sec73 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec73
Entrez Gene ID	3361502
Full Name	guanyl-nucleotide exchange factor Sec73 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713015.2	XP_001713067.1	guanyl-nucleotide exchange factor Sec73 (predicted)
NM_001356066.1	NP_001342851.1	putative guanyl-nucleotide exchange factor Sec73

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sec73 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec73 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101899	NM_001356066.1 Latest version!	Schizosaccharomyces pombe putative guanyl-nucleotide exchange factor Sec73 (sec73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSj04926	XM_001713015.2 Latest version!	Schizosaccharomyces pombe 972h- guanyl-nucleotide exchange factor Sec73 (predicted) (sec73), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec73 ( NM_001356066.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342851.1 Schizosaccharomyces pombe putative guanyl-nucleotide exchange factor Sec73 (sec73), mRNA.

Price & Availability↑

CloneID	OSc101899
Clone ID Related Accession (Same CDS sequence)	NM_001356066.1
Accession Version	NM_001356066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative guanyl-nucleotide exchange factor Sec73
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGACCTTCA GAATTCCTTC CAGCCAGGAG TCTTCAAATA AATCCGAAAA GGATACCACT 
GTAAATTCTC CTAACGCTTC TAGTTTCTCC TCTTTCTTTA AAAAGTCTAG TCAGACATAT 
CTGTCCAAAC GTTCAGAAAA ACTAAATGAG CATAACAGTA AACTAAAGTC AAGCGGATCT 
TTCACAAGTA ATTCACAGAC CGACTTGAGT AATTCGCCCT CTTCTAAGAA AAAAAATATT 
TTTTATCCGA TCGCAAGAGC CAAGCACTCG TTTTACTTCA ACCACAACTG GTCTTCGGAT 
TTGCAGCAAG CAAATTCTCC TGTTGCATCG TATTCAAGTC AAAGCGTTTC AAACGATTCC 
AATTTTCCAA AAGATGTCAC GTCTCCTATT TCGACAGACC TAAGCGGTTC CAATCCTTCT 
TTGAAATCAC CATCTAGTAT CAACTCGGCG AAACTTCGTG CTTCTCGATC ATTTTTTCGA 
TTTCCGAAGT GGTCTAGGAA GTCCTGGAAT CCCGATGTTA ACTCAACTAC TAGTTCTCCT 
TCTGAAGTTG GTTCTCTCGA TAAAGCTGGT GGCCCTCTAA ACCAGCAAAA TGAGAATTTG 
TCAGCGGAAT CTGATAATAC GATTTTACAG AATGGTACTC GGAAAAGTAG TGTCAAAATG 
TCCGACCTGG GGAAACGCAT TGCTTCTTTA CCTGTAGTAA AAAGACCTCC TATCCAGTTT 
TCCATATCTT CTGATTACAT TAAGCCAGTT GATAAAAGCG ATCAGACTTC TAACGTTCCA 
TCACCTTCTT CTTCTGTGGG TTCCCTCCGG TTGTATCGTT CGCCTTCTAT TCCTAATGAT 
GTTGAACAAC CACAGCCACA AAGCTCTCGG TCACGGCCTC GTAGTTCTAC GATTCCTACC 
CAAAGGACTT TAGAAAGATT GAGAGAAAAC CGTTCTAGTA TTTTTAGTTT TCGAACTTTG 
CGCCGGGATG ATGACGAATG CGTAGCTCAG ACTATTGAAA ATTCGGCTTT ATTTTCTGAT 
ATTAAGCCTA ATCCGACCAA GTTTCTTTCT TCTGTTCCGG AGCTCACAGC AGATGATACT 
