rocf-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	rocf-1
Entrez Gene ID	180041
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_074447.4	NP_506848.1	ZP domain-containing protein
NM_074447.3	NP_506848.1	regulator of Rme (RME) Circuit formation

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rocf-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rocf-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa354901	NM_074447.4 Latest version!	Caenorhabditis elegans ZP domain-containing protein (rocf-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa354901	NM_074447.3	Caenorhabditis elegans regulator of Rme (RME) Circuit formation (rocf-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rocf-1 ( NM_074447.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_506848.1 Caenorhabditis elegans ZP domain-containing protein (rocf-1), mRNA.

Price & Availability↑

CloneID	OCa354901
Clone ID Related Accession (Same CDS sequence)	NM_074447.4 , NM_074447.3
Accession Version	NM_074447.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ZP domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_074447.3.

ATGAGGCCCT TACTCTCATG TTTCCCCGTG ATTTTCGCGG TTTTTGGGCC GATTATTGCG 
GTTAAGCCCG TATGGAAGAC GCCCGTCTCT GAGCCGAATC CAAATGCGAA TCTCGAAGTT 
TTAAAGGGAC ACTCGAATTT GGCGAAGCGT GCTCAACCGA ACTACTACAC ACGTCCCGAG 
CAGGTCTCGA TTCCGGAATA CCGTCCACCT CGTCAACGTC TTCCACCGTT ACCATCTCCA 
AAGATCTCAT ATCAACAAGT TCCTCCACAT CAACTAACAT CTGGTGGAAG CTTCCGACCA 
TTCGCTTGGG TTGGCCCTCA ACCAAAACCC GGCAGCCCTC AAACCAGTTA CTATGAGAAT 
TCTCCAAACA AGGGCCAATT TCCAAAGGGA TTACCTCCAT TGCCACCAAA TTGGCAGCAG 
TATCCAGTGC AGATGATATG GATTCCAGAC ACGCCAGGAC AAACTACTGG AGCGTACCCG 
TATTATCCGG GACCCGCTGG ACCGCCCGTG CCAGTTTCAA CAGGAAGCTA TGGCCTGTAT 
GGACCAACTG TGACAACCGA GAAGCCAATG GAACCGTCGG TCAATCCGGA AACGTATACA 
GGAACCGTCG CTCACAGTCC CTCTGCCAAC CCTATCACCA CTACTATACC TTACGAGCCC 
CCAAATCCAT ATGGAGGACA GACTCAGGTG TCCCCGTCTT CAAATTCCTA TCCGGATTCA 
ACAGTCTCCA CTACTTCGAG CAACACGGAA TCCTATGAAC AGCCATCTAC ATCTCCCTCA 
GAAAGGCCTA GATCATCAAC TCGGGATTTT GAAAATGGAA ATGGAAAAAC TGTAAATCCA 
ACCATCCGCC CAGGACCTCA AGAGCTTAGT GGTGGAATTG GAACTTCTTC CAAGCCTAGA 
CTGCCTAATG ACGCCGACTA CACTAGAGAT GTAGAGACTG GAAGCTCTGA AGTTACAACT 
CCGTTTGGTA GAAACACGGA AAGCTCTACT CCTTCTAAAA GCCCAGGATC AACAAGTTCA 
TCCGACACCA CTCCGTCTTC TAACGAAAAT GGGAAAGAGA CAACTCCATT CGAGATGACC 
AAAAATCCAT TCAACGATCT TAAAACCATC CGACCAATAT ATCTAGGTTC AAGTTCTGAC 
GAAGAAGATG ACGAACCGCT AGAGGATATC AGCAAGGCAA CCGATTCCCC ACATTCTTCA 
GAGAAATCTC AGATTCCTGG ATCACCAGGT TCACAGATAA AGAATCCATT GAAACCCTCT 
TCAACGGAAT CGGAAACTAT TGGACCGGAA TCCTCCGAGT CAGAAGACGA CGGAGAGCGA 
AGCACGCCAA CTCCACAAGA GGAGGAAGAG GAAATGATGA GGAACTTCTT CCCAAGACCT 
GTCAGTGCTC AAGCATATCA GCCGCCAGGA CATCAGCCGG AAGATTTTAC GAGTCATATC 
AACGTGAATT TCAAGAGACC AAAAGGCAAA GGAGGATGTG TAACACCGCC GTGCAGAGGA 
GTGGATACTG ATAAGATATC CCTCTACAAG CCAGACCGTG ACAATGAGGA CTCGAGCCCG 
ACACTCGTGA TCAGTAACGC TGCAAAAGAC GATGCATCGC GTGCCGGATA CAAACCAGCT 
CCACCACTGA TCAATCCATA CATCCCTCCA CCACCGCCCC CGCATCCTGA AGAAGAGTAT 
GCCACGCCGT ACACCGTTCC CCCCGGCAAA CAACCATTCC CACCATTTGG AGAAGGACCT 
GCGACTCCGC CAAGAAGCCT GATCCCGGCG CATAATTTCC CTCCTCCAAG TACTTTCCCA 
CCTGATGAGG ATTACCACGA GACTACGACA GATACCGTCC GTCCAACTGC TCAGACTATT 
TCTACTAGAT TTACTGCCCC GACAACTACC ACAACATCAA AGCCCGTTGA ATCCACAACT 
ACCGAGGAAG ATACTCCGGA GCATGTTCCA CCTGCAATTG TTCATATGAC CGCGTCGACG 
