klp6 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	klp6
Entrez Gene ID	2539700
Full Name	kinesin-like protein Klp6
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713070.2	XP_001713122.1	kinesin-like protein Klp6
NM_001355876.1	NP_001342739.1	kinesin-like protein Klp6

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	klp5	NP_595703.1
Schizosaccharomyces pombe (fission yeast)	klp6	XP_001713122.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identified an alp7 mutant that had specific defects in binding to the Klp5-Klp6-PP1 complex but with normal localisation to the microtubule and kinetochore.
Title: Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement.

Fission yeast kinesin-8 contributes both to chromosome congression to the metaphase plate and to the coupling of spindle microtubules to kinetochores during anaphase A.
Title: Kinesin-8 from fission yeast: a heterodimeric, plus-end-directed motor that can couple microtubule depolymerization to cargo movement.

An MT length-dependent increase in the catastrophe frequency is mediated by kinesin-8 proteins (Klp5/6).
Title: Force- and kinesin-8-dependent effects in the spatial regulation of fission yeast microtubule dynamics.

Data suggest that Klp5 and Klp6 play a vital role in promoting microtubule dynamics, which is essential for the spatiotemporal control of microtubule morphogenesis.
Title: Fission yeast kinesin-8 Klp5 and Klp6 are interdependent for mitotic nuclear retention and required for proper microtubule dynamics.

Data show that Klp6-tail and the neck-tail domains of both motors localized at microtubule ends. Klp6-neck-tail localized to the spindle in early mitosis but to the pole-proximal ends of the spindle in anaphase B.
Title: Novel interactions of fission yeast kinesin 8 revealed through in vivo expression of truncation alleles.

The following klp6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klp6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj01023	XM_001713070.2 Latest version!	Schizosaccharomyces pombe 972h- kinesin-like protein Klp6 (klp6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSc99926	NM_001355876.1 Latest version!	Schizosaccharomyces pombe kinesin-like protein Klp6 (klp6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klp6 ( XM_001713070.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713122.1 Schizosaccharomyces pombe 972h- kinesin-like protein Klp6 (klp6), mRNA.

