FAB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FAB1
Entrez Gene ID	850574
Full Name	1-phosphatidylinositol-3-phosphate 5-kinase
Synonyms	SVL7
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179984.2	NP_116674.2	1-phosphatidylinositol-3-phosphate 5-kinase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	FAB1	NP_116674.2
Eremothecium gossypii	AGOS_AER188C	NP_985045.2
Kluyveromyces lactis	KLLA0D04598g	XP_453267.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pho85/CDK5 provides protection against hyperosmotic stress and acts before long-term adaptation provided by Hog1. This protection requires the vacuolar/endolysosomal signaling lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells.
Title: Early protection to stress mediated by CDK-dependent PI3,5P

Data suggest a model in which PtdIns(3)P 5-kinase protein Fab1/PIKfyve is dispensable to maintain vacuolar/lysosomal acidification.
Title: The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole.

ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
Title: ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.

Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct
Title: Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function.

The crystal structure now unravels the presence of two distinct phosphoinositide-binding sites at the circumference of the seven bladed beta-propeller showing PtdINS3P and PtdIns(3,5)P2 proteins
Title: It takes two to tango: PROPPINs use two phosphoinositide-binding sites.

The following FAB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00256	NM_001179984.2 Latest version!	Saccharomyces cerevisiae S288C 1-phosphatidylinositol-3-phosphate 5-kinase (FAB1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAB1 ( NM_001179984.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116674.2 Saccharomyces cerevisiae S288C 1-phosphatidylinositol-3-phosphate 5-kinase (FAB1), partial mRNA.

Price & Availability↑

CloneID	OSi00256
Clone ID Related Accession (Same CDS sequence)	NM_001179984.2
Accession Version	NM_001179984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	1-phosphatidylinositol-3-phosphate 5-kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). On Apr 26, 2011 this sequence version replaced NM_001179984.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATCGG AAGAACCCCA TGCATCTATA TCCTTTCCAG ATGGCTCACA TGTCCGGTCG 
TCCTCCACTG GTACTTCATC TGTGAACACA ATCGATGCTA CCTTATCCAG ACCGAACTAT 
ATTAAGAAAC CGTCCCTGCA CATTATGTCG ACATCTACGA CGTCGACTAC GACGGATCTA 
GTGACAAACC CAATTCTGAG CAATATCTCC GTGCCAAAAA TAAGCCCCCC CACATCATCC 