CCAAGTGGGA AGAACCCTCC AACCTATATT CCACTGCATC CAGAGACATC CCAGTCCACA 
CCAACTCCAA CAACATCTGC TAGATCCACG AGACCGACAG CTTCAGTCAC AAGACGACCC 
GAGTTCCCAA CTAGAAATAC TCCACCATTT GGAGGAAACG TGCCAAATGG GCCGTCTTCT 
ACGGTACCAC CTGCCAGCAA TCCAACCACT GAAGGATACT TCACACAAGT CTTCACTGAC 
CGAGAGAAGA CAAATTCCGG AGAGTCTGTA GTGACCACCA CAGCTTCATC TACTGATTCT 
GAAACTCCAG GCTCTGGATC ATTCTCGACA TCACCTCAAA CCACTGCTGG ATGGATCTAC 
ACATCTACCG ACGCTACACC AGAATCTGAA TACTCCACTG CATCAAGCTT CCCAACTCTA 
TCAACTTCTG ATCGTTTCAC ACCGTCTGAA TCATCTAGAA GCACGGAGAC CACCACCGTC 
ATCACGCCGT TCACTGAGAC ATTCGAGTCT TCTACACTGA CTCCTGATCA ATTCGAAGAT 
GTGACCTCTA CATTTACAGA TAGATTCAGA CCGTCGGAAT ATGCCACGTC ACCTACACCT 
CCTTCGCCGA CTCGCGTCGT CACCGCAAGC TTCCCGCCGA ACGAGGGATC ATCACCTGGA 
ATCTTGCCAA GCACCAACCC GCCACCCAGA AATAATCCAT ATGCTCCTCC AGAGGGTGCT 
CCAAGGGTAC CAAATCGGTT GATTGGAAAG CCGAGAATCC TCTGTCTGGA GGATGGAATC 
AGTTTCGAGG TTAAAACCAT TTTGCCGCTC ACCGGAGAAG TCTTTGCAAA TGATAGGAAA 
CGGGTTCTAG AATGCCACAA ATCCTTCACT GAAGAGGCCA AGCCAAAAAT GTTCCTGCCA 
TTTTCCACGT GTGGAGTCAA GAATGTTGGT GAACAAGTGG ACAGCAGAGC TCAATACCAC 
ATGCAAGTGG TTCTCATCAT TGATCAAGGA AATGGCACGA ACACTTTGCA ATCATTTATG 
GCTCAATGTG TCCATCAGAA GGTCAATTAT AACAAGCAAG TGCTCCCGAA GAGAATTGAA 
GAGGCTCTGG AAGAACTCCG CTTAGTACCA TCAAAGTTGG AGCAGAAAGC CTCTATGCCG 
AGTGTTCAAA TGCAAATTGT AGTAGACGAG GGACATCACA AGCTTGGCGC TGAGGTCAGT 
GCTGCTGACA TTGGAATGCC ACTGGCTCTT AAATGGAGTA TGGTTCCAGA GTCGGACGCA 
TATGGAATGC ACATACGCAA CTGCAAAGTG GTAGATGCGG TGGGCAAGAT CGATCATACT 
CTAATAGACG AGCAGGGGTG CTCTGCGGAT CTTCAGATCA TCGATCATCC CCATTATGAC 
ACCTACCACG ACACTGCTTC CTCTCACATG TGGGCTTTCA AGGTTCCAGA CATGTCGAGC 
CTCCAGATCA AATGTGATAT TCTGATATGC TCAAACATCA AGTCTTCCGT TACGAACACG 
ACTAGCTGTG AGGATATTCC TTCGCCTCCA TTCTGTGCAG ATGTTGTGAC GTCTCCACCA 
AATTCTATCC TCTCAGATGC TTCTTCATTC ATCAAGCATC GACGTGCTTC AGTACCTTCT 
GAAGTTTCCC CAGCGACGCA ATCGGTGAGA ACCTCGATTT GCTTGTCGAA AAGCTGCAGA 
CCAGATTTTT CAGAAGAAGT CCGAATCTGC GTCAACACTC AATTGGCGAC TACTTCGACA 
GGATTGTCAT TGGCGTTCTT ACTTTTTGCA ATTTCTTTCC AAATTATCGC AAGAACAAAG 
CGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_506848.1
CDS	75..3863
Translation	MRPLLSCFPVIFAVFGPIIAVKPVWKTPVSEPNPNANLEVLKGHSNLAKRAQPNYYTRPEQVSIPEYRPPRQRLPPLPSPKISYQQVPPHQLTSGGSFRPFAWVGPQPKPGSPQTSYYENSPNKGQFPKGLPPLPPNWQQYPVQMIWIPDTPGQTTGAYPYYPGPAGPPVPVSTGSYGLYGPTVTTEKPMEPSVNPETYTGTVAHSPSANPITTTIPYEPPNPYGGQTQVSPSSNSYPDSTVSTTSSNTESYEQPSTSPSERPRSSTRDFENGNGKTVNPTIRPGPQELSGGIGTSSKPRLPNDADYTRDVETGSSEVTTPFGRNTESSTPSKSPGSTSSSDTTPSSNENGKETTPFEMTKNPFNDLKTIRPIYLGSSSDEEDDEPLEDISKATDSPHSSEKSQIPGSPGSQIKNPLKPSSTESETIGPESSESEDDGERSTPTPQEEEEEMMRNFFPRPVSAQAYQPPGHQPEDFTSHINVNFKRPKGKGGCVTPPCRGVDTDKISLYKPDRDNEDSSPTLVISNAAKDDASRAGYKPAPPLINPYIPPPPPPHPEEEYATPYTVPPGKQPFPPFGEGPATPPRSLIPAHNFPPPSTFPPDEDYHETTTDTVRPTAQTISTRFTAPTTTTTSKPVESTTTEEDTPEHVPPAIVHMTASTPSGKNPPTYIPLHPETSQSTPTPTTSARSTRPTASVTRRPEFPTRNTPPFGGNVPNGPSSTVPPASNPTTEGYFTQVFTDREKTNSGESVVTTTASSTDSETPGSGSFSTSPQTTAGWIYTSTDATPESEYSTASSFPTLSTSDRFTPSESSRSTETTTVITPFTETFESSTLTPDQFEDVTSTFTDRFRPSEYATSPTPPSPTRVVTASFPPNEGSSPGILPSTNPPPRNNPYAPPEGAPRVPNRLIGKPRILCLEDGISFEVKTILPLTGEVFANDRKRVLECHKSFTEEAKPKMFLPFSTCGVKNVGEQVDSRAQYHMQVVLIIDQGNGTNTLQSFMAQCVHQKVNYNKQVLPKRIEEALEELRLVPSKLEQKASMPSVQMQIVVDEGHHKLGAEVSAADIGMPLALKWSMVPESDAYGMHIRNCKVVDAVGKIDHTLIDEQGCSADLQIIDHPHYDTYHDTASSHMWAFKVPDMSSLQIKCDILICSNIKSSVTNTTSCEDIPSPPFCADVVTSPPNSILSDASSFIKHRRASVPSEVSPATQSVRTSICLSKSCRPDFSEEVRICVNTQLATTSTGLSLAFLLFAISFQIIARTKRP

