smc-5 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	smc-5
Entrez Gene ID	173874
Full Name	Structural maintenance of chromosomes protein 5
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_062534.4	NP_494935.1	Structural maintenance of chromosomes protein 5
NM_062534.5	NP_494935.1	Structural maintenance of chromosomes protein 5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest that SMC-5/6 and HIM-6 can synergistically regulate recombination intermediate metabolism and suppress ectopic recombination by controlling chromosome architecture during meiosis.
Title: The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis.

Data propose that SMC-5/6 promotes replication fork stability and facilitates recombination-dependent repair.
Title: Loss of Caenorhabditis elegans BRCA1 promotes genome stability during replication in smc-5 mutants.

The following smc-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smc-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338507	NM_062534.5 Latest version!	Caenorhabditis elegans Structural maintenance of chromosomes protein 5 (smc-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa338507	NM_062534.4	Caenorhabditis elegans Structural maintenance of chromosomes protein 5 (smc-5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smc-5 ( NM_062534.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494935.1 Caenorhabditis elegans Structural maintenance of chromosomes protein 5 (smc-5), partial mRNA.

Price & Availability↑

CloneID	OCa338507
Clone ID Related Accession (Same CDS sequence)	NM_062534.4 , NM_062534.5
Accession Version	NM_062534.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Structural maintenance of chromosomes protein 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_062534.3. COMPLETENESS: incomplete on both ends.