GCGGAGAGTT ATCTCTCAAA GCTTAGAGAG ACAGTTCCTT ATAGTCGTAT GATCCCTGTT 
CTAGCAGAAA AGGATCATGT AACATTGAAC AAAGCTCTGC ACCTTTGTAT GTCGGGAATG 
GAGTTTGAAA ATCGGCCGTT GGATTTTTGC TTGCGGTTAT TTTTACTTAA ATCTCACCTT 
CCTAAGGAAA CTCAACAGAT TGATCGTGTC ATCAATGCAT TCTCTAAGCG CTATTTTGAA 
TGTAACCCTG GGATGTTCAG TTCCCAAGAT CAGTGTTACA TTCTGGTGTT TTCTTTGATG 
ATGCTCCACA CTGACGTTTT TAATTCAAAT AATAAGCATA AAATGACGAA GCAGGAATTC 
ATAAATCTCT GTGACATAGA TGAATTAGCT CCGGAGATAA TGGAGTATTA TTATGACAAT 
ATTACCTTTA CTCCTTTTGT CAACCTGGAT GATGAGTTGC TTCTCATTGA GAAGGAAAAG 
AACGAAAAGT ACAATTTCAC ATCTCGTAAA AGGTTAGGGC TTTCTCCTTA CACATTTACG 
TTAGAAAATC AATTGAATAT TTCACGGCCT GAAATATCTC TCTCTTATGA TGGAAAATTC 
ATATCTCCAG GTATCAAAGA TATTGCGTCT TTGCTGAACG TTAGAAAGTT GATTTCCAGC 
CCTGCGATAA TTCAGCTTGT TTCTAAAAGA TCTCATCCAA TGGCATTCTC CAATCATTTT 
ATTGGATTAC CAGACAATGC TGACCCGGGA CTAGTTGATT TTAGTATCAG TATGCTGGGC 
ATTTTACCAC GGTGTGAAAA GAAGCGAAGA AGTGTGGGGC ACAATTCATA CAAAGAGCAT 
TTGGTTTTGT TGACCGCTTC TAGAATACTT TTTTTTCGTA ATTTGGTATG GGCTAAAGAT 
CTCATGGCTC AAAAGAAAGC TTATGAAAGG CGTTACAAAA ATTTTACAAC TACCTCGAGC 
AGTTTTGACA CGTTGCAAGG TATAGATACA GAGGCTATGG AATATGAATT TCCAAAAATT 
CCTCCGCTTG TATTTACTCC ACCTCTAGAA GAGTTTTCCC CCGATACCAG TTATTCGCTT 
GTTAATGCGT TTGCCTATTG TGACAGAGAT AAGAAGAATG TACGCAATTG TTCTTTTACC 
GTCATATTTC GGGATGGATC TGAAGAGACT CTTTGTGCTC CAACAGAAGA AAGCATGATA 
GAATGGGTAG CTAAAATTAA CTACATCTCA ACACTTCGTA CAGCTGGTCT GCGAATGCCT 
GTAAGTGATT CTTACCATCC TAAAAGAGAC TTTTCTTGCT ATCCAAAGTA TATGGAGGAA 
GAATTAGCAG CAATTGAAGC TCAAATGAAA CAGGATATGG AAATTGCACG TGCCCGGACA 
ATTAAGACAC GTATTGACCA ACTTGAAACA TCTATTACTG AGTTCAATAA GAAACTCATG 
GTATTACAAA GAAATGCCAA AAATTTATCG ACATGTGCAC CCCTACAGCC GAAAACAAGA 
GTAGTTATTT TACAAGCCCT TAAAAAGGTC GATGCTAAAG CGCAATCATT ATATTTGGAA 
ATCAGAAAAA TGGAGTCACA ATTGCAAGTA TTGCGGTTTG AATCTGATTT AGATAAATGC 
ATTTCTCTTG AAAGTTCTAA TGACATCGAC GACAGACCTT CTTCGACAAG CCCAAGCACC 
CTAAAAAATC CGAGCATCAA CTCAATCAAT CGTCAAACAC ATGATGTTGA AAAAGAATCA 
TCAGATAGAG ATGTACCGCA TCTTTTGGAT CCTGATAATA ATTTCAACTT GAAAGGCAAC 
CCCGAAAACC CCTCATCGAC ATTTTTTACT CCTGAAATAC TTTCACCAAA GGTTTACTCG 
GACAATGTTA AAGATGAGCC TGTCGAAGAT ATTTTTGGTT TTGAAGATAC AAGCGAAAGC 
TCGCCCGGTA AACCCAGCCA ATTGAGGCTT CAAATTGAAG CCTTTGATGA TGAATTAGCT 
GACACAGCTA GCTTAGGTTC CGTGACTACA TGTTTAGATC AATGGCAAAC ATTTTCGGAC 
GATACAGATA GCGGGTTTGC TTTGAACCTG TACTCATCAG AAGATTTTCG CACGGATCCA 
TATAAGGAAC ATGATGAGTT TAGTATGAAT AGCCTATCGA AATGGCATTC TGCTACTTCT 
GATAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342851.1
CDS	780..4028