Target ORF information:

RefSeq Version	NM_074447.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ZP domain-containing protein (rocf-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_074447.4

ORF Insert Sequence:

ATGAGGCCCT TACTCTCATG TTTCCCCGTG ATTTTCGCGG TTTTTGGGCC GATTATTGCG 
GTTAAGCCCG TATGGAAGAC GCCCGTCTCT GAGCCGAATC CAAATGCGAA TCTCGAAGTT 
TTAAAGGGAC ACTCGAATTT GGCGAAGCGT GCTCAACCGA ACTACTACAC ACGTCCCGAG 
CAGGTCTCGA TTCCGGAATA CCGTCCACCT CGTCAACGTC TTCCACCGTT ACCATCTCCA 
AAGATCTCAT ATCAACAAGT TCCTCCACAT CAACTAACAT CTGGTGGAAG CTTCCGACCA 
TTCGCTTGGG TTGGCCCTCA ACCAAAACCC GGCAGCCCTC AAACCAGTTA CTATGAGAAT 
TCTCCAAACA AGGGCCAATT TCCAAAGGGA TTACCTCCAT TGCCACCAAA TTGGCAGCAG 
TATCCAGTGC AGATGATATG GATTCCAGAC ACGCCAGGAC AAACTACTGG AGCGTACCCG 
TATTATCCGG GACCCGCTGG ACCGCCCGTG CCAGTTTCAA CAGGAAGCTA TGGCCTGTAT 
GGACCAACTG TGACAACCGA GAAGCCAATG GAACCGTCGG TCAATCCGGA AACGTATACA 
GGAACCGTCG CTCACAGTCC CTCTGCCAAC CCTATCACCA CTACTATACC TTACGAGCCC 
CCAAATCCAT ATGGAGGACA GACTCAGGTG TCCCCGTCTT CAAATTCCTA TCCGGATTCA 
ACAGTCTCCA CTACTTCGAG CAACACGGAA TCCTATGAAC AGCCATCTAC ATCTCCCTCA 
GAAAGGCCTA GATCATCAAC TCGGGATTTT GAAAATGGAA ATGGAAAAAC TGTAAATCCA 
ACCATCCGCC CAGGACCTCA AGAGCTTAGT GGTGGAATTG GAACTTCTTC CAAGCCTAGA 
CTGCCTAATG ACGCCGACTA CACTAGAGAT GTAGAGACTG GAAGCTCTGA AGTTACAACT 
CCGTTTGGTA GAAACACGGA AAGCTCTACT CCTTCTAAAA GCCCAGGATC AACAAGTTCA 
TCCGACACCA CTCCGTCTTC TAACGAAAAT GGGAAAGAGA CAACTCCATT CGAGATGACC 
AAAAATCCAT TCAACGATCT TAAAACCATC CGACCAATAT ATCTAGGTTC AAGTTCTGAC 
GAAGAAGATG ACGAACCGCT AGAGGATATC AGCAAGGCAA CCGATTCCCC ACATTCTTCA 
GAGAAATCTC AGATTCCTGG ATCACCAGGT TCACAGATAA AGAATCCATT GAAACCCTCT 
TCAACGGAAT CGGAAACTAT TGGACCGGAA TCCTCCGAGT CAGAAGACGA CGGAGAGCGA 
AGCACGCCAA CTCCACAAGA GGAGGAAGAG GAAATGATGA GGAACTTCTT CCCAAGACCT 
GTCAGTGCTC AAGCATATCA GCCGCCAGGA CATCAGCCGG AAGATTTTAC GAGTCATATC 
AACGTGAATT TCAAGAGACC AAAAGGCAAA GGAGGATGTG TAACACCGCC GTGCAGAGGA 
GTGGATACTG ATAAGATATC CCTCTACAAG CCAGACCGTG ACAATGAGGA CTCGAGCCCG 
ACACTCGTGA TCAGTAACGC TGCAAAAGAC GATGCATCGC GTGCCGGATA CAAACCAGCT 
CCACCACTGA TCAATCCATA CATCCCTCCA CCACCGCCCC CGCATCCTGA AGAAGAGTAT 
GCCACGCCGT ACACCGTTCC CCCCGGCAAA CAACCATTCC CACCATTTGG AGAAGGACCT 
GCGACTCCGC CAAGAAGCCT GATCCCGGCG CATAATTTCC CTCCTCCAAG TACTTTCCCA 
CCTGATGAGG ATTACCACGA GACTACGACA GATACCGTCC GTCCAACTGC TCAGACTATT 
TCTACTAGAT TTACTGCCCC GACAACTACC ACAACATCAA AGCCCGTTGA ATCCACAACT 