ATGGCCTCGC AAGACAGCGA TGAGGCTTTG CCAGCAAATT ATAAAGATTA TCCGGATGGA 
TCTCTCCTTC GCGTTGTATT TCATAATTTT CTAACATACG AACACACCTC ATTCCTTCCA 
ACTGCAAGTC TGAATATGAT TTTGGGACAT AATGGAAGTG GAAAAAGTTC GATTATTTGT 
GGAATTTGTC TGGCTTGCGG CGGAAGTCCG AAATCATTGG GAAGATCAGA GAGAATCGTG 
GAATATATTA GACATGGATG CACAGAAGGA TACGTTGAAA TTGCAATTGC TGACAAACAA 
AAAGGTCCAC AAGTTGTTCG CTTAACGATT CGAGTGGGAG AGCAGCCGAA ATATCGTTTA 
AATGATAGTG CAACAACACA AAGTGAAATC GCCGATCTAA GAAAGCATTA CAATATTCAA 
ATTGACAATC CGTGTGCATT TCTTGCTCAA GACAAAGTCA AGAGTTTTTC CGAGCAATCG 
TCTATTGAAT TGTTAAGAAA TACAGAAAAA GCTGCGTCGG CTGATCTCGA TCAACAACAC 
ATAGATCTCA TGAAACAGCG AGAAGACTCG ACTTCAATTG AGGATAAATG TACAACATCT 
GAAAATGCAA TTAAACGATT AGAAGATGAA ATTGGTAAAA TTATGCCACT CGTTGAGAAC 
TATCGAAAGA AGTTAGCTCT CCAGTCAAAA CTCCGTTTGC TCGAAAAGAA AATGAAAATT 
ATGGAATTTG AAAAGTTCGA TCGGGAGTAT AAAGCTGAGT TGCAAAACAT GGATGGAGCA 
ATGATAGAGT ATCGAGAAGT CGAGAAAAGT ATTGCAGAAT GCGAAAAGCA CAGAAAAAAT 
TTGGAAGATC GGATTAAAAA AGACAGAAGT CAAATCAGTC AGATGCAACG TTCTTGTAAC 
GAAATCCTTG CAAAAGTCCA AGAGAAAGGA GATAAAAAGT TGATGGAAGA TATGATGCAA 
CGTGCTAAAG CAAAATTGGA ATCGGCGAAA AAAGCAGCAG ATCAACATGA AAAAGATGTC 
GAAAAAGCTC GAAAAATGAT TGATCAAGCT CGAGCGAGAC TTCAGGAAGC CGTTGATACT 
CTTAATGGAT ACGAAGAGTT TCAATCCGAA ATGAAGAGTT TAGAGCAAAA GTATTCGACT 
GCTGAACGTG ACAGTCGTCA AGAAGAAGAT GCAATTCAAA AGAAATCATA TGAAATGCGG 
CAGCTGGAAA ATAAAAAACG AGACGAAGAA CAGAATTCTC AGTTAAATCG ACAAGATCGA 
TATCGCGTTC TTCAAAATTT CAGCAGTGAT GCCTCGAAAG CCTATCGCTG GTATCAACAG 
AATCGAAGTC AATTCAAAGG AGACGTATAT ATGCCCATTA TGGATATGGT TCTCAAAACT 
CCAGAAGCGG CAAAAGCTCT TGAGAACAGT GTTGGTGTTC GTGATCGTAC TATGTTCGTT 
TGCTGTTATA AGGAAGATGA ACTTTTGATC AACGGAAAAC AACATTCATG GCGTATTAAC 
ACTTCAGTGG TCCCTGCTGA AAAAATCTAT TCTGAAGATA TCGATGCTCA GCTTCCATCT 
GAACTTTCTC GTCTTGGATT TAAATATCTC GTTTCTAATT GCTTTGATGC TCCTGCCCCA 
CTCAAACAAT TCTTATGCAA TGTCAGCGGT TTGAATAGAA TTCCATTCGG AGGATCTGAT 
GTTGAGAAAA AGATCGCTGA AGTATCGCAA GCGATCGAAC AAACGAGATA TTCAGTTTTT 
TTGACAGCCA ATATTAGATG TCAAAACTCG AAATCCAGAT ATGCAAATAA CACTCTTCAA 
TCTCAATCGG CCACACGCGA AGCAAATACA TGGCGAGACC AGTTCTTCAA AGTTTCTCCA 
GTTGCGAAGA GAACCGACAA CAGTATTCTT GAAGAAATTC AAAAACTGAA AGCAGAAATC 
GATATTCGAT CTGAACAACT ACGAGAGAAA CGTGGAGCAA TTCAGAAGGA ACGAGATGTT 
TTACGTCAAG AGCAAATGCA ATGGAAGTCG AAGAAACAAG TTCATACTAA ATGGAAAACA 
GAATTGGCGA GTGAAATGGC AAAATTGGAA GCTCTCGAAA ATGAAGTTGT TGATATTTCT 
GCAATTGAAG AAGAGTATGC GAATGTGGAA AAGAAGGCGA TTCTTGAGAC GAAAAAGATG 