Price & Availability↑

CloneID	OSj01023
Clone ID Related Accession (Same CDS sequence)	XM_001713070.2
Accession Version	XM_001713070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	kinesin-like protein Klp6
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced XM_001713070.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAAAGAAG GGTCTTCAAT TTCCGTTGCA GTTCGTGTAC GACCGTTTAC TGAAAGAGAA 
AAGGGATTGC TCGCGGAAAC TCCGAAATCC AAAGAATTTC TTGGAGATGG AAGTTTGGCA 
GTGTCAAATA CATCTTCGAA TACTTTTTGT ACGAATGGAA TTCGAAAAAT TGTGCGTGTT 
TTAGACGATA ACGTGCTAAT TTTCGATCCT CCTGAAGAAA ATCCATTGGC CAAAGTCCAA 
AAATCGCTAT TACCTGCAGG GAAACGCTTT CGGGATGTTC GATATGCATT TGATCGTCTT 
TTTGGTGAAG AAGCCTCACA GGAGGATGTT TACAAGGGTA CCACAGAGCC GTTGTTGGAC 
AGTGTTTTGC AGGGATATAA TGCCACTGTG TTTGCATATG GTGCTACAGG ATGTGGTAAG 
ACACACACAA TCAGTGGTAG ACCAGACGAT CCAGGCATTA TTTTTTTAAC GATGAGGGCA 
TTGTTAGATA GAGTTGAAGG ACTCAAGCGA ACGATGAACG TCGACATTAG TGTCTCATAT 
CTTGAAATAT ATAATGAAAA GATTCGAGAT CTTTTAGTAC AGGATCCCCT TTCTATGGAA 
AAACCAAAAT CGTTAAATAT TTGTGAAGAT GCTGAGCAAA ACGTATCCGT TCCTGGTCTC 
TCATATTTTA CACCAACTAA TTTGGAAGAA GTAATGGAGA TTATTATTCG TGGAAACAGT 
AATCGAACCA TGTCACCAAC AGAAGCCAAT GCTGTTTCGT CACGCTCGCA TGCCGTTTTA 
CAGATTTATA TCACGCAAAC TCCGAAATCG GGTGAGAAGC AGGAGGAATC AGAGTCCCAA 
AATTCCCATA AAGTCCGTTC CGTATTCTCT TTTATTGATT TAGCAGGCTC AGAGAGAGCG 
AGTGCAACGA AAAATAGAGG CAAACGTTTA GTAGAAGGAG CAAACATCAA TCGGTCTTTA 
CTGGCATTGG GAAATTGCAT CAACTCGTTG TGCGAACCTC GTCGTCGACA ACATGTTCCA 
TATAGAGACT CGAAGTTGAC TCGTCTTTTA AAGTTCAGTT TAGGCGGAAA TTGCCGCACG 
TGTATGATAG TTTGTATCTC ACCGTCGTCG GAGCATTATG ATGAGACACA TAATACCTTA 
AAGTATGGCA ATCGAGCCAA AAACATCAAA ACGAAGGTTT CGAGAAACGT GGTGAGCGTA 
GATAGGCATG TAAGTGAATA TGTACGCACC ATTTACGAAT TACGACAAAA GGTTAGCATA 
CTTCAAAAGA GGATTGCTGA AGAGTCTAAG CAATTGGCTT TAAATAAGGA GGTACGAAAA 
ATTTCTTCTC GAGAAATTAA AATGTTAGAT GCTAGAAGCA TGCTCAAAAA CTCATTTGAT 
GGTTCAAGGG ATCTGCAGAA ATCACTAATT GAACATGTAC GTACATTGAG AAGGATTGAA 