ACCGAGGAAG ATACTCCGGA GCATGTTCCA CCTGCAATTG TTCATATGAC CGCGTCGACG 
CCAAGTGGGA AGAACCCTCC AACCTATATT CCACTGCATC CAGAGACATC CCAGTCCACA 
CCAACTCCAA CAACATCTGC TAGATCCACG AGACCGACAG CTTCAGTCAC AAGACGACCC 
GAGTTCCCAA CTAGAAATAC TCCACCATTT GGAGGAAACG TGCCAAATGG GCCGTCTTCT 
ACGGTACCAC CTGCCAGCAA TCCAACCACT GAAGGATACT TCACACAAGT CTTCACTGAC 
CGAGAGAAGA CAAATTCCGG AGAGTCTGTA GTGACCACCA CAGCTTCATC TACTGATTCT 
GAAACTCCAG GCTCTGGATC ATTCTCGACA TCACCTCAAA CCACTGCTGG ATGGATCTAC 
ACATCTACCG ACGCTACACC AGAATCTGAA TACTCCACTG CATCAAGCTT CCCAACTCTA 
TCAACTTCTG ATCGTTTCAC ACCGTCTGAA TCATCTAGAA GCACGGAGAC CACCACCGTC 
ATCACGCCGT TCACTGAGAC ATTCGAGTCT TCTACACTGA CTCCTGATCA ATTCGAAGAT 
GTGACCTCTA CATTTACAGA TAGATTCAGA CCGTCGGAAT ATGCCACGTC ACCTACACCT 
CCTTCGCCGA CTCGCGTCGT CACCGCAAGC TTCCCGCCGA ACGAGGGATC ATCACCTGGA 
ATCTTGCCAA GCACCAACCC GCCACCCAGA AATAATCCAT ATGCTCCTCC AGAGGGTGCT 
CCAAGGGTAC CAAATCGGTT GATTGGAAAG CCGAGAATCC TCTGTCTGGA GGATGGAATC 
AGTTTCGAGG TTAAAACCAT TTTGCCGCTC ACCGGAGAAG TCTTTGCAAA TGATAGGAAA 
CGGGTTCTAG AATGCCACAA ATCCTTCACT GAAGAGGCCA AGCCAAAAAT GTTCCTGCCA 
TTTTCCACGT GTGGAGTCAA GAATGTTGGT GAACAAGTGG ACAGCAGAGC TCAATACCAC 
ATGCAAGTGG TTCTCATCAT TGATCAAGGA AATGGCACGA ACACTTTGCA ATCATTTATG 
GCTCAATGTG TCCATCAGAA GGTCAATTAT AACAAGCAAG TGCTCCCGAA GAGAATTGAA 
GAGGCTCTGG AAGAACTCCG CTTAGTACCA TCAAAGTTGG AGCAGAAAGC CTCTATGCCG 
AGTGTTCAAA TGCAAATTGT AGTAGACGAG GGACATCACA AGCTTGGCGC TGAGGTCAGT 
GCTGCTGACA TTGGAATGCC ACTGGCTCTT AAATGGAGTA TGGTTCCAGA GTCGGACGCA 
TATGGAATGC ACATACGCAA CTGCAAAGTG GTAGATGCGG TGGGCAAGAT CGATCATACT 
CTAATAGACG AGCAGGGGTG CTCTGCGGAT CTTCAGATCA TCGATCATCC CCATTATGAC 
ACCTACCACG ACACTGCTTC CTCTCACATG TGGGCTTTCA AGGTTCCAGA CATGTCGAGC 
CTCCAGATCA AATGTGATAT TCTGATATGC TCAAACATCA AGTCTTCCGT TACGAACACG 
ACTAGCTGTG AGGATATTCC TTCGCCTCCA TTCTGTGCAG ATGTTGTGAC GTCTCCACCA 
AATTCTATCC TCTCAGATGC TTCTTCATTC ATCAAGCATC GACGTGCTTC AGTACCTTCT 
GAAGTTTCCC CAGCGACGCA ATCGGTGAGA ACCTCGATTT GCTTGTCGAA AAGCTGCAGA 
CCAGATTTTT CAGAAGAAGT CCGAATCTGC GTCAACACTC AATTGGCGAC TACTTCGACA 
GGATTGTCAT TGGCGTTCTT ACTTTTTGCA ATTTCTTTCC AAATTATCGC AAGAACAAAG 
CGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rocf-1 ( NM_074447.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_506848.1 Caenorhabditis elegans regulator of Rme (RME) Circuit formation (rocf-1), partial mRNA.

