PRP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP2
Entrez Gene ID	855745
Full Name	DEAH-box RNA-dependent ATPase PRP2
Synonyms	RNA2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183188.1	NP_014408.1	DEAH-box RNA-dependent ATPase PRP2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PRP2	NP_014408.1
Kluyveromyces lactis	KLLA0F06204g	XP_455361.1
Eremothecium gossypii	AGOS_ADR287C	NP_984383.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spp2 plays a major role in coupling Prp2's ATPase activity to remodeling of the spliceosome into a catalytically active machine
Title: The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome.

NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome.
Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.

propose that Prp2 is recruited to the spliceosome via interaction with Brr2 and is spatially positioned to interact with this specific region of the pre-mRNA, which stimulates the ATPase activity of Prp2 to promote progression of the first catalytic step
Title: The interaction of Prp2 with a defined region of the intron is required for the first splicing reaction.

The results suggest high cooperativity of multiple Prp2-mediated structural rearrangements at the spliceosome's catalytic core.
Title: Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.

In the absence of Cwc22, Prp2 can bind to the spliceosome but is dissociated upon ATP hydrolysis without promoting the release of SF3a/b.
Title: Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway.

The following PRP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04635	NM_001183188.1 Latest version!	Saccharomyces cerevisiae S288C DEAH-box RNA-dependent ATPase PRP2 (PRP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP2 ( NM_001183188.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014408.1 Saccharomyces cerevisiae S288C DEAH-box RNA-dependent ATPase PRP2 (PRP2), partial mRNA.

