ORF » Species Summary » Saccharomyces cerevisiae S288C » MIS1 cDNA ORF clone

MIS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following MIS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01791 NM_001178432.1
Latest version!		 Saccharomyces cerevisiae S288C trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1 (MIS1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MIS1 ( NM_001178432.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009640.1 Saccharomyces cerevisiae S288C trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1 (MIS1), partial mRNA.
    Price & Availability↑
    CloneID OSi01791
    Clone ID Related Accession (Same CDS sequence) NM_001178432.1
    Accession Version NM_001178432.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2928bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTTGTCGA GACTATCTTT ATTGAGTAAC TCGAGGGCGT TCCAACAGGC CAGATGGCGC 
    ATTTACCGCT TAAAAGTTTC GCCAACTGTG CATGCTTCTC AATACCATAT TCTTTCTGGT 
    AGGAAACTTG CTCAATCTAT TCGAGAAAAA GCCAATGATG AGATACAAGC CATTAAGCTT 
    AAGCATCCTA ATTTCAAGCC TACCTTGAAA ATCATTCAAG TCGGGGCCAG ACCAGACTCA 
    TCTACGTACG TGAGGATGAA ATTGAAAGCT TCAAAGGACA GCAATGTAGA CTGTATCATA 
    GAGAAGTTAC CAGCAGAAAT CACTGAAGTT GAGCTTTTGA AGAAAATTAG TGACATCAAT 
    GATGATGACT CTATCCATGG GTTGCTAATT CAACTACCTC TACCGCGTCA CTTGGATGAA 
    ACCACGATTA CAAACGCTGT GGACTTTAAA AAGGACGTTG ATGGGTTCCA CAGATATAAT 
    GCTGGTGAAT TGGCTAAAAA AGGAGGGAAA CCATACTTCA TACCATGTAC TCCTTATGGT 
    TGCATGAAAT TACTTGAAGA AGCTCATGTT AAGTTAGATG GTAAGAACGC CGTCGTGTTA 
    GGCAGATCAA GTATCGTCGG AAATCCAATT GCTTCGTTGT TGAAAAATGC GAATGCCACT 
    GTTACTGTCT GTCATAGTCA TACAAGGAAC ATTGCAGAAG TGGTCTCCCA AGCTGATATA 
    GTTATCGCAG CTTGCGGTAT TCCTCAATAC GTTAAATCAG ACTGGATTAA AGAAGGCGCC 
    GTGGTTATTG ATGTAGGTAT CAACTACGTA CCTGATATCA GTAAGAAAAG TGGGCAAAAA 
    TTAGTTGGTG ATGTTGATTT TGATTCTGTA AAGGAAAAGA CATCTTATAT TACCCCTGTT 
    CCTGGTGGAG TGGGTCCAAT GACTGTCGCT ATGCTTGTTT CCAATGTACT ATTAGCTGCT 
    AAAAGGCAAT TCGTGGAATC TGAAAAGCTT CCAGTTATCA AACCTCTTCC ATTACACTTA 
    GAAAGTCCAG TGCCTTCAGA TATTGATATA TCAAGAGCTC AGAGTCCTAA GCATATCAAG 
    CAAGTTGCCG AGGAGTTGGG AATCCACTCT CACGAATTAG AATTATACGG CCACTATAAG 
    GCAAAAATTT CTCCAAATAT TTTTAAAAGA TTAGAATCTA GAGAAAACGG TAAGTACGTC 
    CTTGTTGCAG GTATTACTCC GACTCCATTG GGTGAAGGTA AATCCACTAC GACTATGGGG 
    TTGGTGCAGG CTTTATCCGC TCATTTAGGG AAACCATCCA TCGCGAACGT TAGACAACCA 
    TCTCTTGGCC CAACCCTGGG TGTCAAAGGT GGTGCTGCTG GTGGTGGTTA TGCCCAAGTT 
    ATTCCTATGG ACGAGTTCAA TTTACATTTG ACCGGGGATA TTCATGCTAT CAGCGCTGCG 
    AACAATCTTC TTGCAGCAGC TATCGACACT AGAATGTTCC ATGAAGCCAC TCAGAAGAAT 
    GATAGTACAT TTTACAAGAG ACTAGTTCCA AGAAAAAAAG GCATCAGAAA GTTTACCCCA 
    TCCATGCAGA GAAGGCTTAA AAGATTGGAT ATTGAAAAAG AAGACCCTGA TGCTTTAACA 