Translation	MTFRIPSSQESSNKSEKDTTVNSPNASSFSSFFKKSSQTYLSKRSEKLNEHNSKLKSSGSFTSNSQTDLSNSPSSKKKNIFYPIARAKHSFYFNHNWSSDLQQANSPVASYSSQSVSNDSNFPKDVTSPISTDLSGSNPSLKSPSSINSAKLRASRSFFRFPKWSRKSWNPDVNSTTSSPSEVGSLDKAGGPLNQQNENLSAESDNTILQNGTRKSSVKMSDLGKRIASLPVVKRPPIQFSISSDYIKPVDKSDQTSNVPSPSSSVGSLRLYRSPSIPNDVEQPQPQSSRSRPRSSTIPTQRTLERLRENRSSIFSFRTLRRDDDECVAQTIENSALFSDIKPNPTKFLSSVPELTADDTAESYLSKLRETVPYSRMIPVLAEKDHVTLNKALHLCMSGMEFENRPLDFCLRLFLLKSHLPKETQQIDRVINAFSKRYFECNPGMFSSQDQCYILVFSLMMLHTDVFNSNNKHKMTKQEFINLCDIDELAPEIMEYYYDNITFTPFVNLDDELLLIEKEKNEKYNFTSRKRLGLSPYTFTLENQLNISRPEISLSYDGKFISPGIKDIASLLNVRKLISSPAIIQLVSKRSHPMAFSNHFIGLPDNADPGLVDFSISMLGILPRCEKKRRSVGHNSYKEHLVLLTASRILFFRNLVWAKDLMAQKKAYERRYKNFTTTSSSFDTLQGIDTEAMEYEFPKIPPLVFTPPLEEFSPDTSYSLVNAFAYCDRDKKNVRNCSFTVIFRDGSEETLCAPTEESMIEWVAKINYISTLRTAGLRMPVSDSYHPKRDFSCYPKYMEEELAAIEAQMKQDMEIARARTIKTRIDQLETSITEFNKKLMVLQRNAKNLSTCAPLQPKTRVVILQALKKVDAKAQSLYLEIRKMESQLQVLRFESDLDKCISLESSNDIDDRPSSTSPSTLKNPSINSINRQTHDVEKESSDRDVPHLLDPDNNFNLKGNPENPSSTFFTPEILSPKVYSDNVKDEPVEDIFGFEDTSESSPGKPSQLRLQIEAFDDELADTASLGSVTTCLDQWQTFSDDTDSGFALNLYSSEDFRTDPYKEHDEFSMNSLSKWHSATSDN

Target ORF information:

RefSeq Version	NM_001356066.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative guanyl-nucleotide exchange factor Sec73 (sec73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356066.1

ORF Insert Sequence:

ATGACCTTCA GAATTCCTTC CAGCCAGGAG TCTTCAAATA AATCCGAAAA GGATACCACT 
GTAAATTCTC CTAACGCTTC TAGTTTCTCC TCTTTCTTTA AAAAGTCTAG TCAGACATAT 
CTGTCCAAAC GTTCAGAAAA ACTAAATGAG CATAACAGTA AACTAAAGTC AAGCGGATCT 
TTCACAAGTA ATTCACAGAC CGACTTGAGT AATTCGCCCT CTTCTAAGAA AAAAAATATT 
TTTTATCCGA TCGCAAGAGC CAAGCACTCG TTTTACTTCA ACCACAACTG GTCTTCGGAT 
TTGCAGCAAG CAAATTCTCC TGTTGCATCG TATTCAAGTC AAAGCGTTTC AAACGATTCC 
AATTTTCCAA AAGATGTCAC GTCTCCTATT TCGACAGACC TAAGCGGTTC CAATCCTTCT 
TTGAAATCAC CATCTAGTAT CAACTCGGCG AAACTTCGTG CTTCTCGATC ATTTTTTCGA 
TTTCCGAAGT GGTCTAGGAA GTCCTGGAAT CCCGATGTTA ACTCAACTAC TAGTTCTCCT 
TCTGAAGTTG GTTCTCTCGA TAAAGCTGGT GGCCCTCTAA ACCAGCAAAA TGAGAATTTG 
TCAGCGGAAT CTGATAATAC GATTTTACAG AATGGTACTC GGAAAAGTAG TGTCAAAATG 
TCCGACCTGG GGAAACGCAT TGCTTCTTTA CCTGTAGTAA AAAGACCTCC TATCCAGTTT 
TCCATATCTT CTGATTACAT TAAGCCAGTT GATAAAAGCG ATCAGACTTC TAACGTTCCA 
TCACCTTCTT CTTCTGTGGG TTCCCTCCGG TTGTATCGTT CGCCTTCTAT TCCTAATGAT 
GTTGAACAAC CACAGCCACA AAGCTCTCGG TCACGGCCTC GTAGTTCTAC GATTCCTACC 
CAAAGGACTT TAGAAAGATT GAGAGAAAAC CGTTCTAGTA TTTTTAGTTT TCGAACTTTG 
CGCCGGGATG ATGACGAATG CGTAGCTCAG ACTATTGAAA ATTCGGCTTT ATTTTCTGAT 
ATTAAGCCTA ATCCGACCAA GTTTCTTTCT TCTGTTCCGG AGCTCACAGC AGATGATACT 
GCGGAGAGTT ATCTCTCAAA GCTTAGAGAG ACAGTTCCTT ATAGTCGTAT GATCCCTGTT 