TCGATAGCAA CAGCGACATC AACAAGTCAC GTGACCGGCA CTGCGTCGCA TTCGAACATT 
AAAGCTAATG CTAACACTAG TACCAGCGTG AATAAGAAAA ATTTGCCGCC AACAACTAGT 
GGGAGGATAC CCTCTAGCAC CATCAAGAGA TATCCGAGTC GCTATAAGCC TTCTCACTCG 
CTGCAGCTGC CCATTAAGAA CGACTCCAAC TTTAAACGAT CTAGCATATA TGCCTCCAAA 
TCCACTGTTA CTGCTATCCC CATTAGGAAC AATCGGCCAA TTTCCATGCA GAATTCCTAT 
GCGCGCACTC CTGACAGCGA TCATGACGAC GTTGGCGATG AAGTGTCTTC GATAAAGAGT 
GCCTCTTCTT CGTTGACCGC ATCCCTGTCC AAGAGTTTCC TGTTCGCATT TTATAACAAC 
CGCAAGAAGG ACAAGACCAG TAACAATGGT GTCCTCTCAA AGGAATATTG GATGAAGGAC 
GAGAGTTCTA AAGAATGTTT CAGTTGTGGT AAAACGTTTA ACACTTTCAG AAGGAAACAC 
CACTGCAGGA TATGCGGCCA AATTTTTTGC AGCAGTTGCA CTCTCTTGAT AGATGGTGAT 
CGATTCGGCT GTCATGCCAA GATGCGAGTA TGTTACAATT GTTATGAGCA TGCAGACACA 
TACGAGGACT CCAGTGATGA AGAGAATGAT TCTACAATGC AATTGAATGA GCCAAGGTCT 
CGCTCTCGCT CTCGTTCTTC CAATACCAAT CCATATTCTC ATTCACATTC GCATTTGCAT 
CTCATTTCAC AGGACAACCA TAACGGTACC GATCTCCATG ATCCAGTAGC AGCAACGGAT 
AACCCTCAAC AGCAGAATGA GGTCTACCTA TTAAATGACG ATGACGTACA AAGTATAATG 
ACCTCAGGTG AAGATTCTAA ATTGTTCATT TCTACACCCC CACCACCACC GAAGATGGCC 
ATCCCAGCAA CAAAGCAAGG CGGATCGCTG GAAATCTCAT TCGATTCGGA AAACGATAGG 
GCTTTGCATT ATCAAGATGA CAACCCCGGT CGCCATCATC ACCTCGATTC CGTGCCCACA 
AGGTACACTA TCAGAGATAT GGACAATATA TCACATTACG ATACCAACTC TAATTCCACT 
TTGAGGCCAC ATTATAACAC CAATAACAGC ACTATTACCA TCAACAACCT CAACAACACT 
ACTTCCAACA ACAGCAACTA TAACAATACC AATAGCAACA GCAACATCAA TAATCCTGCG 
CATTCCTTAA GAAGGTCTAT TTTCCATTAT GTAAGCAGCA ATAGTGTTAA CAAGGACAGT 
AACAATAGCT CTGCAACACC CGCTTCGTCT GCACAGTCTT CTTCCATACT GGATCCTGCC 
AATCGTATTA TCGGGAATTA TGCTCATAGA AATTACAAGT TTAAATTCAA CTACAATTCC 
AAAGGTCCTT CGCAACAAAA TGACACGGCA AATGGAAATA ATGATAATAA TAATAATAAT 
AATAATAATA ATAATAATAA TAATAATAAC AGTGCCAGCG GTATTGCTGA CAACAATAAC 
ATTCCCTCAA ACGACAATGG CACGACGTTC ACTCTAGATA AGAAGAAAAG AAACCCACTG 
ACAAAATCTA AATCTACGTC CGCTTACCTA GAATATCCTC TAAACGAGGA AGACTCTTCT 
GAGGACGAAG GTTCCATGTC CATATATTCT GTATTGAATG ATGACCATAA AACTGACAAT 
CCAATTAGAT CAATGAGGAA CTCCACAAAA TCTTTTCAAC GAGCGCAAGC TTCACTACAG 
CGGATGAGGT TTAGAAGGAA AAGTAAAAGC AAGCATTTTC CCAACAATTC GAAAAGTTCC 
ATTTATAGAG ACTTAAATTT CTTGACCAAT AGTACTCCCA ATTTACTTTC TGTAGTAAGC 
GACGATAATC TATACGACGA TTCTTCTCCT TTGCAAGACA AGGCCTCTTC ATCCGCTGCA 
AGCAGACTCA CTGACAGAAA ATTCAGCAAT AGCAGTGGCA GCAATAATAA TAGCAATAGT 
AATAGCAATA TCAACACCGA TCCTTGGAAG AGAATCGCTA GTATATCGGG ATTCAAACTG 
AAAAAGGAAA AAAAGAGAGA ATTAAATGAA GTCTCTCTTT TACATATGCA TGCCCTATTA 
AAACAATTAT TGAATGATCA AGAAATTTCC AATCTTCAAG AATGGATAAC CCTTCTAGAT 
GGCGCTTTGC GTAAAGTTTT AAGGACCATC CTGAACGCGA GGGATTTGAA CACATTAGAT 
