SEC12 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SEC12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04649 NM_001183203.1
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Saccharomyces cerevisiae S288C Sar family guanine nucleotide exchange factor SEC12 (SEC12), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04649
    Clone ID Related Accession (Same CDS sequence) NM_001183203.1
    Accession Version NM_001183203.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Sar family guanine nucleotide exchange factor SEC12
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAGTTCG TGACAGCTAG TTATAACGTC GGGTATCCTG CGTACGGTGC AAAATTTTTG 
    AATAACGACA CATTACTTGT GGCAGGCGGT GGAGGAGAAG GAAACAATGG CATACCAAAC
    AAGCTGACGG TCTTGCGCGT GGATCCTACC AAAGATACTG AGAAGGAACA GTTTCATATA
    TTGAGCGAGT TTGCATTGGA AGACAACGAC GACTCTCCTA CTGCAATTGA CGCTTCCAAG
    GGTATCATTT TGGTTGGCTG CAATGAAAAT AGCACTAAGA TTACCCAAGG TAAAGGTAAT
    AAGCACTTGA GAAAATTTAA ATACGATAAA GTGAATGATC AATTGGAGTT CCTCACTAGT
    GTAGACTTTG ACGCATCTAC AAATGCGGAT GACTACACGA AGCTGGTTTA TATTTCACGA
    GAAGGTACCG TTGCAGCTAT CGCATCATCT AAAGTACCTG CTATAATGAG AATCATTGAC
    CCGAGCGACT TGACAGAGAA GTTTGAGATC GAGACTAGGG GTGAAGTAAA GGATTTACAC
    TTTTCCACTG ATGGTAAGGT TGTTGCTTAT ATCACCGGTT CTAGCTTGGA AGTGATTTCA
    ACAGTGACTG GAAGTTGCAT TGCTAGGAAA ACAGATTTTG ATAAGAATTG GAGTTTATCT
    AAAATAAACT TCATAGCCGA TGACACAGTA TTGATAGCAG CCTCTTTAAA AAAAGGGAAA
    GGTATTGTGC TGACCAAAAT AAGCATCAAA TCAGGAAACA CTTCCGTATT AAGATCCAAA
    CAAGTGACAA ACAGATTCAA AGGGATTACT TCTATGGATG TCGACATGAA GGGTGAATTG
    GCGGTACTGG CAAGTAATGA CAATTCCATA GCTCTTGTGA AACTAAAAGA CCTGTCAATG
    TCTAAAATAT TCAAACAAGC TCATAGTTTT GCCATTACAG AGGTCACTAT CTCTCCGGAC
    TCTACATATG TGGCGAGTGT TTCGGCAGCC AACACTATCC ACATAATAAA ATTACCGCTT
    AACTACGCCA ACTACACCTC AATGAAACAA AAAATCTCTA AATTTTTCAC CAACTTCATC
    CTTATTGTGC TGCTTTCTTA CATTTTACAG TTCTCCTATA AGCACAATTT GCATTCCATG
    CTTTTCAATT ACGCGAAGGA CAATTTTCTA ACGAAAAGAG ACACCATCTC TTCGCCCTAC
    GTAGTTGATG AAGACTTACA TCAAACAACT TTGTTTGGCA ACCACGGTAC AAAAACATCT
    GTACCTAGCG TAGATTCCAT AAAAGTGCAT GGCGTGCATG AGACGAGTTC TGTGAATGGA
    ACTGAAGTCT TATGTACTGA AAGTAACATT ATTAATACTG GAGGGGCAGA GTTTGAGATC
    ACCAACGCAA CTTTTCGAGA AATAGATGAT GCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014423.1
    CDS1..1416
    Translation

    Target ORF information:

    RefSeq Version NM_001183203.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Sar family guanine nucleotide exchange factor SEC12 (SEC12), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001183203.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAGTTCG TGACAGCTAG TTATAACGTC GGGTATCCTG CGTACGGTGC AAAATTTTTG 
    AATAACGACA CATTACTTGT GGCAGGCGGT GGAGGAGAAG GAAACAATGG CATACCAAAC
    AAGCTGACGG TCTTGCGCGT GGATCCTACC AAAGATACTG AGAAGGAACA GTTTCATATA
    TTGAGCGAGT TTGCATTGGA AGACAACGAC GACTCTCCTA CTGCAATTGA CGCTTCCAAG
    GGTATCATTT TGGTTGGCTG CAATGAAAAT AGCACTAAGA TTACCCAAGG TAAAGGTAAT
    AAGCACTTGA GAAAATTTAA ATACGATAAA GTGAATGATC AATTGGAGTT CCTCACTAGT
    GTAGACTTTG ACGCATCTAC AAATGCGGAT GACTACACGA AGCTGGTTTA TATTTCACGA
    GAAGGTACCG TTGCAGCTAT CGCATCATCT AAAGTACCTG CTATAATGAG AATCATTGAC
    CCGAGCGACT TGACAGAGAA GTTTGAGATC GAGACTAGGG GTGAAGTAAA GGATTTACAC
    TTTTCCACTG ATGGTAAGGT TGTTGCTTAT ATCACCGGTT CTAGCTTGGA AGTGATTTCA
    ACAGTGACTG GAAGTTGCAT TGCTAGGAAA ACAGATTTTG ATAAGAATTG GAGTTTATCT
    AAAATAAACT TCATAGCCGA TGACACAGTA TTGATAGCAG CCTCTTTAAA AAAAGGGAAA
    GGTATTGTGC TGACCAAAAT AAGCATCAAA TCAGGAAACA CTTCCGTATT AAGATCCAAA
    CAAGTGACAA ACAGATTCAA AGGGATTACT TCTATGGATG TCGACATGAA GGGTGAATTG
    GCGGTACTGG CAAGTAATGA CAATTCCATA GCTCTTGTGA AACTAAAAGA CCTGTCAATG
    TCTAAAATAT TCAAACAAGC TCATAGTTTT GCCATTACAG AGGTCACTAT CTCTCCGGAC
    TCTACATATG TGGCGAGTGT TTCGGCAGCC AACACTATCC ACATAATAAA ATTACCGCTT
    AACTACGCCA ACTACACCTC AATGAAACAA AAAATCTCTA AATTTTTCAC CAACTTCATC
    CTTATTGTGC TGCTTTCTTA CATTTTACAG TTCTCCTATA AGCACAATTT GCATTCCATG
    CTTTTCAATT ACGCGAAGGA CAATTTTCTA ACGAAAAGAG ACACCATCTC TTCGCCCTAC
    GTAGTTGATG AAGACTTACA TCAAACAACT TTGTTTGGCA ACCACGGTAC AAAAACATCT
    GTACCTAGCG TAGATTCCAT AAAAGTGCAT GGCGTGCATG AGACGAGTTC TGTGAATGGA
    ACTGAAGTCT TATGTACTGA AAGTAACATT ATTAATACTG GAGGGGCAGA GTTTGAGATC
    ACCAACGCAA CTTTTCGAGA AATAGATGAT GCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG