bris-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	bris-1
Entrez Gene ID	177142
Full Name	BRag/Iqsec/Schizo related Arf GEF family member
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001307360.1	NP_001294289.1	BRag/Iqsec/Schizo related Arf GEF family member
NM_001372781.1	NP_001360200.1	SEC7 domain-containing protein
NM_001374891.1	NP_001362058.1	SEC7 domain-containing protein
NM_068019.4	NP_500420.3	BRag/Iqsec/Schizo related Arf GEF family member
NM_001307359.1	NP_001294288.1	BRag/Iqsec/Schizo related Arf GEF family member
NM_001307361.1	NP_001294290.1	BRag/Iqsec/Schizo related Arf GEF family member
NM_068019.5	NP_500420.3	SEC7 domain-containing protein
NM_001307359.2	NP_001294288.1	SEC7 domain-containing protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	siz	NP_730594.1
Caenorhabditis elegans (roundworm)	M02B7.5	NP_500420.3

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bris-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bris-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa344455	NM_001307361.1 Latest version!	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa344455	NM_001374891.1 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCa343870	NM_068019.5 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa344453	NM_001307359.2 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa344454	NM_001372781.1 Latest version!	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OCa344454	NM_001307360.1 Latest version!	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCa343870	NM_068019.4	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCa344453	NM_001307359.1	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bris-1 ( NM_001374891.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001362058.1 Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

Price & Availability↑

CloneID	OCa344455
Clone ID Related Accession (Same CDS sequence)	NM_001374891.1 , NM_001307361.1
Accession Version	NM_001374891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282).

ATGTCACCAC GGCTGGGAAA TCGTCGATTC GGAGCTGCCG CATCACGGTG TGCTCCGGCA 
GCCGCCGAGC TCACGAGCAC AGAAGTCTGG CTTCCGCGCC CGTCGCTTGT CAACCACAAT 
CCGGCACACT CGAACTCGTT ACCGCGGCTC GACAAGCACA GAGTTCATGA ACGCAGCGAG 
TCATCAAACG GCAGGAAAAA ATCGGAGGTA AAGCCGCCAC GACAGATGAC AGATCAGGAG 
AGGAAACGGC AGTACAGGAT AGCCTTGAAC TTTTTCAATA AAAAACCCGA GCGCGGCGTC 
CAACTGCTCA CCGCATGGGG ATTTGTGAAA AATTCGCCGG ATTCCCTTGC CAGCCTACTA 
TTCGGACGTC GTGGACTGAG CAAATCAATG ATTGGAGAAT ATATTGGAAC CCTACACTCA 
CCCTATCATA GTCTAGTTTT AAAATATTTC ACCGCACTAA TCGATATCCG AGGCCTAGAA 
GTGGATGTTG CCCTACGAAA AGCCATGCAA TTCTTCATTC TACCCAAAGA AGCCGAAAAA 
ATCGATCGGA TCATTCAAGC GTTCGCCCTA CACTACTCAA AATCGAATCC GAAGCGAACA 
TCACATTATC GTGGTGGCTG GGATACAATT CATCTACTCT CTTTTGCGAT TATCATGTTA 
AATACTGACC TGCACTCACC GAACGTGAAG CAACGAATGA CTCAGGCGGA TTTTGTGAAA 
AATCTGAGAG GACAGGATAA AATATCAGGG GAGAAAAATG GAGAGGATAT TGATCGAAAA 
ACGCTTGAAG GGATCTATGA TCGGATAAAG AAGGACGAGT TGAAAGCTGG GGATGATCAT 
GTTGCACAAG TTCAAAGAGT TGATCGGGCT ATTGTGGGAA AAGATAAACC GCGCCTCACC 
GAAACGCCCC GTCGGCTCGT CTGTTACTGC CGCCTTCAAC AAGTCTCCGA TCCAACAAAA 
CGTCAATCTT CATCAGCTCG CGAACGTGAC GTCTTCCTAT TCAATGATAT GATCGTCGTG 
GCGAAAGGTG TTCGTCGTGG ATCCACGTCA ATGGCCGGTT GTACGTACAC TCTGAAGCAG 
TGGACGCTTC TTCTCGGAGC ACATGTCACA GAATTCCAGC GAGGACAGTA CGAGTTTGGG 
CTCACAATTA CGTGCCCCAA CAAGGACAAA ATTCACTTTA ACGCTCGCAA TTTCGAGGAT 
CGGTGTCATT TTGTGGCCGA TGTCACCGAA TCGATTCGAG AAGCCACTGA AATGGAGCAA 
GTTCGGCTGG AAATGGAAAT GGAGCAGCAT ACGACTACGA CGATTCGGCC GGATAATCAA 
AGAGATAGTG GACTTCCTGA TATGGATGAA TCTATGAAGC TTTCGAACGG AAGCAACAGC 
AGCGCAGGCT CCTCAAACTC TTCAACAAAC GGCGTAAATA ACTCTTCATT CCGCCGACTT 
TCGTTCAATT CTCTGGATAG TGGAGTCGTA GAAGAGCATC TGGACGCTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362058.1
CDS	421..1914
Translation	MSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_001374891.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374891.1

ORF Insert Sequence:

ATGTCACCAC GGCTGGGAAA TCGTCGATTC GGAGCTGCCG CATCACGGTG TGCTCCGGCA 
GCCGCCGAGC TCACGAGCAC AGAAGTCTGG CTTCCGCGCC CGTCGCTTGT CAACCACAAT 
CCGGCACACT CGAACTCGTT ACCGCGGCTC GACAAGCACA GAGTTCATGA ACGCAGCGAG 
TCATCAAACG GCAGGAAAAA ATCGGAGGTA AAGCCGCCAC GACAGATGAC AGATCAGGAG 
AGGAAACGGC AGTACAGGAT AGCCTTGAAC TTTTTCAATA AAAAACCCGA GCGCGGCGTC 
CAACTGCTCA CCGCATGGGG ATTTGTGAAA AATTCGCCGG ATTCCCTTGC CAGCCTACTA 
TTCGGACGTC GTGGACTGAG CAAATCAATG ATTGGAGAAT ATATTGGAAC CCTACACTCA 
CCCTATCATA GTCTAGTTTT AAAATATTTC ACCGCACTAA TCGATATCCG AGGCCTAGAA 
GTGGATGTTG CCCTACGAAA AGCCATGCAA TTCTTCATTC TACCCAAAGA AGCCGAAAAA 
ATCGATCGGA TCATTCAAGC GTTCGCCCTA CACTACTCAA AATCGAATCC GAAGCGAACA 
TCACATTATC GTGGTGGCTG GGATACAATT CATCTACTCT CTTTTGCGAT TATCATGTTA 
AATACTGACC TGCACTCACC GAACGTGAAG CAACGAATGA CTCAGGCGGA TTTTGTGAAA 
AATCTGAGAG GACAGGATAA AATATCAGGG GAGAAAAATG GAGAGGATAT TGATCGAAAA 
ACGCTTGAAG GGATCTATGA TCGGATAAAG AAGGACGAGT TGAAAGCTGG GGATGATCAT 
GTTGCACAAG TTCAAAGAGT TGATCGGGCT ATTGTGGGAA AAGATAAACC GCGCCTCACC 
GAAACGCCCC GTCGGCTCGT CTGTTACTGC CGCCTTCAAC AAGTCTCCGA TCCAACAAAA 
CGTCAATCTT CATCAGCTCG CGAACGTGAC GTCTTCCTAT TCAATGATAT GATCGTCGTG 
GCGAAAGGTG TTCGTCGTGG ATCCACGTCA ATGGCCGGTT GTACGTACAC TCTGAAGCAG 
TGGACGCTTC TTCTCGGAGC ACATGTCACA GAATTCCAGC GAGGACAGTA CGAGTTTGGG 
CTCACAATTA CGTGCCCCAA CAAGGACAAA ATTCACTTTA ACGCTCGCAA TTTCGAGGAT 
CGGTGTCATT TTGTGGCCGA TGTCACCGAA TCGATTCGAG AAGCCACTGA AATGGAGCAA 
GTTCGGCTGG AAATGGAAAT GGAGCAGCAT ACGACTACGA CGATTCGGCC GGATAATCAA 
AGAGATAGTG GACTTCCTGA TATGGATGAA TCTATGAAGC TTTCGAACGG AAGCAACAGC 
AGCGCAGGCT CCTCAAACTC TTCAACAAAC GGCGTAAATA ACTCTTCATT CCGCCGACTT 
TCGTTCAATT CTCTGGATAG TGGAGTCGTA GAAGAGCATC TGGACGCTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_001307361.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294290.1 Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

