mis4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mis4
Entrez Gene ID	2542657
Full Name	adherin, cohesin loading factor Mis4
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018318.2	NP_592917.2	adherin Mis4

Pathways from BioSystems

Homologies

Neurospora crassa	NCU05250	XP_961787.1
Magnaporthe oryzae (rice blast fungus)	MGG_01726	XP_003714723.1
Schizosaccharomyces pombe (fission yeast)	mis4	NP_592917.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mis4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mis4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101744	NM_001018318.2 Latest version!	Schizosaccharomyces pombe adherin Mis4 (mis4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mis4 ( NM_001018318.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_592917.2 Schizosaccharomyces pombe adherin Mis4 (mis4), mRNA.

Price & Availability↑

CloneID	OSc101744
Clone ID Related Accession (Same CDS sequence)	NM_001018318.2
Accession Version	NM_001018318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	adherin Mis4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018318.1.

ATGCTTTTCG AGATGACACC TGAAACCTTT AAAAAAGTTA ACTCCTCTAA TCGAATTATT 
AAAGGGCTTC AATACACACC ATTAGCATCA TCAATCCCAT TGGAAAATGG TTTACAAAAC 
GTATTATATC CATCTTCTAA ATTTCAAAAT GAGCCATTGC AACTTAACTC CGAAGAATCA 
AGTATTATGC AAAGATATGT GGATATGCTA AATCCTGGCG CAACATTCGT TAACGATTCT 
GAAACCTTTA ATTTTTATAA AAATGCTCTT AGCGCTATGA TAACGGAACC TGCAATGCCC 
GCAATGAGGG TGAATGCCAG TCCGGTCCTA GACCAAAAGG TGTGTAACAG TGACTCGTTA 
AGTGAATTAA ATGATTTTAC AAAATCTCTG ATTCAGCCAA GTATGTTGAT GTGTGAACCC 
AAGGAGAAAC CTGATGCTTC TTCTATAAAT ACAAACCGTT CATCTTCCGA CAATGGCTTC 
TTAACTCCGT CTTCAAGTCC TCGAAGTCCA TCATGTTCTC GCGTATTTAA CGCTGTTCAA 
TTATGTAGCC CTAAAAAATC TAAAGATGAT ATTACTACCC CAAAAAAGCG ACTTATGGAA 
GACACTTATT CGCCTCGTGA ATCTCCCTCA AAGATCCAGC GGTTGCAGGA CGTTTTGTTA 
AAGCAGTTGC AAGATACCAG ATTACTAATT TCTCAAGTTA TAGAAGCTGA GAATTCGGAA 
GATTTTTCAT CGAATTCTCT CTTTATTAAA CGAGAAGACG ATGATGGAAA ACACATTTCA 
TCGCACGCAA TTGAAAAGCT ATATATGGCA TTAACCAAGC TTAGTCGTTT GGGAGCTTGT 
GACAAATTGC TGGAAGAAGG GAGTATAATA CTTGTAAAAC AAATCCTTGA GAAAGAGTTA 
AAAGAGCTTC CTGTCGCTTG TTACAGTATA ATTAATCTTC ATGACAGCCT CACACAATTT 
CCGAATTTAG ACTTTATTTT AAAGACCACT GCTCTTGTTC TTTTTATAAT ATTTCTGGTC 
CCCTCCTTTA AAAAGCTTCA AAATGAAGAA TCAATTCTTC ATTTACTTAA TATCTTACAT 
