ORC6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ORC6
Entrez Gene ID	856518
Full Name	origin recognition complex subunit 6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179248.1	NP_011986.1	origin recognition complex subunit 6

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ABR135C	NP_983082.1
Kluyveromyces lactis	KLLA0F24112g	XP_456154.1
Saccharomyces cerevisiae (baker's yeast)	ORC6	NP_011986.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Orc6 RXL/cyclin-binding motif plays a positive role in the initial recruitment of Cdt1/Mcm2-7 to the origin, suggesting that this motif is critical for the switch between active and inhibited ORC function at the G1-to-S-phase transition.
Title: CDK prevents Mcm2-7 helicase loading by inhibiting Cdt1 interaction with Orc6.

In this study, yeast two-hybrid analysis was used to examine the interaction between Cdt1p and every ORC subunit; potent interaction with Orc6p, and weaker interaction with Orc2p and Orc5p was observed.
Title: Interaction between ORC and Cdt1p of Saccharomyces cerevisiae.

Orc6 is required for dynamic recruitment of Cdt1 during repeated loading of DNA helicases.
Title: Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading.

Orc6 was required for entry into S phase after pre-replicative complexes formation
Title: An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes.

The 'hydrophobic patch' (HP) of the Saccharomyces cerevisiae S-phase cyclin Clb5 facilitates its interaction with Orc6 (via its Cy or RXL motif), providing a mechanism that helps prevent rereplication from individual origins.
Title: Two-faced cyclins with eyes on the targets.

The following ORC6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ORC6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05316	NM_001179248.1 Latest version!	Saccharomyces cerevisiae S288C origin recognition complex subunit 6 (ORC6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ORC6 ( NM_001179248.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011986.1 Saccharomyces cerevisiae S288C origin recognition complex subunit 6 (ORC6), partial mRNA.

Price & Availability↑

CloneID	OSi05316
Clone ID Related Accession (Same CDS sequence)	NM_001179248.1
Accession Version	NM_001179248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	origin recognition complex subunit 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCATGC AACAAGTCCA ACATTGTGTC GCAGAAGTAC TTCGACTAGA TCCACAAGAA 
AAACCGGACT GGTCGAGCGG ATATTTGAAG AAGTTGACTA ATGCGACATC GATTTTATAT 
AATACTTCAC TGAACAAGGT AATGCTGAAA CAAGATGAAG AGGTTGCTAG ATGTCACATA 
TGTGCATACA TAGCGTCACA GAAAATGAAT GAAAAACACA TGCCTGACCT TTGCTATTAT 
ATAGACAGTA TTCCCTTGGA GCCGAAAAAA GCCAAGCATT TAATGAACCT TTTCAGACAA 
AGTTTATCTA ATTCTTCACC TATGAAACAA TTTGCTTGGA CACCGAGCCC CAAAAAGAAC 
AAACGCAGTC CAGTAAAGAA CGGTGGGAGG TTTACTTCTT CTGATCCGAA AGAGTTGAGG 
AATCAACTGT TTGGTACACC AACTAAAGTT AGGAAAAGCC AAAATAATGA TTCGTTCGTA 
ATACCAGAAC TACCCCCCAT GCAAACCAAT GAATCGCCGT CTATTACTAG GAGAAAGTTA 
GCATTTGAAG AGGATGAGGA TGAGGATGAA GAGGAACCAG GAAACGACGG TTTGTCTTTA 
AAAAGCCATA GTAATAAGAG CATTACTGGA ACCAGAAATG TAGATTCTGA TGAGTATGAA 
AACCATGAAA GTGACCCTAC AAGTGAGGAA GAGCCATTAG GTGTGCAAGA AAGCAGAAGC 
GGGAGAACGA AACAAAATAA GGCAGTTGGA AAACCGCAAT CAGAATTGAA GACGGCAAAA 
GCCCTGAGGA AAAGGGGCAG AATACCAAAT TCTTTGTTAG TAAAGAAGTA TTGCAAAATG 
ACTACTGAAG AAATAATACG GCTTTGCAAC GATTTTGAAT TACCAAGAGA AGTAGCATAT 
AAAATTGTGG ATGAGTACAA CATAAACGCG TCAAGATTGG TTTGCCCATG GCAATTAGTG 
TGTGGGTTAG TATTAAATTG TACATTCATT GTATTTAATG AAAGAAGACG CAAGGATCCA 
AGAATTGACC ATTTTATAGT CAGTAAGATG TGCAGCTTGA TGTTGACGTC AAAAGTGGAT 
GATGTTATTG AATGTGTAAA ATTAGTGAAG GAATTAATTA TCGGTGAAAA ATGGTTCAGA 
GATTTGCAAA TTAGGTATGA TGATTTTGAT GGCATCAGAT ACGATGAAAT TATATTTAGG 
AAACTGGGAT CGATGTTACA AACCACCAAT ATTTTGGTCA CAGACGACCA GTACAATATT 
TGGAAGAAAA GAATTGAAAT GGATTTGGCA TTAACAGAAC CTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011986.1
CDS	1..1308
Translation	MSMQQVQHCVAEVLRLDPQEKPDWSSGYLKKLTNATSILYNTSLNKVMLKQDEEVARCHICAYIASQKMNEKHMPDLCYYIDSIPLEPKKAKHLMNLFRQSLSNSSPMKQFAWTPSPKKNKRSPVKNGGRFTSSDPKELRNQLFGTPTKVRKSQNNDSFVIPELPPMQTNESPSITRRKLAFEEDEDEDEEEPGNDGLSLKSHSNKSITGTRNVDSDEYENHESDPTSEEEPLGVQESRSGRTKQNKAVGKPQSELKTAKALRKRGRIPNSLLVKKYCKMTTEEIIRLCNDFELPREVAYKIVDEYNINASRLVCPWQLVCGLVLNCTFIVFNERRRKDPRIDHFIVSKMCSLMLTSKVDDVIECVKLVKELIIGEKWFRDLQIRYDDFDGIRYDEIIFRKLGSMLQTTNILVTDDQYNIWKKRIEMDLALTEPL

Target ORF information:

RefSeq Version	NM_001179248.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C origin recognition complex subunit 6 (ORC6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179248.1

ORF Insert Sequence:

ATGTCCATGC AACAAGTCCA ACATTGTGTC GCAGAAGTAC TTCGACTAGA TCCACAAGAA 
AAACCGGACT GGTCGAGCGG ATATTTGAAG AAGTTGACTA ATGCGACATC GATTTTATAT 
AATACTTCAC TGAACAAGGT AATGCTGAAA CAAGATGAAG AGGTTGCTAG ATGTCACATA 
TGTGCATACA TAGCGTCACA GAAAATGAAT GAAAAACACA TGCCTGACCT TTGCTATTAT 
ATAGACAGTA TTCCCTTGGA GCCGAAAAAA GCCAAGCATT TAATGAACCT TTTCAGACAA 
AGTTTATCTA ATTCTTCACC TATGAAACAA TTTGCTTGGA CACCGAGCCC CAAAAAGAAC 
AAACGCAGTC CAGTAAAGAA CGGTGGGAGG TTTACTTCTT CTGATCCGAA AGAGTTGAGG 
AATCAACTGT TTGGTACACC AACTAAAGTT AGGAAAAGCC AAAATAATGA TTCGTTCGTA 
ATACCAGAAC TACCCCCCAT GCAAACCAAT GAATCGCCGT CTATTACTAG GAGAAAGTTA 
GCATTTGAAG AGGATGAGGA TGAGGATGAA GAGGAACCAG GAAACGACGG TTTGTCTTTA 
AAAAGCCATA GTAATAAGAG CATTACTGGA ACCAGAAATG TAGATTCTGA TGAGTATGAA 
AACCATGAAA GTGACCCTAC AAGTGAGGAA GAGCCATTAG GTGTGCAAGA AAGCAGAAGC 
GGGAGAACGA AACAAAATAA GGCAGTTGGA AAACCGCAAT CAGAATTGAA GACGGCAAAA 
GCCCTGAGGA AAAGGGGCAG AATACCAAAT TCTTTGTTAG TAAAGAAGTA TTGCAAAATG 
ACTACTGAAG AAATAATACG GCTTTGCAAC GATTTTGAAT TACCAAGAGA AGTAGCATAT 
AAAATTGTGG ATGAGTACAA CATAAACGCG TCAAGATTGG TTTGCCCATG GCAATTAGTG 
TGTGGGTTAG TATTAAATTG TACATTCATT GTATTTAATG AAAGAAGACG CAAGGATCCA 
AGAATTGACC ATTTTATAGT CAGTAAGATG TGCAGCTTGA TGTTGACGTC AAAAGTGGAT 
GATGTTATTG AATGTGTAAA ATTAGTGAAG GAATTAATTA TCGGTGAAAA ATGGTTCAGA 
GATTTGCAAA TTAGGTATGA TGATTTTGAT GGCATCAGAT ACGATGAAAT TATATTTAGG 
AAACTGGGAT CGATGTTACA AACCACCAAT ATTTTGGTCA CAGACGACCA GTACAATATT 
TGGAAGAAAA GAATTGAAAT GGATTTGGCA TTAACAGAAC CTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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ORC6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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