CTAGCAGAAA AGGATCATGT AACATTGAAC AAAGCTCTGC ACCTTTGTAT GTCGGGAATG 
GAGTTTGAAA ATCGGCCGTT GGATTTTTGC TTGCGGTTAT TTTTACTTAA ATCTCACCTT 
CCTAAGGAAA CTCAACAGAT TGATCGTGTC ATCAATGCAT TCTCTAAGCG CTATTTTGAA 
TGTAACCCTG GGATGTTCAG TTCCCAAGAT CAGTGTTACA TTCTGGTGTT TTCTTTGATG 
ATGCTCCACA CTGACGTTTT TAATTCAAAT AATAAGCATA AAATGACGAA GCAGGAATTC 
ATAAATCTCT GTGACATAGA TGAATTAGCT CCGGAGATAA TGGAGTATTA TTATGACAAT 
ATTACCTTTA CTCCTTTTGT CAACCTGGAT GATGAGTTGC TTCTCATTGA GAAGGAAAAG 
AACGAAAAGT ACAATTTCAC ATCTCGTAAA AGGTTAGGGC TTTCTCCTTA CACATTTACG 
TTAGAAAATC AATTGAATAT TTCACGGCCT GAAATATCTC TCTCTTATGA TGGAAAATTC 
ATATCTCCAG GTATCAAAGA TATTGCGTCT TTGCTGAACG TTAGAAAGTT GATTTCCAGC 
CCTGCGATAA TTCAGCTTGT TTCTAAAAGA TCTCATCCAA TGGCATTCTC CAATCATTTT 
ATTGGATTAC CAGACAATGC TGACCCGGGA CTAGTTGATT TTAGTATCAG TATGCTGGGC 
ATTTTACCAC GGTGTGAAAA GAAGCGAAGA AGTGTGGGGC ACAATTCATA CAAAGAGCAT 
TTGGTTTTGT TGACCGCTTC TAGAATACTT TTTTTTCGTA ATTTGGTATG GGCTAAAGAT 
CTCATGGCTC AAAAGAAAGC TTATGAAAGG CGTTACAAAA ATTTTACAAC TACCTCGAGC 
AGTTTTGACA CGTTGCAAGG TATAGATACA GAGGCTATGG AATATGAATT TCCAAAAATT 
CCTCCGCTTG TATTTACTCC ACCTCTAGAA GAGTTTTCCC CCGATACCAG TTATTCGCTT 
GTTAATGCGT TTGCCTATTG TGACAGAGAT AAGAAGAATG TACGCAATTG TTCTTTTACC 
GTCATATTTC GGGATGGATC TGAAGAGACT CTTTGTGCTC CAACAGAAGA AAGCATGATA 
GAATGGGTAG CTAAAATTAA CTACATCTCA ACACTTCGTA CAGCTGGTCT GCGAATGCCT 
GTAAGTGATT CTTACCATCC TAAAAGAGAC TTTTCTTGCT ATCCAAAGTA TATGGAGGAA 
GAATTAGCAG CAATTGAAGC TCAAATGAAA CAGGATATGG AAATTGCACG TGCCCGGACA 
ATTAAGACAC GTATTGACCA ACTTGAAACA TCTATTACTG AGTTCAATAA GAAACTCATG 
GTATTACAAA GAAATGCCAA AAATTTATCG ACATGTGCAC CCCTACAGCC GAAAACAAGA 
GTAGTTATTT TACAAGCCCT TAAAAAGGTC GATGCTAAAG CGCAATCATT ATATTTGGAA 
ATCAGAAAAA TGGAGTCACA ATTGCAAGTA TTGCGGTTTG AATCTGATTT AGATAAATGC 
ATTTCTCTTG AAAGTTCTAA TGACATCGAC GACAGACCTT CTTCGACAAG CCCAAGCACC 
CTAAAAAATC CGAGCATCAA CTCAATCAAT CGTCAAACAC ATGATGTTGA AAAAGAATCA 
TCAGATAGAG ATGTACCGCA TCTTTTGGAT CCTGATAATA ATTTCAACTT GAAAGGCAAC 
CCCGAAAACC CCTCATCGAC ATTTTTTACT CCTGAAATAC TTTCACCAAA GGTTTACTCG 
GACAATGTTA AAGATGAGCC TGTCGAAGAT ATTTTTGGTT TTGAAGATAC AAGCGAAAGC 
TCGCCCGGTA AACCCAGCCA ATTGAGGCTT CAAATTGAAG CCTTTGATGA TGAATTAGCT 
GACACAGCTA GCTTAGGTTC CGTGACTACA TGTTTAGATC AATGGCAAAC ATTTTCGGAC 
GATACAGATA GCGGGTTTGC TTTGAACCTG TACTCATCAG AAGATTTTCG CACGGATCCA 
TATAAGGAAC ATGATGAGTT TAGTATGAAT AGCCTATCGA AATGGCATTC TGCTACTTCT 
GATAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec73 ( XM_001713015.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713067.1 Schizosaccharomyces pombe 972h- guanyl-nucleotide exchange factor Sec73 (predicted) (sec73), mRNA.

