alp14 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	alp14
Entrez Gene ID	2539497
Full Name	microtubule polymerase alp14
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023040.2	NP_588048.1	microtubule polymerase alp14

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	alp14	NP_588048.1
Kluyveromyces lactis	KLLA0E16325g	XP_454685.1
Saccharomyces cerevisiae (baker's yeast)	STU2	NP_013146.1
Eremothecium gossypii	AGOS_ACL132C	NP_983272.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ability of Alp7/14 to accelerate and bias GTP-tubulin exchange at microtubule plus ends allows it to generate long-lived, fast-growing microtubules at very low cellular free tubulin concentrations.
Title: Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.

CDK phosphorylates the Alp7-Alp14 complex to localize it to the nucleus.
Title: CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly.

An Ndc80 loop plays sequential roles in spindle-kinetochore attachment by connecting the Ndc80 complex to Dis1/TOG and Alp7/TACC-Alp14/TOG.
Title: The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.

studies suggest that Alp14 regulates the polymerization state of tubulin by cycling between a tubulin dimer-bound cytoplasmic state and a MT polymerase state that promotes rapid MT assembly.
Title: Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase.

The following alp14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alp14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99632	NM_001023040.2 Latest version!	Schizosaccharomyces pombe microtubule polymerase alp14 (alp14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alp14 ( NM_001023040.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588048.1 Schizosaccharomyces pombe microtubule polymerase alp14 (alp14), mRNA.

Price & Availability↑

CloneID	OSc99632
Clone ID Related Accession (Same CDS sequence)	NM_001023040.2
Accession Version	NM_001023040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	microtubule polymerase alp14
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023040.1.