Price & Availability↑

CloneID	OCa354901
Clone ID Related Accession (Same CDS sequence)	NM_074447.4 , NM_074447.3
Accession Version	NM_074447.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	regulator of Rme (RME) Circuit formation
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_074447.2. COMPLETENESS: incomplete on both ends.

ATGAGGCCCT TACTCTCATG TTTCCCCGTG ATTTTCGCGG TTTTTGGGCC GATTATTGCG 
GTTAAGCCCG TATGGAAGAC GCCCGTCTCT GAGCCGAATC CAAATGCGAA TCTCGAAGTT 
TTAAAGGGAC ACTCGAATTT GGCGAAGCGT GCTCAACCGA ACTACTACAC ACGTCCCGAG 
CAGGTCTCGA TTCCGGAATA CCGTCCACCT CGTCAACGTC TTCCACCGTT ACCATCTCCA 
AAGATCTCAT ATCAACAAGT TCCTCCACAT CAACTAACAT CTGGTGGAAG CTTCCGACCA 
TTCGCTTGGG TTGGCCCTCA ACCAAAACCC GGCAGCCCTC AAACCAGTTA CTATGAGAAT 
TCTCCAAACA AGGGCCAATT TCCAAAGGGA TTACCTCCAT TGCCACCAAA TTGGCAGCAG 
TATCCAGTGC AGATGATATG GATTCCAGAC ACGCCAGGAC AAACTACTGG AGCGTACCCG 
TATTATCCGG GACCCGCTGG ACCGCCCGTG CCAGTTTCAA CAGGAAGCTA TGGCCTGTAT 
GGACCAACTG TGACAACCGA GAAGCCAATG GAACCGTCGG TCAATCCGGA AACGTATACA 
GGAACCGTCG CTCACAGTCC CTCTGCCAAC CCTATCACCA CTACTATACC TTACGAGCCC 
CCAAATCCAT ATGGAGGACA GACTCAGGTG TCCCCGTCTT CAAATTCCTA TCCGGATTCA 
ACAGTCTCCA CTACTTCGAG CAACACGGAA TCCTATGAAC AGCCATCTAC ATCTCCCTCA 
GAAAGGCCTA GATCATCAAC TCGGGATTTT GAAAATGGAA ATGGAAAAAC TGTAAATCCA 
ACCATCCGCC CAGGACCTCA AGAGCTTAGT GGTGGAATTG GAACTTCTTC CAAGCCTAGA 
CTGCCTAATG ACGCCGACTA CACTAGAGAT GTAGAGACTG GAAGCTCTGA AGTTACAACT 
CCGTTTGGTA GAAACACGGA AAGCTCTACT CCTTCTAAAA GCCCAGGATC AACAAGTTCA 
TCCGACACCA CTCCGTCTTC TAACGAAAAT GGGAAAGAGA CAACTCCATT CGAGATGACC 
AAAAATCCAT TCAACGATCT TAAAACCATC CGACCAATAT ATCTAGGTTC AAGTTCTGAC 
GAAGAAGATG ACGAACCGCT AGAGGATATC AGCAAGGCAA CCGATTCCCC ACATTCTTCA 
GAGAAATCTC AGATTCCTGG ATCACCAGGT TCACAGATAA AGAATCCATT GAAACCCTCT 
TCAACGGAAT CGGAAACTAT TGGACCGGAA TCCTCCGAGT CAGAAGACGA CGGAGAGCGA 
AGCACGCCAA CTCCACAAGA GGAGGAAGAG GAAATGATGA GGAACTTCTT CCCAAGACCT 
GTCAGTGCTC AAGCATATCA GCCGCCAGGA CATCAGCCGG AAGATTTTAC GAGTCATATC 
AACGTGAATT TCAAGAGACC AAAAGGCAAA GGAGGATGTG TAACACCGCC GTGCAGAGGA 
GTGGATACTG ATAAGATATC CCTCTACAAG CCAGACCGTG ACAATGAGGA CTCGAGCCCG 
ACACTCGTGA TCAGTAACGC TGCAAAAGAC GATGCATCGC GTGCCGGATA CAAACCAGCT 
CCACCACTGA TCAATCCATA CATCCCTCCA CCACCGCCCC CGCATCCTGA AGAAGAGTAT 
GCCACGCCGT ACACCGTTCC CCCCGGCAAA CAACCATTCC CACCATTTGG AGAAGGACCT 
GCGACTCCGC CAAGAAGCCT GATCCCGGCG CATAATTTCC CTCCTCCAAG TACTTTCCCA 
CCTGATGAGG ATTACCACGA GACTACGACA GATACCGTCC GTCCAACTGC TCAGACTATT 
TCTACTAGAT TTACTGCCCC GACAACTACC ACAACATCAA AGCCCGTTGA ATCCACAACT 
ACCGAGGAAG ATACTCCGGA GCATGTTCCA CCTGCAATTG TTCATATGAC CGCGTCGACG 
CCAAGTGGGA AGAACCCTCC AACCTATATT CCACTGCATC CAGAGACATC CCAGTCCACA 
CCAACTCCAA CAACATCTGC TAGATCCACG AGACCGACAG CTTCAGTCAC AAGACGACCC 
GAGTTCCCAA CTAGAAATAC TCCACCATTT GGAGGAAACG TGCCAAATGG GCCGTCTTCT 
ACGGTACCAC CTGCCAGCAA TCCAACCACT GAAGGATACT TCACACAAGT CTTCACTGAC 
CGAGAGAAGA CAAATTCCGG AGAGTCTGTA GTGACCACCA CAGCTTCATC TACTGATTCT 
GAAACTCCAG GCTCTGGATC ATTCTCGACA TCACCTCAAA CCACTGCTGG ATGGATCTAC 