Price & Availability↑

CloneID	OCa344455
Clone ID Related Accession (Same CDS sequence)	NM_001374891.1 , NM_001307361.1
Accession Version	NM_001307361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	BRag/Iqsec/Schizo related Arf GEF family member
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTCACCAC GGCTGGGAAA TCGTCGATTC GGAGCTGCCG CATCACGGTG TGCTCCGGCA 
GCCGCCGAGC TCACGAGCAC AGAAGTCTGG CTTCCGCGCC CGTCGCTTGT CAACCACAAT 
CCGGCACACT CGAACTCGTT ACCGCGGCTC GACAAGCACA GAGTTCATGA ACGCAGCGAG 
TCATCAAACG GCAGGAAAAA ATCGGAGGTA AAGCCGCCAC GACAGATGAC AGATCAGGAG 
AGGAAACGGC AGTACAGGAT AGCCTTGAAC TTTTTCAATA AAAAACCCGA GCGCGGCGTC 
CAACTGCTCA CCGCATGGGG ATTTGTGAAA AATTCGCCGG ATTCCCTTGC CAGCCTACTA 
TTCGGACGTC GTGGACTGAG CAAATCAATG ATTGGAGAAT ATATTGGAAC CCTACACTCA 
CCCTATCATA GTCTAGTTTT AAAATATTTC ACCGCACTAA TCGATATCCG AGGCCTAGAA 
GTGGATGTTG CCCTACGAAA AGCCATGCAA TTCTTCATTC TACCCAAAGA AGCCGAAAAA 
ATCGATCGGA TCATTCAAGC GTTCGCCCTA CACTACTCAA AATCGAATCC GAAGCGAACA 
TCACATTATC GTGGTGGCTG GGATACAATT CATCTACTCT CTTTTGCGAT TATCATGTTA 
AATACTGACC TGCACTCACC GAACGTGAAG CAACGAATGA CTCAGGCGGA TTTTGTGAAA 
AATCTGAGAG GACAGGATAA AATATCAGGG GAGAAAAATG GAGAGGATAT TGATCGAAAA 
ACGCTTGAAG GGATCTATGA TCGGATAAAG AAGGACGAGT TGAAAGCTGG GGATGATCAT 
GTTGCACAAG TTCAAAGAGT TGATCGGGCT ATTGTGGGAA AAGATAAACC GCGCCTCACC 
GAAACGCCCC GTCGGCTCGT CTGTTACTGC CGCCTTCAAC AAGTCTCCGA TCCAACAAAA 
CGTCAATCTT CATCAGCTCG CGAACGTGAC GTCTTCCTAT TCAATGATAT GATCGTCGTG 
GCGAAAGGTG TTCGTCGTGG ATCCACGTCA ATGGCCGGTT GTACGTACAC TCTGAAGCAG 
TGGACGCTTC TTCTCGGAGC ACATGTCACA GAATTCCAGC GAGGACAGTA CGAGTTTGGG 
CTCACAATTA CGTGCCCCAA CAAGGACAAA ATTCACTTTA ACGCTCGCAA TTTCGAGGAT 
CGGTGTCATT TTGTGGCCGA TGTCACCGAA TCGATTCGAG AAGCCACTGA AATGGAGCAA 
GTTCGGCTGG AAATGGAAAT GGAGCAGCAT ACGACTACGA CGATTCGGCC GGATAATCAA 
AGAGATAGTG GACTTCCTGA TATGGATGAA TCTATGAAGC TTTCGAACGG AAGCAACAGC 
AGCGCAGGCT CCTCAAACTC TTCAACAAAC GGCGTAAATA ACTCTTCATT CCGCCGACTT 
TCGTTCAATT CTCTGGATAG TGGAGTCGTA GAAGAGCATC TGGACGCTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294290.1
CDS	1..1494
Translation	MSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_001307361.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307361.1

ORF Insert Sequence:

ATGTCACCAC GGCTGGGAAA TCGTCGATTC GGAGCTGCCG CATCACGGTG TGCTCCGGCA 
GCCGCCGAGC TCACGAGCAC AGAAGTCTGG CTTCCGCGCC CGTCGCTTGT CAACCACAAT 
CCGGCACACT CGAACTCGTT ACCGCGGCTC GACAAGCACA GAGTTCATGA ACGCAGCGAG 
TCATCAAACG GCAGGAAAAA ATCGGAGGTA AAGCCGCCAC GACAGATGAC AGATCAGGAG 
AGGAAACGGC AGTACAGGAT AGCCTTGAAC TTTTTCAATA AAAAACCCGA GCGCGGCGTC 
CAACTGCTCA CCGCATGGGG ATTTGTGAAA AATTCGCCGG ATTCCCTTGC CAGCCTACTA 
TTCGGACGTC GTGGACTGAG CAAATCAATG ATTGGAGAAT ATATTGGAAC CCTACACTCA 
CCCTATCATA GTCTAGTTTT AAAATATTTC ACCGCACTAA TCGATATCCG AGGCCTAGAA 
GTGGATGTTG CCCTACGAAA AGCCATGCAA TTCTTCATTC TACCCAAAGA AGCCGAAAAA 
ATCGATCGGA TCATTCAAGC GTTCGCCCTA CACTACTCAA AATCGAATCC GAAGCGAACA 
TCACATTATC GTGGTGGCTG GGATACAATT CATCTACTCT CTTTTGCGAT TATCATGTTA 
AATACTGACC TGCACTCACC GAACGTGAAG CAACGAATGA CTCAGGCGGA TTTTGTGAAA 
AATCTGAGAG GACAGGATAA AATATCAGGG GAGAAAAATG GAGAGGATAT TGATCGAAAA 
ACGCTTGAAG GGATCTATGA TCGGATAAAG AAGGACGAGT TGAAAGCTGG GGATGATCAT 
GTTGCACAAG TTCAAAGAGT TGATCGGGCT ATTGTGGGAA AAGATAAACC GCGCCTCACC 
GAAACGCCCC GTCGGCTCGT CTGTTACTGC CGCCTTCAAC AAGTCTCCGA TCCAACAAAA 
CGTCAATCTT CATCAGCTCG CGAACGTGAC GTCTTCCTAT TCAATGATAT GATCGTCGTG 
GCGAAAGGTG TTCGTCGTGG ATCCACGTCA ATGGCCGGTT GTACGTACAC TCTGAAGCAG 
TGGACGCTTC TTCTCGGAGC ACATGTCACA GAATTCCAGC GAGGACAGTA CGAGTTTGGG 
CTCACAATTA CGTGCCCCAA CAAGGACAAA ATTCACTTTA ACGCTCGCAA TTTCGAGGAT 
CGGTGTCATT TTGTGGCCGA TGTCACCGAA TCGATTCGAG AAGCCACTGA AATGGAGCAA 
GTTCGGCTGG AAATGGAAAT GGAGCAGCAT ACGACTACGA CGATTCGGCC GGATAATCAA 
AGAGATAGTG GACTTCCTGA TATGGATGAA TCTATGAAGC TTTCGAACGG AAGCAACAGC 
AGCGCAGGCT CCTCAAACTC TTCAACAAAC GGCGTAAATA ACTCTTCATT CCGCCGACTT 
TCGTTCAATT CTCTGGATAG TGGAGTCGTA GAAGAGCATC TGGACGCTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_068019.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_500420.3 Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

Price & Availability↑

CloneID	OCa343870
Clone ID Related Accession (Same CDS sequence)	NM_068019.4 , NM_068019.5
Accession Version	NM_068019.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	BRag/Iqsec/Schizo related Arf GEF family member
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_068019.3. COMPLETENESS: incomplete on both ends.

