SPAC12G12.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC12G12.01c
Entrez Gene ID	3361381
Full Name	ubiquitin-protein ligase E3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001712987.2	XP_001713039.1	ubiquitin-protein ligase E3
NM_001355976.1	NP_001342800.1	ubiquitin-protein ligase E3

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC12G12.01c	XP_001713039.1
Eremothecium gossypii	AGOS_ACR192C	NP_983594.1
Saccharomyces cerevisiae (baker's yeast)	SEA4	NP_009446.2
Kluyveromyces lactis	KLLA0E06469g	XP_454239.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC12G12.01c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC12G12.01c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj04875	XM_001712987.2 Latest version!	Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OSc101738	NM_001355976.1 Latest version!	Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC12G12.01c ( XM_001712987.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713039.1 Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.

Price & Availability↑

CloneID	OSj04875
Clone ID Related Accession (Same CDS sequence)	XM_001712987.2
Accession Version	XM_001712987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	ubiquitin-protein ligase E3
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001712987.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
GAATGCAAAC TGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713039.1
CDS	401..3118
Translation	MLNRSKKRNGCYFWVNGSSDEIRYVAVKASPKVNWKTHIIWRSLKNVKCIDSFHGNNEILGAGSSTGNISLLSVKHPEFQAVVTPGYARPCNSLAFSETEHLVAGFAKSRNESSLKLWDLNSLLSDPKSSPLMQSSTLDGVSSVCYKKDTPLLLTGSTSRSVHIIDTRQQLDSVSSVNTQYYSNIVVDPFSPNYFAANSYDGDIAIFDTRYFKSDNYLQIILRNENKKPKNPQLFALKYSEWKPGQLAVLSNNSITLRQLLPCVNGNEGSANNSVFVNYEKKYPVKPNSQCSGIDFFTPSTAFPTHVQILGVINEQPKLFSVHDEVIPFSFNPYNDLIFSFKEKLYPLNSSPFNTLSDVPQFDVSEFVDENSFDSSSSCSSKVFLTTRNNSINSEDSAHEVLLSYNRVLGSDIQGTILDRVKKGYQFDSQKNSELVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQGALGIWFMDTELTSMSDVFEAKESKFLEKKILRLARDVIERLDLDIFTSIQTKRPLRQLALLACGLGMSNDDLLLEIRRLIRKNEHVKAAGLALFHGKIENVVRILSSGNELEKTISTAVAGYITSQGLSNFGSDSLWKEMSRNLSTELEDPYLRAIFAYVSNSDWRDVLDEVSLSLKDRLGIALRFLPDDDLSNYLCDLCHTTVQSGDPEGLLLTGLTPLGMELLQNYIDHTSDVQTAALIAAFVVPKKFLDKRAEDWTESYRELLNRWKLYRERAKFDIFRTELSKNHTGEITRKATEPSIRIICNFCRKPIFPFSNRNECNNLPTPIQRGVSKAGPAKHLGKSCPHCGQPLPKCSVCGFSLGDEDVPQKDDFSQKPQNYVKEVNLQKSRFGLWFSFCLNCGHGAHASHASEWFSTHTICPVPNCDCECKLK

Target ORF information:

RefSeq Version	XM_001712987.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001712987.2

ORF Insert Sequence:

ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
GAATGCAAAC TGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAC12G12.01c ( NM_001355976.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342800.1 Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.

Price & Availability↑

CloneID	OSc101738
Clone ID Related Accession (Same CDS sequence)	NM_001355976.1
Accession Version	NM_001355976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	ubiquitin-protein ligase E3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
GAATGCAAAC TGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342800.1
CDS	401..3118
Translation	MLNRSKKRNGCYFWVNGSSDEIRYVAVKASPKVNWKTHIIWRSLKNVKCIDSFHGNNEILGAGSSTGNISLLSVKHPEFQAVVTPGYARPCNSLAFSETEHLVAGFAKSRNESSLKLWDLNSLLSDPKSSPLMQSSTLDGVSSVCYKKDTPLLLTGSTSRSVHIIDTRQQLDSVSSVNTQYYSNIVVDPFSPNYFAANSYDGDIAIFDTRYFKSDNYLQIILRNENKKPKNPQLFALKYSEWKPGQLAVLSNNSITLRQLLPCVNGNEGSANNSVFVNYEKKYPVKPNSQCSGIDFFTPSTAFPTHVQILGVINEQPKLFSVHDEVIPFSFNPYNDLIFSFKEKLYPLNSSPFNTLSDVPQFDVSEFVDENSFDSSSSCSSKVFLTTRNNSINSEDSAHEVLLSYNRVLGSDIQGTILDRVKKGYQFDSQKNSELVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQGALGIWFMDTELTSMSDVFEAKESKFLEKKILRLARDVIERLDLDIFTSIQTKRPLRQLALLACGLGMSNDDLLLEIRRLIRKNEHVKAAGLALFHGKIENVVRILSSGNELEKTISTAVAGYITSQGLSNFGSDSLWKEMSRNLSTELEDPYLRAIFAYVSNSDWRDVLDEVSLSLKDRLGIALRFLPDDDLSNYLCDLCHTTVQSGDPEGLLLTGLTPLGMELLQNYIDHTSDVQTAALIAAFVVPKKFLDKRAEDWTESYRELLNRWKLYRERAKFDIFRTELSKNHTGEITRKATEPSIRIICNFCRKPIFPFSNRNECNNLPTPIQRGVSKAGPAKHLGKSCPHCGQPLPKCSVCGFSLGDEDVPQKDDFSQKPQNYVKEVNLQKSRFGLWFSFCLNCGHGAHASHASEWFSTHTICPVPNCDCECKLK

Target ORF information:

RefSeq Version	NM_001355976.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355976.1

ORF Insert Sequence:

ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
GAATGCAAAC TGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC12G12.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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