    CCTGAAGAAG TCAAAAGATT TGCTAGATTG AACATAAATC CCGATACTAT CACTATCAGA 
    AGAGTTGTCG ACATCAATGA CAGGATGTTA AGACAAATTA CCATTGGCGA AGCCGCTACG 
    GAGAAGGGTT TTACAAGGAC CACTGGATTC GATATCACTG TTGCCTCTGA ATTAATGGCC 
    ATTTTAGCTC TATCTAAAAG CTTACACGAG ATGAAGGAAC GTATTGGACG CATGGTTATT 
    GGTGCTGATT ATGATAACAA ACCAGTAACA GTAGAAGATA TTGGCTGTAC CGGTGCTCTG 
    ACTGCATTAT TACGTGACGC TATAAAGCCT AACTTAATGC AAACTTTGGA AGGGACCCCC 
    GTAATGGTTC ACGCTGGTCC TTTCGCCAAC ATCTCCATCG GCGCATCATC AGTAATTGCA 
    GACTTAATGG CATTGAAGCT TGTTGGTTCA GAAAAGAACC CGTTAAATGA CAAGAACATC 
    CATGAACCTG GTTATGTAGT TACTGAAGCA GGATTCGATT TTGCCATGGG TGGTGAAAGA 
    TTCTTTGATA TCAAATGTCG TTCCTCTGGA TTGGTGCCAG ATGCAGTTGT CTTAGTCGCA 
    ACCGTAAGAG CTTTGAAATC TCATGGAGGT GCTCCAAATG TTAAGCCCGG ACAATCATTA 
    CCAAAAGAAT ACACAGAGGA AAACATCGAT TTTGTTGCCA AGGGTGTTAG TAATTTGGTT 
    AAGCAGATTG AAAACATCAA AACGTTTGGA ATACCAGTCG TTGTAGCAAT CAACAGATTT 
    GAAACAGACT CACAGGCAGA GATTGAGGTA ATCAAGAAAG CAGCCTTGAA TGCTGGCGCA 
    TCTCATGCCG TTACTTCTAA TCACTGGATG GAAGGTGGTA AAGGTGCAGT AGAATTAGCA 
    CATGCTGTGG TAGATGCAAC GAAAGAACCA AAGAACTTTA ACTTTTTGTA CGACGTCAAT 
    AGCTCCATCG AGGACAAGCT TACCAGCATC GTCCAAAAAA TGTATGGTGG GGCAAAAATC 
    GAAGTATCAC CAGAAGCCCA AAAAAAGATA GACACCTACA AAAAACAAGG CTTCGGTAAT 
    CTTCCCATCT GTATTGCTAA GACACAATAT TCATTATCCC ATGATCCATC ATTAAAGGGT 
    GTTCCTAGAG GTTTTACGTT CCCCATCAGG GATGTGAGAG CTTCAATAGG TGCAGGTTAT 
    TTATACGCTT TGGCTGCAGA AATTCAAACC ATACCGGGTC TATCGACATA TGCTGGTTAC 
    ATGGCAGTAG AAGTCGACGA CGACGGTGAA ATTGAAGGTC TATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009640.1
    CDS1..2928
    Translation

    Target ORF information:

    RefSeq Version NM_001178432.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1 (MIS1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTTGTCGA GACTATCTTT ATTGAGTAAC TCGAGGGCGT TCCAACAGGC CAGATGGCGC 
    ATTTACCGCT TAAAAGTTTC GCCAACTGTG CATGCTTCTC AATACCATAT TCTTTCTGGT 
    AGGAAACTTG CTCAATCTAT TCGAGAAAAA GCCAATGATG AGATACAAGC CATTAAGCTT 
    AAGCATCCTA ATTTCAAGCC TACCTTGAAA ATCATTCAAG TCGGGGCCAG ACCAGACTCA 
    TCTACGTACG TGAGGATGAA ATTGAAAGCT TCAAAGGACA GCAATGTAGA CTGTATCATA 
    GAGAAGTTAC CAGCAGAAAT CACTGAAGTT GAGCTTTTGA AGAAAATTAG TGACATCAAT 
    GATGATGACT CTATCCATGG GTTGCTAATT CAACTACCTC TACCGCGTCA CTTGGATGAA 
    ACCACGATTA CAAACGCTGT GGACTTTAAA AAGGACGTTG ATGGGTTCCA CAGATATAAT 
    GCTGGTGAAT TGGCTAAAAA AGGAGGGAAA CCATACTTCA TACCATGTAC TCCTTATGGT 
    TGCATGAAAT TACTTGAAGA AGCTCATGTT AAGTTAGATG GTAAGAACGC CGTCGTGTTA 
    GGCAGATCAA GTATCGTCGG AAATCCAATT GCTTCGTTGT TGAAAAATGC GAATGCCACT 
    GTTACTGTCT GTCATAGTCA TACAAGGAAC ATTGCAGAAG TGGTCTCCCA AGCTGATATA 
    GTTATCGCAG CTTGCGGTAT TCCTCAATAC GTTAAATCAG ACTGGATTAA AGAAGGCGCC 