GATGAAATCA CATTAACAAA GATGTGGATT TCCATTGCTA AGGAAAGTGA TGCTATGTCG 
GGGCATAATA TAAAATCTGT CGAAACAAGA CTTGCAAAGT TATACGATCA AAGAAGCTTG 
ATTACTGCCA AAGTTAATCC AGAAGAAATC TGCAAAACTT TTCAAAACAG TATTTCTCAT 
ATAGTGTCTT CGTTCAAAGG TGAGGGGGCT GATATGTATG CTGACATGTT GCAAGACGAC 
GTCGATTTGT TGAAATCTAT TATCGAAAAC CAAATTCTTG ATGCCAAGCA TGAGTCTGAA 
ACATTCTCTT CAACGTCTCG TAAGCTTATC CAGAACCTAT TCCTACTATT CCCCCTGTTA 
CCAGGAAATG CCATTGATGT TAATGAATCC CTTGCAAGGG CCTTTGACCA ATTGGTTGGA 
ATAGTACCTT CAGAGCCAAC CATTCAAGTT CCCAATTTAA TTGAAAAAGG AAAAGCACCT 
CTATTATCAA TGTTTGAAAT ACCTCGCTCT CCTAGTCGAT TCAAGGCTCG CTCACCATCC 
AAAGCTGCTC GAGTTCTAAA GAAGCCTTTG AAGAAACGAG TTCGATTTAG TGAAGTTCCC 
ACTACTAGTT CCGTACCTCC TGTTGAAATA AAGAATAAAG ATTCGAAACC GAAAGTGGAA 
AAATCTTTAG ATAAACATAA TATGAACAAC GATCGCTCTT TTCTTGTTCC TTCGAGAGAT 
GCCCGAAATA GCTTAACGTC ATTGTCATTG CATTCTAATG TCGCGAAAAA CAAAAGTTCT 
CATTCTTCCA AATGGCCAAC TCATACTCTG AGTCCTATAA TTACAACTGC ACTGAAACAA 
CCAGTACGCC GTATATCGCT TGTTTCACAA CCTTTACAAA AAACTGGCGG GACTGAGAAT 
ACTCCTAATG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713122.1
CDS	173..2527
Translation	MKEGSSISVAVRVRPFTEREKGLLAETPKSKEFLGDGSLAVSNTSSNTFCTNGIRKIVRVLDDNVLIFDPPEENPLAKVQKSLLPAGKRFRDVRYAFDRLFGEEASQEDVYKGTTEPLLDSVLQGYNATVFAYGATGCGKTHTISGRPDDPGIIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPKSLNICEDAEQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVSSRSHAVLQIYITQTPKSGEKQEESESQNSHKVRSVFSFIDLAGSERASATKNRGKRLVEGANINRSLLALGNCINSLCEPRRRQHVPYRDSKLTRLLKFSLGGNCRTCMIVCISPSSEHYDETHNTLKYGNRAKNIKTKVSRNVVSVDRHVSEYVRTIYELRQKVSILQKRIAEESKQLALNKEVRKISSREIKMLDARSMLKNSFDGSRDLQKSLIEHVRTLRRIEDEITLTKMWISIAKESDAMSGHNIKSVETRLAKLYDQRSLITAKVNPEEICKTFQNSISHIVSSFKGEGADMYADMLQDDVDLLKSIIENQILDAKHESETFSSTSRKLIQNLFLLFPLLPGNAIDVNESLARAFDQLVGIVPSEPTIQVPNLIEKGKAPLLSMFEIPRSPSRFKARSPSKAARVLKKPLKKRVRFSEVPTTSSVPPVEIKNKDSKPKVEKSLDKHNMNNDRSFLVPSRDARNSLTSLSLHSNVAKNKSSHSSKWPTHTLSPIITTALKQPVRRISLVSQPLQKTGGTENTPNA