TTTAGACAAA CTTACGTTAA GATAAAAAGA ATTAGTGGTG GATCTCCTCA AAATTCAGAA 
TATATCGACG GGGTTGTCTT TTCGAAAGCA TTGCCGTCGA AAACAATGCC ACGGCATTTG 
AAAAACCCAA GGATACTATT AATCATGTTT CCACTGGAGT ACCAAAAGAA CAATAACCAC 
TTTCTAAGTA TAGAGTCCGT CTTTAGGCAA GAGCGCGAAT ATTTGGATAA ACTTGTTTCG 
AGATTAAAGT CATTACATCC TGATATTATA TACGTCGGCG CCAATGTTAG TGGGTACGCG 
TTAGAACTAT TAAATGACTC AGGCATTGTC GTACAGTTTA ATATGAAACC ACAAGTCATT 
GAAAGAATTG CCAAGCTAAC AGAAGCAGAT ATTGCCATTT CTGTGGATAA GTTGGCTACG 
AATATTAAGA TGGGTGAATG TGAAACTTTT GAAGTTAAAA GCTACATTTA TGGAAACATC 
AGTAAAACAT ATACATTTCT TCGGGGCTGT AACCCAGAAC TTGGTGGAAC AATTTTGTTA 
AGAGGTGATA GTCTTGAAAA CTTGAGAAAA ATCAAACAAG TCTCTGAATT TATGGTCTAC 
GCAATTTTTT CATTGAAGTT GGAAAGTTCA TTTTTTAACG ATAATTTCAT ACAACTTTCT 
ACAGATGTTT ATTTGAAGAG AGCTGAATCC AAAAAACTGC AAGTTTTCGA AGGTTATTTT 
GCAGATTTCT TGATCAAATT CAATAACAGG ATTTTGACTG TATCACCAAC GGTAGATTTC 
CCCATTCCCT TTTTATTGGA AAAAGCCAGA GGCTTGGAAA AGAAGCTCAT AGAAAGGATC 
AATCAATATG AAAGCGAATC TGATTTAGAT CGGCAAACGC AACTAAATAT GCTTCAAGGC 
TTAGAATCGA CTATCACTAA GAAACACTTA GGAAATTTAA TAAAATTCCT GCATGAAATG 
GAAATTGAAA ATTTAGAGCT TGAGTTTCAG AAGAGAAGTC GTCAATGGGA GGTTTCATAT 
TCATCATCAC AGAACTTACT TGGTACGGGC TCACACCAGT CTATAACAGT TCTCTACTCA 
ATGGTTTCAA CAAAGACTGC CACACCTTGC GTTGGCCCTC AAATTGTTAC TATTGACTAT 
TTTTGGGACA GTGATATTTC CATTGGTCAG TTTATCGAGA ACGTCGTGGG AACTGCCAGG 
TATCCCTGCC AACAAGGCTG TAATGGTCTG TATTTGGATC ATTATAGAAG CTATGTTCAT 
GGGTCAGGAA AAGTAGACGT TTTAATCGAA AAATTCCAAA CTAGATTACC AAAATTAAAA 
GATATTATCC TCACGTGGAG TTATTGTAAA AAATGTGGGA CATCTACTCC GATTTTACAA 
ATTAGCGAAA AAACCTGGAA TCATTCTTTC GGTAAATATT TAGAAGTCAT GTTTTGGAGT 
TACAAAGATA GCGTGACAGG AATTGGGAAA TGTCCTCATG ATTTCACTAA AGATCATGTC 
AAATACTTTG GTTACAATGA TTTAGTGGTT CGACTAGAAT ATTCTGATTT AGAAGTTCAT 
GAATTGATAA CCCCACCACG CAAAATAAAA TGGAAACCTC ATATCGACAT CAAACTGAAG 
GTGGAACTAT ACTATAAGAT TCTGGAAAAG ATCAATAATT TCTATGGGAG TGTCCTAAGT 
CGGTTGGAAC GTATTAAATT GGATAGTATG ACTAAAGATA AAGTTCTTAG TGGACAAGCT 
AAAATTATCG AATTGAAGAG CAATGCTACA GAGGAGCAAA AATTAATGTT GCAAGACCTG 
GATACTTTTT ACGCAGATAG TCCTTGCGAT CAACATTTAC CATTGAACCT GGTTATCAAA 
TCTCTATACG ATAAGGCTGT AAATTGGAAC TCTACATTTG CGATATTTGC AAAAAGCTAT 
TTGCCTTCTG AAACTGATAT TAGTCGTATC ACGGCCAAGC AGCTGAAAAA ACTGTTTTAC 
GATTCTTCAA GAAAAGACAG CGAAGACAAA AAATCACTTC ATGATGAAAA GGCGAAGACG 
AGGAAACCAG AAAAGAACGA ATTACCGTTA GAGGGATTGA AAGATGTCGA AAAGCCGAAA 
ATTGATAGCA AGAATACGAC AGAGAATCGT GACAGAACGA ACGAACCACA GAATGCCGTG 
ACGATAACCA CTTTTAAAGA CGACACTCCA ATTATTCCAA CAAGTGGTAC TAGTCACTTG 
ACAGTTACAC CTTCTGCATC GTCGGTTTCT TCTTCTTTGA CCCCTCAAAC CGAAGAACGT 