Price & Availability↑

CloneID	OSj04926
Clone ID Related Accession (Same CDS sequence)	XM_001713015.2
Accession Version	XM_001713015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	guanyl-nucleotide exchange factor Sec73 (predicted)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001713015.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGACCTTCA GAATTCCTTC CAGCCAGGAG TCTTCAAATA AATCCGAAAA GGATACCACT 
GTAAATTCTC CTAACGCTTC TAGTTTCTCC TCTTTCTTTA AAAAGTCTAG TCAGACATAT 
CTGTCCAAAC GTTCAGAAAA ACTAAATGAG CATAACAGTA AACTAAAGTC AAGCGGATCT 
TTCACAAGTA ATTCACAGAC CGACTTGAGT AATTCGCCCT CTTCTAAGAA AAAAAATATT 
TTTTATCCGA TCGCAAGAGC CAAGCACTCG TTTTACTTCA ACCACAACTG GTCTTCGGAT 
TTGCAGCAAG CAAATTCTCC TGTTGCATCG TATTCAAGTC AAAGCGTTTC AAACGATTCC 
AATTTTCCAA AAGATGTCAC GTCTCCTATT TCGACAGACC TAAGCGGTTC CAATCCTTCT 
TTGAAATCAC CATCTAGTAT CAACTCGGCG AAACTTCGTG CTTCTCGATC ATTTTTTCGA 
TTTCCGAAGT GGTCTAGGAA GTCCTGGAAT CCCGATGTTA ACTCAACTAC TAGTTCTCCT 
TCTGAAGTTG GTTCTCTCGA TAAAGCTGGT GGCCCTCTAA ACCAGCAAAA TGAGAATTTG 
TCAGCGGAAT CTGATAATAC GATTTTACAG AATGGTACTC GGAAAAGTAG TGTCAAAATG 
TCCGACCTGG GGAAACGCAT TGCTTCTTTA CCTGTAGTAA AAAGACCTCC TATCCAGTTT 
TCCATATCTT CTGATTACAT TAAGCCAGTT GATAAAAGCG ATCAGACTTC TAACGTTCCA 
TCACCTTCTT CTTCTGTGGG TTCCCTCCGG TTGTATCGTT CGCCTTCTAT TCCTAATGAT 
GTTGAACAAC CACAGCCACA AAGCTCTCGG TCACGGCCTC GTAGTTCTAC GATTCCTACC 
CAAAGGACTT TAGAAAGATT GAGAGAAAAC CGTTCTAGTA TTTTTAGTTT TCGAACTTTG 
CGCCGGGATG ATGACGAATG CGTAGCTCAG ACTATTGAAA ATTCGGCTTT ATTTTCTGAT 
ATTAAGCCTA ATCCGACCAA GTTTCTTTCT TCTGTTCCGG AGCTCACAGC AGATGATACT 
GCGGAGAGTT ATCTCTCAAA GCTTAGAGAG ACAGTTCCTT ATAGTCGTAT GATCCCTGTT 
CTAGCAGAAA AGGATCATGT AACATTGAAC AAAGCTCTGC ACCTTTGTAT GTCGGGAATG 
GAGTTTGAAA ATCGGCCGTT GGATTTTTGC TTGCGGTTAT TTTTACTTAA ATCTCACCTT 
CCTAAGGAAA CTCAACAGAT TGATCGTGTC ATCAATGCAT TCTCTAAGCG CTATTTTGAA 
TGTAACCCTG GGATGTTCAG TTCCCAAGAT CAGTGTTACA TTCTGGTGTT TTCTTTGATG 
ATGCTCCACA CTGACGTTTT TAATTCAAAT AATAAGCATA AAATGACGAA GCAGGAATTC 
ATAAATCTCT GTGACATAGA TGAATTAGCT CCGGAGATAA TGGAGTATTA TTATGACAAT 
ATTACCTTTA CTCCTTTTGT CAACCTGGAT GATGAGTTGC TTCTCATTGA GAAGGAAAAG 
AACGAAAAGT ACAATTTCAC ATCTCGTAAA AGGTTAGGGC TTTCTCCTTA CACATTTACG 
TTAGAAAATC AATTGAATAT TTCACGGCCT GAAATATCTC TCTCTTATGA TGGAAAATTC 
ATATCTCCAG GTATCAAAGA TATTGCGTCT TTGCTGAACG TTAGAAAGTT GATTTCCAGC 