Target ORF information:

RefSeq Version	XM_001713070.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- kinesin-like protein Klp6 (klp6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713070.2

ORF Insert Sequence:

ATGAAAGAAG GGTCTTCAAT TTCCGTTGCA GTTCGTGTAC GACCGTTTAC TGAAAGAGAA 
AAGGGATTGC TCGCGGAAAC TCCGAAATCC AAAGAATTTC TTGGAGATGG AAGTTTGGCA 
GTGTCAAATA CATCTTCGAA TACTTTTTGT ACGAATGGAA TTCGAAAAAT TGTGCGTGTT 
TTAGACGATA ACGTGCTAAT TTTCGATCCT CCTGAAGAAA ATCCATTGGC CAAAGTCCAA 
AAATCGCTAT TACCTGCAGG GAAACGCTTT CGGGATGTTC GATATGCATT TGATCGTCTT 
TTTGGTGAAG AAGCCTCACA GGAGGATGTT TACAAGGGTA CCACAGAGCC GTTGTTGGAC 
AGTGTTTTGC AGGGATATAA TGCCACTGTG TTTGCATATG GTGCTACAGG ATGTGGTAAG 
ACACACACAA TCAGTGGTAG ACCAGACGAT CCAGGCATTA TTTTTTTAAC GATGAGGGCA 
TTGTTAGATA GAGTTGAAGG ACTCAAGCGA ACGATGAACG TCGACATTAG TGTCTCATAT 
CTTGAAATAT ATAATGAAAA GATTCGAGAT CTTTTAGTAC AGGATCCCCT TTCTATGGAA 
AAACCAAAAT CGTTAAATAT TTGTGAAGAT GCTGAGCAAA ACGTATCCGT TCCTGGTCTC 
TCATATTTTA CACCAACTAA TTTGGAAGAA GTAATGGAGA TTATTATTCG TGGAAACAGT 
AATCGAACCA TGTCACCAAC AGAAGCCAAT GCTGTTTCGT CACGCTCGCA TGCCGTTTTA 
CAGATTTATA TCACGCAAAC TCCGAAATCG GGTGAGAAGC AGGAGGAATC AGAGTCCCAA 
AATTCCCATA AAGTCCGTTC CGTATTCTCT TTTATTGATT TAGCAGGCTC AGAGAGAGCG 
AGTGCAACGA AAAATAGAGG CAAACGTTTA GTAGAAGGAG CAAACATCAA TCGGTCTTTA 
CTGGCATTGG GAAATTGCAT CAACTCGTTG TGCGAACCTC GTCGTCGACA ACATGTTCCA 
TATAGAGACT CGAAGTTGAC TCGTCTTTTA AAGTTCAGTT TAGGCGGAAA TTGCCGCACG 
TGTATGATAG TTTGTATCTC ACCGTCGTCG GAGCATTATG ATGAGACACA TAATACCTTA 
AAGTATGGCA ATCGAGCCAA AAACATCAAA ACGAAGGTTT CGAGAAACGT GGTGAGCGTA 
GATAGGCATG TAAGTGAATA TGTACGCACC ATTTACGAAT TACGACAAAA GGTTAGCATA 
CTTCAAAAGA GGATTGCTGA AGAGTCTAAG CAATTGGCTT TAAATAAGGA GGTACGAAAA 
ATTTCTTCTC GAGAAATTAA AATGTTAGAT GCTAGAAGCA TGCTCAAAAA CTCATTTGAT 
GGTTCAAGGG ATCTGCAGAA ATCACTAATT GAACATGTAC GTACATTGAG AAGGATTGAA 
GATGAAATCA CATTAACAAA GATGTGGATT TCCATTGCTA AGGAAAGTGA TGCTATGTCG 
GGGCATAATA TAAAATCTGT CGAAACAAGA CTTGCAAAGT TATACGATCA AAGAAGCTTG 
ATTACTGCCA AAGTTAATCC AGAAGAAATC TGCAAAACTT TTCAAAACAG TATTTCTCAT 
ATAGTGTCTT CGTTCAAAGG TGAGGGGGCT GATATGTATG CTGACATGTT GCAAGACGAC 
GTCGATTTGT TGAAATCTAT TATCGAAAAC CAAATTCTTG ATGCCAAGCA TGAGTCTGAA 
ACATTCTCTT CAACGTCTCG TAAGCTTATC CAGAACCTAT TCCTACTATT CCCCCTGTTA 
CCAGGAAATG CCATTGATGT TAATGAATCC CTTGCAAGGG CCTTTGACCA ATTGGTTGGA 
ATAGTACCTT CAGAGCCAAC CATTCAAGTT CCCAATTTAA TTGAAAAAGG AAAAGCACCT 
CTATTATCAA TGTTTGAAAT ACCTCGCTCT CCTAGTCGAT TCAAGGCTCG CTCACCATCC 
AAAGCTGCTC GAGTTCTAAA GAAGCCTTTG AAGAAACGAG TTCGATTTAG TGAAGTTCCC 
ACTACTAGTT CCGTACCTCC TGTTGAAATA AAGAATAAAG ATTCGAAACC GAAAGTGGAA 
AAATCTTTAG ATAAACATAA TATGAACAAC GATCGCTCTT TTCTTGTTCC TTCGAGAGAT 
GCCCGAAATA GCTTAACGTC ATTGTCATTG CATTCTAATG TCGCGAAAAA CAAAAGTTCT 
CATTCTTCCA AATGGCCAAC TCATACTCTG AGTCCTATAA TTACAACTGC ACTGAAACAA 
CCAGTACGCC GTATATCGCT TGTTTCACAA CCTTTACAAA AAACTGGCGG GACTGAGAAT 
ACTCCTAATG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klp6 ( NM_001355876.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342739.1 Schizosaccharomyces pombe kinesin-like protein Klp6 (klp6), mRNA.