CCGCCAATAA GCAGAAGTGG AACAGGTATA TCTATGACTC ATGATAAAAG CACTAGACCT 
AACATAAGAA AAATGAGTAG TGATAGTAGT TTATGTGGAC TAGCATCACT TGCTAATGAA 
TATAGTAAAA ATAATAAGGT GAGTAAGCTA GCTACATTTT TCGATCAAAT GCATTTTGAT 
GCCCTGTCGA AAGAATTTGA ACTGGAACGT GAAAGGGAAA GATTACAACT TAATAAGGAT 
AAATACCAAG CCATACGATT ACAAACATCC ACACCAATTG TGGAAATTTA TAAAAATGTC 
AAAGACGCAG TTGATGAACC GCTTCACTCT AGAAGTTCAG GCAATAATTT GTCATCTGCG 
AATGTTAAGA CTTTGGAGGC ACCTGTGGGA GAACATTCCC GGGCCAATAA TTGTAATCCT 
CCTAATTTAG ATCAAAACTT GGAAACTGAA TTAGAAAATT CCATTAGCCA ATGGGGAGAA 
AACATTTTGA ACCCCTCTGG AAAGACCACC GCAAGTACTC ATTTAAATTC TAAGCCAGTA 
GTAAAGGAAA CTTCAGAAAA TCCAAAGTCA ATAGTTAGAG AATCAGATAA TTCAAAGTCG 
GAGCCGCTTC CACCTGTAAT AACAACAACA ACTGTCAATA AGGTAGAATC CACTCCACAA 
CCTGAAAAAT CATTATTGAT GAAAACCTTG AGCAACTTTT GGGCAGATCG ATCTGCGTAT 
TTGTGGAAAC CCCTTGTTTA TCCAACCTGT CCTTCGGAAC ACATTTTTAC TGATAGTGAC 
GTTATAATCC GCGAAGATGA ACCAAGTTCC TTGATCGCAT TTTGCTTAAG TACTTCTGAC 
TATAGAAATA AAATGATGAA CTTGAATGTC CAGCAACAAC AGCAGCAGCA AACAGCAGAA 
GCTGCTCCTG CCAAAACAGG AGGAAACAGT GGAGGAACTA CGCAAACCGG GGATCCTTCT 
GTTAACATTT CTCCCTCAGT TTCTACAACT TCTCATAATA AGGGGAGGGA CTCAGAAATT 
TCATCCTTAG TAACCACCAA AGAGGGCTTG TTGAACACCC CTCCCATTGA AGGCGCCCGA 
GATAGGACCC CACAAGAATC ACAAACGCAT TCCCAAGCAA ATTTAGATAC TCTTCAAGAA 
TTAGAGAAAA TTATGACAAA AAAAACAGCC ACACACTTGA GATATCAATT TGAGGAGGGT 
TTAACGGTAA TGTCATGTAA AATTTTCTTC ACAGAACATT TTGACGTGTT TAGAAAGATT 
TGTGATTGTC AAGAAAACTT CATTCAAAGT TTGTCAAGAT GTGTTAAATG GGATTCCAAT 
GGGGGGAAAA GTGGGAGCGG GTTTTTAAAG ACTTTGGATG ATAGATTTAT TATAAAGGAG 
CTGTCACACG CAGAACTGGA AGCCTTCATA AAATTTGCAC CAAGTTATTT TGAATACATG 
GCACAAGCAA TGTTCCATGA TTTGCCCACG ACTTTGGCTA AAGTATTTGG GTTTTATCAA 
ATTCAAGTAA AAAGCTCTAT TTCAAGTTCC AAAAGTTATA AAATGGACGT TATCATTATG 
GAGAACTTAT TTTATGAGAA GAAAACAACA AGAATATTTG ATTTGAAGGG TTCCATGAGA 
AATAGACATG TCGAACAAAC AGGTAAAGCT AATGAAGTGT TGTTGGATGA AAATATGGTA 
GAGTATATTT ACGAATCACC TATTCATGTT CGCGAATATG ACAAGAAACT TTTAAGAGCT 
TCTGTTTGGA ACGACACATT ATTTTTGGCC AAAATGAATG TGATGGATTA TTCCTTAGTC 
ATTGGTATTG ACAATGAAGG GTATACTTTG ACAGTAGGAA TCATTGATTT CATTCGAACG 
TTTACATGGG ACAAAAAACT AGAAAGTTGG GTCAAAGAGA AAGGCCTAGT AGGGGGGGCC 
AGTGTGATCA AGCAGCCTAC CGTTGTTACA CCAAGACAAT ATAAAAAACG TTTTAGGGAA 
GCAATGGAGA GGTATATTTT GATGGTTCCT GATCCGTGGT ATAGGGAAGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116674.2
CDS	1..6837
