CYR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CYR1
Entrez Gene ID	853452
Full Name	adenylate cyclase
Synonyms	CDC35,FIL1,HSR1,SRA4,TSM0185
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181439.3	NP_012529.3	adenylate cyclase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F15708g	XP_455786.1
Eremothecium gossypii	AGOS_AAL162C	NP_982380.1
Saccharomyces cerevisiae (baker's yeast)	CYR1	NP_012529.3

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Snf1 Phosphorylates Adenylate Cyclase and Negatively Regulates Protein Kinase A-dependent Transcription in Saccharomyces cerevisiae
Title: Snf1 Phosphorylates Adenylate Cyclase and Negatively Regulates Protein Kinase A-dependent Transcription in Saccharomyces cerevisiae.

Adenylate cyclase activity does not influence the Ras2 activation state and is required for the proper interaction between Ras2 and the Ira proteins.
Title: Evidence for adenylate cyclase as a scaffold protein for Ras2-Ira interaction in Saccharomyces cerevisie.

Protein coding and expression variation of CYR1 enhances the formation of colony biofilms.
Title: The genetic architecture of biofilm formation in a clinical isolate of Saccharomyces cerevisiae.

Gpa2 relieves the inhibition imposed by the kelch-repeat proteins on Protein Kinase A (PKA), thereby bypassing adenylate cyclase for direct regulation of PKA.
Title: Kelch-repeat proteins interacting with the Galpha protein Gpa2 bypass adenylate cyclase for direct regulation of protein kinase A in yeast.

The following CYR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02697	NM_001181439.3 Latest version!	Saccharomyces cerevisiae S288C adenylate cyclase (CYR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYR1 ( NM_001181439.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012529.3 Saccharomyces cerevisiae S288C adenylate cyclase (CYR1), partial mRNA.

Price & Availability↑

CloneID	OSi02697
Clone ID Related Accession (Same CDS sequence)	NM_001181439.3
Accession Version	NM_001181439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	adenylate cyclase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181439.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATCAA AACCTGATAC TGGTTCGGAA ATTTCTGGCC CTCAGCGACA GGAAGAACAA 