Price & Availability↑

CloneID	OSc99926
Clone ID Related Accession (Same CDS sequence)	NM_001355876.1
Accession Version	NM_001355876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	kinesin-like protein Klp6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGAAAGAAG GGTCTTCAAT TTCCGTTGCA GTTCGTGTAC GACCGTTTAC TGAAAGAGAA 
AAGGGATTGC TCGCGGAAAC TCCGAAATCC AAAGAATTTC TTGGAGATGG AAGTTTGGCA 
GTGTCAAATA CATCTTCGAA TACTTTTTGT ACGAATGGAA TTCGAAAAAT TGTGCGTGTT 
TTAGACGATA ACGTGCTAAT TTTCGATCCT CCTGAAGAAA ATCCATTGGC CAAAGTCCAA 
AAATCGCTAT TACCTGCAGG GAAACGCTTT CGGGATGTTC GATATGCATT TGATCGTCTT 
TTTGGTGAAG AAGCCTCACA GGAGGATGTT TACAAGGGTA CCACAGAGCC GTTGTTGGAC 
AGTGTTTTGC AGGGATATAA TGCCACTGTG TTTGCATATG GTGCTACAGG ATGTGGTAAG 
ACACACACAA TCAGTGGTAG ACCAGACGAT CCAGGCATTA TTTTTTTAAC GATGAGGGCA 
TTGTTAGATA GAGTTGAAGG ACTCAAGCGA ACGATGAACG TCGACATTAG TGTCTCATAT 
CTTGAAATAT ATAATGAAAA GATTCGAGAT CTTTTAGTAC AGGATCCCCT TTCTATGGAA 
AAACCAAAAT CGTTAAATAT TTGTGAAGAT GCTGAGCAAA ACGTATCCGT TCCTGGTCTC 
TCATATTTTA CACCAACTAA TTTGGAAGAA GTAATGGAGA TTATTATTCG TGGAAACAGT 
AATCGAACCA TGTCACCAAC AGAAGCCAAT GCTGTTTCGT CACGCTCGCA TGCCGTTTTA 
CAGATTTATA TCACGCAAAC TCCGAAATCG GGTGAGAAGC AGGAGGAATC AGAGTCCCAA 
AATTCCCATA AAGTCCGTTC CGTATTCTCT TTTATTGATT TAGCAGGCTC AGAGAGAGCG 
AGTGCAACGA AAAATAGAGG CAAACGTTTA GTAGAAGGAG CAAACATCAA TCGGTCTTTA 
CTGGCATTGG GAAATTGCAT CAACTCGTTG TGCGAACCTC GTCGTCGACA ACATGTTCCA 
TATAGAGACT CGAAGTTGAC TCGTCTTTTA AAGTTCAGTT TAGGCGGAAA TTGCCGCACG 
TGTATGATAG TTTGTATCTC ACCGTCGTCG GAGCATTATG ATGAGACACA TAATACCTTA 
AAGTATGGCA ATCGAGCCAA AAACATCAAA ACGAAGGTTT CGAGAAACGT GGTGAGCGTA 
GATAGGCATG TAAGTGAATA TGTACGCACC ATTTACGAAT TACGACAAAA GGTTAGCATA 
CTTCAAAAGA GGATTGCTGA AGAGTCTAAG CAATTGGCTT TAAATAAGGA GGTACGAAAA 
ATTTCTTCTC GAGAAATTAA AATGTTAGAT GCTAGAAGCA TGCTCAAAAA CTCATTTGAT 
GGTTCAAGGG ATCTGCAGAA ATCACTAATT GAACATGTAC GTACATTGAG AAGGATTGAA 
