SPAC20G4.08 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC20G4.08
Entrez Gene ID	5802967
Full Name	conserved fungal protein
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713061.2	XP_001713113.1	conserved fungal protein
NM_001356184.1	NP_001342843.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC20G4.08 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC20G4.08 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102087	NM_001356184.1 Latest version!	Schizosaccharomyces pombe uncharacterized protein (SPAC20G4.08), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSj04991	XM_001713061.2 Latest version!	Schizosaccharomyces pombe 972h- conserved fungal protein (SPAC20G4.08), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC20G4.08 ( NM_001356184.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342843.1 Schizosaccharomyces pombe uncharacterized protein (SPAC20G4.08), mRNA.

Price & Availability↑

CloneID	OSc102087
Clone ID Related Accession (Same CDS sequence)	NM_001356184.1
Accession Version	NM_001356184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-04
Organism	Schizosaccharomyces pombe(fission yeast)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGAATGAGC AAGATCTATT GAATTCTCTT AGAAGAGATT TAAATTTGCC TAATCTTGGA 
AAATCGCATG ATGGATCTGA AGCTGTAGAA TCTACATTCC CAGAGAAAAA AGAATCTTCC 
TTGTCTGCTC AGCAACCTCA TGTCGATGAT CAACGCTCCA GTCTCTTAAG CTTATTGAAT 
GCGGGACTGA ATGCATCAAA CCAGTCACCA TCAAATTCAG GACCAAAATA TTATGCATCC 
CACTCATCTA GTACTGACGC ACTTCTTCAG GCTTTTCGTG ATGGTGCGAA ACCTTCTGGG 
ACTGCCTCTG GCGCAGATGT TAAACGAAGT GATTCTGAGT CTACTGAGGC GACATCTAAT 
GAAAGGCCTT TTAATCCCGT ATCGGCAGCT AATCTAGAAA GGTTACTGAT GTCTTCTACT 
GGGCCACAAA CTCCTATTAA TGGTGAATTA AAATCTAATG ACTCTCAAGA CACGGCTTTC 
CAGTCTTCAA GGAATATGCC ATCTGATACC TCGGTAGCTT CTCCTGATTA CTCGCATTCT 
CAATCAAGCT CGCCTATTGC CAATTACCAA GAGTCGGGTA ACTCGGAAGA ACCGCACAAA 
GCAGAAGAAC AGCAACAGCT GAGTATTTAT CAACTTGACA ATCCTGGTTC CGGTAATTAT 
GTTTGGGAGA CTGTGATTTC TCCCGACAAA TTTGAAACGT CGACATTCGC CAAATGCGAA 
CGAAATGACA TTGCTATTAT AAATCGTGAG CTCGACGCTC AAGATAATCA ACTAATTCAC 
ACCAATGAAG ATTTTATTGC TTATGCTGTT CATCGCGAGC CGATTATTCG AGTCATCGAA 
ATTTCAACTG GAAAGTCTTT TCTTCTCCAT AACAACTCAC CTAATAAGTT TGTATCTGTA 
GCCTGGGGAA ATGATTCGGT TATTAAGAAT AGACTTATGG CTATTGATAC TACAGGACAA 
GTGTTGATCT TTGCTGTTGA TATTGCTACT TCTACTTCTG AAATCATTTT TCAACTATCC 
GGTGCTCAGT CACTGTCAGA TCCTATTAAG TCGAGGTTCC ATTGGTACCC AAAGTCTTCA 
ACTCGTTTTG CTGTTGCTTT AAGTAAGCAC ATTATCTTTT TCGATTTGGA CTTATTAAAC 
