ORF » Species Summary » Saccharomyces cerevisiae S288C » HFM1 cDNA ORF clone

HFM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following HFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02029 NM_001181117.1
Latest version!		 Saccharomyces cerevisiae S288C DNA helicase (HFM1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    HFM1 ( NM_001181117.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011263.2 Saccharomyces cerevisiae S288C DNA helicase (HFM1), partial mRNA.
    Price & Availability↑
    CloneID OSi02029
    Clone ID Related Accession (Same CDS sequence) NM_001181117.1
    Accession Version NM_001181117.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product DNA helicase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAACAA AGTTTGATCG CCTCGGTACA GGAAAAAGAA GTAGACCCTC TCCAAATAAT 
    ATTGACTTTA ACGACCAGTC TGCTACATTT AAAAGAAATA AGAAAAATAG CCGCCAACCA 
    AGTTTTAAAG TAGGTTTGTC TTATAACAGT TTACTGGATG ATTGTGATGA TGAAAATGAA 
    ACAGAGGAAA TATTTGAGGG CCGTGGTCTG CAATTCTTCG ACAAAGACGA TAATTTTTCA 
    ATAACAGCAG ATGATACACA AGTAACTAGT AAATTATTTG ATCACGATTT AGAGCAGACA 
    CCTGATGAAG AGGCGAAGAA ACCTAAGAAG GTCACGATAA GGAAATCAGC GAAGAAATGT 
    TTGAGCACGA CCATATTACC AGATTCATTC AGAGGGGTTT TTAAATTTAC AGAATTTAAT 
    AAAATGCAAT CAGAGGCTTT TCCTAGTATT TATGAGAGTA ACGAGAACTG CATAATTTCT 
    TCACCAACCG GATCAGGCAA GACTGTATTA TTTGAATTGG CAATATTACG TCTTATAAAG 
    GAAACAAATA GTGATACCAA TAACACCAAA ATTATATACA TCGCGCCAAC CAAATCTCTA 
    TGTTACGAGA TGTACAAGAA CTGGTTTCCT TCCTTTGTGA ATCTTTCTGT TGGTATGCTT 
    ACTAGTGATA CCTCTTTTCT GGAAACTGAA AAGGCTAAAA AATGTAACAT AATAATTACG 
    ACACCAGAAA AATGGGATTT GTTAACAAGA AGATGGTCCG ATTACAGCCG ATTATTCGAA 
    TTGGTCAAAC TTGTCCTAGT TGATGAGATT CATACCATAA AGGAGAAAAG AGGAGCATCT 
    TTGGAAGTAA TTCTGACAAG GATGAATACA ATGTGTCAAA ACATTCGGTT TGTTGCTTTA 
    AGTGCAACAG TTCCAAATAT AGAAGACCTA GCATTGTGGC TCAAAACTAA CAACGAGCTT 
    CCTGCAAATA TTCTTTCTTT TGACGAATCG TACAGGCAAG TTCAGTTAAC GAAGTTTGTT 
    TATGGGTACT CCTTCAATTG CAAAAATGAC TTCCAAAAAG ATGCTATATA TAATTCCAAA 
    TTGATTGAAA TAATTGAAAA GCATGCCGAT AATCGTCCCG TACTAATATT TTGTCCGACT 
    AGGGCTTCAA CTATATCAAC AGCAAAATTT TTATTAAATA ATCATATTTT TTCAAAAAGT 
    AAGAAAAGAT GTAATCATAA TCCCTCCGAT AAAATATTAA ATGAATGTAT GCAACAAGGT 
    ATCGCTTTCC ATCATGCTGG AATTTCTTTA GAAGACCGTA CTGCCGTTGA GAAAGAATTT 
    CTTGCAGGTT CAATTAATAT ATTGTGTTCA ACTTCAACGT TAGCTGTTGG TGTGAACCTT 