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CloneID	OSi04635
Clone ID Related Accession (Same CDS sequence)	NM_001183188.1
Accession Version	NM_001183188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DEAH-box RNA-dependent ATPase PRP2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAAGTA TTACATCTGA AACCGGTAAG CGAAGAGTGA AACGAACGTA TGAAGTTACA 
AGACAGAATG ACAATGCAGT AAGAATAGAG CCTTCTTCCT TGGGTGAGGA AGAAGACAAA 
GAGGCCAAGG ATAAGAACTC TGCCTTGCAG CTAAAACGAT CACGCTATGA CCCCAATAAA 
GTTTTTTCTA ATACGAACCA AGGTCCAGAA AAGAACAATT TAAAAGGAGA ACAACTTGGA 
TCACAAAAAA AGTCATCAAA GTATGATGAA AAAATAACGT CAAATAATGA ACTTACTACA 
AAGAAGGGTC TACTAGGTGA CTCCGAGAAT GAAACTAAGT ACGCCTCTTC TAATTCTAAA 
TTTAACGTAG AAGTTACTCA TAAAATAAAG AATGCAAAAG AAATTGACAA AATAAACAGA 
CAACGCATGT GGGAAGAACA ACAATTGCGA AATGCTATGG CGGGGCAAAG CGATCACCCT 
GATGATATAA CATTGGAAGG CTCTGACAAG TACGATTATG TGTTCGACAC AGATGCAATG 
ATTGATTATA CTAATGAGGA GGACGATTTA CTACCTGAAG AGAAACTTCA ATATGAGGCA 
CGTCTAGCAC AAGCTTTAGA AACTGAGGAA AAGCGTATAC TGACTATACA AGAGGCAAGA 
AAACTTTTGC CTGTACATCA ATACAAGGAT GAGCTACTAC AAGAGATCAA AAAAAATCAA 
GTTTTGATTA TAATGGGTGA AACCGGTTCT GGTAAAACCA CACAGTTACC CCAATATTTG 
GTAGAAGATG GATTCACTGA TCAGGGAAAG TTACAGATTG CCATAACACA ACCACGTCGT 
GTGGCGGCCA CTTCTGTTGC TGCAAGAGTA GCAGATGAAA TGAATGTTGT GCTTGGGAAG 
GAAGTGGGTT ATCAGATCCG TTTTGAAGAC AAAACAACCC CAAATAAAAC GGTTCTAAAG 
TATATGACGG ATGGTATGTT ACTAAGAGAA TTTCTTACAG ATTCCAAATT AAGCAAATAT 
TCTTGTATTA TGATTGATGA AGCTCATGAA CGTACTCTAG CCACAGATAT CCTGATAGGT 
TTGCTGAAAG ATATTTTGCC GCAGAGGCCC ACTTTAAAAT TGCTTATATC ATCAGCAACA 
ATGAACGCAA AAAAATTTTC AGAGTTTTTT GATAATTGTC CCATTTTCAA CGTTCCTGGG 
AGAAGATATC CTGTAGATAT CCACTACACA TTACAGCCGG AGGCAAACTA TATACATGCC 
GCTATAACAA CTATTTTTCA AATTCATACT ACGCAAAGTT TACCGGGGGA TATATTGGTG 
TTTTTAACGG GGCAAGAAGA AATTGAAAGA ACAAAAACCA AACTGGAGGA GATCATGTCT 
AAATTAGGTT CTAGAACAAA ACAAATGATT ATAACTCCTA TATACGCAAA TTTACCCCAA 
GAGCAGCAAT TGAAAATTTT CCAGCCGACT CCGGAAAATT GTAGGAAAGT TGTTTTGGCA 
ACTAATATTG CGGAAACTTC ATTGACCATT GATGGTATTA GATATGTCAT TGATCCTGGA 
TTTGTTAAGG AAAATTCGTA CGTACCCTCC ACTGGAATGA CCCAACTTTT AACAGTTCCA 