ATGCGAGAAA CAAAAGAATG GCGAGGCACG ACGTCGACGA GCCGTGTCTT CGTACAGAAG 
GGCTATCAAT TGTCCGATGA GCTGGCTCTT CGAAAAGTGC AAGTGCTCGA GCGAAGATAT 
GGTGGAATGC AACTGGCTCA CAGAGCAGCA ACGGTTATAC AACGCGCGTG GCGCGAGTAC 
TGTATTATGA AGCAGTGGAT GGTGATTCGA CATAAACATG ATACAATACC AGACCACCTC 
GATGCAAGAT GCGGAGTGTC AAACAGTCAG CGGAACGAGC TATCACTAAC CAGCCGATAC 
GCCACAAGTC TCAGCGCACA GCTCCGGATA GACCGTGCCG CTCGTTCAAC AACACTGGAC 
AGAAGTCCGC CGTCACCGGC GCCGCCCGTC TTGGTCAATT TGCAGAATTC GAATATGATT 
GACAGTCTAA TGTCACCACG GCTGGGAAAT CGTCGATTCG GAGCTGCCGC ATCACGGTGT 
GCTCCGGCAG CCGCCGAGCT CACGAGCACA GAAGTCTGGC TTCCGCGCCC GTCGCTTGTC 
AACCACAATC CGGCACACTC GAACTCGTTA CCGCGGCTCG ACAAGCACAG AGTTCATGAA 
CGCAGCGAGT CATCAAACGG CAGGAAAAAA TCGGAGGTAA AGCCGCCACG ACAGATGACA 
GATCAGGAGA GGAAACGGCA GTACAGGATA GCCTTGAACT TTTTCAATAA AAAACCCGAG 
CGCGGCGTCC AACTGCTCAC CGCATGGGGA TTTGTGAAAA ATTCGCCGGA TTCCCTTGCC 
AGCCTACTAT TCGGACGTCG TGGACTGAGC AAATCAATGA TTGGAGAATA TATTGGAACC 
CTACACTCAC CCTATCATAG TCTAGTTTTA AAATATTTCA CCGCACTAAT CGATATCCGA 
GGCCTAGAAG TGGATGTTGC CCTACGAAAA GCCATGCAAT TCTTCATTCT ACCCAAAGAA 
GCCGAAAAAA TCGATCGGAT CATTCAAGCG TTCGCCCTAC ACTACTCAAA ATCGAATCCG 
AAGCGAACAT CACATTATCG TGGTGGCTGG GATACAATTC ATCTACTCTC TTTTGCGATT 
ATCATGTTAA ATACTGACCT GCACTCACCG AACGTGAAGC AACGAATGAC TCAGGCGGAT 
TTTGTGAAAA ATCTGAGAGG ACAGGATAAA ATATCAGGGG AGAAAAATGG AGAGGATATT 
GATCGAAAAA CGCTTGAAGG GATCTATGAT CGGATAAAGA AGGACGAGTT GAAAGCTGGG 
GATGATCATG TTGCACAAGT TCAAAGAGTT GATCGGGCTA TTGTGGGAAA AGATAAACCG 
CGCCTCACCG AAACGCCCCG TCGGCTCGTC TGTTACTGCC GCCTTCAACA AGTCTCCGAT 
CCAACAAAAC GTCAATCTTC ATCAGCTCGC GAACGTGACG TCTTCCTATT CAATGATATG 
ATCGTCGTGG CGAAAGGTGT TCGTCGTGGA TCCACGTCAA TGGCCGGTTG TACGTACACT 
CTGAAGCAGT GGACGCTTCT TCTCGGAGCA CATGTCACAG AATTCCAGCG AGGACAGTAC 
GAGTTTGGGC TCACAATTAC GTGCCCCAAC AAGGACAAAA TTCACTTTAA CGCTCGCAAT 
TTCGAGGATC GGTGTCATTT TGTGGCCGAT GTCACCGAAT CGATTCGAGA AGCCACTGAA 
ATGGAGCAAG TTCGGCTGGA AATGGAAATG GAGCAGCATA CGACTACGAC GATTCGGCCG 
GATAATCAAA GAGATAGTGG ACTTCCTGAT ATGGATGAAT CTATGAAGCT TTCGAACGGA 
AGCAACAGCA GCGCAGGCTC CTCAAACTCT TCAACAAACG GCGTAAATAA CTCTTCATTC 
CGCCGACTTT CGTTCAATTC TCTGGATAGT GGAGTCGTAG AAGAGCATCT GGACGCTTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_500420.3
CDS	1..1923
Translation	MRETKEWRGTTSTSRVFVQKGYQLSDELALRKVQVLERRYGGMQLAHRAATVIQRAWREYCIMKQWMVIRHKHDTIPDHLDARCGVSNSQRNELSLTSRYATSLSAQLRIDRAARSTTLDRSPPSPAPPVLVNLQNSNMIDSLMSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_068019.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068019.4

ORF Insert Sequence:

ATGCGAGAAA CAAAAGAATG GCGAGGCACG ACGTCGACGA GCCGTGTCTT CGTACAGAAG 
GGCTATCAAT TGTCCGATGA GCTGGCTCTT CGAAAAGTGC AAGTGCTCGA GCGAAGATAT 
GGTGGAATGC AACTGGCTCA CAGAGCAGCA ACGGTTATAC AACGCGCGTG GCGCGAGTAC 
TGTATTATGA AGCAGTGGAT GGTGATTCGA CATAAACATG ATACAATACC AGACCACCTC 
GATGCAAGAT GCGGAGTGTC AAACAGTCAG CGGAACGAGC TATCACTAAC CAGCCGATAC 
GCCACAAGTC TCAGCGCACA GCTCCGGATA GACCGTGCCG CTCGTTCAAC AACACTGGAC 
AGAAGTCCGC CGTCACCGGC GCCGCCCGTC TTGGTCAATT TGCAGAATTC GAATATGATT 
GACAGTCTAA TGTCACCACG GCTGGGAAAT CGTCGATTCG GAGCTGCCGC ATCACGGTGT 
GCTCCGGCAG CCGCCGAGCT CACGAGCACA GAAGTCTGGC TTCCGCGCCC GTCGCTTGTC 
AACCACAATC CGGCACACTC GAACTCGTTA CCGCGGCTCG ACAAGCACAG AGTTCATGAA 
CGCAGCGAGT CATCAAACGG CAGGAAAAAA TCGGAGGTAA AGCCGCCACG ACAGATGACA 
GATCAGGAGA GGAAACGGCA GTACAGGATA GCCTTGAACT TTTTCAATAA AAAACCCGAG 
CGCGGCGTCC AACTGCTCAC CGCATGGGGA TTTGTGAAAA ATTCGCCGGA TTCCCTTGCC 
AGCCTACTAT TCGGACGTCG TGGACTGAGC AAATCAATGA TTGGAGAATA TATTGGAACC 
CTACACTCAC CCTATCATAG TCTAGTTTTA AAATATTTCA CCGCACTAAT CGATATCCGA 
GGCCTAGAAG TGGATGTTGC CCTACGAAAA GCCATGCAAT TCTTCATTCT ACCCAAAGAA 
GCCGAAAAAA TCGATCGGAT CATTCAAGCG TTCGCCCTAC ACTACTCAAA ATCGAATCCG 
AAGCGAACAT CACATTATCG TGGTGGCTGG GATACAATTC ATCTACTCTC TTTTGCGATT 
ATCATGTTAA ATACTGACCT GCACTCACCG AACGTGAAGC AACGAATGAC TCAGGCGGAT 
TTTGTGAAAA ATCTGAGAGG ACAGGATAAA ATATCAGGGG AGAAAAATGG AGAGGATATT 
GATCGAAAAA CGCTTGAAGG GATCTATGAT CGGATAAAGA AGGACGAGTT GAAAGCTGGG 
GATGATCATG TTGCACAAGT TCAAAGAGTT GATCGGGCTA TTGTGGGAAA AGATAAACCG 
CGCCTCACCG AAACGCCCCG TCGGCTCGTC TGTTACTGCC GCCTTCAACA AGTCTCCGAT 
CCAACAAAAC GTCAATCTTC ATCAGCTCGC GAACGTGACG TCTTCCTATT CAATGATATG 
ATCGTCGTGG CGAAAGGTGT TCGTCGTGGA TCCACGTCAA TGGCCGGTTG TACGTACACT 
CTGAAGCAGT GGACGCTTCT TCTCGGAGCA CATGTCACAG AATTCCAGCG AGGACAGTAC 
GAGTTTGGGC TCACAATTAC GTGCCCCAAC AAGGACAAAA TTCACTTTAA CGCTCGCAAT 
TTCGAGGATC GGTGTCATTT TGTGGCCGAT GTCACCGAAT CGATTCGAGA AGCCACTGAA 
ATGGAGCAAG TTCGGCTGGA AATGGAAATG GAGCAGCATA CGACTACGAC GATTCGGCCG 
GATAATCAAA GAGATAGTGG ACTTCCTGAT ATGGATGAAT CTATGAAGCT TTCGAACGGA 
AGCAACAGCA GCGCAGGCTC CTCAAACTCT TCAACAAACG GCGTAAATAA CTCTTCATTC 
CGCCGACTTT CGTTCAATTC TCTGGATAGT GGAGTCGTAG AAGAGCATCT GGACGCTTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_068019.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_500420.3 Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

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CloneID	OCa343870
Clone ID Related Accession (Same CDS sequence)	NM_068019.4 , NM_068019.5
Accession Version	NM_068019.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_068019.4.