AATATTTCTT TTCCCATACC TAGATCAATT AATGCTATCC AACAGCTACC TTGCTTTCTC 
ATTGATACCG GTATTAGTGC AAAGGAATAC GACTTTTCTT ATGATGGGAC AGTATTTGCT 
ACCGTGGACA AGGACGCTTT AATAAAAATA TATACAGTTC CTACTACTTT CCCTTCAACA 
CCTGACAAAC GACCTGTTCC TTCTGAGGTT TCTCCTATTG CTATTTTTAC GACTCGTATG 
GAGCGTGGTC CCTCTAAAAA TTATGAAAAG CCTATTAACT TACGTTTTAT TTCTACTCCT 
GGTACTAACA ATAGTAGATA TTTGGTTATT GTATACGTAA TGAATCAGTT GATAACACTT 
TTTGATCTTT ATTCAAAAAG GAATATACAG ACTTTCCGAT TTAATAATCG TCCGACGGCT 
GCAACGACTA CCTCATTTTC TCAATTTTCG GTGGACAATG AGCGCAGTAC CTTACTGGTA 
GGAAATCCTC CATCAAATAG TATTTATTTT TTTCTCTTCG CAAAAGACGA GACGGTCTCC 
GAACAAGCAC CAATCTATAA TTCAACCTAT GAATTAATAT TGGCATCATT AAACACATCT 
GAACCCGTTC CTGCGGATGC AAAGTTTTCA GTAATTGTAG CTAAAAAATT TGAGAAAGCA 
GCATGTATTA GCTTTACAGC ATGCAAAATT TTGGAATCTG AAGATAAGTA TTGCATTGTT 
GTAAGCAATA CAGATGGTTA TGAATATTAT TCTATCCCGA CTTCAATATT AGATAAGACT 
GGCAAAACAG TTAGATCGCT CGAGAGCGTC CAGAATTATG ATGCAGACAT TGGTGGCACA 
ATCGATTTAA CTGAGAGACA TAGCACTGCT TCTCCTTCAA CTGTTAATTC TGGATTCAGT 
ACTCCCCGTT CACAAGCTAC AGGATTTTCT AAGAAAAAGA AGGACAAAGG CGAGAGATTT 
GAAACTAAGG ACAAATCATC ATCTGTTTTA TCACCTTCTT CATATAGTGC TTCAACTTTT 
GATGCGATAC CTATGGATAG TATTGTCTCT AATATCCTTG CTTCCTTAGA AAAAAGTGTC 
CACAAAAATT ATGAAAGCCT AAGATCTCAA CTTTTGGAAT ATAAGGCTGC AAACGAAAAA 
CATACGGAAG CTATTTTATC AGTCGTCTCC TCAACCTTAA CTGAAAACAC TGGTAAAATT 
CTAGAGTCGG TGGTTGAAAA ATCGATGCAA GTGGCATTAA AGGAAGAAAT TGCGAATTCC 
GTTAGAAATG CTCTCAAAAA TAACTTGGAA AAAATTGAAT CTTTTCTTGA GAATAGCATT 
GCAGAGCTGC AAAATTCTGT TCGAGAGGAT TTTGATAAGC AGACTTCATC TCTAGCTCAA 
CTGAGGTATA GTATTCAGAA TGTTGCACAT GCCCAGAAGG AATCTGAGGT AAAGTATAAC 
GAATTGAATG AACAAGTTAA AACGCTTGAA GGCTATGTTG AAACAGTGCT TGAAAAGTTC 
AATGATTTGA AGATTGAGAA TAAAGTACCA GAGACAGCAC CTGATGTAGT ACCTTCATCA 
TATCCACCTG CTGCGGAAAG TAACGTTTCC GTGTCTAGCG ATACGTCTAC AAAGGACGTG 
GAAAAGCAGG AGCCTTCATC TGCTGAACAG CCTGCTCAGG GAATTGCTGA AAGTCTGAGA 
AGGCTAAAAG AATATGTTAA AGCTGGTTCT GTGAAAGAAT GTGTTGCTGA GTGGTGTAAC 
ATGCCCTCTG TTGCGGGCTT TGACGTTTTG TCTGAAATTT CTTACGATAG AATGTTAGAA 
AATTGTAGCA ATTTATTGCT TCTTACTTTT ATTTATCATA TTTCTCTCCT TGATTCTGTT 
GATGACGATC GCTTATCGAA GCGTATGGAA TACATTTCTA GAATTTGTTT GAACATTGAT 
GTTAATGACC CAAAGGTGGA AACGGTAGTT CACCCTGTTC TTACTTTAAC TCGCGAAGCT 
TTGTTAAGGC AGTCTGAATT TTTCTCGCCT ATTTTTAAAC GCCGTTTAGT TGTTCTTTTG 
CGTGCTCTTG ATGGCAAAAT ATCTGAAATA TCCGTTGCAT CCAGTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342843.1
CDS	167..3397