CTGGAGAACA GTATTCGGTG GCATAAAGAA ATCATCGACA AGCATCGTTT AATTGGAATT 
TTCGAGCTGA GTGAATCCAT ATGCAAATCG AGAGTGAATA AATCAAATTC GGAAGCTGAA 
ACCCATCGAT CGAAGCTTGA AGATTTGAAA AGCGTCAAAG ATGCTGCTGA AGATCTACTG 
AAGACTGCGT TGAATCACAA GAAAGCAGCA GCAAGTGCAT TGATGAAGGA ATGTTCTTTG 
AAAACTTTAG ACGAATCGAA AATGAGTCCC GCTGAAAATA AGATTTATTC ATCTTTAGTT 
AAAATGTTCG AGGAAGCTGA TGTTCCGACT GATATGAATA CTCTGGATCA AGCAATCACT 
TCAGAGAAAA CCCGTTTGAA ACTTGCTGAA GATAGCGGCG AAGATGGTTC AATCGTGCAT 
GAACAGAGAT TAAAAGTTCT TGATGATGAT CTCGTTTTGG AAAAAACGCG TCAAGAGAAG 
CTTATTGAAA ACCGTGCACG TATCCACGAT AAACTAGGTG ATGAGATCAA CAATTGGCGT 
AAGGAAGTTG AGACAATGAT TGAACAAATC AATGTAAACT ACGTTCAATT CTTTGATTCT 
CTTGGTTGTC GCGGAGAAGT TAGTCTTGAA GTTCCCGAAA ATCCACTGGA TATTGAAAAA 
TATGGAATCA TGATTATGGT GTGCTTCCGC AAAGGAGAAA GTATGAAACG TCTTGACAAT 
AAAGTTCAAT CTGGAGGTGA ACGAAGTGTG GCTACGATGC TTTATCTTCT GGCTCTACAA 
CAATTATGCC CAGTTCCATT CAGATGTATT GATGAGATTA ATCAGGGAAT GGATCCTACA 
AACGAACGGA AAGTGTTTGA TATTATGGTT GGAATGTGGA ATGGAACCAC TGGAACACTT 
TCCAAAACTC AATACTTTTT GCTCTCTCCA AAACTTCTTC ATGGTTTGGA TATGCGCGAA 
AATGTGAACA TTGTTATGGT GAATAGTACA CTCACCAATT CTCATGGAAA GCATTACGAC 
ACCAGTGCCA AGATAGATGC GACATTTGCA AAAATGGGAA TCAGTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494935.1
CDS	1..3231
Translation	MASQDSDEALPANYKDYPDGSLLRVVFHNFLTYEHTSFLPTASLNMILGHNGSGKSSIICGICLACGGSPKSLGRSERIVEYIRHGCTEGYVEIAIADKQKGPQVVRLTIRVGEQPKYRLNDSATTQSEIADLRKHYNIQIDNPCAFLAQDKVKSFSEQSSIELLRNTEKAASADLDQQHIDLMKQREDSTSIEDKCTTSENAIKRLEDEIGKIMPLVENYRKKLALQSKLRLLEKKMKIMEFEKFDREYKAELQNMDGAMIEYREVEKSIAECEKHRKNLEDRIKKDRSQISQMQRSCNEILAKVQEKGDKKLMEDMMQRAKAKLESAKKAADQHEKDVEKARKMIDQARARLQEAVDTLNGYEEFQSEMKSLEQKYSTAERDSRQEEDAIQKKSYEMRQLENKKRDEEQNSQLNRQDRYRVLQNFSSDASKAYRWYQQNRSQFKGDVYMPIMDMVLKTPEAAKALENSVGVRDRTMFVCCYKEDELLINGKQHSWRINTSVVPAEKIYSEDIDAQLPSELSRLGFKYLVSNCFDAPAPLKQFLCNVSGLNRIPFGGSDVEKKIAEVSQAIEQTRYSVFLTANIRCQNSKSRYANNTLQSQSATREANTWRDQFFKVSPVAKRTDNSILEEIQKLKAEIDIRSEQLREKRGAIQKERDVLRQEQMQWKSKKQVHTKWKTELASEMAKLEALENEVVDISAIEEEYANVEKKAILETKKMLENSIRWHKEIIDKHRLIGIFELSESICKSRVNKSNSEAETHRSKLEDLKSVKDAAEDLLKTALNHKKAAASALMKECSLKTLDESKMSPAENKIYSSLVKMFEEADVPTDMNTLDQAITSEKTRLKLAEDSGEDGSIVHEQRLKVLDDDLVLEKTRQEKLIENRARIHDKLGDEINNWRKEVETMIEQINVNYVQFFDSLGCRGEVSLEVPENPLDIEKYGIMIMVCFRKGESMKRLDNKVQSGGERSVATMLYLLALQQLCPVPFRCIDEINQGMDPTNERKVFDIMVGMWNGTTGTLSKTQYFLLSPKLLHGLDMRENVNIVMVNSTLTNSHGKHYDTSAKIDATFAKMGISA