ACATCTACCG ACGCTACACC AGAATCTGAA TACTCCACTG CATCAAGCTT CCCAACTCTA 
TCAACTTCTG ATCGTTTCAC ACCGTCTGAA TCATCTAGAA GCACGGAGAC CACCACCGTC 
ATCACGCCGT TCACTGAGAC ATTCGAGTCT TCTACACTGA CTCCTGATCA ATTCGAAGAT 
GTGACCTCTA CATTTACAGA TAGATTCAGA CCGTCGGAAT ATGCCACGTC ACCTACACCT 
CCTTCGCCGA CTCGCGTCGT CACCGCAAGC TTCCCGCCGA ACGAGGGATC ATCACCTGGA 
ATCTTGCCAA GCACCAACCC GCCACCCAGA AATAATCCAT ATGCTCCTCC AGAGGGTGCT 
CCAAGGGTAC CAAATCGGTT GATTGGAAAG CCGAGAATCC TCTGTCTGGA GGATGGAATC 
AGTTTCGAGG TTAAAACCAT TTTGCCGCTC ACCGGAGAAG TCTTTGCAAA TGATAGGAAA 
CGGGTTCTAG AATGCCACAA ATCCTTCACT GAAGAGGCCA AGCCAAAAAT GTTCCTGCCA 
TTTTCCACGT GTGGAGTCAA GAATGTTGGT GAACAAGTGG ACAGCAGAGC TCAATACCAC 
ATGCAAGTGG TTCTCATCAT TGATCAAGGA AATGGCACGA ACACTTTGCA ATCATTTATG 
GCTCAATGTG TCCATCAGAA GGTCAATTAT AACAAGCAAG TGCTCCCGAA GAGAATTGAA 
GAGGCTCTGG AAGAACTCCG CTTAGTACCA TCAAAGTTGG AGCAGAAAGC CTCTATGCCG 
AGTGTTCAAA TGCAAATTGT AGTAGACGAG GGACATCACA AGCTTGGCGC TGAGGTCAGT 
GCTGCTGACA TTGGAATGCC ACTGGCTCTT AAATGGAGTA TGGTTCCAGA GTCGGACGCA 
TATGGAATGC ACATACGCAA CTGCAAAGTG GTAGATGCGG TGGGCAAGAT CGATCATACT 
CTAATAGACG AGCAGGGGTG CTCTGCGGAT CTTCAGATCA TCGATCATCC CCATTATGAC 
ACCTACCACG ACACTGCTTC CTCTCACATG TGGGCTTTCA AGGTTCCAGA CATGTCGAGC 
CTCCAGATCA AATGTGATAT TCTGATATGC TCAAACATCA AGTCTTCCGT TACGAACACG 
ACTAGCTGTG AGGATATTCC TTCGCCTCCA TTCTGTGCAG ATGTTGTGAC GTCTCCACCA 
AATTCTATCC TCTCAGATGC TTCTTCATTC ATCAAGCATC GACGTGCTTC AGTACCTTCT 
GAAGTTTCCC CAGCGACGCA ATCGGTGAGA ACCTCGATTT GCTTGTCGAA AAGCTGCAGA 
CCAGATTTTT CAGAAGAAGT CCGAATCTGC GTCAACACTC AATTGGCGAC TACTTCGACA 
GGATTGTCAT TGGCGTTCTT ACTTTTTGCA ATTTCTTTCC AAATTATCGC AAGAACAAAG 
CGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_506848.1
CDS	1..3789
Translation	MRPLLSCFPVIFAVFGPIIAVKPVWKTPVSEPNPNANLEVLKGHSNLAKRAQPNYYTRPEQVSIPEYRPPRQRLPPLPSPKISYQQVPPHQLTSGGSFRPFAWVGPQPKPGSPQTSYYENSPNKGQFPKGLPPLPPNWQQYPVQMIWIPDTPGQTTGAYPYYPGPAGPPVPVSTGSYGLYGPTVTTEKPMEPSVNPETYTGTVAHSPSANPITTTIPYEPPNPYGGQTQVSPSSNSYPDSTVSTTSSNTESYEQPSTSPSERPRSSTRDFENGNGKTVNPTIRPGPQELSGGIGTSSKPRLPNDADYTRDVETGSSEVTTPFGRNTESSTPSKSPGSTSSSDTTPSSNENGKETTPFEMTKNPFNDLKTIRPIYLGSSSDEEDDEPLEDISKATDSPHSSEKSQIPGSPGSQIKNPLKPSSTESETIGPESSESEDDGERSTPTPQEEEEEMMRNFFPRPVSAQAYQPPGHQPEDFTSHINVNFKRPKGKGGCVTPPCRGVDTDKISLYKPDRDNEDSSPTLVISNAAKDDASRAGYKPAPPLINPYIPPPPPPHPEEEYATPYTVPPGKQPFPPFGEGPATPPRSLIPAHNFPPPSTFPPDEDYHETTTDTVRPTAQTISTRFTAPTTTTTSKPVESTTTEEDTPEHVPPAIVHMTASTPSGKNPPTYIPLHPETSQSTPTPTTSARSTRPTASVTRRPEFPTRNTPPFGGNVPNGPSSTVPPASNPTTEGYFTQVFTDREKTNSGESVVTTTASSTDSETPGSGSFSTSPQTTAGWIYTSTDATPESEYSTASSFPTLSTSDRFTPSESSRSTETTTVITPFTETFESSTLTPDQFEDVTSTFTDRFRPSEYATSPTPPSPTRVVTASFPPNEGSSPGILPSTNPPPRNNPYAPPEGAPRVPNRLIGKPRILCLEDGISFEVKTILPLTGEVFANDRKRVLECHKSFTEEAKPKMFLPFSTCGVKNVGEQVDSRAQYHMQVVLIIDQGNGTNTLQSFMAQCVHQKVNYNKQVLPKRIEEALEELRLVPSKLEQKASMPSVQMQIVVDEGHHKLGAEVSAADIGMPLALKWSMVPESDAYGMHIRNCKVVDAVGKIDHTLIDEQGCSADLQIIDHPHYDTYHDTASSHMWAFKVPDMSSLQIKCDILICSNIKSSVTNTTSCEDIPSPPFCADVVTSPPNSILSDASSFIKHRRASVPSEVSPATQSVRTSICLSKSCRPDFSEEVRICVNTQLATTSTGLSLAFLLFAISFQIIARTKRP