GAACAACAGA TAGAGCAGAG CTCACCTACG GAAGCAAACG ATAGAAGCAT TCATGATGAG 
GTACCAAAAG TCAAGAAGCG TCACGAACAA AATAGTGGTC ACAAATCAAG AAGGAATAGC 
GCATATAGTT ATTACAGCCC ACGGTCGCTT TCTATGACCA AAAGCAGGGA GAGTATCACT 
CCAAATGGTA TGGATGATGT AAGTATTTCG AACGTGGAAC ATCCAAGGCC GACAGAACCG 
AAAATCAAAA GGGGTCCATA TTTACTGAAG AAAACATTGA GCAGTCTTTC AATGACGAGC 
GCGAATAGTA CTCATGATGA TAATAAAGAC CACGGTTACG CTTTGAATTC ATCCAAGACG 
CACAACTACA CATCTACTCA TAACCATCAT GACGGTCATC ATGATCATCA TCATGTTCAG 
TTTTTTCCCA ATAGGAAGCC ATCATTAGCG GAAACCCTAT TCAAAAGGTT TTCAGGGTCA 
AACAGTCACG ATGGCAATAA GTCAGGAAAG GAAAGTAAAG TTGCTAACCT TTCCCTTTCA 
ACGGTAAATC CTGCACCTGC TAATAGGAAA CCTTCTAAAG ACTCCACTTT ATCTAATCAC 
TTGGCTGATA ACGTGCCAAG CACTTTACGA AGGAAAGTGT CCTCATTGGT ACGTGGTTCT 
TCCGTCCATG ATATAAATAA TGGTATTGCA GATAAACAGA TTAGACCAAA GGCTGTTGCG 
CAATCAGAAA ATACATTACA TTCATCCGAT GTTCCCAATA GCAAACGCTC GCACAGAAAA 
AGCTTTCTGC TAGGCTCCAC ATCTTCTTCA AGCAGTAGAA GAGGTTCAAA TGTCAGTTCA 
ATGACTAACA GTGACAGTGC AAGTATGGCG ACGTCGGGTA GTCATGTTCT CCAACATAAC 
GTATCTAATG TTTCTCCAAC TACTAAAAGT AAGGACAGCG TTAACAGCGA ATCCGCCGAT 
CACACTAATA ATAAATCCGA GAAAGTGACT CCAGAATATA ATGAGAACAT TCCGGAAAAT 
TCTAACTCTG ACAACAAACG CGAAGCCACA ACGCCTACTA TAGAAACACC CATTTCATGT 
AAACCATCCC TTTTCAGGCT AGATACAAAC CTTGAGGATG TTACTGATAT TACAAAGACG 
GTGCCACCCA CCGCTGTCAA TTCTACACTA AATTCTACAC ACGGGACTGA GACTGCCTCA 
CCCAAAACGG TGATCATGCC TGAAGGTCCT AGGAAGTCGG TGTCAATGGC TGATCTCTCC 
GTCGCTGCCG CAGCACCTAA TGGTGAATTC ACATCAACTT CCAATGATAG ATCACAATGG 
GTAGCACCTC AAAGCTGGGA TGTGGAAACC AAAAGGAAAA AAACAAAACC TAAAGGGAGA 
TCGAAATCAA GAAGGTCAAG TATAGATGCT GATGAACTTG ATCCCATGTC ACCGGGGCCA 
CCTTCAAAAA AAGACTCTCG TCATCATCAC GATCGAAAGG ATAACGAATC AATGGTCACT 
GCGGGTGACA GTAACTCAAG TTTTGTTGAT ATATGTAAAG AAAACGTTCC GAATGATAGC 
AAGACCGCAC TCGATACTAA ATCTGTGAAC CGCTTAAAAA GTAATTTGGC TATGAGTCCC 
CCAAGTATAC GATATGCTCC ATCAAATTTA GATGGGGACT ACGACACGTC TTCCACTTCC 
TCATCTTTAC CGTCCTCATC TATTAGTTCA GAAGATACAT CTTCCTGCAG CGATTCCTCT 
TCGTACACTA ACGCGTATAT GGAGGCCAAC CGAGAGCAGG ATAATAAAAC ACCGATCCTG 
AATAAAACGA AATCGTATAC CAAGAAATTT ACATCCTCTT CGGTAAATAT GAATTCACCA 
GATGGTGCCC AGAGTTCTGG ATTATTACTA CAAGATGAGA AGGACGATGA GGTCGAGTGC 
CAACTGGAAC ATTACTATAA AGATTTCAGT GATTTAGATC CAAAGAGGCA CTATGCTATT 
CGTATATTCA ATACTGATGA CACTTTTACG ACTCTCTCAT GTACTCCAGC GACTACCGTC 
GAAGAGATAA TACCTGCACT TAAAAGAAAA TTTAACATTA CAGCGCAAGG GAATTTTCAA 
