STU2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STU2
Entrez Gene ID	850734
Full Name	Stu2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181932.1	NP_013146.1	Stu2p

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	alp14	NP_588048.1
Kluyveromyces lactis	KLLA0E16325g	XP_454685.1
Saccharomyces cerevisiae (baker's yeast)	STU2	NP_013146.1
Eremothecium gossypii	AGOS_ACL132C	NP_983272.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TORC1/Kog1 signaling controls Stu2 localization and thereby contributes to proper microtubule cytoskeletal organization in interphase and mitosis.
Title: TORC1 signaling exerts spatial control over microtubule dynamics by promoting nuclear export of Stu2.

findings reveal an uncharacterized function of Stu2 that is regulated mechanically, such that kinetochore-microtubule attachments are intrinsically stabilized by tension, implicating it in the correction of erroneous kinetochore-microtubule attachments
Title: A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments.

Stu2 is a Saccharomyces cerevisiae microtubule polymerase.
Title: Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency.

anaphase Stu2-dependent microtubule dynamics are critical for separation of long chromosomes.
Title: Chromosome length and perinuclear attachment constrain resolution of DNA intertwines.

Stu2p/XMAP215 proteins are essential microtubule polymerases that use multiple alpha-beta-tubulin-interacting TOG domains to bind microtubule plus ends and catalyze fast microtubule growth.
Title: A tethered delivery mechanism explains the catalytic action of a microtubule polymerase.

The following STU2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STU2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00397	NM_001181932.1 Latest version!	Saccharomyces cerevisiae S288C Stu2p (STU2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STU2 ( NM_001181932.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013146.1 Saccharomyces cerevisiae S288C Stu2p (STU2), partial mRNA.