Target ORF information:

RefSeq Version	NM_074447.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans regulator of Rme (RME) Circuit formation (rocf-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_074447.3

ORF Insert Sequence:

ATGAGGCCCT TACTCTCATG TTTCCCCGTG ATTTTCGCGG TTTTTGGGCC GATTATTGCG 
GTTAAGCCCG TATGGAAGAC GCCCGTCTCT GAGCCGAATC CAAATGCGAA TCTCGAAGTT 
TTAAAGGGAC ACTCGAATTT GGCGAAGCGT GCTCAACCGA ACTACTACAC ACGTCCCGAG 
CAGGTCTCGA TTCCGGAATA CCGTCCACCT CGTCAACGTC TTCCACCGTT ACCATCTCCA 
AAGATCTCAT ATCAACAAGT TCCTCCACAT CAACTAACAT CTGGTGGAAG CTTCCGACCA 
TTCGCTTGGG TTGGCCCTCA ACCAAAACCC GGCAGCCCTC AAACCAGTTA CTATGAGAAT 
TCTCCAAACA AGGGCCAATT TCCAAAGGGA TTACCTCCAT TGCCACCAAA TTGGCAGCAG 
TATCCAGTGC AGATGATATG GATTCCAGAC ACGCCAGGAC AAACTACTGG AGCGTACCCG 
TATTATCCGG GACCCGCTGG ACCGCCCGTG CCAGTTTCAA CAGGAAGCTA TGGCCTGTAT 
GGACCAACTG TGACAACCGA GAAGCCAATG GAACCGTCGG TCAATCCGGA AACGTATACA 
GGAACCGTCG CTCACAGTCC CTCTGCCAAC CCTATCACCA CTACTATACC TTACGAGCCC 
CCAAATCCAT ATGGAGGACA GACTCAGGTG TCCCCGTCTT CAAATTCCTA TCCGGATTCA 
ACAGTCTCCA CTACTTCGAG CAACACGGAA TCCTATGAAC AGCCATCTAC ATCTCCCTCA 
GAAAGGCCTA GATCATCAAC TCGGGATTTT GAAAATGGAA ATGGAAAAAC TGTAAATCCA 
ACCATCCGCC CAGGACCTCA AGAGCTTAGT GGTGGAATTG GAACTTCTTC CAAGCCTAGA 
CTGCCTAATG ACGCCGACTA CACTAGAGAT GTAGAGACTG GAAGCTCTGA AGTTACAACT 
CCGTTTGGTA GAAACACGGA AAGCTCTACT CCTTCTAAAA GCCCAGGATC AACAAGTTCA 
TCCGACACCA CTCCGTCTTC TAACGAAAAT GGGAAAGAGA CAACTCCATT CGAGATGACC 
AAAAATCCAT TCAACGATCT TAAAACCATC CGACCAATAT ATCTAGGTTC AAGTTCTGAC 
GAAGAAGATG ACGAACCGCT AGAGGATATC AGCAAGGCAA CCGATTCCCC ACATTCTTCA 
GAGAAATCTC AGATTCCTGG ATCACCAGGT TCACAGATAA AGAATCCATT GAAACCCTCT 
TCAACGGAAT CGGAAACTAT TGGACCGGAA TCCTCCGAGT CAGAAGACGA CGGAGAGCGA 
AGCACGCCAA CTCCACAAGA GGAGGAAGAG GAAATGATGA GGAACTTCTT CCCAAGACCT 
GTCAGTGCTC AAGCATATCA GCCGCCAGGA CATCAGCCGG AAGATTTTAC GAGTCATATC 
AACGTGAATT TCAAGAGACC AAAAGGCAAA GGAGGATGTG TAACACCGCC GTGCAGAGGA 
GTGGATACTG ATAAGATATC CCTCTACAAG CCAGACCGTG ACAATGAGGA CTCGAGCCCG 
ACACTCGTGA TCAGTAACGC TGCAAAAGAC GATGCATCGC GTGCCGGATA CAAACCAGCT 
CCACCACTGA TCAATCCATA CATCCCTCCA CCACCGCCCC CGCATCCTGA AGAAGAGTAT 
GCCACGCCGT ACACCGTTCC CCCCGGCAAA CAACCATTCC CACCATTTGG AGAAGGACCT 
GCGACTCCGC CAAGAAGCCT GATCCCGGCG CATAATTTCC CTCCTCCAAG TACTTTCCCA 
CCTGATGAGG ATTACCACGA GACTACGACA GATACCGTCC GTCCAACTGC TCAGACTATT 