TCCATATTTG AGTACACAGT TCCTGAAGCG ATTGATAACA TCGTTCAATC AAAAACTTCC 
GATGCTAGGA CTTCTGAAAT TCAACATCTA TCAGTTTTGT TACAAAAAGT CGCAAATGTA 
TTAAACATCT TATCTAAAGT TGCCCATGAA ATTCCCTTGT CGGAAGCTGT GGTCATACGC 
ATTGTATATT TATTTCCCAA AGTCTCTACA CTCGACAATT CCTTCAAAAC TAAACTTCCT 
AACTGTAACA GCTCATCCTT CGATTTTCTT AAAGCTCCTT TATTTCAAAC GTTACAATAT 
TTATTTCGTC TTTATCCTTA TCAGCGAGAT TTTATCATTG AAGAATCACT TACAAATTTT 
TCTCACTTGC CCACTGCTCG TTCGGTCTCA AGGACCTATA GACTCTCTAA CGGTAAATCC 
ATACAATACT ATTCAACCCT ATTTGTACGT TTAATTCAGT CATGCTCCAT TCAAAACTTA 
TTCGACTCAG AAATTGTACA ATCGGAGAGC AAATCCACTG AAGCTTTACA CTCAGGTAAC 
TTAACAGAAC ATCTGAAAAC AGTCGAATCA ATACTATCCA AGTCTCGACA TGAAGAATAC 
CGGATAGCTA ATCATATTGT TGCTTATTTG CTTAGTCGTT CTTTAAAACA GAATAAAACG 
GAGAGTGACA ACTCATTTGC AATTTTAACC AAAATCCTTT TAGAAGATTT ATTAAACATG 
CTTTCTTTGC CTGAATGGTG CGGAACCGAA ACAATTATTC GGCAATTTGC AATGAATTTA 
GTTATGACTG TGACTAACGA CAAACAAGCA GTTTCTTCAA AGAATGCTGC GCTGGATCTT 
ATAAGTCTCA TCGTTAACAA GGTTTTGGCA TTGTTTGATC TTTCCCTTTT CGAGAAGCAC 
AATATCCCAG CTCCTACGAA TTTCAATGAT ATAATATCGT TCATTCCCTC AATTACTAGA 
TTAAATGAAC TTTCTCAAGT TAGCTTTAAC CATTTTTACT TTTTATGCAA AGGTGACATA 
TCTCTGGAAA ACATACTCCC TTATAACTAC AATAAATGGT TTTCATTTCT CTTACAGCTT 
AGAAAAGTAT GCAATGACTC CGAAGCTTTA AAAATTATTG ATAATTGTAT CGATAAGAAT 
ATGCAAAAGT CTCAAGAGGG GTTTCAAGGT CCTTCACCAT TTAAAGCAGA TGAAAATGAC 
GAAGATATTT TCATTATATC CCTTTATCAT TCTTCTTTGT TTTTAAATTT AAAGTTTTTT 
GTCTCGCTCA TTATAGGCTT TCTCGATAGT CCTCAAGCTA GTCTTCGAAC TAAATGCCTT 
CGAATCATCA ATCAAATGAA AACTATACCT TCTATATTAA GAACACATCC AGAAGTTTTG 
GCTCAGATAA TATCAAAATC GAATGATCAG TCTGCGATAG TTCGAGATAC TGTGTTAGAC 
TTACTTGGCA CATATATCAT GGCATATCGG GAGACAATTC CTCAAATATA TGGTTGTATA 
ATTTCTGGAA TTTCGGATCC TTCAACAATC GTTCGTAAAC GCGCTATAAA GCAACTATGT 
GAGGTTTATG AAGCTACCGA AGATTTAAAT ATCCGTGTTG ACATAGCTTC AAAACTTTTA 
ACCCGGAGTA ATGATGAAGA GGAAACAATC AGTGAATTAA GCCTTGAGGT TTTAGAAAAA 
CTTTGGTTTT CACCCGCAAG CAATGAATTA GATTGTCAAA AAGGCTATGA GCAATTAACT 
TTCTTAGAAA AGCAGAAGTT AAGAGTTCAG TATTTTCCTA TTTTAAAACT ATGTGCTGAA 
CCATCAACCG AAAGACACGT CCTGTTGGTA ACGTCTCTGA AAACCATGTT GACTTCTAAG 
GAAGAGATCA ATTTGAGTAC ACTACACACT CAAATTCGAC TTCTTCTCTC TTGTCTTTTC 
AATCAGCTAA TAGAAGTTGT AACCGAAGAT CAAGTTGATG AATCAACAAA GGGAATTTTA 
TACGAAATAA TGAGTACATT GTTTGTTTTT TCACGCGCTT TTCCCTTCCT CTTTGACTTG 