ATGAGCCAAG ATCAAGAAGA AGACTACTCC AAACTTCCTC TAGAATCAAG AATAGTACAT 
AAAGTATGGA AAGTGCGTCT ATCTGCTTAT GAAGAGTGCA GCAAATCGTT TTCACTTTCA 
GCAGATGGTT CGGATAATTG CTTTGAGCTT TGGAATAATC AGAGTGAATT GTGGAAATCA 
GTGCTGACTG ATAGCAACGT AGCAGCTCAA GAAGCTGGTA CCGCAGCGTT TGTAGCTTAT 
TGCAGGTTTT CCGATCCTTC TCATTTACTT AAAGCTCGTG AAATATCAGT CCTTTCAATT 
TCAGAGAAAT GCTTAACATC ACCTCGCGCA GGTACAAGGG AAAATGCACT AGAAGCTCTT 
ATGCTATTAG TTGAAGCAGA TTCAGCTGCG CCAGTTATAG AATCAATCAT TCCTTCTTTA 
TCGGCTCGAT CCCCGAAGGT AATAGCTTCT AATGTCGCTG CCATTGCTAG TCTAGTTGAG 
CAGTTTGGTG CTAAAGTTAT TCCTTCAAAA ATGATTATTC CACATATCTC CAACTTGTTT 
GGTCATGCTG ATAAAAATGT TCGTAAGGAA GCATCTCGTT TAACTGTAAA CATTTATCGG 
TGGACAGGGG ATCCTTTAAA AGATTTACTA TTTAAAGATC TAAGACCAGT TCAGACGAAA 
GAGCTTGAGT CTCTTTTTGC TGAGCTACCA ACAGAGCCTC CCAAACAAAC TCGTTTTTTG 
AAAAGTCAGC AACCTACATC AGAGCCGAAT GTTGAAACAC AGGTAGAAGA GCAACCTGCT 
CTCGAGAATG AAGAAAGTGA ACCAGAACCG TCAGATGATC AATTTGATTT GGTAGAAGAA 
GTGGACGTTT TACCCAATGT GGATCCTAAC CTGGAAACTT TAATGGCTTC TTCGAAATGG 
AAGGATCGTA AGGAAGCGTT AGACAAACTA CTTCCTGTCT TATCTCAACC AAAGATCAAA 
GACAACGATT TTTTTAATTT AGTTGCTATT CTCACAAAGT CTGTTTCAAA AGACGCAAAT 
ATCATGGTGG TTATAAATGC TGCACACTGT ATACAAGCGA TGGCTAAAGG TTTACGCTCA 
AATTTTTCGA AGTATGCCTC AACATCTATT AATGCACTGT TGGAACGATC CAAAGAGAAA 
AAGGCTAATG TAATCGAATC ACTATCTTCT GCGATGGATG CTGTTTTGGC TACATCCAGT 
TTGGATGACT TAGCCGAGCT CATTGCTAGC TTTGCCGGGA ACAAAAATCC TCAAATCAAA 
AGCTCCTGCT TTAGTTTGTT CTCGAGATCT TTCAGTAATA TGACATCCTT ACCATCTAAG 
TTTACAGTTG ATACCTGTGC TAAAGCATGC GTTCCTGGTG TTTCTGATAC CTTTGAGCCG 
GTGCGTAGTG CGGCAGCAGA AGCCTTGGGT GTTTTGATGA AACTCGTTGG TGAAAGAGCC 
ATTAATCAGT ATTTAAGTCC GTTGGACGAC ATCAGGAAAT CTAAAATTCG TTCTTTTTAT 
GAAACAGCTA CAGTAAAGGC TAAGGCACCT ACAAAAAAGT CGAAAGTTAA ACCTTCAAAA 
CAAGAAGAAT CAAAAGTCGT CGTACCTTCA AATGCTAAGG CTGTCAAGAA ATCCGTTGTT 
CCTTCTAGTC CAGTTGTACC ATCTCCACGA AAGGCAACTA ACAAATCGCT TTCCATGGAT 
GTATCAAAGG GTAATGCATT TGAGAATGGT CCTTTGTTAC CAAGACCGAC TACGAGGCCA 
GTTTCTCGAG GGCTTTCACG AGGCACATCT TCCAGTTTGC AACAAAAAGT AAAAGCTTCC 
ACTCCTTTGA ATTCGGGAGC ACTTAACGAA ACGGTGCAAA ATTTGAAGAA CATGGAGTTG 
GACGATCCGG CTCCTCAACC GGCTAAGCAT TCACGAGTTG ATCGTTATGA ACACCCTAAA 
GTATTAGAAG ACAATGACTC TACCATTTCG TCTTTAGAAT CTCTGAAGAG AGAAAACGAG 
GAGTTACGAG AGCAGTTGAA AGTTGAACAT GAAGAGAATA TTTCTATGCA AAAACAACTC 
AGTGAATTGA AAGGTGAATT AAATACCTTA CGCAGTGCCA GGAAAGCCTC ACCAATAGGA 
GACAGAAAAC CTGCATTTAT GCGAAGAGCT AATACTGACT TTTTAGAGCT CTCTACGAGT 
CCCTCATTTC AACGTTCAGT GAGGGAATTT GAACCTACTA GACCTAAACT TTATAGCTCT 
ATAGATGTGA ATCAGAGATC CCCTTTAGCA TCTGCGAAAA CAAATGGTAA TTTCACGTTT 
CATGCTGAAT TACCAAGATC ACCGTTTTCG TCAAGGGCCA ATAATATCAA TCCTGATTGG 
ACTAAAGCCA TTGATTTAGC TGCCAAACTA AAACAAAAAA TTACTGAGAT GAAACAAACA 
GACCAAAGAC ATCAGGGATT GATTCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588048.1
CDS	351..2780
Translation	MSQDQEEDYSKLPLESRIVHKVWKVRLSAYEECSKSFSLSADGSDNCFELWNNQSELWKSVLTDSNVAAQEAGTAAFVAYCRFSDPSHLLKAREISVLSISEKCLTSPRAGTRENALEALMLLVEADSAAPVIESIIPSLSARSPKVIASNVAAIASLVEQFGAKVIPSKMIIPHISNLFGHADKNVRKEASRLTVNIYRWTGDPLKDLLFKDLRPVQTKELESLFAELPTEPPKQTRFLKSQQPTSEPNVETQVEEQPALENEESEPEPSDDQFDLVEEVDVLPNVDPNLETLMASSKWKDRKEALDKLLPVLSQPKIKDNDFFNLVAILTKSVSKDANIMVVINAAHCIQAMAKGLRSNFSKYASTSINALLERSKEKKANVIESLSSAMDAVLATSSLDDLAELIASFAGNKNPQIKSSCFSLFSRSFSNMTSLPSKFTVDTCAKACVPGVSDTFEPVRSAAAEALGVLMKLVGERAINQYLSPLDDIRKSKIRSFYETATVKAKAPTKKSKVKPSKQEESKVVVPSNAKAVKKSVVPSSPVVPSPRKATNKSLSMDVSKGNAFENGPLLPRPTTRPVSRGLSRGTSSSLQQKVKASTPLNSGALNETVQNLKNMELDDPAPQPAKHSRVDRYEHPKVLEDNDSTISSLESLKRENEELREQLKVEHEENISMQKQLSELKGELNTLRSARKASPIGDRKPAFMRRANTDFLELSTSPSFQRSVREFEPTRPKLYSSIDVNQRSPLASAKTNGNFTFHAELPRSPFSSRANNINPDWTKAIDLAAKLKQKITEMKQTDQRHQGLIH

Target ORF information:

RefSeq Version	NM_001023040.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe microtubule polymerase alp14 (alp14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023040.2

ORF Insert Sequence:

ATGAGCCAAG ATCAAGAAGA AGACTACTCC AAACTTCCTC TAGAATCAAG AATAGTACAT 
AAAGTATGGA AAGTGCGTCT ATCTGCTTAT GAAGAGTGCA GCAAATCGTT TTCACTTTCA 
GCAGATGGTT CGGATAATTG CTTTGAGCTT TGGAATAATC AGAGTGAATT GTGGAAATCA 
GTGCTGACTG ATAGCAACGT AGCAGCTCAA GAAGCTGGTA CCGCAGCGTT TGTAGCTTAT 
TGCAGGTTTT CCGATCCTTC TCATTTACTT AAAGCTCGTG AAATATCAGT CCTTTCAATT 
TCAGAGAAAT GCTTAACATC ACCTCGCGCA GGTACAAGGG AAAATGCACT AGAAGCTCTT 
ATGCTATTAG TTGAAGCAGA TTCAGCTGCG CCAGTTATAG AATCAATCAT TCCTTCTTTA 
TCGGCTCGAT CCCCGAAGGT AATAGCTTCT AATGTCGCTG CCATTGCTAG TCTAGTTGAG 
CAGTTTGGTG CTAAAGTTAT TCCTTCAAAA ATGATTATTC CACATATCTC CAACTTGTTT 
GGTCATGCTG ATAAAAATGT TCGTAAGGAA GCATCTCGTT TAACTGTAAA CATTTATCGG 
TGGACAGGGG ATCCTTTAAA AGATTTACTA TTTAAAGATC TAAGACCAGT TCAGACGAAA 
GAGCTTGAGT CTCTTTTTGC TGAGCTACCA ACAGAGCCTC CCAAACAAAC TCGTTTTTTG 
AAAAGTCAGC AACCTACATC AGAGCCGAAT GTTGAAACAC AGGTAGAAGA GCAACCTGCT 
CTCGAGAATG AAGAAAGTGA ACCAGAACCG TCAGATGATC AATTTGATTT GGTAGAAGAA 
GTGGACGTTT TACCCAATGT GGATCCTAAC CTGGAAACTT TAATGGCTTC TTCGAAATGG 
AAGGATCGTA AGGAAGCGTT AGACAAACTA CTTCCTGTCT TATCTCAACC AAAGATCAAA 
GACAACGATT TTTTTAATTT AGTTGCTATT CTCACAAAGT CTGTTTCAAA AGACGCAAAT 
ATCATGGTGG TTATAAATGC TGCACACTGT ATACAAGCGA TGGCTAAAGG TTTACGCTCA 
AATTTTTCGA AGTATGCCTC AACATCTATT AATGCACTGT TGGAACGATC CAAAGAGAAA 
AAGGCTAATG TAATCGAATC ACTATCTTCT GCGATGGATG CTGTTTTGGC TACATCCAGT 
TTGGATGACT TAGCCGAGCT CATTGCTAGC TTTGCCGGGA ACAAAAATCC TCAAATCAAA 
AGCTCCTGCT TTAGTTTGTT CTCGAGATCT TTCAGTAATA TGACATCCTT ACCATCTAAG 
TTTACAGTTG ATACCTGTGC TAAAGCATGC GTTCCTGGTG TTTCTGATAC CTTTGAGCCG 
GTGCGTAGTG CGGCAGCAGA AGCCTTGGGT GTTTTGATGA AACTCGTTGG TGAAAGAGCC 
ATTAATCAGT ATTTAAGTCC GTTGGACGAC ATCAGGAAAT CTAAAATTCG TTCTTTTTAT 
GAAACAGCTA CAGTAAAGGC TAAGGCACCT ACAAAAAAGT CGAAAGTTAA ACCTTCAAAA 
CAAGAAGAAT CAAAAGTCGT CGTACCTTCA AATGCTAAGG CTGTCAAGAA ATCCGTTGTT 
CCTTCTAGTC CAGTTGTACC ATCTCCACGA AAGGCAACTA ACAAATCGCT TTCCATGGAT 
GTATCAAAGG GTAATGCATT TGAGAATGGT CCTTTGTTAC CAAGACCGAC TACGAGGCCA 
GTTTCTCGAG GGCTTTCACG AGGCACATCT TCCAGTTTGC AACAAAAAGT AAAAGCTTCC 
ACTCCTTTGA ATTCGGGAGC ACTTAACGAA ACGGTGCAAA ATTTGAAGAA CATGGAGTTG 
GACGATCCGG CTCCTCAACC GGCTAAGCAT TCACGAGTTG ATCGTTATGA ACACCCTAAA 
GTATTAGAAG ACAATGACTC TACCATTTCG TCTTTAGAAT CTCTGAAGAG AGAAAACGAG 
GAGTTACGAG AGCAGTTGAA AGTTGAACAT GAAGAGAATA TTTCTATGCA AAAACAACTC 
AGTGAATTGA AAGGTGAATT AAATACCTTA CGCAGTGCCA GGAAAGCCTC ACCAATAGGA 
GACAGAAAAC CTGCATTTAT GCGAAGAGCT AATACTGACT TTTTAGAGCT CTCTACGAGT 
CCCTCATTTC AACGTTCAGT GAGGGAATTT GAACCTACTA GACCTAAACT TTATAGCTCT 
ATAGATGTGA ATCAGAGATC CCCTTTAGCA TCTGCGAAAA CAAATGGTAA TTTCACGTTT 
CATGCTGAAT TACCAAGATC ACCGTTTTCG TCAAGGGCCA ATAATATCAA TCCTGATTGG 
ACTAAAGCCA TTGATTTAGC TGCCAAACTA AAACAAAAAA TTACTGAGAT GAAACAAACA 
GACCAAAGAC ATCAGGGATT GATTCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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alp14 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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