ATGCGAGAAA CAAAAGAATG GCGAGGCACG ACGTCGACGA GCCGTGTCTT CGTACAGAAG 
GGCTATCAAT TGTCCGATGA GCTGGCTCTT CGAAAAGTGC AAGTGCTCGA GCGAAGATAT 
GGTGGAATGC AACTGGCTCA CAGAGCAGCA ACGGTTATAC AACGCGCGTG GCGCGAGTAC 
TGTATTATGA AGCAGTGGAT GGTGATTCGA CATAAACATG ATACAATACC AGACCACCTC 
GATGCAAGAT GCGGAGTGTC AAACAGTCAG CGGAACGAGC TATCACTAAC CAGCCGATAC 
GCCACAAGTC TCAGCGCACA GCTCCGGATA GACCGTGCCG CTCGTTCAAC AACACTGGAC 
AGAAGTCCGC CGTCACCGGC GCCGCCCGTC TTGGTCAATT TGCAGAATTC GAATATGATT 
GACAGTCTAA TGTCACCACG GCTGGGAAAT CGTCGATTCG GAGCTGCCGC ATCACGGTGT 
GCTCCGGCAG CCGCCGAGCT CACGAGCACA GAAGTCTGGC TTCCGCGCCC GTCGCTTGTC 
AACCACAATC CGGCACACTC GAACTCGTTA CCGCGGCTCG ACAAGCACAG AGTTCATGAA 
CGCAGCGAGT CATCAAACGG CAGGAAAAAA TCGGAGGTAA AGCCGCCACG ACAGATGACA 
GATCAGGAGA GGAAACGGCA GTACAGGATA GCCTTGAACT TTTTCAATAA AAAACCCGAG 
CGCGGCGTCC AACTGCTCAC CGCATGGGGA TTTGTGAAAA ATTCGCCGGA TTCCCTTGCC 
AGCCTACTAT TCGGACGTCG TGGACTGAGC AAATCAATGA TTGGAGAATA TATTGGAACC 
CTACACTCAC CCTATCATAG TCTAGTTTTA AAATATTTCA CCGCACTAAT CGATATCCGA 
GGCCTAGAAG TGGATGTTGC CCTACGAAAA GCCATGCAAT TCTTCATTCT ACCCAAAGAA 
GCCGAAAAAA TCGATCGGAT CATTCAAGCG TTCGCCCTAC ACTACTCAAA ATCGAATCCG 
AAGCGAACAT CACATTATCG TGGTGGCTGG GATACAATTC ATCTACTCTC TTTTGCGATT 
ATCATGTTAA ATACTGACCT GCACTCACCG AACGTGAAGC AACGAATGAC TCAGGCGGAT 
TTTGTGAAAA ATCTGAGAGG ACAGGATAAA ATATCAGGGG AGAAAAATGG AGAGGATATT 
GATCGAAAAA CGCTTGAAGG GATCTATGAT CGGATAAAGA AGGACGAGTT GAAAGCTGGG 
GATGATCATG TTGCACAAGT TCAAAGAGTT GATCGGGCTA TTGTGGGAAA AGATAAACCG 
CGCCTCACCG AAACGCCCCG TCGGCTCGTC TGTTACTGCC GCCTTCAACA AGTCTCCGAT 
CCAACAAAAC GTCAATCTTC ATCAGCTCGC GAACGTGACG TCTTCCTATT CAATGATATG 
ATCGTCGTGG CGAAAGGTGT TCGTCGTGGA TCCACGTCAA TGGCCGGTTG TACGTACACT 
CTGAAGCAGT GGACGCTTCT TCTCGGAGCA CATGTCACAG AATTCCAGCG AGGACAGTAC 
GAGTTTGGGC TCACAATTAC GTGCCCCAAC AAGGACAAAA TTCACTTTAA CGCTCGCAAT 
TTCGAGGATC GGTGTCATTT TGTGGCCGAT GTCACCGAAT CGATTCGAGA AGCCACTGAA 
ATGGAGCAAG TTCGGCTGGA AATGGAAATG GAGCAGCATA CGACTACGAC GATTCGGCCG 
GATAATCAAA GAGATAGTGG ACTTCCTGAT ATGGATGAAT CTATGAAGCT TTCGAACGGA 
AGCAACAGCA GCGCAGGCTC CTCAAACTCT TCAACAAACG GCGTAAATAA CTCTTCATTC 
CGCCGACTTT CGTTCAATTC TCTGGATAGT GGAGTCGTAG AAGAGCATCT GGACGCTTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_500420.3
CDS	6..1928
Translation	MRETKEWRGTTSTSRVFVQKGYQLSDELALRKVQVLERRYGGMQLAHRAATVIQRAWREYCIMKQWMVIRHKHDTIPDHLDARCGVSNSQRNELSLTSRYATSLSAQLRIDRAARSTTLDRSPPSPAPPVLVNLQNSNMIDSLMSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_068019.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068019.5

ORF Insert Sequence:

ATGCGAGAAA CAAAAGAATG GCGAGGCACG ACGTCGACGA GCCGTGTCTT CGTACAGAAG 
GGCTATCAAT TGTCCGATGA GCTGGCTCTT CGAAAAGTGC AAGTGCTCGA GCGAAGATAT 
GGTGGAATGC AACTGGCTCA CAGAGCAGCA ACGGTTATAC AACGCGCGTG GCGCGAGTAC 
TGTATTATGA AGCAGTGGAT GGTGATTCGA CATAAACATG ATACAATACC AGACCACCTC 
GATGCAAGAT GCGGAGTGTC AAACAGTCAG CGGAACGAGC TATCACTAAC CAGCCGATAC 
GCCACAAGTC TCAGCGCACA GCTCCGGATA GACCGTGCCG CTCGTTCAAC AACACTGGAC 
AGAAGTCCGC CGTCACCGGC GCCGCCCGTC TTGGTCAATT TGCAGAATTC GAATATGATT 
GACAGTCTAA TGTCACCACG GCTGGGAAAT CGTCGATTCG GAGCTGCCGC ATCACGGTGT 
GCTCCGGCAG CCGCCGAGCT CACGAGCACA GAAGTCTGGC TTCCGCGCCC GTCGCTTGTC 
AACCACAATC CGGCACACTC GAACTCGTTA CCGCGGCTCG ACAAGCACAG AGTTCATGAA 
CGCAGCGAGT CATCAAACGG CAGGAAAAAA TCGGAGGTAA AGCCGCCACG ACAGATGACA 
GATCAGGAGA GGAAACGGCA GTACAGGATA GCCTTGAACT TTTTCAATAA AAAACCCGAG 
CGCGGCGTCC AACTGCTCAC CGCATGGGGA TTTGTGAAAA ATTCGCCGGA TTCCCTTGCC 
AGCCTACTAT TCGGACGTCG TGGACTGAGC AAATCAATGA TTGGAGAATA TATTGGAACC 
CTACACTCAC CCTATCATAG TCTAGTTTTA AAATATTTCA CCGCACTAAT CGATATCCGA 
GGCCTAGAAG TGGATGTTGC CCTACGAAAA GCCATGCAAT TCTTCATTCT ACCCAAAGAA 
GCCGAAAAAA TCGATCGGAT CATTCAAGCG TTCGCCCTAC ACTACTCAAA ATCGAATCCG 
AAGCGAACAT CACATTATCG TGGTGGCTGG GATACAATTC ATCTACTCTC TTTTGCGATT 
ATCATGTTAA ATACTGACCT GCACTCACCG AACGTGAAGC AACGAATGAC TCAGGCGGAT 
TTTGTGAAAA ATCTGAGAGG ACAGGATAAA ATATCAGGGG AGAAAAATGG AGAGGATATT 
GATCGAAAAA CGCTTGAAGG GATCTATGAT CGGATAAAGA AGGACGAGTT GAAAGCTGGG 
GATGATCATG TTGCACAAGT TCAAAGAGTT GATCGGGCTA TTGTGGGAAA AGATAAACCG 
CGCCTCACCG AAACGCCCCG TCGGCTCGTC TGTTACTGCC GCCTTCAACA AGTCTCCGAT 
CCAACAAAAC GTCAATCTTC ATCAGCTCGC GAACGTGACG TCTTCCTATT CAATGATATG 
ATCGTCGTGG CGAAAGGTGT TCGTCGTGGA TCCACGTCAA TGGCCGGTTG TACGTACACT 
CTGAAGCAGT GGACGCTTCT TCTCGGAGCA CATGTCACAG AATTCCAGCG AGGACAGTAC 
GAGTTTGGGC TCACAATTAC GTGCCCCAAC AAGGACAAAA TTCACTTTAA CGCTCGCAAT 
TTCGAGGATC GGTGTCATTT TGTGGCCGAT GTCACCGAAT CGATTCGAGA AGCCACTGAA 
ATGGAGCAAG TTCGGCTGGA AATGGAAATG GAGCAGCATA CGACTACGAC GATTCGGCCG 
GATAATCAAA GAGATAGTGG ACTTCCTGAT ATGGATGAAT CTATGAAGCT TTCGAACGGA 
AGCAACAGCA GCGCAGGCTC CTCAAACTCT TCAACAAACG GCGTAAATAA CTCTTCATTC 
CGCCGACTTT CGTTCAATTC TCTGGATAGT GGAGTCGTAG AAGAGCATCT GGACGCTTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_001307359.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294288.1 Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