Translation	MNEQDLLNSLRRDLNLPNLGKSHDGSEAVESTFPEKKESSLSAQQPHVDDQRSSLLSLLNAGLNASNQSPSNSGPKYYASHSSSTDALLQAFRDGAKPSGTASGADVKRSDSESTEATSNERPFNPVSAANLERLLMSSTGPQTPINGELKSNDSQDTAFQSSRNMPSDTSVASPDYSHSQSSSPIANYQESGNSEEPHKAEEQQQLSIYQLDNPGSGNYVWETVISPDKFETSTFAKCERNDIAIINRELDAQDNQLIHTNEDFIAYAVHREPIIRVIEISTGKSFLLHNNSPNKFVSVAWGNDSVIKNRLMAIDTTGQVLIFAVDIATSTSEIIFQLSGAQSLSDPIKSRFHWYPKSSTRFAVALSKHIIFFDLDLLNNISFPIPRSINAIQQLPCFLIDTGISAKEYDFSYDGTVFATVDKDALIKIYTVPTTFPSTPDKRPVPSEVSPIAIFTTRMERGPSKNYEKPINLRFISTPGTNNSRYLVIVYVMNQLITLFDLYSKRNIQTFRFNNRPTAATTTSFSQFSVDNERSTLLVGNPPSNSIYFFLFAKDETVSEQAPIYNSTYELILASLNTSEPVPADAKFSVIVAKKFEKAACISFTACKILESEDKYCIVVSNTDGYEYYSIPTSILDKTGKTVRSLESVQNYDADIGGTIDLTERHSTASPSTVNSGFSTPRSQATGFSKKKKDKGERFETKDKSSSVLSPSSYSASTFDAIPMDSIVSNILASLEKSVHKNYESLRSQLLEYKAANEKHTEAILSVVSSTLTENTGKILESVVEKSMQVALKEEIANSVRNALKNNLEKIESFLENSIAELQNSVREDFDKQTSSLAQLRYSIQNVAHAQKESEVKYNELNEQVKTLEGYVETVLEKFNDLKIENKVPETAPDVVPSSYPPAAESNVSVSSDTSTKDVEKQEPSSAEQPAQGIAESLRRLKEYVKAGSVKECVAEWCNMPSVAGFDVLSEISYDRMLENCSNLLLLTFIYHISLLDSVDDDRLSKRMEYISRICLNIDVNDPKVETVVHPVLTLTREALLRQSEFFSPIFKRRLVVLLRALDGKISEISVASSN

Target ORF information:

RefSeq Version	NM_001356184.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe uncharacterized protein (SPAC20G4.08), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356184.1

ORF Insert Sequence:

ATGAATGAGC AAGATCTATT GAATTCTCTT AGAAGAGATT TAAATTTGCC TAATCTTGGA 
AAATCGCATG ATGGATCTGA AGCTGTAGAA TCTACATTCC CAGAGAAAAA AGAATCTTCC 
TTGTCTGCTC AGCAACCTCA TGTCGATGAT CAACGCTCCA GTCTCTTAAG CTTATTGAAT 
GCGGGACTGA ATGCATCAAA CCAGTCACCA TCAAATTCAG GACCAAAATA TTATGCATCC 
CACTCATCTA GTACTGACGC ACTTCTTCAG GCTTTTCGTG ATGGTGCGAA ACCTTCTGGG 
ACTGCCTCTG GCGCAGATGT TAAACGAAGT GATTCTGAGT CTACTGAGGC GACATCTAAT 
GAAAGGCCTT TTAATCCCGT ATCGGCAGCT AATCTAGAAA GGTTACTGAT GTCTTCTACT 
GGGCCACAAA CTCCTATTAA TGGTGAATTA AAATCTAATG ACTCTCAAGA CACGGCTTTC 
CAGTCTTCAA GGAATATGCC ATCTGATACC TCGGTAGCTT CTCCTGATTA CTCGCATTCT 
CAATCAAGCT CGCCTATTGC CAATTACCAA GAGTCGGGTA ACTCGGAAGA ACCGCACAAA 
GCAGAAGAAC AGCAACAGCT GAGTATTTAT CAACTTGACA ATCCTGGTTC CGGTAATTAT 
GTTTGGGAGA CTGTGATTTC TCCCGACAAA TTTGAAACGT CGACATTCGC CAAATGCGAA 
CGAAATGACA TTGCTATTAT AAATCGTGAG CTCGACGCTC AAGATAATCA ACTAATTCAC 
ACCAATGAAG ATTTTATTGC TTATGCTGTT CATCGCGAGC CGATTATTCG AGTCATCGAA 
ATTTCAACTG GAAAGTCTTT TCTTCTCCAT AACAACTCAC CTAATAAGTT TGTATCTGTA 
GCCTGGGGAA ATGATTCGGT TATTAAGAAT AGACTTATGG CTATTGATAC TACAGGACAA 
GTGTTGATCT TTGCTGTTGA TATTGCTACT TCTACTTCTG AAATCATTTT TCAACTATCC 
GGTGCTCAGT CACTGTCAGA TCCTATTAAG TCGAGGTTCC ATTGGTACCC AAAGTCTTCA 
ACTCGTTTTG CTGTTGCTTT AAGTAAGCAC ATTATCTTTT TCGATTTGGA CTTATTAAAC 
AATATTTCTT TTCCCATACC TAGATCAATT AATGCTATCC AACAGCTACC TTGCTTTCTC 