Target ORF information:

RefSeq Version	NM_062534.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Structural maintenance of chromosomes protein 5 (smc-5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062534.4

ORF Insert Sequence:

ATGGCCTCGC AAGACAGCGA TGAGGCTTTG CCAGCAAATT ATAAAGATTA TCCGGATGGA 
TCTCTCCTTC GCGTTGTATT TCATAATTTT CTAACATACG AACACACCTC ATTCCTTCCA 
ACTGCAAGTC TGAATATGAT TTTGGGACAT AATGGAAGTG GAAAAAGTTC GATTATTTGT 
GGAATTTGTC TGGCTTGCGG CGGAAGTCCG AAATCATTGG GAAGATCAGA GAGAATCGTG 
GAATATATTA GACATGGATG CACAGAAGGA TACGTTGAAA TTGCAATTGC TGACAAACAA 
AAAGGTCCAC AAGTTGTTCG CTTAACGATT CGAGTGGGAG AGCAGCCGAA ATATCGTTTA 
AATGATAGTG CAACAACACA AAGTGAAATC GCCGATCTAA GAAAGCATTA CAATATTCAA 
ATTGACAATC CGTGTGCATT TCTTGCTCAA GACAAAGTCA AGAGTTTTTC CGAGCAATCG 
TCTATTGAAT TGTTAAGAAA TACAGAAAAA GCTGCGTCGG CTGATCTCGA TCAACAACAC 
ATAGATCTCA TGAAACAGCG AGAAGACTCG ACTTCAATTG AGGATAAATG TACAACATCT 
GAAAATGCAA TTAAACGATT AGAAGATGAA ATTGGTAAAA TTATGCCACT CGTTGAGAAC 
TATCGAAAGA AGTTAGCTCT CCAGTCAAAA CTCCGTTTGC TCGAAAAGAA AATGAAAATT 
ATGGAATTTG AAAAGTTCGA TCGGGAGTAT AAAGCTGAGT TGCAAAACAT GGATGGAGCA 
ATGATAGAGT ATCGAGAAGT CGAGAAAAGT ATTGCAGAAT GCGAAAAGCA CAGAAAAAAT 
TTGGAAGATC GGATTAAAAA AGACAGAAGT CAAATCAGTC AGATGCAACG TTCTTGTAAC 
GAAATCCTTG CAAAAGTCCA AGAGAAAGGA GATAAAAAGT TGATGGAAGA TATGATGCAA 
CGTGCTAAAG CAAAATTGGA ATCGGCGAAA AAAGCAGCAG ATCAACATGA AAAAGATGTC 
GAAAAAGCTC GAAAAATGAT TGATCAAGCT CGAGCGAGAC TTCAGGAAGC CGTTGATACT 
CTTAATGGAT ACGAAGAGTT TCAATCCGAA ATGAAGAGTT TAGAGCAAAA GTATTCGACT 
GCTGAACGTG ACAGTCGTCA AGAAGAAGAT GCAATTCAAA AGAAATCATA TGAAATGCGG 
CAGCTGGAAA ATAAAAAACG AGACGAAGAA CAGAATTCTC AGTTAAATCG ACAAGATCGA 
TATCGCGTTC TTCAAAATTT CAGCAGTGAT GCCTCGAAAG CCTATCGCTG GTATCAACAG 
AATCGAAGTC AATTCAAAGG AGACGTATAT ATGCCCATTA TGGATATGGT TCTCAAAACT 
CCAGAAGCGG CAAAAGCTCT TGAGAACAGT GTTGGTGTTC GTGATCGTAC TATGTTCGTT 
TGCTGTTATA AGGAAGATGA ACTTTTGATC AACGGAAAAC AACATTCATG GCGTATTAAC 
ACTTCAGTGG TCCCTGCTGA AAAAATCTAT TCTGAAGATA TCGATGCTCA GCTTCCATCT 
GAACTTTCTC GTCTTGGATT TAAATATCTC GTTTCTAATT GCTTTGATGC TCCTGCCCCA 