Price & Availability↑

CloneID	OSi00397
Clone ID Related Accession (Same CDS sequence)	NM_001181932.1
Accession Version	NM_001181932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Stu2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGGAG AAGAAGAAGT AGATTATACA ACGTTACCCC TAGAAGAGCG ATTAACGTAC 
AAGTTATGGA AGGCACGTTT AGAAGCTTAT AAGGAGTTAA ACCAGCTTTT TAGAAACTCC 
GTTGGCGACA TCAGTAGGGA TGACAACATA CAAATTTACT GGAGGGATCC TACATTATTT 
GCACAATATA TAACTGATTC TAATGTGGTC GCTCAAGAAC AAGCCATTGT TGCCTTGAAT 
AGTTTAATAG ATGCCTTTGC GTCATCGTCT TTGAAAAATG CACATAATAT TACTTTAATA 
TCAACATGGA CACCATTGCT TGTGGAAAAG GGCTTAACGT CCTCAAGGGC TACAACGAAG 
ACGCAGTCGA TGAGCTGTAT ACTCTCACTA TGTGGTCTTG ATACATCTAT TACGCAATCG 
GTGGAATTGG TAATACCCTT TTTCGAGAAA AAACTGCCAA AATTAATTGC TGCGGCAGCC 
AATTGTGTTT ATGAGTTGAT GGCTGCATTC GGGCTCACTA ATGTGAACGT TCAAACGTTT 
CTACCAGAAT TATTAAAGCA CGTACCTCAA CTAGCCGGTC ATGGTGATAG AAACGTTCGA 
TCACAAACGA TGAATTTGAT TGTCGAGATC TATAAGGTTA CGGGCAACAA TAGCGACTTG 
TTAGAGGAGA TACTGTTTAA AAAATTAAAA CCAATTCAAG TGAAAGATCT TCATAAATTG 
TTTGCAAAAG TTGGTGACGA GCCATCGTCA TCGAAAATGT TATTCGAGTG GGAGAAAAGA 
GAATTGGAAA AGAAAAGAAG CCAAGAGGAG GAAGCCAGGA AAAGAAAGTC CATATTATCG 
AATGATGAGG GGGAATATCA GATAGATAAG GATGGAGATA CATTGATGGG GATGGAAACG 
GATATGCCAC CATCAAAACA GCAAAGTGGA GTTCAAATAG ACACCTTCAG TATGCTACCG 
GAGGAGACGA TCCTCGATAA ACTGCCTAAG GACTTCCAGG AAAGGATAAC GTCGTCCAAA 
TGGAAAGATA GAGTGGAAGC GCTGGAAGAA TTTTGGGATA GTGTTCTATC GCAGACTAAG 
AAGTTAAAAA GCACCTCGCA AAATTATTCC AACTTATTGG GGATATATGG CCATATTATC 
CAGAAAGATG CAAACATTCA AGCTGTTGCA CTAGCCGCTC AATCAGTAGA ATTAATCTGC 
GACAAACTAA AAACACCAGG CTTTTCCAAG GATTATGTTT CACTAGTCTT CACTCCCTTG 
TTAGACAGGA CAAAGGAAAA GAAACCTTCT GTGATAGAGG CCATTAGGAA GGCATTATTA 
ACCATCTGCA AGTATTACGA TCCATTAGCC TCCAGTGGAC GCAATGAAGA TATGTTGAAA 
GATATTTTGG AGCATATGAA GCACAAGACA CCTCAAATCA GAATGGAGTG TACGCAATTG 
TTTAATGCAA GCATGAAAGA AGAAAAAGAT GGATACTCGA CCCTGCAAAG ATACTTGAAG 
GATGAAGTGG TACCAATTGT GATTCAGATC GTCAACGACA CCCAACCTGC TATAAGAACT 
ATTGGATTTG AGAGTTTCGC CATTCTAATT AAAATATTTG GCATGAATAC CTTTGTGAAA 
ACATTAGAGC ACTTGGATAA TTTGAAAAGG AAAAAAATTG AAGAAACGGT CAAAACTCTA 