ATTGATACCG GTATTAGTGC AAAGGAATAC GACTTTTCTT ATGATGGGAC AGTATTTGCT 
ACCGTGGACA AGGACGCTTT AATAAAAATA TATACAGTTC CTACTACTTT CCCTTCAACA 
CCTGACAAAC GACCTGTTCC TTCTGAGGTT TCTCCTATTG CTATTTTTAC GACTCGTATG 
GAGCGTGGTC CCTCTAAAAA TTATGAAAAG CCTATTAACT TACGTTTTAT TTCTACTCCT 
GGTACTAACA ATAGTAGATA TTTGGTTATT GTATACGTAA TGAATCAGTT GATAACACTT 
TTTGATCTTT ATTCAAAAAG GAATATACAG ACTTTCCGAT TTAATAATCG TCCGACGGCT 
GCAACGACTA CCTCATTTTC TCAATTTTCG GTGGACAATG AGCGCAGTAC CTTACTGGTA 
GGAAATCCTC CATCAAATAG TATTTATTTT TTTCTCTTCG CAAAAGACGA GACGGTCTCC 
GAACAAGCAC CAATCTATAA TTCAACCTAT GAATTAATAT TGGCATCATT AAACACATCT 
GAACCCGTTC CTGCGGATGC AAAGTTTTCA GTAATTGTAG CTAAAAAATT TGAGAAAGCA 
GCATGTATTA GCTTTACAGC ATGCAAAATT TTGGAATCTG AAGATAAGTA TTGCATTGTT 
GTAAGCAATA CAGATGGTTA TGAATATTAT TCTATCCCGA CTTCAATATT AGATAAGACT 
GGCAAAACAG TTAGATCGCT CGAGAGCGTC CAGAATTATG ATGCAGACAT TGGTGGCACA 
ATCGATTTAA CTGAGAGACA TAGCACTGCT TCTCCTTCAA CTGTTAATTC TGGATTCAGT 
ACTCCCCGTT CACAAGCTAC AGGATTTTCT AAGAAAAAGA AGGACAAAGG CGAGAGATTT 
GAAACTAAGG ACAAATCATC ATCTGTTTTA TCACCTTCTT CATATAGTGC TTCAACTTTT 
GATGCGATAC CTATGGATAG TATTGTCTCT AATATCCTTG CTTCCTTAGA AAAAAGTGTC 
CACAAAAATT ATGAAAGCCT AAGATCTCAA CTTTTGGAAT ATAAGGCTGC AAACGAAAAA 
CATACGGAAG CTATTTTATC AGTCGTCTCC TCAACCTTAA CTGAAAACAC TGGTAAAATT 
CTAGAGTCGG TGGTTGAAAA ATCGATGCAA GTGGCATTAA AGGAAGAAAT TGCGAATTCC 
GTTAGAAATG CTCTCAAAAA TAACTTGGAA AAAATTGAAT CTTTTCTTGA GAATAGCATT 
GCAGAGCTGC AAAATTCTGT TCGAGAGGAT TTTGATAAGC AGACTTCATC TCTAGCTCAA 
CTGAGGTATA GTATTCAGAA TGTTGCACAT GCCCAGAAGG AATCTGAGGT AAAGTATAAC 
GAATTGAATG AACAAGTTAA AACGCTTGAA GGCTATGTTG AAACAGTGCT TGAAAAGTTC 
AATGATTTGA AGATTGAGAA TAAAGTACCA GAGACAGCAC CTGATGTAGT ACCTTCATCA 
TATCCACCTG CTGCGGAAAG TAACGTTTCC GTGTCTAGCG ATACGTCTAC AAAGGACGTG 
GAAAAGCAGG AGCCTTCATC TGCTGAACAG CCTGCTCAGG GAATTGCTGA AAGTCTGAGA 
AGGCTAAAAG AATATGTTAA AGCTGGTTCT GTGAAAGAAT GTGTTGCTGA GTGGTGTAAC 
ATGCCCTCTG TTGCGGGCTT TGACGTTTTG TCTGAAATTT CTTACGATAG AATGTTAGAA 
AATTGTAGCA ATTTATTGCT TCTTACTTTT ATTTATCATA TTTCTCTCCT TGATTCTGTT 
GATGACGATC GCTTATCGAA GCGTATGGAA TACATTTCTA GAATTTGTTT GAACATTGAT 
GTTAATGACC CAAAGGTGGA AACGGTAGTT CACCCTGTTC TTACTTTAAC TCGCGAAGCT 
TTGTTAAGGC AGTCTGAATT TTTCTCGCCT ATTTTTAAAC GCCGTTTAGT TGTTCTTTTG 
CGTGCTCTTG ATGGCAAAAT ATCTGAAATA TCCGTTGCAT CCAGTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAC20G4.08 ( XM_001713061.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713113.1 Schizosaccharomyces pombe 972h- conserved fungal protein (SPAC20G4.08), mRNA.

