ORF » Species Summary » Schizosaccharomyces pombe » dot2 cDNA ORF clone

dot2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following dot2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dot2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101232 NM_001021413.2
Latest version!		 Schizosaccharomyces pombe ESCRT II complex subunit Dot2 (dot2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    dot2 ( NM_001021413.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595503.1 Schizosaccharomyces pombe ESCRT II complex subunit Dot2 (dot2), mRNA.
    Price & Availability↑
    CloneID OSc101232
    Clone ID Related Accession (Same CDS sequence) NM_001021413.2
    Accession Version NM_001021413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ESCRT II complex subunit Dot2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021413.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCGTAAAA GAATTGGAAT TGGAGCTTTG AACGACGATG AATATCTAAA ACAGTATGAA 
    GAAGTAGGAA ATGAACTTAT TGAACAGCAG TCCGATGAAA TTGCATCTCA ACTCTCGACG 
    TTTCAGGAAG CGCTGAAGAC GTTTGCGAGA GAACATGCCA CTGAGATAAA ACAAAACTCT 
    CAATTTCGAA ATACATTTGT TAAGTTAGCT TTGAAAATTG GTTTAGATCC ATTTGTTAGT 
    GGTTCTGATG AGTCAGCTTG GGCGGCCGTG GGCATGAACG AATTCTATTA TCAGGTCGCT 
    GTACGAGTAA TCGAAGTCTG TTATGCTACT CAAATGGAGA ATGGAGGCCT CTTATCGGTC 
    AGCCAAGTTT GTCGTTTTTT AAATGAAGAA AATGAAGCCT TTGGTCATGA ATGGTTAAGA 
    GAGACTGACG TTGTTCGAGC TGTCGATAGT TTGGCCCCAC TTGGCCCTGG CTTTGTTCTT 
    GAAAAAATTG CAGGTAAACA GTATATCCGG TCCCTTCCTT TAGAGCTCAA CACCGACCAA 
    AATGTTGTTT TAGAGGCTGT AGAGATATTG GGCTATGTAA CAATATCTAT TTTACGGGAC 
    AATTACGCTT GGGAGCGTAG CAGATGCATT CAAGTATTGA ATGATTTAGT TTCAAAATCA 
    TTACTCTGGA TTGACTCCCA GGGGGTGGAG ATGGCCTATT GGGGTGCAAG CAATCTTATC 
    GACGACCAGG CGCAAAGGTT TTTATACGAA AATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595503.1
    CDS199..957
    Translation

    Target ORF information:

    RefSeq Version NM_001021413.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ESCRT II complex subunit Dot2 (dot2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001021413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCGTAAAA GAATTGGAAT TGGAGCTTTG AACGACGATG AATATCTAAA ACAGTATGAA 
    GAAGTAGGAA ATGAACTTAT TGAACAGCAG TCCGATGAAA TTGCATCTCA ACTCTCGACG 
    TTTCAGGAAG CGCTGAAGAC GTTTGCGAGA GAACATGCCA CTGAGATAAA ACAAAACTCT 
    CAATTTCGAA ATACATTTGT TAAGTTAGCT TTGAAAATTG GTTTAGATCC ATTTGTTAGT 
    GGTTCTGATG AGTCAGCTTG GGCGGCCGTG GGCATGAACG AATTCTATTA TCAGGTCGCT 
    GTACGAGTAA TCGAAGTCTG TTATGCTACT CAAATGGAGA ATGGAGGCCT CTTATCGGTC 
    AGCCAAGTTT GTCGTTTTTT AAATGAAGAA AATGAAGCCT TTGGTCATGA ATGGTTAAGA 
    GAGACTGACG TTGTTCGAGC TGTCGATAGT TTGGCCCCAC TTGGCCCTGG CTTTGTTCTT 
    GAAAAAATTG CAGGTAAACA GTATATCCGG TCCCTTCCTT TAGAGCTCAA CACCGACCAA 
    AATGTTGTTT TAGAGGCTGT AGAGATATTG GGCTATGTAA CAATATCTAT TTTACGGGAC 
    AATTACGCTT GGGAGCGTAG CAGATGCATT CAAGTATTGA ATGATTTAGT TTCAAAATCA 
    TTACTCTGGA TTGACTCCCA GGGGGTGGAG ATGGCCTATT GGGGTGCAAG CAATCTTATC 
    GACGACCAGG CGCAAAGGTT TTTATACGAA AATTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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