    CCCGCATATT TGGTCATTAT CAAAGGCACC AAAAGTTGGA ACTCTTCTGA AATCCAAGAG 
    TATTCAGACC TAGATGTACT GCAAATGATT GGGCGGGCAG GAAGACCTCA ATTTGAAACT 
    CACGGGTGCG CAGTAATCAT GACAGACTCT AAAATGAAGC AAACATATGA AAACTTAATT 
    CATGGAACTG ACGTTTTGGA AAGTTCATTA CATTTGAATT TGATCGAGCA TTTAGCAGCA 
    GAAACCTCTT TGGAAACTGT ATATTCAATT GAAACTGCAG TAAATTGGCT ACGAAACACA 
    TTCTTTTACG TTAGATTTGG GAAGAATCCA GCTGCATATC AAGAGGTGAA CAGATACGTT 
    AGTTTTCATT CCGTTGAAGA CTCTCAGATA AATCAATTCT GTCAGTATTT GCTTGACACC 
    TTAGTAAAAG TGAAAATAAT CGATATCAGC AATGGGGAGT ACAAATCTAC AGCGTACGGA 
    AATGCAATGA CGCGACATTA TATATCCTTT GAGTCGATGA AACAATTCAT TAACGCAAAA 
    AAATTTCTAT CATTACAAGG GATACTAAAT TTACTGGCCA CTTCTGAAGA GTTTTCAGTT 
    ATGAGGGTGA GGCACAATGA AAAAAAATTG TTCAAAGAAA TCAATTTATC GCCACTTTTG 
    AAATATCCAT TTTTAACCGA GAAGAAACAG AGCCAAATAA TTGACAGGGT TAGTCAAAAG 
    GTTTCTCTTT TGATTCAATA TGAGTTAGGT GGGTTAGAAT TTCCATCATA CGAAGGCGCT 
    TCTAAACTAC ATCAAACTCT TGTTCAGGAT AAATTCCTCG TTTTTAGACA TTGTTTTAGA 
    TTACTAAAAT GCATGGTAGA CACATTTATT GAAAAAAGTG ATGGAACATC ACTGAAAAAT 
    ACTTTGTTCT TACTGCGAAG CCTGAATGGT CACTGCTGGG AAAACACGCC AATGGTTCTT 
    AGGCAATTGA AAACAATTGG ACTAGTTTCA GTAAGAAGGC TAATACGACA TGGAATTACC 
    AATCTAGAAG AAATGGGGCA TTTGTCAGAT ACTCAGATTG AATACTATCT TAACCTCAAG 
    ATTGGAAACG GCATAAAAAT TAAAAACGAC ATTTCTTTGT TGCCTTGTCT GAATATTAGA 
    ACAAAACTAG AAAATTGCAA AATAGAAAAT GAAGAGTTGT GGTTGACATT TAAAGTTGAG 
    ATTAGTGCAA CATTCAAGTC TAGCATTTGG CATGGTCAGC ACCTCTCATT AGATATCGAA 
    ACCGAGAAAA GCTCAGGAGA GTTAATCGAC TTTAGAAGAT TGCAAGTTAA CAAACTACAG 
    TCACCAAGGG GCTTTAGAAT TTCAGCAAAA ATATCACCAA AACTAGAAAA AATAGAATTC 
    TCAATTCATT GCCAAGAAAT CGCCGGACTC GGCAAAACAA TCGTATATTC CACTGATCAT 
    TTGGCATCAC AGTTCTCTGC GAAGACACCA AATATAAGGA AAGATCTAAA TAGTTTAGAA 
    AAATGCCTCT TCTATGAAAG CAGTAGTGAC GGAGAAGTGG GGAAAACGTC TAGGGTTTCC 
    CATAAAGATG GATTAGAAGA ATCGCTATCC TCAGATGATA GTATACTAGA TTATTTGAAT 
    GAAAGGAAGA AATCATCAAA GGCGGTTGAA TCTGCAGCAG TTATTCACCC GGAGGCACAC 
    TCTTCTTCAC ATTTTAGTAA TGGCCGCCAA GTAAGATCTA ATGGCAATTA CGAGTGTTTT 
    CATAGCTGCA AAGACAAAAC GCAATGCAGA CATTTATGTT GTAAAGAGGG TATTCCAGTA 
    AAGTATATAA AAGAAAAAGG ACCCTCCTCA ATCAAGCCAG TATCAAAACC TGATCAAATA 
    CGCCAGCCAC TGCTTGCAAA AAATATCAAC ACAACCCCTC ACCTGGAAAA AAGACTGAAT 
    AGTAAACCAA AGCAGTGGCA AGAAGAAAAT ACAGATATTG CGACAGTTCA TACACTTCCT 
    TCAAAAATTT ATAATCTATC GCAACAGATG TCGTCTATGG AAGCGGGTGA ACAAGTCCTA 