CCTAATTTCA GTATTGCAAG TGGTAGTACC CATAGCACTA TTGAAACGAA TAAACAAACT 
GGGCCAATGG AGAATAAATT TCTCCTGAAA AAAAGTTCTG TACTTCCCTC CAAGAGAGTG 
GCATCTTCAC CGCTGAGAAA TGATAACAAA AGTAAAGTGA ACCCAATAGG GTCAGTAGCC 
TCCGCATCAA AACCATCAAT GGTAGCTGCT AACAATAAAT CGAGGGTCCT GTTGACGTCT 
AAGTCTTTGG CAACGCCAAA AAACGTAGTA GCTAATAGCA CTGACAAGAA TGAAAAGTTG 
ATTGAAGAAT ACAAGTACAG ACTGCAAAAA CTACAAAATG ATGAGATGAT ATGGACCAAA 
GAAAGACAAA GCCTATTGGA AAAGATGAAT AATACAGAAA ATTATAAGAT CGAAATGATT 
AAAGAAAACG AAATGCTAAG AGAGCAACTA AAGGAAGCGC AGTCTAAATT GAACGAAAAG 
AACATTCAAT TAAGATCCAA GGAGATTGAT GTCAATAAGC TGTCTGATAG AGTACTGTCA 
CTAGAAAACG AGCTTAGAAA CATGGAGATC GAATTGGATA GAAATAAGAA AAGAAATGAT 
ACAAACTTGC AATCGATGGG TACGATTTCA TCATACTCCA TACCTTCTTC CACCGTGTCA 
AGCAACTATG GGGTAAAATC TTTATCTTCC GCTTTGCCAT TTAAGGAGGA GGAAGATGTC 
AGAAGGAAAG AGGACGTCAA CTATGAAAGA AGATCAAGCG AGTCCATCGG CGACCTGCCG 
CACCGCGTAA ACTCTCTTAA CATACGGCCG TACAGAAAAA ACGGCACTGG AGTGAGCAGT 
GTCTCCGACG ATTTGGATAT CGATTTCAAC GATAGTTTTG CCTCAGAAGA AAGTTATAAA 
CGTGCCGCTG CAGTGACATC TACCCTAAAG GCCAGAATTG AAAAAATGAA GGCCAAATCA 
AGGCGGGAAG GGACAACCAG GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013146.1
CDS	1..2667
Translation	MSGEEEVDYTTLPLEERLTYKLWKARLEAYKELNQLFRNSVGDISRDDNIQIYWRDPTLFAQYITDSNVVAQEQAIVALNSLIDAFASSSLKNAHNITLISTWTPLLVEKGLTSSRATTKTQSMSCILSLCGLDTSITQSVELVIPFFEKKLPKLIAAAANCVYELMAAFGLTNVNVQTFLPELLKHVPQLAGHGDRNVRSQTMNLIVEIYKVTGNNSDLLEEILFKKLKPIQVKDLHKLFAKVGDEPSSSKMLFEWEKRELEKKRSQEEEARKRKSILSNDEGEYQIDKDGDTLMGMETDMPPSKQQSGVQIDTFSMLPEETILDKLPKDFQERITSSKWKDRVEALEEFWDSVLSQTKKLKSTSQNYSNLLGIYGHIIQKDANIQAVALAAQSVELICDKLKTPGFSKDYVSLVFTPLLDRTKEKKPSVIEAIRKALLTICKYYDPLASSGRNEDMLKDILEHMKHKTPQIRMECTQLFNASMKEEKDGYSTLQRYLKDEVVPIVIQIVNDTQPAIRTIGFESFAILIKIFGMNTFVKTLEHLDNLKRKKIEETVKTLPNFSIASGSTHSTIETNKQTGPMENKFLLKKSSVLPSKRVASSPLRNDNKSKVNPIGSVASASKPSMVAANNKSRVLLTSKSLATPKNVVANSTDKNEKLIEEYKYRLQKLQNDEMIWTKERQSLLEKMNNTENYKIEMIKENEMLREQLKEAQSKLNEKNIQLRSKEIDVNKLSDRVLSLENELRNMEIELDRNKKRNDTNLQSMGTISSYSIPSSTVSSNYGVKSLSSALPFKEEEDVRRKEDVNYERRSSESIGDLPHRVNSLNIRPYRKNGTGVSSVSDDLDIDFNDSFASEESYKRAAAVTSTLKARIEKMKAKSRREGTTRT