CCTGCGATAA TTCAGCTTGT TTCTAAAAGA TCTCATCCAA TGGCATTCTC CAATCATTTT 
ATTGGATTAC CAGACAATGC TGACCCGGGA CTAGTTGATT TTAGTATCAG TATGCTGGGC 
ATTTTACCAC GGTGTGAAAA GAAGCGAAGA AGTGTGGGGC ACAATTCATA CAAAGAGCAT 
TTGGTTTTGT TGACCGCTTC TAGAATACTT TTTTTTCGTA ATTTGGTATG GGCTAAAGAT 
CTCATGGCTC AAAAGAAAGC TTATGAAAGG CGTTACAAAA ATTTTACAAC TACCTCGAGC 
AGTTTTGACA CGTTGCAAGG TATAGATACA GAGGCTATGG AATATGAATT TCCAAAAATT 
CCTCCGCTTG TATTTACTCC ACCTCTAGAA GAGTTTTCCC CCGATACCAG TTATTCGCTT 
GTTAATGCGT TTGCCTATTG TGACAGAGAT AAGAAGAATG TACGCAATTG TTCTTTTACC 
GTCATATTTC GGGATGGATC TGAAGAGACT CTTTGTGCTC CAACAGAAGA AAGCATGATA 
GAATGGGTAG CTAAAATTAA CTACATCTCA ACACTTCGTA CAGCTGGTCT GCGAATGCCT 
GTAAGTGATT CTTACCATCC TAAAAGAGAC TTTTCTTGCT ATCCAAAGTA TATGGAGGAA 
GAATTAGCAG CAATTGAAGC TCAAATGAAA CAGGATATGG AAATTGCACG TGCCCGGACA 
ATTAAGACAC GTATTGACCA ACTTGAAACA TCTATTACTG AGTTCAATAA GAAACTCATG 
GTATTACAAA GAAATGCCAA AAATTTATCG ACATGTGCAC CCCTACAGCC GAAAACAAGA 
GTAGTTATTT TACAAGCCCT TAAAAAGGTC GATGCTAAAG CGCAATCATT ATATTTGGAA 
ATCAGAAAAA TGGAGTCACA ATTGCAAGTA TTGCGGTTTG AATCTGATTT AGATAAATGC 
ATTTCTCTTG AAAGTTCTAA TGACATCGAC GACAGACCTT CTTCGACAAG CCCAAGCACC 
CTAAAAAATC CGAGCATCAA CTCAATCAAT CGTCAAACAC ATGATGTTGA AAAAGAATCA 
TCAGATAGAG ATGTACCGCA TCTTTTGGAT CCTGATAATA ATTTCAACTT GAAAGGCAAC 
CCCGAAAACC CCTCATCGAC ATTTTTTACT CCTGAAATAC TTTCACCAAA GGTTTACTCG 
GACAATGTTA AAGATGAGCC TGTCGAAGAT ATTTTTGGTT TTGAAGATAC AAGCGAAAGC 
TCGCCCGGTA AACCCAGCCA ATTGAGGCTT CAAATTGAAG CCTTTGATGA TGAATTAGCT 
GACACAGCTA GCTTAGGTTC CGTGACTACA TGTTTAGATC AATGGCAAAC ATTTTCGGAC 
GATACAGATA GCGGGTTTGC TTTGAACCTG TACTCATCAG AAGATTTTCG CACGGATCCA 
TATAAGGAAC ATGATGAGTT TAGTATGAAT AGCCTATCGA AATGGCATTC TGCTACTTCT 
GATAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713067.1
CDS	780..4028
Translation	MTFRIPSSQESSNKSEKDTTVNSPNASSFSSFFKKSSQTYLSKRSEKLNEHNSKLKSSGSFTSNSQTDLSNSPSSKKKNIFYPIARAKHSFYFNHNWSSDLQQANSPVASYSSQSVSNDSNFPKDVTSPISTDLSGSNPSLKSPSSINSAKLRASRSFFRFPKWSRKSWNPDVNSTTSSPSEVGSLDKAGGPLNQQNENLSAESDNTILQNGTRKSSVKMSDLGKRIASLPVVKRPPIQFSISSDYIKPVDKSDQTSNVPSPSSSVGSLRLYRSPSIPNDVEQPQPQSSRSRPRSSTIPTQRTLERLRENRSSIFSFRTLRRDDDECVAQTIENSALFSDIKPNPTKFLSSVPELTADDTAESYLSKLRETVPYSRMIPVLAEKDHVTLNKALHLCMSGMEFENRPLDFCLRLFLLKSHLPKETQQIDRVINAFSKRYFECNPGMFSSQDQCYILVFSLMMLHTDVFNSNNKHKMTKQEFINLCDIDELAPEIMEYYYDNITFTPFVNLDDELLLIEKEKNEKYNFTSRKRLGLSPYTFTLENQLNISRPEISLSYDGKFISPGIKDIASLLNVRKLISSPAIIQLVSKRSHPMAFSNHFIGLPDNADPGLVDFSISMLGILPRCEKKRRSVGHNSYKEHLVLLTASRILFFRNLVWAKDLMAQKKAYERRYKNFTTTSSSFDTLQGIDTEAMEYEFPKIPPLVFTPPLEEFSPDTSYSLVNAFAYCDRDKKNVRNCSFTVIFRDGSEETLCAPTEESMIEWVAKINYISTLRTAGLRMPVSDSYHPKRDFSCYPKYMEEELAAIEAQMKQDMEIARARTIKTRIDQLETSITEFNKKLMVLQRNAKNLSTCAPLQPKTRVVILQALKKVDAKAQSLYLEIRKMESQLQVLRFESDLDKCISLESSNDIDDRPSSTSPSTLKNPSINSINRQTHDVEKESSDRDVPHLLDPDNNFNLKGNPENPSSTFFTPEILSPKVYSDNVKDEPVEDIFGFEDTSESSPGKPSQLRLQIEAFDDELADTASLGSVTTCLDQWQTFSDDTDSGFALNLYSSEDFRTDPYKEHDEFSMNSLSKWHSATSDN

Target ORF information:

RefSeq Version	XM_001713015.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- guanyl-nucleotide exchange factor Sec73 (predicted) (sec73), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713015.2

ORF Insert Sequence:

ATGACCTTCA GAATTCCTTC CAGCCAGGAG TCTTCAAATA AATCCGAAAA GGATACCACT 
GTAAATTCTC CTAACGCTTC TAGTTTCTCC TCTTTCTTTA AAAAGTCTAG TCAGACATAT 
CTGTCCAAAC GTTCAGAAAA ACTAAATGAG CATAACAGTA AACTAAAGTC AAGCGGATCT 
TTCACAAGTA ATTCACAGAC CGACTTGAGT AATTCGCCCT CTTCTAAGAA AAAAAATATT 
TTTTATCCGA TCGCAAGAGC CAAGCACTCG TTTTACTTCA ACCACAACTG GTCTTCGGAT 
TTGCAGCAAG CAAATTCTCC TGTTGCATCG TATTCAAGTC AAAGCGTTTC AAACGATTCC 
AATTTTCCAA AAGATGTCAC GTCTCCTATT TCGACAGACC TAAGCGGTTC CAATCCTTCT 
TTGAAATCAC CATCTAGTAT CAACTCGGCG AAACTTCGTG CTTCTCGATC ATTTTTTCGA 
TTTCCGAAGT GGTCTAGGAA GTCCTGGAAT CCCGATGTTA ACTCAACTAC TAGTTCTCCT 
TCTGAAGTTG GTTCTCTCGA TAAAGCTGGT GGCCCTCTAA ACCAGCAAAA TGAGAATTTG 
TCAGCGGAAT CTGATAATAC GATTTTACAG AATGGTACTC GGAAAAGTAG TGTCAAAATG 
TCCGACCTGG GGAAACGCAT TGCTTCTTTA CCTGTAGTAA AAAGACCTCC TATCCAGTTT 
TCCATATCTT CTGATTACAT TAAGCCAGTT GATAAAAGCG ATCAGACTTC TAACGTTCCA 
TCACCTTCTT CTTCTGTGGG TTCCCTCCGG TTGTATCGTT CGCCTTCTAT TCCTAATGAT 
GTTGAACAAC CACAGCCACA AAGCTCTCGG TCACGGCCTC GTAGTTCTAC GATTCCTACC 
CAAAGGACTT TAGAAAGATT GAGAGAAAAC CGTTCTAGTA TTTTTAGTTT TCGAACTTTG 
CGCCGGGATG ATGACGAATG CGTAGCTCAG ACTATTGAAA ATTCGGCTTT ATTTTCTGAT 
ATTAAGCCTA ATCCGACCAA GTTTCTTTCT TCTGTTCCGG AGCTCACAGC AGATGATACT 
GCGGAGAGTT ATCTCTCAAA GCTTAGAGAG ACAGTTCCTT ATAGTCGTAT GATCCCTGTT 
CTAGCAGAAA AGGATCATGT AACATTGAAC AAAGCTCTGC ACCTTTGTAT GTCGGGAATG 
GAGTTTGAAA ATCGGCCGTT GGATTTTTGC TTGCGGTTAT TTTTACTTAA ATCTCACCTT 
CCTAAGGAAA CTCAACAGAT TGATCGTGTC ATCAATGCAT TCTCTAAGCG CTATTTTGAA 
TGTAACCCTG GGATGTTCAG TTCCCAAGAT CAGTGTTACA TTCTGGTGTT TTCTTTGATG 