ATTTCCCTGA AGGTGGGAAA GTTGTCAAAA ATTTTGAGAC CAACTTCGAA ACCTATTTTA 
ATTGAAAGAA AACTTTTACT TTTGAATGGT TATCGAAAGT CAGACCCACT TCATATTATG 
GGTATAGAGG ATTTAAGTTT TGTTTTTAAG TTTCTTTTCC ATCCTGTCAC ACCTTCTCAC 
TTTACTCCTG AACAAGAACA AAGAATAATG AGAAGCGAAT TTGTTCACGT AGATTTAAGG 
AATATGGATC TGACTACACC TCCCATCATT TTTTACCAGC ATACGTCAGA AATAGAAAGT 
TTAGACGTTT CTAATAACGC AAATATATTC CTACCTCTGG AGTTCATTGA AAGCTCGATT 
AAATTATTAA GTTTGAGAAT GGTTAATATT AGAGCATCTA AATTTCCTTC CAATATCACT 
AAGGCGTATA AACTAGTATC TTTGGAATTA CAGAGAAACT TCATAAGAAA AGTACCGAAC 
TCAATCATGA AACTGAGTAA TTTAACGATA TTAAACCTTC AATGTAATGA GCTTGAAAGC 
CTACCGGCTG GATTTGTTGA ACTGAAAAAT CTGCAATTGC TAGACTTGTC TTCAAACAAG 
TTCATGCACT ACCCAGAAGT TATTAACTAC TGCACCAATC TTTTACAAAT AGACCTATCA 
TATAATAAAA TCCAAAGCTT ACCACAGTCC ACTAAGTACC TAGTAAAGCT TGCGAAGATG 
AACCTTTCTC ATAACAAACT AAATTTTATA GGCGACTTAT CGGAAATGAC AGATTTGAGG 
ACGCTGAACC TAAGATATAA CAGAATATCA TCAATTAAGA CAAATGCGTC TAACTTGCAG 
AACCTTTTTT TAACAGATAA TAGAATTTCG AACTTTGAAG ACACTTTGCC GAAACTAAGA 
GCCCTTGAAA TTCAAGAGAA TCCAATCACT TCTATATCCT TCAAAGATTT TTATCCAAAA 
AACATGACAA GTTTGACGTT GAACAAGGCA CAGTTATCGA GTATTCCTGG AGAATTACTC 
ACCAAACTAT CTTTCCTCGA GAAACTTGAA CTTAATCAGA ATAATTTGAC TAGACTGCCA 
CAGGAGATAT CCAAGTTGAC TAAATTAGTT TTCCTTTCAG TGGCGAGAAA CAAACTAGAG 
TATATTCCAC CCGAGCTATC TCAACTGAAA AGTTTGAGGA CATTAGATCT ACATTCTAAC 
AACATAAGGG ACTTTGTTGA CGGTATGGAA AACCTTGAAC TAACATCGCT AAATATTTCA 
TCGAATGCAT TCGGTAACTC TAGCTTAGAA AATTCTTTTT ACCATAACAT GTCATATGGG 
TCAAAGTTAT CTAAAAGCCT GATGTTTTTT ATTGCTGCAG ACAATCAATT TGATGATGCT 
ATGTGGCCTC TTTTCAATTG CTTTGTCAAT CTGAAAGTGC TAAATCTTTC TTACAACAAT 
TTTTCAGATG TATCGCACAT GAAACTTGAG AGCATTACCG AATTGTACCT CTCCGGTAAT 
AAGCTCACGA CATTGTCGGG TGATACAGTT TTGAAATGGA GCTCTTTAAA GACTTTAATG 
TTGAATAGTA ACCAAATGTT ATCTCTGCCT GCAGAATTAT CAAATCTCTC ACAGCTAAGT 
GTATTTGATG TTGGAGCAAA TCAATTAAAG TATAATATAT CAAACTATCA TTACGATTGG 
AACTGGAGGA ATAATAAAGA ACTAAAATAT TTGAATTTTT CAGGAAATCG AAGGTTTGAA 
ATAAAGTCAT TTATAAGTCA CGATATTGAT GCTGATTTGT CAGATCTGAC AGTATTACCT 
CAGTTAAAGG TACTAGGTTT AATGGACGTA ACTTTAAATA CTACCAAAGT ACCGGATGAA 
AATGTCAATT TCCGTTTAAG GACAACTGCA TCAATAATAA ATGGGATGCG CTACGGTGTT 
GCTGATACAT TAGGTCAAAG AGACTATGTG TCATCTCGTG ATGTTACCTT TGAAAGATTC 
CGCGGAAATG ACGACGAATG CTTACTATGT CTTCATGATA GTAAAAACCA AAATGCAGAT 