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CloneID	OCa344453
Clone ID Related Accession (Same CDS sequence)	NM_001307359.1 , NM_001307359.2
Accession Version	NM_001307359.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	BRag/Iqsec/Schizo related Arf GEF family member
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGAATTTAT TTTACGCAAT TCTCGCTATA ATCTCAAAGA TTTTTAACGC ATTAATCCGA 
TACGCCACAA GTCTCAGCGC ACAGCTCCGG ATAGACCGTG CCGCTCGTTC AACAACACTG 
GACAGAAGTC CGCCGTCACC GGCGCCGCCC GTCTTGGTCA ATTTGCAGAA TTCGAATATG 
ATTGACAGTC TAATGTCACC ACGGCTGGGA AATCGTCGAT TCGGAGCTGC CGCATCACGG 
TGTGCTCCGG CAGCCGCCGA GCTCACGAGC ACAGAAGTCT GGCTTCCGCG CCCGTCGCTT 
GTCAACCACA ATCCGGCACA CTCGAACTCG TTACCGCGGC TCGACAAGCA CAGAGTTCAT 
GAACGCAGCG AGTCATCAAA CGGCAGGAAA AAATCGGAGG TAAAGCCGCC ACGACAGATG 
ACAGATCAGG AGAGGAAACG GCAGTACAGG ATAGCCTTGA ACTTTTTCAA TAAAAAACCC 
GAGCGCGGCG TCCAACTGCT CACCGCATGG GGATTTGTGA AAAATTCGCC GGATTCCCTT 
GCCAGCCTAC TATTCGGACG TCGTGGACTG AGCAAATCAA TGATTGGAGA ATATATTGGA 
ACCCTACACT CACCCTATCA TAGTCTAGTT TTAAAATATT TCACCGCACT AATCGATATC 
CGAGGCCTAG AAGTGGATGT TGCCCTACGA AAAGCCATGC AATTCTTCAT TCTACCCAAA 
GAAGCCGAAA AAATCGATCG GATCATTCAA GCGTTCGCCC TACACTACTC AAAATCGAAT 
CCGAAGCGAA CATCACATTA TCGTGGTGGC TGGGATACAA TTCATCTACT CTCTTTTGCG 
ATTATCATGT TAAATACTGA CCTGCACTCA CCGAACGTGA AGCAACGAAT GACTCAGGCG 
GATTTTGTGA AAAATCTGAG AGGACAGGAT AAAATATCAG GGGAGAAAAA TGGAGAGGAT 
ATTGATCGAA AAACGCTTGA AGGGATCTAT GATCGGATAA AGAAGGACGA GTTGAAAGCT 
GGGGATGATC ATGTTGCACA AGTTCAAAGA GTTGATCGGG CTATTGTGGG AAAAGATAAA 
CCGCGCCTCA CCGAAACGCC CCGTCGGCTC GTCTGTTACT GCCGCCTTCA ACAAGTCTCC 
GATCCAACAA AACGTCAATC TTCATCAGCT CGCGAACGTG ACGTCTTCCT ATTCAATGAT 
ATGATCGTCG TGGCGAAAGG TGTTCGTCGT GGATCCACGT CAATGGCCGG TTGTACGTAC 
ACTCTGAAGC AGTGGACGCT TCTTCTCGGA GCACATGTCA CAGAATTCCA GCGAGGACAG 
TACGAGTTTG GGCTCACAAT TACGTGCCCC AACAAGGACA AAATTCACTT TAACGCTCGC 
AATTTCGAGG ATCGGTGTCA TTTTGTGGCC GATGTCACCG AATCGATTCG AGAAGCCACT 
GAAATGGAGC AAGTTCGGCT GGAAATGGAA ATGGAGCAGC ATACGACTAC GACGATTCGG 
CCGGATAATC AAAGAGATAG TGGACTTCCT GATATGGATG AATCTATGAA GCTTTCGAAC 
GGAAGCAACA GCAGCGCAGG CTCCTCAAAC TCTTCAACAA ACGGCGTAAA TAACTCTTCA 
TTCCGCCGAC TTTCGTTCAA TTCTCTGGAT AGTGGAGTCG TAGAAGAGCA TCTGGACGCT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294288.1
CDS	1..1686
Translation	MNLFYAILAIISKIFNALIRYATSLSAQLRIDRAARSTTLDRSPPSPAPPVLVNLQNSNMIDSLMSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_001307359.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307359.1

ORF Insert Sequence:

ATGAATTTAT TTTACGCAAT TCTCGCTATA ATCTCAAAGA TTTTTAACGC ATTAATCCGA 
TACGCCACAA GTCTCAGCGC ACAGCTCCGG ATAGACCGTG CCGCTCGTTC AACAACACTG 
GACAGAAGTC CGCCGTCACC GGCGCCGCCC GTCTTGGTCA ATTTGCAGAA TTCGAATATG 
ATTGACAGTC TAATGTCACC ACGGCTGGGA AATCGTCGAT TCGGAGCTGC CGCATCACGG 
TGTGCTCCGG CAGCCGCCGA GCTCACGAGC ACAGAAGTCT GGCTTCCGCG CCCGTCGCTT 
GTCAACCACA ATCCGGCACA CTCGAACTCG TTACCGCGGC TCGACAAGCA CAGAGTTCAT 
GAACGCAGCG AGTCATCAAA CGGCAGGAAA AAATCGGAGG TAAAGCCGCC ACGACAGATG 
ACAGATCAGG AGAGGAAACG GCAGTACAGG ATAGCCTTGA ACTTTTTCAA TAAAAAACCC 
GAGCGCGGCG TCCAACTGCT CACCGCATGG GGATTTGTGA AAAATTCGCC GGATTCCCTT 
GCCAGCCTAC TATTCGGACG TCGTGGACTG AGCAAATCAA TGATTGGAGA ATATATTGGA 
ACCCTACACT CACCCTATCA TAGTCTAGTT TTAAAATATT TCACCGCACT AATCGATATC 
CGAGGCCTAG AAGTGGATGT TGCCCTACGA AAAGCCATGC AATTCTTCAT TCTACCCAAA 
GAAGCCGAAA AAATCGATCG GATCATTCAA GCGTTCGCCC TACACTACTC AAAATCGAAT 
CCGAAGCGAA CATCACATTA TCGTGGTGGC TGGGATACAA TTCATCTACT CTCTTTTGCG 
ATTATCATGT TAAATACTGA CCTGCACTCA CCGAACGTGA AGCAACGAAT GACTCAGGCG 
GATTTTGTGA AAAATCTGAG AGGACAGGAT AAAATATCAG GGGAGAAAAA TGGAGAGGAT 
ATTGATCGAA AAACGCTTGA AGGGATCTAT GATCGGATAA AGAAGGACGA GTTGAAAGCT 
GGGGATGATC ATGTTGCACA AGTTCAAAGA GTTGATCGGG CTATTGTGGG AAAAGATAAA 
CCGCGCCTCA CCGAAACGCC CCGTCGGCTC GTCTGTTACT GCCGCCTTCA ACAAGTCTCC 
GATCCAACAA AACGTCAATC TTCATCAGCT CGCGAACGTG ACGTCTTCCT ATTCAATGAT 
ATGATCGTCG TGGCGAAAGG TGTTCGTCGT GGATCCACGT CAATGGCCGG TTGTACGTAC 
ACTCTGAAGC AGTGGACGCT TCTTCTCGGA GCACATGTCA CAGAATTCCA GCGAGGACAG 
TACGAGTTTG GGCTCACAAT TACGTGCCCC AACAAGGACA AAATTCACTT TAACGCTCGC 
AATTTCGAGG ATCGGTGTCA TTTTGTGGCC GATGTCACCG AATCGATTCG AGAAGCCACT 
GAAATGGAGC AAGTTCGGCT GGAAATGGAA ATGGAGCAGC ATACGACTAC GACGATTCGG 
CCGGATAATC AAAGAGATAG TGGACTTCCT GATATGGATG AATCTATGAA GCTTTCGAAC 
GGAAGCAACA GCAGCGCAGG CTCCTCAAAC TCTTCAACAA ACGGCGTAAA TAACTCTTCA 
TTCCGCCGAC TTTCGTTCAA TTCTCTGGAT AGTGGAGTCG TAGAAGAGCA TCTGGACGCT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_001307359.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294288.1 Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

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CloneID	OCa344453
Clone ID Related Accession (Same CDS sequence)	NM_001307359.1 , NM_001307359.2
Accession Version	NM_001307359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307359.1.

ATGAATTTAT TTTACGCAAT TCTCGCTATA ATCTCAAAGA TTTTTAACGC ATTAATCCGA 
TACGCCACAA GTCTCAGCGC ACAGCTCCGG ATAGACCGTG CCGCTCGTTC AACAACACTG 
GACAGAAGTC CGCCGTCACC GGCGCCGCCC GTCTTGGTCA ATTTGCAGAA TTCGAATATG 
ATTGACAGTC TAATGTCACC ACGGCTGGGA AATCGTCGAT TCGGAGCTGC CGCATCACGG 
TGTGCTCCGG CAGCCGCCGA GCTCACGAGC ACAGAAGTCT GGCTTCCGCG CCCGTCGCTT 
GTCAACCACA ATCCGGCACA CTCGAACTCG TTACCGCGGC TCGACAAGCA CAGAGTTCAT 
GAACGCAGCG AGTCATCAAA CGGCAGGAAA AAATCGGAGG TAAAGCCGCC ACGACAGATG 
ACAGATCAGG AGAGGAAACG GCAGTACAGG ATAGCCTTGA ACTTTTTCAA TAAAAAACCC 
GAGCGCGGCG TCCAACTGCT CACCGCATGG GGATTTGTGA AAAATTCGCC GGATTCCCTT 
GCCAGCCTAC TATTCGGACG TCGTGGACTG AGCAAATCAA TGATTGGAGA ATATATTGGA 
ACCCTACACT CACCCTATCA TAGTCTAGTT TTAAAATATT TCACCGCACT AATCGATATC 
CGAGGCCTAG AAGTGGATGT TGCCCTACGA AAAGCCATGC AATTCTTCAT TCTACCCAAA 
GAAGCCGAAA AAATCGATCG GATCATTCAA GCGTTCGCCC TACACTACTC AAAATCGAAT 
CCGAAGCGAA CATCACATTA TCGTGGTGGC TGGGATACAA TTCATCTACT CTCTTTTGCG 
ATTATCATGT TAAATACTGA CCTGCACTCA CCGAACGTGA AGCAACGAAT GACTCAGGCG 
GATTTTGTGA AAAATCTGAG AGGACAGGAT AAAATATCAG GGGAGAAAAA TGGAGAGGAT 
ATTGATCGAA AAACGCTTGA AGGGATCTAT GATCGGATAA AGAAGGACGA GTTGAAAGCT 
GGGGATGATC ATGTTGCACA AGTTCAAAGA GTTGATCGGG CTATTGTGGG AAAAGATAAA 
CCGCGCCTCA CCGAAACGCC CCGTCGGCTC GTCTGTTACT GCCGCCTTCA ACAAGTCTCC 
GATCCAACAA AACGTCAATC TTCATCAGCT CGCGAACGTG ACGTCTTCCT ATTCAATGAT 
ATGATCGTCG TGGCGAAAGG TGTTCGTCGT GGATCCACGT CAATGGCCGG TTGTACGTAC 
ACTCTGAAGC AGTGGACGCT TCTTCTCGGA GCACATGTCA CAGAATTCCA GCGAGGACAG 
TACGAGTTTG GGCTCACAAT TACGTGCCCC AACAAGGACA AAATTCACTT TAACGCTCGC 
AATTTCGAGG ATCGGTGTCA TTTTGTGGCC GATGTCACCG AATCGATTCG AGAAGCCACT 
GAAATGGAGC AAGTTCGGCT GGAAATGGAA ATGGAGCAGC ATACGACTAC GACGATTCGG 
CCGGATAATC AAAGAGATAG TGGACTTCCT GATATGGATG AATCTATGAA GCTTTCGAAC 
GGAAGCAACA GCAGCGCAGG CTCCTCAAAC TCTTCAACAA ACGGCGTAAA TAACTCTTCA 
TTCCGCCGAC TTTCGTTCAA TTCTCTGGAT AGTGGAGTCG TAGAAGAGCA TCTGGACGCT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294288.1
CDS	88..1773
Translation	MNLFYAILAIISKIFNALIRYATSLSAQLRIDRAARSTTLDRSPPSPAPPVLVNLQNSNMIDSLMSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_001307359.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307359.2

ORF Insert Sequence:

ATGAATTTAT TTTACGCAAT TCTCGCTATA ATCTCAAAGA TTTTTAACGC ATTAATCCGA 
TACGCCACAA GTCTCAGCGC ACAGCTCCGG ATAGACCGTG CCGCTCGTTC AACAACACTG 
GACAGAAGTC CGCCGTCACC GGCGCCGCCC GTCTTGGTCA ATTTGCAGAA TTCGAATATG 
ATTGACAGTC TAATGTCACC ACGGCTGGGA AATCGTCGAT TCGGAGCTGC CGCATCACGG 
TGTGCTCCGG CAGCCGCCGA GCTCACGAGC ACAGAAGTCT GGCTTCCGCG CCCGTCGCTT 
GTCAACCACA ATCCGGCACA CTCGAACTCG TTACCGCGGC TCGACAAGCA CAGAGTTCAT 
GAACGCAGCG AGTCATCAAA CGGCAGGAAA AAATCGGAGG TAAAGCCGCC ACGACAGATG 
ACAGATCAGG AGAGGAAACG GCAGTACAGG ATAGCCTTGA ACTTTTTCAA TAAAAAACCC 
GAGCGCGGCG TCCAACTGCT CACCGCATGG GGATTTGTGA AAAATTCGCC GGATTCCCTT 
GCCAGCCTAC TATTCGGACG TCGTGGACTG AGCAAATCAA TGATTGGAGA ATATATTGGA 
ACCCTACACT CACCCTATCA TAGTCTAGTT TTAAAATATT TCACCGCACT AATCGATATC 
CGAGGCCTAG AAGTGGATGT TGCCCTACGA AAAGCCATGC AATTCTTCAT TCTACCCAAA 
GAAGCCGAAA AAATCGATCG GATCATTCAA GCGTTCGCCC TACACTACTC AAAATCGAAT 
CCGAAGCGAA CATCACATTA TCGTGGTGGC TGGGATACAA TTCATCTACT CTCTTTTGCG 
ATTATCATGT TAAATACTGA CCTGCACTCA CCGAACGTGA AGCAACGAAT GACTCAGGCG 
GATTTTGTGA AAAATCTGAG AGGACAGGAT AAAATATCAG GGGAGAAAAA TGGAGAGGAT 
ATTGATCGAA AAACGCTTGA AGGGATCTAT GATCGGATAA AGAAGGACGA GTTGAAAGCT 
GGGGATGATC ATGTTGCACA AGTTCAAAGA GTTGATCGGG CTATTGTGGG AAAAGATAAA 
CCGCGCCTCA CCGAAACGCC CCGTCGGCTC GTCTGTTACT GCCGCCTTCA ACAAGTCTCC 
GATCCAACAA AACGTCAATC TTCATCAGCT CGCGAACGTG ACGTCTTCCT ATTCAATGAT 
ATGATCGTCG TGGCGAAAGG TGTTCGTCGT GGATCCACGT CAATGGCCGG TTGTACGTAC 
ACTCTGAAGC AGTGGACGCT TCTTCTCGGA GCACATGTCA CAGAATTCCA GCGAGGACAG 
TACGAGTTTG GGCTCACAAT TACGTGCCCC AACAAGGACA AAATTCACTT TAACGCTCGC 
AATTTCGAGG ATCGGTGTCA TTTTGTGGCC GATGTCACCG AATCGATTCG AGAAGCCACT 
GAAATGGAGC AAGTTCGGCT GGAAATGGAA ATGGAGCAGC ATACGACTAC GACGATTCGG 
CCGGATAATC AAAGAGATAG TGGACTTCCT GATATGGATG AATCTATGAA GCTTTCGAAC 
GGAAGCAACA GCAGCGCAGG CTCCTCAAAC TCTTCAACAA ACGGCGTAAA TAACTCTTCA 
TTCCGCCGAC TTTCGTTCAA TTCTCTGGAT AGTGGAGTCG TAGAAGAGCA TCTGGACGCT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_001307360.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294289.1 Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

