ppk27 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following ppk27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100854 NM_001021312.1
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Schizosaccharomyces pombe putative serine/threonine protein kinase Ppk27 (ppk27), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100854
    Clone ID Related Accession (Same CDS sequence) NM_001021312.1
    Accession Version NM_001021312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative serine/threonine protein kinase Ppk27
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCTGAAA AAAAACATTC TTGCGAAGAA AAATCGCTGC AACTATATCC TCTTTCAAAT 
    AAAATTCGTC ATGTAAATCC GAATATTTCA GCTCTTCCGT CTGAGACTGA TATATCTGAA
    TCATTGACGA ACGATTTAAC TAAACTAGAA ATTTCTAACT ATGCTCCATT TAATAGAGAT
    TTAGAGACTT TTTCCGATTT AATTTTTGGC TTAACTAATA TACAAAAAAA ATCATTGTAT
    TCTCTTCAAT TAGGCAGAAG TAGAGGATAT GCTTTATATT CAGACGAGAA TGAGAAATAT
    TTGTGGTCTA TAAATACTAA AATCACCTCT ACGGAACAAA GGGAAGTACT GCTTGTCAAA
    TCTAATAATA AGAAGTTTGA TACTTCCATC GTCCTAAAAC ATACTCACTT ATCATCCAAG
    TCTCAAAACG AAAAAAAAGC TAGAGTAAAA GTATTTCGGC AAGAAATTTG GGCCCTCAAA
    ACTTTGTCGC ATCCATGTGT TGTGCAGCTA TTAAATTACT ACGTTTCCTC CGCCGAATTA
    ATACTCGTAG AGAATTATTG TATGGGAGGA GATCTCTATC ATTACACTAA AAAACATCAT
    TCGGATTTCA GCCTTGAATT TGTTGGGCGA ATTTTTTCTG AGTTAGTACA TACAGTTGCT
    TACCTTCATT CCAAATGTTT GATTCATAGA GATTTAAAAC TTGAAAATAT ATTGCTTACC
    CAACCTTATA ATGTTATAAA GACGATTGAT AATTGGAAGA ACTACCCAAA TGCTTTAATT
    CAGATTTCTG ACTTTGAGCT TTCTATTTTT GTTGACAGTA AAAATCATTT AGTGCAATCC
    AGCTGCGGCA GCCAAGAATA TGCACCACCG GAAGTTTATA TGGGTATTGC ACATGATGGA
    TTTCGCGCGG ACGCTTGGTC TCTCGGAATA GTTTTATTTG CACTATTAGA GGGTCGTCTT
    CCCTTTGACT CCTACCCAAC TTTGGATCCT GAGAATGTAA GGATCAAACG TTATGTTCAA
    AGGCTTGTAA GATGTGATTA TACATGGCAC TTATGCAAAT CGCCATTCAA AAGATCTACT
    GGAAATACTA ATGATAACGA CGATCCTTCA TGGCGGTTCC GCTTATTTGT GAAAAAACTT
    CTCAAAAATA GAGACCAGCG CAGCACACCG ACTGAATTGT TAAAAGATTT TAATAAACAT
    GGCAACTTTA CTTTACCGTT ATTAGAGAAC GTTACGATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595405.1
    CDS1..1242
    Translation

    Target ORF information:

    RefSeq Version NM_001021312.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative serine/threonine protein kinase Ppk27 (ppk27), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCTGAAA AAAAACATTC TTGCGAAGAA AAATCGCTGC AACTATATCC TCTTTCAAAT 
    AAAATTCGTC ATGTAAATCC GAATATTTCA GCTCTTCCGT CTGAGACTGA TATATCTGAA
    TCATTGACGA ACGATTTAAC TAAACTAGAA ATTTCTAACT ATGCTCCATT TAATAGAGAT
    TTAGAGACTT TTTCCGATTT AATTTTTGGC TTAACTAATA TACAAAAAAA ATCATTGTAT
    TCTCTTCAAT TAGGCAGAAG TAGAGGATAT GCTTTATATT CAGACGAGAA TGAGAAATAT
    TTGTGGTCTA TAAATACTAA AATCACCTCT ACGGAACAAA GGGAAGTACT GCTTGTCAAA
    TCTAATAATA AGAAGTTTGA TACTTCCATC GTCCTAAAAC ATACTCACTT ATCATCCAAG
    TCTCAAAACG AAAAAAAAGC TAGAGTAAAA GTATTTCGGC AAGAAATTTG GGCCCTCAAA
    ACTTTGTCGC ATCCATGTGT TGTGCAGCTA TTAAATTACT ACGTTTCCTC CGCCGAATTA
    ATACTCGTAG AGAATTATTG TATGGGAGGA GATCTCTATC ATTACACTAA AAAACATCAT
    TCGGATTTCA GCCTTGAATT TGTTGGGCGA ATTTTTTCTG AGTTAGTACA TACAGTTGCT
    TACCTTCATT CCAAATGTTT GATTCATAGA GATTTAAAAC TTGAAAATAT ATTGCTTACC
    CAACCTTATA ATGTTATAAA GACGATTGAT AATTGGAAGA ACTACCCAAA TGCTTTAATT
    CAGATTTCTG ACTTTGAGCT TTCTATTTTT GTTGACAGTA AAAATCATTT AGTGCAATCC
    AGCTGCGGCA GCCAAGAATA TGCACCACCG GAAGTTTATA TGGGTATTGC ACATGATGGA
    TTTCGCGCGG ACGCTTGGTC TCTCGGAATA GTTTTATTTG CACTATTAGA GGGTCGTCTT
    CCCTTTGACT CCTACCCAAC TTTGGATCCT GAGAATGTAA GGATCAAACG TTATGTTCAA
    AGGCTTGTAA GATGTGATTA TACATGGCAC TTATGCAAAT CGCCATTCAA AAGATCTACT
    GGAAATACTA ATGATAACGA CGATCCTTCA TGGCGGTTCC GCTTATTTGT GAAAAAACTT
    CTCAAAAATA GAGACCAGCG CAGCACACCG ACTGAATTGT TAAAAGATTT TAATAAACAT
    GGCAACTTTA CTTTACCGTT ATTAGAGAAC GTTACGATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L