TATGGCCACA ATATATCAAG AATTGTTAGA GATATTTACG ATAAAATACT GATCAGACAA 
CTGGAAAGGT ATGGAGACGA AACAGATGAT AATATAAAAA CTGCACTTCG TTTCAGTTTT 
TTGCAACTGA ATAAGGAGAT TAACGGAATG CTAAATTCTG TTGATAATGG TGCCGATGTT 
GCCAATCTTT CATATGCAGA CTTGCTAAGT GGCGCTTGCT CTACTGTGAT ATATATCAGA 
GGGAAGAAAC TCTTCGCTGC AAATTTAGGT GACTGTATGG CTATTTTATC CAAAAACAAT 
GGTGACTACC AAACGCTAAC CAAACAACAT CTCCCAACAA AGCGGGAAGA ATACGAGAGG 
ATCAGAATAT CTGGCGGGTA TGTCAACAAT GGAAAATTAG ATGGTGTTGT AGATGTGTCT 
AGAGCAGTGG GTTTTTTTGA TTTGCTTCCC CACATTCATG CTTCTCCCGA CATATCTGTC 
GTGACATTAA CAAAAGCAGA CGAGATGCTT ATTGTAGCAA CGCATAAGTT ATGGGAATAC 
ATGGACGTGG ATACAGTTTG TGATATCGCG CGTGAGAATA GTACTGATCC ACTCCGTGCC 
GCAGCTGAGT TGAAGGATCA TGCCATGGCT TACGGCTGTA CAGAGAATAT TACAATTTTG 
TGCCTTGCTC TTTACGAGAA CATTCAGCAA CAAAATCGGT TCACTTTAAA TAAAAACTCT 
TTAATGACTA GAAGAAGTAC TTTCGAGGAT ACTACATTAA GAAGACTTCA ACCTGAGATT 
TCTCCGCCAA CAGGTAACCT AGCAATGGTC TTCACTGATA TCAAAAGCTC AACCTTCTTA 
TGGGAGCTAT TCCCTAACGC AATGAGGACC GCAATAAAAA CTCACAATGA CATTATGCGT 
CGTCAACTAC GAATTTACGG TGGTTACGAA GTAAAGACAG AAGGAGACGC CTTTATGGTG 
GCATTTCCTA CGCCAACTAG TGGTCTGACA TGGTGCTTAA GTGTTCAATT AAAACTCTTG 
GATGCACAAT GGCCGGAGGA AATTACCTCA GTTCAAGACG GCTGCCAAGT TACGGATAGA 
AATGGTAACA TTATCTATCA AGGCCTATCA GTTAGAATGG GTATTCATTG GGGCTGCCCA 
GTTCCAGAGC TTGATTTAGT GACTCAAAGA ATGGACTATT TGGGGCCGAT GGTCAATAAG 
GCAGCAAGGG TCCAGGGCGT CGCTGACGGT GGTCAGATTG CAATGAGTAG TGATTTTTAC 
TCTGAATTCA ACAAGATAAT GAAGTATCAT GAGCGAGTAG TGAAGGGCAA GGAATCTCTC 
AAGGAAGTTT ATGGTGAAGA AATTATCGGA GAGGTTCTTG AAAGAGAAAT TGCCATGCTG 
GAAAGTATTG GTTGGGCATT TTTTGACTTT GGCGAGCATA AGCTAAAGGG ACTCGAAACC 
AAAGAACTCG TTACTATTGC GTATCCTAAG ATTCTTGCTT CCAGACACGA ATTTGCATCT 
GAAGATGAGC AGTCAAAATT AATCAATGAA ACGATGTTGT TTCGTTTAAG AGTCATTTCA 
AACAGACTGG AATCTATAAT GTCAGCTTTA AGCGGCGGAT TTATTGAACT AGACTCTCGG 
ACGGAGGGAA GTTATATTAA ATTTAACCCT AAAGTTGAAA ATGGTATTAT GCAATCGATT 
TCTGAGAAGG ATGCGTTGTT ATTTTTTGAT CATGTAATTA CTAGAATCGA ATCCAGTGTG 
GCATTATTAC ATTTACGACA ACAGAGGTGT TCAGGACTGG AAATTTGCAG AAACGATAAA 
ACATCTGCTC GAAGCAATAT TTTCAATGTT GTTGACGAAC TTTTACAAAT GGTTAAGAAC 
GCAAAGGATT TATCAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012529.3
CDS	1..6081