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CloneID	OCa344454
Clone ID Related Accession (Same CDS sequence)	NM_001307360.1 , NM_001372781.1
Accession Version	NM_001307360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	BRag/Iqsec/Schizo related Arf GEF family member
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGATTGACA GTCTAATGTC ACCACGGCTG GGAAATCGTC GATTCGGAGC TGCCGCATCA 
CGGTGTGCTC CGGCAGCCGC CGAGCTCACG AGCACAGAAG TCTGGCTTCC GCGCCCGTCG 
CTTGTCAACC ACAATCCGGC ACACTCGAAC TCGTTACCGC GGCTCGACAA GCACAGAGTT 
CATGAACGCA GCGAGTCATC AAACGGCAGG AAAAAATCGG AGGTAAAGCC GCCACGACAG 
ATGACAGATC AGGAGAGGAA ACGGCAGTAC AGGATAGCCT TGAACTTTTT CAATAAAAAA 
CCCGAGCGCG GCGTCCAACT GCTCACCGCA TGGGGATTTG TGAAAAATTC GCCGGATTCC 
CTTGCCAGCC TACTATTCGG ACGTCGTGGA CTGAGCAAAT CAATGATTGG AGAATATATT 
GGAACCCTAC ACTCACCCTA TCATAGTCTA GTTTTAAAAT ATTTCACCGC ACTAATCGAT 
ATCCGAGGCC TAGAAGTGGA TGTTGCCCTA CGAAAAGCCA TGCAATTCTT CATTCTACCC 
AAAGAAGCCG AAAAAATCGA TCGGATCATT CAAGCGTTCG CCCTACACTA CTCAAAATCG 
AATCCGAAGC GAACATCACA TTATCGTGGT GGCTGGGATA CAATTCATCT ACTCTCTTTT 
GCGATTATCA TGTTAAATAC TGACCTGCAC TCACCGAACG TGAAGCAACG AATGACTCAG 
GCGGATTTTG TGAAAAATCT GAGAGGACAG GATAAAATAT CAGGGGAGAA AAATGGAGAG 
GATATTGATC GAAAAACGCT TGAAGGGATC TATGATCGGA TAAAGAAGGA CGAGTTGAAA 
GCTGGGGATG ATCATGTTGC ACAAGTTCAA AGAGTTGATC GGGCTATTGT GGGAAAAGAT 
AAACCGCGCC TCACCGAAAC GCCCCGTCGG CTCGTCTGTT ACTGCCGCCT TCAACAAGTC 
TCCGATCCAA CAAAACGTCA ATCTTCATCA GCTCGCGAAC GTGACGTCTT CCTATTCAAT 
GATATGATCG TCGTGGCGAA AGGTGTTCGT CGTGGATCCA CGTCAATGGC CGGTTGTACG 
TACACTCTGA AGCAGTGGAC GCTTCTTCTC GGAGCACATG TCACAGAATT CCAGCGAGGA 
CAGTACGAGT TTGGGCTCAC AATTACGTGC CCCAACAAGG ACAAAATTCA CTTTAACGCT 
CGCAATTTCG AGGATCGGTG TCATTTTGTG GCCGATGTCA CCGAATCGAT TCGAGAAGCC 
ACTGAAATGG AGCAAGTTCG GCTGGAAATG GAAATGGAGC AGCATACGAC TACGACGATT 
CGGCCGGATA ATCAAAGAGA TAGTGGACTT CCTGATATGG ATGAATCTAT GAAGCTTTCG 
AACGGAAGCA ACAGCAGCGC AGGCTCCTCA AACTCTTCAA CAAACGGCGT AAATAACTCT 
TCATTCCGCC GACTTTCGTT CAATTCTCTG GATAGTGGAG TCGTAGAAGA GCATCTGGAC 
GCTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294289.1
CDS	1..1509
Translation	MIDSLMSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_001307360.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans BRag/Iqsec/Schizo related Arf GEF family member (bris-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307360.1

ORF Insert Sequence:

ATGATTGACA GTCTAATGTC ACCACGGCTG GGAAATCGTC GATTCGGAGC TGCCGCATCA 
CGGTGTGCTC CGGCAGCCGC CGAGCTCACG AGCACAGAAG TCTGGCTTCC GCGCCCGTCG 
CTTGTCAACC ACAATCCGGC ACACTCGAAC TCGTTACCGC GGCTCGACAA GCACAGAGTT 
CATGAACGCA GCGAGTCATC AAACGGCAGG AAAAAATCGG AGGTAAAGCC GCCACGACAG 
ATGACAGATC AGGAGAGGAA ACGGCAGTAC AGGATAGCCT TGAACTTTTT CAATAAAAAA 
CCCGAGCGCG GCGTCCAACT GCTCACCGCA TGGGGATTTG TGAAAAATTC GCCGGATTCC 
CTTGCCAGCC TACTATTCGG ACGTCGTGGA CTGAGCAAAT CAATGATTGG AGAATATATT 
GGAACCCTAC ACTCACCCTA TCATAGTCTA GTTTTAAAAT ATTTCACCGC ACTAATCGAT 
ATCCGAGGCC TAGAAGTGGA TGTTGCCCTA CGAAAAGCCA TGCAATTCTT CATTCTACCC 
AAAGAAGCCG AAAAAATCGA TCGGATCATT CAAGCGTTCG CCCTACACTA CTCAAAATCG 
AATCCGAAGC GAACATCACA TTATCGTGGT GGCTGGGATA CAATTCATCT ACTCTCTTTT 
GCGATTATCA TGTTAAATAC TGACCTGCAC TCACCGAACG TGAAGCAACG AATGACTCAG 
GCGGATTTTG TGAAAAATCT GAGAGGACAG GATAAAATAT CAGGGGAGAA AAATGGAGAG 
GATATTGATC GAAAAACGCT TGAAGGGATC TATGATCGGA TAAAGAAGGA CGAGTTGAAA 
GCTGGGGATG ATCATGTTGC ACAAGTTCAA AGAGTTGATC GGGCTATTGT GGGAAAAGAT 
AAACCGCGCC TCACCGAAAC GCCCCGTCGG CTCGTCTGTT ACTGCCGCCT TCAACAAGTC 
TCCGATCCAA CAAAACGTCA ATCTTCATCA GCTCGCGAAC GTGACGTCTT CCTATTCAAT 
GATATGATCG TCGTGGCGAA AGGTGTTCGT CGTGGATCCA CGTCAATGGC CGGTTGTACG 
TACACTCTGA AGCAGTGGAC GCTTCTTCTC GGAGCACATG TCACAGAATT CCAGCGAGGA 
CAGTACGAGT TTGGGCTCAC AATTACGTGC CCCAACAAGG ACAAAATTCA CTTTAACGCT 
CGCAATTTCG AGGATCGGTG TCATTTTGTG GCCGATGTCA CCGAATCGAT TCGAGAAGCC 
ACTGAAATGG AGCAAGTTCG GCTGGAAATG GAAATGGAGC AGCATACGAC TACGACGATT 
CGGCCGGATA ATCAAAGAGA TAGTGGACTT CCTGATATGG ATGAATCTAT GAAGCTTTCG 
AACGGAAGCA ACAGCAGCGC AGGCTCCTCA AACTCTTCAA CAAACGGCGT AAATAACTCT 
TCATTCCGCC GACTTTCGTT CAATTCTCTG GATAGTGGAG TCGTAGAAGA GCATCTGGAC 
GCTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bris-1 ( NM_001372781.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360200.1 Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

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CloneID	OCa344454
Clone ID Related Accession (Same CDS sequence)	NM_001307360.1 , NM_001372781.1
Accession Version	NM_001372781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	SEC7 domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282).