Translation	MSSEEPHASISFPDGSHVRSSSTGTSSVNTIDATLSRPNYIKKPSLHIMSTSTTSTTTDLVTNPILSNISVPKISPPTSSSIATATSTSHVTGTASHSNIKANANTSTSVNKKNLPPTTSGRIPSSTIKRYPSRYKPSHSLQLPIKNDSNFKRSSIYASKSTVTAIPIRNNRPISMQNSYARTPDSDHDDVGDEVSSIKSASSSLTASLSKSFLFAFYNNRKKDKTSNNGVLSKEYWMKDESSKECFSCGKTFNTFRRKHHCRICGQIFCSSCTLLIDGDRFGCHAKMRVCYNCYEHADTYEDSSDEENDSTMQLNEPRSRSRSRSSNTNPYSHSHSHLHLISQDNHNGTDLHDPVAATDNPQQQNEVYLLNDDDVQSIMTSGEDSKLFISTPPPPPKMAIPATKQGGSLEISFDSENDRALHYQDDNPGRHHHLDSVPTRYTIRDMDNISHYDTNSNSTLRPHYNTNNSTITINNLNNTTSNNSNYNNTNSNSNINNPAHSLRRSIFHYVSSNSVNKDSNNSSATPASSAQSSSILDPANRIIGNYAHRNYKFKFNYNSKGPSQQNDTANGNNDNNNNNNNNNNNNNNNSASGIADNNNIPSNDNGTTFTLDKKKRNPLTKSKSTSAYLEYPLNEEDSSEDEGSMSIYSVLNDDHKTDNPIRSMRNSTKSFQRAQASLQRMRFRRKSKSKHFPNNSKSSIYRDLNFLTNSTPNLLSVVSDDNLYDDSSPLQDKASSSAASRLTDRKFSNSSGSNNNSNSNSNINTDPWKRIASISGFKLKKEKKRELNEVSLLHMHALLKQLLNDQEISNLQEWITLLDGALRKVLRTILNARDLNTLDFRQTYVKIKRISGGSPQNSEYIDGVVFSKALPSKTMPRHLKNPRILLIMFPLEYQKNNNHFLSIESVFRQEREYLDKLVSRLKSLHPDIIYVGANVSGYALELLNDSGIVVQFNMKPQVIERIAKLTEADIAISVDKLATNIKMGECETFEVKSYIYGNISKTYTFLRGCNPELGGTILLRGDSLENLRKIKQVSEFMVYAIFSLKLESSFFNDNFIQLSTDVYLKRAESKKLQVFEGYFADFLIKFNNRILTVSPTVDFPIPFLLEKARGLEKKLIERINQYESESDLDRQTQLNMLQGLESTITKKHLGNLIKFLHEMEIENLELEFQKRSRQWEVSYSSSQNLLGTGSHQSITVLYSMVSTKTATPCVGPQIVTIDYFWDSDISIGQFIENVVGTARYPCQQGCNGLYLDHYRSYVHGSGKVDVLIEKFQTRLPKLKDIILTWSYCKKCGTSTPILQISEKTWNHSFGKYLEVMFWSYKDSVTGIGKCPHDFTKDHVKYFGYNDLVVRLEYSDLEVHELITPPRKIKWKPHIDIKLKVELYYKILEKINNFYGSVLSRLERIKLDSMTKDKVLSGQAKIIELKSNATEEQKLMLQDLDTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIFAKSYLPSETDISRITAKQLKKLFYDSSRKDSEDKKSLHDEKAKTRKPEKNELPLEGLKDVEKPKIDSKNTTENRDRTNEPQNAVTITTFKDDTPIIPTSGTSHLTVTPSASSVSSSLTPQTEERPPISRSGTGISMTHDKSTRPNIRKMSSDSSLCGLASLANEYSKNNKVSKLATFFDQMHFDALSKEFELERERERLQLNKDKYQAIRLQTSTPIVEIYKNVKDAVDEPLHSRSSGNNLSSANVKTLEAPVGEHSRANNCNPPNLDQNLETELENSISQWGENILNPSGKTTASTHLNSKPVVKETSENPKSIVRESDNSKSEPLPPVITTTTVNKVESTPQPEKSLLMKTLSNFWADRSAYLWKPLVYPTCPSEHIFTDSDVIIREDEPSSLIAFCLSTSDYRNKMMNLNVQQQQQQQTAEAAPAKTGGNSGGTTQTGDPSVNISPSVSTTSHNKGRDSEISSLVTTKEGLLNTPPIEGARDRTPQESQTHSQANLDTLQELEKIMTKKTATHLRYQFEEGLTVMSCKIFFTEHFDVFRKICDCQENFIQSLSRCVKWDSNGGKSGSGFLKTLDDRFIIKELSHAELEAFIKFAPSYFEYMAQAMFHDLPTTLAKVFGFYQIQVKSSISSSKSYKMDVIIMENLFYEKKTTRIFDLKGSMRNRHVEQTGKANEVLLDENMVEYIYESPIHVREYDKKLLRASVWNDTLFLAKMNVMDYSLVIGIDNEGYTLTVGIIDFIRTFTWDKKLESWVKEKGLVGGASVIKQPTVVTPRQYKKRFREAMERYILMVPDPWYREGN

Target ORF information:

RefSeq Version	NM_001179984.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 1-phosphatidylinositol-3-phosphate 5-kinase (FAB1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179984.2

ORF Insert Sequence:

ATGTCATCGG AAGAACCCCA TGCATCTATA TCCTTTCCAG ATGGCTCACA TGTCCGGTCG 
TCCTCCACTG GTACTTCATC TGTGAACACA ATCGATGCTA CCTTATCCAG ACCGAACTAT 
ATTAAGAAAC CGTCCCTGCA CATTATGTCG ACATCTACGA CGTCGACTAC GACGGATCTA 