Translation	MSSKPDTGSEISGPQRQEEQEQQIEQSSPTEANDRSIHDEVPKVKKRHEQNSGHKSRRNSAYSYYSPRSLSMTKSRESITPNGMDDVSISNVEHPRPTEPKIKRGPYLLKKTLSSLSMTSANSTHDDNKDHGYALNSSKTHNYTSTHNHHDGHHDHHHVQFFPNRKPSLAETLFKRFSGSNSHDGNKSGKESKVANLSLSTVNPAPANRKPSKDSTLSNHLADNVPSTLRRKVSSLVRGSSVHDINNGIADKQIRPKAVAQSENTLHSSDVPNSKRSHRKSFLLGSTSSSSSRRGSNVSSMTNSDSASMATSGSHVLQHNVSNVSPTTKSKDSVNSESADHTNNKSEKVTPEYNENIPENSNSDNKREATTPTIETPISCKPSLFRLDTNLEDVTDITKTVPPTAVNSTLNSTHGTETASPKTVIMPEGPRKSVSMADLSVAAAAPNGEFTSTSNDRSQWVAPQSWDVETKRKKTKPKGRSKSRRSSIDADELDPMSPGPPSKKDSRHHHDRKDNESMVTAGDSNSSFVDICKENVPNDSKTALDTKSVNRLKSNLAMSPPSIRYAPSNLDGDYDTSSTSSSLPSSSISSEDTSSCSDSSSYTNAYMEANREQDNKTPILNKTKSYTKKFTSSSVNMNSPDGAQSSGLLLQDEKDDEVECQLEHYYKDFSDLDPKRHYAIRIFNTDDTFTTLSCTPATTVEEIIPALKRKFNITAQGNFQISLKVGKLSKILRPTSKPILIERKLLLLNGYRKSDPLHIMGIEDLSFVFKFLFHPVTPSHFTPEQEQRIMRSEFVHVDLRNMDLTTPPIIFYQHTSEIESLDVSNNANIFLPLEFIESSIKLLSLRMVNIRASKFPSNITKAYKLVSLELQRNFIRKVPNSIMKLSNLTILNLQCNELESLPAGFVELKNLQLLDLSSNKFMHYPEVINYCTNLLQIDLSYNKIQSLPQSTKYLVKLAKMNLSHNKLNFIGDLSEMTDLRTLNLRYNRISSIKTNASNLQNLFLTDNRISNFEDTLPKLRALEIQENPITSISFKDFYPKNMTSLTLNKAQLSSIPGELLTKLSFLEKLELNQNNLTRLPQEISKLTKLVFLSVARNKLEYIPPELSQLKSLRTLDLHSNNIRDFVDGMENLELTSLNISSNAFGNSSLENSFYHNMSYGSKLSKSLMFFIAADNQFDDAMWPLFNCFVNLKVLNLSYNNFSDVSHMKLESITELYLSGNKLTTLSGDTVLKWSSLKTLMLNSNQMLSLPAELSNLSQLSVFDVGANQLKYNISNYHYDWNWRNNKELKYLNFSGNRRFEIKSFISHDIDADLSDLTVLPQLKVLGLMDVTLNTTKVPDENVNFRLRTTASIINGMRYGVADTLGQRDYVSSRDVTFERFRGNDDECLLCLHDSKNQNADYGHNISRIVRDIYDKILIRQLERYGDETDDNIKTALRFSFLQLNKEINGMLNSVDNGADVANLSYADLLSGACSTVIYIRGKKLFAANLGDCMAILSKNNGDYQTLTKQHLPTKREEYERIRISGGYVNNGKLDGVVDVSRAVGFFDLLPHIHASPDISVVTLTKADEMLIVATHKLWEYMDVDTVCDIARENSTDPLRAAAELKDHAMAYGCTENITILCLALYENIQQQNRFTLNKNSLMTRRSTFEDTTLRRLQPEISPPTGNLAMVFTDIKSSTFLWELFPNAMRTAIKTHNDIMRRQLRIYGGYEVKTEGDAFMVAFPTPTSGLTWCLSVQLKLLDAQWPEEITSVQDGCQVTDRNGNIIYQGLSVRMGIHWGCPVPELDLVTQRMDYLGPMVNKAARVQGVADGGQIAMSSDFYSEFNKIMKYHERVVKGKESLKEVYGEEIIGEVLEREIAMLESIGWAFFDFGEHKLKGLETKELVTIAYPKILASRHEFASEDEQSKLINETMLFRLRVISNRLESIMSALSGGFIELDSRTEGSYIKFNPKVENGIMQSISEKDALLFFDHVITRIESSVALLHLRQQRCSGLEICRNDKTSARSNIFNVVDELLQMVKNAKDLST

Target ORF information:

RefSeq Version	NM_001181439.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C adenylate cyclase (CYR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181439.3

ORF Insert Sequence:

ATGTCATCAA AACCTGATAC TGGTTCGGAA ATTTCTGGCC CTCAGCGACA GGAAGAACAA 
GAACAACAGA TAGAGCAGAG CTCACCTACG GAAGCAAACG ATAGAAGCAT TCATGATGAG 