ATGATTGACA GTCTAATGTC ACCACGGCTG GGAAATCGTC GATTCGGAGC TGCCGCATCA 
CGGTGTGCTC CGGCAGCCGC CGAGCTCACG AGCACAGAAG TCTGGCTTCC GCGCCCGTCG 
CTTGTCAACC ACAATCCGGC ACACTCGAAC TCGTTACCGC GGCTCGACAA GCACAGAGTT 
CATGAACGCA GCGAGTCATC AAACGGCAGG AAAAAATCGG AGGTAAAGCC GCCACGACAG 
ATGACAGATC AGGAGAGGAA ACGGCAGTAC AGGATAGCCT TGAACTTTTT CAATAAAAAA 
CCCGAGCGCG GCGTCCAACT GCTCACCGCA TGGGGATTTG TGAAAAATTC GCCGGATTCC 
CTTGCCAGCC TACTATTCGG ACGTCGTGGA CTGAGCAAAT CAATGATTGG AGAATATATT 
GGAACCCTAC ACTCACCCTA TCATAGTCTA GTTTTAAAAT ATTTCACCGC ACTAATCGAT 
ATCCGAGGCC TAGAAGTGGA TGTTGCCCTA CGAAAAGCCA TGCAATTCTT CATTCTACCC 
AAAGAAGCCG AAAAAATCGA TCGGATCATT CAAGCGTTCG CCCTACACTA CTCAAAATCG 
AATCCGAAGC GAACATCACA TTATCGTGGT GGCTGGGATA CAATTCATCT ACTCTCTTTT 
GCGATTATCA TGTTAAATAC TGACCTGCAC TCACCGAACG TGAAGCAACG AATGACTCAG 
GCGGATTTTG TGAAAAATCT GAGAGGACAG GATAAAATAT CAGGGGAGAA AAATGGAGAG 
GATATTGATC GAAAAACGCT TGAAGGGATC TATGATCGGA TAAAGAAGGA CGAGTTGAAA 
GCTGGGGATG ATCATGTTGC ACAAGTTCAA AGAGTTGATC GGGCTATTGT GGGAAAAGAT 
AAACCGCGCC TCACCGAAAC GCCCCGTCGG CTCGTCTGTT ACTGCCGCCT TCAACAAGTC 
TCCGATCCAA CAAAACGTCA ATCTTCATCA GCTCGCGAAC GTGACGTCTT CCTATTCAAT 
GATATGATCG TCGTGGCGAA AGGTGTTCGT CGTGGATCCA CGTCAATGGC CGGTTGTACG 
TACACTCTGA AGCAGTGGAC GCTTCTTCTC GGAGCACATG TCACAGAATT CCAGCGAGGA 
CAGTACGAGT TTGGGCTCAC AATTACGTGC CCCAACAAGG ACAAAATTCA CTTTAACGCT 
CGCAATTTCG AGGATCGGTG TCATTTTGTG GCCGATGTCA CCGAATCGAT TCGAGAAGCC 
ACTGAAATGG AGCAAGTTCG GCTGGAAATG GAAATGGAGC AGCATACGAC TACGACGATT 
CGGCCGGATA ATCAAAGAGA TAGTGGACTT CCTGATATGG ATGAATCTAT GAAGCTTTCG 
AACGGAAGCA ACAGCAGCGC AGGCTCCTCA AACTCTTCAA CAAACGGCGT AAATAACTCT 
TCATTCCGCC GACTTTCGTT CAATTCTCTG GATAGTGGAG TCGTAGAAGA GCATCTGGAC 
GCTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360200.1
CDS	353..1861
Translation	MIDSLMSPRLGNRRFGAAASRCAPAAAELTSTEVWLPRPSLVNHNPAHSNSLPRLDKHRVHERSESSNGRKKSEVKPPRQMTDQERKRQYRIALNFFNKKPERGVQLLTAWGFVKNSPDSLASLLFGRRGLSKSMIGEYIGTLHSPYHSLVLKYFTALIDIRGLEVDVALRKAMQFFILPKEAEKIDRIIQAFALHYSKSNPKRTSHYRGGWDTIHLLSFAIIMLNTDLHSPNVKQRMTQADFVKNLRGQDKISGEKNGEDIDRKTLEGIYDRIKKDELKAGDDHVAQVQRVDRAIVGKDKPRLTETPRRLVCYCRLQQVSDPTKRQSSSARERDVFLFNDMIVVAKGVRRGSTSMAGCTYTLKQWTLLLGAHVTEFQRGQYEFGLTITCPNKDKIHFNARNFEDRCHFVADVTESIREATEMEQVRLEMEMEQHTTTTIRPDNQRDSGLPDMDESMKLSNGSNSSAGSSNSSTNGVNNSSFRRLSFNSLDSGVVEEHLDAC

Target ORF information:

RefSeq Version	NM_001372781.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans SEC7 domain-containing protein (bris-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001372781.1

ORF Insert Sequence:

ATGATTGACA GTCTAATGTC ACCACGGCTG GGAAATCGTC GATTCGGAGC TGCCGCATCA 
CGGTGTGCTC CGGCAGCCGC CGAGCTCACG AGCACAGAAG TCTGGCTTCC GCGCCCGTCG 
CTTGTCAACC ACAATCCGGC ACACTCGAAC TCGTTACCGC GGCTCGACAA GCACAGAGTT 
CATGAACGCA GCGAGTCATC AAACGGCAGG AAAAAATCGG AGGTAAAGCC GCCACGACAG 
ATGACAGATC AGGAGAGGAA ACGGCAGTAC AGGATAGCCT TGAACTTTTT CAATAAAAAA 
CCCGAGCGCG GCGTCCAACT GCTCACCGCA TGGGGATTTG TGAAAAATTC GCCGGATTCC 
CTTGCCAGCC TACTATTCGG ACGTCGTGGA CTGAGCAAAT CAATGATTGG AGAATATATT 
GGAACCCTAC ACTCACCCTA TCATAGTCTA GTTTTAAAAT ATTTCACCGC ACTAATCGAT 
ATCCGAGGCC TAGAAGTGGA TGTTGCCCTA CGAAAAGCCA TGCAATTCTT CATTCTACCC 
AAAGAAGCCG AAAAAATCGA TCGGATCATT CAAGCGTTCG CCCTACACTA CTCAAAATCG 
AATCCGAAGC GAACATCACA TTATCGTGGT GGCTGGGATA CAATTCATCT ACTCTCTTTT 
GCGATTATCA TGTTAAATAC TGACCTGCAC TCACCGAACG TGAAGCAACG AATGACTCAG 
GCGGATTTTG TGAAAAATCT GAGAGGACAG GATAAAATAT CAGGGGAGAA AAATGGAGAG 
GATATTGATC GAAAAACGCT TGAAGGGATC TATGATCGGA TAAAGAAGGA CGAGTTGAAA 
GCTGGGGATG ATCATGTTGC ACAAGTTCAA AGAGTTGATC GGGCTATTGT GGGAAAAGAT 
AAACCGCGCC TCACCGAAAC GCCCCGTCGG CTCGTCTGTT ACTGCCGCCT TCAACAAGTC 
TCCGATCCAA CAAAACGTCA ATCTTCATCA GCTCGCGAAC GTGACGTCTT CCTATTCAAT 
GATATGATCG TCGTGGCGAA AGGTGTTCGT CGTGGATCCA CGTCAATGGC CGGTTGTACG 
TACACTCTGA AGCAGTGGAC GCTTCTTCTC GGAGCACATG TCACAGAATT CCAGCGAGGA 
CAGTACGAGT TTGGGCTCAC AATTACGTGC CCCAACAAGG ACAAAATTCA CTTTAACGCT 
CGCAATTTCG AGGATCGGTG TCATTTTGTG GCCGATGTCA CCGAATCGAT TCGAGAAGCC 
ACTGAAATGG AGCAAGTTCG GCTGGAAATG GAAATGGAGC AGCATACGAC TACGACGATT 
CGGCCGGATA ATCAAAGAGA TAGTGGACTT CCTGATATGG ATGAATCTAT GAAGCTTTCG 
AACGGAAGCA ACAGCAGCGC AGGCTCCTCA AACTCTTCAA CAAACGGCGT AAATAACTCT 
TCATTCCGCC GACTTTCGTT CAATTCTCTG GATAGTGGAG TCGTAGAAGA GCATCTGGAC 
GCTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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bris-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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