Price & Availability↑

CloneID	OSj04991
Clone ID Related Accession (Same CDS sequence)	XM_001713061.2
Accession Version	XM_001713061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	conserved fungal protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001713061.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAATGAGC AAGATCTATT GAATTCTCTT AGAAGAGATT TAAATTTGCC TAATCTTGGA 
AAATCGCATG ATGGATCTGA AGCTGTAGAA TCTACATTCC CAGAGAAAAA AGAATCTTCC 
TTGTCTGCTC AGCAACCTCA TGTCGATGAT CAACGCTCCA GTCTCTTAAG CTTATTGAAT 
GCGGGACTGA ATGCATCAAA CCAGTCACCA TCAAATTCAG GACCAAAATA TTATGCATCC 
CACTCATCTA GTACTGACGC ACTTCTTCAG GCTTTTCGTG ATGGTGCGAA ACCTTCTGGG 
ACTGCCTCTG GCGCAGATGT TAAACGAAGT GATTCTGAGT CTACTGAGGC GACATCTAAT 
GAAAGGCCTT TTAATCCCGT ATCGGCAGCT AATCTAGAAA GGTTACTGAT GTCTTCTACT 
GGGCCACAAA CTCCTATTAA TGGTGAATTA AAATCTAATG ACTCTCAAGA CACGGCTTTC 
CAGTCTTCAA GGAATATGCC ATCTGATACC TCGGTAGCTT CTCCTGATTA CTCGCATTCT 
CAATCAAGCT CGCCTATTGC CAATTACCAA GAGTCGGGTA ACTCGGAAGA ACCGCACAAA 
GCAGAAGAAC AGCAACAGCT GAGTATTTAT CAACTTGACA ATCCTGGTTC CGGTAATTAT 
GTTTGGGAGA CTGTGATTTC TCCCGACAAA TTTGAAACGT CGACATTCGC CAAATGCGAA 
CGAAATGACA TTGCTATTAT AAATCGTGAG CTCGACGCTC AAGATAATCA ACTAATTCAC 
ACCAATGAAG ATTTTATTGC TTATGCTGTT CATCGCGAGC CGATTATTCG AGTCATCGAA 
ATTTCAACTG GAAAGTCTTT TCTTCTCCAT AACAACTCAC CTAATAAGTT TGTATCTGTA 
GCCTGGGGAA ATGATTCGGT TATTAAGAAT AGACTTATGG CTATTGATAC TACAGGACAA 
GTGTTGATCT TTGCTGTTGA TATTGCTACT TCTACTTCTG AAATCATTTT TCAACTATCC 
GGTGCTCAGT CACTGTCAGA TCCTATTAAG TCGAGGTTCC ATTGGTACCC AAAGTCTTCA 
ACTCGTTTTG CTGTTGCTTT AAGTAAGCAC ATTATCTTTT TCGATTTGGA CTTATTAAAC 
AATATTTCTT TTCCCATACC TAGATCAATT AATGCTATCC AACAGCTACC TTGCTTTCTC 
ATTGATACCG GTATTAGTGC AAAGGAATAC GACTTTTCTT ATGATGGGAC AGTATTTGCT 
ACCGTGGACA AGGACGCTTT AATAAAAATA TATACAGTTC CTACTACTTT CCCTTCAACA 
CCTGACAAAC GACCTGTTCC TTCTGAGGTT TCTCCTATTG CTATTTTTAC GACTCGTATG 
GAGCGTGGTC CCTCTAAAAA TTATGAAAAG CCTATTAACT TACGTTTTAT TTCTACTCCT 
GGTACTAACA ATAGTAGATA TTTGGTTATT GTATACGTAA TGAATCAGTT GATAACACTT 
TTTGATCTTT ATTCAAAAAG GAATATACAG ACTTTCCGAT TTAATAATCG TCCGACGGCT 
GCAACGACTA CCTCATTTTC TCAATTTTCG GTGGACAATG AGCGCAGTAC CTTACTGGTA 
GGAAATCCTC CATCAAATAG TATTTATTTT TTTCTCTTCG CAAAAGACGA GACGGTCTCC 
GAACAAGCAC CAATCTATAA TTCAACCTAT GAATTAATAT TGGCATCATT AAACACATCT 