GTACCAAAAG TCAAGAAGCG TCACGAACAA AATAGTGGTC ACAAATCAAG AAGGAATAGC 
GCATATAGTT ATTACAGCCC ACGGTCGCTT TCTATGACCA AAAGCAGGGA GAGTATCACT 
CCAAATGGTA TGGATGATGT AAGTATTTCG AACGTGGAAC ATCCAAGGCC GACAGAACCG 
AAAATCAAAA GGGGTCCATA TTTACTGAAG AAAACATTGA GCAGTCTTTC AATGACGAGC 
GCGAATAGTA CTCATGATGA TAATAAAGAC CACGGTTACG CTTTGAATTC ATCCAAGACG 
CACAACTACA CATCTACTCA TAACCATCAT GACGGTCATC ATGATCATCA TCATGTTCAG 
TTTTTTCCCA ATAGGAAGCC ATCATTAGCG GAAACCCTAT TCAAAAGGTT TTCAGGGTCA 
AACAGTCACG ATGGCAATAA GTCAGGAAAG GAAAGTAAAG TTGCTAACCT TTCCCTTTCA 
ACGGTAAATC CTGCACCTGC TAATAGGAAA CCTTCTAAAG ACTCCACTTT ATCTAATCAC 
TTGGCTGATA ACGTGCCAAG CACTTTACGA AGGAAAGTGT CCTCATTGGT ACGTGGTTCT 
TCCGTCCATG ATATAAATAA TGGTATTGCA GATAAACAGA TTAGACCAAA GGCTGTTGCG 
CAATCAGAAA ATACATTACA TTCATCCGAT GTTCCCAATA GCAAACGCTC GCACAGAAAA 
AGCTTTCTGC TAGGCTCCAC ATCTTCTTCA AGCAGTAGAA GAGGTTCAAA TGTCAGTTCA 
ATGACTAACA GTGACAGTGC AAGTATGGCG ACGTCGGGTA GTCATGTTCT CCAACATAAC 
GTATCTAATG TTTCTCCAAC TACTAAAAGT AAGGACAGCG TTAACAGCGA ATCCGCCGAT 
CACACTAATA ATAAATCCGA GAAAGTGACT CCAGAATATA ATGAGAACAT TCCGGAAAAT 
TCTAACTCTG ACAACAAACG CGAAGCCACA ACGCCTACTA TAGAAACACC CATTTCATGT 
AAACCATCCC TTTTCAGGCT AGATACAAAC CTTGAGGATG TTACTGATAT TACAAAGACG 
GTGCCACCCA CCGCTGTCAA TTCTACACTA AATTCTACAC ACGGGACTGA GACTGCCTCA 
CCCAAAACGG TGATCATGCC TGAAGGTCCT AGGAAGTCGG TGTCAATGGC TGATCTCTCC 
GTCGCTGCCG CAGCACCTAA TGGTGAATTC ACATCAACTT CCAATGATAG ATCACAATGG 
GTAGCACCTC AAAGCTGGGA TGTGGAAACC AAAAGGAAAA AAACAAAACC TAAAGGGAGA 
TCGAAATCAA GAAGGTCAAG TATAGATGCT GATGAACTTG ATCCCATGTC ACCGGGGCCA 
CCTTCAAAAA AAGACTCTCG TCATCATCAC GATCGAAAGG ATAACGAATC AATGGTCACT 
GCGGGTGACA GTAACTCAAG TTTTGTTGAT ATATGTAAAG AAAACGTTCC GAATGATAGC 
AAGACCGCAC TCGATACTAA ATCTGTGAAC CGCTTAAAAA GTAATTTGGC TATGAGTCCC 
CCAAGTATAC GATATGCTCC ATCAAATTTA GATGGGGACT ACGACACGTC TTCCACTTCC 
TCATCTTTAC CGTCCTCATC TATTAGTTCA GAAGATACAT CTTCCTGCAG CGATTCCTCT 
TCGTACACTA ACGCGTATAT GGAGGCCAAC CGAGAGCAGG ATAATAAAAC ACCGATCCTG 
AATAAAACGA AATCGTATAC CAAGAAATTT ACATCCTCTT CGGTAAATAT GAATTCACCA 
GATGGTGCCC AGAGTTCTGG ATTATTACTA CAAGATGAGA AGGACGATGA GGTCGAGTGC 
CAACTGGAAC ATTACTATAA AGATTTCAGT GATTTAGATC CAAAGAGGCA CTATGCTATT 
CGTATATTCA ATACTGATGA CACTTTTACG ACTCTCTCAT GTACTCCAGC GACTACCGTC 
GAAGAGATAA TACCTGCACT TAAAAGAAAA TTTAACATTA CAGCGCAAGG GAATTTTCAA 