Target ORF information:

RefSeq Version	NM_001181932.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Stu2p (STU2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181932.1

ORF Insert Sequence:

ATGTCAGGAG AAGAAGAAGT AGATTATACA ACGTTACCCC TAGAAGAGCG ATTAACGTAC 
AAGTTATGGA AGGCACGTTT AGAAGCTTAT AAGGAGTTAA ACCAGCTTTT TAGAAACTCC 
GTTGGCGACA TCAGTAGGGA TGACAACATA CAAATTTACT GGAGGGATCC TACATTATTT 
GCACAATATA TAACTGATTC TAATGTGGTC GCTCAAGAAC AAGCCATTGT TGCCTTGAAT 
AGTTTAATAG ATGCCTTTGC GTCATCGTCT TTGAAAAATG CACATAATAT TACTTTAATA 
TCAACATGGA CACCATTGCT TGTGGAAAAG GGCTTAACGT CCTCAAGGGC TACAACGAAG 
ACGCAGTCGA TGAGCTGTAT ACTCTCACTA TGTGGTCTTG ATACATCTAT TACGCAATCG 
GTGGAATTGG TAATACCCTT TTTCGAGAAA AAACTGCCAA AATTAATTGC TGCGGCAGCC 
AATTGTGTTT ATGAGTTGAT GGCTGCATTC GGGCTCACTA ATGTGAACGT TCAAACGTTT 
CTACCAGAAT TATTAAAGCA CGTACCTCAA CTAGCCGGTC ATGGTGATAG AAACGTTCGA 
TCACAAACGA TGAATTTGAT TGTCGAGATC TATAAGGTTA CGGGCAACAA TAGCGACTTG 
TTAGAGGAGA TACTGTTTAA AAAATTAAAA CCAATTCAAG TGAAAGATCT TCATAAATTG 
TTTGCAAAAG TTGGTGACGA GCCATCGTCA TCGAAAATGT TATTCGAGTG GGAGAAAAGA 
GAATTGGAAA AGAAAAGAAG CCAAGAGGAG GAAGCCAGGA AAAGAAAGTC CATATTATCG 
AATGATGAGG GGGAATATCA GATAGATAAG GATGGAGATA CATTGATGGG GATGGAAACG 
GATATGCCAC CATCAAAACA GCAAAGTGGA GTTCAAATAG ACACCTTCAG TATGCTACCG 
GAGGAGACGA TCCTCGATAA ACTGCCTAAG GACTTCCAGG AAAGGATAAC GTCGTCCAAA 
TGGAAAGATA GAGTGGAAGC GCTGGAAGAA TTTTGGGATA GTGTTCTATC GCAGACTAAG 
AAGTTAAAAA GCACCTCGCA AAATTATTCC AACTTATTGG GGATATATGG CCATATTATC 
CAGAAAGATG CAAACATTCA AGCTGTTGCA CTAGCCGCTC AATCAGTAGA ATTAATCTGC 
GACAAACTAA AAACACCAGG CTTTTCCAAG GATTATGTTT CACTAGTCTT CACTCCCTTG 
TTAGACAGGA CAAAGGAAAA GAAACCTTCT GTGATAGAGG CCATTAGGAA GGCATTATTA 
ACCATCTGCA AGTATTACGA TCCATTAGCC TCCAGTGGAC GCAATGAAGA TATGTTGAAA 
GATATTTTGG AGCATATGAA GCACAAGACA CCTCAAATCA GAATGGAGTG TACGCAATTG 
TTTAATGCAA GCATGAAAGA AGAAAAAGAT GGATACTCGA CCCTGCAAAG ATACTTGAAG 
GATGAAGTGG TACCAATTGT GATTCAGATC GTCAACGACA CCCAACCTGC TATAAGAACT 
ATTGGATTTG AGAGTTTCGC CATTCTAATT AAAATATTTG GCATGAATAC CTTTGTGAAA 
ACATTAGAGC ACTTGGATAA TTTGAAAAGG AAAAAAATTG AAGAAACGGT CAAAACTCTA 
CCTAATTTCA GTATTGCAAG TGGTAGTACC CATAGCACTA TTGAAACGAA TAAACAAACT 
GGGCCAATGG AGAATAAATT TCTCCTGAAA AAAAGTTCTG TACTTCCCTC CAAGAGAGTG 
GCATCTTCAC CGCTGAGAAA TGATAACAAA AGTAAAGTGA ACCCAATAGG GTCAGTAGCC 
TCCGCATCAA AACCATCAAT GGTAGCTGCT AACAATAAAT CGAGGGTCCT GTTGACGTCT 
AAGTCTTTGG CAACGCCAAA AAACGTAGTA GCTAATAGCA CTGACAAGAA TGAAAAGTTG 
ATTGAAGAAT ACAAGTACAG ACTGCAAAAA CTACAAAATG ATGAGATGAT ATGGACCAAA 
GAAAGACAAA GCCTATTGGA AAAGATGAAT AATACAGAAA ATTATAAGAT CGAAATGATT 
AAAGAAAACG AAATGCTAAG AGAGCAACTA AAGGAAGCGC AGTCTAAATT GAACGAAAAG 
AACATTCAAT TAAGATCCAA GGAGATTGAT GTCAATAAGC TGTCTGATAG AGTACTGTCA 
CTAGAAAACG AGCTTAGAAA CATGGAGATC GAATTGGATA GAAATAAGAA AAGAAATGAT 
ACAAACTTGC AATCGATGGG TACGATTTCA TCATACTCCA TACCTTCTTC CACCGTGTCA 
AGCAACTATG GGGTAAAATC TTTATCTTCC GCTTTGCCAT TTAAGGAGGA GGAAGATGTC 
AGAAGGAAAG AGGACGTCAA CTATGAAAGA AGATCAAGCG AGTCCATCGG CGACCTGCCG 
CACCGCGTAA ACTCTCTTAA CATACGGCCG TACAGAAAAA ACGGCACTGG AGTGAGCAGT 
GTCTCCGACG ATTTGGATAT CGATTTCAAC GATAGTTTTG CCTCAGAAGA AAGTTATAAA 
CGTGCCGCTG CAGTGACATC TACCCTAAAG GCCAGAATTG AAAAAATGAA GGCCAAATCA 
AGGCGGGAAG GGACAACCAG GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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STU2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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