TGCTCAAGAG CTTCAGTTGA TCAGCGTGCC GGTAGGGCTG GTCGTGTTGG GCCGGGAAAA 
TGTTTTAGAA TTTTTACCAA ATGGTCATAT TTGCACGAGT TGGAACTTAT GCCTAAGCCT 
GAGATCACAA GAACAAATCT TTCTAATACG GTGTTATTAT TATTATCGCT TGGCGTTACT 
GACCTAATAA AGTTTCCCCT GATGGATAAG CCCAGTATCC CCACATTACG GAAGTCTTTG 
GAAAATTTAT ACATTTTAGG TGCCTTAAAT AGCAAAGGGA CAATCACTCG GTTAGGTAAA 
ATGATGTGCG AGTTTCCTTG TGAACCTGAA TTTGCAAAAG TGCTTTATAC AGCTGCTACG 
CACGAACAAT GTCAAGGTGT ACTAGAAGAG TGTTTGACCA TAGTTTCGAT GCTACATGAA 
ACACCTTCAT TATTTATTGG TCAAAAAAGA GATGCTGCGG CAAGTGTGTT AAGCGAAGTG 
GAAAGTGATC ACATATTGTA TTTGGAAATC TTCAACCAAT GGAGAAACTC TAAATTTTCC 
AGAAGTTGGT GTCAAGACCA CAAAATTCAA TTCAAAACGA TGTTGAGGGT AAGAAATATT 
AGAAACCAAT TATTCAGGTG CAGTGAAAAG GTTGGATTAG TTGAAAAGAA CGACCAAGCC 
AGGATGAAGA TAGGCAATAT AGCGGGCTAC ATAAATGCTA GAATAACTCG ATGCTTCATC 
AGCGGGTTTC CGATGAATAT TGTACAATTA GGCCCCACCG GCTATCAAAC CATGGGTAGG 
TCATCAGGTG GGCTGAACGT TAGTGTTCAT CCTACCAGTA TACTTTTCGT TAATCATAAG 
GAAAAGGCGC AGAGACCAAG CAAATATGTG CTTTATCAAC AATTGATGCT AACGTCGAAG 
GAATTTATCA GGGACTGTCT AGTTATCCCT AAGGAAGAAT GGTTGATAGA CATGGTTCCA 
CAAATCTTTA AAGATTTAAT TGACGATAAA ACAAATAGGG GGAGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014408.1
CDS	1..2631
Translation	MSSITSETGKRRVKRTYEVTRQNDNAVRIEPSSLGEEEDKEAKDKNSALQLKRSRYDPNKVFSNTNQGPEKNNLKGEQLGSQKKSSKYDEKITSNNELTTKKGLLGDSENETKYASSNSKFNVEVTHKIKNAKEIDKINRQRMWEEQQLRNAMAGQSDHPDDITLEGSDKYDYVFDTDAMIDYTNEEDDLLPEEKLQYEARLAQALETEEKRILTIQEARKLLPVHQYKDELLQEIKKNQVLIIMGETGSGKTTQLPQYLVEDGFTDQGKLQIAITQPRRVAATSVAARVADEMNVVLGKEVGYQIRFEDKTTPNKTVLKYMTDGMLLREFLTDSKLSKYSCIMIDEAHERTLATDILIGLLKDILPQRPTLKLLISSATMNAKKFSEFFDNCPIFNVPGRRYPVDIHYTLQPEANYIHAAITTIFQIHTTQSLPGDILVFLTGQEEIERTKTKLEEIMSKLGSRTKQMIITPIYANLPQEQQLKIFQPTPENCRKVVLATNIAETSLTIDGIRYVIDPGFVKENSYVPSTGMTQLLTVPCSRASVDQRAGRAGRVGPGKCFRIFTKWSYLHELELMPKPEITRTNLSNTVLLLLSLGVTDLIKFPLMDKPSIPTLRKSLENLYILGALNSKGTITRLGKMMCEFPCEPEFAKVLYTAATHEQCQGVLEECLTIVSMLHETPSLFIGQKRDAAASVLSEVESDHILYLEIFNQWRNSKFSRSWCQDHKIQFKTMLRVRNIRNQLFRCSEKVGLVEKNDQARMKIGNIAGYINARITRCFISGFPMNIVQLGPTGYQTMGRSSGGLNVSVHPTSILFVNHKEKAQRPSKYVLYQQLMLTSKEFIRDCLVIPKEEWLIDMVPQIFKDLIDDKTNRGRR