CTCAAACAAT TCTTATGCAA TGTCAGCGGT TTGAATAGAA TTCCATTCGG AGGATCTGAT 
GTTGAGAAAA AGATCGCTGA AGTATCGCAA GCGATCGAAC AAACGAGATA TTCAGTTTTT 
TTGACAGCCA ATATTAGATG TCAAAACTCG AAATCCAGAT ATGCAAATAA CACTCTTCAA 
TCTCAATCGG CCACACGCGA AGCAAATACA TGGCGAGACC AGTTCTTCAA AGTTTCTCCA 
GTTGCGAAGA GAACCGACAA CAGTATTCTT GAAGAAATTC AAAAACTGAA AGCAGAAATC 
GATATTCGAT CTGAACAACT ACGAGAGAAA CGTGGAGCAA TTCAGAAGGA ACGAGATGTT 
TTACGTCAAG AGCAAATGCA ATGGAAGTCG AAGAAACAAG TTCATACTAA ATGGAAAACA 
GAATTGGCGA GTGAAATGGC AAAATTGGAA GCTCTCGAAA ATGAAGTTGT TGATATTTCT 
GCAATTGAAG AAGAGTATGC GAATGTGGAA AAGAAGGCGA TTCTTGAGAC GAAAAAGATG 
CTGGAGAACA GTATTCGGTG GCATAAAGAA ATCATCGACA AGCATCGTTT AATTGGAATT 
TTCGAGCTGA GTGAATCCAT ATGCAAATCG AGAGTGAATA AATCAAATTC GGAAGCTGAA 
ACCCATCGAT CGAAGCTTGA AGATTTGAAA AGCGTCAAAG ATGCTGCTGA AGATCTACTG 
AAGACTGCGT TGAATCACAA GAAAGCAGCA GCAAGTGCAT TGATGAAGGA ATGTTCTTTG 
AAAACTTTAG ACGAATCGAA AATGAGTCCC GCTGAAAATA AGATTTATTC ATCTTTAGTT 
AAAATGTTCG AGGAAGCTGA TGTTCCGACT GATATGAATA CTCTGGATCA AGCAATCACT 
TCAGAGAAAA CCCGTTTGAA ACTTGCTGAA GATAGCGGCG AAGATGGTTC AATCGTGCAT 
GAACAGAGAT TAAAAGTTCT TGATGATGAT CTCGTTTTGG AAAAAACGCG TCAAGAGAAG 
CTTATTGAAA ACCGTGCACG TATCCACGAT AAACTAGGTG ATGAGATCAA CAATTGGCGT 
AAGGAAGTTG AGACAATGAT TGAACAAATC AATGTAAACT ACGTTCAATT CTTTGATTCT 
CTTGGTTGTC GCGGAGAAGT TAGTCTTGAA GTTCCCGAAA ATCCACTGGA TATTGAAAAA 
TATGGAATCA TGATTATGGT GTGCTTCCGC AAAGGAGAAA GTATGAAACG TCTTGACAAT 
AAAGTTCAAT CTGGAGGTGA ACGAAGTGTG GCTACGATGC TTTATCTTCT GGCTCTACAA 
CAATTATGCC CAGTTCCATT CAGATGTATT GATGAGATTA ATCAGGGAAT GGATCCTACA 
AACGAACGGA AAGTGTTTGA TATTATGGTT GGAATGTGGA ATGGAACCAC TGGAACACTT 
TCCAAAACTC AATACTTTTT GCTCTCTCCA AAACTTCTTC ATGGTTTGGA TATGCGCGAA 
AATGTGAACA TTGTTATGGT GAATAGTACA CTCACCAATT CTCATGGAAA GCATTACGAC 
ACCAGTGCCA AGATAGATGC GACATTTGCA AAAATGGGAA TCAGTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smc-5 ( NM_062534.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_494935.1 Caenorhabditis elegans Structural maintenance of chromosomes protein 5 (smc-5), mRNA.

Price & Availability↑

CloneID	OCa338507
Clone ID Related Accession (Same CDS sequence)	NM_062534.4 , NM_062534.5
Accession Version	NM_062534.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Structural maintenance of chromosomes protein 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_062534.4.

ATGGCCTCGC AAGACAGCGA TGAGGCTTTG CCAGCAAATT ATAAAGATTA TCCGGATGGA 
TCTCTCCTTC GCGTTGTATT TCATAATTTT CTAACATACG AACACACCTC ATTCCTTCCA 
ACTGCAAGTC TGAATATGAT TTTGGGACAT AATGGAAGTG GAAAAAGTTC GATTATTTGT 
GGAATTTGTC TGGCTTGCGG CGGAAGTCCG AAATCATTGG GAAGATCAGA GAGAATCGTG 
GAATATATTA GACATGGATG CACAGAAGGA TACGTTGAAA TTGCAATTGC TGACAAACAA 
AAAGGTCCAC AAGTTGTTCG CTTAACGATT CGAGTGGGAG AGCAGCCGAA ATATCGTTTA 
AATGATAGTG CAACAACACA AAGTGAAATC GCCGATCTAA GAAAGCATTA CAATATTCAA 
ATTGACAATC CGTGTGCATT TCTTGCTCAA GACAAAGTCA AGAGTTTTTC CGAGCAATCG 
TCTATTGAAT TGTTAAGAAA TACAGAAAAA GCTGCGTCGG CTGATCTCGA TCAACAACAC 
ATAGATCTCA TGAAACAGCG AGAAGACTCG ACTTCAATTG AGGATAAATG TACAACATCT 
GAAAATGCAA TTAAACGATT AGAAGATGAA ATTGGTAAAA TTATGCCACT CGTTGAGAAC 
TATCGAAAGA AGTTAGCTCT CCAGTCAAAA CTCCGTTTGC TCGAAAAGAA AATGAAAATT 
ATGGAATTTG AAAAGTTCGA TCGGGAGTAT AAAGCTGAGT TGCAAAACAT GGATGGAGCA 
ATGATAGAGT ATCGAGAAGT CGAGAAAAGT ATTGCAGAAT GCGAAAAGCA CAGAAAAAAT 
TTGGAAGATC GGATTAAAAA AGACAGAAGT CAAATCAGTC AGATGCAACG TTCTTGTAAC 
GAAATCCTTG CAAAAGTCCA AGAGAAAGGA GATAAAAAGT TGATGGAAGA TATGATGCAA 
CGTGCTAAAG CAAAATTGGA ATCGGCGAAA AAAGCAGCAG ATCAACATGA AAAAGATGTC 
GAAAAAGCTC GAAAAATGAT TGATCAAGCT CGAGCGAGAC TTCAGGAAGC CGTTGATACT 
CTTAATGGAT ACGAAGAGTT TCAATCCGAA ATGAAGAGTT TAGAGCAAAA GTATTCGACT 
GCTGAACGTG ACAGTCGTCA AGAAGAAGAT GCAATTCAAA AGAAATCATA TGAAATGCGG 
CAGCTGGAAA ATAAAAAACG AGACGAAGAA CAGAATTCTC AGTTAAATCG ACAAGATCGA 
TATCGCGTTC TTCAAAATTT CAGCAGTGAT GCCTCGAAAG CCTATCGCTG GTATCAACAG 
AATCGAAGTC AATTCAAAGG AGACGTATAT ATGCCCATTA TGGATATGGT TCTCAAAACT 
CCAGAAGCGG CAAAAGCTCT TGAGAACAGT GTTGGTGTTC GTGATCGTAC TATGTTCGTT 
TGCTGTTATA AGGAAGATGA ACTTTTGATC AACGGAAAAC AACATTCATG GCGTATTAAC 
ACTTCAGTGG TCCCTGCTGA AAAAATCTAT TCTGAAGATA TCGATGCTCA GCTTCCATCT 
GAACTTTCTC GTCTTGGATT TAAATATCTC GTTTCTAATT GCTTTGATGC TCCTGCCCCA 
CTCAAACAAT TCTTATGCAA TGTCAGCGGT TTGAATAGAA TTCCATTCGG AGGATCTGAT 
GTTGAGAAAA AGATCGCTGA AGTATCGCAA GCGATCGAAC AAACGAGATA TTCAGTTTTT 
TTGACAGCCA ATATTAGATG TCAAAACTCG AAATCCAGAT ATGCAAATAA CACTCTTCAA 
TCTCAATCGG CCACACGCGA AGCAAATACA TGGCGAGACC AGTTCTTCAA AGTTTCTCCA 
GTTGCGAAGA GAACCGACAA CAGTATTCTT GAAGAAATTC AAAAACTGAA AGCAGAAATC 
GATATTCGAT CTGAACAACT ACGAGAGAAA CGTGGAGCAA TTCAGAAGGA ACGAGATGTT 