ATTTCCCTGA AGGTGGGAAA GTTGTCAAAA ATTTTGAGAC CAACTTCGAA ACCTATTTTA 
ATTGAAAGAA AACTTTTACT TTTGAATGGT TATCGAAAGT CAGACCCACT TCATATTATG 
GGTATAGAGG ATTTAAGTTT TGTTTTTAAG TTTCTTTTCC ATCCTGTCAC ACCTTCTCAC 
TTTACTCCTG AACAAGAACA AAGAATAATG AGAAGCGAAT TTGTTCACGT AGATTTAAGG 
AATATGGATC TGACTACACC TCCCATCATT TTTTACCAGC ATACGTCAGA AATAGAAAGT 
TTAGACGTTT CTAATAACGC AAATATATTC CTACCTCTGG AGTTCATTGA AAGCTCGATT 
AAATTATTAA GTTTGAGAAT GGTTAATATT AGAGCATCTA AATTTCCTTC CAATATCACT 
AAGGCGTATA AACTAGTATC TTTGGAATTA CAGAGAAACT TCATAAGAAA AGTACCGAAC 
TCAATCATGA AACTGAGTAA TTTAACGATA TTAAACCTTC AATGTAATGA GCTTGAAAGC 
CTACCGGCTG GATTTGTTGA ACTGAAAAAT CTGCAATTGC TAGACTTGTC TTCAAACAAG 
TTCATGCACT ACCCAGAAGT TATTAACTAC TGCACCAATC TTTTACAAAT AGACCTATCA 
TATAATAAAA TCCAAAGCTT ACCACAGTCC ACTAAGTACC TAGTAAAGCT TGCGAAGATG 
AACCTTTCTC ATAACAAACT AAATTTTATA GGCGACTTAT CGGAAATGAC AGATTTGAGG 
ACGCTGAACC TAAGATATAA CAGAATATCA TCAATTAAGA CAAATGCGTC TAACTTGCAG 
AACCTTTTTT TAACAGATAA TAGAATTTCG AACTTTGAAG ACACTTTGCC GAAACTAAGA 
GCCCTTGAAA TTCAAGAGAA TCCAATCACT TCTATATCCT TCAAAGATTT TTATCCAAAA 
AACATGACAA GTTTGACGTT GAACAAGGCA CAGTTATCGA GTATTCCTGG AGAATTACTC 
ACCAAACTAT CTTTCCTCGA GAAACTTGAA CTTAATCAGA ATAATTTGAC TAGACTGCCA 
CAGGAGATAT CCAAGTTGAC TAAATTAGTT TTCCTTTCAG TGGCGAGAAA CAAACTAGAG 
TATATTCCAC CCGAGCTATC TCAACTGAAA AGTTTGAGGA CATTAGATCT ACATTCTAAC 
AACATAAGGG ACTTTGTTGA CGGTATGGAA AACCTTGAAC TAACATCGCT AAATATTTCA 
TCGAATGCAT TCGGTAACTC TAGCTTAGAA AATTCTTTTT ACCATAACAT GTCATATGGG 
TCAAAGTTAT CTAAAAGCCT GATGTTTTTT ATTGCTGCAG ACAATCAATT TGATGATGCT 
ATGTGGCCTC TTTTCAATTG CTTTGTCAAT CTGAAAGTGC TAAATCTTTC TTACAACAAT 
TTTTCAGATG TATCGCACAT GAAACTTGAG AGCATTACCG AATTGTACCT CTCCGGTAAT 
AAGCTCACGA CATTGTCGGG TGATACAGTT TTGAAATGGA GCTCTTTAAA GACTTTAATG 
TTGAATAGTA ACCAAATGTT ATCTCTGCCT GCAGAATTAT CAAATCTCTC ACAGCTAAGT 
GTATTTGATG TTGGAGCAAA TCAATTAAAG TATAATATAT CAAACTATCA TTACGATTGG 
AACTGGAGGA ATAATAAAGA ACTAAAATAT TTGAATTTTT CAGGAAATCG AAGGTTTGAA 
ATAAAGTCAT TTATAAGTCA CGATATTGAT GCTGATTTGT CAGATCTGAC AGTATTACCT 
CAGTTAAAGG TACTAGGTTT AATGGACGTA ACTTTAAATA CTACCAAAGT ACCGGATGAA 
AATGTCAATT TCCGTTTAAG GACAACTGCA TCAATAATAA ATGGGATGCG CTACGGTGTT 
GCTGATACAT TAGGTCAAAG AGACTATGTG TCATCTCGTG ATGTTACCTT TGAAAGATTC 
CGCGGAAATG ACGACGAATG CTTACTATGT CTTCATGATA GTAAAAACCA AAATGCAGAT 