Target ORF information:

RefSeq Version	NM_001183188.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DEAH-box RNA-dependent ATPase PRP2 (PRP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183188.1

ORF Insert Sequence:

ATGTCAAGTA TTACATCTGA AACCGGTAAG CGAAGAGTGA AACGAACGTA TGAAGTTACA 
AGACAGAATG ACAATGCAGT AAGAATAGAG CCTTCTTCCT TGGGTGAGGA AGAAGACAAA 
GAGGCCAAGG ATAAGAACTC TGCCTTGCAG CTAAAACGAT CACGCTATGA CCCCAATAAA 
GTTTTTTCTA ATACGAACCA AGGTCCAGAA AAGAACAATT TAAAAGGAGA ACAACTTGGA 
TCACAAAAAA AGTCATCAAA GTATGATGAA AAAATAACGT CAAATAATGA ACTTACTACA 
AAGAAGGGTC TACTAGGTGA CTCCGAGAAT GAAACTAAGT ACGCCTCTTC TAATTCTAAA 
TTTAACGTAG AAGTTACTCA TAAAATAAAG AATGCAAAAG AAATTGACAA AATAAACAGA 
CAACGCATGT GGGAAGAACA ACAATTGCGA AATGCTATGG CGGGGCAAAG CGATCACCCT 
GATGATATAA CATTGGAAGG CTCTGACAAG TACGATTATG TGTTCGACAC AGATGCAATG 
ATTGATTATA CTAATGAGGA GGACGATTTA CTACCTGAAG AGAAACTTCA ATATGAGGCA 
CGTCTAGCAC AAGCTTTAGA AACTGAGGAA AAGCGTATAC TGACTATACA AGAGGCAAGA 
AAACTTTTGC CTGTACATCA ATACAAGGAT GAGCTACTAC AAGAGATCAA AAAAAATCAA 
GTTTTGATTA TAATGGGTGA AACCGGTTCT GGTAAAACCA CACAGTTACC CCAATATTTG 
GTAGAAGATG GATTCACTGA TCAGGGAAAG TTACAGATTG CCATAACACA ACCACGTCGT 
GTGGCGGCCA CTTCTGTTGC TGCAAGAGTA GCAGATGAAA TGAATGTTGT GCTTGGGAAG 
GAAGTGGGTT ATCAGATCCG TTTTGAAGAC AAAACAACCC CAAATAAAAC GGTTCTAAAG 
TATATGACGG ATGGTATGTT ACTAAGAGAA TTTCTTACAG ATTCCAAATT AAGCAAATAT 
TCTTGTATTA TGATTGATGA AGCTCATGAA CGTACTCTAG CCACAGATAT CCTGATAGGT 
TTGCTGAAAG ATATTTTGCC GCAGAGGCCC ACTTTAAAAT TGCTTATATC ATCAGCAACA 
ATGAACGCAA AAAAATTTTC AGAGTTTTTT GATAATTGTC CCATTTTCAA CGTTCCTGGG 
AGAAGATATC CTGTAGATAT CCACTACACA TTACAGCCGG AGGCAAACTA TATACATGCC 
GCTATAACAA CTATTTTTCA AATTCATACT ACGCAAAGTT TACCGGGGGA TATATTGGTG 
TTTTTAACGG GGCAAGAAGA AATTGAAAGA ACAAAAACCA AACTGGAGGA GATCATGTCT 
AAATTAGGTT CTAGAACAAA ACAAATGATT ATAACTCCTA TATACGCAAA TTTACCCCAA 
GAGCAGCAAT TGAAAATTTT CCAGCCGACT CCGGAAAATT GTAGGAAAGT TGTTTTGGCA 
ACTAATATTG CGGAAACTTC ATTGACCATT GATGGTATTA GATATGTCAT TGATCCTGGA 
TTTGTTAAGG AAAATTCGTA CGTACCCTCC ACTGGAATGA CCCAACTTTT AACAGTTCCA 
TGCTCAAGAG CTTCAGTTGA TCAGCGTGCC GGTAGGGCTG GTCGTGTTGG GCCGGGAAAA 
TGTTTTAGAA TTTTTACCAA ATGGTCATAT TTGCACGAGT TGGAACTTAT GCCTAAGCCT 
GAGATCACAA GAACAAATCT TTCTAATACG GTGTTATTAT TATTATCGCT TGGCGTTACT 
GACCTAATAA AGTTTCCCCT GATGGATAAG CCCAGTATCC CCACATTACG GAAGTCTTTG 
GAAAATTTAT ACATTTTAGG TGCCTTAAAT AGCAAAGGGA CAATCACTCG GTTAGGTAAA 
ATGATGTGCG AGTTTCCTTG TGAACCTGAA TTTGCAAAAG TGCTTTATAC AGCTGCTACG 
CACGAACAAT GTCAAGGTGT ACTAGAAGAG TGTTTGACCA TAGTTTCGAT GCTACATGAA 
ACACCTTCAT TATTTATTGG TCAAAAAAGA GATGCTGCGG CAAGTGTGTT AAGCGAAGTG 
GAAAGTGATC ACATATTGTA TTTGGAAATC TTCAACCAAT GGAGAAACTC TAAATTTTCC 
AGAAGTTGGT GTCAAGACCA CAAAATTCAA TTCAAAACGA TGTTGAGGGT AAGAAATATT 
AGAAACCAAT TATTCAGGTG CAGTGAAAAG GTTGGATTAG TTGAAAAGAA CGACCAAGCC 
AGGATGAAGA TAGGCAATAT AGCGGGCTAC ATAAATGCTA GAATAACTCG ATGCTTCATC 
AGCGGGTTTC CGATGAATAT TGTACAATTA GGCCCCACCG GCTATCAAAC CATGGGTAGG 
TCATCAGGTG GGCTGAACGT TAGTGTTCAT CCTACCAGTA TACTTTTCGT TAATCATAAG 
GAAAAGGCGC AGAGACCAAG CAAATATGTG CTTTATCAAC AATTGATGCT AACGTCGAAG 
GAATTTATCA GGGACTGTCT AGTTATCCCT AAGGAAGAAT GGTTGATAGA CATGGTTCCA 
CAAATCTTTA AAGATTTAAT TGACGATAAA ACAAATAGGG GGAGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PRP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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