    GTGGTTATTG ATGTAGGTAT CAACTACGTA CCTGATATCA GTAAGAAAAG TGGGCAAAAA 
    TTAGTTGGTG ATGTTGATTT TGATTCTGTA AAGGAAAAGA CATCTTATAT TACCCCTGTT 
    CCTGGTGGAG TGGGTCCAAT GACTGTCGCT ATGCTTGTTT CCAATGTACT ATTAGCTGCT 
    AAAAGGCAAT TCGTGGAATC TGAAAAGCTT CCAGTTATCA AACCTCTTCC ATTACACTTA 
    GAAAGTCCAG TGCCTTCAGA TATTGATATA TCAAGAGCTC AGAGTCCTAA GCATATCAAG 
    CAAGTTGCCG AGGAGTTGGG AATCCACTCT CACGAATTAG AATTATACGG CCACTATAAG 
    GCAAAAATTT CTCCAAATAT TTTTAAAAGA TTAGAATCTA GAGAAAACGG TAAGTACGTC 
    CTTGTTGCAG GTATTACTCC GACTCCATTG GGTGAAGGTA AATCCACTAC GACTATGGGG 
    TTGGTGCAGG CTTTATCCGC TCATTTAGGG AAACCATCCA TCGCGAACGT TAGACAACCA 
    TCTCTTGGCC CAACCCTGGG TGTCAAAGGT GGTGCTGCTG GTGGTGGTTA TGCCCAAGTT 
    ATTCCTATGG ACGAGTTCAA TTTACATTTG ACCGGGGATA TTCATGCTAT CAGCGCTGCG 
    AACAATCTTC TTGCAGCAGC TATCGACACT AGAATGTTCC ATGAAGCCAC TCAGAAGAAT 
    GATAGTACAT TTTACAAGAG ACTAGTTCCA AGAAAAAAAG GCATCAGAAA GTTTACCCCA 
    TCCATGCAGA GAAGGCTTAA AAGATTGGAT ATTGAAAAAG AAGACCCTGA TGCTTTAACA 
    CCTGAAGAAG TCAAAAGATT TGCTAGATTG AACATAAATC CCGATACTAT CACTATCAGA 
    AGAGTTGTCG ACATCAATGA CAGGATGTTA AGACAAATTA CCATTGGCGA AGCCGCTACG 
    GAGAAGGGTT TTACAAGGAC CACTGGATTC GATATCACTG TTGCCTCTGA ATTAATGGCC 
    ATTTTAGCTC TATCTAAAAG CTTACACGAG ATGAAGGAAC GTATTGGACG CATGGTTATT 
    GGTGCTGATT ATGATAACAA ACCAGTAACA GTAGAAGATA TTGGCTGTAC CGGTGCTCTG 
    ACTGCATTAT TACGTGACGC TATAAAGCCT AACTTAATGC AAACTTTGGA AGGGACCCCC 
    GTAATGGTTC ACGCTGGTCC TTTCGCCAAC ATCTCCATCG GCGCATCATC AGTAATTGCA 
    GACTTAATGG CATTGAAGCT TGTTGGTTCA GAAAAGAACC CGTTAAATGA CAAGAACATC 
    CATGAACCTG GTTATGTAGT TACTGAAGCA GGATTCGATT TTGCCATGGG TGGTGAAAGA 
    TTCTTTGATA TCAAATGTCG TTCCTCTGGA TTGGTGCCAG ATGCAGTTGT CTTAGTCGCA 
    ACCGTAAGAG CTTTGAAATC TCATGGAGGT GCTCCAAATG TTAAGCCCGG ACAATCATTA 
    CCAAAAGAAT ACACAGAGGA AAACATCGAT TTTGTTGCCA AGGGTGTTAG TAATTTGGTT 
    AAGCAGATTG AAAACATCAA AACGTTTGGA ATACCAGTCG TTGTAGCAAT CAACAGATTT 
    GAAACAGACT CACAGGCAGA GATTGAGGTA ATCAAGAAAG CAGCCTTGAA TGCTGGCGCA 
    TCTCATGCCG TTACTTCTAA TCACTGGATG GAAGGTGGTA AAGGTGCAGT AGAATTAGCA 
    CATGCTGTGG TAGATGCAAC GAAAGAACCA AAGAACTTTA ACTTTTTGTA CGACGTCAAT 
    AGCTCCATCG AGGACAAGCT TACCAGCATC GTCCAAAAAA TGTATGGTGG GGCAAAAATC 
    GAAGTATCAC CAGAAGCCCA AAAAAAGATA GACACCTACA AAAAACAAGG CTTCGGTAAT 
    CTTCCCATCT GTATTGCTAA GACACAATAT TCATTATCCC ATGATCCATC ATTAAAGGGT 
    GTTCCTAGAG GTTTTACGTT CCCCATCAGG GATGTGAGAG CTTCAATAGG TGCAGGTTAT 
    TTATACGCTT TGGCTGCAGA AATTCAAACC ATACCGGGTC TATCGACATA TGCTGGTTAC 
    ATGGCAGTAG AAGTCGACGA CGACGGTGAA ATTGAAGGTC TATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome II.
    The EMBO journal13(24)5795-809(1994 Dec)
    Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K


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