GATGAAATCA CATTAACAAA GATGTGGATT TCCATTGCTA AGGAAAGTGA TGCTATGTCG 
GGGCATAATA TAAAATCTGT CGAAACAAGA CTTGCAAAGT TATACGATCA AAGAAGCTTG 
ATTACTGCCA AAGTTAATCC AGAAGAAATC TGCAAAACTT TTCAAAACAG TATTTCTCAT 
ATAGTGTCTT CGTTCAAAGG TGAGGGGGCT GATATGTATG CTGACATGTT GCAAGACGAC 
GTCGATTTGT TGAAATCTAT TATCGAAAAC CAAATTCTTG ATGCCAAGCA TGAGTCTGAA 
ACATTCTCTT CAACGTCTCG TAAGCTTATC CAGAACCTAT TCCTACTATT CCCCCTGTTA 
CCAGGAAATG CCATTGATGT TAATGAATCC CTTGCAAGGG CCTTTGACCA ATTGGTTGGA 
ATAGTACCTT CAGAGCCAAC CATTCAAGTT CCCAATTTAA TTGAAAAAGG AAAAGCACCT 
CTATTATCAA TGTTTGAAAT ACCTCGCTCT CCTAGTCGAT TCAAGGCTCG CTCACCATCC 
AAAGCTGCTC GAGTTCTAAA GAAGCCTTTG AAGAAACGAG TTCGATTTAG TGAAGTTCCC 
ACTACTAGTT CCGTACCTCC TGTTGAAATA AAGAATAAAG ATTCGAAACC GAAAGTGGAA 
AAATCTTTAG ATAAACATAA TATGAACAAC GATCGCTCTT TTCTTGTTCC TTCGAGAGAT 
GCCCGAAATA GCTTAACGTC ATTGTCATTG CATTCTAATG TCGCGAAAAA CAAAAGTTCT 
CATTCTTCCA AATGGCCAAC TCATACTCTG AGTCCTATAA TTACAACTGC ACTGAAACAA 
CCAGTACGCC GTATATCGCT TGTTTCACAA CCTTTACAAA AAACTGGCGG GACTGAGAAT 
ACTCCTAATG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342739.1
CDS	173..2527
Translation	MKEGSSISVAVRVRPFTEREKGLLAETPKSKEFLGDGSLAVSNTSSNTFCTNGIRKIVRVLDDNVLIFDPPEENPLAKVQKSLLPAGKRFRDVRYAFDRLFGEEASQEDVYKGTTEPLLDSVLQGYNATVFAYGATGCGKTHTISGRPDDPGIIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPKSLNICEDAEQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVSSRSHAVLQIYITQTPKSGEKQEESESQNSHKVRSVFSFIDLAGSERASATKNRGKRLVEGANINRSLLALGNCINSLCEPRRRQHVPYRDSKLTRLLKFSLGGNCRTCMIVCISPSSEHYDETHNTLKYGNRAKNIKTKVSRNVVSVDRHVSEYVRTIYELRQKVSILQKRIAEESKQLALNKEVRKISSREIKMLDARSMLKNSFDGSRDLQKSLIEHVRTLRRIEDEITLTKMWISIAKESDAMSGHNIKSVETRLAKLYDQRSLITAKVNPEEICKTFQNSISHIVSSFKGEGADMYADMLQDDVDLLKSIIENQILDAKHESETFSSTSRKLIQNLFLLFPLLPGNAIDVNESLARAFDQLVGIVPSEPTIQVPNLIEKGKAPLLSMFEIPRSPSRFKARSPSKAARVLKKPLKKRVRFSEVPTTSSVPPVEIKNKDSKPKVEKSLDKHNMNNDRSFLVPSRDARNSLTSLSLHSNVAKNKSSHSSKWPTHTLSPIITTALKQPVRRISLVSQPLQKTGGTENTPNA

