTCACATCA TACTGGGTGT CACTTCGTAA TTTAGTCAGA 
TCACTCATGA ACTCCATGAG GAGTATTATT TCACTTCTCT TCCTACTTTT CTTGTTCATT 
CTCATTTTCG CCCTTCTTGG AATGCAGTTG TTCGGAGGAA GATTCAACTT TCCAACAATG 
CACCCTTACA CACACTTTGA CACTTTTCCA GTTGCTCTCA TTACAGTATT CCAAATCCTT 
ACTGGAGAAG ATTGGAACGA AGTCATGTAT CTCGCGATTG AATCACAAGG AGGTATCTAC 
AGTGGTGGAT GGCCATACTC TATCTACTTT ATTGTTTTAG TACTTTTCGG TAACTACACT 
TTGTTGAATG TATTCTTGGC TATCGCCGTC GACAACTTAG CCAACGCTCA GGAACTCACT 
GCAGCTGAAG AAGCAGACGA GAAAGCAAAT GAAATAGAGG AAGAATCTGA AGAACTTGAC 
GAACAGTACC AAGAAGGTGA TCATTGCACC ATTGATATGG AGGGAAAAAC AGCTGGAGAC 
ATGTGTGCGG TTGCAAGAGC TATGGATGAT TTGGACGAAG AATGCGAAGA AGAAGAGTCA 
CCATTTGGAG GTCCAAAACC CATGGTTCCC TACAGTTCTA TGTTCTTTCT CTCGCCAACT 
AATCCATTCC GAGTTCTGAT TCACAGTATC GTGTGCACCA AATACTTTGA AATGATGGTC 
ATGACCGTTA TCTGTCTCTC ATCGGTTTCT CTAGCCGCTG AAGATCCAGT CGATGAAGAA 
AATCCGAGAA ACAAAGTCCT ACAGTATATG GATTACTGTT TTACTGGAGT TTTTGCATGT 
GAAATGCTTC TAAAGTTGAT TGATCAAGGT ATTCTTCTAC ATCCCGGATC ATATTGCCGC 
GATTTCTGGA ATATTCTTGA TGGAATTGTT GTAACATGCG CACTTTTCGC ATTTGGTTTT 
GCAGGTACCG AAGGAAGTGC TGGTAAAAAT TTGAACACAA TCAAATCGCT GAGGGTACTT 
CGAGTGCTTC GTCCACTCAA AACAATCAAA AGAATTCCAA AGCTCAAGGC TGTATTCGAT 
TGTGTTGTTA ATTCATTGAA GAATGTATTC AACATTCTTA TTGTTTATTT TCTATTTCAA 
TTCATTTTTG CTGTAATCGC AGTGCAGCTT TTCAACGGGA AATTTTTTTT CTGCACTGAC 
AAGAACCGAA AGTTCGCGAA CACTTGCCAC GGGCAGTTCT TTGTCTACGA TAATCAGAAT 
GATCCACCAC GGGTTGAACA ACGTGAATGG CGATTGAGAC CGTTCAACTA CGACAACACA 
ATCAACGCAA TGCTCACATT ATTCGTTGTA ACGACAGGAG AAGGATGGCC TGGCATTCGG 
CAGAACAGTA TGGACACAAC GTTCGAAGAT CAGGGTCCAA GTCCATTTTT TCGGGTTGAA 
GTGGCTCTAT TCTATGTGAT GTTTTTCATC GTCTTTCCAT TTTTCTTCGT CAACATTTTC 
GTCGCGTTGA TTATCATCAC GTTTCAAGAA CAAGGAGAAG CAGAGTTAAG CGAAGGAGAC 
TTGGACAAGA ACCAAAAACA ATGTATCGAT TTTGCACTGA ATGCTCGGCC AAGATCACTA 
TTCATGCCGG AAGACAAGAA CTCTACAAAG TATAGAATAT GGAGGCTGGT TACATCACCG 
CCGTTTGAAT ACTTTATCAT GACAATGATT TGCTGCAACA CGCTCATTCT GATGATGAAG 
TACTACAATA ATCCATTGTT TTATGAAGAA ATTTTGAGGC TTTTCAATAC CGCATTAACG 
GCCGTGTTTA CAGTGGAGAG TATATTGAAG ATTTTGGCAT TCGGTGTTAG AAATTACTTT 
CGTGATGGTT GGAATCGTTT CGATTTCGTC ACCGTCGTCG GTTCTATCAC TGATGCGCTG 
GTCACAGAGT TCGGAGGTCA TTTTGTGTCG CTTGGATTTC TTCGACTGTT CCGAGCAGCT 
CGTCTCATTC GTCTCCTGCA GCAAGGATAT ACCATCCGAA TCCTACTCTG GACGTTCGTT 
CAAAGTTTCA AAGCTCTACC GTATGTGTGT CTTTTGATTG GAATGCTCTT CTTCATTTAC 
GCAATTGTTG GAATGCAAGT ATTCGGGAAT ATCTGGTTGA ATGCTGCCAC CGAAATCAAC 
AGACACAACA ATTTCCAGTC TTTCTTCAAC GCAGTTATTT TACTCTTCCG ATGCGCGACT 