TATGGCCACA ATATATCAAG AATTGTTAGA GATATTTACG ATAAAATACT GATCAGACAA 
CTGGAAAGGT ATGGAGACGA AACAGATGAT AATATAAAAA CTGCACTTCG TTTCAGTTTT 
TTGCAACTGA ATAAGGAGAT TAACGGAATG CTAAATTCTG TTGATAATGG TGCCGATGTT 
GCCAATCTTT CATATGCAGA CTTGCTAAGT GGCGCTTGCT CTACTGTGAT ATATATCAGA 
GGGAAGAAAC TCTTCGCTGC AAATTTAGGT GACTGTATGG CTATTTTATC CAAAAACAAT 
GGTGACTACC AAACGCTAAC CAAACAACAT CTCCCAACAA AGCGGGAAGA ATACGAGAGG 
ATCAGAATAT CTGGCGGGTA TGTCAACAAT GGAAAATTAG ATGGTGTTGT AGATGTGTCT 
AGAGCAGTGG GTTTTTTTGA TTTGCTTCCC CACATTCATG CTTCTCCCGA CATATCTGTC 
GTGACATTAA CAAAAGCAGA CGAGATGCTT ATTGTAGCAA CGCATAAGTT ATGGGAATAC 
ATGGACGTGG ATACAGTTTG TGATATCGCG CGTGAGAATA GTACTGATCC ACTCCGTGCC 
GCAGCTGAGT TGAAGGATCA TGCCATGGCT TACGGCTGTA CAGAGAATAT TACAATTTTG 
TGCCTTGCTC TTTACGAGAA CATTCAGCAA CAAAATCGGT TCACTTTAAA TAAAAACTCT 
TTAATGACTA GAAGAAGTAC TTTCGAGGAT ACTACATTAA GAAGACTTCA ACCTGAGATT 
TCTCCGCCAA CAGGTAACCT AGCAATGGTC TTCACTGATA TCAAAAGCTC AACCTTCTTA 
TGGGAGCTAT TCCCTAACGC AATGAGGACC GCAATAAAAA CTCACAATGA CATTATGCGT 
CGTCAACTAC GAATTTACGG TGGTTACGAA GTAAAGACAG AAGGAGACGC CTTTATGGTG 
GCATTTCCTA CGCCAACTAG TGGTCTGACA TGGTGCTTAA GTGTTCAATT AAAACTCTTG 
GATGCACAAT GGCCGGAGGA AATTACCTCA GTTCAAGACG GCTGCCAAGT TACGGATAGA 
AATGGTAACA TTATCTATCA AGGCCTATCA GTTAGAATGG GTATTCATTG GGGCTGCCCA 
GTTCCAGAGC TTGATTTAGT GACTCAAAGA ATGGACTATT TGGGGCCGAT GGTCAATAAG 
GCAGCAAGGG TCCAGGGCGT CGCTGACGGT GGTCAGATTG CAATGAGTAG TGATTTTTAC 
TCTGAATTCA ACAAGATAAT GAAGTATCAT GAGCGAGTAG TGAAGGGCAA GGAATCTCTC 
AAGGAAGTTT ATGGTGAAGA AATTATCGGA GAGGTTCTTG AAAGAGAAAT TGCCATGCTG 
GAAAGTATTG GTTGGGCATT TTTTGACTTT GGCGAGCATA AGCTAAAGGG ACTCGAAACC 
AAAGAACTCG TTACTATTGC GTATCCTAAG ATTCTTGCTT CCAGACACGA ATTTGCATCT 
GAAGATGAGC AGTCAAAATT AATCAATGAA ACGATGTTGT TTCGTTTAAG AGTCATTTCA 
AACAGACTGG AATCTATAAT GTCAGCTTTA AGCGGCGGAT TTATTGAACT AGACTCTCGG 
ACGGAGGGAA GTTATATTAA ATTTAACCCT AAAGTTGAAA ATGGTATTAT GCAATCGATT 
TCTGAGAAGG ATGCGTTGTT ATTTTTTGAT CATGTAATTA CTAGAATCGA ATCCAGTGTG 
GCATTATTAC ATTTACGACA ACAGAGGTGT TCAGGACTGG AAATTTGCAG AAACGATAAA 
ACATCTGCTC GAAGCAATAT TTTCAATGTT GTTGACGAAC TTTTACAAAT GGTTAAGAAC 
GCAAAGGATT TATCAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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CYR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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