TTACGTCAAG AGCAAATGCA ATGGAAGTCG AAGAAACAAG TTCATACTAA ATGGAAAACA 
GAATTGGCGA GTGAAATGGC AAAATTGGAA GCTCTCGAAA ATGAAGTTGT TGATATTTCT 
GCAATTGAAG AAGAGTATGC GAATGTGGAA AAGAAGGCGA TTCTTGAGAC GAAAAAGATG 
CTGGAGAACA GTATTCGGTG GCATAAAGAA ATCATCGACA AGCATCGTTT AATTGGAATT 
TTCGAGCTGA GTGAATCCAT ATGCAAATCG AGAGTGAATA AATCAAATTC GGAAGCTGAA 
ACCCATCGAT CGAAGCTTGA AGATTTGAAA AGCGTCAAAG ATGCTGCTGA AGATCTACTG 
AAGACTGCGT TGAATCACAA GAAAGCAGCA GCAAGTGCAT TGATGAAGGA ATGTTCTTTG 
AAAACTTTAG ACGAATCGAA AATGAGTCCC GCTGAAAATA AGATTTATTC ATCTTTAGTT 
AAAATGTTCG AGGAAGCTGA TGTTCCGACT GATATGAATA CTCTGGATCA AGCAATCACT 
TCAGAGAAAA CCCGTTTGAA ACTTGCTGAA GATAGCGGCG AAGATGGTTC AATCGTGCAT 
GAACAGAGAT TAAAAGTTCT TGATGATGAT CTCGTTTTGG AAAAAACGCG TCAAGAGAAG 
CTTATTGAAA ACCGTGCACG TATCCACGAT AAACTAGGTG ATGAGATCAA CAATTGGCGT 
AAGGAAGTTG AGACAATGAT TGAACAAATC AATGTAAACT ACGTTCAATT CTTTGATTCT 
CTTGGTTGTC GCGGAGAAGT TAGTCTTGAA GTTCCCGAAA ATCCACTGGA TATTGAAAAA 
TATGGAATCA TGATTATGGT GTGCTTCCGC AAAGGAGAAA GTATGAAACG TCTTGACAAT 
AAAGTTCAAT CTGGAGGTGA ACGAAGTGTG GCTACGATGC TTTATCTTCT GGCTCTACAA 
CAATTATGCC CAGTTCCATT CAGATGTATT GATGAGATTA ATCAGGGAAT GGATCCTACA 
AACGAACGGA AAGTGTTTGA TATTATGGTT GGAATGTGGA ATGGAACCAC TGGAACACTT 
TCCAAAACTC AATACTTTTT GCTCTCTCCA AAACTTCTTC ATGGTTTGGA TATGCGCGAA 
AATGTGAACA TTGTTATGGT GAATAGTACA CTCACCAATT CTCATGGAAA GCATTACGAC 
ACCAGTGCCA AGATAGATGC GACATTTGCA AAAATGGGAA TCAGTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_494935.1
CDS	13..3243
Translation	MASQDSDEALPANYKDYPDGSLLRVVFHNFLTYEHTSFLPTASLNMILGHNGSGKSSIICGICLACGGSPKSLGRSERIVEYIRHGCTEGYVEIAIADKQKGPQVVRLTIRVGEQPKYRLNDSATTQSEIADLRKHYNIQIDNPCAFLAQDKVKSFSEQSSIELLRNTEKAASADLDQQHIDLMKQREDSTSIEDKCTTSENAIKRLEDEIGKIMPLVENYRKKLALQSKLRLLEKKMKIMEFEKFDREYKAELQNMDGAMIEYREVEKSIAECEKHRKNLEDRIKKDRSQISQMQRSCNEILAKVQEKGDKKLMEDMMQRAKAKLESAKKAADQHEKDVEKARKMIDQARARLQEAVDTLNGYEEFQSEMKSLEQKYSTAERDSRQEEDAIQKKSYEMRQLENKKRDEEQNSQLNRQDRYRVLQNFSSDASKAYRWYQQNRSQFKGDVYMPIMDMVLKTPEAAKALENSVGVRDRTMFVCCYKEDELLINGKQHSWRINTSVVPAEKIYSEDIDAQLPSELSRLGFKYLVSNCFDAPAPLKQFLCNVSGLNRIPFGGSDVEKKIAEVSQAIEQTRYSVFLTANIRCQNSKSRYANNTLQSQSATREANTWRDQFFKVSPVAKRTDNSILEEIQKLKAEIDIRSEQLREKRGAIQKERDVLRQEQMQWKSKKQVHTKWKTELASEMAKLEALENEVVDISAIEEEYANVEKKAILETKKMLENSIRWHKEIIDKHRLIGIFELSESICKSRVNKSNSEAETHRSKLEDLKSVKDAAEDLLKTALNHKKAAASALMKECSLKTLDESKMSPAENKIYSSLVKMFEEADVPTDMNTLDQAITSEKTRLKLAEDSGEDGSIVHEQRLKVLDDDLVLEKTRQEKLIENRARIHDKLGDEINNWRKEVETMIEQINVNYVQFFDSLGCRGEVSLEVPENPLDIEKYGIMIMVCFRKGESMKRLDNKVQSGGERSVATMLYLLALQQLCPVPFRCIDEINQGMDPTNERKVFDIMVGMWNGTTGTLSKTQYFLLSPKLLHGLDMRENVNIVMVNSTLTNSHGKHYDTSAKIDATFAKMGISA