TCTACTAGAT TTACTGCCCC GACAACTACC ACAACATCAA AGCCCGTTGA ATCCACAACT 
ACCGAGGAAG ATACTCCGGA GCATGTTCCA CCTGCAATTG TTCATATGAC CGCGTCGACG 
CCAAGTGGGA AGAACCCTCC AACCTATATT CCACTGCATC CAGAGACATC CCAGTCCACA 
CCAACTCCAA CAACATCTGC TAGATCCACG AGACCGACAG CTTCAGTCAC AAGACGACCC 
GAGTTCCCAA CTAGAAATAC TCCACCATTT GGAGGAAACG TGCCAAATGG GCCGTCTTCT 
ACGGTACCAC CTGCCAGCAA TCCAACCACT GAAGGATACT TCACACAAGT CTTCACTGAC 
CGAGAGAAGA CAAATTCCGG AGAGTCTGTA GTGACCACCA CAGCTTCATC TACTGATTCT 
GAAACTCCAG GCTCTGGATC ATTCTCGACA TCACCTCAAA CCACTGCTGG ATGGATCTAC 
ACATCTACCG ACGCTACACC AGAATCTGAA TACTCCACTG CATCAAGCTT CCCAACTCTA 
TCAACTTCTG ATCGTTTCAC ACCGTCTGAA TCATCTAGAA GCACGGAGAC CACCACCGTC 
ATCACGCCGT TCACTGAGAC ATTCGAGTCT TCTACACTGA CTCCTGATCA ATTCGAAGAT 
GTGACCTCTA CATTTACAGA TAGATTCAGA CCGTCGGAAT ATGCCACGTC ACCTACACCT 
CCTTCGCCGA CTCGCGTCGT CACCGCAAGC TTCCCGCCGA ACGAGGGATC ATCACCTGGA 
ATCTTGCCAA GCACCAACCC GCCACCCAGA AATAATCCAT ATGCTCCTCC AGAGGGTGCT 
CCAAGGGTAC CAAATCGGTT GATTGGAAAG CCGAGAATCC TCTGTCTGGA GGATGGAATC 
AGTTTCGAGG TTAAAACCAT TTTGCCGCTC ACCGGAGAAG TCTTTGCAAA TGATAGGAAA 
CGGGTTCTAG AATGCCACAA ATCCTTCACT GAAGAGGCCA AGCCAAAAAT GTTCCTGCCA 
TTTTCCACGT GTGGAGTCAA GAATGTTGGT GAACAAGTGG ACAGCAGAGC TCAATACCAC 
ATGCAAGTGG TTCTCATCAT TGATCAAGGA AATGGCACGA ACACTTTGCA ATCATTTATG 
GCTCAATGTG TCCATCAGAA GGTCAATTAT AACAAGCAAG TGCTCCCGAA GAGAATTGAA 
GAGGCTCTGG AAGAACTCCG CTTAGTACCA TCAAAGTTGG AGCAGAAAGC CTCTATGCCG 
AGTGTTCAAA TGCAAATTGT AGTAGACGAG GGACATCACA AGCTTGGCGC TGAGGTCAGT 
GCTGCTGACA TTGGAATGCC ACTGGCTCTT AAATGGAGTA TGGTTCCAGA GTCGGACGCA 
TATGGAATGC ACATACGCAA CTGCAAAGTG GTAGATGCGG TGGGCAAGAT CGATCATACT 
CTAATAGACG AGCAGGGGTG CTCTGCGGAT CTTCAGATCA TCGATCATCC CCATTATGAC 
ACCTACCACG ACACTGCTTC CTCTCACATG TGGGCTTTCA AGGTTCCAGA CATGTCGAGC 
CTCCAGATCA AATGTGATAT TCTGATATGC TCAAACATCA AGTCTTCCGT TACGAACACG 
ACTAGCTGTG AGGATATTCC TTCGCCTCCA TTCTGTGCAG ATGTTGTGAC GTCTCCACCA 
AATTCTATCC TCTCAGATGC TTCTTCATTC ATCAAGCATC GACGTGCTTC AGTACCTTCT 
GAAGTTTCCC CAGCGACGCA ATCGGTGAGA ACCTCGATTT GCTTGTCGAA AAGCTGCAGA 
CCAGATTTTT CAGAAGAAGT CCGAATCTGC GTCAACACTC AATTGGCGAC TACTTCGACA 
GGATTGTCAT TGGCGTTCTT ACTTTTTGCA ATTTCTTTCC AAATTATCGC AAGAACAAAG 
CGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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rocf-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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