Target ORF information:

RefSeq Version	NM_001355876.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe kinesin-like protein Klp6 (klp6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355876.1

ORF Insert Sequence:

ATGAAAGAAG GGTCTTCAAT TTCCGTTGCA GTTCGTGTAC GACCGTTTAC TGAAAGAGAA 
AAGGGATTGC TCGCGGAAAC TCCGAAATCC AAAGAATTTC TTGGAGATGG AAGTTTGGCA 
GTGTCAAATA CATCTTCGAA TACTTTTTGT ACGAATGGAA TTCGAAAAAT TGTGCGTGTT 
TTAGACGATA ACGTGCTAAT TTTCGATCCT CCTGAAGAAA ATCCATTGGC CAAAGTCCAA 
AAATCGCTAT TACCTGCAGG GAAACGCTTT CGGGATGTTC GATATGCATT TGATCGTCTT 
TTTGGTGAAG AAGCCTCACA GGAGGATGTT TACAAGGGTA CCACAGAGCC GTTGTTGGAC 
AGTGTTTTGC AGGGATATAA TGCCACTGTG TTTGCATATG GTGCTACAGG ATGTGGTAAG 
ACACACACAA TCAGTGGTAG ACCAGACGAT CCAGGCATTA TTTTTTTAAC GATGAGGGCA 
TTGTTAGATA GAGTTGAAGG ACTCAAGCGA ACGATGAACG TCGACATTAG TGTCTCATAT 
CTTGAAATAT ATAATGAAAA GATTCGAGAT CTTTTAGTAC AGGATCCCCT TTCTATGGAA 
AAACCAAAAT CGTTAAATAT TTGTGAAGAT GCTGAGCAAA ACGTATCCGT TCCTGGTCTC 
TCATATTTTA CACCAACTAA TTTGGAAGAA GTAATGGAGA TTATTATTCG TGGAAACAGT 
AATCGAACCA TGTCACCAAC AGAAGCCAAT GCTGTTTCGT CACGCTCGCA TGCCGTTTTA 
CAGATTTATA TCACGCAAAC TCCGAAATCG GGTGAGAAGC AGGAGGAATC AGAGTCCCAA 
AATTCCCATA AAGTCCGTTC CGTATTCTCT TTTATTGATT TAGCAGGCTC AGAGAGAGCG 
AGTGCAACGA AAAATAGAGG CAAACGTTTA GTAGAAGGAG CAAACATCAA TCGGTCTTTA 
CTGGCATTGG GAAATTGCAT CAACTCGTTG TGCGAACCTC GTCGTCGACA ACATGTTCCA 
TATAGAGACT CGAAGTTGAC TCGTCTTTTA AAGTTCAGTT TAGGCGGAAA TTGCCGCACG 
TGTATGATAG TTTGTATCTC ACCGTCGTCG GAGCATTATG ATGAGACACA TAATACCTTA 
AAGTATGGCA ATCGAGCCAA AAACATCAAA ACGAAGGTTT CGAGAAACGT GGTGAGCGTA 
GATAGGCATG TAAGTGAATA TGTACGCACC ATTTACGAAT TACGACAAAA GGTTAGCATA 
CTTCAAAAGA GGATTGCTGA AGAGTCTAAG CAATTGGCTT TAAATAAGGA GGTACGAAAA 
ATTTCTTCTC GAGAAATTAA AATGTTAGAT GCTAGAAGCA TGCTCAAAAA CTCATTTGAT 
GGTTCAAGGG ATCTGCAGAA ATCACTAATT GAACATGTAC GTACATTGAG AAGGATTGAA 
GATGAAATCA CATTAACAAA GATGTGGATT TCCATTGCTA AGGAAAGTGA TGCTATGTCG 
GGGCATAATA TAAAATCTGT CGAAACAAGA CTTGCAAAGT TATACGATCA AAGAAGCTTG 
ATTACTGCCA AAGTTAATCC AGAAGAAATC TGCAAAACTT TTCAAAACAG TATTTCTCAT 
ATAGTGTCTT CGTTCAAAGG TGAGGGGGCT GATATGTATG CTGACATGTT GCAAGACGAC 
GTCGATTTGT TGAAATCTAT TATCGAAAAC CAAATTCTTG ATGCCAAGCA TGAGTCTGAA 
ACATTCTCTT CAACGTCTCG TAAGCTTATC CAGAACCTAT TCCTACTATT CCCCCTGTTA 
CCAGGAAATG CCATTGATGT TAATGAATCC CTTGCAAGGG CCTTTGACCA ATTGGTTGGA 
ATAGTACCTT CAGAGCCAAC CATTCAAGTT CCCAATTTAA TTGAAAAAGG AAAAGCACCT 
CTATTATCAA TGTTTGAAAT ACCTCGCTCT CCTAGTCGAT TCAAGGCTCG CTCACCATCC 
AAAGCTGCTC GAGTTCTAAA GAAGCCTTTG AAGAAACGAG TTCGATTTAG TGAAGTTCCC 
ACTACTAGTT CCGTACCTCC TGTTGAAATA AAGAATAAAG ATTCGAAACC GAAAGTGGAA 
AAATCTTTAG ATAAACATAA TATGAACAAC GATCGCTCTT TTCTTGTTCC TTCGAGAGAT 
GCCCGAAATA GCTTAACGTC ATTGTCATTG CATTCTAATG TCGCGAAAAA CAAAAGTTCT 
CATTCTTCCA AATGGCCAAC TCATACTCTG AGTCCTATAA TTACAACTGC ACTGAAACAA 
CCAGTACGCC GTATATCGCT TGTTTCACAA CCTTTACAAA AAACTGGCGG GACTGAGAAT 
ACTCCTAATG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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klp6 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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