    AAATCAGGCC CCGAAAACTG CCCTGAAATA ATTCCTATTG ATCTTGAATC ATCCGATAGT 
    TATTCTAGTA ATACAGCGGC GAGCTCAATA AGTGATCCGA ATGGTGATCT GGATTTCTTG 
    GGTTCCGATA TAGAGTTTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011263.2
    CDS1..3564
    Translation

    Target ORF information:

    RefSeq Version NM_001181117.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C DNA helicase (HFM1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181117.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAAACAA AGTTTGATCG CCTCGGTACA GGAAAAAGAA GTAGACCCTC TCCAAATAAT 
    ATTGACTTTA ACGACCAGTC TGCTACATTT AAAAGAAATA AGAAAAATAG CCGCCAACCA 
    AGTTTTAAAG TAGGTTTGTC TTATAACAGT TTACTGGATG ATTGTGATGA TGAAAATGAA 
    ACAGAGGAAA TATTTGAGGG CCGTGGTCTG CAATTCTTCG ACAAAGACGA TAATTTTTCA 
    ATAACAGCAG ATGATACACA AGTAACTAGT AAATTATTTG ATCACGATTT AGAGCAGACA 
    CCTGATGAAG AGGCGAAGAA ACCTAAGAAG GTCACGATAA GGAAATCAGC GAAGAAATGT 
    TTGAGCACGA CCATATTACC AGATTCATTC AGAGGGGTTT TTAAATTTAC AGAATTTAAT 
    AAAATGCAAT CAGAGGCTTT TCCTAGTATT TATGAGAGTA ACGAGAACTG CATAATTTCT 
    TCACCAACCG GATCAGGCAA GACTGTATTA TTTGAATTGG CAATATTACG TCTTATAAAG 
    GAAACAAATA GTGATACCAA TAACACCAAA ATTATATACA TCGCGCCAAC CAAATCTCTA 
    TGTTACGAGA TGTACAAGAA CTGGTTTCCT TCCTTTGTGA ATCTTTCTGT TGGTATGCTT 
    ACTAGTGATA CCTCTTTTCT GGAAACTGAA AAGGCTAAAA AATGTAACAT AATAATTACG 
    ACACCAGAAA AATGGGATTT GTTAACAAGA AGATGGTCCG ATTACAGCCG ATTATTCGAA 
    TTGGTCAAAC TTGTCCTAGT TGATGAGATT CATACCATAA AGGAGAAAAG AGGAGCATCT 
    TTGGAAGTAA TTCTGACAAG GATGAATACA ATGTGTCAAA ACATTCGGTT TGTTGCTTTA 
    AGTGCAACAG TTCCAAATAT AGAAGACCTA GCATTGTGGC TCAAAACTAA CAACGAGCTT 
    CCTGCAAATA TTCTTTCTTT TGACGAATCG TACAGGCAAG TTCAGTTAAC GAAGTTTGTT 
    TATGGGTACT CCTTCAATTG CAAAAATGAC TTCCAAAAAG ATGCTATATA TAATTCCAAA 
    TTGATTGAAA TAATTGAAAA GCATGCCGAT AATCGTCCCG TACTAATATT TTGTCCGACT 
    AGGGCTTCAA CTATATCAAC AGCAAAATTT TTATTAAATA ATCATATTTT TTCAAAAAGT 
    AAGAAAAGAT GTAATCATAA TCCCTCCGAT AAAATATTAA ATGAATGTAT GCAACAAGGT 
    ATCGCTTTCC ATCATGCTGG AATTTCTTTA GAAGACCGTA CTGCCGTTGA GAAAGAATTT 
    CTTGCAGGTT CAATTAATAT ATTGTGTTCA ACTTCAACGT TAGCTGTTGG TGTGAACCTT 
    CCCGCATATT TGGTCATTAT CAAAGGCACC AAAAGTTGGA ACTCTTCTGA AATCCAAGAG 
    TATTCAGACC TAGATGTACT GCAAATGATT GGGCGGGCAG GAAGACCTCA ATTTGAAACT 
    CACGGGTGCG CAGTAATCAT GACAGACTCT AAAATGAAGC AAACATATGA AAACTTAATT 
    CATGGAACTG ACGTTTTGGA AAGTTCATTA CATTTGAATT TGATCGAGCA TTTAGCAGCA 