TCTTATCTAC ACCTCTTAAA ACCCTATTTA CGATCTGCTT CAACTATTGA AGAGCAACGT 
TTTCTTTACT ATGTAGTAGC TATTTTTAGA CAGGTTTTAC CATTTCAAAA AGAAATTTCT 
GAAAGCTTCC TACGGAGCCT TGAATCTGTG TTACTGCAAA GGCTTACTAA AGCCGGTACT 
GCAACGTTAA TGGAAATAGT TCCTTGTCTT TGCAGCCTAT TTACAAGACT TAATGATTAT 
GAAAGGTTAA AGAAAATTGT GGTTTCGTGT CTTAAATCTT TAGAGGAGGC ACGACACTCA 
GAAAACAATT TTCAAAAAAT GGTTCGCTTA ATAGACCTCA TTGGGCTTTT TTCTAGATAT 
GGGGATCTCA ATAGAATAAA TGATGATTGG AAACATTCAC TTGATTTTAT TAGTCCTGAG 
TGTGATGATG CTTACGTTAT ACTATTAGGT TATTTTCAGA AACTTCTAAA AGATGCGAAA 
GGTCAGCTGA GAATTCACAT AATTGATAAC ATGAGCAGAA TATGTTTAAG AGAAACATCT 
TTGTTTATAT CTCCTCTTAT GTTATCAACT TTGGATATGA TAATTGCGGA AAATAATGTT 
AACGAAGTAT CTGTTTTGTT TAAATCTTTT CTTGAGCTTT TGGCCGCGGA TGAGGATCTT 
ATCTTTGAAG CAGATCAAAA GCTAAGTTTA AAGGGTAAGC AAAACGTTCA GTCCAACAAA 
TCAGTTGATC GAGATATGCT TAAAGGAACT AAGGATAAAC AATGGATTGA AGGAGTCAGT 
GCATCTTTAA TGCAGCATTT CCTTCCCTGT ATTCTTGACT CTTGCTTTTC AAAAAATTTA 
AGGTATTCAA TGCTCGGAAT TGAAATTCTC AAATGTATTA TTCATCAAGG ACTAGTAAAC 
CCACGCATGT GTTTCTCAAC CATAATAGCA CTCGAATCCA ATGCAATTAA AGAAACCAGA 
GAAGTAGCAA TTTTGCTTCA CACTGAATTG CACCGAAGAC ATGAATCTTT GATAGATGGC 
TTATATGCTC AATCTGCAGA TTTAATATTT TCCCTTCAAA AAACGGAGGA ATATCAAACA 
TTTAAGTTAG GCGAATTTTC GCCGTTCCAA AGTGCCTATA CCATTGTCTC CGCTGATAAA 
AGTTCAAAAT CTAGGAAAAA ATTAATAATG CAAATATTAA AACCTCTTAA ACTCGACGGG 
ATTGATTTAC CCAGCTTTAC AGAAGAAAAA GTTTCATTTG TAAGCTTTTG CTGTGTATGT 
CTGGCAGGAA TTCCATACGT GTCAATAGAG GAACCGTTAA TGATCATTTC AACAGTTGAT 
TCGGTGCTCG CGACGATAGG CCCTACTATT ACTGGGTGGA TGAAAAAACT AGATCATGAA 
CGTTTTAAAA TCCTTGCAGG TATCAACTTG TGCAATTTGA TATATCTTAA GAGATATATA 
AAATACGCAT TTTCAATTTC AGACTCATCT CGACCTATAC GTGAGAAAAA GCCTCTAACT 
CTTTTGAATA GAGGTTATGT GGATTTAATT ACTTCTGATG CTAAGCCAGA TATTGTTTCT 
AAGTTGGTGA TAAAATTGTT TGAAGAAGAA AATATCCTTT CTGGCGAGGA TCAAGTTGAA 
GGGGAACAGC TTACAGTTGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_592917.2
CDS	111..4874
Translation	MLFEMTPETFKKVNSSNRIIKGLQYTPLASSIPLENGLQNVLYPSSKFQNEPLQLNSEESSIMQRYVDMLNPGATFVNDSETFNFYKNALSAMITEPAMPAMRVNASPVLDQKVCNSDSLSELNDFTKSLIQPSMLMCEPKEKPDASSINTNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDITTPKKRLMEDTYSPRESPSKIQRLQDVLLKQLQDTRLLISQVIEAENSEDFSSNSLFIKREDDDGKHISSHAIEKLYMALTKLSRLGACDKLLEEGSIILVKQILEKELKELPVACYSIINLHDSLTQFPNLDFILKTTALVLFIIFLVPSFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQKVANVLNILSKVAHEIPLSEAVVIRIVYLFPKVSTLDNSFKTKLPNCNSSSFDFLKAPLFQTLQYLFRLYPYQRDFIIEESLTNFSHLPTARSVSRTYRLSNGKSIQYYSTLFVRLIQSCSIQNLFDSEIVQSESKSTEALHSGNLTEHLKTVESILSKSRHEEYRIANHIVAYLLSRSLKQNKTESDNSFAILTKILLEDLLNMLSLPEWCGTETIIRQFAMNLVMTVTNDKQAVSSKNAALDLISLIVNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYFLCKGDISLENILPYNYNKWFSFLLQLRKVCNDSEALKIIDNCIDKNMQKSQEGFQGPSPFKADENDEDIFIISLYHSSLFLNLKFFVSLIIGFLDSPQASLRTKCLRIINQMKTIPSILRTHPEVLAQIISKSNDQSAIVRDTVLDLLGTYIMAYRETIPQIYGCIISGISDPSTIVRKRAIKQLCEVYEATEDLNIRVDIASKLLTRSNDEEETISELSLEVLEKLWFSPASNELDCQKGYEQLTFLEKQKLRVQYFPILKLCAEPSTERHVLLVTSLKTMLTSKEEINLSTLHTQIRLLLSCLFNQLIEVVTEDQVDESTKGILYEIMSTLFVFSRAFPFLFDLSYLHLLKPYLRSASTIEEQRFLYYVVAIFRQVLPFQKEISESFLRSLESVLLQRLTKAGTATLMEIVPCLCSLFTRLNDYERLKKIVVSCLKSLEEARHSENNFQKMVRLIDLIGLFSRYGDLNRINDDWKHSLDFISPECDDAYVILLGYFQKLLKDAKGQLRIHIIDNMSRICLRETSLFISPLMLSTLDMIIAENNVNEVSVLFKSFLELLAADEDLIFEADQKLSLKGKQNVQSNKSVDRDMLKGTKDKQWIEGVSASLMQHFLPCILDSCFSKNLRYSMLGIEILKCIIHQGLVNPRMCFSTIIALESNAIKETREVAILLHTELHRRHESLIDGLYAQSADLIFSLQKTEEYQTFKLGEFSPFQSAYTIVSADKSSKSRKKLIMQILKPLKLDGIDLPSFTEEKVSFVSFCCVCLAGIPYVSIEEPLMIISTVDSVLATIGPTITGWMKKLDHERFKILAGINLCNLIYLKRYIKYAFSISDSSRPIREKKPLTLLNRGYVDLITSDAKPDIVSKLVIKLFEEENILSGEDQVEGEQLTVV

Target ORF information:

RefSeq Version	NM_001018318.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe adherin Mis4 (mis4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018318.2

ORF Insert Sequence:

ATGCTTTTCG AGATGACACC TGAAACCTTT AAAAAAGTTA ACTCCTCTAA TCGAATTATT 
AAAGGGCTTC AATACACACC ATTAGCATCA TCAATCCCAT TGGAAAATGG TTTACAAAAC 
GTATTATATC CATCTTCTAA ATTTCAAAAT GAGCCATTGC AACTTAACTC CGAAGAATCA 
AGTATTATGC AAAGATATGT GGATATGCTA AATCCTGGCG CAACATTCGT TAACGATTCT 
GAAACCTTTA ATTTTTATAA AAATGCTCTT AGCGCTATGA TAACGGAACC TGCAATGCCC 
GCAATGAGGG TGAATGCCAG TCCGGTCCTA GACCAAAAGG TGTGTAACAG TGACTCGTTA 
AGTGAATTAA ATGATTTTAC AAAATCTCTG ATTCAGCCAA GTATGTTGAT GTGTGAACCC 
AAGGAGAAAC CTGATGCTTC TTCTATAAAT ACAAACCGTT CATCTTCCGA CAATGGCTTC 
TTAACTCCGT CTTCAAGTCC TCGAAGTCCA TCATGTTCTC GCGTATTTAA CGCTGTTCAA 
TTATGTAGCC CTAAAAAATC TAAAGATGAT ATTACTACCC CAAAAAAGCG ACTTATGGAA 
GACACTTATT CGCCTCGTGA ATCTCCCTCA AAGATCCAGC GGTTGCAGGA CGTTTTGTTA 
AAGCAGTTGC AAGATACCAG ATTACTAATT TCTCAAGTTA TAGAAGCTGA GAATTCGGAA 
GATTTTTCAT CGAATTCTCT CTTTATTAAA CGAGAAGACG ATGATGGAAA ACACATTTCA 