GTGACAAACC CAATTCTGAG CAATATCTCC GTGCCAAAAA TAAGCCCCCC CACATCATCC 
TCGATAGCAA CAGCGACATC AACAAGTCAC GTGACCGGCA CTGCGTCGCA TTCGAACATT 
AAAGCTAATG CTAACACTAG TACCAGCGTG AATAAGAAAA ATTTGCCGCC AACAACTAGT 
GGGAGGATAC CCTCTAGCAC CATCAAGAGA TATCCGAGTC GCTATAAGCC TTCTCACTCG 
CTGCAGCTGC CCATTAAGAA CGACTCCAAC TTTAAACGAT CTAGCATATA TGCCTCCAAA 
TCCACTGTTA CTGCTATCCC CATTAGGAAC AATCGGCCAA TTTCCATGCA GAATTCCTAT 
GCGCGCACTC CTGACAGCGA TCATGACGAC GTTGGCGATG AAGTGTCTTC GATAAAGAGT 
GCCTCTTCTT CGTTGACCGC ATCCCTGTCC AAGAGTTTCC TGTTCGCATT TTATAACAAC 
CGCAAGAAGG ACAAGACCAG TAACAATGGT GTCCTCTCAA AGGAATATTG GATGAAGGAC 
GAGAGTTCTA AAGAATGTTT CAGTTGTGGT AAAACGTTTA ACACTTTCAG AAGGAAACAC 
CACTGCAGGA TATGCGGCCA AATTTTTTGC AGCAGTTGCA CTCTCTTGAT AGATGGTGAT 
CGATTCGGCT GTCATGCCAA GATGCGAGTA TGTTACAATT GTTATGAGCA TGCAGACACA 
TACGAGGACT CCAGTGATGA AGAGAATGAT TCTACAATGC AATTGAATGA GCCAAGGTCT 
CGCTCTCGCT CTCGTTCTTC CAATACCAAT CCATATTCTC ATTCACATTC GCATTTGCAT 
CTCATTTCAC AGGACAACCA TAACGGTACC GATCTCCATG ATCCAGTAGC AGCAACGGAT 
AACCCTCAAC AGCAGAATGA GGTCTACCTA TTAAATGACG ATGACGTACA AAGTATAATG 
ACCTCAGGTG AAGATTCTAA ATTGTTCATT TCTACACCCC CACCACCACC GAAGATGGCC 
ATCCCAGCAA CAAAGCAAGG CGGATCGCTG GAAATCTCAT TCGATTCGGA AAACGATAGG 
GCTTTGCATT ATCAAGATGA CAACCCCGGT CGCCATCATC ACCTCGATTC CGTGCCCACA 
AGGTACACTA TCAGAGATAT GGACAATATA TCACATTACG ATACCAACTC TAATTCCACT 
TTGAGGCCAC ATTATAACAC CAATAACAGC ACTATTACCA TCAACAACCT CAACAACACT 
ACTTCCAACA ACAGCAACTA TAACAATACC AATAGCAACA GCAACATCAA TAATCCTGCG 
CATTCCTTAA GAAGGTCTAT TTTCCATTAT GTAAGCAGCA ATAGTGTTAA CAAGGACAGT 
AACAATAGCT CTGCAACACC CGCTTCGTCT GCACAGTCTT CTTCCATACT GGATCCTGCC 
AATCGTATTA TCGGGAATTA TGCTCATAGA AATTACAAGT TTAAATTCAA CTACAATTCC 
AAAGGTCCTT CGCAACAAAA TGACACGGCA AATGGAAATA ATGATAATAA TAATAATAAT 
AATAATAATA ATAATAATAA TAATAATAAC AGTGCCAGCG GTATTGCTGA CAACAATAAC 
ATTCCCTCAA ACGACAATGG CACGACGTTC ACTCTAGATA AGAAGAAAAG AAACCCACTG 
ACAAAATCTA AATCTACGTC CGCTTACCTA GAATATCCTC TAAACGAGGA AGACTCTTCT 
GAGGACGAAG GTTCCATGTC CATATATTCT GTATTGAATG ATGACCATAA AACTGACAAT 
CCAATTAGAT CAATGAGGAA CTCCACAAAA TCTTTTCAAC GAGCGCAAGC TTCACTACAG 
CGGATGAGGT TTAGAAGGAA AAGTAAAAGC AAGCATTTTC CCAACAATTC GAAAAGTTCC 
ATTTATAGAG ACTTAAATTT CTTGACCAAT AGTACTCCCA ATTTACTTTC TGTAGTAAGC 
GACGATAATC TATACGACGA TTCTTCTCCT TTGCAAGACA AGGCCTCTTC ATCCGCTGCA 
AGCAGACTCA CTGACAGAAA ATTCAGCAAT AGCAGTGGCA GCAATAATAA TAGCAATAGT 
AATAGCAATA TCAACACCGA TCCTTGGAAG AGAATCGCTA GTATATCGGG ATTCAAACTG 
AAAAAGGAAA AAAAGAGAGA ATTAAATGAA GTCTCTCTTT TACATATGCA TGCCCTATTA 
AAACAATTAT TGAATGATCA AGAAATTTCC AATCTTCAAG AATGGATAAC CCTTCTAGAT 