ATGCTCCACA CTGACGTTTT TAATTCAAAT AATAAGCATA AAATGACGAA GCAGGAATTC 
ATAAATCTCT GTGACATAGA TGAATTAGCT CCGGAGATAA TGGAGTATTA TTATGACAAT 
ATTACCTTTA CTCCTTTTGT CAACCTGGAT GATGAGTTGC TTCTCATTGA GAAGGAAAAG 
AACGAAAAGT ACAATTTCAC ATCTCGTAAA AGGTTAGGGC TTTCTCCTTA CACATTTACG 
TTAGAAAATC AATTGAATAT TTCACGGCCT GAAATATCTC TCTCTTATGA TGGAAAATTC 
ATATCTCCAG GTATCAAAGA TATTGCGTCT TTGCTGAACG TTAGAAAGTT GATTTCCAGC 
CCTGCGATAA TTCAGCTTGT TTCTAAAAGA TCTCATCCAA TGGCATTCTC CAATCATTTT 
ATTGGATTAC CAGACAATGC TGACCCGGGA CTAGTTGATT TTAGTATCAG TATGCTGGGC 
ATTTTACCAC GGTGTGAAAA GAAGCGAAGA AGTGTGGGGC ACAATTCATA CAAAGAGCAT 
TTGGTTTTGT TGACCGCTTC TAGAATACTT TTTTTTCGTA ATTTGGTATG GGCTAAAGAT 
CTCATGGCTC AAAAGAAAGC TTATGAAAGG CGTTACAAAA ATTTTACAAC TACCTCGAGC 
AGTTTTGACA CGTTGCAAGG TATAGATACA GAGGCTATGG AATATGAATT TCCAAAAATT 
CCTCCGCTTG TATTTACTCC ACCTCTAGAA GAGTTTTCCC CCGATACCAG TTATTCGCTT 
GTTAATGCGT TTGCCTATTG TGACAGAGAT AAGAAGAATG TACGCAATTG TTCTTTTACC 
GTCATATTTC GGGATGGATC TGAAGAGACT CTTTGTGCTC CAACAGAAGA AAGCATGATA 
GAATGGGTAG CTAAAATTAA CTACATCTCA ACACTTCGTA CAGCTGGTCT GCGAATGCCT 
GTAAGTGATT CTTACCATCC TAAAAGAGAC TTTTCTTGCT ATCCAAAGTA TATGGAGGAA 
GAATTAGCAG CAATTGAAGC TCAAATGAAA CAGGATATGG AAATTGCACG TGCCCGGACA 
ATTAAGACAC GTATTGACCA ACTTGAAACA TCTATTACTG AGTTCAATAA GAAACTCATG 
GTATTACAAA GAAATGCCAA AAATTTATCG ACATGTGCAC CCCTACAGCC GAAAACAAGA 
GTAGTTATTT TACAAGCCCT TAAAAAGGTC GATGCTAAAG CGCAATCATT ATATTTGGAA 
ATCAGAAAAA TGGAGTCACA ATTGCAAGTA TTGCGGTTTG AATCTGATTT AGATAAATGC 
ATTTCTCTTG AAAGTTCTAA TGACATCGAC GACAGACCTT CTTCGACAAG CCCAAGCACC 
CTAAAAAATC CGAGCATCAA CTCAATCAAT CGTCAAACAC ATGATGTTGA AAAAGAATCA 
TCAGATAGAG ATGTACCGCA TCTTTTGGAT CCTGATAATA ATTTCAACTT GAAAGGCAAC 
CCCGAAAACC CCTCATCGAC ATTTTTTACT CCTGAAATAC TTTCACCAAA GGTTTACTCG 
GACAATGTTA AAGATGAGCC TGTCGAAGAT ATTTTTGGTT TTGAAGATAC AAGCGAAAGC 
TCGCCCGGTA AACCCAGCCA ATTGAGGCTT CAAATTGAAG CCTTTGATGA TGAATTAGCT 
GACACAGCTA GCTTAGGTTC CGTGACTACA TGTTTAGATC AATGGCAAAC ATTTTCGGAC 
GATACAGATA GCGGGTTTGC TTTGAACCTG TACTCATCAG AAGATTTTCG CACGGATCCA 
TATAAGGAAC ATGATGAGTT TAGTATGAAT AGCCTATCGA AATGGCATTC TGCTACTTCT 
GATAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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sec73 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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