TCGCACGCAA TTGAAAAGCT ATATATGGCA TTAACCAAGC TTAGTCGTTT GGGAGCTTGT 
GACAAATTGC TGGAAGAAGG GAGTATAATA CTTGTAAAAC AAATCCTTGA GAAAGAGTTA 
AAAGAGCTTC CTGTCGCTTG TTACAGTATA ATTAATCTTC ATGACAGCCT CACACAATTT 
CCGAATTTAG ACTTTATTTT AAAGACCACT GCTCTTGTTC TTTTTATAAT ATTTCTGGTC 
CCCTCCTTTA AAAAGCTTCA AAATGAAGAA TCAATTCTTC ATTTACTTAA TATCTTACAT 
TCCATATTTG AGTACACAGT TCCTGAAGCG ATTGATAACA TCGTTCAATC AAAAACTTCC 
GATGCTAGGA CTTCTGAAAT TCAACATCTA TCAGTTTTGT TACAAAAAGT CGCAAATGTA 
TTAAACATCT TATCTAAAGT TGCCCATGAA ATTCCCTTGT CGGAAGCTGT GGTCATACGC 
ATTGTATATT TATTTCCCAA AGTCTCTACA CTCGACAATT CCTTCAAAAC TAAACTTCCT 
AACTGTAACA GCTCATCCTT CGATTTTCTT AAAGCTCCTT TATTTCAAAC GTTACAATAT 
TTATTTCGTC TTTATCCTTA TCAGCGAGAT TTTATCATTG AAGAATCACT TACAAATTTT 
TCTCACTTGC CCACTGCTCG TTCGGTCTCA AGGACCTATA GACTCTCTAA CGGTAAATCC 
ATACAATACT ATTCAACCCT ATTTGTACGT TTAATTCAGT CATGCTCCAT TCAAAACTTA 
TTCGACTCAG AAATTGTACA ATCGGAGAGC AAATCCACTG AAGCTTTACA CTCAGGTAAC 
TTAACAGAAC ATCTGAAAAC AGTCGAATCA ATACTATCCA AGTCTCGACA TGAAGAATAC 
CGGATAGCTA ATCATATTGT TGCTTATTTG CTTAGTCGTT CTTTAAAACA GAATAAAACG 
GAGAGTGACA ACTCATTTGC AATTTTAACC AAAATCCTTT TAGAAGATTT ATTAAACATG 
CTTTCTTTGC CTGAATGGTG CGGAACCGAA ACAATTATTC GGCAATTTGC AATGAATTTA 
GTTATGACTG TGACTAACGA CAAACAAGCA GTTTCTTCAA AGAATGCTGC GCTGGATCTT 
ATAAGTCTCA TCGTTAACAA GGTTTTGGCA TTGTTTGATC TTTCCCTTTT CGAGAAGCAC 
AATATCCCAG CTCCTACGAA TTTCAATGAT ATAATATCGT TCATTCCCTC AATTACTAGA 
TTAAATGAAC TTTCTCAAGT TAGCTTTAAC CATTTTTACT TTTTATGCAA AGGTGACATA 
TCTCTGGAAA ACATACTCCC TTATAACTAC AATAAATGGT TTTCATTTCT CTTACAGCTT 
AGAAAAGTAT GCAATGACTC CGAAGCTTTA AAAATTATTG ATAATTGTAT CGATAAGAAT 
ATGCAAAAGT CTCAAGAGGG GTTTCAAGGT CCTTCACCAT TTAAAGCAGA TGAAAATGAC 
GAAGATATTT TCATTATATC CCTTTATCAT TCTTCTTTGT TTTTAAATTT AAAGTTTTTT 
GTCTCGCTCA TTATAGGCTT TCTCGATAGT CCTCAAGCTA GTCTTCGAAC TAAATGCCTT 
CGAATCATCA ATCAAATGAA AACTATACCT TCTATATTAA GAACACATCC AGAAGTTTTG 
GCTCAGATAA TATCAAAATC GAATGATCAG TCTGCGATAG TTCGAGATAC TGTGTTAGAC 
TTACTTGGCA CATATATCAT GGCATATCGG GAGACAATTC CTCAAATATA TGGTTGTATA 
ATTTCTGGAA TTTCGGATCC TTCAACAATC GTTCGTAAAC GCGCTATAAA GCAACTATGT 
GAGGTTTATG AAGCTACCGA AGATTTAAAT ATCCGTGTTG ACATAGCTTC AAAACTTTTA 
ACCCGGAGTA ATGATGAAGA GGAAACAATC AGTGAATTAA GCCTTGAGGT TTTAGAAAAA 
CTTTGGTTTT CACCCGCAAG CAATGAATTA GATTGTCAAA AAGGCTATGA GCAATTAACT 
TTCTTAGAAA AGCAGAAGTT AAGAGTTCAG TATTTTCCTA TTTTAAAACT ATGTGCTGAA 