Target ORF information:

RefSeq Version	NM_062534.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Structural maintenance of chromosomes protein 5 (smc-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062534.5

ORF Insert Sequence:

ATGGCCTCGC AAGACAGCGA TGAGGCTTTG CCAGCAAATT ATAAAGATTA TCCGGATGGA 
TCTCTCCTTC GCGTTGTATT TCATAATTTT CTAACATACG AACACACCTC ATTCCTTCCA 
ACTGCAAGTC TGAATATGAT TTTGGGACAT AATGGAAGTG GAAAAAGTTC GATTATTTGT 
GGAATTTGTC TGGCTTGCGG CGGAAGTCCG AAATCATTGG GAAGATCAGA GAGAATCGTG 
GAATATATTA GACATGGATG CACAGAAGGA TACGTTGAAA TTGCAATTGC TGACAAACAA 
AAAGGTCCAC AAGTTGTTCG CTTAACGATT CGAGTGGGAG AGCAGCCGAA ATATCGTTTA 
AATGATAGTG CAACAACACA AAGTGAAATC GCCGATCTAA GAAAGCATTA CAATATTCAA 
ATTGACAATC CGTGTGCATT TCTTGCTCAA GACAAAGTCA AGAGTTTTTC CGAGCAATCG 
TCTATTGAAT TGTTAAGAAA TACAGAAAAA GCTGCGTCGG CTGATCTCGA TCAACAACAC 
ATAGATCTCA TGAAACAGCG AGAAGACTCG ACTTCAATTG AGGATAAATG TACAACATCT 
GAAAATGCAA TTAAACGATT AGAAGATGAA ATTGGTAAAA TTATGCCACT CGTTGAGAAC 
TATCGAAAGA AGTTAGCTCT CCAGTCAAAA CTCCGTTTGC TCGAAAAGAA AATGAAAATT 
ATGGAATTTG AAAAGTTCGA TCGGGAGTAT AAAGCTGAGT TGCAAAACAT GGATGGAGCA 
ATGATAGAGT ATCGAGAAGT CGAGAAAAGT ATTGCAGAAT GCGAAAAGCA CAGAAAAAAT 
TTGGAAGATC GGATTAAAAA AGACAGAAGT CAAATCAGTC AGATGCAACG TTCTTGTAAC 
GAAATCCTTG CAAAAGTCCA AGAGAAAGGA GATAAAAAGT TGATGGAAGA TATGATGCAA 
CGTGCTAAAG CAAAATTGGA ATCGGCGAAA AAAGCAGCAG ATCAACATGA AAAAGATGTC 
GAAAAAGCTC GAAAAATGAT TGATCAAGCT CGAGCGAGAC TTCAGGAAGC CGTTGATACT 
CTTAATGGAT ACGAAGAGTT TCAATCCGAA ATGAAGAGTT TAGAGCAAAA GTATTCGACT 
GCTGAACGTG ACAGTCGTCA AGAAGAAGAT GCAATTCAAA AGAAATCATA TGAAATGCGG 
CAGCTGGAAA ATAAAAAACG AGACGAAGAA CAGAATTCTC AGTTAAATCG ACAAGATCGA 
TATCGCGTTC TTCAAAATTT CAGCAGTGAT GCCTCGAAAG CCTATCGCTG GTATCAACAG 
AATCGAAGTC AATTCAAAGG AGACGTATAT ATGCCCATTA TGGATATGGT TCTCAAAACT 
CCAGAAGCGG CAAAAGCTCT TGAGAACAGT GTTGGTGTTC GTGATCGTAC TATGTTCGTT 
TGCTGTTATA AGGAAGATGA ACTTTTGATC AACGGAAAAC AACATTCATG GCGTATTAAC 
ACTTCAGTGG TCCCTGCTGA AAAAATCTAT TCTGAAGATA TCGATGCTCA GCTTCCATCT 
GAACTTTCTC GTCTTGGATT TAAATATCTC GTTTCTAATT GCTTTGATGC TCCTGCCCCA 
CTCAAACAAT TCTTATGCAA TGTCAGCGGT TTGAATAGAA TTCCATTCGG AGGATCTGAT 
GTTGAGAAAA AGATCGCTGA AGTATCGCAA GCGATCGAAC AAACGAGATA TTCAGTTTTT 
TTGACAGCCA ATATTAGATG TCAAAACTCG AAATCCAGAT ATGCAAATAA CACTCTTCAA 
TCTCAATCGG CCACACGCGA AGCAAATACA TGGCGAGACC AGTTCTTCAA AGTTTCTCCA 
GTTGCGAAGA GAACCGACAA CAGTATTCTT GAAGAAATTC AAAAACTGAA AGCAGAAATC 
GATATTCGAT CTGAACAACT ACGAGAGAAA CGTGGAGCAA TTCAGAAGGA ACGAGATGTT 
TTACGTCAAG AGCAAATGCA ATGGAAGTCG AAGAAACAAG TTCATACTAA ATGGAAAACA 
GAATTGGCGA GTGAAATGGC AAAATTGGAA GCTCTCGAAA ATGAAGTTGT TGATATTTCT 
GCAATTGAAG AAGAGTATGC GAATGTGGAA AAGAAGGCGA TTCTTGAGAC GAAAAAGATG 
CTGGAGAACA GTATTCGGTG GCATAAAGAA ATCATCGACA AGCATCGTTT AATTGGAATT 
TTCGAGCTGA GTGAATCCAT ATGCAAATCG AGAGTGAATA AATCAAATTC GGAAGCTGAA 
ACCCATCGAT CGAAGCTTGA AGATTTGAAA AGCGTCAAAG ATGCTGCTGA AGATCTACTG 
AAGACTGCGT TGAATCACAA GAAAGCAGCA GCAAGTGCAT TGATGAAGGA ATGTTCTTTG 
AAAACTTTAG ACGAATCGAA AATGAGTCCC GCTGAAAATA AGATTTATTC ATCTTTAGTT 
AAAATGTTCG AGGAAGCTGA TGTTCCGACT GATATGAATA CTCTGGATCA AGCAATCACT 
TCAGAGAAAA CCCGTTTGAA ACTTGCTGAA GATAGCGGCG AAGATGGTTC AATCGTGCAT 
GAACAGAGAT TAAAAGTTCT TGATGATGAT CTCGTTTTGG AAAAAACGCG TCAAGAGAAG 
CTTATTGAAA ACCGTGCACG TATCCACGAT AAACTAGGTG ATGAGATCAA CAATTGGCGT 
AAGGAAGTTG AGACAATGAT TGAACAAATC AATGTAAACT ACGTTCAATT CTTTGATTCT 
CTTGGTTGTC GCGGAGAAGT TAGTCTTGAA GTTCCCGAAA ATCCACTGGA TATTGAAAAA 
TATGGAATCA TGATTATGGT GTGCTTCCGC AAAGGAGAAA GTATGAAACG TCTTGACAAT 
AAAGTTCAAT CTGGAGGTGA ACGAAGTGTG GCTACGATGC TTTATCTTCT GGCTCTACAA 
CAATTATGCC CAGTTCCATT CAGATGTATT GATGAGATTA ATCAGGGAAT GGATCCTACA 
AACGAACGGA AAGTGTTTGA TATTATGGTT GGAATGTGGA ATGGAACCAC TGGAACACTT 
TCCAAAACTC AATACTTTTT GCTCTCTCCA AAACTTCTTC ATGGTTTGGA TATGCGCGAA 
AATGTGAACA TTGTTATGGT GAATAGTACA CTCACCAATT CTCATGGAAA GCATTACGAC 
ACCAGTGCCA AGATAGATGC GACATTTGCA AAAATGGGAA TCAGTGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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smc-5 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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