NECAME_08644 cDNA ORF clone, Necator americanus

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAME_08644
Entrez Gene ID	25348673
Full Name	ribosomal protein L7Ae
Gene Type	protein-coding
Organism	Necator americanus

mRNA and Protein(s)

mRNA	Protein	Name
XM_013448016.1	XP_013303470.1	ribosomal protein L7Ae

Pathways from BioSystems
Gene Ontology

The following NECAME_08644 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAME_08644 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe131505	XM_013448016.1 Latest version!	Necator americanus ribosomal protein L7Ae mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAME_08644 ( XM_013448016.1 ) cDNA ORF clone, Necator americanus -> XP_013303470.1 Necator americanus ribosomal protein L7Ae mRNA.

Price & Availability↑

CloneID	ONe131505
Clone ID Related Accession (Same CDS sequence)	XM_013448016.1
Accession Version	XM_013448016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Necator americanus
Product	ribosomal protein L7Ae
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_013561134). COMPLETENESS: incomplete on both ends.

ATGGCTCCCG CTGCTAAGAA GCCTCAGGTG AAGAAAAATG CTGAGAACAT TAACACTCGA 
TTATCTATGG TTATGAAAAC TGGAAAGTAC GTCTTGGGCT ACAAGCAGAC ATTGAAGGCT 
CTCCGTAACG GAAAGGCCAA GTTGGTCATC ATTGCTAACA ACACTCCTCC TCTCAGGAAG 
TCTGAGATCG AGTACTACGC CATGTTGGCT AAAACCGGTG TCCATCACTA CAATGGAAAC 
AACATTGAGC TGGGAACCGC ATGCGGTAAA CTGTACCACG TATGCACGCT CTCTGTTACT 
GACGCTGGAG ACTCAGACAT TATCCGCAGC ATGCCTTCCG AGACGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013303470.1
CDS	1..351
Translation	MAPAAKKPQVKKNAENINTRLSMVMKTGKYVLGYKQTLKALRNGKAKLVIIANNTPPLRKSEIEYYAMLAKTGVHHYNGNNIELGTACGKLYHVCTLSVTDAGDSDIIRSMPSETA

Target ORF information:

RefSeq Version	XM_013448016.1
Organism	Necator americanus
Definition	Necator americanus ribosomal protein L7Ae mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013448016.1

ORF Insert Sequence:

ATGGCTCCCG CTGCTAAGAA GCCTCAGGTG AAGAAAAATG CTGAGAACAT TAACACTCGA 
TTATCTATGG TTATGAAAAC TGGAAAGTAC GTCTTGGGCT ACAAGCAGAC ATTGAAGGCT 
CTCCGTAACG GAAAGGCCAA GTTGGTCATC ATTGCTAACA ACACTCCTCC TCTCAGGAAG 
TCTGAGATCG AGTACTACGC CATGTTGGCT AAAACCGGTG TCCATCACTA CAATGGAAAC 
AACATTGAGC TGGGAACCGC ATGCGGTAAA CTGTACCACG TATGCACGCT CTCTGTTACT 
GACGCTGGAG ACTCAGACAT TATCCGCAGC ATGCCTTCCG AGACGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.