GGCGCTTTGC GTAAAGTTTT AAGGACCATC CTGAACGCGA GGGATTTGAA CACATTAGAT 
TTTAGACAAA CTTACGTTAA GATAAAAAGA ATTAGTGGTG GATCTCCTCA AAATTCAGAA 
TATATCGACG GGGTTGTCTT TTCGAAAGCA TTGCCGTCGA AAACAATGCC ACGGCATTTG 
AAAAACCCAA GGATACTATT AATCATGTTT CCACTGGAGT ACCAAAAGAA CAATAACCAC 
TTTCTAAGTA TAGAGTCCGT CTTTAGGCAA GAGCGCGAAT ATTTGGATAA ACTTGTTTCG 
AGATTAAAGT CATTACATCC TGATATTATA TACGTCGGCG CCAATGTTAG TGGGTACGCG 
TTAGAACTAT TAAATGACTC AGGCATTGTC GTACAGTTTA ATATGAAACC ACAAGTCATT 
GAAAGAATTG CCAAGCTAAC AGAAGCAGAT ATTGCCATTT CTGTGGATAA GTTGGCTACG 
AATATTAAGA TGGGTGAATG TGAAACTTTT GAAGTTAAAA GCTACATTTA TGGAAACATC 
AGTAAAACAT ATACATTTCT TCGGGGCTGT AACCCAGAAC TTGGTGGAAC AATTTTGTTA 
AGAGGTGATA GTCTTGAAAA CTTGAGAAAA ATCAAACAAG TCTCTGAATT TATGGTCTAC 
GCAATTTTTT CATTGAAGTT GGAAAGTTCA TTTTTTAACG ATAATTTCAT ACAACTTTCT 
ACAGATGTTT ATTTGAAGAG AGCTGAATCC AAAAAACTGC AAGTTTTCGA AGGTTATTTT 
GCAGATTTCT TGATCAAATT CAATAACAGG ATTTTGACTG TATCACCAAC GGTAGATTTC 
CCCATTCCCT TTTTATTGGA AAAAGCCAGA GGCTTGGAAA AGAAGCTCAT AGAAAGGATC 
AATCAATATG AAAGCGAATC TGATTTAGAT CGGCAAACGC AACTAAATAT GCTTCAAGGC 
TTAGAATCGA CTATCACTAA GAAACACTTA GGAAATTTAA TAAAATTCCT GCATGAAATG 
GAAATTGAAA ATTTAGAGCT TGAGTTTCAG AAGAGAAGTC GTCAATGGGA GGTTTCATAT 
TCATCATCAC AGAACTTACT TGGTACGGGC TCACACCAGT CTATAACAGT TCTCTACTCA 
ATGGTTTCAA CAAAGACTGC CACACCTTGC GTTGGCCCTC AAATTGTTAC TATTGACTAT 
TTTTGGGACA GTGATATTTC CATTGGTCAG TTTATCGAGA ACGTCGTGGG AACTGCCAGG 
TATCCCTGCC AACAAGGCTG TAATGGTCTG TATTTGGATC ATTATAGAAG CTATGTTCAT 
GGGTCAGGAA AAGTAGACGT TTTAATCGAA AAATTCCAAA CTAGATTACC AAAATTAAAA 
GATATTATCC TCACGTGGAG TTATTGTAAA AAATGTGGGA CATCTACTCC GATTTTACAA 
ATTAGCGAAA AAACCTGGAA TCATTCTTTC GGTAAATATT TAGAAGTCAT GTTTTGGAGT 
TACAAAGATA GCGTGACAGG AATTGGGAAA TGTCCTCATG ATTTCACTAA AGATCATGTC 
AAATACTTTG GTTACAATGA TTTAGTGGTT CGACTAGAAT ATTCTGATTT AGAAGTTCAT 
GAATTGATAA CCCCACCACG CAAAATAAAA TGGAAACCTC ATATCGACAT CAAACTGAAG 
GTGGAACTAT ACTATAAGAT TCTGGAAAAG ATCAATAATT TCTATGGGAG TGTCCTAAGT 
CGGTTGGAAC GTATTAAATT GGATAGTATG ACTAAAGATA AAGTTCTTAG TGGACAAGCT 
AAAATTATCG AATTGAAGAG CAATGCTACA GAGGAGCAAA AATTAATGTT GCAAGACCTG 
GATACTTTTT ACGCAGATAG TCCTTGCGAT CAACATTTAC CATTGAACCT GGTTATCAAA 
TCTCTATACG ATAAGGCTGT AAATTGGAAC TCTACATTTG CGATATTTGC AAAAAGCTAT 
TTGCCTTCTG AAACTGATAT TAGTCGTATC ACGGCCAAGC AGCTGAAAAA ACTGTTTTAC 
GATTCTTCAA GAAAAGACAG CGAAGACAAA AAATCACTTC ATGATGAAAA GGCGAAGACG 
AGGAAACCAG AAAAGAACGA ATTACCGTTA GAGGGATTGA AAGATGTCGA AAAGCCGAAA 
ATTGATAGCA AGAATACGAC AGAGAATCGT GACAGAACGA ACGAACCACA GAATGCCGTG 
ACGATAACCA CTTTTAAAGA CGACACTCCA ATTATTCCAA CAAGTGGTAC TAGTCACTTG 