    GAAACCTCTT TGGAAACTGT ATATTCAATT GAAACTGCAG TAAATTGGCT ACGAAACACA 
    TTCTTTTACG TTAGATTTGG GAAGAATCCA GCTGCATATC AAGAGGTGAA CAGATACGTT 
    AGTTTTCATT CCGTTGAAGA CTCTCAGATA AATCAATTCT GTCAGTATTT GCTTGACACC 
    TTAGTAAAAG TGAAAATAAT CGATATCAGC AATGGGGAGT ACAAATCTAC AGCGTACGGA 
    AATGCAATGA CGCGACATTA TATATCCTTT GAGTCGATGA AACAATTCAT TAACGCAAAA 
    AAATTTCTAT CATTACAAGG GATACTAAAT TTACTGGCCA CTTCTGAAGA GTTTTCAGTT 
    ATGAGGGTGA GGCACAATGA AAAAAAATTG TTCAAAGAAA TCAATTTATC GCCACTTTTG 
    AAATATCCAT TTTTAACCGA GAAGAAACAG AGCCAAATAA TTGACAGGGT TAGTCAAAAG 
    GTTTCTCTTT TGATTCAATA TGAGTTAGGT GGGTTAGAAT TTCCATCATA CGAAGGCGCT 
    TCTAAACTAC ATCAAACTCT TGTTCAGGAT AAATTCCTCG TTTTTAGACA TTGTTTTAGA 
    TTACTAAAAT GCATGGTAGA CACATTTATT GAAAAAAGTG ATGGAACATC ACTGAAAAAT 
    ACTTTGTTCT TACTGCGAAG CCTGAATGGT CACTGCTGGG AAAACACGCC AATGGTTCTT 
    AGGCAATTGA AAACAATTGG ACTAGTTTCA GTAAGAAGGC TAATACGACA TGGAATTACC 
    AATCTAGAAG AAATGGGGCA TTTGTCAGAT ACTCAGATTG AATACTATCT TAACCTCAAG 
    ATTGGAAACG GCATAAAAAT TAAAAACGAC ATTTCTTTGT TGCCTTGTCT GAATATTAGA 
    ACAAAACTAG AAAATTGCAA AATAGAAAAT GAAGAGTTGT GGTTGACATT TAAAGTTGAG 
    ATTAGTGCAA CATTCAAGTC TAGCATTTGG CATGGTCAGC ACCTCTCATT AGATATCGAA 
    ACCGAGAAAA GCTCAGGAGA GTTAATCGAC TTTAGAAGAT TGCAAGTTAA CAAACTACAG 
    TCACCAAGGG GCTTTAGAAT TTCAGCAAAA ATATCACCAA AACTAGAAAA AATAGAATTC 
    TCAATTCATT GCCAAGAAAT CGCCGGACTC GGCAAAACAA TCGTATATTC CACTGATCAT 
    TTGGCATCAC AGTTCTCTGC GAAGACACCA AATATAAGGA AAGATCTAAA TAGTTTAGAA 
    AAATGCCTCT TCTATGAAAG CAGTAGTGAC GGAGAAGTGG GGAAAACGTC TAGGGTTTCC 
    CATAAAGATG GATTAGAAGA ATCGCTATCC TCAGATGATA GTATACTAGA TTATTTGAAT 
    GAAAGGAAGA AATCATCAAA GGCGGTTGAA TCTGCAGCAG TTATTCACCC GGAGGCACAC 
    TCTTCTTCAC ATTTTAGTAA TGGCCGCCAA GTAAGATCTA ATGGCAATTA CGAGTGTTTT 
    CATAGCTGCA AAGACAAAAC GCAATGCAGA CATTTATGTT GTAAAGAGGG TATTCCAGTA 
    AAGTATATAA AAGAAAAAGG ACCCTCCTCA ATCAAGCCAG TATCAAAACC TGATCAAATA 
    CGCCAGCCAC TGCTTGCAAA AAATATCAAC ACAACCCCTC ACCTGGAAAA AAGACTGAAT 
    AGTAAACCAA AGCAGTGGCA AGAAGAAAAT ACAGATATTG CGACAGTTCA TACACTTCCT 
    TCAAAAATTT ATAATCTATC GCAACAGATG TCGTCTATGG AAGCGGGTGA ACAAGTCCTA 
    AAATCAGGCC CCGAAAACTG CCCTGAAATA ATTCCTATTG ATCTTGAATC ATCCGATAGT 
    TATTCTAGTA ATACAGCGGC GAGCTCAATA AGTGATCCGA ATGGTGATCT GGATTTCTTG 
    GGTTCCGATA TAGAGTTTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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