GAACCCGTTC CTGCGGATGC AAAGTTTTCA GTAATTGTAG CTAAAAAATT TGAGAAAGCA 
GCATGTATTA GCTTTACAGC ATGCAAAATT TTGGAATCTG AAGATAAGTA TTGCATTGTT 
GTAAGCAATA CAGATGGTTA TGAATATTAT TCTATCCCGA CTTCAATATT AGATAAGACT 
GGCAAAACAG TTAGATCGCT CGAGAGCGTC CAGAATTATG ATGCAGACAT TGGTGGCACA 
ATCGATTTAA CTGAGAGACA TAGCACTGCT TCTCCTTCAA CTGTTAATTC TGGATTCAGT 
ACTCCCCGTT CACAAGCTAC AGGATTTTCT AAGAAAAAGA AGGACAAAGG CGAGAGATTT 
GAAACTAAGG ACAAATCATC ATCTGTTTTA TCACCTTCTT CATATAGTGC TTCAACTTTT 
GATGCGATAC CTATGGATAG TATTGTCTCT AATATCCTTG CTTCCTTAGA AAAAAGTGTC 
CACAAAAATT ATGAAAGCCT AAGATCTCAA CTTTTGGAAT ATAAGGCTGC AAACGAAAAA 
CATACGGAAG CTATTTTATC AGTCGTCTCC TCAACCTTAA CTGAAAACAC TGGTAAAATT 
CTAGAGTCGG TGGTTGAAAA ATCGATGCAA GTGGCATTAA AGGAAGAAAT TGCGAATTCC 
GTTAGAAATG CTCTCAAAAA TAACTTGGAA AAAATTGAAT CTTTTCTTGA GAATAGCATT 
GCAGAGCTGC AAAATTCTGT TCGAGAGGAT TTTGATAAGC AGACTTCATC TCTAGCTCAA 
CTGAGGTATA GTATTCAGAA TGTTGCACAT GCCCAGAAGG AATCTGAGGT AAAGTATAAC 
GAATTGAATG AACAAGTTAA AACGCTTGAA GGCTATGTTG AAACAGTGCT TGAAAAGTTC 
AATGATTTGA AGATTGAGAA TAAAGTACCA GAGACAGCAC CTGATGTAGT ACCTTCATCA 
TATCCACCTG CTGCGGAAAG TAACGTTTCC GTGTCTAGCG ATACGTCTAC AAAGGACGTG 
GAAAAGCAGG AGCCTTCATC TGCTGAACAG CCTGCTCAGG GAATTGCTGA AAGTCTGAGA 
AGGCTAAAAG AATATGTTAA AGCTGGTTCT GTGAAAGAAT GTGTTGCTGA GTGGTGTAAC 
ATGCCCTCTG TTGCGGGCTT TGACGTTTTG TCTGAAATTT CTTACGATAG AATGTTAGAA 
AATTGTAGCA ATTTATTGCT TCTTACTTTT ATTTATCATA TTTCTCTCCT TGATTCTGTT 
GATGACGATC GCTTATCGAA GCGTATGGAA TACATTTCTA GAATTTGTTT GAACATTGAT 
GTTAATGACC CAAAGGTGGA AACGGTAGTT CACCCTGTTC TTACTTTAAC TCGCGAAGCT 
TTGTTAAGGC AGTCTGAATT TTTCTCGCCT ATTTTTAAAC GCCGTTTAGT TGTTCTTTTG 
CGTGCTCTTG ATGGCAAAAT ATCTGAAATA TCCGTTGCAT CCAGTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713113.1
CDS	167..3397
Translation	MNEQDLLNSLRRDLNLPNLGKSHDGSEAVESTFPEKKESSLSAQQPHVDDQRSSLLSLLNAGLNASNQSPSNSGPKYYASHSSSTDALLQAFRDGAKPSGTASGADVKRSDSESTEATSNERPFNPVSAANLERLLMSSTGPQTPINGELKSNDSQDTAFQSSRNMPSDTSVASPDYSHSQSSSPIANYQESGNSEEPHKAEEQQQLSIYQLDNPGSGNYVWETVISPDKFETSTFAKCERNDIAIINRELDAQDNQLIHTNEDFIAYAVHREPIIRVIEISTGKSFLLHNNSPNKFVSVAWGNDSVIKNRLMAIDTTGQVLIFAVDIATSTSEIIFQLSGAQSLSDPIKSRFHWYPKSSTRFAVALSKHIIFFDLDLLNNISFPIPRSINAIQQLPCFLIDTGISAKEYDFSYDGTVFATVDKDALIKIYTVPTTFPSTPDKRPVPSEVSPIAIFTTRMERGPSKNYEKPINLRFISTPGTNNSRYLVIVYVMNQLITLFDLYSKRNIQTFRFNNRPTAATTTSFSQFSVDNERSTLLVGNPPSNSIYFFLFAKDETVSEQAPIYNSTYELILASLNTSEPVPADAKFSVIVAKKFEKAACISFTACKILESEDKYCIVVSNTDGYEYYSIPTSILDKTGKTVRSLESVQNYDADIGGTIDLTERHSTASPSTVNSGFSTPRSQATGFSKKKKDKGERFETKDKSSSVLSPSSYSASTFDAIPMDSIVSNILASLEKSVHKNYESLRSQLLEYKAANEKHTEAILSVVSSTLTENTGKILESVVEKSMQVALKEEIANSVRNALKNNLEKIESFLENSIAELQNSVREDFDKQTSSLAQLRYSIQNVAHAQKESEVKYNELNEQVKTLEGYVETVLEKFNDLKIENKVPETAPDVVPSSYPPAAESNVSVSSDTSTKDVEKQEPSSAEQPAQGIAESLRRLKEYVKAGSVKECVAEWCNMPSVAGFDVLSEISYDRMLENCSNLLLLTFIYHISLLDSVDDDRLSKRMEYISRICLNIDVNDPKVETVVHPVLTLTREALLRQSEFFSPIFKRRLVVLLRALDGKISEISVASSN

Target ORF information:

RefSeq Version	XM_001713061.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- conserved fungal protein (SPAC20G4.08), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713061.2

ORF Insert Sequence:

ATGAATGAGC AAGATCTATT GAATTCTCTT AGAAGAGATT TAAATTTGCC TAATCTTGGA 
AAATCGCATG ATGGATCTGA AGCTGTAGAA TCTACATTCC CAGAGAAAAA AGAATCTTCC 
TTGTCTGCTC AGCAACCTCA TGTCGATGAT CAACGCTCCA GTCTCTTAAG CTTATTGAAT 
GCGGGACTGA ATGCATCAAA CCAGTCACCA TCAAATTCAG GACCAAAATA TTATGCATCC 
CACTCATCTA GTACTGACGC ACTTCTTCAG GCTTTTCGTG ATGGTGCGAA ACCTTCTGGG 
ACTGCCTCTG GCGCAGATGT TAAACGAAGT GATTCTGAGT CTACTGAGGC GACATCTAAT 
GAAAGGCCTT TTAATCCCGT ATCGGCAGCT AATCTAGAAA GGTTACTGAT GTCTTCTACT 
GGGCCACAAA CTCCTATTAA TGGTGAATTA AAATCTAATG ACTCTCAAGA CACGGCTTTC 
CAGTCTTCAA GGAATATGCC ATCTGATACC TCGGTAGCTT CTCCTGATTA CTCGCATTCT 
CAATCAAGCT CGCCTATTGC CAATTACCAA GAGTCGGGTA ACTCGGAAGA ACCGCACAAA 
GCAGAAGAAC AGCAACAGCT GAGTATTTAT CAACTTGACA ATCCTGGTTC CGGTAATTAT 
GTTTGGGAGA CTGTGATTTC TCCCGACAAA TTTGAAACGT CGACATTCGC CAAATGCGAA 
CGAAATGACA TTGCTATTAT AAATCGTGAG CTCGACGCTC AAGATAATCA ACTAATTCAC 
ACCAATGAAG ATTTTATTGC TTATGCTGTT CATCGCGAGC CGATTATTCG AGTCATCGAA 
ATTTCAACTG GAAAGTCTTT TCTTCTCCAT AACAACTCAC CTAATAAGTT TGTATCTGTA 
GCCTGGGGAA ATGATTCGGT TATTAAGAAT AGACTTATGG CTATTGATAC TACAGGACAA 
GTGTTGATCT TTGCTGTTGA TATTGCTACT TCTACTTCTG AAATCATTTT TCAACTATCC 
GGTGCTCAGT CACTGTCAGA TCCTATTAAG TCGAGGTTCC ATTGGTACCC AAAGTCTTCA 
ACTCGTTTTG CTGTTGCTTT AAGTAAGCAC ATTATCTTTT TCGATTTGGA CTTATTAAAC 
AATATTTCTT TTCCCATACC TAGATCAATT AATGCTATCC AACAGCTACC TTGCTTTCTC 
ATTGATACCG GTATTAGTGC AAAGGAATAC GACTTTTCTT ATGATGGGAC AGTATTTGCT 
ACCGTGGACA AGGACGCTTT AATAAAAATA TATACAGTTC CTACTACTTT CCCTTCAACA 