ACAGTTACAC CTTCTGCATC GTCGGTTTCT TCTTCTTTGA CCCCTCAAAC CGAAGAACGT 
CCGCCAATAA GCAGAAGTGG AACAGGTATA TCTATGACTC ATGATAAAAG CACTAGACCT 
AACATAAGAA AAATGAGTAG TGATAGTAGT TTATGTGGAC TAGCATCACT TGCTAATGAA 
TATAGTAAAA ATAATAAGGT GAGTAAGCTA GCTACATTTT TCGATCAAAT GCATTTTGAT 
GCCCTGTCGA AAGAATTTGA ACTGGAACGT GAAAGGGAAA GATTACAACT TAATAAGGAT 
AAATACCAAG CCATACGATT ACAAACATCC ACACCAATTG TGGAAATTTA TAAAAATGTC 
AAAGACGCAG TTGATGAACC GCTTCACTCT AGAAGTTCAG GCAATAATTT GTCATCTGCG 
AATGTTAAGA CTTTGGAGGC ACCTGTGGGA GAACATTCCC GGGCCAATAA TTGTAATCCT 
CCTAATTTAG ATCAAAACTT GGAAACTGAA TTAGAAAATT CCATTAGCCA ATGGGGAGAA 
AACATTTTGA ACCCCTCTGG AAAGACCACC GCAAGTACTC ATTTAAATTC TAAGCCAGTA 
GTAAAGGAAA CTTCAGAAAA TCCAAAGTCA ATAGTTAGAG AATCAGATAA TTCAAAGTCG 
GAGCCGCTTC CACCTGTAAT AACAACAACA ACTGTCAATA AGGTAGAATC CACTCCACAA 
CCTGAAAAAT CATTATTGAT GAAAACCTTG AGCAACTTTT GGGCAGATCG ATCTGCGTAT 
TTGTGGAAAC CCCTTGTTTA TCCAACCTGT CCTTCGGAAC ACATTTTTAC TGATAGTGAC 
GTTATAATCC GCGAAGATGA ACCAAGTTCC TTGATCGCAT TTTGCTTAAG TACTTCTGAC 
TATAGAAATA AAATGATGAA CTTGAATGTC CAGCAACAAC AGCAGCAGCA AACAGCAGAA 
GCTGCTCCTG CCAAAACAGG AGGAAACAGT GGAGGAACTA CGCAAACCGG GGATCCTTCT 
GTTAACATTT CTCCCTCAGT TTCTACAACT TCTCATAATA AGGGGAGGGA CTCAGAAATT 
TCATCCTTAG TAACCACCAA AGAGGGCTTG TTGAACACCC CTCCCATTGA AGGCGCCCGA 
GATAGGACCC CACAAGAATC ACAAACGCAT TCCCAAGCAA ATTTAGATAC TCTTCAAGAA 
TTAGAGAAAA TTATGACAAA AAAAACAGCC ACACACTTGA GATATCAATT TGAGGAGGGT 
TTAACGGTAA TGTCATGTAA AATTTTCTTC ACAGAACATT TTGACGTGTT TAGAAAGATT 
TGTGATTGTC AAGAAAACTT CATTCAAAGT TTGTCAAGAT GTGTTAAATG GGATTCCAAT 
GGGGGGAAAA GTGGGAGCGG GTTTTTAAAG ACTTTGGATG ATAGATTTAT TATAAAGGAG 
CTGTCACACG CAGAACTGGA AGCCTTCATA AAATTTGCAC CAAGTTATTT TGAATACATG 
GCACAAGCAA TGTTCCATGA TTTGCCCACG ACTTTGGCTA AAGTATTTGG GTTTTATCAA 
ATTCAAGTAA AAAGCTCTAT TTCAAGTTCC AAAAGTTATA AAATGGACGT TATCATTATG 
GAGAACTTAT TTTATGAGAA GAAAACAACA AGAATATTTG ATTTGAAGGG TTCCATGAGA 
AATAGACATG TCGAACAAAC AGGTAAAGCT AATGAAGTGT TGTTGGATGA AAATATGGTA 
GAGTATATTT ACGAATCACC TATTCATGTT CGCGAATATG ACAAGAAACT TTTAAGAGCT 
TCTGTTTGGA ACGACACATT ATTTTTGGCC AAAATGAATG TGATGGATTA TTCCTTAGTC 
ATTGGTATTG ACAATGAAGG GTATACTTTG ACAGTAGGAA TCATTGATTT CATTCGAACG 
TTTACATGGG ACAAAAAACT AGAAAGTTGG GTCAAAGAGA AAGGCCTAGT AGGGGGGGCC 
AGTGTGATCA AGCAGCCTAC CGTTGTTACA CCAAGACAAT ATAAAAAACG TTTTAGGGAA 
GCAATGGAGA GGTATATTTT GATGGTTCCT GATCCGTGGT ATAGGGAAGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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FAB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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