CCATCAACCG AAAGACACGT CCTGTTGGTA ACGTCTCTGA AAACCATGTT GACTTCTAAG 
GAAGAGATCA ATTTGAGTAC ACTACACACT CAAATTCGAC TTCTTCTCTC TTGTCTTTTC 
AATCAGCTAA TAGAAGTTGT AACCGAAGAT CAAGTTGATG AATCAACAAA GGGAATTTTA 
TACGAAATAA TGAGTACATT GTTTGTTTTT TCACGCGCTT TTCCCTTCCT CTTTGACTTG 
TCTTATCTAC ACCTCTTAAA ACCCTATTTA CGATCTGCTT CAACTATTGA AGAGCAACGT 
TTTCTTTACT ATGTAGTAGC TATTTTTAGA CAGGTTTTAC CATTTCAAAA AGAAATTTCT 
GAAAGCTTCC TACGGAGCCT TGAATCTGTG TTACTGCAAA GGCTTACTAA AGCCGGTACT 
GCAACGTTAA TGGAAATAGT TCCTTGTCTT TGCAGCCTAT TTACAAGACT TAATGATTAT 
GAAAGGTTAA AGAAAATTGT GGTTTCGTGT CTTAAATCTT TAGAGGAGGC ACGACACTCA 
GAAAACAATT TTCAAAAAAT GGTTCGCTTA ATAGACCTCA TTGGGCTTTT TTCTAGATAT 
GGGGATCTCA ATAGAATAAA TGATGATTGG AAACATTCAC TTGATTTTAT TAGTCCTGAG 
TGTGATGATG CTTACGTTAT ACTATTAGGT TATTTTCAGA AACTTCTAAA AGATGCGAAA 
GGTCAGCTGA GAATTCACAT AATTGATAAC ATGAGCAGAA TATGTTTAAG AGAAACATCT 
TTGTTTATAT CTCCTCTTAT GTTATCAACT TTGGATATGA TAATTGCGGA AAATAATGTT 
AACGAAGTAT CTGTTTTGTT TAAATCTTTT CTTGAGCTTT TGGCCGCGGA TGAGGATCTT 
ATCTTTGAAG CAGATCAAAA GCTAAGTTTA AAGGGTAAGC AAAACGTTCA GTCCAACAAA 
TCAGTTGATC GAGATATGCT TAAAGGAACT AAGGATAAAC AATGGATTGA AGGAGTCAGT 
GCATCTTTAA TGCAGCATTT CCTTCCCTGT ATTCTTGACT CTTGCTTTTC AAAAAATTTA 
AGGTATTCAA TGCTCGGAAT TGAAATTCTC AAATGTATTA TTCATCAAGG ACTAGTAAAC 
CCACGCATGT GTTTCTCAAC CATAATAGCA CTCGAATCCA ATGCAATTAA AGAAACCAGA 
GAAGTAGCAA TTTTGCTTCA CACTGAATTG CACCGAAGAC ATGAATCTTT GATAGATGGC 
TTATATGCTC AATCTGCAGA TTTAATATTT TCCCTTCAAA AAACGGAGGA ATATCAAACA 
TTTAAGTTAG GCGAATTTTC GCCGTTCCAA AGTGCCTATA CCATTGTCTC CGCTGATAAA 
AGTTCAAAAT CTAGGAAAAA ATTAATAATG CAAATATTAA AACCTCTTAA ACTCGACGGG 
ATTGATTTAC CCAGCTTTAC AGAAGAAAAA GTTTCATTTG TAAGCTTTTG CTGTGTATGT 
CTGGCAGGAA TTCCATACGT GTCAATAGAG GAACCGTTAA TGATCATTTC AACAGTTGAT 
TCGGTGCTCG CGACGATAGG CCCTACTATT ACTGGGTGGA TGAAAAAACT AGATCATGAA 
CGTTTTAAAA TCCTTGCAGG TATCAACTTG TGCAATTTGA TATATCTTAA GAGATATATA 
AAATACGCAT TTTCAATTTC AGACTCATCT CGACCTATAC GTGAGAAAAA GCCTCTAACT 
CTTTTGAATA GAGGTTATGT GGATTTAATT ACTTCTGATG CTAAGCCAGA TATTGTTTCT 
AAGTTGGTGA TAAAATTGTT TGAAGAAGAA AATATCCTTT CTGGCGAGGA TCAAGTTGAA 
GGGGAACAGC TTACAGTTGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
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mis4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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