CCTGACAAAC GACCTGTTCC TTCTGAGGTT TCTCCTATTG CTATTTTTAC GACTCGTATG 
GAGCGTGGTC CCTCTAAAAA TTATGAAAAG CCTATTAACT TACGTTTTAT TTCTACTCCT 
GGTACTAACA ATAGTAGATA TTTGGTTATT GTATACGTAA TGAATCAGTT GATAACACTT 
TTTGATCTTT ATTCAAAAAG GAATATACAG ACTTTCCGAT TTAATAATCG TCCGACGGCT 
GCAACGACTA CCTCATTTTC TCAATTTTCG GTGGACAATG AGCGCAGTAC CTTACTGGTA 
GGAAATCCTC CATCAAATAG TATTTATTTT TTTCTCTTCG CAAAAGACGA GACGGTCTCC 
GAACAAGCAC CAATCTATAA TTCAACCTAT GAATTAATAT TGGCATCATT AAACACATCT 
GAACCCGTTC CTGCGGATGC AAAGTTTTCA GTAATTGTAG CTAAAAAATT TGAGAAAGCA 
GCATGTATTA GCTTTACAGC ATGCAAAATT TTGGAATCTG AAGATAAGTA TTGCATTGTT 
GTAAGCAATA CAGATGGTTA TGAATATTAT TCTATCCCGA CTTCAATATT AGATAAGACT 
GGCAAAACAG TTAGATCGCT CGAGAGCGTC CAGAATTATG ATGCAGACAT TGGTGGCACA 
ATCGATTTAA CTGAGAGACA TAGCACTGCT TCTCCTTCAA CTGTTAATTC TGGATTCAGT 
ACTCCCCGTT CACAAGCTAC AGGATTTTCT AAGAAAAAGA AGGACAAAGG CGAGAGATTT 
GAAACTAAGG ACAAATCATC ATCTGTTTTA TCACCTTCTT CATATAGTGC TTCAACTTTT 
GATGCGATAC CTATGGATAG TATTGTCTCT AATATCCTTG CTTCCTTAGA AAAAAGTGTC 
CACAAAAATT ATGAAAGCCT AAGATCTCAA CTTTTGGAAT ATAAGGCTGC AAACGAAAAA 
CATACGGAAG CTATTTTATC AGTCGTCTCC TCAACCTTAA CTGAAAACAC TGGTAAAATT 
CTAGAGTCGG TGGTTGAAAA ATCGATGCAA GTGGCATTAA AGGAAGAAAT TGCGAATTCC 
GTTAGAAATG CTCTCAAAAA TAACTTGGAA AAAATTGAAT CTTTTCTTGA GAATAGCATT 
GCAGAGCTGC AAAATTCTGT TCGAGAGGAT TTTGATAAGC AGACTTCATC TCTAGCTCAA 
CTGAGGTATA GTATTCAGAA TGTTGCACAT GCCCAGAAGG AATCTGAGGT AAAGTATAAC 
GAATTGAATG AACAAGTTAA AACGCTTGAA GGCTATGTTG AAACAGTGCT TGAAAAGTTC 
AATGATTTGA AGATTGAGAA TAAAGTACCA GAGACAGCAC CTGATGTAGT ACCTTCATCA 
TATCCACCTG CTGCGGAAAG TAACGTTTCC GTGTCTAGCG ATACGTCTAC AAAGGACGTG 
GAAAAGCAGG AGCCTTCATC TGCTGAACAG CCTGCTCAGG GAATTGCTGA AAGTCTGAGA 
AGGCTAAAAG AATATGTTAA AGCTGGTTCT GTGAAAGAAT GTGTTGCTGA GTGGTGTAAC 
ATGCCCTCTG TTGCGGGCTT TGACGTTTTG TCTGAAATTT CTTACGATAG AATGTTAGAA 
AATTGTAGCA ATTTATTGCT TCTTACTTTT ATTTATCATA TTTCTCTCCT TGATTCTGTT 
GATGACGATC GCTTATCGAA GCGTATGGAA TACATTTCTA GAATTTGTTT GAACATTGAT 
GTTAATGACC CAAAGGTGGA AACGGTAGTT CACCCTGTTC TTACTTTAAC TCGCGAAGCT 
TTGTTAAGGC AGTCTGAATT TTTCTCGCCT ATTTTTAAAC GCCGTTTAGT TGTTCTTTTG 
CGTGCTCTTG ATGGCAAAAT ATCTGAAATA TCCGTTGCAT CCAGTAATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC20G4.08 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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