GGAGAAGGCT GGCAGGATAT TATGATGGCT GCAGTTCAAG GGAAAGATTG CGCACGAGCC 
GGCTCTGCTG AAATAAACTT TGAAAAAGGC CAAACTTGCG GTAGCAACGT TTCATATGCC 
TACTTCACAA GTTTCGTTTT CCTCTCTTCT TTCTTGATGC TCAATTTATT CGTTGCCGTC 
ATCATGGATA ATTTTGACTA CCTCACAAGA GATTCTTCCA TTCTGGGTCC CCATCATTTA 
GACGAGTTTA TCAGAGTCTG GGCAGATTAC GATCCAGCAG CAACGGGCCG AATTCACTAT 
TCCGAAATGT ATGAGATGTT AAGAATTATG GCACCTCCTG TCGGTTTTGG AAAGAAATGT 
CCATACCGAT TGGCTTATAA ACATCTAATC CGAATGAACA TGCCGGTAGC AGAGGACGGA 
ACTGTGCACT TTACAACTAC CCTGTTCGCA CTGATTCGAG AAAGTTTGAG CATAAAAATG 
AGACCGGTGG AAGAGATGGA TGAAGCTGAT GAGGAATTAA GATTGACATT AAAGAAAATC 
TGGCCACTGA AAGCAAAGAA AAACATGGTT GACTTGGTTG TTCCACCGAA TCACGAACTT 
TGCTTCCAAA AGCTCACAGT GGGAAAAATC TACGCAGGAC TTTTGATTCT CGAAAACTAT 
CGAGCTCGAA AAAGCGGGAC GGAGGTCGGT GGACAAGGAT TGTTTGGCGG AGGCTTACGA 
TCGTTGGTCG CTGCAGCGAA AGCTGCCGAA TCGCAACATT CTTCCCACAC TCCACAACCG 
CCAGAGGAAA CCACTCCGAT AATTCCACAA CATGCACAGC AGTTCTCGGC AGCACCGACG 
ATGAGCGCAC AAGGATCCTT GCAACAAATG CAGGGAACGA GTAGTGGCGG TGGTCAACGT 
CCCTATTCAC TCTTCAATAG CTTCGTCGAC ACTATCAAAT CTGGAAAACA GGATGGAGAC 
GTCACAGATG TGCAATATCA AAGTGTTGAT CAGCAGCACG AAAAGATGAA CTCGACTGGC 
AGGCGTTTGT CGGATATGTT CTCGAAAATT CGACGAGGCA CATCAGCCGA CCACAATCCG 
CATCAGACCG AACATTTGTT GGCTCAAGAT AATCGATCTC CATCGTCACC TAGATACCGA 
AGCATGGCCC GCGCGTCACC GCCGAGTCCT GCTGAACGAT ACGGTCATCC ACCCAGATAT 
CGCACCGAAT CGCCTCCATC TTCGAGATCG GAGTACCAGA TGTCAATTAG AGACCCGATA 
ATTAGGCGTA ATCGCTATAA TACAATGGAA CACTCGCGAT CTTCCCACGA TCCACAATAT 
CATCAGGATC AACAACAACA ACAACAACCA CATCACCAGC AACACTCACA ACATTTGCAA 
CATTCACACC ACAAAACGTA CCAAAATCAT AATCAATATT CAAGAAGTCC AATCTATTCC 
GATGATTCGA GTGTTGCTGA AAGTTATCGA AGAGAGCGAG AATTCCGAAG ATATCAAGAC 
TCCACTCCAC AAGATGTCAG CGAGGACGAC GACCCGATGC CTACGGCGGT CCGAGCGCGC 
CGATTACCAC TGATTTCCAC AATGCCCACG CACTACGAGT CGGCCTACCA GCCATCATCA 
TATAACCAGC ATCTCAATGA TTCATATGGT TTAGGAACCG GGTACCAACG AGATTATCAC 
ACTAGTCATT CACACTCGCA CCATCCCACC TCACAACAAC AACAACATCA GCCCATGTAC 
AGCACGTCAC CACTAATATC ACCGAGATCG AGTCATAGCT ACTACACGCC GAGGAGCTCG 
CAATACTATG AAATACCATC GCCGAGTCCA GATATTTATC CATCGTATCG TGGATCGGCA 
TCACCTCGCA GGTACCCCAC CAGTACCGTC GTAGTGGCTC CAGATCGAGA AGGTTCGAGC 
GCTCGCGTCA TTCAAGCTCA GCCTGGCTCG ATACCACTAT CTGACTCGGA AACAGAAGAT 
GATCCTCGAT GGGCAATTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	unc-2
Entrez Gene ID	180570
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

unc-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

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